ライフサイエンスコーパス: metabolic engineering

1 ly of protein complexes, trait stacking, and metabolic engineering.

2 ynthetic biology, developmental biology, and metabolic engineering.

3 an obstacle to progress in biotechnology and metabolic engineering.

4 r the future improvement of rice quality via metabolic engineering.

5 erate genetic transfers in biotechnology for metabolic engineering.

6 ynthesis that combines organic chemistry and metabolic engineering.

7 , target validation, protein production, and metabolic engineering.

8 ays as titratable expression systems and for metabolic engineering.

9 g plant productivity is an important aim for metabolic engineering.

10 production have been widely in use in modern metabolic engineering.

11 te the utilization of gene overexpression in metabolic engineering.

12 egulatory network, unveiling new targets for metabolic engineering.

13 ndulol and its derivatives in plants through metabolic engineering.

14 f applications including genome analysis and metabolic engineering.

15 valuable tool in both pathway discovery and metabolic engineering.

16 f microorganisms, as well as informing their metabolic engineering.

17 he context of directed evolution and inverse metabolic engineering.

18 rast to their prominent success in microbial metabolic engineering.

19 arked contrast to their prominent success in metabolic engineering.

20 computational-based rational design of plant metabolic engineering.

21 ions as diverse as environmental sensing and metabolic engineering.

22 such as prediction of gene essentiality and metabolic engineering.

23 , analysis of phenotypic characteristics and metabolic engineering.

24 mall molecule is one of the primary goals in metabolic engineering.

25 and grow with the rapidly changing field of metabolic engineering.

26 andidate fuel molecules that can be made via metabolic engineering.

27 reading frames provide useful resources for metabolic engineering.

28 ze the potential of TFs for predictive plant metabolic engineering.

29 the phenotypic effects of knock-outs and for metabolic engineering.

30 ht make them highly useful targets for plant metabolic engineering.

31 vo molecular reporters and gene switches for metabolic engineering.

32 ks in a system and potential applications to metabolic engineering.

33 from the allied fields of protein design and metabolic engineering.

34 crops through marker-assisted selection and metabolic engineering.

35 py or gene alteration in systems biology and metabolic engineering.

36 nd the key differentiating characteristic of metabolic engineering.

37 ript stability, are essential in chloroplast metabolic engineering.

38 its activity, thereby coupling molecular and metabolic engineering.

39 lation applications such as gene therapy and metabolic engineering.

40 ctories, making them interesting targets for metabolic engineering.

41 considered for high-throughput screening in metabolic engineering.

42 n and regulation of high-producer strains in metabolic engineering.

43 truction, community functional analysis, and metabolic engineering.

44 ns at the interface of synthetic biology and metabolic engineering.

45 ns of QS-regulation in synthetic biology and metabolic engineering.

46 ng gene sequencing for pathway discovery and metabolic engineering.

47 lux are therefore critical to the success of metabolic engineering.

48 x analysis, which is an emerging strategy in metabolic engineering.

49 The results have potential applications in metabolic engineering.

50 f genetic manipulation of C. reinhardtii for metabolic engineering.

51 crop platforms and emerging technologies for metabolic engineering also hold promise for meeting glob

52 lators represent a largely untapped area for metabolic engineering and anti-bacterial development.

53 lexible and utilitarian chassis for advanced metabolic engineering and applied synthetic biology are

54 For different metabolic engineering and biotechnological applications,

55 C-MFA results with important applications in metabolic engineering and biotechnology.

56 including evaluation of network properties, metabolic engineering and drug discovery.

57 Recent work on metabolic engineering and experimental evolution of micr

58 biochemical pathways for synthetic biology, metabolic engineering and functional genomics studies.

59 s essential for improving the reliability of metabolic engineering and genome editing in undomesticat

60 o systematize and revolutionize the field of metabolic engineering and industrial biotechnology.

61 folia genome is a valuable resource for both metabolic engineering and molecular breeding.

62 strate and product sets, which is useful for metabolic engineering and prediction of nutritional requ

63 abolites is a tool of great significance for metabolic engineering and study of human disease.

64 A major challenge in metabolic engineering and synthetic biology is to balanc

65 Advances in metabolic engineering and synthetic biology will provide

66 Primarily used for metabolic engineering and synthetic biology, genome-scal

67 Through the careful application of metabolic engineering and synthetic biology, this biotra

68 ccharomyces cerevisiae using modern tools of metabolic engineering and synthetic biology-and the robu

69 luable compounds is a promising approach for metabolic engineering and synthetic biology.

