ライフサイエンスコーパス: membrane microdomain

1 an increase in the unsaturation index in the membrane microdomain.

2 glutinin (HA), which is also found in plasma membrane microdomains.

3 rgeted to synapses and clustered in modified membrane microdomains.

4 lux by clustering NMDA receptors in modified membrane microdomains.

5 anize--they are not templated by preexisting membrane microdomains.

6 dt) is dependent upon the integrity of lipid membrane microdomains.

7 fts by redistributing cholesterol into these membrane microdomains.

8 singly to identify clustering of proteins in membrane microdomains.

9 in localizes a portion of Galpha(s) to these membrane microdomains.

10 hieved by receptor exclusion from lipid raft membrane microdomains.

11 ents are associated with detergent-resistant membrane microdomains.

12 hat N-myristoylated cystin fractionates with membrane microdomains.

13 at M. truncatula FLOT2 and FLOT4 localize to membrane microdomains.

14 via Ca(2+) activation of AC activity within membrane microdomains.

15 PKC-alpha within intact cholesterol-enriched membrane microdomains.

16 signaling events in these specialized plasma membrane microdomains.

17 y regulating trafficking to cholesterol-rich membrane microdomains.

18 ith phospholipase D2 (PLD2) in caveolin-rich membrane microdomains.

19 roteins preferentially colocalize with fluid membrane microdomains.

20 f proteins, including some connexins, within membrane microdomains.

21 ne potential site of action is in lipid raft membrane microdomains.

22 of the protein to reside in cholesterol-rich membrane microdomains.

23 perturb membrane structure by alteration of membrane microdomains.

24 marker protein that is enriched in caveolae membrane microdomains.

25 ngolipid stereochemistry in the formation of membrane microdomains.

26 plasma membranes, consistent with localized membrane microdomains.

27 ively transfer GSL to or from these putative membrane microdomains.

28 ral additional gamma-secretase substrates in membrane microdomains.

29 45, suggesting that they arise from distinct membrane microdomains.

30 erized as caveolin and flotillin-rich plasma membrane microdomains.

31 nhances DAT localization in cholesterol rich membrane microdomains.

32 and phospholipase D2 (PLD2) in caveolin-rich membrane microdomains.

33 cells cluster signaling complexes in plasma membrane microdomains.

34 The levels of GM3 in the cell also affect membrane microdomains.

35 vivo with sphingolipid- and cholesterol-rich membrane microdomains.

36 hospholipase D2 colocalized in caveolin-rich membrane microdomains.

37 play a role in organizing the G protein into membrane microdomains.

38 iled to translocate into glycolipid-enriched membrane microdomains.

39 gered by changes in the lipid composition of membrane microdomains.

40 d VRCs for robust replication in PE-enriched membrane microdomains.

41 dt) is dependent upon the integrity of lipid membrane microdomains.

42 nhances DAT localization in cholesterol-rich membrane microdomains.

43 ng molecules at subcellular compartments and membrane microdomains.

44 odifying insulin receptor translocation into membrane microdomains.

45 orters that, nonetheless, remain confined to membrane microdomains.

46 rkers were recently developed to investigate membrane microdomains.

47 ynamic changes in its localization in plasma membrane microdomains.

48 d that prestin localizes in cholesterol-rich membrane microdomains.

49 e clustering of MHC-II present in lipid raft membrane microdomains, a process that leads to MHC-II en

50 ly reside in specialized detergent-resistant membrane microdomains, act as signaling scaffolds.

51 Disrupting the structure of membrane microdomains after gp120 treatments restored th

52 rofile is reminiscent of detergent-insoluble membrane microdomains, although our approach is valuably

53 te the distribution of prestin within plasma membrane microdomains and affect prestin self-associatio

54 teins are spatially regulated between plasma membrane microdomains and between the plasma membrane an

55 -FRET, we revealed that AtHIR1 is present in membrane microdomains and co-localizes with the membrane

56 depletion microscopy the key roles played by membrane microdomains and cytoskeleton transient organiz

57 ds, leading to a reduced inclusion in plasma membrane microdomains and decreased uptake by caveolar e

58 f PP2A and PP2A regulatory enzymes in plasma membrane microdomains and identify a novel methylation-d

59 ls by pDCs involves CD81- and CD9-associated membrane microdomains and induces potent IFN-alpha produ

60 s an adaptor module that targets rPGRP-LC to membrane microdomains and interacts with the negative re

61 ncorporated into and localized within plasma membrane microdomains and proximal vesicles in T cells.

