ライフサイエンスコーパス: magnesium ion

1 uce the binding of a catalytically essential magnesium ion.

2 e near a reactive tyrosine and a coordinated magnesium ion.

3 ion to 19 degrees in the presence of 3.9 mM magnesium ion.

4 ide containing two phosphates complexed with magnesium ion.

5 e that is retained in the active site by the magnesium ion.

6 as ligands in the coordination sphere of the magnesium ion.

7 This effect was not dependent upon ATP or magnesium ion.

8 so requires the presence of an HC-associated magnesium ion.

9 an inhibitory coordination of one catalytic magnesium ion.

10 regardless of the absence or the presence of magnesium ion.

11 A-1 at pH 7.4, 6.5, and 4.0 without and with magnesium ion.

12 H(3))(6)(3+) ions displace only outer sphere magnesium ions.

13 onserved Asp residues and two bound hydrated magnesium ions.

14 dressed independently by addition of zinc or magnesium ions.

15 c rate and not due to its tighter binding of magnesium ions.

16 n of junctions in the presence or absence of magnesium ions.

17 ntly depolarized to relieve channel block by magnesium ions.

18 the protein-free junction in the presence of magnesium ions.

19 l folding process, induced by the binding of magnesium ions.

20 NFalpha) mRNA was significantly inhibited by magnesium ions.

21 obe for binding sites of completely solvated magnesium ions.

22 bound zinc cation and high concentrations of magnesium ions.

23 he attacking water and may be facilitated by magnesium ions.

24 tion has long been considered to require two magnesium ions.

25 highly discriminates potassium, sodium, and magnesium ions.

26 and a DNA-bound, fully cleaved complex with magnesium ions.

27 crystal, the single catalytic site binds two magnesium ions.

28 en-stranded beta-sheet core that harbors two magnesium ions.

29 ore domain, a ssDNA GCAGT substrate, and two magnesium ions.

30 entate interactions with the two active-site magnesium ions.

31 In a first step, catalyzed primarily by magnesium ion A and its ligands, a water molecule attack

32 Although magnesium ions affect the loop conformation, they do not

33 DNA with cells and the presence of divalent magnesium ion affected transformation frequency of M. xa

34 e substitution leads to folding with reduced magnesium ion affinity in the following order: unmodifie

35 g to those that are induced by phosphate and magnesium ions alone, we also determined the structure o

36 The nonmonotonic relative motion of the magnesium ions along the reaction pathway agrees with X-

37 nique combination of an ADP molecule with no magnesium ion and a phosphate ion.

38 On the basis of our TOF-SIMS analyses of magnesium ion and Asp mapping of the mineral phase compo

39 sA is greatly facilitated by the presence of magnesium ion and ATP.

40 interactions between amino acid residues, a magnesium ion and highly ordered water molecules with th

41 he nonphosphorylated state in the absence of magnesium ion and much higher in the phospho state (near

42 found that the binding mode is modulated by magnesium ion and NaCl concentration, but unlike EcoSSB,

43 interactions involved in the binding of the magnesium ion and PAPS, thereby revealing residues criti

44 hile the other interacts with the structural magnesium ion and the ammonio groups of Lys 342 and Lys

45 d at 1.3 Angstrom resolution in complex with magnesium ion and the non-hydrolyzable substrate analog,

46 ence of SAM the coordination between a bound magnesium ion and the phosphate of A9, one of the nucleo

47 transmission is triggered by ATP capturing a magnesium ion and thereby rotating and bending a proxima

48 ng in the absence and presence of calcium or magnesium ions and a value determined by NMR spectrometr

49 r monovalent ions (sodium, potassium, Tris), magnesium ions and commonly used denaturing agents such

50 We have assigned a total of ten magnesium ions and identified a partly conserved geometr

51 aptamer relies on a combination of hydrated magnesium ions and immobilized water molecules to surrou

52 BTE folding involves cooperative uptake of magnesium ions and is driven primarily by charge neutral

53 tive site, which perturb the coordination of magnesium ions and likely affect the ability to proceed

54 catalytically significant phosphate-binding magnesium ions and provides a starting point for a detai

55 itly identify target RNA motifs sensitive to magnesium ions and SAM.

