ライフサイエンスコーパス: marinum

1 rulence of Mycobacterium tuberculosis and M. marinum.

2 the virulence of both M. tuberculosis and M. marinum.

3 hat oxyR is not critical for virulence in M. marinum.

4 usively at the actin-polymerizing pole of M. marinum.

5 onin support intracellular replication of M. marinum.

6 he fruit fly Drosophila melanogaster with M. marinum.

7 ible to tuberculosis caused by Mycobacterium marinum.

8 culosis, and 85.9% homology to Mycobacterium marinum.

9 nfection of J774 macrophage-like cells by M. marinum.

10 Cultures of a biopsy of the lesion grew M. marinum.

11 in the nontuberculous pathogen Mycobacterium marinum.

12 dentified immediately upstream of katG in M. marinum.

13 acterium Bacillus subtilis, or Mycobacterium marinum.

14 igosaccharide IV (LOS-IV) from Mycobacterium marinum.

15 are important for bacterial virulence of M. marinum.

16 ical medium, correlates with virulence in M. marinum.

17 ysis of culture filtrates from Mycobacterium marinum.

18 , Mycobacterium bovis BCG, and Mycobacterium marinum.

19 e secreted protein fraction of Mycobacterium marinum.

20 colony-forming units in aged cultures of M. marinum.

21 nits from frogs chronically infected with M. marinum.

22 ns of innate susceptibility to Mycobacterium marinum.

23 a where they are productively infected by M. marinum.

24 affect macrophage infection by Mycobacterium marinum.

25 .46 degrees C (53.69 to 55.23 degrees C); M. marinum, 58.91 degrees C (58.28 to 59.55 degrees C); rap

26 rty-two slowly growing NTM, including 7/7 M. marinum, 7/7 M. kansasii, and 7/11 of other less commonl

27 ial response of neutrophils to Mycobacterium marinum, a close genetic relative of M. tuberculosis use

28 tation in the erp homologue of Mycobacterium marinum, a close genetic relative of M. tuberculosis.

29 ible to tuberculosis caused by Mycobacterium marinum, a close genetic relative of the causative agent

30 ture determination of NAT from Mycobacterium marinum, a close relative of the pathogenic Mycobacteriu

31 Finally, we show that Mycobacterium marinum, a model organism for M. tuberculosis, encounter

32 equences during infection with Mycobacterium marinum, a natural fish pathogen.

33 Mycobacterium marinum, a natural pathogen of fish and frogs and an occ

34 Here we show that Mycobacterium marinum, a natural pathogen of fish and frogs and an occ

35 Mycobacterium marinum, a relatively rapid-growing fish and human patho

36 Mycobacterium marinum, a well-recognized cutaneous pathogen, is usuall

37 acting protein, and Cdc42 does not affect M. marinum actin tail formation, excluding the participatio

38 -binding basic motif in N-WASP eliminates M. marinum actin tail formation.

39 M. marinum AhpC levels detected by immunoblotting, were inc

40 In the environment, M. marinum also interacts with amoebae, which may serve as

41 In Mycobacterium marinum, an established model for ESX-1 secretion in Myc

42 e inactivated the oxyR gene in Mycobacterium marinum, an organism used to model M. tuberculosis patho

43 ind to the oxyR-ahpC promoter region from M. marinum and additional mycobacterial species.

44 recent publication in PNAS reported that M. marinum and M. bovis bacillus Calmette-Guerin produce a

45 to characterize an outbreak of Mycobacterium marinum and other nontuberculous mycobacterial skin and

46 including Saprochaete suaveolens, Geotrichum marinum and Saprochaete gigas were diverging significant

47 no-glycerol, was purified from Mycobacterium marinum and subsequently identified as a 5-O-mycolyl-bet

48 f mariner-based transposon mutagenesis of M. marinum and that M. marinum can be used to study the fun

49 e opportunistic human pathogen Mycobacterium marinum and the characterization of this mutant and its

50 cobacterial pathogenesis using Mycobacterium marinum and the goldfish, Carassius auratus.