70 int-based modeling has enabled the fields of metabolic engineering and systems biology to make great

71 genome analysis, basic research, education, metabolic engineering and systems biology.

72 review the current status and challenges of metabolic engineering and will discuss how new technolog

73 ased access (e.g., via molecular breeding or metabolic engineering) and enable reverse genetic approa

74 orporation of multiple CYPs into diterpenoid metabolic engineering, and a continuing trend of CYP pro

75 Now, advances in bioreactor technology, metabolic engineering, and analytical instrumentation ar

76 ency in the context of synthetic biology and metabolic engineering, and points to a promising future

77 expression systems for proteins, protein and metabolic engineering, and rational techniques for immob

78 to recent developments in genome sequencing, metabolic engineering, and synthetic biology.

79 ning lignin chemical depolymerization, plant metabolic engineering, and synthetic pathway reconstruct

80 ed the selection of genes to be modulated by metabolic engineering, and we demonstrate that the overe

81 and applying these methods to areas such as metabolic engineering, antibiotic design, and organismal

82 herein should be widely useful in a range of metabolic engineering applications in which essential en

83 le interest in exploiting bacterial MCPs for metabolic engineering applications, but little is known

84 onstrates the versatility of P. furiosus for metabolic engineering applications.

85 vity and potential utility of this mutant in metabolic engineering applications.

86 Here we present a metabolic engineering approach using Escherichia coli to

87 atural metabolic network, using a nonnatural metabolic engineering approach.

88 Metabolic engineering approaches are increasingly employ

89 In addition, metabolic engineering approaches for both the improvemen

90 Therefore, numerous metabolic engineering approaches have been attempted to

91 on metabolism are currently known, hindering metabolic engineering approaches to enhance productivity

92 These technologies now enable metabolic engineering approaches to optimize production

93 Metabolic engineering approaches will help to improve pr

94 is review, recent advances regarding terpene metabolic engineering are highlighted, with a special fo

95 purposes, new tools and methodologies within Metabolic Engineering are needed for the proposition and

96 f analogous techniques in the field of plant metabolic engineering are still in their infancy.

97 iology and technology that can be applied to metabolic engineering, are generating considerable excit

98 ygenic trait, and identifies new avenues for metabolic engineering as well as for construction of non

99 The integration of synthetic biology with metabolic engineering at the community level is vital to

100 ar metabolism) is of particular interest for metabolic engineering because it describes how carbon an

101 In the last decade, metabolic engineering benefited greatly from systems and

102 They hold great promise for metabolic engineering, but the behavior of plant metabol

103 perimental platform, we investigated whether metabolic engineering can be used to create syringyl lig

104 Plant genetics and metabolic engineering can be used to make foods that dif

105 Carotenoid metabolic engineering could enhance plant adaptation to

106 Our findings are relevant to metabolic engineering design and add to our understandin

107 and alleviates the primary bottleneck of the metabolic engineering design-build-test cycle.

108 comparative genomics, microbial diagnostics, metabolic engineering, drug design and analysis of metag

109 that modeling is a valuable tool for guiding metabolic engineering efforts aimed at improving essenti

110 This review also highlights metabolic engineering efforts aimed at increasing or dec

111 This information will facilitate metabolic engineering efforts aimed at producing medicin

112 review, we elucidate the recent progress in metabolic engineering efforts for the microbial producti

113 y that is an excellent model for exploratory metabolic engineering efforts into pathway regulation an

114 ays of these compounds is a prerequisite for metabolic engineering efforts that will improve producti

115 few years have seen a variety of interesting metabolic engineering efforts to improve the capabilitie

116 Here, we review recent metabolic engineering efforts to maximize production of

117 ust complex traits is a key challenge facing metabolic engineering efforts to synthesize valuable pro

118 r investigating cellular systems and guiding metabolic engineering efforts.

119 to specific gene functions and guide further metabolic engineering efforts.

