ライフサイエンスコーパス: metabolic labeling

1 d secretion of VWF and this was confirmed by metabolic labeling.

2 ecipitation, cell surface biotinylation, and metabolic labeling.

3 mbardment MS, detailed NMR spectrometry, and metabolic labeling.

4 IR or sham radiation followed by brief (32)P metabolic labeling.

5 n ceruloplasmin were examined by pulse-chase metabolic labeling.

6 itro with purified proteins and in vivo with metabolic labeling.

7 n global protein synthesis, as determined by metabolic labeling.

8 corporated into yeast and mouse proteins via metabolic labeling.

9 on as verified by both mass spectrometry and metabolic labeling.

10 of Skp1, Skp1 was not destabilized based on metabolic labeling.

11 Because metabolic labeling allows internal control throughout sa

12 Metabolic labeling also indicated that a significant fra

13 Metabolic labeling analysis demonstrated that, to compen

14 We describe the combined use of 15N-metabolic labeling and a cysteine-reactive biotin affini

15 f glycosylated tissues in live mice by using metabolic labeling and a gadolinium-based bioorthogonal

16 on in primary CLL cells, measured using bulk metabolic labeling and a novel flow cytometry assay to q

17 understand the molecular pathogenesis using metabolic labeling and assays of proinsulin export and i

18 ntroduce unnatural functional groups through metabolic labeling and chemoenzymatic tagging; identific

19 Ac status of proteins using a combination of metabolic labeling and click chemistry-based mass taggin

20 PDGF) beta-type receptor, as demonstrated by metabolic labeling and co-immunoprecipitation.

21 In this study, we performed metabolic labeling and immunofluorescence staining of ne

22 Metabolic labeling and immunofluorescence studies of COS

23 Using a metabolic labeling and immunoprecipitation approach, we

24 ive for point mutations in the elastin gene, metabolic labeling and immunoprecipitation experiments w

25 Metabolic labeling and immunoprecipitation of ACE confir

26 Metabolic labeling and immunoprecipitation studies revea

27 Metabolic labeling and immunoprecipitation studies with

28 approximately 8 h in human) as determined by metabolic labeling and immunoprecipitation with anti-GCa

29 ive rates of leptin biosynthesis measured by metabolic labeling and immunoprecipitation.

30 Metabolic labeling and in vitro enzyme assays confirmed

31 Metabolic labeling and ligand binding analyses showed th

32 of glycosaminoglycan (GAG) were examined via metabolic labeling and liquid chromatography.

33 m the endoplasmic reticulum (ER) as shown by metabolic labeling and live-cell imaging.

34 Metabolic labeling and mass spectrometric analyses sugge

35 importance, this technique does not require metabolic labeling and may be used as a pharmacodynamic

36 o determining the structure of melanin using metabolic labeling and NMR spectroscopy.

37 escribe a genetic AND gate for cell-targeted metabolic labeling and proteomic analysis in complex cel

38 Metabolic labeling and pulse-chase experiments showed th

39 Metabolic labeling and reporter gene assays demonstrated

40 Protein spots were quantified by metabolic labeling and scintillation counting.

41 f newly synthesized ferritin was analyzed by metabolic labeling and SDS-PAGE electrophoresis.

42 Metabolic labeling and top-down mass spectrometry reveal

43 treatment were first identified using (15)N metabolic labeling and untargeted mass spectrometry with

44 Analysis by immunoblotting, metabolic labeling, and mass spectrometry demonstrated t

45 -dimensional gel electrophoresis techniques, metabolic labeling, and stable isotope labeling methods

46 ent methods, quantitative mass spectrometry, metabolic labeling, and Western blotting.

47 Here we describe a metabolic labeling approach based on incorporation of no

48 of ribose and base methylations, and a novel metabolic labeling approach is presented to allow identi

49 Metabolic labeling approaches identify the general prote

50 Transcription inhibition experiments and metabolic labeling assays argue that REF/Aly does not af

51 and protein expression by nuclear run-on and metabolic labeling assays showed a 5-12-fold enhancement

52 In contrast, metabolic labeling assays showed that SAHA decreased inc

53 Metabolic labeling assays showed that targeting these th

54 ER exit of ENaC was assayed after metabolic labeling by following the appearance of cleave

55 These results demonstrate that metabolic labeling can be used to provide additional con

56 Metabolic labeling, cell surface biotinylation, immobili

57 ned by enzyme-linked immunosorbent assay and metabolic labeling, COL1A1 steady-state mRNA levels, and

