ライフサイエンスコーパス: membrane preparation

1 Ca(2+) stored in sperm (and from microsomal membrane preparations).

2 he single tagged species were mixed prior to membrane preparation.

3 a 105-110-kDa polypeptide in the undigested membrane preparation.

4 adenylyl cyclase activity in a neuroblastoma membrane preparation.

5 to that of total cell lipids and to a plasma membrane preparation.

6 These Fabs also reacted with a virion membrane preparation.

7 previously accomplished in a broken cell or membrane preparation.

8 esting for protease sensitivity in an oocyte membrane preparation.

9 t is available in high abundance in a native membrane preparation.

10 other cofactors that are diluted out in the membrane preparation.

11 40 from cannabinoid receptors in a rat brain membrane preparation.

12 a2Rs) in live cells and neurons, but not for membrane preparations.

13 in mouse brain and transfected HEK-293 cell membrane preparations.

14 gonists decrease basal G-protein activity in membrane preparations.

15 rane proteins and the protein composition of membrane preparations.

16 d with cells was 4- to 6-fold higher than in membrane preparations.

17 t analysis of guinea-pig ventricular myocyte membrane preparations.

18 the native receptor in bovine adrenocortical membrane preparations.

19 ranes, a small fraction of each was found in membrane preparations.

20 us requiring all experiments to be done with membrane preparations.

21 tion were in accord with those expected from membrane preparations.

22 l cultures and is present in synaptic plasma membrane preparations.

23 e disorder present in partially oriented 1-D membrane preparations.

24 ts of Gi2 and Gi3, but not Gi1, in all three membrane preparations.

25 ine to measure nAChR in cells in situ and in membrane preparations.

26 reptococcus pyogenes hyaluronate synthase in membrane preparations.

27 the activity detected in mitochondrial inner membrane preparations.

28 ecific binding protein present in cerebellar membrane preparations.

29 detected a single band at 46 kDa in similar membrane preparations.

30 resent at high concentrations in solubilized membrane preparations.

31 entrifugation to obtain endosomal and plasma membrane preparations.

32 fic proteins in PC-3 cell lysates and plasma membrane preparations.

33 not be immunochemically detected in platelet membrane preparations.

34 p of homogeneous noninteracting sites in all membrane preparations.

35 H]spiperone (227 +/- 83 fmol/mg) in the same membrane preparations.

36 to the Kd value was 65% to 90% in the three membrane preparations.

37 tes, and its failure to be detected in crude membrane preparations.

38 inity binding of agonist in intact cells and membrane preparations.

39 ates from both total outer segment and crude membrane preparations.

40 R2A cleavage fragments observed in rat brain membrane preparations.

41 o pull down the a4 subunit from human kidney membrane preparations.

42 proteins were found on immunoblots of sperm membrane preparations.

43 vine retina-retinal pigment epithelial (RPE) membrane preparations.

44 pholipid lysophosphatidylcholine (LPC), from membrane preparations.

45 ct primary human keratinocytes and cell-free membrane preparations.

46 ate proteoglycan (CSPG) on neutrophil plasma membrane preparations.

47 ance (EPR) spectroscopic analysis of ordered membrane preparations.

48 ants, but it inactivated beta-CIT binding in membrane preparations.

49 expressed in whole-cell lysates and in cell membrane preparations.

50 fected HEK-293 cells, and rat brain synaptic membrane preparations.

51 increased solubilization of COH1 from lipid membrane preparations.

52 sented inner membrane contamination of outer membrane preparations.

53 naling through adenylyl cyclase (AC) in cell membrane preparations 4 h later.

54 In both intact yeast cells and membrane preparations, agonist does not affect FRET effi

55 When isolated in cellular membrane preparations, AIR was found to be capable of au

56 Heat-killed mycoplasmas or mycoplasmal membrane preparations alone could support continued grow

57 eins adjacent to the choroidal margin of the membrane preparation and a saline solution adjacent to t

58 as solubilized from a highly enriched plasma membrane preparation and purified by Ni2+-chelating chro

59 the maximum binding sites (Bmax) in a bovine membrane preparation and similar rabbit fraction were 0.

