ライフサイエンスコーパス: lysogen

1 th L5 and efficiently forms plaques on an L5 lysogen.

2 particles following induction of a phage P22 lysogen.

3 ced Nun activity and Nun antigen in an HK022 lysogen.

4 n wild-type cells with or without the SPbeta lysogen.

5 ith their host cell, forming a unit called a lysogen.

6 onstitutively transcribed phage genes in the lysogen.

7 mote positive evolutionary selection for the lysogen.

8 -gene expression system of an induced lambda lysogen.

9 a mixture of total genomic fragments from a lysogen.

10 ated when fused to lacZ and tested as single lysogens.

11 c inability of rII mutants to grow on lambda lysogens.

12 ges because it encodes a repressor and forms lysogens.

13 ne accounts for the inability of D29 to form lysogens.

14 anscript in order to allow icd expression in lysogens.

15 omains to suppress biofilm formation in DMS3 lysogens.

16 tcdA, tcdB, tcdR, tcdE, and tcdC in PhiCD119 lysogens.

17 nt switch behavior upon induction of CTX Phi lysogens.

18 e, some cells lyse whereas others survive as lysogens.

19 e can account for the lower stability of Stx lysogens.

20 phage loci in B. anthracis and/or B. cereus lysogens.

21 se variants grew lytically and formed stable lysogens.

22 eakens binding by Lac repressor also induced lysogens.

23 the Siphoviridae family and produced stable lysogens.

24 ly into its host chromosome and forms stable lysogens.

25 to the Vibrio cholerae genome to form stable lysogens.

26 e of S. enterica serovar Typhimurium Gifsy-2 lysogens.

27 ther activated eib expression in the derived lysogens.

28 Specifically, the MAV1 lysogen 158L3-1 was more virulent than the nonlysogen st

29 Upon induction of a bacteriophage lambda lysogen, a site-specific recombination reaction excises

30 Through varphi24B conversion the lysogen also gains increased antimicrobial tolerance to

31 nt expression of a small non-coding RNA in a lysogen and in late lytic growth, although it is non-ess

32 ivity is not essential for integration; both lysogens and recombination intermediates are detected wh

33 e of PhiCD119, was expressed in C. difficile lysogens and that its product, RepR, could downregulate

34 icates that Lac repressor was present in the lysogens and was necessary for stable lysogeny.

35 Nonetheless, 933W forms lysogens, and 933W prophage display a threshold response

36 This phage grows lytically, forms stable lysogens, and can switch from this regulatory state to l

37 uld grow lytically, could form highly stable lysogens, and carried out prophage induction.

38 to significantly reduce the stability of its lysogens, and may account for the hair-trigger nature of

39 s able to grow lytically, form stable single lysogens, and switch to lytic growth upon prophage induc

40 e sources in gene expression of phage lambda lysogen are quantified using models described by stochas

41 Interestingly, Lula/phi80 lysogens are recD and sbcCD phenocopies, so GamL and Agt

42 that: (i) approximately 0.005% of the H-19B lysogens are spontaneously induced per generation during

43 ase the expression of other phage genes in a lysogen because their transcripts should be terminated d

44 response to DNA damage and suggests how 933W lysogens behave as "hair triggers" with spontaneous indu

45 osmotic induction in lambdaphi(P3rpoH:lacZ ) lysogens, but had no effect on the activation of the dna

46 CI regulates its own synthesis in a lysogen by activating and repressing its promoter, P(RM)

47 ly arose via infection of an O139 CTX(ET)phi lysogen by CTX(calc)phi.

48 P(A) promoter, which is dually repressed in lysogens by the phage-encoded repressor RstR and the hos

49 ression system in which gpD deficient lambda lysogens can be co-complemented with both wild-type and

50 CTXPhi lysogens can be induced with DNA damage-inducing agents

51 Marvin is not temperate, and stable lysogens cannot be recovered from infections, although t

52 a culture spontaneously induce and when the lysogen carries two lambdoid prophages with different re

53 These lysogens carry an integrated L5 prophage inserted at a s

54 ble repressor fails to produce Stx, unlike a lysogen carrying a 933W derivative encoding a cleavable

55 A lysogen carrying a 933W derivative encoding a noncleavab

56 imit excisive site-specific recombination in lysogens carrying a single Mx8 prophage, which are less

57 nd studies utilizing Escherichia coli lambda lysogens carrying lacZ transcriptional fusions reveal th

58 which are less immune to superinfection than lysogens carrying multiple, tandem prophages.

