ライフサイエンスコーパス: mesophilic

1 ted Ascaris suum ova in six laboratory-scale mesophilic (35 degrees C) anaerobic digesters processing

2 bed reactors (51-56 degrees C) followed by a mesophilic (36.5 degrees C) anaerobic filter.

3 Escherichia coli but showed no similarity to mesophilic ACS-type enzymes.

4 ere able to design more stable variants of a mesophilic adenylate kinase with only the sequence infor

5 this is analogous to previous findings with mesophilic ADH at 25 degrees C.

6 l tables for the reference identification of mesophilic aeromonads.

7 ich are soil-transmitted human pathogens, in mesophilic anaerobic digestion processes.

8 er of resource recovery technologies such as mesophilic anaerobic digestion to developing world setti

9 technologies (air drying, aerobic digestion, mesophilic anaerobic digestion, thermophilic anaerobic d

10 ately more activated by temperature than the mesophilic analog.

11 DeltaDeltaGu = 6.9 kcal/mol) compared to its mesophilic analogue CenAP30.

12 rmophile Pyrococcus furiosus (PfRd), and its mesophilic analogue Clostridium pasteurianum (CpRd), are

13 Although most eukaryotic TBPs are mesophilic and adapted to physiological conditions of te

14 the chromogenic data with concentrations of mesophilic and Enterobacteriaceae.

15 he primary mode of salt stabilization of the mesophilic and halophilic DHFRs appears to be through pr

16 l system to compare the effect of salts on a mesophilic and halophilic enzyme.

17 ce in the salt-dependent binding behavior of mesophilic and halophilic TBPs to DNA may be due to the

18 ally observed salt-dependent DNA-binding for mesophilic and halophilic TBPs, and suggest that the dis

19 ral kinetic steps, and to differ between the mesophilic and hyper-thermophilic proteins.

20 The fast, local mobilities differ between a mesophilic and hyperthermophilic adenylate kinase, but a

21 rative analysis of genome sequence data from mesophilic and hyperthermophilic micro-organisms has rev

22 Because POP is found in mesophilic and hyperthermophilic organisms, and is distr

23 reover, the single-subunit pyruvate ORs from mesophilic and moderately thermophilic bacteria and from

24 aeolicus (Aacpn10) shows high homology with mesophilic and other thermophilic cpn10 sequences, excep

25 prokaryotes that live at lower temperatures (mesophilic and psychrophilic Archaea and Bacteria).

26 tomic structures with citrate synthases from mesophilic and psychrophilic organisms has indicated the

27 From a pair of highly homologous mesophilic and thermophilic adenylate kinases, we genera

28 first obtained evidence that four different mesophilic and thermophilic archaeal RNase P holoenzymes

29 e evolutionary history between RNases H from mesophilic and thermophilic bacteria.

30 This configuration was tested at both mesophilic and thermophilic conditions.

31 The discovery of ammonia oxidation by mesophilic and thermophilic Crenarchaeota and the widesp

32 bases are incorporated into DNA by selected mesophilic and thermophilic DNA polymerases and the resu

33 or real-time single-molecule measurements of mesophilic and thermophilic enzymes at 70 degrees C.

34 agreement with other comparative studies of mesophilic and thermophilic enzymes.

35 ampled locations in the lake, including both mesophilic and thermophilic habitats, had multiple virop

36 itionally, comparisons between corresponding mesophilic and thermophilic motif pairs provide key bioc

37 ermined through global sequence alignment of mesophilic and thermophilic motif pairs, which are ident

38 e find that mitochondrial carriers from both mesophilic and thermophilic organisms exhibit poor stabi

39 ic homologue to the previously characterized mesophilic and thermophilic pair.

40 Pairwise comparisons of homologous mesophilic and thermophilic proteins can help to identif

41 t differences in packing density between the mesophilic and thermophilic proteins.

42 ight-harvesting antennae can be regulated in mesophilic and thermophilic red algae.

43 cts influence the unfolding kinetics of both mesophilic and thermophilic rubredoxins, these findings

44 proach to identify and compare corresponding mesophilic and thermophilic sequence motifs between all

45 ed with homologous enzymes from a variety of mesophilic and thermophilic sources.

