ライフサイエンスコーパス: mammalian cell

1 . paralogs) are critical for efficient HR in mammalian cells.

2 eir lethality and can damage mitochondria in mammalian cells.

3 cytes, as well as their cytotoxicity against mammalian cells.

4 barriers and deliver virulence factors into mammalian cells.

5 a protein to recognize membrane aberrancy in mammalian cells.

6 in the establishment of nuclear topology in mammalian cells.

7 e only 10-20 nm thick and closely stacked in mammalian cells.

8 comparative analysis in bacteria, yeast, and mammalian cells.

9 its implication in the study of autophagy in mammalian cells.

10 trol the cholesterol content of membranes in mammalian cells.

11 y to profile their ubiquitination targets in mammalian cells.

12 er codon suppression in Escherichia coli and mammalian cells.

13 cryo-CLEM) of virus-infected or transfected mammalian cells.

14 hyladenine (6mA), has recently been found in mammalian cells.

15 aster regulator of defense against stress in mammalian cells.

16 factor-beta (TGF-beta) signaling pathway in mammalian cells.

17 e of E to increase the production of VLPs in mammalian cells.

18 xploited for label-free metabolic imaging of mammalian cells.

19 press and localize to the plasma membrane of mammalian cells.

20 ability to penetrate the outer membranes of mammalian cells.

21 t on the early consequences of aneuploidy in mammalian cells.

22 double strand break (DSB) repair pathway in mammalian cells.

23 gG1 antibodies, which were then expressed in mammalian cells.

24 he atypical ERK3/4-MK5 signalling pathway in mammalian cells.

25 the largest and most complicated enzymes in mammalian cells.

26 that degrades glutathione in the cytosol of mammalian cells.

27 CLYBL operates as a citramalyl-CoA lyase in mammalian cells.

28 is an essential constituent of membranes in mammalian cells.

29 ependent response that limits the fitness of mammalian cells.

30 oxidation product 5-hydroxymethylcytosine in mammalian cells.

31 hanism regulating chromatin configuration in mammalian cells.

32 structures along with their dynamics in live mammalian cells.

33 anced uptake, relative to unmodified DNA, in mammalian cells.

34 that is folded and efficiently secreted from mammalian cells.

35 ethered to POs are unknown, in particular in mammalian cells.

36 l required for efficient NOTCH1 signaling in mammalian cells.

37 osition of the symmetric dimethylarginine in mammalian cells.

38 used dCas9-VP64 activator in both plant and mammalian cells.

39 oporin activity elevates cytosolic Ca(2+) in mammalian cells.

40 maging of endogenous RNA molecules in living mammalian cells.

41 photostability of tandem fusion proteins in mammalian cells.

42 vidence that this regulation is conserved in mammalian cells.

43 w concepts that are emerging from studies in mammalian cells.

44 ed genetic instability and transformation in mammalian cells.

45 sh embryos and in both primary and malignant mammalian cells.

46 larity and cell-cell adhesion machineries in mammalian cells.

47 nal aptamer that labels cell-surface EGFR on mammalian cells.

48 r) or other proteins can be transported into mammalian cells.

49 es a novel tool to manipulate cholesterol in mammalian cells.

50 mmon posttranscriptional RNA modification in mammalian cells.

51 ix) during mesenchymal lineage commitment in mammalian cells.

52 e that is approximately 60 times faster than mammalian cells.

53 cule-dependent transcriptional activation in mammalian cells.

54 required for biosynthesis of sialic acid in mammalian cells.

55 l sorting for perfusion culture of suspended mammalian cells.

56 at facilitates receptor-mediated uptake into mammalian cells.

57 as facilitated forward genetic screenings in mammalian cells.

58 infectious virus following transfection into mammalian cells.

59 id implementation of inducible CRISPR-TRs in mammalian cells.

60 sible for most of the protein degradation in mammalian cells.

61 e study of cellular and molecular biology in mammalian cells.

62 om polypeptides to single cell organisms and mammalian cells.

63 ree analysis of metabolic data applicable to mammalian cells.