51 Interestingly, mel(2) is unique to M. marinum and the M. tuberculosis complex and not present

52 The largest regulon is observed in M. marinum and the smallest in M. abscessus.

53 ely related to M. ulcerans and Mycobacterium marinum, and as further evidence is gathered, it will mo

54 bacterial models, including M. bovis BCG, M. marinum, and M. smegmatis have significantly contributed

55 Mycobacterium ulcerans and Mycobacterium marinum are closely related pathogens which share an aqu

56 te that the levels of ESX-1 substrates in M. marinum are fine-tuned by negative feedback control, lin

57 Mycobacterium tuberculosis and Mycobacterium marinum are thought to exert virulence, in part, through

58 with Salmonella typhimurium or Mycobacterium marinum at earlier stages of development, the innate imm

59 SX-5a deletion mutant in the model system M. marinum background was deficient in the secretion of som

60 mplex protein mixture from the Mycobacterium marinum bacterial secretome.

61 Intracellular M. marinum blocked vacuolar acidification and failed to col

62 etic loci required for ESX-1 secretion in M. marinum but also provide an explanation for the observed

63 hese genes in M. bovis, M. bovis BCG, and M. marinum but not in several other Mycobacterium species,

64 or ESX-5-mediated secretion in Mycobacterium marinum, but for which the role in secretion is not know

65 e in the virulence of M. tuberculosis and M. marinum, but the precise molecular and cellular mechanis

66 olated from the photochromogen Mycobacterium marinum by heterologous complementation of an M. marinum

67 her LOS composition affects the uptake of M. marinum by professional phagocytes.

68 level, M. ulcerans is distinguished from M. marinum by the presence of a virulence plasmid which enc

69 results in iniBAC induction in Mycobacterium marinum By transposon mutagenesis, we identified that th

70 sposon mutagenesis of M. marinum and that M. marinum can be used to study the function of M. tubercul

71 These findings show that M. marinum can escape into the cytoplasm of infected macrop

72 we find that susceptibility to Mycobacterium marinum can result from either inadequate or excessive a

73 M. marinum causes a chronic granulomatous, nonlethal diseas

74 Mycobacterium marinum causes long-term subclinical granulomatous infec

75 M. marinum causes systemic disease in fish but produces loc

76 Mycobacterium marinum causes tuberculosis-like disease in fish and amp

77 ed substrates indicated that M. bovis and M. marinum cell extracts contain PLC and PLD activities, bu

78 ope, we found that the majority of viable M. marinum cells were in nonacidic vacuoles that did not co

79 This method has enabled us to isolate 12 M. marinum clones that contain promoter constructs differen

80 nts against 60 recent clinical strains of M. marinum collected from 10 geographic sites within the Un

81 However, we were also able to show that M. marinum contains an even larger set of host-specific vir

82 Therefore, M. marinum contains different sets of virulence factors tha

83 num by heterologous complementation of an M. marinum cosmid library in the nonchromogen Mycobacterium

84 We confirm the previous finding that M. marinum DeltaRD1 mutants are attenuated in adult zebrafi

85 Here we show that M. marinum DeltasecA2 was attenuated for virulence in both

86 M. marinum DeltasecA2 was more sensitive to SDS and had uni

87 We describe a case of Mycobacterium marinum demonstrating robust cord formation.

88 omMycobacterium tuberculosisandMycobacterium marinum Determination of the structures of two complexes

89 Inactivation of oxyR in M. marinum did not affect either virulence in a fish infect

90 we describe a laboratory animal model for M. marinum disease in the leopard frog (Rana pipiens), a na

91 ge infection, we conducted a screen of an M. marinum DNA library that provides 2.6-fold coverage of t

92 Interestingly, M. marinum enters fish monocytes at a 40- to 60-fold-higher

93 ude that ESX-1 plays an essential role in M. marinum escape from the MCV.

94 cing pores in MCV membranes, facilitating M. marinum escape from the vacuole and cell-to-cell spread.

95 e results suggest that ESAT-6 secreted by M. marinum ESX-1 could play a direct role in producing pore

96 In this study, we have examined nine M. marinum ESX-1 mutants and the wild type by using fluores

97 on macrophages: macrophages infected with M. marinum-expressing PGL-1 also damage axons.

