ライフサイエンスコーパス: matrilysin

1 so target, Matrix metalloproteinase-7 (MMP-7/Matrilysin).

2 ursors by matrix metalloproteinase-7 (MMP-7; matrilysin).

3 r two MMPs, MMP-3 (stromelysin-1) and MMP-7 (matrilysin).

4 r epithelia in the prostate also overexpress matrilysin.

5 thus be exploited to yield metallohybrids of matrilysin.

6 es that are more similar to stromelysin than matrilysin.

7 arterectomy tissue in organ culture released matrilysin.

8 ith Leu in subsite P1' relative to wild type matrilysin.

9 MAT:A215V are similar to those of wild type matrilysin.

10 TIMP-1 inhibits re-epithelialization through matrilysin.

11 ctors enhance the activation and activity of matrilysin.

12 r pocket found in fibroblast collagenase and matrilysin.

13 ecific cleavage of the beta(4) integrin with matrilysin.

14 Matrilysin-1 (also called matrix metalloproteinase-7) is

15 -1 null mice and was correlated with reduced matrilysin-1 expression in BOA.

16 phospho-p38 and phospho-Erk1/2 kinases after matrilysin-1 expression.

17 Forced expression of the matrilysin-1 gene in immortalized human normal airway ep

18 strate that there is increased expression of matrilysin-1 in human lesions and BOA in the CC10-human

19 We conclude that matrilysin-1 may play an important role in the bronchiol

20 haete-scute homolog-1 mice were crossed with matrilysin-1 null mice and was correlated with reduced m

21 In addition, matrilysin-1 stimulated proliferation and inhibited Fas-

22 d HPLD1 cells, which do not normally express matrilysin-1, promoted cellular migration, suggesting a

23 Endometase (matrilysin 2 or MMP-26) is a putative early biomarker fo

24 trix metalloproteinase-26 (MMP-26/endometase/matrilysin-2) is a newly identified MMP and its structur

25 We previously reported that human endometase/matrilysin-2/matrix metalloproteinase (MMP) 26-mediated

26 Human endometase/matrilysin-2/matrix metalloproteinase-26 (MMP-26) is a n

27 ve biochemical mechanism by which endometase/matrilysin-2/matrix metalloproteinase-26 (MMP-26) may pr

28 We report that matrilysin, a matrix metalloproteinase, is constitutivel

29 Matrilysin, a matrix metalloproteinase, is expressed and

30 ions demonstrate that sulfated GAGs regulate matrilysin activation and its activity against specific

31 forms of chondroitin sulfate did not augment matrilysin activation or activity.

32 with matrix metalloproteinases and regulates matrilysin activity during airway epithelial repair.

33 ts airway re-epithelialization by inhibiting matrilysin activity, contributing to a stereotypic injur

34 itin-2,6-sulfate (CS-D) also did not enhance matrilysin activity, suggesting that the presentation of

35 identify E-cadherin as a novel substrate for matrilysin and indicate that shedding of E-cadherin ecto

36 Both matrilysin and macrophage metalloelastase were detected

37 We report that matrilysin and macrophage metalloelastase, two broad-act

38 ificant increase in the expression levels of matrilysin and macrophage metalloelastase.

39 l lines differed from the HaCaT line in that matrilysin and TIMP-1 proteins were detected in conditio

40 Similarly, MMP-7 (matrilysin) and MT1-MMP (membrane type 1 matrix metallop

41 rated by the action of the metalloproteinase matrilysin, and suggest that matrilysin cleavage of FasL

42 Matrilysin autolytically removes its N-terminal tripepti

43 Active matrilysin bound heparin (K(D), 150 nm) but less so to C

44 single tyrosine residue in the S1' pocket of matrilysin by leucine alters its P1' specificity to rese

45 ific product of myeloperoxidase, inactivates matrilysin by modifying adjacent tryptophan and glycine

46 We have shown that inactivation of MMP-7 (matrilysin) by HOCl coincides with the formation of a no

47 we report that the matrix metalloproteinase matrilysin can process recombinant and cell-associated F

48 three His ligands and the scaffolding of the matrilysin catalytic zinc site are different from that o

49 talloproteinase matrilysin, and suggest that matrilysin cleavage of FasL is an important mediator of