46 ility of the patties, delaying total aerobic mesophilic, and lactic acid bacteria growth, especially

47 e hot-start toward modern hyperthermophilic, mesophilic, and psychrophilic organisms illustrates acti

48 bacteria, that also occur in some species of mesophilic archaea and in the endosymbiotic organelles o

49 at shock regulation in hyperthermophilic and mesophilic Archaea organisms.

50 is a dimeric enzyme in eucarya, bacteria and mesophilic archaea.

51 p (PCNA) and the clamp loader (RFC) from the mesophilic archaeon Methanosarcina acetivorans.

52 Methanococcus maripaludis is a mesophilic archaeon that reduces CO2 to methane with H2

53 to KGOR has been previously purified from a mesophilic archaeon, but the molecular properties of T.

54 re also homologous to the dimeric POR from a mesophilic archaeon, Halobacterium halobium (21% identit

55 nother well characterized AroQ class CM, the mesophilic AroQp domain from E. coli.

56 HB8 DNA ligase (Tth DNA ligase) differs from mesophilic ATP-dependent DNA ligases in three ways: (i)

57 cold shock protein (Bc-Csp) differs from the mesophilic Bacillus subtilis cold shock protein B (Bs-Cs

58 nd 1.38 log CFU/g reduction of total aerobic mesophilic bacteria (TAMB) counts.

59 tinguishes itself from nitrate reductases of mesophilic bacteria and archaea by its very high specifi

60 ated compounds and K(1)-value and microbial (mesophilic bacteria and Enterobacteriaceae) analyses wer

61 in these organisms is markedly lower than in mesophilic bacteria and eukaryotes.

62 On the tenth day of storage, total aerobic mesophilic bacteria and mould and yeast counts were stat

63 hilic archaea, two thermophilic bacteria, 17 mesophilic bacteria and two eukaryotic species were anal

64 composition to filamentous viruses infecting mesophilic bacteria but is distinguished by in vivo asse

65 ly, the sHSP confers a survival advantage on mesophilic bacteria by preventing protein aggregation at

66 occur in DNA polymerase III holoenzyme from mesophilic bacteria including delta-delta' interaction,

67 RlmO homologues are primarily found in mesophilic bacteria related to T. thermophilus.

68 st+mold during 12days of storage while total mesophilic bacteria was decreased during 6days of storag

69 contrast to many polymerization ATPases from mesophilic bacteria, ATP binding is not required for Pil

70 y developed Cas9 homologs all originate from mesophilic bacteria, making them susceptible to degradat

71 in contrast to replicative polymerases from mesophilic bacteria, Tth holoenzyme is efficient only at

72 mon to known peptide-signalling molecules in mesophilic bacteria, was strongly upregulated in the co-

73 It occurs in mesophilic bacteria, yeast, and thermophilic archaea.

74 Mesophilic bacteria, yeast, mold and pathogenic Enteroba

75 ing), which has previously been described in mesophilic bacteria.

76 a maritima, and those of close homologs from mesophilic bacteria.

77 hermophilic bacteria differ from the rRNA of mesophilic bacteria.

78 is waterline contamination by heterotrophic mesophilic bacteria.

79 MalQ represents the first example of a mesophilic bacterial amylomaltase with known structure a

80 he hyperthermophilic archaeal enzyme and the mesophilic bacterial enzyme, structural modifications mu

81 nt in Escherichia coli RNase R and in 88% of mesophilic bacterial genera analyzed, but absent from th

82 same relative order and spacing found in the mesophilic bacterial proteins.

83 Mesophilic bacterial proteomes contain a small number (0

84 characteristics of the conserved domains of mesophilic bacterial response regulators, and the C-term

85 ic archaeon Pyrococcus furiosus (Pf) and the mesophilic bacterium Clostridium pasteurianum (Cp) were

86 ophilic archaeon Pyrococcus furiosus and the mesophilic bacterium Clostridium pasteurianum.

87 eesC for the beta-glucosidase (abg) from the mesophilic bacterium, Agrobacterium faecalis.