98 Mycobacterium marinum, found commonly in salt water and freshwater, is

99 similarities between M. tuberculosis and M. marinum genes in this region that we designate extRD1 (e

100 ted a library of 200-1000 bp fragments of M. marinum genomic DNA inserted upstream of a promoterless

101 by other methods, 9 were PCR positive for M. marinum group species, 8 were IHC positive, and 3 were p

102 Mycobacterium marinum grows at an optimal temperature of 33 degrees C,

103 We demonstrate here that M. marinum grows within Dictyostelium discoideum cells, all

104 M. marinum has become an important model system for the stu

105 An unusual clade of M. marinum has been reported from fish in the Red and Medit

106 Mycobacterium marinum has recently been used as a model for aspects of

107 regulation of M. tuberculosis genes whose M. marinum homologs are induced in chronically infected fro

108 Disruption of the M. marinum homologue of Rv3881c, not previously implicated

109 erminants of susceptibility to Mycobacterium marinum identified a hypersusceptible mutant deficient i

110 pattern and that the LOS pathway used by M. marinum in macrophages is conserved during infection of

111 lna, Sweden) to susceptibility testing of M. marinum in order to assess the activities of eight antim

112 ESX-5a is important for the virulence of M. marinum in the zebrafish model.

113 cells as well as the effects on growth of M. marinum in these cells.

114 However, analysis of Mycobacterium marinum in zebrafish has shown that the early granuloma

115 g M. avium, M. fortuitum, M. gordonae, or M. marinum incubated with various concentrations of ciprofl

116 for the large-scale longitudinal study of M. marinum-induced tuberculosis in adult zebrafish where bo

117 We provide evidence that M. marinum induces membrane pores approximately 4.5 nm in d

118 that metabolism is profoundly affected in M. marinum-infected flies.

119 Mycobacterium marinum-infected zebrafish are used to study tuberculosi

120 Here, we deploy Mycobacterium marinum-infected zebrafish larvae for in vivo characteri

121 We monitored transparent Mycobacterium marinum-infected zebrafish live to conduct a stepwise di

122 We report a case of cutaneous Mycobacterium marinum infection in a renal transplant recipient.

123 e pathogenesis associated with Mycobacterium marinum infection in the fly.

124 responses in vivo, we studied Mycobacterium marinum infection in two different hosts: an established

125 zebrafish embryos to image the events of M. marinum infection in vivo.

126 e previously developed a zebrafish embryo-M. marinum infection model to study host-pathogen interacti

127 ous granuloma in the zebrafish-Mycobacterium marinum infection model, which is characterized by organ

128 cobacteria growth, we examined Mycobacterium marinum infection of Drosophila S2 cells.

129 Consequently, M. marinum infection of mammals is restricted largely to th

130 ) using the zebrafish model of Mycobacterium marinum infection provides new insights into the role of

131 We examined organs of frogs with chronic M. marinum infection using transmission electron microscopy

132 mutant zebrafish are hypersusceptible to M. marinum infection, demonstrating that the control of fis

133 ing protein, IipA, in the pathogenesis of M. marinum infection.

134 on is systemically reduced as a result of M. marinum infection.

135 e empiric drug selection for contemporary M. marinum infections and also provide evidence that the Et

136 rafish embryo infection model that allows M. marinum infections to be visualized in real-time, compar

137 used to identify cases in an outbreak of M. marinum infections.

138 We also found that M. marinum inhibits lysosomal fusion in fish monocytes, ind

139 M. marinum initially proliferated inside the phagocytes of

140 he co-dependent secretion is required for M. marinum intracellular growth in macrophages, where the M

141 Mycobacterium marinum is a pathogenic mycobacterial species that is cl

142 Mycobacterium marinum is a waterborne pathogen responsible for tubercu

143 Virulent M. marinum is able to escape from the Mycobacterium-contain

144 reconstituting these cells, we find that M. marinum is able to use either WASP or N-WASP to induce a

145 Mycobacterium marinum is an established model for discovering genes in

146 M. marinum is closely related to M. tuberculosis, which cau

147 Mycobacterium marinum is closely related to Mycobacterium tuberculosis

148 M. marinum is closely related to the Mycobacterium tubercul

149 fibroblasts lacking both WASP and N-WASP, M. marinum is incapable of efficient actin polymerization a

150 Actin tail formation by M. marinum is markedly reduced in macrophages deficient in

151 ude that the MMAR_0039 gene in Mycobacterium marinum is required to promote Esx-1 export.

