ライフサイエンスコーパス: mannosylation

1 Kdo residue of Kdo2-lipid IVA as the site of mannosylation.

2 the Golgi from the cytoplasm is required for mannosylation.

3 orylceramide due to a defect in sphingolipid mannosylation.

4 cted links to calcium signalling and protein mannosylation.

5 ences between co- and post-translocational O-mannosylation.

6 xtensive hydrogen bonding network, including mannosylation.

7 lycan synthesis by disrupting dystroglycan O-mannosylation.

8 if is known to be a consensus sequence for C-mannosylation.

9 ry amino acid selectivity near the site of O-mannosylation.

10 phosphatidylinositol-anchoring, and C- and O-mannosylation.

11 -branching, core 1 O-glycan formation, and O mannosylation.

12 a1-6-D-myo-inositol-1-HPO4-sn-lipid precedes mannosylation.

13 hown to catalyze the initial step of protein mannosylation.

14 to catalyze the next two sequential alpha1,2-mannosylations.

15 normal brain development requires protein O-mannosylation activity in neural tissue but not the meni

16 vrg4 mutation causes a general impairment in mannosylation, affecting N-linked and O-linked glycoprot

17 O-Mannosylation also increases the thermostability of CBM

18 To investigate the role played by mannosylation, an immunoreactive cryptococcal mannoprote

19 degrees of microheterogeneity; 9 sites of O-mannosylation and 14 sites of O-GalNAcylation were obser

20 yl-PI accumulation would also be expected if mannosylation and acylation were independent of each oth

21 that Mnt1 and Mnt2 are involved in O-linked mannosylation and are required for virulence.

22 iative S(N)2-like mechanism, whereas alpha-O-mannosylation and beta-C-mannosylation are dissociative

23 these unusual sugar modifications, protein O mannosylation and glycan modifications involving the put

24 that the alpha1,6-, alpha1,2-, and alpha1,3-mannosylation and Kex2p-dependent processing events mark

25 as indicated that the alpha1,6- and alpha1,3-mannosylation and Kex2p-dependent processing of pro-alph

26 ein is a significant substrate for protein O-mannosylation and led to the identification of several n

27 O-Mannosylation and N-glycosylation are essential protein

28 ins of fungi are modified by N- and O-linked mannosylation and phosphomannosylation, resulting in cha

29 P1 suggested that it is modified by O-linked mannosylation, and ConA binds to these O-linked mannose

30 s, particularly with respect to sialylation, mannosylation, and fucosylation, in normal, pancreatitis

31 tive glycosyltransferase genes involved in O-mannosylation are associated with a loss of ligand-bindi

32 putative glycosyltransferases involved in O-mannosylation are causal for various forms of congenital

33 sm, whereas alpha-O-mannosylation and beta-C-mannosylation are dissociative and S(N)1-like.

34 Here, the effects of O-mannosylation are examined on the Family 1 CBM from the

35 Additional functions of O-mannosylation are still largely unknown.

36 eldin A-treated cells received only alpha1,6-mannosylation as did approximately 50% of pro-alphaf tra

37 and suggest that in the absence of proper O-mannosylation, as is associated with certain forms of mu

38 athies, result from defects in the protein O-mannosylation biosynthetic pathway.

39 , and CaMnt5 did not participate in O-linked mannosylation, but CaMnt3 and CaMnt5 had redundant activ

40 is acyl-CoA-dependent and takes place before mannosylation, but uniquely for this class of inositol-a

41 Mannosylation by DPY19L1 but not DPY19L3 is required for

42 canopathy syndrome, supported by deficient O-mannosylation by muscle immunohistochemistry.

43 viding proof of principle that yeast-derived mannosylation can enhance immunogenicity.

44 te-directed mutagenesis, and expression in C-mannosylation-defective Chinese hamster ovary cell varia

45 partial loss of CaVRG4 function resulted in mannosylation defects, which in turn led to a number of

46 O-mannosylation-deficient embryos failed to proceed from t

47 The results show that, although CBM mannosylation does not induce major conformational chang

48 olecular and genetic mechanisms of protein O-mannosylation during development.