120 nutrients in crops, which can be achieved by metabolic engineering, either using natural variation or

121 d in a variety of studies on drug discovery, metabolic engineering, evolution, and multi-species inte

122 Metabolic engineering exploits an integrated, systems-le

123 An ideal target for metabolic engineering, fatty acid biosynthesis remains p

124 explore some of these emerging applications--metabolic engineering for enhancing recombinant protein

125 s gene constructs to evaluate the utility of metabolic engineering for improving essential oil yield

126 e addressed, at least in part, through plant metabolic engineering for nutritional improvement of foo

127 We argue that metabolic engineering for producing the secondary metabo

128 Metabolic engineering for the overproduction of high-val

129 chnology applications, such as combinatorial metabolic engineering for the overproduction of secreted

130 DSDs have applications in metabolic engineering for the production of valuable pro

131 Our study illustrates the utility of metabolic engineering for the sustainable agricultural p

132 utility across multiple fields, for example metabolic engineering, growth phenotype simulation, and

133 erapeutic polyketide compounds, heterologous metabolic engineering has been applied to transfer polyk

134 As a discipline, Metabolic Engineering has provided a framework for the d

135 Metabolic engineering has the potential to produce from

136 Although microbial metabolic engineering has traditionally relied on ration

137 o liquid biofuels (e.g., bioethanol) through metabolic engineering have demonstrated potential for se

138 Recent advances in metabolic engineering have demonstrated that microbial b

139 Recent advances in metabolic engineering have demonstrated the potential to

140 Advances in molecular biology and metabolic engineering have provided new insights and tec

141 nthesis of volatile compounds have made this metabolic engineering highly feasible.

142 on technology have made this type of complex metabolic engineering highly feasible.

143 Multigene expression is required for metabolic engineering, i.e. coregulated expression of al

144 computational-based rational design of fish metabolic engineering in aquaculture.

145 ne function and modulate gene expression for metabolic engineering in microbes.

146 has been successfully applied as an aid for metabolic engineering in microorganisms.

147 Until recently, metabolic engineering in plants relied on the laborious

148 pathways are promising candidates for future metabolic engineering in R. opacus for improved lignin c

149 ntages of metabolite profiling approaches to metabolic engineering in terms of accelerating enzyme di

150 Our results extend the frontier of metabolic engineering in thermophilic hosts, have the po

151 e have demonstrated the feasibility of using metabolic engineering in transgenic plants (Camelina sat

152 addresses commonly encountered obstacles in metabolic engineering, including chromosomal integration

153 lite repression, and is a frequent target of metabolic engineering interventions.

154 brought the foundational systems approach of metabolic engineering into focus.

155 ory parts, fragmenting synthetic biology and metabolic engineering into host-specific domains.

156 Metabolic engineering is a powerful biotechnological too

157 Plant metabolic engineering is commonly used in the production

158 Plant and microbial metabolic engineering is commonly used in the production

159 A key computational problem in metabolic engineering is finding efficient metabolic rou

160 e increasing role of compartmentalization in metabolic engineering is highlighted.

161 Metabolic engineering is the science of rewiring the met

162 A common strategy of metabolic engineering is to increase the endogenous supp

163 applications in plant synthetic biology and metabolic engineering is understanding the structural de

164 work gap filling, (ii) (13)C analysis, (iii) metabolic engineering, (iv) omics-guided analysis and (v

165 es have only recently been extended to plant metabolic engineering, mainly due to greater pathway com

166 a and yeasts were established in a classical metabolic engineering manner over several decades.

167 Heterologous pathways used in metabolic engineering may produce intermediates toxic to

168 has important applications in fields such as metabolic engineering, metabolic network analysis and me

169 s integration of metabolism and development, metabolic engineering, microbial activity and drug resis

170 ain optimization called multivariate modular metabolic engineering (MMME).

171 ctical applications in five main categories: metabolic engineering, model-directed discovery, interpr

172 mplexes could help inform new approaches for metabolic engineering, nanotechnology, and drug delivery

173 n commercially cultivated for over 50 years, metabolic engineering now seems necessary in order to ac

174 accharides in a highly homogenous manner via metabolic engineering of a promiscuous sugar nucleotide

175 nformation on a plant FBP appears useful for metabolic engineering of a wide range of crops to enhanc

176 ting the general utility of such factors for metabolic engineering of anthocyanins and anthocyanin-de

177 els of omega-7 FA accumulation by systematic metabolic engineering of Arabidopsis (Arabidopsis thalia

178 ith different tissues are fundamental to the metabolic engineering of artemisinin.

179 This review highlights recent advances in metabolic engineering of biofuel-synthesis pathways in E

180 ur results provide promising new targets for metabolic engineering of C5-yeasts and point to iron as

181 One critical step of this new strategy is metabolic engineering of cancer, namely, to induce expre

182 y expands the suite of enzymes available for metabolic engineering of carotenoid biosynthetic pathway

183 he regulatory control of carotenogenesis and metabolic engineering of carotenoids in light of plastid

184 An effective strategy was developed for the metabolic engineering of cell-surface GPIs and GPI-ancho

185 onal enzyme, AbCAS is a promising target for metabolic engineering of cis-abienol production.