64 subset of primary microRNAs (pri-miRNAs) in mammalian cells.

65 d inducer or repressor of gene expression in mammalian cells.

66 the formation of ER-mitochondria contacts in mammalian cells.

67 ks and elevates sister chromatid exchange in mammalian cells.

68 when exposed to the reducing environment in mammalian cells.

69 adhesion-based biomarkers for characterizing mammalian cells.

70 n factor regulates the response to stress in mammalian cells.

71 of cholesterol, and peroxisome biogenesis in mammalian cells.

72 nergy homeostasis, and glucose metabolism in mammalian cells.

73 ization of miRNP components in Mfn2-depleted mammalian cells.

74 vidence that LDs are dispensable for ERAD in mammalian cells.

75 mplexes were nontoxic to either bacterial or mammalian cells.

76 ncided at sites where R-loops accumulated in mammalian cells.

77 rmin 2 (INF2) stimulates Drp1 recruitment in mammalian cells.

78 light-dependent control of transcription in mammalian cells.

79 of a full-length functional human GLP-1R in mammalian cells.

80 cell-to-cell variation of gene expression in mammalian cells.

81 nase acting on RNA type 2) to transcripts in mammalian cells.

82 ine protein kinase ubiquitously expressed in mammalian cells.

83 d this regulation is conserved from yeast to mammalian cells.

84 he AMPs exhibited inhibited toxicity against mammalian cells.

85 eading of repressive chromatin in interphase mammalian cells.

86 hagy and host defense genes in nematodes and mammalian cells.

87 In response to DNA damage mammalian cells activate a complex network of stress res

88 In mammalian cells, active or primed enhancers are commonly

89 Mammalian cell adhesion to the extracellular matrix infl

90 Consistent with the above, Crb3 knockdown in mammalian cells affects the dynamics of IFT particle mov

91 at plays an important role in the defense of mammalian cells against influenza A viruses.

92 Manipulating PKC activity in cultured mammalian cells alters nuclear size, as does expression

93 have been shown to undergo transfer between mammalian cells, although the mechanism behind this phen

94 properties, but many of them are inactive in mammalian cells and are thus precluded from genome-editi

95 ligase: holocarboxylase synthetase (HCS) in mammalian cells and BirA in microbes.

96 compartments of the endolysosomal system in mammalian cells and discuss the mechanisms that spatiote

97 is required for Parkin-induced mitophagy in mammalian cells and for the clearance of paternal mitoch

98 t GrpEL1 has a role as a stress modulator in mammalian cells and highlight that multiple NEFs are inv

99 ally focusing on probes developed for use in mammalian cells and in vivo.

100 tion enhanced viral productivity in infected mammalian cells and induced stronger host immune and inf

101 -containing proteins in Escherichia coli and mammalian cells and is believed to result from post-tran

102 catalyzed by fucosyltransferase 8 (FUT8) in mammalian cells and is involved in various biological fu

103 cable to mechanistic studies of chromatin in mammalian cells and is particularly suited to the analys

104 inhibits mitochondrial respiration in intact mammalian cells and live animals.

105 anism for establishing PO-ER associations in mammalian cells and report a new function for ACBD5 in P

106 manates from the surface of most postmitotic mammalian cells and serves as a sensory organelle, trans

107 regulator of ribosomal protein production in mammalian cells and suggest that this activity can be co

108 is pathway, including conserved responses in mammalian cells and surprising similarities with mechani

109 a as a flexible platform for studying RNA in mammalian cells and therapeutic development.

