ライフサイエンスコーパス: macromolecular

1 crystallization kinetics in the presence of macromolecular additives, macromolecule incorporation, a

2 Stress granules (SGs) are large macromolecular aggregates that contain translation initi

3 This method has not yet been extended to macromolecular analytes, including proteins.

4 tion, which may be further extended to other macromolecular analytes.

5 The healthy gut restricts macromolecular and bacterial movement across tight junct

6 rate, and light adaptation, and the size and macromolecular and elemental composition of cells.

7 Additional analyses illuminate the macromolecular- and cellular-scale effects of molecular

8 Macromolecular architecture plays a pivotal role in dete

9 ficiently utilized in almost every aspect of macromolecular architecture synthesis, involving initiat

10 ne (ZnTPP), for the preparation of a complex macromolecular architecture.

11 cky-ended cohesion to create self-assembling macromolecular architectures.

12 mbled either covalently or ionically, afford macromolecular arrangements with micro- or meso-porous a

13 oscopy (AFM) to show that ordered, extensive macromolecular arrays of PSI complexes are present in th

14 data is key to understanding the function of macromolecular assemblies and complexes in their in vivo

15 his observation has wider implications where macromolecular assemblies are defined by coiled-coil pro

16 structures of many challenging yet exciting macromolecular assemblies at near-atomic resolution (3-4

17 We present an approach to study macromolecular assemblies by detecting component protein

18 a complex multistep process, which relies on macromolecular assemblies composed of 15 conserved prote

19 Our results define the macromolecular assemblies comprising the circadian feedb

20 ive mass spectrometry established that these macromolecular assemblies incorporated stoichiometric am

21 Respirasomes are macromolecular assemblies of the respiratory chain compl

22 Kinetochores are macromolecular assemblies that connect chromosomes to sp

23 ery after photobleaching (SRAP) within dense macromolecular assemblies to reveal and characterize bin

24 ndance and dynamic subcellular structures or macromolecular assemblies within such limited volumes re

25 ul structural information on many endogenous macromolecular assemblies, as we showcase on several pro

26 zymes undergo reversible polymerization into macromolecular assemblies.

27 structure determination of similarly complex macromolecular assemblies.

28 estigating the architectures and dynamics of macromolecular assemblies.

29 ning thousands of amino acids - typical in a macromolecular assembly - is tedious and time-consuming.

30 However, controlling the macromolecular assembly across length scales during solu

31 nsitive phenotypes have helped us understand macromolecular assembly and biological phenomena, yet fe

32 of the cytoplasm, which leads to widespread macromolecular assembly of proteins and triggers a trans

33 The shelterin complex is a macromolecular assembly of proteins that binds to and pr

34 acteriophage, providing unique insights into macromolecular assembly processes.

35 This syringe-shaped 3.5-MDa macromolecular assembly spans both bacterial membranes a

36 synaptonemal complex (SC) is a proteinaceous macromolecular assembly that forms during meiotic propha

37 umour microenvironments and to study dynamic macromolecular assembly, it remains challenging to image

38 22 bacteriophage capsid, a large and complex macromolecular assembly.

39 an ideal system for understanding biological macromolecular assembly.

40 utions on catalytic activity, metal binding, macromolecular binding, ligand binding, allosteric regul

41 ting step of endosomal escape in delivery of macromolecular biologic peptide, protein and siRNA thera

42 Functional macromolecular building blocks localized near the "charg

43 cles (CNP) can be achieved with counterionic macromolecular caging and decaging at the nanoscale.

44 As it progresses, the massive macromolecular catabolism dismantles the chloroplasts an

45 Desmosomes are macromolecular cell-cell junctions that provide adhesive

46 allowing for interrelation of all parametric macromolecular characteristics.

47 properties in solution is essential in basic macromolecular characterization and all research and pro

48 ion, while Protocol 3 was dedicated to their macromolecular characterization.

