ライフサイエンスコーパス: meiotic spindle

1 esulting in sperm DNA within 2 microm of the meiotic spindle.

2 required for this loss of cyclin B from the meiotic spindle.

3 own as the spindle pole body or SPB) and the meiotic spindle.

4 GTP is polarized in the cortex overlying the meiotic spindle.

5 anes which envelop and invade the developing meiotic spindle.

6 ics drive the self-organization of a bipolar meiotic spindle.

7 X chromosomes to opposite poles on the first meiotic spindle.

8 romosome to opposite poles on the developing meiotic spindle.

9 nd significantly larger than that of the pig meiotic spindle.

10 s of PKC, results in rapid disruption of the meiotic spindle.

11 t facilitates their proper attachment to the meiotic spindle.

12 here it serves as the precursor to the first meiotic spindle.

13 disrupting organization of the MTOC-TMA and meiotic spindle.

14 TOC-TMA and subsequent assembly of the first meiotic spindle.

15 trosome precludes Golgi association with the meiotic spindle.

16 calreticulin and is notably absent from the meiotic spindle.

17 itioned with respect to their homolog on the meiotic spindle.

18 ocalized to the base of the MTOC-TMA and the meiotic spindle.

19 s that make up knobs to move poleward on the meiotic spindle.

20 oocytes degenerate and fail to form a second meiotic spindle.

21 red for proper structure and function of the meiotic spindle.

22 ulin (colocalized with CaM kinase II) on the meiotic spindle.

23 ents the sperm DNA from interacting with the meiotic spindle.

24 ctly overlying the metaphase-arrested second meiotic spindle.

25 ve reduction in the mobility of knobs on the meiotic spindle.

26 a ring-shaped band of cortex adjacent to the meiotic spindle.

27 the origin of asymmetric positioning of the meiotic spindle.

28 ted irregularities of the zona pellucida and meiotic spindle.

29 12 pN to move a chromosome on the mitotic or meiotic spindle.

30 attachment of unpartnered chromosomes to the meiotic spindle.

31 ogues so they orientate appropriately on the meiotic spindle.

32 a sperm aster that could capture the oocyte meiotic spindle.

33 lizing the bipolar shape of the acentrosomal meiotic spindle.

34 ical F-actin to prevent interaction with the meiotic spindle.

35 ension necessary to align chromosomes on the meiotic spindle.

36 ion of these proteins with p-PKC zeta at the meiotic spindle.

37 the construction and dynamics of mitotic and meiotic spindles.

38 means for increasing microtubule density in meiotic spindles.

39 ndensing chromosomes and associates with the meiotic spindles.

40 length, an idea that has not been tested in meiotic spindles.

41 the MTOC-TMA and subsequent assembly of the meiotic spindles.

42 h as the dynamic asters found in mitotic and meiotic spindles.

43 lel/antiparallel microtubule organization in meiotic spindles.

44 mediated assembly of extremely long bipolar meiotic spindles.

45 nit, MEI-1, cause specific defects in female meiotic spindles.

46 rotubule organization and dynamics in oocyte meiotic spindles.

47 crotubule flux in Xenopus laevis egg extract meiotic spindles.

48 Increased meiotic spindle abnormalities and aneuploidy in oocytes

49 mosomal misalignment on the metaphase plate, meiotic spindle abnormalities, or mitochondrial dysfunct

50 In the absence of centrosomes, meiotic spindles achieve bipolarity by a combination of

51 requires specific positioning of the second meiotic spindle, achieved by dynein-driven transport, an

52 Commonalities in meiotic spindle and chromosome alignment defects under t

53 e more general APC(Fzy), both locally on the meiotic spindle and globally in the egg cytoplasm, to ta

54 d maintaining bipolarity of the acentrosomal meiotic spindle and in promoting the contacts that the c

55 We find that spindlin associates with the meiotic spindle and is modified by phosphorylation in a

56 that global cortical contraction forces the meiotic spindle and overlying membrane out through the c

57 y-pGV oocyte is similar to that of the mouse meiotic spindle and significantly larger than that of th

58 aM kinase II) is tightly associated with the meiotic spindle and that 5 min after egg activation ther

59 product functions in the organization of the meiotic spindle and the formation of long microtubules.

60 imately 2.5-fold between the cortex over the meiotic spindle and the opposite cortex, suggesting that

61 nsition showed PKC-delta associated with the meiotic spindle and then with the chromosomes at MII.