59 nges involved in the lysis of induced lambda lysogens carrying prophages with either the lambda canon

60 e features of the new system are: (1) lambda lysogens carrying the fusion are made without regard for

61 In a lysogen, CI represses the two lytic promoters, pR and pL

62 Furthermore, E. coli lambda phage lysogens complemented with B. burgdorferi recA produced

63 of lambda and promoted curing of established lysogens, confirming that accumulation of Xis interferes

64 3 mutation confers thermoinducibility on N15 lysogens, consistent with CB being the primary repressor

65 bacteria, we used kanamycin-sensitive (KanS) lysogens containing a lambda kan- prophage.

66 Lysogens containing a single lambda kan- prophage per ba

67 ge Phi24(B) integrase expression in multiple lysogen cultures are demonstrated along with apparently

68 phi80 also did not change the phage titer in lysogen cultures, whereas the host dam mutation did incr

69 Studies with an E. coli lasB::lacZ lysogen demonstrated that RhlR multimerization was neces

70 gly, the intrinsic stability of lambdaprm240 lysogens depended markedly on the growth conditions; lys

71 g whether the HIV-1 Rev protein could direct lysogen development for bacteriophage derivatives that e

72 tion from the lambda origin was inhibited by lysogen-encoded cI repressor.

73 The derived lysogens express little or no Eib protein, in sharp cont

74 t protein nor super-repressor mutants induce lysogen formation for a P22 phage encoding an RNA hairpi

75 The wild-type coat protein directs efficient lysogen formation for P22 phages that carry several frag

76 The R17/MS2 coat protein efficiently directs lysogen formation for P22 R17 , a bacteriophage P22 deri

77 nding properties since they direct efficient lysogen formation for P22 R17 and P22 R17 [A(-10)U]; how

78 Phage lysogen formation occurs efficiently in recipient cells,

79 fe cycles after infection-host survival with lysogen formation, or host lysis and phage production.

80 HK022 prophages protect lysogens from superinfection by producing a sequence-spe

81 In CTX lysogens, gene expression originating from the rstA phag

82 depended markedly on the growth conditions; lysogens grown in minimal medium were nearly stable but

83 The SIVET lysogen has a defective H-19B prophage encoding the TnpR

84 In some cases rare lysogens have been formed in cells that belong to a muta

85 ause of a change in cI could not form stable lysogens; however, this defect could be suppressed by th

86 production of RstR(calc) renders a CTX(calc) lysogen immune to superinfection by CTX(calc)phi but sus

87 iscriminate between MC1061 and the varphi24B lysogen in standard culture, and when treated with 2 ant

88 (iv) Only a small fraction of the lysogens in a culture spontaneously induce and when the

89 Growth of H-19B lysogens in low iron concentrations or in conditions tha

90 Phage RedRock forms stable lysogens in Mycobacterium smegmatis in which the prophag

91 racterized mycobacteriophage L5 forms stable lysogens in Mycobacterium smegmatis.

92 racterized temperate phage that forms stable lysogens in Mycobacterium smegmatis.

93 eriophage L5 is a temperate phage that forms lysogens in Mycobacterium smegmatis.

94 We estimate that HK022 lysogens in stationary phase contain several hundred mol

95 Another variant could form stable lysogens in the presence of a ligand for Lac repressor b

96 yle but are apparently unable to form stable lysogens in their hosts.

97 phi in culture supernatants of El Tor CTXphi lysogens increased rapidly during exponential growth but

98 ambda lysogen with the generated lysate (the lysogen inhibits the helper phage used to package the re

99 Hence, if the repressor in a lysogen is present as a dimer, how can RecA-stimulated a

100 e high phage titer in cultures of Lula/phi80 lysogens is apparently in response to endogenous DNA dam

101 on Escherichia coli and bacteriophage lambda lysogens is reported.

102 pression-enhancing activity that the derived lysogens lack.

103 s are capable of detecting low numbers of L5 lysogens like L5 luciferase phages.

104 The lysogen-lytic viral reproduction switch is central to vi

105 disruptants grew more slowly than a control lysogen made with an att+ phage vector and gave smaller

106 Unlike wild-type lysogens, mutant lysogens were somewhat unstable under c

107 Induction of a lysogen of a lambdoid bacteriophage usually involves Rec

108 Establishment and maintenance of a lysogen of the lambdoid bacteriophage 434 require that t

109 and virulence was reexamined by creating new lysogens of 158 and of a relatively avirulent mutant, st

110 nits of a Shiga-like toxin; Escherichia coli lysogens of H-19B are converted to toxin producers.