56 gesting the strong binding character of this magnesium ion, and Mg2 remains in the active site follow

57 ty depends on the concentration of ATP, free magnesium ion, and single-stranded DNA-binding (SSB) pro

58 oup II intron in the presence and absence of magnesium ions, and at a range of temperatures (298K-318

59 This water molecule is activated by two magnesium ions, and its oxygen contacts the target phosp

60 er, the roles of the catalytically essential magnesium ions, and the processes that govern the rate-l

61 ne pH conditions in the presence of zinc and magnesium ions; and (2) in vivo expression from a plasmi

62 f the F1-ATPase from C. thermarum, ATP and a magnesium ion are bound to the alpha-helices in the down

63 nt with the previous studies, two well-fixed magnesium ions are coordinated by five active site resid

64 Based on these studies, it is clear that magnesium ions are crucial for stabilizing the folded st

65 anning counter-ion condensation and explicit magnesium ions are employed to calculate the folding fre

66 Magnesium ions are essential to the basic metabolic proc

67 cific DNA complexes formed in the absence of magnesium ions are highly stable.

68 nating to bases via its hydration shell, two magnesium ions are located at the periphery of the minor

69 around the catalytic and nucleotide binding magnesium ions are mismatch specific.

70 In both structures, two magnesium ions are observed.

71 Co(NH3)63+ concentrations, a fraction of the magnesium ions are trapped as actin filaments aggregate.

72 the cooperativity and apparent affinity for magnesium ions as a function of changes in base sequence

73 a saturating total concentration (20 mM) of magnesium ion at 27 degrees C and pH 7.5.

74 one of GDP, and one of taxol, as well as one magnesium ion at the non-exchangeable nucleotide site, a

75 tructure of RnPIP complexed with AMP, Pi and magnesium ions at 1.69 A resolution provides insight int

76 nd r(G)d(GCGTATACGC) with bound hexahydrated magnesium ions at high resolution.

77 MP and PP(i), suggesting the presence of two magnesium ions at the catalytic site of EF.

78 like human PBGS, the TgPBGS octamer contains magnesium ions at the intersections between pro-octamer

79 alyzed in terms of the sequential binding of magnesium ions at two sites.

80 aving group forms an alkoxide coordinated to magnesium ion B.

81 These results support the hypothesis that magnesium ions bind loosely and nonspecifically to actin

82 In all cases, specific and non-specific magnesium ion binding accompanies folding into the activ

83 at the functional groups in D5 implicated in magnesium ion binding and catalysis (catalytic triad, in

84 Instead the manganese and magnesium ion binding appears to be associated with the

85 nd we propose two possible locations for the magnesium ion binding site(s).

86 d, suggesting that stem-loop I is a possible magnesium ion binding site.

87 ally important phosphates, many of which are magnesium ion binding sites, in diverse group I introns,

88 he sole exception was traced to differential magnesium ion binding.

89 scribing the linkage between RNA folding and magnesium ion binding.

90 operativity and reduced affinity of apparent magnesium ion binding.

91 nges resulting from fluoride, phosphate, and magnesium ions binding to those that are induced by phos

92 s the tertiary folding of RNA often requires magnesium ions binding to tight places where phosphates

93 A magnesium ion bound by these aspartate residues could th

94 rovides a high-resolution view of a hydrated magnesium ion bound in a similar manner to a divalent ca

95 ration experiments showing approximately 1.5 magnesium ions bound per complex.

96 by specifically increasing the affinities of magnesium ions bound to the RNase P x pre-tRNA(Asp) comp

97 mission rate was enhanced by the presence of magnesium ions but was inhibited by calcium ions.

98 um or hexammine cobalt (III) ions as well as magnesium ions, but the transition rates are higher for

99 genase or lactic dehydrogenase) or even 3 mM magnesium ions can also cause lipid mixing.

100 sine 5'-triphosphate in solutions containing magnesium ions can be treated by considering 17 species.

101 ains two metal ions, consistent with the two-magnesium ion catalytic mechanism.

102 chanism for bond cleavage in which C75 and a magnesium ion catalyze the reaction by general acid-base

103 Magnesium ion channels and transporters regulate the cel

104 abilizes P22 virions against disruption by a magnesium ion chelating agent.