62 lent chromosomes could align normally on the meiotic spindle and whether metaphase spermatocytes woul

63 d oocytes supported the formation of de novo meiotic spindles and, after fertilization with sperm, me

64 toskeleton and required for migration of the meiotic spindle, and a second affecting the spindle micr

65 Xiwi is associated with microtubules and the meiotic spindle, and is localized to the germ plasm--a c

66 of the cortex and proper orientation of the meiotic spindle, and thus we hypothesized that cortical

67 tive mitotic translation occurs on X. laevis meiotic spindles, and a subset of microtubule-bound mRNA

68 Mitotic and meiotic spindles are assemblies of microtubules (MTs) th

69 In animals, female meiotic spindles are attached to the egg cortex in a per

70 many animals, including vertebrates, oocyte meiotic spindles are bipolar but assemble in the absence

71 Meiotic spindles are disorganized, pronuclear migration

72 During female meiosis, meiotic spindles are positioned at the oocyte cortex to

73 etochore microtubules (non-kMTs), vertebrate meiotic spindles are predominantly comprised of non-kMTs

74 We propose that without non-kMTs, metaphase meiotic spindles are similar to mammalian mitotic spindl

75 Anastral meiotic spindles are thought to be organized differently

76 Previous studies have proposed that these meiotic spindles arise from RanGTP-mediated MT nucleatio

77 PKCzeta is tightly associated with the meiotic spindle as determined by detergent extraction an

78 ts indicate that bipolar but abnormal oocyte meiotic spindles assemble in aspm-1(-) embryos, whereas

79 In most animals, female meiotic spindles assemble in the absence of centrosomes;

80 e used to deplete non-kMTs in the vertebrate meiotic spindle assembled in Xenopus egg extracts.

81 lation quantitatively reproduces features of meiotic spindles assembled in Xenopus egg extracts.

82 In contrast to somatic cells, the first meiotic spindle assembles in the absence of centriole-co

83 ill address recent work on the mechanisms of meiotic spindle assembly and chromosome alignment/segreg

84 Accurate meiotic spindle assembly and chromosome segregation - es

85 e they mediate nuclear anchoring, as well as meiotic spindle assembly and rotation, two processes req

86 nes previously shown to contribute to oocyte meiotic spindle assembly are the calponin homology domai

87 s involved in both the timing of location of meiotic spindle assembly as well as the coordination of

88 ependent microtubule reorganization promotes meiotic spindle assembly by facilitating the search and

89 Meiotic spindle assembly commences when microtubules gai

90 ly examine microtubule reorganization during meiotic spindle assembly in living Drosophila oocytes.

91 otor protein in Drosophila that functions in meiotic spindle assembly in oocytes and spindle pole mai

92 on along the pole-to-pole axis, we simulated meiotic spindle assembly in two dimensions using dynamic

93 at overcome arrest exhibit severe defects in meiotic spindle assembly, chromosome segregation, and cy

94 ssed the chromatin-based mechanism of female meiotic spindle assembly, it is less clear how signaling

95 Based on a 2D model of meiotic spindle assembly, we predicted that higher local

96 icrotubule number during the early stages of meiotic spindle assembly.

97 that this regulation is required for bipolar meiotic spindle assembly.

98 itis elegans, is required for bipolar oocyte meiotic spindle assembly.

99 re during the acentrosomal process of oocyte meiotic spindle assembly.

100 ere found to be strongly associated with the meiotic spindle at all stages of meiosis II; however, no

101 We investigated the mechanism by which meiotic spindles become bipolar and the correlation betw

102 tes, fertilized by wild-type sperm, set up a meiotic spindle but do not progress to anaphase I.

103 on of aPKCzeta also causes elongation of the meiotic spindle but still permits spindle migration and

104 , some mechanism must prevent capture of the meiotic spindle by the sperm aster.

105 We used an assay system in which hundreds of meiotic spindles can be observed forming around chromati

106 or the proper functioning of the mitotic and meiotic spindle checkpoints (MSCs), which monitor the in

107 est; extrusion rather than extraction of the meiotic spindle-chromosome complex (SCC); nuclear transf

108 , and is concentrated at the point where the meiotic spindle contacts the F-actin-rich cortex.

109 Meiotic spindles contain more microtubules than their mi

110 Our data suggest that non-kMTs in meiotic spindles contribute to normal kMT dynamics, stab

111 Mispositioning of the meiotic spindle, defects in polar body extrusion and chr

112 ry of female meiosis and the polarity of the meiotic spindle dictate that the partner with the greate

113 In contrast, we find that the female meiotic spindle does not scale as closely to egg size, a

114 a wave that initiates in the vicinity of the meiotic spindle during anaphase I.