60 Using membrane preparations and a complete cascade of purified

61 2 and 4 can be readily detected in platelet membrane preparations and are shown to participate in 20

62 stimulated adenylyl cyclase (AC) activity in membrane preparations and did so primarily by altering t

63 FIC1 cDNA resulted in appearance of FIC1 in membrane preparations and energy-dependent PS translocat

64 activity in RG20 cells, as assessed both in membrane preparations and in intact cells.

65 lysates from transgenic mouse brain cortical membrane preparations and isolated a number of previousl

66 six negative strains expressed NspA in outer membrane preparations and since their predicted NspA ami

67 Using membrane preparations and the complete cascade of purifi

68 says showed immunoreactivity in yeast plasma membrane preparations, and a rho 1-GFP fusion gene showe

69 rease in cAMP production in adipocyte plasma membrane preparations, and pretreatment of cells with pe

70 that the ORF113 protein was present in outer membrane preparations, and this protein was also shown t

71 ement and applicable to the isolated enzyme, membrane preparations, and tissue homogenates.

72 mer formation occurring via oxidation during membrane preparation as well as for dimer cross-linking

73 nce the [(35)S]GTPgammaS assay uses the same membrane preparations as the binding assay, differential

74 es generated by incubating Tf-labeled plasma membrane preparations at 37 degreesC.

75 ssion of GPR40-Galpha(q) in HEK293 cells and membrane preparation basal binding of [(35)S]GTPgammaSin

76 e plasmid into a C. coli strain lacking CDT, membrane preparations became positive in both CDT and IL

77 etabolism inhibitors blocked MOMP from heavy membrane preparations but failed to influence MOMP in th

78 brane was permeabilized with digitonin or in membrane preparations but not in intact cells.

79 The cross-linking reactions of the membrane preparation, but not those of the solubilized o

80 can be rapidly processed and activated from membrane preparations by caspase-1 without detergents.

81 (+)-ATPase activity was measured in cortical membrane preparations by determining the rate of ATP hyd

82 atidine displacement in intact M10 cells and membrane preparations by membrane-impermeant ligands ind

83 Plasma membrane preparations can be made in 15 min.

84 Western analysis of membrane preparations confirmed the observations on mRNA

85 Microsomal membrane preparations contained delta9, delta12 and delt

86 Mutant membrane preparations contained increased amounts of the

87 Exposure of membrane preparations containing an inactive RER-localiz

88 Furthermore, membrane preparations containing EGFR can bind to GM3-co

89 GPCR arrays were obtained by printing membrane preparations containing GPCRs using a quill-pin

90 Enzymatic deglycosylation of the membrane preparations converted both immunoreactive prot

91 ethroid binding to Na+ channels in rat brain membrane preparations could be measured and reached 75%

92 ron paramagnetic resonance in a native outer-membrane preparation demonstrate that signaling also occ

93 by infection with a recombinant baculovirus; membrane preparations derived from the infected Sf9 cell

94 Moreover, deglycosylation of HepG2 membrane preparations did not affect either HDL binding

95 Expression of ordA in a yeast membrane preparation enabled the intermediacy of HOMST b

96 Membrane preparations enriched in plasma membrane vesicl

97 riments showed that purified synaptic plasma membrane preparations enriched in PrP(C) but largely dep

98 rage Kd values for the various rat and mouse membrane preparations examined were 4.2 +/- 0.7 nM and 3

99 ies were in complete agreement with caveolar membrane preparation findings.

100 ation and differential centrifugation into a membrane preparation for in vitro measurement of cardiac

101 Ligand blotting assays of kidney membrane preparations fractionated by SDS-PAGE revealed

102 EGF-stimulable protein tyrosine kinase in a membrane preparation from A431 cells was inactivated by

103 In membrane preparation from control brainstem tissues, Wes

104 in vitro arabinosyltransferase assay using a membrane preparation from M. smegmatis expressing Rv3792

105 a-opioid receptor proteins were expressed in membrane preparation from morphine-tolerant rats.