105 lished strategy for fluorescence emission on magnesium ion chelation by phosphorylated peptides carry

106 The structure also reveals a four-magnesium-ion cluster involved in both catalysis and pos

107 on reaction and are likely to be involved in magnesium ion co-ordination.

108 nteresting interactions with bases, hydrated magnesium ions, cobalt(III)hexaammine, spermine, and wat

109 oduct formation, apparently by ligation of a magnesium ion cofactor.

110 t not growth, is modulated by nucleotide and magnesium ion cofactors.

111 and K505E (PLRGKILNVR) increase the optimal magnesium ion concentration for strand passage, without

112 edly, helicase activity is optimal at a free magnesium ion concentration of 0.05 mm.

113 for the production of isochorismate, a high magnesium ion concentration suppresses the rate of relea

114 Here, we use an experimental perturbation of magnesium ion concentration that disrupts the folding of

115 r a stressful environmental condition of low magnesium ion concentration, but preserve the phenotype

116 ers was measured under conditions of optimal magnesium ion concentration.

117 pairs was also investigated as a function of magnesium ion concentration.

118 For magnesium ion concentrations above 1 mM, the interstem a

119 (2) At magnesium ion concentrations optimal for splicing (20 mM

120 ecA C terminus imposes the need for the high magnesium ion concentrations requisite in RecA reactions

121 elf-splicing in vitro (42 degrees C and high magnesium ion concentrations).

122 Our CEST and SAXS experiments, at different magnesium ion concentrations, quantitatively confirm our

123 ogous DNA pairing and strand exchange at low magnesium ion concentrations.

124 omal 70S complex against dissociation at low magnesium ion concentrations.

125 Whether similar RNA-RNA and RNA-magnesium ion contacts play related functional roles in

126 We elucidated thereby how the magnesium ion contributes to catalysis.

127 For example, in Ras the magnesium ion contributes to the catalysis of GTP hydrol

128 of the theoretical IR-difference spectra for magnesium ion coordinated triphosphate to experimental o

129 s), a hydrophobic C-terminal region, and two magnesium ions coordinated in the palm domain.

130 us crystallographic studies showed that both magnesium ions coordinated to the carboxylate group of t

131 yme at the ligation site, where an essential magnesium ion coordinates three phosphates.

132 ransferases contains a DxD motif involved in magnesium ion coordination for catalysis.

133 lic character at C1 of the farnesyl chain by magnesium ion coordination of the pyrophosphate leaving

134 rtate residues of the IMTD(Q/A)DXD motif for magnesium ion coordination, and we propose two possible

135 rotein residues in the vicinity of dNTP, and magnesium ions coordination) during nucleotide discrimin

136 Although these data suggest that magnesium ions decrease the binding of E2 to non-specifi

137 e a bend angle in the presence or absence of magnesium ion, demonstrates that magnesium ion markedly

138 is quantitative demonstration of substantial magnesium ion dependence has several important implicati

139 Further dissection of the magnesium ion dependence of individual reaction steps re

140 The magnesium ion dependence of the catalytic parameters of

141 ATPase activity was characterized as magnesium ion dependent, vanadate sensitive, and slightl

142 In the second, magnesium ion-dependent reaction, diphosphate ester ioni

143 uch as zinc, sodium, potassium, calcium, and magnesium ions did not affect the luminescence of thiogl

144 least three magnesium ions, with an apparent magnesium ion dissociation constant of 16mM, but K(M) wa

145 l estrogen hydroxyl groups and the catalytic magnesium ion, distorting the active site and trapping t

146 Magnesium ions do not affect the affinity of either the

147 Effects of magnesium ions dominate and determine duplex stability a

148 lly, the product metal site is occupied by a magnesium ion early in the pyrophosphorolysis reaction p

149 Magnesium ions enhance farnesylation catalyzed by FTase

150 which contain an excess of non-specific DNA, magnesium ions enhance the binding of E2 to its specific

151 step distance, while the G1-N7 coordinates a magnesium ion essential for the activation of the nucleo

152 It has become increasingly clear that magnesium ions exhibit an important function through kin

153 In the Neurospora VS ribozyme, magnesium ions facilitate formation of a loop-loop inter

154 study established that Btk requires a second magnesium ion for activity.

155 e catalytic metal site must be occupied by a magnesium ion for pyrophosphorolysis to occur.