115 ng meiotic maturation and concentrate on the meiotic spindle during metaphases I and II.

116 d on the vegetal side (the side opposite the meiotic spindle) during maturation.

117 scopy identified that Kif2a localized to the meiotic spindle, especially concentrated at the spindle

118 P identified that Cep55 was localized to the meiotic spindle, especially to the spindle poles at meta

119 In the absence of DAZL synthesis, the meiotic spindle fails to form due to disorganization of

120 These studies identify meiotic spindle formation and programmed degradation of

121 nucleolus chromatin morphology, and abnormal meiotic spindle formation was observed following oocyte

122 peroxisome biogenesis, endosome sorting and meiotic spindle formation, but functions for the SF7 AAA

123 Meiotic spindle formation, however, is not affected in t

124 icrotubule-severing activity and its role in meiotic spindle formation, we analyzed the MEI-1(A338S)

125 t loss of telomere-LINC contacts compromises meiotic spindle formation.

126 ompensate for the lack of centrosomes during meiotic spindle formation.

127 ed mechanisms underlying differences between meiotic spindles formed in egg extracts of two frog spec

128 gamma-Tubulin was stably associated with the meiotic spindle from prometaphase-1 through to anaphase-

129 f the spindle to BAPTA and EGTA-suggest that meiotic spindle function in frog oocytes requires highly

130 esin-like protein, SUB, that is required for meiotic spindle function.

131 s I and meiosis II, and dissociates from the meiotic spindle in anaphase II.

132 shwork that facilitates translocation of the meiotic spindle in asymmetric division of mouse oocytes.

133 the proper organization of the acentrosomal meiotic spindle in Drosophila melanogaster oocytes.

134 ts in plants, the asymmetric position of the meiotic spindle in mammalian embryos, and the developmen

135 We have studied the formation of the first meiotic spindle in murine oocytes from mice homozygous f

136 nstrated the variable position of the second meiotic spindle in relation to the first polar body; con

137 Female meiotic spindles in many organisms form in the absence o

138 Most meiotic spindles in mei-38 oocytes are bipolar but poorl

139 Human oocytes assembled a meiotic spindle independently of either centrosomes or o

140 In contrast, Caenorhabditis elegans female meiotic spindles initially shorten in the pole-to-pole a

141 lead to circular mitotic figures and loss of meiotic spindle integrity.

142 that CSNK-1 prevents expulsion of the entire meiotic spindle into a polar body by negatively regulati

143 During female meiosis in animals, the meiotic spindle is attached to the egg cortex by one pol

144 In mouse oocytes, the first meiotic spindle is formed through the action of multiple

145 aberrant chromosome behavior a stable first meiotic spindle is not formed, the spindle poles continu

146 In animals, the female meiotic spindle is positioned at the egg cortex in a per

147 The meiotic spindle is thought to provide a scaffold that me

148 for the assembly and maintenance of bipolar meiotic spindles, is not needed to maintain spindle bipo

149 ought to play a major role in organizing the meiotic spindle, it remains unclear how a stable bipolar

150 ave also been reported for C. elegans female meiotic spindles, it is not clear whether they are repre

151 The female meiotic spindle lacks a centrosome or microtubule-organi

152 embly of this bipolar spindle, and while the meiotic spindle lacks traditional centrosomes, some cent

153 hip between microtubule disassembly rate and meiotic spindle length.

154 We have characterized male meiotic spindle lengths in wild-type and the ask1-1 muta

155 Although mitotic and meiotic spindles maintain a steady-state length during m

156 the hermaphrodite gonad and is localized to meiotic spindle microtubules in the newly fertilized emb

157 in formation and maintenance of mitotic and meiotic spindles, move in opposite directions along micr

158 cent protein (GFP) to monitor changes in the meiotic spindle of live oocytes after activation in vitr

159 The meiotic spindles of animal eggs move to extremely asymme

160 ulates the production of microtubules in the meiotic spindles of Caenorhabditis elegans oocytes.

