ライフサイエンスコーパス: mannosyltransferase

1 1p outerchain alpha1,6-Man branch-initiating mannosyltransferase.

2 covered the new gene (lpcC) that encodes the mannosyltransferase.

3 that strongly resembles Och 1p, an alpha-1,6-mannosyltransferase.

4 cerevisiae, we have used Och1p, a cis-Golgi mannosyltransferase.

5 o observed with the Saccharomyces cerevisiae mannosyltransferase.

6 at the C-terminal domain is an alpha-(1-->3)-mannosyltransferase.

7 the activity of the C-terminal alpha-(1-->3)-mannosyltransferase.

8 lure to maintain residence of a medial Golgi mannosyltransferase.

9 endogenous inositol phosphorylceramide (IPC) mannosyltransferase.

10 is polymerized by the serotype-specific WbdA mannosyltransferase.

11 yl-P-Man: Man(7)GlcNAc(2)-PP-dolichyl-alpha6-mannosyltransferase.

12 ntial for substrate recognition by the first mannosyltransferase.

13 l for the inositol acyltransferase and first mannosyltransferase.

14 motif present in the cytosolic tails of some mannosyltransferases.

15 s of the respective Saccharomyces cerevisiae mannosyltransferases.

16 pha-DG, and it is the substrate of cytosolic mannosyltransferases.

17 by moderate overexpression of several Golgi mannosyltransferases.

18 inhibit many Golgi-located, Mn(2+)-dependent mannosyltransferases.

19 (protein O-mannosyltransferase) family of O-mannosyltransferases.

20 displays distant similarity to yeast protein mannosyltransferases.

21 to dolichol phosphate-D-mannose:protein O-D-mannosyltransferases.

22 Genetic defects in protein O-mannosyltransferase 1 (POMT1) and POMT2 underlie severe

23 of glycosyltransferases, including protein O-mannosyltransferase 1, beta3-N-acetylglucosaminyltransfe

24 ction, and was termed CMT1, for cryptococcal mannosyltransferase 1.

25 complex of mutually indispensable protein O-mannosyltransferases 1 and 2 (POMT1 and 2).

26 Phosphatidyl-myo-inositol mannosyltransferase A (PimA) is an essential glycosyltra

27 Phosphatidyl-myo-inositol mannosyltransferase A (PimA) is an essential glycosyltra

28 Phosphatidyl-myo-inositol mannosyltransferase A (PimA) is an essential glycosyltra

29 that have both been shown to have alpha-1,6-mannosyltransferase activity in vitro.

30 ha1,6-mannosyltransferase but lacks alpha1,2-mannosyltransferase activity in vivo.

31 ysis, we demonstrated that the alpha-(1-->2)-mannosyltransferase activity of the N-terminal domain is

32 minal domain of WbdA possesses alpha-(1-->2)-mannosyltransferase activity, and we demonstrate in this

33 Both protein complexes have alpha-1, 6-mannosyltransferase activity, forming a series of poly-m

34 domain of WbdA(O9a) possesses alpha-(1-->2)-mannosyltransferase activity.

35 monstrated that Ktr3p, a cis-Golgi-localized mannosyltransferase, also relies on Erv26p for efficient

36 y1 displayed a reduced half-life of the Och1 mannosyltransferase, an established cargo of intra-Golgi

37 ed in the cis-Golgi marker enzymes alpha 1,6 mannosyltransferase and GDPase.

38 uced amino acid sequence shows similarity to mannosyltransferases and other glycosyltransferases.

39 he cytosolic domains of cis and medial Golgi mannosyltransferases and that loss of this interaction c

40 a bifunctional alpha-(1-->2)-, alpha-(1-->3)-mannosyltransferase, and its counterpart in serotype O8

41 e, inositol acyltransferase, all four of the mannosyltransferases, and the ethanolamine phosphate tra

42 nd pattern of conservation in the O9a and O8 mannosyltransferases are not consistent with the existin

43 oss-of-function alleles of ALG12, encoding a mannosyltransferase, as suppressors of a temperature-sen

44 dD-interaction site remained, the N-terminal mannosyltransferase became an unrestricted polymerase, c

45 clustered genes of C. glabrata encoding beta-mannosyltransferases, BMT2-BMT6, were deleted simultaneo

46 ly Golgi compartment that houses an alpha1,6-mannosyltransferase but lacks alpha1,2-mannosyltransfera

47 hat the inhibitors bind to the first alpha-D-mannosyltransferase by means of charge interactions with

48 MNN2 is an alpha-1, 2-mannosyltransferase catalyzing the addition of the first

49 ansport was blocked in vivo, subunits of the mannosyltransferase complex accumulated in the vacuole.

50 ervations indicate that the Mnn9p-containing mannosyltransferase complexes cycle back and forth betwe

51 ion of the C. glabrata Anp1, Mnn2, and Mnn11 mannosyltransferases directly affects the structure of t

52 urface-exposed alpha-helix in the C-terminal mannosyltransferase domain of WbdA as the site of intera

53 A single C-mannosyltransferase (dumpy-19, DPY-19), modifying the fi

54 erminal O-linked mannosylation of MPT32 by a mannosyltransferase encoded by the Rv1002c gene.

55 homologous family of four putative protein O-mannosyltransferases encoded by the TMTC1-4 genes, which

56 osition -270 from the start codon of PIGM, a mannosyltransferase-encoding gene, disrupts binding of t

57 telium discoideum which encodes the beta-1,4-mannosyltransferase enzyme that catalyzes the reaction:

58 synthesized as a polyprenol-linked glycan by mannosyltransferase enzymes located at the cytoplasmic m

59 etylase, inositol acyltransferase, and first mannosyltransferase enzymes.