88 vestigated the growth of Escherichia coli, a mesophilic bacterium, as a function of pressure (P) and

89 te synthase (AS) from Serratia marcescens, a mesophilic bacterium, has been solved in the presence of

90 e transport proteins shown to be active in a mesophilic bacterium.

91 values) dynamics for a continually operated mesophilic bioreactor and highlight the enormous potenti

92 requency (44%) than those generated from the mesophilic BsAK (6%), and selected TnAK mutants compleme

93 Why do mesophilic cells die around 50 degrees C?

94 of Clostridium cellulovorans, an anaerobic, mesophilic, cellulolytic bacterium, was characterized.

95 the cold-active trypsin into a variant with mesophilic characteristics without changing the amino ac

96 eld observations of relative abundances: the mesophilic clade grows optimally at temperatures 16 degr

97 cum and C. josui, they seem to be typical of mesophilic clostridia, indicating that the large gene cl

98 in, rubredoxin (MW = 7500 Da), from both the mesophilic Clostridium pasteurianum (Topt = 37 degrees C

99 en in simulations of the reduced form of the mesophilic Clostridium pasteurianum rubredoxin at 295 K,

100 rthermore, the samples from thermophilic and mesophilic codigesters had different DOM composition in

101 otein, CspB-TB that has the same core as the mesophilic cold shock protein CspB-Bs from Bacillus subt

102 unfold a homologous pair of thermophilic and mesophilic cold shock proteins at high temperatures.

103 drothermal conditions, is precipitated under mesophilic conditions by the metabolically versatile str

104 g arsenic-bearing sediments under anaerobic, mesophilic conditions in minimal media with acetate as t

105 this energetic pathway has not been shown in mesophilic conditions.

106 od to alleviate ammonia toxicity effect in a mesophilic continuously stirred-tank reactor (CSTR) oper

107 pproximately 5 kcal/mol more stable than its mesophilic counterpart as judged from equilibrium denatu

108 ns, which unfold much more slowly than their mesophilic counterparts, T.thermophilus RNase H folds an

109 al peptide motifs that are absent from their mesophilic counterparts.

110 more compact and more hydrophobic than their mesophilic counterparts.

111 ic organisms are often more rigid than their mesophilic counterparts.

112 ally in a fundamentally different way to its mesophilic counterparts.

113 res and greater thermosensitivity than their mesophilic counterparts.

114 lity for thermophilic enzymes than for their mesophilic counterparts.

115 comparison of themophilic enzymes with their mesophilic counterparts.

116 ound were: (1) a disulfide bond not found in mesophilic counterparts; (2) an increased number of surf

117 ch other and close to reported midpoints for mesophilic cpn10 proteins.

118 d to the ancestral precursor of contemporary mesophilic CPSases.

119 kinetics and cellular characteristics of the mesophilic crenarchaeon 'Candidatus Nitrosopumilus marit

120 the reaction center and antenna system from mesophilic cyanobacteria, including red chlorophylls and

121 s between thermophilic Thermus aquaticus and mesophilic Deinococcus radiodurans RNAPs and identify th

122 secondary treatment and the later anaerobic mesophilic digestion of the sludge.

123 Five DNA polymerases were investigated: mesophilic DNA polymerase I large (Klenow) fragment, 3'-

124 thermophilus ribonuclease H compared to its mesophilic E. coli homolog.

125 spectrometry (HDX-MS) has been applied to a mesophilic (E. coli) dihydrofolate reductase under condi

126 report the characterization of two different mesophilic EF-Tu orthologs, one from Escherichia coli, a

127 suggested by a comparison with a homologous mesophilic enzyme (55% identity), NAD(+)-dependent alcoh

128 ntersubunit linkages of the thermophilic and mesophilic enzyme Bacillus subtilis chorismate mutase su

129 nfolding (DeltaSu) of 0.55 kcal/(mol*K); the mesophilic enzyme CenAP30 had a Tm of 56.4 degrees C, a

130 ydrofolate reductases, namely the monomeric, mesophilic enzyme from E. coli (EcDHFR) and the dimeric,

131 inding and activity less frequently than the mesophilic enzyme, although these differences may manife

132 mately eight times higher than that based on mesophilic enzymes at the same temperature.