156 deletion, this decreased ability to utilize magnesium ions for catalysis appears to be due to locali

157 P (RNase P) requires high concentrations of magnesium ions for efficient catalysis of tRNA 5'-matura

158 ved tyrosine as its active site and required magnesium ions for its relaxation activity.

159 s also found that the presence or absence of magnesium ion had little effect on the value of the link

160 odest increase in affinity in the absence of magnesium ions (half-maximal saturation 6.1 +/- 1.1 nm).

161 The structure reveals that potassium and magnesium ions have an unexpected yet significant struct

162 ontrast, in the absence of non-specific DNA, magnesium ions have no effect on the binding of E2 to sp

163 a7 loop is critical for the binding, and the magnesium ion held within the porphyrin is coordinated b

164 ents of the nucleotide-binding and catalytic magnesium ions help guide polymerase selection for the c

165 .4-fold upon cooperative binding of a second magnesium ion (Hill coefficient 2.5 +/- 0.5), suggesting

166 ine the location of the putative active-site magnesium ion, however, no evidence for the metal ion wa

167 magnesium levels, and a reduced affinity for magnesium ions important for catalysis.

168 the role of invariant Lys557 and a divalent magnesium ion in coordinating the ATP pyrophosphates, as

169 The coordination of the magnesium ion in proteins by triphosphates plays an impo

170 phosphate groups and coordinating the second magnesium ion in the active site, respectively.

171 the N-7 position assists in coordinating the magnesium ion in the active site.

172 reorganization is mediated by the catalytic magnesium ion in the active site.

173 one with no metal ions and another with one magnesium ion in the catalytic site.

174 characteristics suggest that Gfh1 chelates a magnesium ion in the RNA polymerase active site (like Gr

175 activated for nucleophilic attack and why a magnesium ion in the third metal site is required for ca

176 uplex and the first observation of localized magnesium ions in a quadruplex structure.

177 be related to the strong solvation shells of magnesium ions in aqueous media.

178 This study investigates the role of magnesium ions in coupling ATP hydrolysis to the nucleic

179 mics (MD) simulations addressing the role of magnesium ions in FTase are presented, and relevant octa

180 +) and Li(+) selectivity against calcium and magnesium ions in mixed solutions is improved by 4 and >

181 The ubiquitous presence of magnesium ions in RNA has long been recognized as a key

182 nding pockets, and the distribution of bound magnesium ions in RNA structures.

183 crystallography, we show that LGK binds two magnesium ions in the active site that are additionally

184 gave dissociation binding constants for the magnesium ions in the millimolar range, similar to the b

185 m our simulation results, demonstrating that magnesium ions induce collapse and pre-organization.

186 Magnesium ions induce folding into the active conformati

187 the thermodynamics of magnesium binding and magnesium ion-induced folding of the ribozyme.

188 We found that excess zinc or magnesium ion inhibited the formation of virus-like part

189 Using chemical-shift mapping, we show that magnesium ions interact with the loop of the isolated st

190 uggest that a previously unobserved hydrated magnesium ion interacts with N7 of the cleavage site G.U

191 The magnesium ion interacts with the beta and gamma-phosphat

192 the energetically unfavorable insertion of a magnesium ion into a porphyrin ring.

193 to the active site flap, in which a putative magnesium ion is coordinated by a glutamate carboxyl and

194 In FTase, the magnesium ion is coordinated by aspartate beta352 and th

195 The magnesium ion is coordinated by the carboxylates of Asp

196 The presence of magnesium ion is essential for this ribozyme to exhibit

197 A magnesium ion is found to be coordinated to the phosphat

198 The structure suggests that inclusion of a magnesium ion is important for stabilizing the position

199 e that, unlike the HNF-3gamma/DNA complex, a magnesium ion is not required in forming the stable Gene

200 The essential magnesium ion is observed bridging the phosphate groups

201 The magnesium ion is proposed to coordinate the diphosphate

202 We provide evidence that the free magnesium ion is required to mediate a conformational ch

203 tains the sulfate (or phosphate) ion and two magnesium ions is in the closed conformation observed in

204 A magnesium ion located at the junction of the two pseudok

205 absence of magnesium ion, demonstrates that magnesium ion markedly increases the bend angle.