49 The continued expansion of the fields of macromolecular chemistry and nanoscience has motivated t

50 ules and polymers form commonplace nanoscale macromolecular compartments and bilayers, and as such ar

51 y and that the corresponding proteins form a macromolecular complex at the cytoplasmic membrane, whic

52 ochondrion, by increasing interaction with a macromolecular complex composed of the VDAC1 (voltage-de

53 gh the proposed requirement for a TRPV4-AQP4 macromolecular complex remains to be resolved.

54 RyR2 macromolecular complex remodeling, characterized by depl

55 proteins are synthesized by the ribosome, a macromolecular complex that accomplishes the life-sustai

56 The RyR1 macromolecular complex was oxidized, S-nitrosylated, Ser

57 mple of such protein assemblies, the BRCA1-A macromolecular complex, couples ubiquitin recognition an

58 bunit of ryanodine receptor subtype 2 (RyR2) macromolecular complex, which is an intracellular calciu

59 ein containing a CARD (ASC) formed cytosolic macromolecular complexes (so-called pyroptosomes) that w

60 Eukaryotic cells are densely packed with macromolecular complexes and intertwining organelles, co

61 a powerful tool for analyzing structures of macromolecular complexes and their spatial organizations

62 Spatially organized macromolecular complexes are essential for cell and tiss

63 Domains in macromolecular complexes are often considered structural

64 cognize that numerous proteins assemble into macromolecular complexes as part of normal physiology, s

65 precise structural ensembles of proteins and macromolecular complexes can be obtained with metainfere

66 lter protein-protein interactions modulating macromolecular complexes enriched in disease risk candid

67 Averaging of macromolecular complexes found within tomograms is known

68 is an important tool to study structures of macromolecular complexes in close to native states.

69 technique allows structure determination of macromolecular complexes in situ.

70 D) is the dominant method for probing intact macromolecular complexes in the gas phase by means of ma

71 bly factors facilitate the formation of many macromolecular complexes in vivo.

72 hy (ECT) provides three-dimensional views of macromolecular complexes inside cells in a native frozen

73 f-organization and that introduction of such macromolecular complexes may advance nanoengineering of

74 Macromolecular complexes may disassociate or adopt nonra

75 it is possible to obtain reconstructions of macromolecular complexes of different sizes to better th

76 -network tend to include components of large macromolecular complexes such as ribosomes and photosynt

77 i that promote the assembly of kinetochores, macromolecular complexes that bind spindle microtubules

78 ation and function, we know little about the macromolecular complexes that regulate electrical synaps

79 that p97 extracts proteins from membranes or macromolecular complexes to enable their proteasomal deg

80 as source for purifying thermostable native macromolecular complexes with an emphasis on the nuclear

81 r results suggest that endogenous NOX4 forms macromolecular complexes with calnexin, which are needed

82 ne the stoichiometry, affinity, and shape of macromolecular complexes with dissociation equilibrium c

83 o the R7 subfamily of RGS proteins that form macromolecular complexes with R7-binding protein (R7BP).

84 vious version enabling the analysis of large macromolecular complexes within a user-friendly interfac

85 of the size-distribution of macromolecules, macromolecular complexes, and nanoparticles.

86 urprising degree of functional plasticity of macromolecular complexes, and the existence of numerous

87 polyubiquitinated proteins from membranes or macromolecular complexes, but how they perform these fun

88 res organization of signaling molecules into macromolecular complexes, whose components are in intima

89 ein interactions (PPIs) regulate assembly of macromolecular complexes, yet remain challenging to stud

90 r the structural determination of biological macromolecular complexes.

91 e of local cAMP production in the context of macromolecular complexes.

92 ulation of protein functions and assembly of macromolecular complexes.

93 ined with SDS-PAGE yielded NOX4 to reside in macromolecular complexes.

94 m contains structural information of all its macromolecular complexes.

95 il aggregates to "cap" and stabilize soluble macromolecular complexes.

96 human origin that binds hyaluronan, a major macromolecular component of the eye's vitreous, with the

97 Gel-forming mucins, the primary macromolecular components of airway mucus, facilitate ai

98 arlier-evolving family members, and show how macromolecular components outside the binding motif cont

99 ises from sequence-encoded features of their macromolecular components.

