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1 d for the detection of ERalpha in MCF-7 cell lysate.
2 protein(s) they react with in cells or cell lysate.
3 bitors of translation in rabbit reticulocyte lysate.
4 LAT1 ENE+A RNA upon addition of HEK293T cell lysate.
5 nt virus protein in the transfected BHK cell lysate.
6 ed with standard proteins and a complex cell lysate.
7 o assess hemoglobin glycation in whole blood lysate.
8 or complex mixtures of trypsin-digested cell lysate.
9 irus preferentially uses eIF4G in wheat germ lysate.
10 complex sample such as blood, serum, or cell lysate.
11 meter for three proteins and an E. coli cell lysate.
12 gh concentrations as well as of a crude cell lysate.
13 NL PBMCs in response to Mycobacterium leprae lysate.
14 e kinase activity and inhibition in the cell lysate.
15 tin aggregation in a cell model and its cell lysate.
16 plex and slow with both whole cells and cell lysate.
17 oncentrations in prostate cancer (PC-3) cell lysate.
18 s or electrophoretic mobility shift assay of lysate.
19 ical membrane antigen 1 (AMA1), and parasite lysate.
20 (IFNgamma) secretion was stimulated with CMV lysate.
21 ex biological samples such as serum and cell lysate.
22 andard deviations of up to 70% in crude cell lysate.
23 xtraction of plasmid DNA from bacterial cell lysate.
24 ulled down nucleic acids from ischemic brain lysate.
25 dvantageous to measure miRNA in a crude cell lysate.
26 de novo sequencing using whole cell E. coli lysate.
27 ough on-chip electrical sensing of bacterial lysate.
28 en when dispersed in BSA or Escherichia coli lysate.
29 mers were successfully pulled down from cell lysate.
30 ze full-length otoferlin from mammalian cell lysate.
31 by the direct detection of rRNA in bacterial lysate.
32 e the presence of growth media and Vero cell lysate.
33 cules and unpurified protein targets in cell lysates.
34 Cs as either purified enzymes or in cellular lysates.
35 ant concentrations of ERBB2/neu/Her2 in cell lysates.
36 ase activity was measured in the mouse brain lysates.
37 n endogenous protein binders in complex cell lysates.
38 and SOD activity was measured in erythrocyte lysates.
39 added in crude Escherichia coli or 293T cell lysates.
40 an 30 degrees C, when tested in reticulocyte lysates.
41 of SPSB2-iNOS interaction in macrophage cell lysates.
42 teriophage and His6-GroEL directly from cell lysates.
43 hTS with one such peptidic inhibitor in cell lysates.
44 le alpha-synuclein multimers in brain tissue lysates.
45 detection 0.3ng/mL (1.5pM) for ERBB2 in cell lysates.
46 f His6-GroEL obtained from clarified E. coli lysates.
47 he lysosomal fraction, but not in whole-cell lysates.
48 two different grades of bladder cancer cell lysates.
49 nd accumulation of processed Nrf1 in soluble lysates.
50 dividual caspases can cleave in complex cell lysates.
51 o complex aggregates, as found in total cell lysates.
52 changes in hundreds of cysteines within cell lysates.
53 ted with IGF1 receptor (IGFR1) in whole-cell lysates.
54 -purified with PfMyoA isolated from parasite lysates.
55 that are unstructured when analyzed in cell lysates.
56 AAR mutant gene libraries directly from cell lysates.
57 aration of as little as 20 mug of total cell lysates.
58 tryptase were measured in plasma and biopsy lysates.
59 ies recognized native Siglec-E within spleen lysates.
60 exposed to P. falciprum infected erythrocyte lysates.
61 to profile molecular activities within cell lysates.
62 ly active Fic(E247G) mutant in Drosophila S2 lysates.
63 y of ITPA in bacterial, yeast and human cell lysates.
64 verexpressed proteins directly in crude cell lysates.
65 avage of PrP(C) is observed in mouse retinal lysates.