111 Lysogens of phage HK022 are resistant to infection by ph

112 We produced isogenic Escherichia coli K-12 lysogens of seven different Shiga toxin 2 (Stx2)-encodin

113 intestinal tract, thereby demonstrating that lysogens of Shiga toxin-converting phages give rise to i

114 Lysogens of Stx phages are known to be less stable than

115 Pathogenic strains of Vibrio cholerae are lysogens of the filamentous phage CTXphi, which carries

116 xtension analysis of RNA isolated from HK022 lysogens or RNA made in vitro by transcribing a template

117 six sequences showed evidence of novel phage lysogens or sequence remnants of phage integrations, inc

118 were isolated from the environment and from lysogens, or were obtained from other laboratories.

119 expressed repressor explain why members of a lysogen population are spontaneously induced.

120 ne phosphorylation can be detected in a 933W lysogen prior to infection with HK97, while extensive St

121 pread in the laboratory environment: cryptic lysogen productivity and stealthy infectivity.

122 ically relevant context of an induced lambda lysogen, Q remains stably associated with RNAP as it tra

123 We found that dinL mutant lysogens release fewer phage in response to endogenous D

124 MAV1 DNA were cloned from three independent lysogens shown to have MAV1 DNA inserted at different si

125 As L5 lysogens spontaneously generate free phage particles, pr

126 a toxins by Stx phages is directly linked to lysogen stability because toxins are only synthesized an

127 Reduced lysogen stability can lead to increased frequency of gen

128 mbda), but this has a minimal effect on 933W lysogen stability.

129 b protein, in sharp contrast to the parental lysogen, suggesting that ECOR-9 has an expression-enhanc

130 et, restoring a functional cat gene; induced lysogens survive and are chloramphenicol resistant.

131 dolysin-dependent lysis of an induced lambda lysogen that was defective in the holin gene.

132 As a lysogen, the prophage alters the bacterial physiology by

133 In CTXphi lysogens, the activity of P(rstA), the only CTXphi promo

134 During passage of CTXphi lysogens through the infant mouse intestine, transductio

135 reporter system integrated as a single-copy lysogen to avoid titrating NtrC or polymerase.

136 uced Stx2d1 only, and supernatants from that lysogen transformed with a plasmid encoding RecA were cy

137 Lysogens underwent prophage induction upon addition of a

138 olling CI expression was weakened, rendering lysogens unstable.

139 n and viral replication are disassociated in lysogens until an induction event such as DNA damage occ

140 lasmid encoding RecA were cytotoxic when the lysogen was induced with mitomycin C.

141 Moreover, an stx(2d1)-containing lysogen was isolated from plaques on strain DH5alpha tha

142 bla mutagenesis of an Escherichia coli H-19B lysogen was undertaken.

143 Lysogens were also transformed with these vectors, by vi

144 These lysogens were capable of transducing an E. coli recipien

145 chromosomal DNA junctions from each of three lysogens were determined.

146 When lambda(imm434) lysogens were grown to mid-log or stationary phase and s

147 Unlike wild-type lysogens, mutant lysogens were somewhat unstable under certain growth con

148 to 40-fold more toxin than a pure culture of lysogens, whereas the addition of phage to phage-resista

149 lytic infection, but did produce light in L5 lysogens which are known to repress D29 promoters.

150 Infection of the 933W lysogen with a non-excluded phage fails to induce Stk-de

151 ce difference, we further compared the TIGR4 lysogen with an equally transparent variant of TIGR4 in

152 ing a recombination-deficient E. coli lambda lysogen with the generated lysate (the lysogen inhibits

153 recognize an OcRNA sequence by selecting for lysogens with a P22 R17 [Oc] phage derivative.

154 beta-galactosidase production in single-copy lysogens with appropriate genotypes.

155 The subpopulation of STEC lysogens with induced prophages has been postulated to c

156 x encoding prophages are induced compared to lysogens with non-Stx encoding prophages, suggesting inc

157 (iii) A greater fraction of lysogens with stx encoding prophages are induced compare

158 the closed-open transition probability), the lysogen would be significantly less stable.