133 al similarity between this cellulase and the mesophilic enzymes serves to highlight features that may

134 obiose and beta-1-galactosylphingosine) with mesophilic enzymes.

135 aptive role in catalysis by thermophilic and mesophilic enzymes.

136 nit displayed much higher stability than the mesophilic equivalent and its iron-sulfur cluster remain

137 er promoter escape efficiency than RNAP from mesophilic Escherichia coli.

138 tionship between stability and activity in a mesophilic esterase.

139 ryotic organisms, including thermophilic and mesophilic eubacteria as well as archaebacteria, the hum

140 Comparison of these structures with those of mesophilic family 12 cellulases in complex with inhibito

141 arison of the structure of Tl Fd to those of mesophilic ferredoxins reveals that Tl Fd possesses the

142 enhanced thermostability of PH75 relative to mesophilic filamentous bacteriophages.

143 with the same symmetry (class II) as in the mesophilic filamentous phages Pf1 and Pf3.

144 nt is not apparent in capsid subunits of the mesophilic filamentous phages, fd, Pf1, and Pf3, previou

145 Previously, a mesophilic form of HDA (mHDA) utilizing the Escherichia

146 dominant (per)chlorate-reducing bacteria in mesophilic freshwater environments.

147 The most abundant beta-glucosidase in the mesophilic fungus Hypocrea jecorina is HjCel3A, which hy

148 urpassed the conventional system utilizing a mesophilic G6PDH (mG6PDH) from Leuconostoc mesenteroides

149 and V482L) in closely related homologs from mesophilic haloarchaea.

150 Methylomonas methanica MC09 is a mesophilic, halotolerant, aerobic, methanotrophic member

151 different for hyperthermophilic (IPPase) and mesophilic (HEWL) proteins.

152 The mesophilic hFoB is very unstable and requires approximat

153 For the mesophilic HLADH described herein, an opposite trend is

154 As in mesophilic holoenzymes, in the presence of a primed DNA

155 e from Thermus thermophilus, compared to its mesophilic homolog from Escherichia coli, is elucidated

156 and close to half that of its highly similar mesophilic homolog, subtilisin SSII, indicating that the

157 yperthermostable protein was compared to its mesophilic homologs and analyzed for differences in the

158 several hyperthermophilic proteins and their mesophilic homologs are calculated.

159 d to survive extreme temperatures with their mesophilic homologs are likely to provide valuable infor

160 ophilic sequences are more likely than their mesophilic homologs to have deletions in exposed loop re

161 s are generally more thermostable than their mesophilic homologs, but little is known about the evolu

162 ened thermophilic proteins relative to their mesophilic homologs.

163 m folding of a thermophilic ribozyme and its mesophilic homologue by using hydroxyl radical protectio

164 enobacter thermophilus (Ht cyt c552) and its mesophilic homologue from Pseudomonas aeruginosa (Pa cyt

165 iors of the ion pairs when engineered into a mesophilic homologue to increase stability, in silico mu

166 ng temperature (T(m)) similar to that of the mesophilic homologue yet also has a surprisingly low Del

167 The mesophilic homologue, BBL, was less stable than the ther

168 compared to previously published data for a mesophilic homologue, Escherichia coli ribonuclease HI (

169 When it is compared with its mesophilic homologue, L30e from yeast, a number of struc

170 ermophilic protein is similar to that of its mesophilic homologue, with a proportional increase in st

171 three hyperthermophilic proteins with their mesophilic homologues and find that hydration effects pr

172 teins are significantly higher than those of mesophilic homologues at 30 degrees C; thus, heptamer th

173 ns are significantly more compact than their mesophilic homologues, while no particular interaction t

174 show pronounced structural differences from mesophilic homologues.

175 gies of mutant cores based on cores found in mesophilic homologues.

176 are considerably greater than those of their mesophilic homologues.

177 onal thermodynamics between thermophilic and mesophilic homologues.

178 proteins are very similar to those of their mesophilic homologues.