66 oumarin-based fluorogenic probe in bacterial lysates.
67 ) nanotubes captured SUN1 and SUN2 from cell lysates.
68 w, as demonstrated for proteins from E. coli lysates.
69 ct cell just prior to lysis and the injected lysate 2, 5, 10, or 15 mm downstream of the injection po
70 ate that targeted analysis of unfractionated lysates (2 hr) accurately reproduces the quantification
71 n CM and coimmunoprecipitate from CM protein lysates; (2) beta1 is detected in a subset of caveolae;
72 is leads to SIRT1-mediated p53 and histone 3 lysate 56 deacetylation and results in reduced EC senesc
73 eproduces the quantification of fractionated lysates (72 hr analysis) while obviating the need for pe
74 These data demonstrate that L. rhamnosus GG lysate accelerates re-epithelialization of keratinocyte
75 apable of detecting miRNA-143 in cancer cell lysates, allowing for the discrimination between the MCF
76 ificially introduced to each analysed cell's lysate and (iii) the total variability of the expression
77 existing antibodies to asexual P. falciparum lysate and another that, based on P. falciparum serology
78 itro translation assay containing human cell lysate and purified target mRNA fused to a reporter was
79 hile the MAbs in group II bound only to VACV lysate and recombinant A27, suggesting that they recogni
81 e-molecule pull-down (SiMPull) from HEK cell lysate and subunit counting in the plasma membrane of li
83 his iBody for the isolation of FAP from cell lysates and blood serum as well as for its detection by
84 as also measured by ELISA in bronchial/nasal lysates and by immunohistochemistry in bronchial tissue
85 xpression pattern of angiogenic molecules in lysates and cell culture supernatants of prenatal skin d
88 well with activation of purified sGC in VSMC lysates and cGMP accumulation in intact porcine aortic e
89 tested varicella zoster virus-infected cell lysates and clinically isolated virus and found evidence
90 lso found that HpGroES bound to TLR4 in cell lysates and colocalized with TLR4 on the cell membrane o
91 els were also robustly increased in neuronal lysates and culture medium following lithium-VPA co-trea
92 AX-haptenated proteins were detected in cell lysates and extracellularly, either as soluble proteins
93 total protein determination in whole tissue lysates and for peptide quantification in protein digest
95 e P300/CBP-associated factor (PCAF) in liver lysates and inhibited its activity in vitro This study t
96 confirmed expression at the protein level in lysates and MSC-conditioned media by Western blot analys
98 Gag peptides were detectable in both cell lysates and supernatants in CD4+ T cells infected in vit
100 demonstrated that LH2 is present in the cell lysates and the conditioned media in a dimeric, active f
101 pliced SIV RNA and spliced SIV RNA in tissue lysates and the number of SIV RNA-positive cells in tiss
102 plex physiological environments such as cell lysates and to measure their individual activities in a
103 was verified with model samples of bacterial lysates and with four real-matrix samples of knee joint
105 at CacyBP/SIP forms a homodimer in NB2a cell lysate, and biophysical methods demonstrated that CacyBP
106 cell culture medium, rat serum, and cellular lysate, and results indicated that they are chemically a
107 geting 532 peptide precursor ions in a yeast lysate, and then 466 peptide precursors from a previousl
108 mixtures, such as tuberculin, mycobacterial lysates, and culture supernatants, all induced a similar
112 from membrane preparations when spheroplast lysates are incubated with phosphoinositide-specific pho
114 cofilin-1, and beta-actin in the whole brain lysates as well as formation of actin-cofilin rods in th
115 is broad in scope, and is applicable in cell lysates as well as to covalent inhibition/modulation of
117 ioactivity, using modified limulus amebocyte lysate assay, suggest that recombinant factor C, an LPS
121 S/MS reference mixture spiked into a complex lysate background as a function of dynamic range, includ
122 -interactive proteins from the S. coelicolor lysate based on the tandem affinity purification (TAP).
