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1 gy landscape of the L99A cavity mutant of T4 lysozyme.
2 lfide bonds of intact human insulin (HI) and lysozyme.
3 tants that are resistant to the PG hydrolase lysozyme.
4 nor changes to the native-state stability of lysozyme.
5 g an antimicrobial peptide, B. tabaci c-type lysozyme.
6 he observation of the aggregation process of lysozyme.
7 leading to increased cell susceptibility to lysozyme.
8 th lytic transglycosylases and hen egg white lysozyme.
9 istograms between spin-labels attached to T4 lysozyme.
10 hanced resistance to the antimicrobial agent lysozyme.
11 DTA, sodium ascorbate, hydrogen peroxide and lysozyme.
12 vine serum albumin, human serum albumin, and lysozyme.
13 ylbenzene, bound to an enclosed cavity in T4 lysozyme.
14 in improved resolution for the model protein lysozyme.
15 mune system, such as the cell wall hydrolase lysozyme.
16 ization of aptamers to the wine fining agent lysozyme.
17 is dependent upon sigma(V) and is induced by lysozyme.
18 ikticus is a typical enzymatic substrate for lysozyme.
19 ein model systems: barnase, spectrin, and T4 lysozyme.
20 d at a control line comprised of the protein lysozyme.
21 secreting antimicrobial proteins, including lysozyme.
22 gg phosphatidylcholine monolayer compared to lysozyme.
23 novel family of widely distributed bacterial lysozymes.
26 ereas a VLR:Tmu receptor specific to hen egg lysozyme (a self-antigen with respect to chicken B cells
28 ellular pathogen that is highly resistant to lysozyme, a ubiquitous enzyme of the innate immune syste
29 e color is formed during the fibrillation of lysozyme, a well-studied protein lacking a prosthetic gr
33 a set of 79 fibrillation curves obtained in lysozyme alone and a set of 37 obtained in the presence
34 ctivity: catalase, pyruvate kinase, albumin, lysozyme, alpha-lactalbumin, and beta-lactoglobulin.
35 first enzyme structure to be determined was lysozyme, also known as the chicken food allergen Gal d
39 exhibited strong binding (Kd </= 2 nm) with lysozyme and abrogated its lytic activity completely, th
41 plete mapping of all four disulfide bonds in lysozyme and all 17 disulfide bonds in human serum album
42 fied the ACE-binding protein in the blood as lysozyme and also a Low Molecular Weight (LMW) ACE effec
43 ilable laboratory diagnostic biomarkers (eg, lysozyme and angiotensin-converting enzyme [ACE]) are la
45 g 6 and 8 glucose moieties were observed for lysozyme and beta-casein, respectively in various heatin
47 alysis of levels of the inflammatory markers lysozyme and cathepsin S may enable detection of multipl
48 l, but these cultures were less resistant to lysozyme and cold treatments than those of the wild-type
50 y, the beta-hairpin region resembles that of lysozyme and contains an aspartate that aligns with lyso
51 d as byproducts in the industrial process of lysozyme and cystatin isolation from egg white, and (ii)
53 consisting of globular cores of myoglobin or lysozyme and demonstrate that the derived structural par
54 investigated in the L99A cavity mutant of T4 lysozyme and derivatives thereof using site-directed spi
55 sruption of decoated B. subtilis spores with lysozyme and fractionation, approximately 90% of IM fatt
56 ccurate liquid-liquid coexistence curves for lysozyme and gamma IIIa-crystallin solutions in respecti
57 enerated by tryptic and peptic digestions of lysozyme and insulin, respectively, without prior separa
59 py to study the depolarized Raman spectra of lysozyme and its complex with the inhibitor triacetylchi
63 formation of electrostatic complexes between lysozyme and low methoxyl (LM) pectin, mixtures were pre
64 We evaluated constitutive immunity testing lysozyme and natural antibody levels, and blood bacteric
66 ume changes upon unfolding of model proteins lysozyme and ribonuclease A, in solutions containing var
68 proteins, BSA, alpha-lactalbumin (alphaLA), lysozyme, and beta-lactoglobulin (betaLG), were studied
69 alt (sodium potassium tartrate), the protein lysozyme, and has been employed for routine crystallogra
70 in (IL)-6/IL-1beta, secretory IgA, and lower lysozyme, and histatins 1 and 5 were found in patients w
71 as accurately mapped to the binding cleft of lysozyme, and in a more complex example, the interaction
72 nocyte-derived macrophages, known to secrete lysozyme, and in the presence of exogenously added lysoz
73 binding of N,N',N''-triacetylchitotriose to lysozyme, and it successfully detected the binding of br
74 ein-ligand systems (maltose binding protein, lysozyme, and nitrogen regulatory protein) and obtained
75 ss lytic transglycosylases and hen egg white lysozyme, and this differentiating aspartate diminishes
76 hod gives good quantitative results; e.g., a lysozyme-antibody interaction was found to have Kd = 24
77 rphology (twisted versus flat) of fibrils of lysozyme, apo-alpha-lactalbumin, HET-s (218-289) prion,