58 Our work demonstrates the power of metabolic labeling combined with stable isotopic dilutio

59 Pulse-chase metabolic labeling confirmed that inhibiting calpains in

60 Using metabolic labeling, confocal immunofluorescence microsco

61 Using metabolic labeling coupled with cell surface biotinylati

62 Metabolic labeling coupled with sulfoamino acid analysis

63 K2 in a dose- and time-dependent manner, and metabolic labeling demonstrated that geldanamycin rapidl

64 Metabolic labeling demonstrates that these cysteines in

65 Ingenious metabolic labeling enables facile imaging of glycostruct

66 A cell metabolic labeling experiment can be completed in approx

67 We carried out metabolic labeling experiments and studies of mice with

68 ids suggest caution in the interpretation of metabolic labeling experiments and the necessity for inc

69 However, analysis of data from metabolic labeling experiments can be complicated becaus

70 Metabolic labeling experiments confirmed a reduced half-

71 Furthermore, metabolic labeling experiments demonstrated carbon from

72 Metabolic labeling experiments demonstrated hypoxia-medi

73 Radiotracer and metabolic labeling experiments demonstrated specific cel

74 Metabolic labeling experiments demonstrated that cells c

75 RNA blot hybridization, immunostaining, and metabolic labeling experiments demonstrated that SVAS ce

76 Metabolic labeling experiments demonstrated that the pre

77 Metabolic labeling experiments in primary hepatocytes fr

78 However, metabolic labeling experiments indicate that Syk is indu

79 be quite complex, in particular with in vivo metabolic labeling experiments producing fractional atom

80 Metabolic labeling experiments provided direct evidence

81 Metabolic labeling experiments revealed that Cia protein

82 Metabolic labeling experiments revealed that IFN had lit

83 Moreover, well-controlled cotransfection and metabolic labeling experiments revealed that VHL missens

84 Confocal microscopy and metabolic labeling experiments show that the total pool

85 Metabolic labeling experiments showed that class I is re

86 these more stringent regulatory properties, metabolic labeling experiments showed that coenzyme A (C

87 Finally metabolic labeling experiments showed that the cardiac r

88 Metabolic labeling experiments showed that the proteasom

89 This is in agreement with in vivo metabolic labeling experiments showing that fucose is no

90 lity was further examined in immunoblots and metabolic labeling experiments using two time points.

91 Through metabolic labeling experiments we demonstrate that the r

92 In vivo and in vitro metabolic labeling experiments were used to examine the

93 Furthermore, metabolic labeling experiments with (13)C-glucose showed

94 acylation of expressed HA as demonstrated by metabolic labeling experiments with [(3)H]palmitate.

95 Metabolic labeling experiments with [(35)S]methionine in

96 35S-Metabolic labeling experiments with domain-specific surf

97 Metabolic labeling experiments with transgenic parasites

98 Envelope is useful for planning or designing metabolic labeling experiments, by visualizing hypotheti

99 By reverse genetics and metabolic labeling experiments, we demonstrate that two

100 e rates of HDV RNA synthesis, as measured by metabolic labeling experiments, were identical at 4 and

101 DOC secreted the Cia proteins, as judged by metabolic labeling experiments.

102 hages synthesize P-selectin, as indicated by metabolic labeling experiments.

103 Metabolic labeling followed by immunoprecipitation revea

104 Metabolic labeling followed by immunoprecipitation verif

105 icular proteins by in vivo [(35)S]methionine metabolic labeling followed by preparation of highly pur

106 y cAMP or taurocholate and [(35)S]methionine metabolic labeling followed by subcellular fractionation

107 Proteins were also visualized using metabolic labeling followed by two-dimensional electroph

108 Characterization of these peptides by metabolic labeling, immunoprecipitation with Abeta-speci

109 munofluorescence microscopy, immunoblotting, metabolic labeling, immunoprecipitation, and carbohydrat

110 Metabolic labeling, immunoprecipitation, and SDS-polyacr

111 K44A, as detected by three distinct methods: metabolic labeling, immunoprecipitation/Western blotting

112 We now find, by metabolic labeling-immunoprecipitation experiments, that

113 Metabolic labeling in combination with mutagenesis indic

114 Current work utilized metabolic labeling in cultured cells expressing wild typ

115 Metabolic labeling in vivo demonstrated that E7 proteins

116 many of the possible issues associated with metabolic labeling, including low incorporation of sugar

117 Metabolic labeling indicated that the synthesis of these

118 se-chase experiments using [(35)S]methionine metabolic labeling indicated that the turnover rate of d

119 Metabolic labeling, ion exchange and size exclusion chro

120 many available isotopic labeling strategies, metabolic labeling is attractive for the excellent inter

121 Recently, we reported a novel metabolic labeling method to introduce the diazirine pho

122 ine these technologies with (13)C- and (15)N-metabolic labeling, multiple derivatization and ionizati

123 Following metabolic labeling, mutant CD-MPRs were tested for their

124 Metabolic labeling of a mutant PC12 cell line, A123.7, e

125 However, metabolic labeling of A34 transgenic mice with (75)Se re

126 can also be used to determine intracellular metabolic labeling of amino acids from glucose carbons.