62 the basally trafficked pool associated with membrane microdomains and SNAP25.

63 gle-particle tracking analysis revealed that membrane microdomains and the cytoskeleton, especially m

64 rological synapses, providing a link between membrane microdomains and the formation of polarized mem

65 Sphingolipids represent a major component of membrane microdomains, and ceramide-enriched microdomain

66 esterol and sphingolipid-enriched lipid raft membrane microdomains, and delivery of protein ligands t

67 cally associated with RPS2, are localized in membrane microdomains, and quantitatively contribute to

68 es are differentially regulated in different membrane microdomains, and the overall activity of this

69 there is increasing evidence to suggest that membrane microdomains, and their modulation, have an imp

70 e organization of the GnRHR into low density membrane microdomains appears critical in mediating GnRH

71 FCS measurements in small-membrane microdomains (approximately 0.2 microm2) reveal

72 Membrane microdomains are assembled by lipid partitionin

73 Because membrane microdomains are involved in inducing growth an

74 ence that cholesterol- and sphingolipid-rich membrane microdomains are involved in regulating traffic

75 ther, these data demonstrate that structured membrane microdomains are necessary for ceramide-induced

76 used on lipid rafts and confirmed that these membrane microdomains are required for IL-6 and IGF-I si

77 elated proteins implicated in scaffolding of membrane microdomains, are rapidly recruited to the urop

78 To determine how such membrane microdomains arise during chondrocyte maturatio

79 recent work has highlighted sphingolipids in membrane microdomains as potential targets for inhibitio

80 y described Incs were localized to inclusion membrane microdomains, as evidenced by colocalization wi

81 DCA stimulated TGR5 redistribution to plasma membrane microdomains, as localized by immunogold electr

82 , CD4 and G protein were present in separate membrane microdomains, as shown by double-label immunoel

83 ObRa because of differential involvement of membrane microdomains, as shown by use of the clathrin i

84 r to involve a general reorganization of the membrane microdomains associated with virion assembly, b

85 Our previous work has shown that the membrane microdomain-associated flotillin proteins are p

86 herin is localized to a spatially restricted membrane microdomain at the apical inner segment recess

87 s well, by recruiting TCR/pMHCI complexes to membrane microdomains at a rate which depends on the aff

88 6 mol% in the cytoplasmic leaflet of plasma membrane microdomains at sites of docked vesicles.

89 sses, such as protein sorting, organelle and membrane microdomain biogenesis, protein-protein interac

90 precursors show evidence of Par3-expressing membrane microdomains, but fail to develop normal apical

91 llular matrix proteins in specialized plasma membrane microdomains, but the effects of these interact

92 Disruption of cholesterol-rich membrane microdomains by acute exposure of cells to meth

93 the extent of organization of proteins into membrane microdomains by analyzing the distribution of p

94 Recently, near-membrane microdomain Ca(2+) transients were identified i

95 -1 (Cav-1), the major protein of specialized membrane microdomains called caveolae, which functions i

96 Exosome uptake depends on cholesterol-rich membrane microdomains called lipid rafts, and can be blo

97 teins, including many signaling proteins, to membrane microdomains, called lipid rafts.

98 ocalization with these receptors on the same membrane microdomain can also recruit thrombin to activa

99 and cholesterol/sphingolipid-enriched plasma membrane microdomain caveolae were also observed.

100 estigate the role of cholesterol-rich plasma membrane microdomains (caveolae and lipid rafts) in GH s

101 The relationships between membrane microdomains, cholesterol biosynthesis, and end

102 of PrP(C) in specific, cholesterol-sensitive membrane microdomains, commonly called lipid rafts.