152 d cathepsin D comparable to those for the M. marinum isolate.

153 nstrate the best in vitro potency against M. marinum isolates to be as follows (rank order): trimetho

154 opulation of vesicles that contained live M. marinum labeled with the lysosomal glycoprotein LAMP-1,

155 M. marinum lacking the mag24 gene were less virulent, as de

156 We conclude that M. marinum, like M. tuberculosis, can circumvent the host e

157 Mycobacterium marinum, like Mycobacterium tuberculosis, is a slow-grow

158 We have found a Mycobacterium marinum locus of two genes that is required for both inv

159 We too find ESX-1 of M. tuberculosis and M. marinum lyses host cell membranes.

160 n whole-cell extracts of M. tuberculosis, M. marinum, M. bovis, and M. bovis BCG, but this activity w

161 M. fortuitum, M. marinum, M. scrofulaceum, M. avium, and M. chelonae grew

162 We recently constructed a Mycobacterium marinum mel2 locus mutant, which is known to affect macr

163 These observations demonstrate that the M. marinum mel2 locus plays a role in resistance to ROS and

164 Furthermore, the M. marinum melF mutant displays a defect at late stages in

165 ical and infection assays showed that the M. marinum mimG mutant, an Rv3242c orthologue in a pathogen

166 activity was assessed against Mycobacterium marinum (Mm) (a model for Mtb), Pseudomonas aeruginosa (

167 esxA/esxB knockout strains of Mycobacterium marinum (Mm) and Mtb.

168 nd the second vector tested in Mycobacterium marinum (Mm).

169 Here, using the zebrafish-Mycobacterium marinum model, we found that mycobacterial granuloma for

170 In order to advance the utility of the M. marinum model, we have developed efficient transposon mu

171 Within 1 day of injection of Mycobacterium marinum, MsNramp expression was highly induced (17-fold

172 We found that an M. marinum mutant with mutation of the first gene in the me

173 M. marinum mutants in genes homologous to Rv3866-Rv3868 als

174 The M. marinum mutants showed decreased virulence in vivo and f

175 sed to identify the loci responsible, and M. marinum mutants were constructed in the genes involved.

176 M. marinum mutants with mutations in mel(1) and mel(2), con

177 Mycobacterium marinum mutants with transposon insertions in the beta-k

178 homologues complemented the corresponding M. marinum mutants, emphasizing the functional similarities

179 In Drosophila infected with Mycobacterium marinum, mycobacterium-induced STAT activity triggered b

180 contrast to M. ulcerans and conventional M. marinum, mycolactone F-producing mycobacteria are incapa

181 We have also determined the structure of M. marinum NAT in complex with CoA, shedding the first ligh

182 ly, the principal CoA recognition site in M. marinum NAT is located some 30 A from the site of CoA re

183 Depending on the dose of M. marinum organisms administered, an acute or chronic dise

184 with doses between 10(2) and 10(9) CFU of M. marinum organisms.

185 ntified genes expressed specifically when M. marinum persists within granulomas.

186 M. tuberculosis (and M. marinum) PGL promotes bacterial spread to growth-permiss

187 We characterized the Mycobacterium marinum phagosome by using a variety of endocytic marker

188 its close pathogenic relative Mycobacterium marinum, preferentially recruit and infect permissive ma

189 cytic cells from fish, a natural host for M. marinum, provide an extremely valuable model for the ide

190 in enhanced intracellular replication of M. marinum relative to the control wild-type strain.

191 tified 22 gene products from the wildtype M. marinum secretome in a single CZE-tandem mass spectromet

192 In this study, we have examined the M. marinum secretomes and identified four proteins specific

193 tank water, in which case infection with M. marinum should be considered.

194 biopsy can lead to improved diagnosis of M. marinum SSTIs compared to relying solely on mycobacteria

195 An M. marinum strain bearing a transposon-insertion between th

196 t an infection caused by a drug-resistant M. marinum strain in an otherwise healthy patient.