49 ant part of this glycosylation is a unique O-mannosylation, essential for the interaction of alpha-dy

50 this enzyme actually carries out an alpha1,3-mannosylation, followed by an alpha1,6-mannosylation, to

51 gyl ether in the benzylidene acetal directed mannosylation has a detrimental effect on stereoselectiv

52 In recent years protein O-mannosylation has become a focus of attention as a patho

53 Loss of protein O-mannosylation in both mutant strains was unambiguously d

54 it was suggested that acylation must precede mannosylation in both yeast and rodent cells because Glc

55 To study the roles of O-mannosylation in brain development we generated a condit

56 amily participates in three types of protein mannosylation in C. albicans, and these modifications pl

57 To begin to study the role of Golgi mannosylation in C. albicans, we isolated the CaVRG4 gen

58 monstrate that TSR1 from punctin-1 carries C-mannosylation in close proximity to O-linked fucose.

59 Our study demonstrates that regulation of O-mannosylation in higher eukaryotes is more complex than

60 Our results redefine the significance of O-mannosylation in humans and other mammals, showing the i

61 rprisingly, SL15 also corrects the defective mannosylation in Lec35 cells.

62 cation processes were required for protein O-mannosylation in M. tuberculosis.

63 of the biological significance of protein O-mannosylation in mycobacteria and demonstrate the crucia

64 ding of physiology and biochemistry of Trp N-mannosylation in proteins and the overall biochemical me

65 e earlier suggestion that acylation precedes mannosylation in rodents cells.

66 Here, we reinvestigate protein O-mannosylation in the context of protein translocation.

67 rate that inositol acylation is required for mannosylation in the HeLa cell GPI biosynthetic pathway,

68 e thus analyzed the impact of interrupting O-mannosylation in the nonpathogenic saprophyte Mycobacter

69 hat differentially expressed N- and O-linked mannosylation in the yeast Pichia pastoris and compared

70 However, the functional importance of O-mannosylation in these tissues at later stages remains l

71 o provide N-linked and/or extensive O-linked mannosylation increased the capacity of the model Ag OVA

72 Protein O-mannosylation is a glycan modification that is required

73 eramides [GIPCs]) contains Man and that this mannosylation is affected in gonst1.

74 Protein O-mannosylation is an essential and evolutionarily conserv

75 Protein O-mannosylation is an essential post-translational modific

76 Among them, O-mannosylation is an unusual type of protein glycosylatio

77 e GlcNalpha-acyl-PI accumulates in vivo when mannosylation is blocked.

78 Thus, the process of protein mannosylation is conserved between M. tuberculosis and e

79 Thus, mannosylation is critical for optimal T cell responses t

80 Protein O-mannosylation is found in yeast and metazoans, and a fam

81 dependent upon the POMGnT1 enzyme and that O-mannosylation is not limited solely to alpha-DG in the b

82 e for RP and indicates that proper protein O-mannosylation is not only essential for early organ deve

83 Together, our data suggested that protein O-mannosylation is required for normal sensory feedback to

84 Protein C-mannosylation is the attachment of alpha-mannopyranose t

85 ransport (and hence Golgi apparatus-specific mannosylation) is a fungus-specific process.

86 st to the highly conserved requirement for O mannosylation, more generic O glycans present on alpha-D

87 insights gained from the characterization of mannosylation mutants into the role of these cell wall c

88 erated in vitro by pure LpcC showed that the mannosylation occurs on the inner Kdo residue of Kdo(2)-

89 he presence and functional significance of C-mannosylation of ADAMTS-like 1/punctin-1, which contains

90 usly shown for LCMV, we found that protein O mannosylation of alpha-DG is crucial for the binding of

91 sferase, POMGnT1, which is involved in the O-mannosylation of alpha-dystroglycan.

92 Surprisingly, O-mannosylation of cadherins and protocadherins does not r

93 In contrast, it has been reported that mannosylation of endogenous GlcNalpha-PI by Trypansoma b

94 hese results suggest that a complete lack of mannosylation of glycoproteins in the Golgi leads to inv

95 lly reduces transcription of PIGM and blocks mannosylation of GPI, leading to partial but severe defi

96 Mannosylation of Kdo2-lipid IVA catalyzed by RfaC procee

97 r Lec35p was required only for MPD-dependent mannosylation of LLO and glycosylphosphatidylinositol in

98 +) T cell clone required N-terminal O-linked mannosylation of MPT32 by a mannosyltransferase encoded

99 Here, we present evidence that alpha1,2-mannosylation of pro-alphaf is also initiated in a disti

100 osphatidylinositol membrane anchoring, and O-mannosylation of protein.