50 In vitro, matrilysin cleaved syndecan-1 from the surface of cells.

51 Furthermore, TIMP-1 and matrilysin co-localized in airway epithelial cells adjac

52 We found that TIMP-1 and matrilysin co-localized in the epithelium of human lungs

53 In vivo, matrilysin co-localized with E-cadherin at the basolater

54 In the involuting mouse prostate, FasL and matrilysin colocalized to the cell surface in a restrict

55 In mouse small intestine, matrilysin colocalized with alpha-defensins (cryptdins)

56 ined to sites of macrophage-elastin contact, matrilysin confers macrophages with their most potent MM

57 Because elastolytically active MME and matrilysin consist only of a catalytic domain (CD), we s

58 These results indicate that while matrilysin contributes to the invasive phenotype, activa

59 Because genetic ablation of matrilysin decreases tumor formation in multiple intesti

60 Matrilysin-deficient (MAT-/-) mice lacked mature cryptdi

61 sure of corneal wounds were observed between matrilysin-deficient and wild-type mice after wounding.

62 vascular endothelial growth factor (VEGF) in matrilysin-deficient and wild-type mouse corneas (experi

63 Matrilysin-deficient human macrophages fail to mediate a

64 Matrilysin-deficient mice (n = 17) and their age-matched

65 re the area of corneal neovascularization in matrilysin-deficient mice and wild-type littermates (exp

66 rences in corneal neovascularization between matrilysin-deficient mice and wild-type littermates seem

67 orneal epithelial cells were not elevated in matrilysin-deficient mice compared with the wild-type mi

68 ation after excimer laser keratectomy in the matrilysin-deficient mice measured 21.3% +/- 5.2% and 18

69 Corneal neovascularization in the matrilysin-deficient mice was confirmed by india ink pre

70 The area of corneal neovascularization in matrilysin-deficient mice was not significantly differen

71 nases, macrophage metalloelastase (MME), and matrilysin degrade insoluble elastin.

72 Biochemical studies showed that matrilysin degraded versican much more efficiently than

73 The properties of this form of matrilysin demonstrate that the propeptide is not essent

74 Mice deficient in matrilysin demonstrated a 67% reduction in the apoptotic

75 Mmp7-/-, and Mmp10-/- mice identified 2,091 matrilysin-dependent and 1,628 stromelysin-2-dependent g

76 Human matrilysin devoid of its propeptide is expressed in Esch

77 ve precursors by matrix metalloproteinase-7 (matrilysin, EC, MMP-7(a)).

78 Thus, to assess matrilysin effects on CD103-E-cadherin interactions in l

79 Furthermore, all matrilysin-expressing benign intestinal tumors of the Mi

80 Mammary tissue from multiparous matrilysin-expressing mice showed decreased FasL express

81 basement membrane disruption was detected in matrilysin-expressing mice, which could account for the

82 factors secreted from monocytes could induce matrilysin expression in a human prostatic cell line.

83 We analyzed matrilysin expression in benign intestinal tumors from m

84 , flagellin-null mutants failed to stimulate matrilysin expression in cultured cells or in lungs infe

85 d physiologically relevant signal regulating matrilysin expression in epithelial cells.

86 e assessed if relevant CF pathogens regulate matrilysin expression in human lung epithelial cells.

87 Matrilysin expression in LNCaP cells was also induced by

88 specifically flagellin) potently stimulates matrilysin expression in lung epithelial cells and may m

89 Matrilysin expression increased apoptosis in the involut

90 a CF isolates and laboratory strains induced matrilysin expression to similar levels.

91 Matrilysin expression was increased in airway epithelial

92 Indeed, matrilysin expression was increased in migrating airway

93 tor released by P. aeruginosa that regulated matrilysin expression.

94 ascular proteoglycan was present at sites of matrilysin expression.

95 MPs with simple domain constituents, such as matrilysin, from the larger and more elaborate enzymes.

96 These observations suggest that matrilysin functions in injury-mediated responses of the

97 Thus, matrilysin functions in intestinal mucosal defense by re

98 tumors, leads to coordinate upregulation of matrilysin gene transcription, contributing to gastroint

99 cient to induce expression of the endogenous matrilysin gene.