197 zebrafish are exquisitely susceptible to M. marinum strain M.

198 We identified a noncytotoxic M. marinum strain with a transposon insertion in a predicte

199 the loss of WhiB6 resulted in a virulent M. marinum strain with reduced ESX-1 secretion.

200 nt, an Rv3242c orthologue in a pathogenic M. marinum strain, was strongly attenuated in adult zebrafi

201 Mutation of two PE-PGRS genes produced M. marinum strains incapable of replication in macrophages

202 decreased, and WhiB6 was not detected in M. marinum strains lacking genes encoding ESX-1 components.

203 pe and the complemented DeltamimG:Rv3242c M. marinum strains showed prominent pathological features,

204 in was only marginally active against the M. marinum strains tested (MIC90, at the National Committee

205 d a genetic screen to identify Mycobacterium marinum strains which failed to lyse amoebae.

206 Together, these data demonstrate that M. marinum subversion of host actin polymerization is most

207 ila melanogaster infected with Mycobacterium marinum suffer metabolic wasting similar to that seen in

208 ork in zebrafish infected with Mycobacterium marinum suggests that granulomas contribute to early bac

209 that this gene plays additional roles in M. marinum survival in the host.

210 In addition, M. marinum survives and replicates in fish monocytes while

211 el for the three-dimensional structure of M. marinum TesA (TesAmm) and demonstrate that a Ser-to-Ala

212 subset of proteins in M. tuberculosis and M. marinum that are important for bacterial virulence of M.

213 M. marinum that polymerized actin were free in the cytoplas

214 the gene expression profile of Mycobacterium marinum, the cause of fish and amphibian tuberculosis, d

215 preferentially expressed when Mycobacterium marinum, the cause of fish and amphibian tuberculosis, r

216 results, 11 of 27 (41%) were positive for M. marinum; the remainder showed no growth.

217 flandii, and the fish pathogen Mycobacterium marinum; the structural diversity in the mycolactone cla

218 d mel(2) are important for the ability of M. marinum to infect host cells.

219 ified two loci that affect the ability of M. marinum to infect macrophages, designated mel(1) and mel

220 vis Bacille Calmette-Guerin or Mycobacterium marinum to thiacetazone, a second line antitubercular dr

221 show here that superinfecting Mycobacterium marinum traffic rapidly into preexisting granulomas, inc

222 have shown that superinfecting Mycobacterium marinum traffic rapidly to established fish and frog gra

223 A screen of Mycobacterium marinum transposon mutant library led to isolation of ei

224 PE_PGRS proteins by screening Mycobacterium marinum transposon mutants for secretion defects.

225 show that flies infected with Mycobacterium marinum undergo a process like wasting: They progressive

226 eloped an in vitro model for the study of M. marinum virulence mechanisms using the carp monocytic ce

227 ylic acid reductase (CAR) from Mycobacterium marinum was found to convert a wide range of aliphatic f

228 erp-deficient M. marinum was growth attenuated in cultured macrophage mon

229 novel locus required for ESX-1 export in M. marinum was identified outside the RD1 locus.

230 M. marinum was isolated from his bone marrow.

231 ence for cell-to-cell spread by wild-type M. marinum was obtained by microscopic detection in macroph

232 Intracellular growth of M. marinum was shown to mimic the properties previously obs

233 um tuberculosis and Mycobacterium leprae, M. marinum was shown to possess a closely linked and diverg

234 insertion mutant (cpsA::Tn) of Mycobacterium marinum was studied.

235 Four reference strains of M. marinum were tested on five occasions with eight drugs (

236 for the intracellular pathogen Mycobacterium marinum whether it uses conserved strategies to exploit

237 levels of ESX-1 substrates in Mycobacterium marinum WhiB6 is a transcription factor that regulates e

238 ped a cutaneous infection with Mycobacterium marinum, which apparently resolved following local heat

239 They also suggest that M. marinum will be useful as a model system for studying th

240 be involved in the early interactions of M. marinum with macrophages.

241 Using Mycobacterium marinum-zebrafish and the surrogate MsmRv3242c infection

242 Using the Mycobacterium marinum-zebrafish model, Cronan et al. (2016) now show t