106 ion, a highly complex peptide mixture (outer membrane preparation from Psuedemonas aeruginosa) was ef

107 Heavy membrane preparations from 697 lymphoblastoid cells cont

108 Membrane preparations from 81-176 mutants defective in a

109 embrane protein, porin A (PorA), no PorA and membrane preparations from a mutant with no LPS (LpxA(-)

110 We have similarly studied the semiquinone in membrane preparations from a strain with overexpression

111 Plasma membrane preparations from activated monocytes also indu

112 The sigma1 affinity was tested using membrane preparations from animal (guinea pig) and human

113 ransmitter receptor agonists was measured in membrane preparations from both groups.

114 e-polyacrylamide gel separations of enriched membrane preparations from bovine and human lenses.

115 activity and [Ca2+] was shifted rightward in membrane preparations from cardiomyocytes infected with

116 Highly washed membrane preparations from cells of the hyperthermophili

117 ve studied NarX autophosphorylation in crude membrane preparations from cells that overexpress NarX p

118 mannan biosynthesis in vitro is catalysed by membrane preparations from developing fenugreek seed end

119 pecified by FKS1 and FKS2 as demonstrated in membrane preparations from fks2 and fks1 deletion strain

120 Irradiated cells or membrane preparations from fresh or frozen tumor tissue

121 and 0.82) with IC(50) values using receptor membrane preparations from Helix aspersa.

122 In crude membrane preparations from HOS cells, a higher level of

123 Analysis of enriched mitochondrial membrane preparations from human cells yielded identific

124 In vitro studies in isolated membrane preparations from insect cells infected with MD

125 members of the Src family (c-Src, c-Fyn) and membrane preparations from keratinocytes did.

126 In membrane preparations from mammalian cells, genistein is

127 adenylyl cyclase activity in purified plasma membrane preparations from N18TG2 neuroblastoma cells.

128 A)-photoaffinity labelled in vitro in plasma membrane preparations from oat (Avena sativa L.) aleuron

129 strate that the ratio of MT1-MMP to TIMP2 in membrane preparations from PAF-stimulated HUVEC is 1.6:1

130 tors are detected in intestinal brush border membrane preparations from pigs with adhesive phenotypes

131 and glycerol permeabilities in mitochondrial membrane preparations from rat brain, liver, and kidney

132 y was performed in 96-well microplates using membrane preparations from rat liver as a source of ASGP

133 Experiments were performed in PSII membrane preparations from spinach in the presence of el

134 The binding of [3H]muscimol to membrane preparations from the brain of the bullfrog, Ra

135 was detectable by the fluorographic assay in membrane preparations from the mutants, and comparison o

136 agonists and the CysLT1 receptor has been in membrane preparations from tissues enriched for this rec

137 methanethiosulfonate hydrobromide (MTSEA) in membrane preparations from transfected cells.

138 nct protein-tyrosine phosphatase (PTPase) in membrane preparations from v-Ras transformed NIH 3T3 cel

139 l saturable high and low affinity binding to membrane preparations from wild-type mice, but not to pr

140 Membrane preparations from young donors were permeable t

141 nsight into the scramblase activity in crude membrane preparations, functional validation of candidat

142 s (Sec23/24p, Sec13/31p, and Sar1p) to yeast membrane preparations generated vesicles containing the

143 ately 3.3% of the total) suggests the plasma membrane preparation has minimum contamination from thes

144 ctin ligand activity on leukocytes, an HL-60 membrane preparation immunodepleted of PSGL-1 supported

145 Using a membrane preparation in which both extracellular and int

146 radykinin for binding to dog skeletal muscle membrane preparations in a biphasic manner.

147 ith NECA increased the pp1 activity of crude membrane preparations in a time- and dose-dependent fash

148 the wild-type receptor declined over time in membrane preparations in vitro, and this loss was blocke

149 e formation in vivo and detected activity in membrane preparations in vitro.