103 raction of SHP-1 constitutively localizes to membrane microdomains, commonly referred to as lipid raf

104 vation and demonstrate the essential role of membrane microdomain compartmentalization in enabling PI

105 gether, these results suggest that different membrane microdomain components are recruited in a stepw

106 he first time that dietary DHA alters T cell membrane microdomain composition and suppresses the PKCt

107 Unfortunately, little is known about how membrane microdomain composition controls factor VIIa-ti

108 oth clathrin-deficient and clathrin-enriched membrane microdomains concurrent with diminished tyrosin

109 We now show that APC lipid raft membrane microdomains contain specific class II-peptide

110 APP induced the formation of cellular plasma membrane microdomains containing dense lipids, in additi

111 These results suggest that separate membrane microdomains containing either viral or host pr

112 lly, cholesterol extraction destabilizes the membrane microdomains containing Lck, while the rafts co

113 ltured cells or purified detergent-resistant membrane microdomains containing ShhNp.

114 rkably, both serum EV-enriched fractions and membrane microdomains containing the acquired MHC alloan

115 The ExPortal of Streptococcus pyogenes is a membrane microdomain dedicated to the secretion and fold

116 h active clustering of relevant molecules in membrane microdomains defined as the supramolecular acti

117 We studied the function of plasma membrane microdomains defined by the proteins flotillin

118 e (AMPH) induced DAT-meditated DA efflux and membrane microdomain distribution of the transporter.

119 ne-induced DAT-mediated dopamine efflux, and membrane microdomain distribution of the transporter.

120 Detergent-resistant membrane microdomains (DRM) rich in cholesterol and sphi

121 roteomic quantitation of detergent resistant membrane microdomains (DRMMs) isolated from cells expres

122 ted with Ib, after which detergent-resistant membrane microdomains (DRMs) were extracted with cold Tr

123 eins that associate with detergent-resistant membrane microdomains (DRMs), which are known to be invo

124 ficial membrane bilayers containing discrete membrane microdomains encompassing T cell ligands (i.e.,

125 ogenes is an anionic phospholipid-containing membrane microdomain enriched in Sec translocons and pos

126 t a reporter protein to Pd, likely to plasma membrane microdomains enriched at Pd As such, the GPI mo

127 ient, suggesting that TBSV replicates within membrane microdomains enriched for PE.

128 Lipid rafts are conceptualized as membrane microdomains enriched in cholesterol and glycos

129 Lipid rafts are membrane microdomains enriched in cholesterol and sphing

130 Lipid rafts are membrane microdomains enriched in cholesterol and sphing

131 f photoreceptor CNG channel association with membrane microdomains enriched in raft lipids, cholester

132 e viral RdRP and host factors to subcellular membrane microdomains enriched with specific phospholipi

133 bined with their residence in lipid-enriched membrane microdomains facilitates rapid, high-capacity s

134 IV-1) relies on cholesterol-laden lipid raft membrane microdomains for entry into and egress out of s

135 lesterol-dependent CEACAM localizations into membrane microdomains for MHV entry, instead suggesting

136 namically form hetero-oligomers and organize membrane microdomains for protein complexes.

137 not depend on detergent-insoluble, raft-like membrane microdomains for stability.

138 (1) Statins modulate membrane microdomain formation, resulting in reduced exp

139 bisphosphate (PtdIns(4,5)P2) and in inducing membrane microdomain formation.

140 ADAM10 is compartmentalized into membrane microdomains formed by tetraspanins, which are

141 These membrane microdomains from diverse bacteria harbor homol

142 y dampened by inhibition of host cell plasma membrane microdomain function.

143 argeting of ion channels to cholesterol-rich membrane microdomains has emerged as a novel mechanism o

144 Lipid rafts, sterol- and sphingolipid-rich membrane microdomains, have been extensively studied in

145 known to be compartmentalized within plasma membrane microdomains; however, the underlying mechanism

146 Lipid rafts and caveolae are specialized membrane microdomains implicated in regulating G protein

147 agic pathway and the recruitment to specific membrane microdomains in a physiological human gene expr

148 activity of LMP1 when recruited to the same membrane microdomains in B cells.

149 We examined the surface distribution of raft membrane microdomains in cortical neuron cultures during

150 evidence indicates the growing importance of membrane microdomains in health and disease.

151 To examine dynamic Akt activity in membrane microdomains in living cells, we developed a sp

152 ion is to colocalize signaling proteins with membrane microdomains in order to facilitate their inter

153 idence for a critical role of Flot1-enriched membrane microdomains in PKC-triggered DAT endocytosis a

154 Our results implicate REM1.3 membrane microdomains in plant susceptibility to an oomy

155 Although the importance of membrane microdomains in receptor-mediated activation of

156 also required to localize DAT within plasma membrane microdomains in stable cell lines, and was esse

157 We investigated the role of host cell membrane microdomains in the entry of F. tularensis subs

158 The role of plasma-membrane microdomains in the organization of signaling p

159 Lipid rafts, specialized membrane microdomains in the plasma membrane rich in cho

160 e Env incorporation and the role of specific membrane microdomains in this process.