206 suggest that specialized macromolecules and magnesium ions may cooperate in the stabilization of int

207 It is proposed that magnesium ions may promote conformational change that fa

208 Magnesium ions may serve as a physiological cofactor wit

209 lzheimer's disease (AD) is associated with a magnesium ion (Mg(2+)) deficit in the serum or brain.

210 han the natural divalent cation of the cell, magnesium ion (Mg(2+)).

211 tein MAGT1 participates in the intracellular magnesium ion (Mg) homeostasis and facilitates a transie

212 Magnesium ions (Mg(2+)) are essential for life, but the

213 occurring polyphosphates (PIP2 and ATP) and magnesium ions (Mg(2+)).

214 The magnesium ion, Mg(2+), is essential for all life as a co

215 hythms in the intracellular concentration of magnesium ions, [Mg(2+)]i, which act as a cell-autonomou

216 Renal magnesium ion (Mg2+) resorption occurs predominantly thr

217 Magnesium ions (Mg2+) play an important role in biochemi

218 ganese ions (Mn2+) are preferred rather than magnesium ions (Mg2+), whereas zinc ions (Zn2+) inhibit

219 e spectroscopy measurements that substantial magnesium ion mobility can indeed be achieved in close-p

220 oretical predictions also indicate that high magnesium ion mobility is possible in other chalcogenide

221 a key residue for catalysis coordinating the magnesium ion, moves closer, presumably switching nucleo

222 In addition to a magnesium ion needed to form the ATP-Mg complex, we have

223 These data revealed that all of the magnesium ions observed in the structural model are impo

224 the first time the effect of the presence of magnesium ion on the bend angle conferred by an A-tract.

225 ge degree of buffering, while competition of magnesium ions on Ni uptake was observed even in unbuffe

226 ay account for the stimulatory properties of magnesium ions on the enzyme.

227 ulations, we calculate the effect of SAM and magnesium ions on the folding free energy landscape of t

228 and the thermodynamic stabilizing effect of magnesium ions on the RNA structure.

229 s occupied by the product ADP, but without a magnesium ion or phosphate, providing evidence that the

230 del for the RecA-ADP complex did not include magnesium ion or side chains.

231 The number of released magnesium ions per phosphate charge is sequence dependen

232 metal-activated metalloenzyme which uses two magnesium ions per subunit: the strongly bound conformat

233 Magnesium ions permeate the channel and transjunctional

234 Magnesium ions play a crucial role in the structural int

235 Magnesium ions play important roles in the structure and

236 ons of polymerase beta, we find that a third magnesium ion positioned near the newly identified produ

237 oducts in the crystal lattice, implicate one magnesium ion, previously termed Mg2, as the more stably

238 siologically relevant rate, because a second magnesium ion promotes both MgATP2- binding and phosphor

239 Biochemical data suggest that the magnesium ions provide structural stability and are dire

240 ific DNA complexes formed in the presence of magnesium ions rapidly results in the formation of E2-sp

241 y packing interactions and further uptake of magnesium ions relative to the state in high Mg(2+) but

242 We use this analysis to predict that magnesium ions remodel the landscape, shifting the equil

243 ter total time, and the presence of divalent magnesium ions (replacing sodium) reduces the pressure,

244 dramatically decreases the concentration of magnesium ions required for the formation of an active s

245 A single magnesium ion resides in the putative active site cavity

246 .3, -4.4, and -7.1 for lithium, calcium, and magnesium ions, respectively).

247 forms 1:3 and 1:2 complexes with calcium and magnesium ions, respectively.

248 t is likely that the binding affinity of the magnesium ion(s) specifically required for DNA cleavage

249 Additionally one of the magnesium ions serves to activate the leaving group (the

250 GH3-1 is a monomer in solution and requires magnesium ions solely for the adenlyation reaction.

251 The results show that magnesium ions stabilize the stacked-X form and destabil

252 Magnesium ions strongly influence the structure and bioc

253 m experiments at different concentrations of magnesium ions suggest that Mss 116 stimulates folding o

254 A is more efficient at low concentrations of magnesium ions, suggesting that partially unfolded RNA i

255 ilic and widely open to the cytoplasm with a magnesium ion surrounded by four highly conserved aspart