100 ents micro-organisms from the level of their macromolecular components.

101 ed from this unusual environment alter their macromolecular composition, which allows the organisms t

102 stigate the three-dimensional structures and macromolecular compositions of these Golgi stacks, we ex

103 this organic material is bound in very large macromolecular compounds, analogous to the insoluble org

104 oacervates (such as spontaneous assembly and macromolecular condensation) but also assimilates the es

105 crostructural fibers without modification of macromolecular contents.

106 a powerful new click reaction for efficient macromolecular coupling.

107 Meanwhile, interactions with macromolecular crowders are favored mainly through hydro

108 solutions containing high concentrations of macromolecular crowding agents would give new insights i

109 radict predictions from accepted theories of macromolecular crowding and show that cosolutes commonly

110 can be inferred from their observations that macromolecular crowding can lead to robustness in gene e

111 ligands of different sizes (actin and ATP), macromolecular crowding can modulate the kinetics of ind

112 , ligand affinity, switching free energy and macromolecular crowding collectively control riboswitch

113 ciency, we varied the salt concentration and macromolecular crowding conditions.

114 We seek to elucidate the role of macromolecular crowding in transcription and translation

115 Our results demonstrate that macromolecular crowding in two dimensions can play a sig

116 structures because milder water deficit and macromolecular crowding induce high alpha-helix levels i

117 With increasing macromolecular crowding levels, the precision of particl

118 s generally assumed to be anomalous due high macromolecular crowding of the milieu.

119 Macromolecular crowding ought to stabilize folded forms

120 Our results show that macromolecular crowding reduces noise (as measured by th

121 robes provides more detailed readouts on the macromolecular crowding than a single sensor.

122 s on the available volume can be affected by macromolecular crowding, but the effects of crowding in

123 versatile additive in the emerging field of macromolecular cryoprotectants.

124 The majority of macromolecular crystal structures are determined using t

125 In macromolecular crystallography, the rigorous detection o

126 been supposed to be the resolution limits of macromolecular crystallography, using a method that expl

127 We identified two macromolecular, cytosolic BRAF complexes of distinct mol

128 Indeed, the macromolecular damage imposed to malignant cells by RT i

129 nse to oxidative stress that together repair macromolecular damage or direct the cell toward apoptosi

130 similarity analyses to retrieve and classify macromolecular data.

131 create hyperthermic conditions that enhance macromolecular delivery.

132 e that the existence of a maximum or optimal macromolecular density is another essential requirement.

133 The mPPCs may thus offer a general macromolecular design concept that breaks down existing

134 lectrolytes, highlighting the versatility of macromolecular design in implementing next-generation re

135 is desirable to understand how to tailor the macromolecular design of a polymer to play a passive or

136 s) reduce the effects of radiation damage on macromolecular diffraction data and thereby extend the l

137 Passive macromolecular diffusion through nuclear pore complexes

138 field of small-molecule recognition into the macromolecular domain, covering recent advances in anion

139 his study reports on polymeric CQ (pCQ) as a macromolecular drug with antimetastatic activity.

140 ike particles, dendrimers and the like, plus macromolecular drugs, antigens and/or allergens.

141 Global protein diffusion and internal macromolecular dynamics were measured using incoherent n

142 ecule methods provide direct measurements of macromolecular dynamics, but are limited by the number o

143 th the catalyzed chemistry and the protein's macromolecular electrostatics at slower time scales; tha

144 we exploit a combination of biochemistry and macromolecular EM to investigate holoenzyme assembly.

145 ehavior of individual molecules within dense macromolecular ensembles in live cells.

146 In cells, myosin and actin work in a dense macromolecular environment.

147 ermits integrated modeling of Metabolism and macromolecular Expression (ME) at genome-scale.