100 and cell-associated FasL to sFasL, and that matrilysin-generated sFasL was effective at inducing apo

101 d expression of Bcl-2, Bcl-XL, survivin, and matrilysin, genes associated with a poor prognosis in ad

102 ence of kcat/K(m) for Dns-PLALWAR shows that matrilysin has a broad pH optimum (5.0-9.0) and the pKa

103 icity, the amino acids at these positions in matrilysin have been replaced by those found in stromely

104 siella pneumoniae) induced the expression of matrilysin in Calu-3 lung epithelial cells.

105 tochemistry revealed prominent expression of matrilysin in cells confined to the border between acell

106 The constitutive production of matrilysin in conducting airways, its upregulation after

107 otential mechanism for the overexpression of matrilysin in inflamed ducts and glands of the prostate.

108 e of this study was to determine the role of matrilysin in maintaining corneal avascularity during wo

109 To address the role of matrilysin in Min intestinal tumorigenesis, we generated

110 Bacteria did not induce matrilysin in other cell types, and expression of other

111 compared with wild-type animals, implicating matrilysin in this FasL-mediated process.

112 tivity of matrix metalloproteinase-7 (MMP-7, matrilysin) in vitro, suggesting that this oxidant activ

113 of stromelysin (HFS:L214Y/V215A) to resemble matrilysin increases activity (i.e., higher kcat/KM) tow

114 at loss of FasL is at least one mechanism of matrilysin-induced resistance to apoptosis.

115 ls coexpressing Fas and Fas ligand (FasL) to matrilysin induces apoptosis, whereas chronic exposure t

116 The matrilysin-inducing activity of THP-1 conditioned medium

117 This is the first report of matrilysin induction by an inflammatory cytokine in a ce

118 use model peptides that mimic the region of matrilysin involved in this reaction, VVWGTA, VVWATA, an

119 Glu-198 of human matrilysin is a conserved residue in the matrix metallop

120 Matrilysin is a matrix metalloprotease that is overexpre

121 Matrilysin is a matrix metalloproteinase expressed in th

122 Based on these findings, we conclude that matrilysin is a suppressor of the Min phenotype, possibl

123 We showed previously that matrilysin is a target gene of beta-catenin-Tcf, the tra

124 In normal adult human lung, matrilysin is expressed at low levels in the airway epit

125 Because matrilysin is produced in Paneth cells of the murine sma

126 Although matrilysin is required for closure of epithelial wounds

127 ave demonstrated that one MMP family member, matrilysin, is expressed in a high percentage of early-s

128 entactin with the matrix metalloproteinase, matrilysin, liberates peptides that retain E domain-medi

129 epithelial specific matrix metalloproteinase matrilysin (MAT) has been correlated with enhanced tumor

130 Matrilysin (MAT) prefers leucine over residues that have

131 To examine the role of matrilysin (MAT), an epithelial cell-specific matrix met

132 elial transcriptional responses dependent on matrilysin (matrix metalloproteinase 7 [MMP-7]) and stro

133 erexpression of the matrix metalloproteinase matrilysin (matrix metalloproteinase-7) in the mouse mam

134 Matrilysin (matrix metalloproteinase-7) is expressed by

135 Matrilysin (matrix metalloproteinase-7) is highly expres

136 Matrilysin (matrix metallproteinase-7) is essential for

137 Matrilysin, matrix metalloproteinase (MMP)-7, is upregul

138 Matrilysin may play an important role in maintaining cor

139 TIMP-1 also inhibited matrilysin-mediated cell migration and spreading in vitr

140 Thus, matrilysin-mediated shedding of syndecan-1/KC complexes

141 We demonstrate that matrilysin mediates shedding of E-cadherin ectodomain fr

142 latinase (MMP 9), stromelysin 1 (MMP 3), and matrilysin (MMP 7) all processed this substrate efficien

143 A or B), stromelysins (MMP-3 and MMP-11), or matrilysin (MMP-7) affected SF/HGF-induced responses.

144 No binding to matrilysin (MMP-7) and collagenase 1 (MMP-1) was detecte

145 Unlike the gelatinases (MMP-2 and -9), matrilysin (MMP-7) and collagenases (MMP-1 and -13) are

146 human matrix metalloproteinase (MMP) family, matrilysin (MMP-7) and gelatinase B/type IV collagenase