110 ed CRISPR array, we edit up to four genes in mammalian cells and three in the mouse brain, simultaneo

111 s of internalization of the particles by the mammalian cells and to distinguish their extra- vs intra

112 between human OPTN and ANG were validated in mammalian cells and zebrafish, MAP2K5 kinase emerged as

113 c/apyrimidinic (AP) endonuclease activity in mammalian cells, and a key factor in base-excision repai

114 ficant selectivity for killing bacteria over mammalian cells, and finally, why development of resista

115 mbly of a tetrahedral structure in bacteria, mammalian cells, and mice without evidence of inflammati

116 egan with enzymology and the use of cultured mammalian cells, and progressed to utilize the technique

117 TDP-43-mediated impairments are conserved in mammalian cells, and, importantly, the human ortholog CH

118 ough this protocol focuses on EM in cultured mammalian cells, APEX2 is applicable to many cell types

119 ortation of drug in the plasma membrane of a mammalian cell are the prerequisite for its function on

120 ely, variant library expression platforms in mammalian cells are far from ideal, hindering the study

121 KEY POINTS: Mammalian cells are frequently exposed to stressors caus

122 Mammalian cells are surrounded by diverse nutrients, suc

123 These tests, especially mammalian cell assays, have high sensitivity but suffer

124 We validate mitoxantrone in orthogonal mammalian cell-based assays, demonstrating that our scre

125 anced yeast two-hybrid (HT-eY2H) assay and a mammalian-cell-based Gaussia princeps luciferase protein

126 Cholesterol is an essential compound in mammalian cells because it is involved in a wide range o

127 We show that, in primary mammalian cells, BRCA1, but not BRCA2, suppresses the fo

128 In mammalian cells, bulky DNA adducts located in the templa

129 have been the subject of intensive study in mammalian cells but less is known about effects of splic

130 We demonstrate that conditioning of mammalian cells by electroporation with nanosecond pulse

131 AAA ATPase, regulates spindle orientation in mammalian cells by limiting the levels of cortical NuMA.

132 tically regulate gene expression programs in mammalian cells by modulating the stability and translat

133 Cbl enters mammalian cells by receptor-mediated endocytosis of prot

134 Ap4A is mainly produced in the mammalian cells by S207-phosphorylated Lysyl-tRNA synthe

135 precede the IDD or beta-strands, whereas in mammalian cells, C-terminally located alpha-helical doma

136 Some RNAs in mammalian cells can help to silence the DNA they are tra

137 Yet, mammalian cells carrying Rb deletions are still able to

138 thaliana HAP2/GCS1 is sufficient to promote mammalian cell-cell fusion.

139 hway and that knockdown of Rngtt in cultured mammalian cells compromised Shh pathway activity, sugges

140 However, the detailed mechanism of how mammalian cells cope with the replication stress at thes

141 tion of magnetic nanoparticle synthesis into mammalian cells creates a real and compelling, cytocompa

142 ssue culture room by personnel with standard mammalian cell culture expertise.

143 Mammalian cell culture expressions enable the sufficient

144 In nematodes and mammalian cell culture models of diverse lysosomal disor

145 purified at industrial scale with a standard mammalian cell culture platform and a routine purificati

146 ombination of biochemical reconstitution and mammalian cell culture, we elucidate a mechanism by whic

147 mined on two important pathway families, the mammalian cell-cycle and a set of p53-related pathways,

148 liabilities included those that investigated mammalian cell cytotoxicity, C. parvum proliferation inh

149 avage of concatemeric DNA is not required in mammalian cell DNA replication, indicating that drugs ta

150 We report that members of the mammalian cell entry (MCE) protein family form hexameric

151 (MAM(HS)), which contains an array of seven mammalian cell entry domains.

152 cterium smegmatis contains 6 homologous mce (mammalian cell entry) operons which have been proposed t

153 were first described as being important for Mammalian Cell Entry.

154 ) metabolism at the trans-Golgi membranes in mammalian cells essentially controls the structural feat

155 of miRNA expression and promoters in primary mammalian cells, establishing a foundation for detailed

156 E3 as ribonucleoprotein (RNP) complexes into mammalian cells, establishing DNA-free base editing.

157 rial strains increased viral co-infection of mammalian cells even at a low virus-to-host cell ratio.

158 use of 10 mug of DNA isolated from cultured mammalian cells exposed to a model pyridyloxobutylating

159 r potential genotoxicity and cytotoxicity in mammalian cells exposed to disinfected wastewaters.

160 in naked DNA and in genomic DNA of cultured mammalian cells exposed with NNKOAc.