161 itutively and exclusively localized to these membrane microdomains in various experimental models.

162 ese findings demonstrate a critical role for membrane microdomains in vesicular trafficking-mediated

163 l a novel modality by which n-3 PUFA remodel membrane microdomains in vivo and thereby alter caveolae

164 x protein (M protein) partitions into plasma membrane microdomains in VSV-infected cells as well as i

165 Activated IL-7 receptors embedded in membrane microdomains induce actin-microfilament meshwor

166 pose in Cell that expansion of intracellular membrane microdomains induced by saturated FA recruit an

167 which the formation of specific sphingolipid membrane microdomains initiates signaling cascades that

168 ch as secretion, endocytosis, segregation in membrane microdomains, intracellular transport, and targ

169 The OX40 signalosome is formed in membrane microdomains irrespective of TCR engagement, an

170 The formation of dynamic membrane microdomains is an important phenomenon in many

171 Detection of cholesterol-rich membrane microdomains is confirmed by observation of the

172 d DR4 and DR5, respectively) into lipid raft membrane microdomains is required for TRAIL-induced cell

173 Specialized membrane microdomains known as lipid rafts are thought t

174 Significance statement: Membrane microdomains known as lipid rafts have been pro

175 with cholesterol- and sphingolipid-enriched membrane microdomains (lipid rafts) and are highly expre

176 that cholesterol- and sphingolipid-enriched membrane microdomains (lipid rafts) mediate specific gui

177 k) signaling module from the GM1-rich plasma membrane microdomains (lipid rafts), and subsequently an

178 that it may partition into cholesterol-rich membrane microdomains (lipid rafts), its compartmentaliz

179 (Lck) from sphingolipid-cholesterol-enriched membrane microdomains (lipid rafts).

180 s or by altering cellular structures such as membrane microdomains (lipid rafts).

181 f sterol normally occurred within membranes, membrane microdomains (lipid rafts/caveolae), and intrac

182 ng of MOG into glycosphingolipid-cholesterol membrane microdomains ("lipid rafts"), followed by chang

183 tein Gag associates with two types of plasma membrane microdomains, lipid rafts and tetraspanin-enric

184 that CD11b/CD18 bound APC within specialized membrane microdomains/lipid rafts and facilitated APC cl

185 ed sGC activity related to dynamic shifts in membrane microdomain localization, with Cav3-microdomain

186 brane microdomains and co-localizes with the membrane microdomain marker REM1.3.

187 Thus, GM3-dependent membrane microdomains might be essential for the proper

188 hocytes where formation of ceramide-enriched membrane microdomains modulates TCR signaling.

189 We asked what determines the cell membrane microdomain of CAR.

190 lioside-enriched, detergent-resistant plasma membrane microdomains of antigen-presenting cells.

191 upied inactive receptor (R) conformations in membrane microdomains of individual cells.

192 he distribution of single LTCCs in different membrane microdomains of nonfailing and failing human an

193 roscopy showed that both were organized into membrane microdomains of similar sizes, approximately 10

194 GluR1, and ERK all reside within specialized membrane microdomains of the DH, and that ERalpha and ER

195 etero-oligomers in vivo and were enriched in membrane microdomains of the plasma membrane.

196 d further by tissue factor partitioning into membrane microdomains on some cell surfaces.

197 at preclustering of MHC-peptide complexes in membrane microdomains on the APC surface affects the eff

198 of the epithelial cells showed formation of membrane microdomains only during coinfection.

199 locates and concentrates in cholesterol-rich membrane microdomains or lipid rafts, facilitating forma

200 enters corneal epithelial cells in vitro via membrane microdomains or lipid rafts.

201 that is associated with detergent-resistant membrane microdomains or lipid rafts.

202 rols can promote or inhibit the formation of membrane microdomains or lipid rafts.