127 In this study, metabolic labeling of bacteria with fatty acid chemical

128 seria meningitidis serogroup B to facilitate metabolic labeling of bacterial endotoxin and compared i

129 c cell membrane was used in conjunction with metabolic labeling of bacterial proteins to identify chl

130 bundance and half-life were determined after metabolic labeling of CCS-/- fibroblasts transfected wit

131 Metabolic labeling of cells expressing the siRNA to GRWD

132 sly identify the relevant peptide from TSR1, metabolic labeling of cells expressing TSR1 and the cyst

133 Metabolic labeling of cells using heavy amino acids is m

134 Reciprocal (14)N/(15)N metabolic labeling of cells was used to measure the rela

135 The results presented here indicate that metabolic labeling of cells with [(35)S]methionine under

136 Kinetic metabolic labeling of cells with [3H]-leucine indicated

137 The metabolic labeling of cells with an alkynyl derivative o

138 Metabolic labeling of cells with low-energy beta-emittin

139 Metabolic labeling of cellular proteins with [35S]methio

140 s examined by immunoblot analysis and (64)Cu metabolic labeling of Chinese hamster ovary cells transf

141 Metabolic labeling of CHO-K1 cells or Lec35.1 cells demo

142 to the biological system of interest (i.e., metabolic labeling of clinical samples, most animals, or

143 through in vivo [(14)C]acetate and [(3)H]2O metabolic labeling of developing seeds surprisingly reve

144 Metabolic labeling of DGA1 deletion strains with triglyc

145 is and turnover were examined following 64Cu metabolic labeling of fibroblasts derived from CCS+/+ an

146 ar abnormal Tf IEF patterns were analyzed by metabolic labeling of fibroblasts with inverted question

147 Metabolic labeling of glycans with a bioorthogonal chemi

148 Metabolic labeling of glycans with synthetic sugar analo

149 Metabolic labeling of hCTPS2 with [(32)P]H(3)PO(4) demon

150 on, fluorescence-activated cell sorting, and metabolic labeling of HCV-specific proteins.

151 Metabolic labeling of hexaacylated endotoxin (LOS) from

152 Metabolic labeling of hippocampal slices shows increased

153 ferential [(14)C]acetate and [(14)C]malonate metabolic labeling of hydroxylase-expressing seeds indic

154 Metabolic labeling of live animals with bromodeoxyuridin

155 Steady-state metabolic labeling of loa1Delta revealed a significant r

156 Metabolic labeling of M. bovis BCG showed that at least

157 rial activity of macrophages was assessed by metabolic labeling of M. tuberculosis with [3H]uracil.

158 ficiencies were quantitated by intracellular metabolic labeling of monocistronic mRNAs and the dicist

159 In addition, metabolic labeling of MR cells with (75)Se revealed a lo

160 lation of smaller molecules was supported by metabolic labeling of mtDNA with [3H]thymidine during re

161 full-length cytochrome b was undetectable by metabolic labeling of mutant cells, and these cells were

162 Both metabolic labeling of nascent transcripts and an unbiase

163 e analysis of transcriptional initiation via metabolic labeling of nascent transcripts in patient-der

164 In this method, metabolic labeling of newly synthesized proteins with AH

165 e analysis of transcriptional initiation via metabolic labeling of newly synthesized transcripts in l

166 The approach was validated by metabolic labeling of nuclear pore protein p62, which is

167 thod utilizes an O-GlcNAc azide analogue for metabolic labeling of O-GlcNAc-modified proteins, which

168 Metabolic labeling of one organism containing an orphan

169 rate of PPARgamma protein but decreased the metabolic labeling of PPARgamma protein using [(35)S]met

170 Metabolic labeling of primary hepatocytes indicated that

171 Using metabolic labeling of primary islets and a cultured beta

172 Cell-selective metabolic labeling of proteins with noncanonical amino a

173 The long-term metabolic labeling of rats with a diet enriched in 15N d

174 Second, metabolic labeling of Rce1-deficient cells revealed that

175 Metabolic labeling of recombinant interferon-beta and Gl

176 Here we combine metabolic labeling of RNA at high temporal resolution wi

177 we developed a chemical-genetic strategy for metabolic labeling of RNA.