150 S myelin that is not shared by several other membrane preparations including adult and neonatal neura

151 Ca2+ uptake assays in a membrane preparation indicated a 3-4-fold increase in Ca

152 for the stable cell line, and titration of a membrane preparation indicated a K(d) value of 0.8 nM.

153 d from the affinity-enriched integral plasma membrane preparation led to the identification of 898 un

154 In phosphorylation assays with pollen membrane preparations, LePRK2, but not LePRK1, is phosph

155 fic C12:0 3-OH and C14:0 3-OH present in the membrane preparations (MP) from NMBA11K3 were substantia

156 Furthermore, in crude membrane preparations, neither the basal nor the prazosi

157 serves as the site of covalent attachment, a membrane preparation of labeled receptor was subjected t

158 he existence of a dipeptide transporter in a membrane preparation of liver lysosomes using Gly-3H-Gln

159 A membrane preparation of the photolabeled receptors was a

160 lyses of sigma 2 receptors were performed on membrane preparations of 66 P cells from 3-day cultures

161 Membrane preparations of A. fermentans contain a highly

162 AC toxin was extracted from crude outer membrane preparations of B. pertussis with 8 M urea, but

163 se high-performance liquid chromatography to membrane preparations of beta-cell tumors.

164 ximately 43-kDa protein exclusively in outer membrane preparations of both pathogens.

165 netic, saturation, and competition assays at membrane preparations of Chinese hamster ovary cells rec

166 erine racemase activity was also detected in membrane preparations of constitutively vancomycin-resis

167 amiprilat was used to measure ACE protein in membrane preparations of hearts obtained from 36 subject

168 oradiolabeling experiments were conducted in membrane preparations of HEK293 cells overexpressing mou

169 shown that the major AAV2 binding protein in membrane preparations of human cells corresponds to a gl

170 Membrane preparations of human CYP4B1(Pro) and rabbit CY

171 s (inhibition constant = 0.081 nmol/L, using membrane preparations of LLC-PK1 cells expressing the sp

172 Membrane preparations of MIG1 were enriched in two glyco

173 se activity could be obtained from cell-free membrane preparations of P. multocida.

174 [3H]P1075 binding to membrane preparations of rabbit skeletal muscle were obs

175 annose to endogenous polyprenol phosphate in membrane preparations of S. coelicolor.

176 Using membrane preparations of S. pneumoniae in an in vitro as

177 epsilon-sarcoglycan was also reduced in membrane preparations of striated and smooth muscle.

178 Membrane preparations of unactivated (Rh) and light-acti

179 ures of E. coli expressing gly1 and in outer membrane preparations of wild-type N. gonorrhoeae.

180 In this study, proteins present in the outer membrane preparation (OMP) of four H. bilis strains isol

181 We fractionated mitochondrial membrane preparations on flotation gradients and show th

182 elta 7-V5) was detected in Ni-resin-purified membrane preparations only when coexpressed with a histi

183 with islets, isolated islet cells, or islet membrane preparations, others have known antigen specifi

184 thapsigargin inhibition in several reticular membrane preparations, our results suggest that luminal

185 ed as the quantity of protein traversing the membrane preparation per unit area.

186 ging their affinity for DAT when measured in membrane preparations prepared from these cells.

187 d via Scatchard analysis on a series of PSII membrane preparations progressively depleted of the extr

188 In membrane preparations, PTPIB complexed with tyrosine pho

189 binding proteins are labeled in solubilized membrane preparations; reduction in size of the 230 kDa

190 receptor per mg of protein in the extracted membrane preparations, representing a 2- to 3-fold enric

191 rays use approximately 200- to 400-fold less membrane preparation required by conventional assay meth

192 omato style, but not leaf, extracts to these membrane preparations results at least partially in spec

193 between active site-directed spin-labels in membrane preparations show excellent agreement with thos

194 Plasma membrane preparations showed that OR51E2 protein is pres

195 SDS-PAGE of the three ROS membrane preparations showed that they were of identical

196 In mouse brain membrane preparations, sigma(1)-selective antagonists al

197 monstrated that each of these photosystem II membrane preparations strongly bound two copies of the 3

198 and for other protein components in Torpedo membrane preparations, such as RAPsyn and Na(+)-K(+)-ATP

199 ctable GalA transferase activity in isolated membrane preparations, suggesting that the appropriate G

200 ty of SERCA1 and its affinity for calcium in membrane preparations suitable for structural analysis b

201 5-OxoETE also specifically bound to the membrane preparations that conducted GTP/GDP exchange.