179 ydrogenase (HtADH) closely resembles that of mesophilic horse liver alcohol dehydrogenase (HLADH).

180 een cloned and expressed at high levels in a mesophilic host (Escherichia coli) as a soluble tetramer

181 genes encoding hyperthermophilic enzymes in mesophilic hosts have improved the availability of high-

182 enome sequence of the genetically tractable, mesophilic, hydrogenotrophic methanogen Methanococcus ma

183 ed wild type cellobiohydrolases (Cel7A) from mesophilic Hypocrea jecorina and thermophilic Rasamsonia

184 replacements allowed us to develop the first mesophilic in vitro protein splicing system as well as s

185 led us to demonstrate in vitro splicing of a mesophilic intein containing all wild-type catalytic res

186 olII intein is significantly more rigid than mesophilic inteins, which may contribute to the higher o

187 hat required for equivalent fermentations by mesophilic lactic acid bacteria.

188 Ts with more than 5 rings, in sediments from mesophilic marine environments (sea surface temperature,

189 euryarchaeote Methanococcus maripaludis is a mesophilic member of the Sac10b family.

190 d has identical topology to those of the two mesophilic members of the family whose structures have b

191 fferential features for distinguishing other mesophilic members of the genus.

192 from thermoadapted homologs into an exemplar mesophilic membrane protein, and demonstrated significan

193 ene isolation, while in vivo genetics in the mesophilic methanococci can provide the experimental sys

194 that the physiological role of Mma10b in the mesophilic methanococci is greatly diverged from that of

195 aschii are compared with their homologs from mesophilic Methanococcus species.

196 ore the function of all three enzymes in the mesophilic methanogen Methanosarcina mazei.

197 stress genes in the hsp70(dnaK) locus of the mesophilic, methanogenic archaeon Methanosarcina mazeii.

198 ophilic archaea and that the polymerase from mesophilic Methanosarcina acetivorans shows identical be

199 was lower than corresponding estimates from mesophilic microorganisms, primarily because of a low ra

200 ve been measured for enzymes from a range of mesophilic microorganisms.

201 a coli cytoplasmic membrane and membranes of mesophilic microorganisms.

202 ilic model organism distantly related to the mesophilic model organism E. coli.

203 plays much faster relaxation dynamics than a mesophilic model protein, hen egg white lysozyme (HEWL),

204 g for the t+rRNA modification enzymes in the mesophilic moderate halophile Haloferax volcanii.

205 t a given temperature than the corresponding mesophilic (Ms) enzymes, because the thermophilic enzyme

206 gnal typical of the so-called Ni-C center of mesophilic NiFe-hydrogenases.

207 Methylophaga thiooxydans is a mesophilic, obligately halophilic bacterium that is capa

208 ins were extant proteins from psychrophilic, mesophilic, or thermophilic organisms.

209 rences in dynamics between homologs from the mesophilic organism E. coli and the thermophilic organis

210 This is the first report of a single mesophilic organism that can grow while catalyzing the o

211 essential part of genome per replication for mesophilic organisms and one to two mutations per genome

212 entical to those of corresponding mutants of mesophilic organisms encompassing a broad phylogenetic r

213 (CPSase) resembling the enzyme found in all mesophilic organisms nor a carbamate kinase-like CPSase

214 ant similarity with those of prolidases from mesophilic organisms, but the enzyme differs from them i

215 very similar to rpsL mutations identified in mesophilic organisms.

216 te data obtained in a dozen thermophilic and mesophilic organisms.

217 ly distant F-type motors of thermophilic and mesophilic origins, and they differ only in the magnitud

218 a to state transitions were observed only in mesophilic P. cruentum with mobile phycobilisomes, and t

219 origin of its stability by comparing it with mesophilic P450s with known structures.

220 its closest phylogenetic neighbor Frankia, a mesophilic plant endosymbiont and soil dweller.