256 An adjacent residue, Asp290, binds a magnesium ion that forms a bridge to ATP, reorienting th

257 not alter the number or apparent affinity of magnesium ions that are either diffusely associated with

258 on binding is inhibited by concentrations of magnesium ions that are sufficient to "stack" the X-junc

259 as well as the observations of potassium and magnesium ions, the crystal structure has revealed a hig

260 In the absence of cofactor magnesium ions, the EcoRI conjugates bind to specific se

261 al and transformed cells, in the presence of magnesium ions, the majority of the 34-kd protein is ass

262 ingly different responses to the addition of magnesium ions: the interstem angle for yeast tRNAPhe is

263 at protein-based pores can allow exchange of magnesium ions through the vesicle wall while keeping th

264 eases from about 7 degrees in the absence of magnesium ion to 19 degrees in the presence of 3.9 mM ma

265 can be activated by the direct binding of a magnesium ion to the enzyme, in addition to ATP-complexe

266 13M, K367M, and E429A/E429Q) or coordinating magnesium ions to ATP (E148A/E148Q, N150A/N150D, and E37

267 scaffolds for the binding and positioning of magnesium ions to catalyze phosphodiester bond hydrolysi

268 rminal lengths in the presence or absence of magnesium ions to determine the impact on enzyme propert

269 ic evidence that ERK2 must bind two divalent magnesium ions to facilitate catalysis at a physiologica

270 coordinate the viral RNA template, NTPs and magnesium ions to facilitate nucleotide condensation(1).

271 coordinate the viral RNA template, NTPs, and magnesium ions to facilitate nucleotide condensation1.

272 st be interpreted as non-specific binding of magnesium ions to the inosine-containing RNA oligonucleo

273 ty was related to the specific adsorption of magnesium ions to the negatively charged SiO2 surface.

274 actococcus lactis JBB 500 were enriched with magnesium ions using Pulsed Electric Fields.

275 ntaining a restriction enzyme (Sma 1), caged magnesium ions (using a DM-Nitrophen complex), and a che

276 Recently, a third active site magnesium ion was identified in some DNA polymerase prod

277 Efficient transfer of sodium and magnesium ions was readily observed with significant red

278 f calcium ions was globular, while that with magnesium ions was rod-shaped.

279 ration of the chelating agent to that of the magnesium ions was used to control the radius of this re

280 The competitive effects of potassium and magnesium ions were characterized.

281 ular location on the stretched DNA molecule, magnesium ions were released into solution.

282 whether all or a subset of the RNA-chelated magnesium ions were required for riboswitch function.

283 498, D500 and D502, thought to co-ordinate a magnesium ion, were mutated to alanine; in addition, the

284 o dimerize with or without either calcium or magnesium ions when analyzed by light scattering or anal

285 bosomes is sensitive to the concentration of magnesium ions when Rei1 is absent.

286 reduced by nearly 50 % upon addition of 2 mM magnesium ions, whereas the angle for mtRNASer(AGY) incr

287 me does not have an absolute requirement for magnesium ions, whereas TrmD requires magnesium to expre

288 contacts to the water shell of an associated magnesium ion, which bridges fluoroquinolone-gyrase inte

289 ain of Ras binds GTP or GDP accompanied by a magnesium ion, which is strictly required for GTPase act

290 ates/phosphorothiolate indicate that the two magnesium ions, which constitute essential co-factors, a

291 Our data are centered on the role of magnesium ions, which inhibit the isochorismate synthase

292 ide ion bridges the structural and catalytic magnesium ions while the other interacts with the struct

293 , the molecular basis of the interactions of magnesium ions with RNA helical regions is less well und

294 ft perturbation mapping to interact with the magnesium ion, with apparent K(d) values in the micromol

295 nding is also facilitated by the presence of magnesium ions, with a nanomolar apparent dissociation c

296 d to depend on the binding of at least three magnesium ions, with an apparent magnesium ion dissociat

297 ing to proceed at a reduced concentration of magnesium ions, with double substitutions more effective

298 The structure revealed the coordination of a magnesium ion within the catalytic core comprised of the

299 p190, Asp192, Asp256) and the two functional magnesium ions; without the catalytic ion, other protein

300 he isocyclic E ring, omission of the central magnesium ion, zinc as an alternative metal ion, and a n