1 Expression of traS was induced by
mannopine,
although this induction was strongly inhibite
2 Mannopine,
an opine from the mannityl-opine family, is s
3 In contrast, MotA binds both
mannopine and DFG.
4 is of MotA, SocA, and AttC interactions with
mannopine and its DFG precursor.
5 We thus defined one
mannopine and two DFG binding signatures.
6 smid in an operon that directs the uptake of
mannopine,
another opine.
7 MotA is responsible for mannopine import in
mannopine-
assimilating agrobacteria.
8 ain, SocA protein was proposed as a putative
mannopine binding PBP, and AttC protein was annotated as
9 ing PBP, and AttC protein was annotated as a
mannopine binding-like PBP.
10 Our work demonstrates that AttC is not a
mannopine-
binding protein and reveals a specific binding
11 ne agropine is thought to require an enzyme,
mannopine cyclase, coded for by the ags gene located in
12 The PBP MotA is responsible for
mannopine import in mannopine-assimilating agrobacteria.
13 s1, which is responsible for biosynthesis of
mannopine in crown gall tumors.
14 Mannopine inhibited conjugation in a TraS-dependent fash
15 ssion of ags from the lac promoter conferred
mannopine-
lactonizing activity on Escherichia coli.
16 rew well with agropine (AGR) but slowly with
mannopine (
MOP) as the sole carbon source.
17 While Mas1 is required for the synthesis of
mannopine (
MOP) by crown gall tumor cells, MocC is essen
18 Catabolic
mannopine (
MOP) cyclase encoded by certain Agrobacterium
19 in an operon that is inducible by the opine
mannopine (
MOP).
20 Unlike
mannopine-
PBPs, selective DFG-PBPs are present in a wide
21 A 318 bp
mannopine synthase 2' (mas2') promoter element from the
22 criptional activating element affixed to the
mannopine synthase2' (mas2') transcriptional activating
23 e tumors contained an activity that cyclized
mannopine to agropine.
24 is gene is located in an operon coding for a
mannopine transport system and is expressed as part of t
25 Hence,
mannopine would not be imported into nopaline-type agrob