79 levels of angiotensin converting enzyme and lysozyme are considered as inflammatory markers for diag
80 ative example shows that amyloid deposits of lysozyme are only the tip of an iceberg hiding a crowd o
81 lateral flow assays (LFAs) with biotinylated lysozyme as a model analyte in buffer and monoclonal ant
85 e and contains an aspartate that aligns with lysozyme Asp-52 (a residue critical for catalysis), a co
90 of Tregs did not occur in BAFF-Tg/Ig hen egg lysozyme BCR chimeras, demonstrating a requirement for A
91 of B cell self-reactivity, the anti-hen egg lysozyme BCR transgenic strain and the AM14 rheumatoid f
93 study is the abrogation of lytic activity of lysozyme by a novel glycosylated and surface-localized l
94 on several peptide standards and the protein lysozyme by detecting their thermal decomposition produc
96 yme, homologous to the disease-related human lysozyme, can form left-handed giant ribbons, closing in
98 M) pectin, mixtures were prepared at a fixed lysozyme concentration (0.714g.L(-1)) by progressive add
99 cific advantage against macrophage-generated lysozyme, conferred by the combination of LprI-HbN durin
100 orption spectroscopy showed modifications in lysozyme conformation during both the aggregation phase
102 n disulfide isomerase (PDI) floxed mice with lysozyme-Cre transgenic mice, we demonstrate that neutro
104 strategy developed by pathogens to resist to lysozyme degradation and thus to escape from the host in
106 eric receptors in complex with hen-egg white lysozyme demonstrate how nanomolar affinity binding of a
109 sonance (SPR) sensor for the quantitation of lysozyme dimer in monomer-dimer mixtures, reaching a det
110 other methods were used to characterize the lysozyme dimer obtained by cross-linking and confirm the
111 wlT that consists of an N-terminal bacterial lysozyme domain (N-acetylmuramidase, bLysG) and a C-term
113 ovine serum albumin, beta-lactoglobulin, and lysozyme, each of which contains multiple internal disul
114 c B cells respond to a biophysically defined lysozyme epitope displayed on both foreign and self-anti
115 ied proteolytic fragments from ovalbumin and lysozyme, exhibiting varied resistance to pepsinolysis.
117 factor RsiV is only partially degraded after lysozyme exposure, suggesting that RsiV is processed by
118 response to a high-fat diet required TLR9 on lysozyme-expressing cells, and a clinically applicable T
120 l the importance of the beta-sheet region of lysozyme for initiating self-assembly into amyloid fibri
121 to the CZE-QqQ-MS analysis, an extraction of lysozyme from cheese samples using 1 mol/L of acetic aci
126 4) report multiple HGTs of bacterial tae and lysozyme genes, respectively, to diverse eukaryotic and
128 TCR-transgenic Th cells specific to hen egg lysozyme (HEL) are adoptively transferred to recipients
129 ells, which express BCRs specific to hen egg lysozyme (HEL) display diminished responsiveness to HEL
130 e auto-Ag-specific B cells in Ig(Tg) hen egg lysozyme (HEL) transgenic mice inhabit the spleen but un
132 eic RBCs expressing the HOD antigen (hen egg lysozyme [HEL]-ovalbumin-human transmembrane Duffy(b)) w
133 antigens that are (insulin) or not (hen egg lysozyme; HEL) expressed by ss-cells have proven useful
136 hydration dynamics of crowded hen egg white lysozyme (HEWL) labeled with a metal-carbonyl vibrationa
137 an a mesophilic model protein, hen egg white lysozyme (HEWL), at all measured temperatures, opposite
140 h possesses a cytotoxic domain homologous to lysozyme, illustrating that the ferredoxin domain acts a
144 vides good fits to the cloud-point curves of lysozyme in buffer-salt mixtures as a function of the ty
145 d in secondary cell lines in the presence of lysozyme in comparison with isogenic cells expressing th
146 ng of 1,2-azaborines to model cavities in T4 lysozyme in direct comparison to their carbonaceous coun
147 model system as well as for spin-labeled T4 lysozyme in explicit water, and show how EBMetaD reprodu
148 ely with polyethylene glycol (PEG) or excess lysozyme in order to distinguish the chemically inert po
149 me, and in the presence of exogenously added lysozyme in secondary cell lines where lysozyme levels a
151 e native-state dynamical properties of human lysozyme in the context of its propensity to populate th
156 nd subsequently applied to chicken egg white lysozyme, in which one biotinylated electrochemistry (EC
157 ncreased, and that the absolute stability of lysozyme increased, whereas the stabilizing effect per m
159 gmatis (8-10-fold) protected its growth from lysozyme inhibition in vitro and enhanced its phagocytos
160 ociated with PTSD in combat veterans: PBMCs' lysozyme inhibition on the lysozyme suppression test, pl
166 clohexyl labels were attached to sites on T4 lysozyme introduced by site-directed spin labeling.