148 Genome-scale models of metabolism and macromolecular expression (ME) significantly expand the

149 Here we report a fully macromolecular FLP, built from linear copolymers that co

150 ial AFM enable the rapid characterization of macromolecular folding over a broader range of proteins

151 Here we show how macromolecular force sensors, acting as a genome proxy,

152 Allostery pervades macromolecular function and drives cooperative binding o

153 ietary polyallylamine (PAV) and coupled with macromolecular heparin conjugates (Corline Heparin Conju

154 d in patient therapy are characterised using macromolecular hydrodynamic techniques (dynamic light sc

155 t is one of the most important parameters of macromolecular imaging.

156 ically modified (GM) crops and are typically macromolecular in nature.

157 ter are identified to be largely composed of macromolecular inputs from degrading biomass.

158 inary structure, the transient but essential macromolecular interactions that organize the crowded ce

159 rofluidics as a powerful tool to investigate macromolecular interactions, directly related to protein

160 re cross-linked much faster than the in vivo macromolecular interactions, making them suitable for ki

161 ity, stability, subcellular localization, or macromolecular interactions.

162 ll visualization and mechanistic analysis of macromolecular interactions.

163 , D298, V300, E301 and L304), regions at the macromolecular interface between two protomers within th

164 The search for macromolecular interfaces in the Corynebacterium ammonia

165 yze the relevance of the CaFADS head-to-tail macromolecular interfaces to stabilization of assemblies

166 pling collection device that would eliminate macromolecular interference and accurately provide speci

167 The macromolecular kinetochore functions to generate interac

168 ion requires robust interactions between the macromolecular kinetochore structure and dynamic microtu

169 ver, we know little about how integrins bind macromolecular ligands in the extracellular matrix or tr

170 e bonds), links formed probabilistically and macromolecular links.

171 The ribosome is a complex macromolecular machine and serves as an ideal system for

172 The ribosome is a complex macromolecular machine composed of RNA and proteins and

173 Type VI secretion system (T6SS) is a macromolecular machine used by many Gram-negative bacter

174 ilin proteins assembled by a multi-component macromolecular machine we call the basal body.

175 ar process carried out by a highly conserved macromolecular machine, the ribosome.

176 development in higher organisms, is one such macromolecular machine.

177 e-mRNA 3' processing is carried out within a macromolecular machinery consisting of dozens of trans-a

178 The archaellum is the macromolecular machinery that Archaea use for propulsion

179 so allowed us to visualize the structures of macromolecular machines in their native context inside i

180 ssembly and function of protein-nucleic acid macromolecular machines requires multidimensional molecu

181 technique in probing the dynamics of complex macromolecular machines, we visualize the movement of in

182 systems ranging from DNA structures to large macromolecular machines.

183 anges in amide proton transfer and semisolid macromolecular magnetization transfer effects, the IDEAL

184 This enables the analysis of macromolecular mass, shape, size distribution, and inter

185 ogical transformations via a process we term macromolecular metamorphosis.

186 hnique for the real-time observation of free macromolecular migration in solution driven by centrifug

187 transition upon glucose starvation in which macromolecular mobility is dramatically restricted, redu

188 ndamental for validation and reproduction of macromolecular models and indispensable for development

189 This macromolecular modulator strategy provides an innovative

190 synthase is the most prominent bioenergetic macromolecular motor in all life forms, utilizing the pr

191 Enabling control over macromolecular ordering and the spatial distribution of

192 To investigate this massive macromolecular organelle, we generated a 'domain-level'

193 tative imaging technique with sensitivity to macromolecular organization between 20 and 200 nm, has s

194 Demonstration of similar macromolecular organization in cardiac muscle is missing

195 protein disorder in phase separation and the macromolecular organization within droplets remain elusi

196 in highly selective interactions with their macromolecular partners.

197 Bioactive macromolecular peptides and oligonucleotides have signif

198 an AmiC with increased enzymatic activity on macromolecular PG and on the synthetic PG derivative.

199 demonstrated that gonococcal AmiC can act on macromolecular PG to liberate cross-linked and non-cross

200 weight pesticides, the environmental fate of macromolecular PIPs remains less studied and is poorly u

201 duce the rapidly emerging field of synthetic macromolecular (polymer) mimics of antifreeze proteins.