161 eloped a synthetic biology platform based on mammalian cells expressing the human Fat4 and Ds1.

162 uction, however, the high production cost of mammalian cell expression impedes patient access to such

163 In mammalian cells, expression of mutant M1 spastins, but n

164 echnique, we observe that the mass of living mammalian cells fluctuates intrinsically by around one t

165 imilar to past work in vesicles derived from mammalian cells, fluctuating domains are observed in ZF4

166 f proteins entering the secretory pathway in mammalian cells frequently requires the insertion of dis

167 tablish a system that begins to reconstitute mammalian cell fusion.

168 two-component programme for the induction of mammalian cell fusion.

169 ignificance of external glutamine supply for mammalian cell growth and proliferation.

170 encode a pertussis-like toxin that inhibits mammalian cell growth in vitro We found that this protei

171 Electrical lysis of mammalian cells has been a preferred method in microflui

172 he application of RNA interference (RNAi) to mammalian cells has provided the means to perform phenot

173 Hence, we have shown for the first time that mammalian cells have a pathway for transferring reducing

174 We found that mammalian cells have a strong tendency to maintain physi

175 Mammalian cells have evolved a common DNA damage respons

176 Furthermore, in both Drosophila and mammalian cells, heterochromatin domains exhibit dynamic

177 In mammalian cells, histone deacetylase (HDAC) and Sirtuin

178 In mammalian cells, IMPDH filaments can associate into micr

179 el expression of the human ClC-Kb channel in mammalian cells impedes the functional study of CLCNKB m

180 ory regions of inducible genes in interphase mammalian cells, implicating mitosis-independent functio

181 tubulin with silicon rhodamine (SiR) in live mammalian cells in an efficient and robust manner.

182 rify HA-tagged mitochondria from homogenized mammalian cells in approximately 12 min.

183 f attention since its first reported uses in mammalian cells in early 2013 due to its perceived impac

184 ures that form on the dorsal surface of many mammalian cells in response to growth factor stimulation

185 carbons and amino acid-derived ammonia, most mammalian cells in tissue culture cannot proliferate or

186 reported to be the brightest in non-neuronal mammalian cells, in primary neuronal culture, in brain s

187 s and extends the bivalency model posited in mammalian cells, in which the coexistence of H3K4me3 and

188 -activated chloride channel expressed widely mammalian cells, including epithelia, vascular smooth mu

189 ct of Hcy on molecular clearance pathways in mammalian cells, including in vitro cultured induced plu

190 oximately 15 muM) recombinantly expressed in mammalian cells, induced a positive shift in the voltage

191 r data suggest that ZIKV, when produced from mammalian cells, infects fetal endothelial cells much mo

192 The senescence of mammalian cells is characterized by a proliferative arre

193 , whereas a novel O-mannosylation process in mammalian cells is predicted to serve the large cadherin

194 physiological function in mammals and adult mammalian cells is still unknown.

195 The primary enzyme that repairs AP sites in mammalian cells is the AP endonuclease (APE1), which fun

196 A key regulator of copper homeostasis in mammalian cells is the copper-transporting P-type ATPase

197 Ubiquitously expressed in mammalian cells, its loss-of-function mutations are the

198 of purified in vitro generated circRNA into mammalian cells led to potent induction of innate immuni

199 Expression of the dCas9-sMTase in mammalian cells led to predictable and efficient (up to

200 HS and LS alpha4beta2 nAChRs expressed in a mammalian cell line.

201 bodies (mAbs) manufactured from immortalized mammalian cell lines are becoming increasingly important

202 enotyping variant libraries in transfectable mammalian cell lines in two steps.

203 exemplified by 1, where cytotoxicity against mammalian cell lines was reduced, solubility and plasma-

204 ons utilizing Caenorhabditis elegans models, mammalian cell lines, primary neurons and mouse brains,

205 ion of filovirus glycoproteins in insect and mammalian cell lines.

206 and 700 Hz to individual nuclei of multiple mammalian cell-lines that were compressed between two pl

207 vely characterize full-length otoferlin from mammalian cell lysate.