123 DH enzyme activity was detected in GV oocyte lysate, but CHDH became highly active during oocyte meio
124 on accurate miRNA quantitation in crude cell lysate by a CE-based hybridization assay termed direct q
125 The autoantigen, defined in mouse brain lysate by Western blot and mass spectrometry, was confir
126 values for unpurified proteins in crude cell lysates can be obtained without prior knowledge of the c
127 ro DISC is assembled in the presence of cell lysate, caspase-8 Y380 phosphorylation attenuates DISC a
128 dendritic cells pulsed with autologous tumor lysate combined with cyclophosphamide in patients with m
129 high quantity of mRNA from crude yeast cell lysate compared to a phenol/chloroform extraction method
131 atly increased in Nf123aIN/23aIN mouse brain lysates, confirming that exon 23a inclusion inhibits Nf1
132 as well as to complex fluids (i.e., bacteria lysates containing target proteins) results in robust an
135 Protein captured from a crude bacterial cell lysate could also be deuterated without the need for sep
136 dard data set and a public large-scale yeast lysate data set show that iTop-Q achieves highly accurat
137 mparison of adult bone marrow to fetal liver lysates demonstrated developmental silencing in IGF2BP1.
138 with recombinant METTL16, but diminished in lysate depleted of METTL16, and a supershift was detecte
139 RP gene was detected through CEST imaging of lysates derived from cells infected with G47Delta-LRP or
143 detection of multiple miRNAs in erythrocyte lysate (EL) at subpicomolar levels without need of RNA e
144 In addition, our sensor could detect SP lysate even when dispersed in BSA or Escherichia coli ly
148 Following cell lysis and sampling crude cell lysate for analysis, the substrate and the product are s
149 -scale proteomics analysis of cell or tissue lysate for enhanced proteome coverage, particularly for
150 s of natural protease substrates in cellular lysate for two understudied members: caspase-2 and caspa
152 c (Cyt c) is an important biomarker in cell lysates for the early stage of apoptosis or anticancer a
156 f both CXCR4 and CXCR7 compared with protein lysates from a nontumorigenic prostate epithelial cell l
157 aturing gels and immunoblotting, we analyzed lysates from a number of mouse tissues and cell lines to
159 y studies of BCO2 were conducted using crude lysates from bacteria or insect cells expressing recombi
160 GLD is based on GALC activity of total cell lysates from blood, which does not discriminate whether
165 duction and higher proportions of Tregs than lysates from non-ZPS-encoding relatives or a commensal s
166 Human mononuclear cells stimulated with lysates from putative ZPS-producing bacteria induce sign
168 STEP61 protein is also increased in cortical lysates from the central nervous system-specific ErbB2/4
170 We describe the generation of human platelet lysate gel (hPLG), an extracellular matrix preparation f
172 sis (HILIC-RPLC) of S. cerevisiae whole cell lysate has been used to acquire retention information fo
175 of MSP1 IgG1 (P = .022) and IgG3 (P = .023), lysate IgG1 (P = .027) and IgG3 (P = .025), AMA1 IgG1 (P
176 exhibits good sensing performance toward SP lysate in a clinically relevant linear range from 5 to 1
177 otein is 0.0078mg/ml of T24 (Grade III) cell lysate in phosphate buffered saline, artificial urine an
178 r platform by both tracking fluorescent cell lysate in sealed microwells and with a human-mouse mixed
180 t a simple platform for trapping single-cell lysates in sealed, picoliter microwells capable of print
181 eptor ligands and M. tuberculosis whole-cell lysate, increased M. tuberculosis replication, and decre
183 ate-dependent H2S production in murine liver lysate is low, consistent with a role for rhodanese in s
185 A with cyclophilin A protein in a yeast cell lysate is successfully detected and quantified using a t
186 -21 (miR21) isomiRNAs in exosomes and tissue lysates isolated from cancer-associated adipocytes (CAAs
187 irectly, we measured SHP2 activity in spleen lysates isolated from lupus-prone MRL/lpr mice and found
189 ch microwell; (iii) PAGE of each single-cell lysate; (iv) exposure of the gel to UV light to blot (im
190 ed increased activity (improved >4.5-fold in lysate; kcat increased >2.7-fold), soluble protein expre
191 stant oligomers change in size over time and lysates made from de novo induced cultures are able to c
193 cal treatments of M. tuberculosis whole-cell lysates (MtbWL) ruled out protein, nucleic acid, and non
195 a microparticulate vaccine using whole cell lysate of a murine ovarian cancer cell line, ID8 was pre
197 h anti-c-Myc antibody or C2GnT-M CT from the lysate of Panc1 cells expressing bC2GnT-M tagged with c-
199 abolome from the (12)C-/(13)C-dansyl labeled lysates of 100, 1000, and 10000 cells, respectively.