202 cluding individual monomer pools, individual macromolecular pools and aggregate biomass.

203 These polysaccharides contained two major macromolecular populations; the high molecular weight po

204 The macromolecular properties of an aqueous extract of yacon

205 n yield of anionic EPS without effect on the macromolecular properties of EPS.

206 lds, residual protein concentrations and the macromolecular properties of extracted EPS were determin

207 ) but allow a better preservation of the EPS macromolecular properties.

208 l regions and lead to variants with improved macromolecular properties.

209 ature-dependent structural dynamics in large macromolecular protein assemblies.

210 ensable for maintaining the integrity of the macromolecular protein assembly, is important in enforci

211 s co-chaperones for the assembly of multiple macromolecular protein complexes.

212 tios (MTR), a marker of abnormalities in the macromolecular protein pool, in the thalami when compare

213 lts showed that the biophysical integrity of macromolecular protein pools in the posterior cingulate

214 ticularly problematic for the study of large macromolecular proteins and protein complexes.

215 itative clinically-targeted MRI method, fast macromolecular proton fraction (MPF) mapping demonstrate

216 In this work, we developed a macromolecular PS with multiple copies of mitochondria-t

217 Here, we demonstrate that macromolecular rate theory (MMRT), based on transition s

218 comparatively simpler protein scaffolds for macromolecular recognition, which can be expressed with

219 s in a near-native, frozen-hydrated state to macromolecular resolution ( approximately 4 nm).

220 kerin modules located within a non-catalytic macromolecular scaffold, whose primary role is to assemb

221 Utilizing macromolecular scaffolds as templates for the production

222 reported, coupling autocatalytic behavior to macromolecular self-assembly has been challenging.

223 amic volume, whereas IMS adds selectivity by macromolecular shape and architecture.

224 trating the assembly of postsynaptic density macromolecular signalling complex.

225 ime scales, highlighting the dynamics of the macromolecular signalling complexes in brain and periphe

226 that sources of intermediate metabolites and macromolecular sinks are tightly coupled to the cell cyc

227 distributions c(s), which further extend the macromolecular size range that can be observed in a sing

228 e peptides we selected are remarkably potent macromolecular sized pore-formers at pH 5, while having

229 Exactin shows exquisite macromolecular specificity to FX activation as compared

230 made by assessing the effects of mutation on macromolecular stability and amino acid conservation.

231 The dwell times of different macromolecular structural or functional states, correlat

232 mer sequence control achieved in nature over macromolecular structure (for example, DNA) to whole syn

233 breadth and depth of strategies for modeling macromolecular structure and dynamics for a broad audien

234 Investigation of macromolecular structure and dynamics is fundamental to

235 rly to study and simulate various aspects of macromolecular structure and dynamics.

236 been developed to provide unified access to macromolecular structure data available in the PDB and E

237 Europe accepts and annotates depositions of macromolecular structure data in the PDB and EMDB archiv

238 Serial X-ray crystallography allows macromolecular structure determination at both X-ray fre

239 phy has been the predominant method used for macromolecular structure determination.

240 Macromolecular structure files, such as PDB or PDBx/mmCI

241 olymer via an extensive rearrangement of the macromolecular structure in response to force.

242 intuitive access to improved and value-added macromolecular structure information.

243 chondrial Calcium Uniporter (MCU) complex, a macromolecular structure that guarantees Ca(2+) accumula

244 rmination of local chain dynamics within the macromolecular structure.