208 In mammalian cells, M1 coimmunoprecipitated with Apaf-1-pro

209 e observed severe effects of microgravity on mammalian cells, many astronauts have completed long ter

210 y atypical glycomic profile of the cancerous mammalian cell membrane and successfully made a distinct

211 Mammalian cell membranes have different phospholipid com

212 r 30% of the effectors localize to yeast and mammalian cell membranes, including a subset of previous

213 ipid interactions on dynamic organization in mammalian cell membranes, we have performed coarse-grain

214 d compositions that mimic both bacterial and mammalian cell membranes.

215 electrochemical energy can be harvested from mammalian cells, more specifically from white blood cell

216 The establishment of planar polarization by mammalian cells necessitates the integration of diverse

217 t study, we show that Salmonella can exploit mammalian cell non-classical microRNA processing machine

218 ombinant expression of antibody molecules in mammalian cells offers important advantages over traditi

219 Engineered genetic circuits for mammalian cells often require extensive fine-tuning to p

220 mulating only in E. coli None accumulated in mammalian cells or heat-killed bacteria, suggesting meta

221 ive mechanism without detectable toxicity to mammalian cells or induction of bacterial resistance.

222 tion) typically require either expression in mammalian cells or purification from immunized mammals.

223 relied on the self-organizing properties of mammalian cells or used bioengineered constructs to arra

224 (s) inserted at a PTC during this process in mammalian cells, or how the surrounding sequence context

225 Here we characterize mammalian cells overexpressing the GATOR1 component NPRL

226 interpretation of stable isotope patterns in mammalian cells owing to the superimposition of differen

227 a sensory appendage that is present in most mammalian cells, plays critical roles in signaling pathw

228 lity and recruitment of beta-III-spectrin in mammalian cells, pointing to a potential disease mechani

229 cribe a straightforward protocol to generate mammalian cell populations with low to nondetectable lev

230 fficulty of detecting RNAi in virus-infected mammalian cells reflects the expression of highly effect

231 has a history of uncovering novel aspects of mammalian cell regulation.

232 Mammalian cells replicate their chromosomes via a tempor

233 Cellular metabolism in mammalian cells represents a challenge for analytical ch

234 we show that damage-induced fork reversal in mammalian cells requires PCNA ubiquitination, UBC13, and

235 Indeed, expression of eVP40 alone in mammalian cells results in the formation and budding of

236 viously known as C2c2) can be engineered for mammalian cell RNA knockdown and binding.

237 ipid droplet (LD), a ubiquitous organelle in mammalian cells, serves as a hub for lipid metabolism.

238 we used fatty acid-azide/alkyne labeling of mammalian cells, showing their transformation into acyl-

239 independent information across a variety of mammalian cell state transitions.

240 omatin state, and gene expression in complex mammalian cell states.

241 ointeractions, in particular, with proteins, mammalian cells/stem cells, and microbials.

242 o-head filament chains highly reminiscent of mammalian cell stress fibers.

243 ctin during clathrin-mediated endocytosis in mammalian cells suggests that the cell controls whether

244 sylation is highly diverse and essential for mammalian cell survival.

245 We show by direct protein delivery into mammalian cells that an HP1alpha mutant incapable of pha

246 insights into the behavior of CRISPR/Cas9 in mammalian cells that could be used for future improvemen

247 ighly specific enzyme system existing in all mammalian cells that is responsible for the detoxificati

248 ke extensions of the plasma membrane of most mammalian cells that serve specialized signaling functio

249 Here, we show in mammalian cells that the ubiquitin ligase ZNF598 is requ

250 In mammalian cells the central precursor for lipid biosynth

251 Critically, in mammalian cells the sensors showed consistent changes in

252 In mammalian cells, the 26S proteasomes vary in composition

253 In mammalian cells, the ER is degraded via a selective auto

254 of U6 and Y RNA 3' ends, suggesting that in mammalian cells, the formation of a 3' end for noncoding

255 rrent retromer model and demonstrate that in mammalian cells, the individual retromer subcomplexes ha