167 he leucine-99-to-alanine (L99A) mutant of T4 lysozyme is a model system that has an experimentally we
170 ere we show that during bacterial infection, lysozyme is rerouted via secretory autophagy, an autopha
171 senses the host innate immune defense enzyme lysozyme, is required for lysozyme resistance and is nec
172 ions of GIT antimicrobials, like cholate and lysozyme, leading us to hypothesize that resistance to i
173 deacetylated, we have found that exposure to lysozyme leads to additional peptidoglycan deacetylation
178 s in serum angiotensin converting enzyme and lysozyme levels of patients with ocular involvement of o
180 ils formed from pathogenic variants of human lysozyme linked with a rare but fatal hereditary systemi
181 ectrostatic interactions in the formation of lysozyme/LM pectin complexes is discussed in relation to
182 d to determine specific conditions of pH and lysozyme/LM pectin ratio for optimal complex aggregation
184 Cox-2 over-expression driven by the chicken lysozyme locus (cLys-Cox-2), which directs integration s
185 ith two homologous proteins, namely cationic lysozyme (Lys) and anionic alpha-lactalbumin (aLac), bot
187 s for the release of either Nile Red (NR) or lysozyme (LYS), loaded with gel precursors such as semi-
188 these modified electrodes, a model protein, lysozyme (Lys), was selected as the biological agent to
190 drolase family 23 (GH23), such as the g-type lysozyme (LysG) and Escherichia coli lytic transglycosyl
192 beta-lactoglobulin (betalg) and egg protein lysozyme (Lyso) were developed, using electrostatic inte
193 motrypsin (chy), bovine serum albumin (BSA), lysozyme (lyz) and cytochrome c (cyt c) in singular and
194 acid (HA), chitosan (CH)] and model protein lysozyme (LYZ) were combined in different molar and mass
195 3 gene in either myeloid cells (by using the lysozyme M [LysM] promotor) or specifically in DCs (by u
196 factor (TNF)-alpha and anti-microbial genes Lysozyme M and SLPI in the colon of Muc4(-/-) mice compa
197 ditional deletion of Stat3 in myeloid cells (lysozyme M Cre mice Stat3(fl/fl) mice), corroborating th
198 Reduced lymphangiogenesis in IL-10(-/-) and lysozyme M Cre Stat3(fl/fl) mice is associated with more
200 elete the gene in mature osteoclasts or with lysozyme M-Cre (LysM-TLN1) mice to delete TLN1 in all os
201 to delete the gene in mature osteoclasts or lysozyme M-Cre (LysM-VCL) to target all osteoclast linea
208 f cooperativity and energetics within the T4 lysozyme molecule and illuminate the fact that small cha
210 dated using calorimetry data for chicken egg lysozyme, mutated Protein A, three wild-types of haloalk
213 negative co-protein effect with addition of lysozyme, no co-protein effect with soy glycinin or egg
220 e of the bacterial inhibitor of invertebrate lysozyme, PliI-Ah, with some interesting structural vari
223 Conversely, daily oral administration of lysozyme prevented expansion of E coli during maternal s
224 romoter and several Fkh-binding sites in the lysozyme promoter, and demonstrated that Fkh-binding sit
226 common scaling of structural fluctuations of lysozyme protein embedded in different environments as t
228 fication processes in VRE that contribute to lysozyme resistance and enhanced virulence for VRE grown
229 une defense enzyme lysozyme, is required for lysozyme resistance and is necessary during C. difficile
230 se data indicate that the genes required for lysozyme resistance are highly upregulated determinants
233 Among several determinants of cholate and lysozyme resistance in E. faecalis, IreK was the only on
236 n PBP A1, we discovered that GpsB influences lysozyme resistance of L. monocytogenes as mutant strain
237 consistently demonstrated that the increased lysozyme resistance of the DeltagpsB mutant was PgdA-dep