203 zed, and the involvement of cholesterol-rich membrane microdomains, or lipid rafts, in the life cycle

204 It has been controversial at which membrane microdomains PDGFRs reside and how they control

205 Our results indicate that cholesterol-rich membrane microdomains play a role in transmitting non-ge

206 r luminal acidification nor cholesterol-rich membrane microdomains play essential roles in soluble co

207 Epidermal growth factor receptor (EGFR) at membrane microdomains plays an essential role in the gro

208 embrane compositions, engineering metastable membrane microdomains, probing 2D lipid-lipid mixing, an

209 afts, distinct cholesterol/sphingolipid-rich membrane microdomains, provide a mechanism for this regu

210 physiological functions, and its presence in membrane microdomains (rafts) appears to be important fo

211 In summary, this study showed membrane microdomains (rafts/caveolae) isolated by three

212 Membrane microdomains ("rafts") that sequester specific

213 a are co-enriched in cholesterol-rich plasma membrane microdomains/rafts purified from N2a cells.

214 partitioning of Rac1 to caveolin-containing membrane microdomains, raising the possibility that MIF

215 signaling events compartmentalized by these membrane microdomains, recent studies have revealed the

216 to occur via multiple mechanisms, including membrane microdomains, receptor oligomerization, and pro

217 Plasma-membrane microdomains referred to as lipid rafts have al

218 nents are organized into detergent-resistant membrane microdomains referred to as lipid rafts.

219 ch lateral segregation into specialized raft membrane microdomains regulates the activable pool of nA

220 directed there or how localization to these membrane microdomains regulates Trk signaling.

221 ling, and indicate that septins may organize membrane microdomains relevant to other signalling proce

222 patiotemporal regulation of Akt in different membrane microdomains remains largely unknown.

223 of Chl and PSI complexes are colocated in a membrane microdomain requiring PG for integrity.

224 Lipid raft domains are specialized membrane microdomains rich in cholesterol and sphingolip

225 ectively partitioned into specialized plasma membrane microdomains rich in glycosphingolipids and cho

226 Neither the cytoskeleton nor membrane microdomain structure was involved in constrain

227 cellular model, we found LRRK2 to locate to membrane microdomains such as the neck of caveolae, micr

228 Membrane microdomains, such as caveolae and rafts, are e

229 We find several mechanisms by which membrane microdomains, such as lipid rafts, reduce these

230 Most importantly, membrane microdomain targeting of these proteins was upr

231 rgeted to cholesterol- and sphingolipid-rich membrane microdomains termed "lipid rafts." However, the

232 sitide signaling are mediated through plasma membrane microdomains termed caveolae/lipid rafts.

233 these diverse activities, we examined plasma membrane microdomains termed eisosomes or membrane compa

234 he BCR in sphingolipid- and cholesterol-rich membrane microdomains termed lipid rafts.

235 be cholesterol- and sphingolipid-rich plasma membrane microdomains termed lipid rafts.

236 MHC-II also associates with another type of membrane microdomain, termed tetraspan microdomains.

237 (MHC-II) associate with detergent-resistant membrane microdomains, termed lipid rafts, which affects

238 ubset of BACE1 localizes to cholesterol-rich membrane microdomains, termed lipid rafts.

239 al synapse by recruiting detergent-resistant membrane microdomains, termed lipid rafts.

240 ex and dynamic topographical organization of membrane microdomains than is predicted by biochemical a

241 oligovalent ligation positions the BCR in a membrane microdomain that is distinct from one engaged i

242 Lipid rafts are membrane microdomains that are functionally distinct fro

243 of EPCR is localized on the cell surface in membrane microdomains that are positive for caveolin-1.

244 Lipid rafts are membrane microdomains that are proposed to function as p

245 Lipid rafts are cholesterol-rich membrane microdomains that are thought to act as coordin

246 hymal cells contain discrete Par3-expressing membrane microdomains that become restricted to an apica

247 Caveolae are plasma membrane microdomains that can compartmentalize proteins

248 fic MHC class II-peptide complexes in plasma membrane microdomains that can facilitate efficient T ce

249 nduces the formation of cholesterol-enriched membrane microdomains that compartmentalize its activate

250 gest the formation of phosphatidate-enriched membrane microdomains that contain all components of the

251 However, virus budding occurred from membrane microdomains that contained both G protein and

252 endowed with caveolae, which are specialized membrane microdomains that facilitate the integration of

253 st the existence of lipid rafts, specialized membrane microdomains that promote interaction among sig

254 thelial cells have been implicated as plasma membrane microdomains that sense or transduce hemodynami

255 athways and becomes concentrated in specific membrane microdomains that serve as signaling platforms.