161 sted oocytes are relatively stable, however, meiotic spindles of in vitro-activated oocytes are highl

162 Meiotic spindles of metaphase-arrested oocytes are relat

163 alpha 85E does not disrupt meiotic spindle or cytoplasmic microtubules but causes d

164 gation-promoting chromosome alignment on the meiotic spindle or some physical interaction between hom

165 ds to a significant (P < 0.05) disruption of meiotic spindle organization and chromosome alignment du

166 MTOC-associated proteins and plays a role in meiotic spindle organization in mammalian oocytes.

167 nts show that HSET activity is essential for meiotic spindle organization in murine oocytes and taxol

168 role of protein kinase C delta (PKCdelta) on meiotic spindle organization was evaluated in mouse oocy

169 es, such as chromosome coalescence, aberrant meiotic spindle organization, and the expression of a me

170 motor proteins are essential for mitotic and meiotic spindle organization, chromosome segregation, or

171 Oocyte meiotic spindles orient with one pole juxtaposed to the

172 microtubule severing and ASPM-1 both promote meiotic spindle pole assembly in C. elegans oocytes, whe

173 t2p is required for linking telomeres to the meiotic spindle pole body (SPB) but not for attachment o

174 ion factor EAP30, Dot2, negatively regulates meiotic spindle pole body (SPB, the yeast equivalent of

175 n, PKCdelta expression was restricted to the meiotic spindle poles and a few specific cytoplasmic foc

176 TAC-1 is initially enriched at the meiotic spindle poles and is later recruited to the sper

177 n with microtubules and with LIN-5-ASPM-1 at meiotic spindle poles and that the APC promotes spindle

178 The acentrosomal meiotic spindle poles do not have centrioles and are not

179 g enzyme that is concentrated at mitotic and meiotic spindle poles in animals.

180 hat phosphorylated GM130 associates not with meiotic spindle poles, but with ER clusters in the matur

181 P-190, and CP-60 are not concentrated at the meiotic spindle poles.

182 ity, is required for assembly of acentriolar meiotic spindle poles.

183 Although meiotic spindle positioning in oocytes has been investig

184 try in oocytes by participating in eccentric meiotic spindle positioning, sperm incorporation cone dy

185 tcc-1 and unc-116 causes similar defects in meiotic spindle positioning, supporting the concept of T

186 terestingly, in embryos lacking an organized meiotic spindle, produced either by nocodazole treatment

187 is defined by the position of the persistent meiotic spindle rather than by the position of the sperm

188 ules, we have characterized the movements of meiotic spindles relative to the cell cortex.

189 Assembly of Caenorhabditis elegans female meiotic spindles requires both MEI-1 and MEI-2 subunits

190 Wild-type C. elegans meiotic spindle shortening proceeds through an early kat

191 In frogs, the meiotic spindle size is positively correlated with the e

192 Importantly, the disruption of meiotic spindle stability was associated with decreased

193 MTOCs, which functions in the regulation of meiotic spindle stability.

194 Defects in meiotic spindle structure can lead to chromosome segrega

195 Defects in meiotic spindle structure contribute to chromosome segre

196 sted oocytes were characterized by disrupted meiotic spindle structure, reduced microtubule density a

197 es have been found to be enriched at the MII meiotic spindle, such as Protein Kinase C (PKC), and are

198 cal granule-free domain (CGFD) overlying the meiotic spindle that is in close proximity to the cortex

199 ays, whereas weaker mutants assemble bipolar meiotic spindles that are longer than wild type.

200 d from the mutants revealed microtubules and meiotic spindles that were close to normal even in the a

201 and orient the homologous chromosomes on the meiotic spindle to ensure proper segregation at meiosis

202 tive mutants causes both halves of the first meiotic spindle to extend symmetrically toward opposing

203 microtubule severing in translocation of the meiotic spindle to the cortex.

204 asymmetry results from the anchoring of the meiotic spindle to the oocyte cortex and subsequent cort

205 n range from the assembly and positioning of meiotic spindles to the prevention of cytoplasmic stream

206 t be consistent with direct transport of the meiotic spindle toward the cortex by kinesin-1.

207 identified a motor complex that may move the meiotic spindle toward the cortex in Caenorhabditis eleg

208 t step in asymmetric positioning of anastral meiotic spindles, translocation to the cortex.

209 atisfaction was achieved because the bipolar meiotic spindle was assembled more quickly in the absenc

210 osin-10 (Myo10) is important for assembly of meiotic spindles, we assessed the role of this unconvent

211 When two meiotic spindles were brought close enough together, the

212 ays a subtle role in formation of the female meiotic spindle which is acentriolar, but is essential f

213 y dependent on asymmetric positioning of the meiotic spindle, which is established through migration

214 n in eukaryotes depends upon the mitotic and meiotic spindles, which assemble at the time of cell div

215 n at the opposite end of the embryo from the meiotic spindle while yolk granules are transported thro

216 exposure and suggest that the oocyte and its meiotic spindle will provide a sensitive assay system fo

217 onsense mutation in MEI-1 caused assembly of meiotic spindles without any poles as assayed by localiz