147 Matrilysin (MMP-7) expression is increased in lung injur

148 ate that HOCl inhibits the activity of human matrilysin (MMP-7) in vitro, suggesting that it might li

149 The matrix metalloproteinase matrilysin (MMP-7) is expressed in the tumor cells of a

150 einases (MMPs) are tightly bound to tissues; matrilysin (MMP-7), although the smallest of the MMPs, i

151 ct of Brij-35 on human gelatinase B (MMP-9), matrilysin (MMP-7), and membrane-type 1 MMP (MT1-MMP) wa

152 pproach for rapid and sensitive detection of matrilysin (MMP-7, a biomarker involved in the degradati

153 lytically active matrix metalloproteinase 7 (matrilysin, MMP-7) and heparin-binding epidermal growth

154 In vivo, matrilysin (MMP7) activates pro-alpha-defensins (procryp

155 Increased matrilysin mRNA levels were detectable at 3 h post-infec

156 In matrilysin null mice, neutrophils remained confined in t

157 tissue, reepithelialization in trachea from matrilysin-null mice was essentially blocked.

158 -injured wild-type mice, but was not shed in matrilysin-null mice.

159 en in wild-type injured tissue was absent in matrilysin-null samples.

160 s C, values closely similar to those for the matrilysin produced by activation of the Chinese hamster

161 ia (Min) mice, we propose that regulation of matrilysin production by beta-catenin accumulation is a

162 -50-fold) and sustained (>24 h) induction of matrilysin production.

163 f factor, LEF-1, significantly downregulated matrilysin promoter activity, suggesting that beta-caten

164 iously observed coordinate regulation of the matrilysin promoter by beta-catenin and Ets family trans

165 gesting that beta-catenin transactivates the matrilysin promoter by virtue of its ability to abrogate

166 IL-1beta activates transcription through the matrilysin promoter in LNCaP cells.

167 The matrilysin promoter is upregulated as much as 12-fold by

168 transfection analyses with a chimeric human matrilysin promoter-chloramphenicol acetyltransferase re

169 nt to effect expression of luciferase from a matrilysin promoter-luciferase reporter plasmid.

170 We propose that matrilysin promotes mammary tumor formation by enhancing

171 We now demonstrate that matrilysin promotes resistance to apoptosis in vivo.

172 lysaccharide treatment substantially induced matrilysin protein and mRNA expression in LNCaP prostate

173 MMP-7 (matrilysin) rapidly cleaved TFPI to a major 35-kDa produ

174 In conclusion, matrilysin regulates pulmonary localization of DC that e

175 lloelastase, stromelysin, collagenase-3, and matrilysin, respectively.

176 The absence of matrilysin resulted in a reduction in mean tumor multipl

177 epithelial cells, transfection of activated matrilysin resulted in shedding of E-cadherin and accele

178 duces apoptosis, whereas chronic exposure to matrilysin selects for apoptosis-resistant cells.

179 helial cells have been repeatedly exposed to matrilysin, show a significant decrease in apoptosis.

180 Our findings suggest that matrilysin, specifically expressed in atherosclerotic le

181 lation was found between mRNA expression for matrilysin, stromelysins 1-3, TIMP-1, or TIMP-3 and secr

182 ne products, a secreted phospholipase A2 and matrilysin, that has been implicated as modifiers of ade

183 omatrilysin and the activity of fully active matrilysin to cleave specific physiologic substrates.

184 In murine intestinal tumors, matrilysin transcripts show striking overlap with the ac

185 Matrilysin was expressed by morphologically normal epith

186 Induction of matrilysin was mediated by a soluble, non-LPS bacterial

187 Matrilysin was not detected in germ-free mice, but the e

188 Matrilysin was present in lipid-laden macrophages, ident

189 ivation or while migrating over wounds, some matrilysin was released basally.

190 s and cell culture studies demonstrated that matrilysin was secreted lumenally by lung epithelium, bu

191 teady-state reaction of wild type and mutant matrilysin with substrates with Leu and Tyr residues in