186 These results suggest strategies for metabolic engineering of crop species for drought tolera

187 ivery of artemisinin and other drugs through metabolic engineering of edible plants.

188 ing fed-batch feeding strategies with direct metabolic engineering of host cells.

189 Metabolic engineering of industrial Saccharomyces cerevi

190 mining prenyl transferase specificity and in metabolic engineering of isoprenoid pathways, especially

191 Recent efforts have focused on metabolic engineering of lactic acid bacteria as they pr

192 lipid-recycling scheme opens new avenues for metabolic engineering of lipid composition in algae.

193 re emerging as promising alternatives to the metabolic engineering of living cells.

194 ave proven themselves to be powerful aids to metabolic engineering of microbes by providing quantitat

195 oded FRET-based nanosensor for methionine as metabolic engineering of microbial strains for the produ

196 Metabolic engineering of microorganisms for production o

197 Metabolic engineering of microorganisms such as Escheric

198 Metabolic engineering of microorganisms to produce desir

199 ant metabolism and opens the possibility for metabolic engineering of new compounds based on this sca

200 Recent progress in the metabolic engineering of nitrogen-containing plant natur

201 d in this species, indicating a strategy for metabolic engineering of novel antimicrobial compounds i

202 jQMM will facilitate the design and metabolic engineering of organisms for biofuels and othe

203 ctions using enzyme mixtures in vitro and of metabolic engineering of pathways in cells as factories

204 Metabolic engineering of plant carotenoids in food crops

205 light some of the challenges associated with metabolic engineering of plant natural products, includi

206 of MFA to increase the chances of success in metabolic engineering of plants is presented, recent pro

207 ion to direct extraction, recent progress in metabolic engineering of plants offers an alternative su

208 Metabolic engineering of polyketide synthase (PKS) pathw

209 review the current status and potential for metabolic engineering of polyketides in E. coli.

210 nd plant hosts provided proof of concept for metabolic engineering of pseudolaratriene.

211 Here we report the metabolic engineering of Saccharomyces cerevisiae to pro

212 Further implications for metabolic engineering of seed oil production are discuss

213 rcane and form the baseline for the rational metabolic engineering of sugarcane feedstock for bioener

214 ategy based on synthetic cancer vaccines and metabolic engineering of TACAs on tumor cells.

215 ctivation approach offers new strategies for metabolic engineering of terpenoid production.

216 fermentation products, like alcohols, during metabolic engineering of the bacterium.

217 Metabolic engineering of the carotenoid pathway in recen

218 Metabolic engineering of the oleaginous yeast Yarrowia l

219 ead attention and prompted research aimed at metabolic engineering of the pathway for isoprenoid over

220 Metabolic engineering of the volatile spectrum offers en

221 his work demonstrates the feasibility of the metabolic engineering of these insecticidal metabolites

222 Furthermore, metabolic engineering of this compound has been achieved

223 furnish a molecular target for breeding and metabolic engineering of this important crop plant.

224 ultivated for food use, as hosts for complex metabolic engineering of wax esters for lubricant applic

225 Metabolic engineering offers an alternative approach in

226 Metabolic engineering, on the contrary, aims to optimize

227 However, predictable metabolic engineering or breeding is limited by the inco

228 terest for applications in synthetic biology/metabolic engineering, our results describe a new type o

229 Great strides have been made in plant metabolic engineering over the last two decades, with no

230 an be widely applied in future chloroplastic metabolic engineering, particularly for crop plants.

231 Metabolic engineering presents a powerful strategy to im

232 We evaluated the algorithm on five example metabolic-engineering problems from the literature; for

233 A significant challenge to most metabolic engineering projects is the need for strong co

234 d be a valuable computational tool to assist metabolic engineering projects.

235 ve enabled a wide variety of applications in metabolic engineering, protein labeling, biomaterials co

236 To this end, metabolic engineering provides the technological platfor

237 a Delta12-desturase activity in our omega-3 metabolic engineering rationales for Camelina.

238 The metabolic engineering requires detailed knowledge of the

239 tudies have become an essential component of metabolic engineering research.