202 With the plasma membrane preparation, the presence of large amounts of s

203 In membrane preparations, the beta(3b)-AR activated Galpha(

204 showed low activity and low abundance in the membrane preparations, the deletion mutants were able to

205 In platelet membrane preparations, the peptide agonists stimulated a

206 ly patterned agarose gels to deliver various membrane preparations to glass substrates in a rapid and

207 d membrane subsets and detergent-solubilized membrane preparations to support PrPres amplification.

208 ties of (+/-)-[3H]epibatidine in spinal cord membrane preparations to the cardiovascular and behavior

209 endothelial cells, and rat lung cytosol and membrane preparations; to detect them we have applied co

210 easurement of the number of receptors in the membrane preparation used, the relative effectiveness of

211 Western blot analyses of the same membrane preparations used for the electrophysiological

212 procaspase can be efficiently liberated from membrane preparations using detergents.

213 pMMO can be subsequently obtained from these membrane preparations using protocols in which an excess

214 RC-160 to receptors for SST on rat pituitary membrane preparation was also determined.

215 eceptor-mediated activation of G proteins in membrane preparations was blocked by cell pretreatment w

216 ing of [(3)H]resiniferatoxin (RTX) in plasma membrane preparations was inhibited by CGS21680, an A(2A

217 site-directed spin labeling of intact outer membrane preparations was used to investigate the confor

218 chamber cultures and activated T cell plasma membrane preparations we demonstrated that both cell con

219 Using a cell-free, lysed synaptosomal membrane preparation, we show that Ca2+ alone is suffici

220 In this study, using purified carp cone membrane preparations, we first confirmed that the react

221 The properties of each membrane preparation were assessed by biochemical and ph

222 Proteins present in the membrane preparation were reduced, alkylated, and cleave

223 reatments on HCLL activity of KG1a cells and membrane preparations were analyzed.

224 Membrane preparations were examined for their sensitivit

225 Enriched sarcolemmal membrane preparations were found to possess ATP.AMP phos

226 alysis revealed that 75% of the receptors in membrane preparations were functional; there was little,

227 ists in the mature erythrocyte membrane, RBC membrane preparations were immunoblotted with antiserum

228 Bruch's membrane preparations were isolated from the macular reg

229 ux in wheat (Triticum aestivum), root plasma membrane preparations were screened using the planar lip

230 sed in mammalian COS cells and the resulting membrane preparations were treated with peptide N-glycos

231 Crude Sf9 cell membrane preparations were used to show that the purport

232 xperiments showed that Shu1 is released from membrane preparations when spheroplast lysates are incub

233 uR2/3 subunits of AMPA receptors in synaptic membrane preparations, whereas no change was observed in

234 mined the rebinding of this protein to PS II membrane preparations which contain four, two, or zero m

235 um (R24571) was added to the isolated plasma membrane preparation, which lowered the (Ca(2+)+Mg(2+))-

236 ociated with mature P. falciparum gametocyte membrane preparations, which is dependent on the presenc

237 l antibody, C5/D5, raised against epithelial membrane preparations, which markedly inhibits PMN migra

238 In a mouse hippocampal membrane preparation with [131I](R)-8 as radioligand, ra

239 laced [3H]CP-55940 binding to a rat brain P2 membrane preparation with an IC50 of 690 nM, which was 1

240 Cysteine-substitution at H278 yields membrane preparations with greatly decreased receptor de

241 s bound to the RLF receptor in uterine crude membrane preparations with high affinity (73 nM for (125