238 can-modifying enzymes, PgdA and OatA, confer lysozyme resistance on L. monocytogenes; however, these
240 ion of novel enzymes was not responsible for lysozyme resistance, but rather, the regulation of conse
245 determination in complex with hen egg white lysozyme revealed an extended VH binding interface, with
246 peptides derived from contiguous segments of lysozyme's primary amino acid sequence revealed subdomai
247 the functional implications include loss of lysozyme secretion, reduced mucociliary clearance and ov
248 We present here the development of a novel lysozyme sensing concept based on the use of Micrococcus
250 Since pbpX and pgdA are also present in lysozyme-sensitive bacteria, this suggested that the acq
254 e course of infection revealed that the most lysozyme-sensitive strain was killed within 30 min of in
255 encoded by the lmo2769 operon that conferred lysozyme sensitivity and increased swarming but did not
257 observe the initial stages of aggregation of lysozyme solutions at 60 degrees C and pH 2, through the
262 veterans: PBMCs' lysozyme inhibition on the lysozyme suppression test, plasma cortisol decline on th
263 ein ligation technique was used to couple T4 lysozyme (T4L) and a synthetic peptide catalyst responsi
264 pared its interfacial properties to those of lysozyme that is taken as a model globular protein of si
265 e study the thermal denaturation of hydrated lysozyme that occurs when the temperature is increased.
266 t and quantify this PTM in the model protein lysozyme, the biotherapeutic filgrastim, and the Fc part
267 By using Bovine serum albumin (BSA) and lysozyme, the excellent specificity of this sensing syst
268 mutations are located in the beta-domain of lysozyme, the region that is predominantly unfolded duri
269 iate in the product release process by human lysozyme through a combination of NMR spectroscopy and m
270 sensors which are based on the detection of lysozyme through bioaffinity interactions, the bioactivi
271 ed thermal transitions, conjugation to human lysozyme through the retained end-group, and moreover sh
273 use the cavity-containing L99A mutant of T4 lysozyme to examine the pressure-induced destabilization
274 tudy, we report the reversible attachment of lysozyme to poly(ethylene glycol) (PEG) by degradable ca
277 y amyloidosis associated with the p.Trp82Arg lysozyme variant in this new family is predominantly ass
278 ient intermediate species of disease-related lysozyme variants, essentially due to the loss of global
280 cement observed upon binding of LM pectin to lysozyme was correlated with the formation of intermolec
282 lose matrix the Tm for the doubly labeled T4 lysozyme was long enough to measure an interspin distanc
283 ceptibility to pepsinolysis of ovalbumin and lysozyme was markedly enhanced by heating at 80 degrees
284 tion of apo alpha-lactalbumin (alpha-LA) and lysozyme was measured in the absence and presence of mul
285 roteins bovine insulin and chicken egg white lysozyme was observed at 4 out of 4 and 7 out of 9 of th
288 onnectivity by breaking all peptide bonds in lysozyme, we find that the hysteresis shifts towards the
290 levels of angiotensin converting enzyme and lysozyme were analyzed by enzyme-linked immunosorbent as
293 nal directionality with inhibitor binding to lysozyme, whereas the vibrational energy distribution, a
294 plasmic function sigma factor, is induced by lysozyme, which damages the peptidoglycan of bacteria.
295 complexes and refolding of betaLG, BSA, and lysozyme, while alphaLA changed to its NIS-bound state i
297 aled by our calculations comparing wild-type lysozyme with a higher catalytic rate double deletion mu
299 , we analyze the folding and unfolding of T4 lysozyme with optical tweezers under a chemo-mechanical
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