240 Synthetic biology and metabolic engineering seek to re-engineer microbes into

241 bolic pathways of sweet basil and developing metabolic engineering strategies for enhanced production

242 oupling of enhanced F6P synthesis with other metabolic engineering strategies for the production of m

243 Several metabolic engineering strategies have been explored to p

244 Metabolic engineering strategies to ferment "unnatural"

245 mportance of plant derived natural products, metabolic engineering strategies to yield unnatural prod

246 Metabolic engineering strategies work at three levels: i

247 g S. cerevisiae through rational and inverse metabolic engineering strategies, comprising the optimiz

248 scuss recent developments in knowledge-based metabolic engineering strategies, that is the gathering

249 ween elementary flux modes in glycolysis for metabolic engineering strategies.

250 critical insight for future phenylpropanoid metabolic engineering strategies.

251 ndition-specific investigations of in silico metabolic engineering strategies.

252 te network properties and develop and refine metabolic engineering strategies.

253 uppression may be a useful component of seed metabolic engineering strategies.

254 Here, we report a combinatorial metabolic engineering strategy based on an orthogonal tr

255 Here we describe a metabolic engineering strategy to inhibit the biosynthes

256 so established the feasibility of fatty acid metabolic engineering strategy undertaken to improve qua

257 or further modification, as have genomic and metabolic engineering studies in native xylose fermentin

258 nechocystis sp. PCC 6803 systems biology and metabolic engineering studies.

259 of redox enzymes plays an important role in metabolic engineering, synthetic biology, and biocatalys

260 ity of redox enzymes is an important tool in metabolic engineering, synthetic biology, and biocatalys

261 l complex phenotypes through applications of metabolic engineering, synthetic biology, and evolutiona

262 ized by heterologous expression in a modular metabolic engineering system in Escherichia coli Members

263 Here, a modular metabolic engineering system is used in a combinatorial

264 cerevisiae as a tool for synthetic biology, metabolic engineering, systems biology and genetic studi

265 te and malate at breaker stage to identify a metabolic engineering target that was subsequently teste

266 on the yeast surface by optimizing multiple metabolic engineering targets in a combinatorial manner.

267 er grass species that may serve as potential metabolic engineering targets.

268 We summarize here the current trends in metabolic engineering techniques and strategies for mani

269 ays, a significant improvement over existing metabolic engineering techniques.

270 to realize the full potential of new in vivo metabolic engineering technologies by bridging the gap b

271 In this review we discuss new approaches for metabolic engineering that have the potential to address

272 calculating metabolic fluxes, key targets in metabolic engineering, that incorporates data from 13C l

273 is a promising approach to a core problem of metabolic engineering-that of identifying genetic manipu

274 imilar to approaches established long ago by Metabolic Engineering, the two methods deviate significa

275 gital and analog logic, systems biology, and metabolic engineering, three areas of particular theoret

276 will discuss how new technologies can enable metabolic engineering to be scaled up to the industrial

277 This study provides a new paradigm in metabolic engineering to control and optimize metabolic

278 re efficient use of biomass hydrolysates and metabolic engineering to develop bioconversions using LA

279 Here we apply metabolic engineering to generate Escherichia coli that

280 Metabolic engineering to increase yields of biofuel-rele

281 It is a routine task in metabolic engineering to introduce multicomponent pathwa

282 can be used in applications that range from metabolic engineering to orthogonal control of transcrip

283 yrethrins and demonstrate the feasibility of metabolic engineering to produce components of these def

284 We conclude that metabolic engineering to produce high yields of novel se

285 an edible plant and opens the door to using metabolic engineering to systematically quantify the imp

286 Hence, BIS1 might be a metabolic engineering tool to produce sustainably high-v

287 With the advent of metabolic engineering tools, the successful reconstituti

288 rategies, folate biofortification of rice by metabolic engineering was successfully achieved a couple

289 To demonstrate biosensor utility in metabolic engineering, we apply the glucarate biosensor

290 proach that combined chemical synthesis with metabolic engineering, we generated a series of salinosp

291 n, via a combination of enzyme screening and metabolic engineering, we obtain a more than tenfold inc

292 cells have, however, been used for in vitro metabolic engineering, where coordinated biochemical pat

293 omass and present an innovative strategy for metabolic engineering whereby an undesirable redox state

294 ms, and thus propose new approaches of plant metabolic engineering, which are inspired by an ancient

295 izing rhizoremediation, protein engineering, metabolic engineering, whole-transcriptome profiling, an

296 We propose that next-generation plant metabolic engineering will improve current engineering s

297 In any future, metabolic engineering will soon rival and potentially ec

298 Metabolic engineering with phage genes followed by rando

299 is study can be potentially used for reverse metabolic engineering, with the objective to construct e

300 greatest potential environmental benefit of metabolic engineering would be the production of high-vo