245 These macromolecular structures (also called egg boxes) play a

246 are caused due to formation of heterogeneous macromolecular structures and occur during condensation

247 ed directly at a surface to deliver specific macromolecular structures designed for specific function

248 ion techniques to visualize subdiffractional macromolecular structures formed by nucleic acids requir

249 ing strategy for de novo model completion of macromolecular structures from cryo-EM density maps at 3

250 comparison and clustering for large sets of macromolecular structures has become a bottleneck that n

251 Accurate atomic modeling of macromolecular structures into cryo-electron microscopy

252 Modeling of macromolecular structures involves structural sampling g

253 id growth in complexity, size, and number of macromolecular structures that are made available throug

254 l-free superresolution nanoscopic imaging of macromolecular structures with nucleotide topologies and

255 cattering, which is currently used to derive macromolecular structures, and diffuse scattering, which

256 otein domains, protein families, enzymes and macromolecular structures, as well as the life science l

257 in proteins can yield ab initio solutions of macromolecular structures, including some that elude tra

258 vidual protein complexes arranged into large macromolecular structures, termed respiratory chain supe

259 o-EM) is a key technology for elucidation of macromolecular structures.

260 imental conditions direct C-36 into distinct macromolecular structures.

261 is approach to study variation in polyhedral macromolecular structures.

262 three-dimensional (3D) lattices for solving macromolecular structures.

263 ed to determine models for three challenging macromolecular structures.

264 e is broadly applicable to studying order in macromolecular structures.

265 substrate diversity and quantity, as well as macromolecular synthesis and breakdown processes, are fu

266 nutrients to meet cellular bioenergetics and macromolecular synthesis demands of rapidly dividing cel

267 d glutamine flux to provide energy needs and macromolecular synthesis demands.

268 ngulfed contents in support of intracellular macromolecular synthesis during macropinocytosis, entosi

269 thesis using (3)H-radiolabeled precursors in macromolecular synthesis inhibition assays against MRSA.

270 cogen stores, and greatly decreases rates of macromolecular synthesis via unknown mechanisms.

271 R including inhibition of growth and related macromolecular synthesis, the extended adaptive response

272 d up new intriguing and powerful avenues for macromolecular synthesis.

273 This constitutionally dynamic macromolecular system offers the possibility of harnessi

274 des and their precursors, the development of macromolecular systems (and their applications as drug/g

275 mic helical superstructures of molecular and macromolecular systems by external stimuli is a challeng

276 Such functional nonequilibrium macromolecular systems hold great promise for on-demand

277 The study of complex macromolecular systems, however, requires an extensive c

278 mental confirmation of this hitherto unknown macromolecular target expands the bioactivity space for

279 nt L-type Ca(2+) channel was identified as a macromolecular target for (-)-englerin A.

280 Ms) that can lead to toxicity via binding to macromolecular targets (e.g., proteins or DNA).

281 Here we show that large macromolecular targets increase the rotational correlati

282 ies of binding for small-molecule ligands to macromolecular targets.

283 reveal that, in analogy to other classes of macromolecular templates such as polymer-surfactant comp

284 s were synthesized and exploited as flawless macromolecular templates that selectively rupture into a

285 Drug-free macromolecular therapeutics consisting of two self-assem

286 romolecules to facilitate the development of macromolecular therapeutics for a variety of disease ind

287 b) Drug-free macromolecular therapeutics is a new paradigm in macromo

288 a critical challenge for the development of macromolecular therapeutics or diagnostics.

289 Recently, we developed a new paradigm in macromolecular therapeutics that avoids the use of low m

290 The activity of the "drug-free macromolecular therapeutics" is based on the biorecognit

291 omolecular therapeutics is a new paradigm in macromolecular therapeutics.

292 s an effective strategy for oral delivery of macromolecular therapeutics.

293 omposed of molecular subunits with ambiguous macromolecular topology.

294 Due to its central role in macromolecular trafficking and nucleocytoplasmic informa

295 nary and compressed data representation, the MacroMolecular Transmission Format, MMTF, as well as sof

296 gh chemical permeation enhancers can improve macromolecular transport, their positive impact is often

297 ymerization or assembly of proteins or large macromolecular units by a homogeneous nucleation mechani

298 As the macromolecular version of mechanically interlocked molec

299 This creates a challenge for macromolecular visualization and analysis.

300 sitivity to crowding in vivo yields the same macromolecular volume fractions as previously obtained f