256 s the inherent property of partitioning into membrane microdomains that then serve as the sites of as

257 -sensitive nAChR might reside in specialized membrane microdomains that upon cholesterol depletion be

258 s indicate that NaPi protein is localized in membrane microdomains, that in potassium deficiency a la

259 The integrity of one type of APC membrane microdomain, the lipid raft, is important for a

260 Disruption of cholesterol-rich membrane microdomains, the localization site of CD81, or

261 lation affected the association of GP64 with membrane microdomains, the potential association of GP64

262 sion by condensing CEACAMs into "lipid raft" membrane microdomains, thereby creating opportunities fo

263 gulate the integration and/or coalescence of membrane microdomains, thereby establishing apical-basal

264 ass II complexes are clustered in APC plasma membrane microdomains, thereby providing a mechanism for

265 To study subcellular PDGFR activity at membrane microdomains, this PDGFR biosensor was further

266 specific effectors into function-specifying membrane microdomains to carry out receptor trafficking.

267 ears to modify preexisting sphingolipid-rich membrane microdomains to fuse and form ceramide-enriched

268 We report localization of lipid membrane microdomains to specific "poles" of asymmetric

269 redistributes from nonlipid raft (LR) to LR membrane microdomains upon immunoglobulin G-red blood ce

270 a1- and beta2-adrenergic receptor-associated membrane microdomains using a novel membrane-targeted Fo

271 n when we prevented it from associating with membrane microdomains via the GPI anchor or when we inhi

272 flotillin, an intrinsic constituent of these membrane microdomains, via the adapter protein, c-Cbl as

273 junction with the known heterogeneity of OHC membrane microdomains, voltage-gated ion channels, charg

274 particles that bud from GSL-enriched plasma membrane microdomains was also dependent on interactions

275 rine substitutions (4S) failed to cluster in membrane microdomains, was deficient in restriction of p

276 To visualize the FAK activity at different membrane microdomains, we develop a fluorescence resonan

277 herin and their affiliation with "raft-like" membrane microdomains were modified by these cytokines.

278 nalog, the L-threo analog did not cluster in membrane microdomains when added at higher concentration

279 ramatically increased by targeting it to the membrane microdomains where fusion occurs, via the addit

280 d-rafts and can target functional enzymes to membrane microdomains where pathologic APP-processing is

281 t activated PKCalpha is recruited to FR-rich membrane microdomains where, in association with its rec

282 P and BACE1 were colocalized into stabilized membrane microdomains, where the beta-cleavage of APP an

283 CMA, only occurs outside the lipid-enriched membrane microdomains, whereas the LAMP-2A located withi

284 It is enriched in lipid raft membrane microdomains, which are also the sites of assem

285 assembly platforms might involve sterol-rich membrane microdomains, which are heterogeneous and highl

286 BMP receptor association with membrane microdomains, which is necessary for BMP signal

287 ves in growth, signaling, and maintenance of membrane microdomains, which may arise from the unique c

288 hat facilitate the formation of postsynaptic membrane microdomains, which may serve key roles in the

289 activity is evenly distributed at different membrane microdomains, while integrin-mediated signaling

290 annel localizes in caveolae, which are known membrane microdomains whose major component in the stria

291 t required to concentrate the G protein into membrane microdomains with a density similar to that of

292 d in plasma membranes of infected cells into membrane microdomains with diameters of 100 to 150 nm, w

293 e G protein was organized predominantly into membrane microdomains with diameters of approximately 10

294 Therefore, FAK is activated at membrane microdomains with distinct activation mechanism

295 ible mechanisms: (i) clustering of PIP(2) in membrane microdomains with restricted lateral diffusion,

296 ergents, indicative of their co-residence in membrane microdomains with similar protein-lipid composi

297 in2 [6-8], is sufficient to generate de novo membrane microdomains with some of the predicted propert

298 have been reported to localize to different membrane microdomains, with H-Ras localizing to caveolin

299 esponsiveness of Ca2+ signaling complexes at membrane microdomains, with the most responsive complexe

300 strategy to explore the function of BACE1 in membrane microdomains without altering the cellular chol