200 /(13)C-dansyl labeling of the metabolites in lysates of 100, 1000, and 10000 MCF-7 breast cancer cell
202 Similar KIR filaments were isolated from lysates of cells treated with zinc, and membrane protrus
203 on in vitro Marf accumulates in whole muscle lysates of clu-deficient flies and is destabilized upon
208 s of these molecules analyzed in whole-organ lysates of kidneys, lungs, and liver were not extensivel
210 The immunomodulatory character of pancreatic lysates of patients with cancer differs from those with
211 IL-17F protein increased in bronchial/nasal lysates of SAs and FEs and in bronchial tissue of fatal
215 iganded Pparalpha toCeacam1promoter in liver lysates ofPparalpha(+/+), but notPparalpha(-/-)mice fed
217 animals with H. pylori components (bacterial lysate or the immunomodulator VacA) and subsequently sub
219 in two distant laboratories that used boiled lysates or DNAzol-purified DNA as the template DNA.
220 d transformation results collected from cell lysates or fixed-cells conceal important dynamic informa
223 near dependence on the concentration of cell lysate present while specificity is demonstrated by comp
224 a linear range of 0.05-2.0 fmol/mug of total lysate protein and with coefficients of variation < 15%.
226 nts with GBM undergoing treatment with tumor lysate-pulsed DC vaccination and PD-1 mAb blockade were
227 emonstrated previously that autologous tumor lysate-pulsed dendritic cell-based immunotherapy in pati
228 Consolidation therapy with autologous tumor lysate-pulsed dendritic cell-based therapy and simultane
229 here that HBc expressed in a mammalian cell lysate, rabbit reticulocyte lysate (RRL), was able to as
231 s captured by directly cross-linking soluble lysates resembled those observed following multimer-PAGE
232 rotein interactions in E. coli and HeLa cell lysates, respectively, identifying 1,158 and 3,301 uniqu
233 s were captured from mouse and human cardiac lysates, respectively, including many previously unrepor
234 with DCs pulsed with Hsp70-SPIONs and tumor lysates resulted in a delayed tumor progression (as meas
236 determined for miRNA samples in a crude cell lysate, RNA extract from the lysate, and a pure buffer.
237 a mammalian cell lysate, rabbit reticulocyte lysate (RRL), was able to assemble into capsids when (lo
239 from 9 injections from a single Jurkat cell lysate sample consisting of 400 ng of total protein usin
241 d that sucrose gradient fractions from brain lysates seeded cellular tau aggregation only when large
242 Immunoprecipitation of ARC from mouse islet lysates showed ARC binds JNK, suggesting interaction bet
244 eover, the addition of purified RelE to cell lysates shows promise as a method for generating ribosom
246 earity of the calibration curves for PBS and lysate solutions at low Sur concentrations confirm the h
247 ples was assessed by testing samples of cell lysate solutions obtained from human astrocytoma (gliobl
248 ng domains (LBDs) can be recruited from cell lysates specifically onto their target phospholipids.