256 support of the stable compartments model in mammalian cells.The different composition of Golgi ciste

257 se a model for telomere length regulation in mammalian cells: The reduced concentration of the shelte

258 In mammalian cells, there are two distinct TRAPP complexes,

259 In mammalian cells, this process is dependent on the recogn

260 y engineering the surfaces of live yeast and mammalian cells through cell surface-initiated controlle

261 he stress granule transcriptome of yeast and mammalian cells through RNA-sequencing (RNA-seq) analysi

262 ur new data with previous publications using mammalian cells/tissues, we propose a high confidence se

263 inesin, can replace Eg5 function, permitting mammalian cells to acquire resistance to Eg5 poisons.

264 from one of the two X chromosomes in female mammalian cells to balance expression dosage between XX

265 Herein, we used animal models and cultured mammalian cells to demonstrate that disruption of the me

266 avenues for the exploration of adaptation of mammalian cells to gravitational changes.

267 simplex virus 1 genome cloned in yeast into mammalian cells to produce infectious virus.

268 s 3D printing of aqueous droplets containing mammalian cells to produce robust, patterned constructs

269 human Cripto-1 and mouse Cryptic produced in mammalian cells to show that these two EGF-CFC homologs

270 e H1 are partially functionally redundant in mammalian cells to suppress RNAP I transcription-associa

271 Using a multiomics approach in mammalian cells treated with four types of mitochondrial

272 ends on clock gene expression cycles in most mammalian cell types.

273 n the mitochondrial matrices of a variety of mammalian cell types.

274 In mammalian cells under one-photon (1P) illumination, RVF5

275 a highly biologically relevant model system, mammalian cells under reversible osmotic stress, and a r

276 The plasma membrane of mammalian cells undergoes constitutive endocytosis, endo

277 Thus, mammalian cells, unlike Saccharomyces cerevisiae or Esch

278 manassantin-induced bioenergetic deficiency, mammalian cells up-regulated aerobic glycolysis, a proce

279 alization of mitochondrial calcium in intact mammalian cells using cryo-scanning transmission electro

280 tracellular mRNA measurements in nonadherent mammalian cells using fluorescence in situ hybridization

281 tools to induce transcription with light in mammalian cells, using the Arabidopsis photoreceptor Fla

282 n proteins that inhibit protein synthesis of mammalian cells via ADP-ribosylation of the eukaryotic e

283 Because betaine can be synthesized in mammalian cells via choline dehydrogenase (CHDH; EC 1.1.

284 Based on the typical composition of a mammalian cell we quantify the energy demand and the OxP

285 g this approach to regulate transcription in mammalian cells, we observed light-dependent redistribut

286 As carmaphycin B is toxic to mammalian cells, we synthesized a series of chemical ana

287 Unlike the other 14 known galectins in mammalian cells, which have dimeric or tandem-repeated C

288 dered by their non-specific toxicity against mammalian cells, which is usually associated with helica

289 ign of AMPs, minimizing the toxicity against mammalian cells while maintaining high antimicrobial act

290 In this study, we demonstrate that akin to mammalian cells, wild-type yeast possess only two TRAPP

291 Treatment of mammalian cells with histone deacetylase inhibitors to i

292 circuits with multiple inputs and outputs in mammalian cells with minimal optimization.

293 ional heterogeneity in the membranes of live mammalian cells with single-molecule sensitivity and app

294 ass B G protein-coupled receptors (GPCRs) in mammalian cells with the incorporation efficiency depend

295 PARK was used to detect 12 different PPIs in mammalian cells, with 5 min temporal resolution and sign

296 ion of sophisticated cellular computation in mammalian cells, with applications in cell and tissue en

297 e late endosomal compartment accumulation in mammalian cells without affecting other organelles (earl

298 ue to degrade endogenous proteins acutely in mammalian cells without prior modification of the genome

299 Studies of cultured mammalian cells, worms, flies, rodents, simians, apes, a

300 re we show that when viruses are produced in mammalian cells, ZIKV, but not the closely related dengu