101 The substrate for mannosylation of proteins and lipids in the Golgi appara

102 an-P-Dol-mediated reactions in N-, O-, and C-mannosylation of proteins, GPI anchor assembly, and the

103 GDP-mannose is required for O-mannosylation of proteins, including alpha-DG, and it is

104 , consistent with an interaction between the mannosylation of PTP1 and some unknown host cell mannose

105 These data suggest that the O-mannosylation of PTP1 may have functional significance f

106 ed glycoprotein modifications as well as the mannosylation of sphingolipids.

107 in yeast that catalyzes the first step in O mannosylation of target proteins.

108 -12 mannosyl residues followed by additional mannosylation of the core and arabinosylation of a singl

109 e palmitoylation of the inositol residue and mannosylation of the glucosamine residue of the glucosam

110 l-PI(C8) could be mannosylated in vitro, but mannosylation of the latter was significantly more effic

111 its TSR domain, and obtained evidence that C-mannosylation of the TSR influences LPS binding.

112 defects of smp3 mutants and permits in vivo mannosylation of trimannosyl (Man(3))-GPIs.

113 port that provides definitive evidence for N-mannosylation of Trp residues in a protein.

114 reactions were investigated: MPD-dependent C-mannosylation of tryptophanyl residues, and glucose-P-do

115 C-Mannosylation of TSR1 of the related protease ADAMTS5 wa

116 ned out that prolonged ER residence allows O-mannosylation of un-/misfolded proteins or slow folding

117 fect cell wall integrity, changes in surface mannosylation or the provision of additional carbon sour

118 Disruption of the O-mannosylation pathway involved in functional glycosylati

119 lassical and evolutionarily conserved POMT O-mannosylation pathway is essentially dedicated to alpha-

120 tently, mutations in genes involved in the O-mannosylation pathway result in infantile-onset, severe

121 1, which encodes an essential component in O-mannosylation pathway, in three unrelated families with

122 Mannosylation patterns were mimicked by FL Ig-derived si

123 ent knock-out mouse models associated with O-mannosylation (POMGnT1, LARGE (Myd), and DAG1(-/-)).

124 used by mutations in two genes involved in O-mannosylation, POMT1 and POMGnT1, respectively.

125 ne are interpreted as indicating that beta-O-mannosylation proceeds via an associative S(N)2-like mec

126 and a few other proteins, whereas a novel O-mannosylation process in mammalian cells is predicted to

127 A posttranslational protein O-mannosylation process resembling that found in fungi and

128 Since defects in the O-mannosylation protein glycosylation pathway are primaril

129 For the 4,6-O-benzylidene-directed mannosylation reaction a significant difference in conce

130 n the 4,6-O-benzylidene acetal directed beta-mannosylation reaction.

131 tivity of sensory neurons, suggesting that O-mannosylation regulates the sensory feedback controlling

132 Ag mannosylation represents a promising strategy to augment

133 rexpression of LARGE in cells deficient in O mannosylation resulted in highly glycosylated alpha-DG t

134 C-mannosylation sites were identified in TSP1, linker TSP4

135 ich contains four TSRs (two with predicted C-mannosylation sites), using mass spectrometry, metabolic

136 The distribution of identified sites of O-mannosylation suggests a limited role for local primary

137 hed a microsomal translation/translocation/O-mannosylation system.

138 on its apparent molecular mass of 98 kDa and mannosylation, the antigen of interest was named MP98.

139 pathway related to the yeast-type protein O-mannosylation, the enzymatic basis and functional import

140 ha1,3-mannosylation, followed by an alpha1,6-mannosylation, to form the first branched pentasaccharid

141 ing to muscle cell glycoproteins, although O-mannosylation was intact.

142 , the high alpha selectivity observed with C-mannosylation was reversed to high beta selectivity if t

143 two sites of a rare type of glycosylation (C-mannosylation) were identified at tryptophan residues 43

144 O-Mannosylation, which accounts for up to 30% of the repor