249 extraction of DNA from crude bacterial cell lysate spiked with 1 pg mL(-1) template DNA without requ
250 enhanced in the presence of parasitized RBC lysate, suggesting that LSK(-) cells expand and differen
251 cific activity of M. tuberculosis whole-cell lysates, suggesting that a polysaccharide was required f
252 as determined by Western blotting of vessel lysates, supporting involvement of cytoskeletal remodell
253 ing PIL-based SPME of DNA from a dilute cell lysate, the qPCR amplification efficiency was determined
254 ranslation activity of tRNA-depleted E. coli lysate to a level comparable to that of total native tRN
255 ty) to a tryptic digest of whole Jurkat cell lysate to estimate the depth of proteome coverage and to
258 IONs deliver immunogenic peptides from tumor lysates to dendritismall es, Cyrillic cells (DCs) and th
259 to purify phytase simply by heating the cell lysate, to drive aggregation of non-cyclized proteins.
260 ultistep enrichment strategy from whole cell lysate, to evaluate their abilities to enrich for differ
261 nas aeruginosa Enzymatic assays in cell-free lysate, together with crude fractionation and chemical i
264 ual SOS molecules are captured from raw cell lysate using Ras-functionalized supported membrane micro
265 be specifically pulled from K562 human cell lysates using beads decorated with phosphorylated growth
266 be assessed by fractionating tissue or cell lysates using differential ultracentrifugation through s
267 terminal unique region of dNedd4Lo in larval lysates using mass spectrometry and identified Amphiphys
268 tion by small molecules and activity in cell lysates using parallel DNA sequencing or quantitative PC
269 (1 x 10(2) CFU/ml) was detected in FCDI cell lysates using real-time PCR with greater consistency tha
270 man and mouse GBM extracellular nanovesicles lysates using the Ras-binding domain of Raf also copreci
271 Our dendritic cell-based RENCA mitochondrial lysate vaccine elicited a cytotoxic T cell response in v
272 antitation of carbonyl metabolites from cell lysate was accomplished using a carbonyl-reactive fluoro
273 lysis (SCX-RPLC) of S. cerevisiae whole cell lysate was used to generate a retention dataset of appro
274 ity of soluble PfTopoII from the translation lysates was confirmed through both a plasmid relaxation
275 of RNA from Legionella pneumophila cellular lysates was successfully performed, illustrating the use
276 nity chromatography using primary neutrophil lysate, we identify Homer3 as a novel Galphai2-binding p
277 te middle-range sized peptides from cellular lysates, we explored the use of the proteases Asp-N and
282 intracellular CD36 were found in whole brain lysates, whereas cell surface CD36 was predominantly det
283 sal TAZ S-glutathionylation in murine kidney lysates, which is elevated during ischemia/reperfusion i
284 e spectral matches enriched from mouse brain lysates, which more than triples identifications from st
285 quantitative determination of Cyt c in cell lysates, which opens a new avenue to early diagnostics a
286 a subset of HDAC8 substrates from human cell lysates, which were further validated for catalytic turn
287 urification of DNA from crude bacterial cell lysate with subsequent quantification by real-time PCR (
288 lon cancer cell lines and flash-frozen tumor lysates with a linear range of 0.05-2.0 fmol/mug of tota
289 using proteome-wide reactions of HT-29 cell lysates with a model probe of threonine beta-lactone.
290 The concentration of protein was assessed in lysates with a sensitive microfluidic antibody capture (
291 precipitation assay of mouse ameloblast cell lysates with either ameloblastin or Psma3 antibody ident
292 phosphoproteins from complex cell and tissue lysates with high specificity as confirmed by SDS-PAGE a
293 tion can be performed in blood serum or cell lysates with minimal interference from biomolecules.
294 e number of MTs (>50) from human cancer cell lysates with remarkable specificity over other classes o
295 moles of glycoproteins of interest from cell lysates, with sensitivity several orders of magnitude hi
298 European Commission) using raw mitochondrial lysates without DNA extraction or polymerase chain react
300 ies and identified 1861 proteoforms in yeast lysate, yielding an approximately 40% increase over the
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