60 athway to the well-described POMT (protein O-mannosyltransferase) family of O-mannosyltransferases.

61 A second mannosyltransferase from C. neoformans membranes adds ma

62 ylation by deleting the initiating alpha-1,6-mannosyltransferase gene from P. pastoris, several combi

63 knowledge, this study is the first to link a mannosyltransferase gene to osteochondrogenesis.

64 g D-mannosamine (ManN) as a tool to identify mannosyltransferase genes involved in LAM synthesis.

65 to mammalian organisms, Drosophila has two O-mannosyltransferase genes, rotated abdomen (rt) and DmPO

66 Our data suggest that the two Drosophila O-mannosyltransferase genes, rt and tw, have nonredundant

67 In vertebrates, a new C-mannosyltransferase has apparently evolved to increase g

68 lcN-(2-O-alkyl)PI weakly inhibited the first mannosyltransferase in a competitive manner.

69 aromyces cerevisiae, the roles of two of the mannosyltransferases in the pathway, Alg2 and Alg11, hav

70 ence that LpqW regulates the activity of key mannosyltransferases in the periplasmic leaflet of the c

71 The family of mammalian O-mannosyltransferases includes two enzymes, POMT1 and POM

72 Thus, MT1671 is the mannosyltransferase involved in deposition of the first

73 the mptA gene, encoding a membrane alpha1-6-mannosyltransferase involved in extending the alpha1-6-m

74 a family of conserved orthologous protein O-mannosyltransferases is believed to initiate this import

75 nservation between yeast and human protein O-mannosyltransferases is uncharacterized.

76 DID), features that are common to eukaryotic mannosyltransferases (ManTs) of the GT-C superfamily tha

77 iple membrane-associated, substrate-specific mannosyltransferases (ManTs) responsible for the sequent

78 ed to characterize the GDP-mannose-dependent mannosyltransferase MgtA from C. glutamicum that extends

79 The yeast alpha-1,3-mannosyltransferase (Mnn1p) is localized to the Golgi by

80 ogether, these studies have identified a new mannosyltransferase, MptA, and they shed further light o

81 acid substitution within the ppMan-dependent mannosyltransferase MptB could bypass the need for LpqW.

82 ional cbk1 mutants mislocalize the cis-Golgi mannosyltransferase Och1, are hypersensitive to the amin

83 Two Golgi mannosyltransferases, Och1p and Mnn1p, were mislocalized

84 The natural substrate for the first alpha-D-mannosyltransferase of glycosylphosphatidylinositol bios

85 d with GlcNalpha-PI(C8), confirming that the mannosyltransferase of trypanosomes is divergent from th

86 ase reactions, and a homology model with the mannosyltransferase PimA, from Mycobacteria smegmatis ,

87 e show for the first time that although both mannosyltransferases PimA and PimB' (MSMEG_4253) recogni

88 In analogy, it was assumed that protein O-mannosyltransferases (PMTs) also act at the translocon,

89 synthesis, including the two human protein O-mannosyltransferases, POMT1 and POMT2, underlie a subgro

90 dystrophies.SIGNIFICANCE STATEMENT Protein O-mannosyltransferases (POMTs) are evolutionarily conserve

91 from dolichyl phosphoryl mannose:polypeptide mannosyltransferase (protein mannosyl transferase; PMT),

92 Golgi-bound mannosyltransferases require Mn(2+) as an essential cofa

93 that HOC1 encodes a Golgi-localized putative mannosyltransferase required for the proper construction

94 Thus, MSMEG4245 is apparently a key mannosyltransferase, required for the proper elongation

95 steady-state Golgi localization of multiple mannosyltransferases requires recognition of their cytos

96 nked glycosylation 2 (Alg2), which encodes a mannosyltransferase residing on the endoplasmic reticulu

97 This is in agreement with the mannosyltransferase role predicted for WadC and the lack

98 Previously mutations in a putative protein O-mannosyltransferase (SCO3154, Pmt) and a polyprenol phos

99 Our data indicate that hSmp3p functions as a mannosyltransferase that adds a fourth mannose to certai

100 MNN1 is an alpha-1,3-mannosyltransferase that adds the terminal mannose to th

101 ALG2 is an alpha-1,3-mannosyltransferase that also catalyses early steps in t

102 an PIG-M, an endoplasmic reticulum-localized mannosyltransferase that is required for synthesis of th

103 A mannosyltransferase that uses GDP-mannose and the conser

104 doplasmic reticulum by a family of protein O-mannosyltransferases that are conserved from yeast (PMTs

105 MNN10 and MNN11 encode mannosyltransferases that are part of the N-glycosylatio

106 ormational transitions are important for the mannosyltransferase to interact with the donor and accep

107 MSMEG_4241 mutant that lacks the alpha-(1,6)-mannosyltransferase used late in LM elongation, we showe

108 s of this synthetic lethality included three mannosyltransferases, VAN1, KTR4, and HOC1.

109 mbrane protein homolog of eukaryotic protein mannosyltransferases, was shown to catalyze the initial

110 ases, WbdC and WbdB, and a serotype-specific mannosyltransferase, WbdA.

111 nol pyrophosphoryl-GlcpNAc, by two conserved mannosyltransferases, WbdC and WbdB, and a serotype-spec

112 lly and pharmacologically blocking protein O-mannosyltransferases, we found that this posttranslation

113 accharomyces cerevisiae ScAlg2, an alpha-1,2-mannosyltransferase, which functions in the early stages

114 maintains the Golgi localization of several mannosyltransferases, which is subsequently critical for

115 e, we initiated purification of an alpha-1,3-mannosyltransferase with appropriate specificity for a r

116 A novel mannosyltransferase with specificity appropriate for a r

117 lustrate assembly systems exploiting several mannosyltransferases with flexible active sites, arrange