221 Here, using novel genome data from the mesophilic Porphyridium cruentum and Calliarthron tuberc

222 In mesophilic prokaryotes, the DNA-binding protein HU parti

223 s H that display the desired thermophilic or mesophilic properties, as defined by their DeltaC(p) val

224 tein (CspB-TB) that has the core residues of mesophilic protein from Bacillus subtilis(CspB-Bs) and a

225 ction under conditions that render a typical mesophilic protein inactive.

226 xcluded-volume effect, the resistance of the mesophilic protein to heat-induced unfolding increased i

227 icient method to confer thermostability to a mesophilic protein.

228 the folding of the thermophilic but not the mesophilic protein.

229 unusually high thermodynamic stability for a mesophilic protein.

230 Both thermophilic and mesophilic proteins are maximally stable around room tem

231 ource organisms, homologous thermophilic and mesophilic proteins have similar stabilities.

232 ndidates likely to confer thermostability to mesophilic proteins through optimization of surface elec

233 ilies containing homologous thermophilic and mesophilic proteins which show reversible two-state fold

234 acity of unfolding (DeltaC(p)) than found in mesophilic proteins, is emerging from the recent literat

235 e thermodynamic profiles of thermophilic and mesophilic proteins.

236 erature regimes, than RdCp and other typical mesophilic proteins.

237 pgraded to average CH4 content of 89% in the mesophilic reactor and 85% in the thermophilic.

238 ly prove phycobilisome mobility in two model mesophilic red alga strains, Porphyridium cruentum and R

239 ons have an important regulatory function in mesophilic red algae; however, in thermophilic red algae

240 We demonstrate that a rich mesophilic red algal gene repertoire is crucial for test

241 and the ultimate folding transitions in the mesophilic ribozyme become linked into a single transiti

242 hich one to three motifs of a 255-nucleotide mesophilic ribozyme were substituted with the correspond

243 Unlike the case of the mesophilic rubredoxin from Clostridium pasteurianum (RdC

244 states of hyperthermophilic (S16Thermo) and mesophilic (S16Meso) homologs of the ribosomal protein S

245 in the size of the active site loops at the mesophilic ScOMPDC and the thermophilic MtOMPDC.

246 The 19-residue phosphate gripper loop of the mesophilic ScOMPDC is much larger than the nine-residue

247 An extension of this approach to a mesophilic soil environment also reveals high levels of

248 omologous enzymes from hyperthermophilic and mesophilic sources.

249 Thus, the mesophilic species B. subtilis and E. coli share the sam

250 Methanococcus maripaludis is a mesophilic species of Archaea capable of producing metha

251 n extant thermophilic species than in extant mesophilic species, supporting the idea that the LUA liv

252 dsrA analyses and likely contributing to the mesophilic SRR measured.

253 we have used directed evolution to convert a mesophilic subtilisin-like protease from Bacillus sphaer

254 d-type and far surpasses those of homologous mesophilic subtilisins.

255 activation energy (100-160 kJ mol(-1)) than mesophilic sulfate reduction (30-60 kJ mol(-1)), which m

256 vibrio desulfuricans G20) is a Gram-negative mesophilic sulfate-reducing bacterium (SRB), known to co

257 ndent DNA-binding behavior of halophilic and mesophilic TBPs by using a combined molecular mechanics/

258 concentration, the opposite is observed for mesophilic TBPs.

259 ng activity of the T. thermophilus enzyme at mesophilic temperatures do so by reconfiguring the local

260 nd evaluated using short oligonucleotides at mesophilic temperatures.

261 l structure of CYP119 compared with the five mesophilic templates.

262 Recently the complete genomes for mesophilic, thermophilic and hyperthermophilic organisms

263 ree wild-type cold shock proteins taken from mesophilic, thermophilic, and hyperthermophilic bacteria

264 Here we examine mesophilic to thermophilic AOM in hydrothermal sediments

265 the same as the recognition sequence of the mesophilic Type II restriction endonuclease MaeII.

266 we describe the first genome sequence from a mesophilic, unicellular red alga, Porphyridium purpureum

267 rences in protein structure for enzymes from mesophilic vs thermophilic sources.

268 trace and its relative frequency of usage in mesophilic vs. thermophilic eubacteria.

269 ted denitrification temperature responses to mesophilic with concurrent community shifts, and anammox