178 hich contains an alkyne and a diazirine, for metabolic labeling of S-palmitoylated proteins and photo

179 Metabolic labeling of S. aureus confirms that CoA levels

180 Metabolic labeling of sialic acids in fibroblasts confir

181 ion was found on serum proteins, and reduced metabolic labeling of sialic acids was found in fibrobla

182 Recent development in metabolic labeling of small biomolecules allows the stud

183 We have used metabolic labeling of staphylococcal cultures with [(32)

184 In contrast, metabolic labeling of tectal slices in vitro documented

185 Metabolic labeling of the fibroblast cultures was used t

186 In IsoTaG, metabolic labeling of the glycoproteome is combined with

187 When the pool is filled with nascent ATP, metabolic labeling of the Na(+)/K(+) or Ca(2+) pump phos

188 Metabolic labeling of the protein in vivo indicates that

189 However, selective metabolic labeling of the target tissues in vivo remains

190 Metabolic labeling of THP-1 cells did not show release o

191 pectrometry with clinically accepted in vivo metabolic labeling of tissue with deuterium to generate

192 Metabolic labeling of transfected Chinese hamster ovary

193 geranylgeranylation of Rab24, determined by metabolic labeling or detergent partitioning assays, is

194 Following metabolic labeling or SEEL, tagged glycoproteins were en

195 Metabolic labeling or separation by isoelectric focusing

196 essment of purities of labeled compounds and metabolic labeling patterns requires careful analysis of

197 Using metabolic labeling, phosphoamino acid analysis, and muta

198 Using a [32P]orthophosphate metabolic labeling procedure to study HDV RNA replicatio

199 mical labeling quantification in addition to metabolic labeling quantification.

200 Metabolic labeling reaffirmed that metformin promoted wi

201 Indeed, metabolic labeling revealed an increased usage of ethano

202 Immunoblot analysis and pulse-chase metabolic labeling revealed that hephaestin is synthesiz

203 Metabolic labeling revealed that only wild type galectin

204 Metabolic labeling reveals a notable deficit in the rate

205 icing rates genome-wide in Drosophila, using metabolic labeling/RNA sequencing and new mathematical m

206 Here, we combine RNA metabolic labeling, rRNA-depleted RNA-seq, and DRiLL, a

207 Metabolic labeling showed incorporation of [(3)H]myristi

208 The results of in vivo metabolic labeling showed that Rosi markedly reduced de

209 nnosylation sites), using mass spectrometry, metabolic labeling, site-directed mutagenesis, and expre

210 oration in cell or tissue culture ((1)N/(1)N metabolic labeling, stable isotope labeling by amino aci

211 evaluate postgrowth Cys-labeling and 14N/15N metabolic labeling strategies for determination of relat

212 litated using chemical tags such as ICAT and metabolic labeling strategies with stable isotopes.

213 tude of dynamic range which is comparable to metabolic labeling strategies.

214 ecently developed neutron encoding (NeuCode) metabolic labeling strategy and parallel reaction monito

215 We have used a bioorthogonal metabolic labeling strategy to detect cell surface glyca

216 Here, we use a metabolic labeling strategy to directly measure nucleoso

217 In the 14N/15N metabolic labeling strategy, we achieve 98% 15N incorpor

218 Metabolic labeling studies and phosphopeptide mapping re

219 Using metabolic labeling studies as well as site-directed muta

220 Pulse-chase metabolic labeling studies demonstrate that acquisition

221 Metabolic labeling studies demonstrate that unlike wild

222 Furthermore, (32)P metabolic labeling studies demonstrated that MEK(SP) cle

223 Pulse-chase metabolic labeling studies demonstrated that the SPP hom

224 Using steady-state kinetics and in vivo metabolic labeling studies in modified yeast strains, we

225 Metabolic labeling studies in vivo demonstrate agonist-s

226 Metabolic labeling studies indicate that TfR2 protein le

227 Metabolic labeling studies indicated that this reflected

228 Metabolic labeling studies of Fibrillin-1 in human SSc d

229 Metabolic labeling studies of T. cruzi suggested that st

230 incorporation of [3H]palmitate into RGS16 in metabolic labeling studies of transfected cells or into

231 Metabolic labeling studies reveal that a synaptically ta

232 Moreover, results of metabolic labeling studies showed that downregulation of

233 Metabolic labeling studies showed that overexpression of

234 These findings were further supported by metabolic labeling studies that showed [1-(14)C]acetate

235 Metabolic labeling studies using [14C]20:4,n6 (at 100 mi

236 Metabolic labeling studies were conducted in freshly iso

237 Metabolic labeling studies with [(13)C]glucose showed th

238 sing phospho-specific Western blot analysis, metabolic labeling studies, and whole-cell signaling exp

239 Here we show, with metabolic labeling studies, that lopinavir leads to the

240 This progerin was farnesylated, as judged by metabolic labeling studies.

241 arbonitrile (PCN)-treated rats using in vivo metabolic-labeling studies with [35S]cysteine/methionine

242 Further, metabolic labeling suggests that silencing occurs before

243 Here, we use a recently developed metabolic labeling technique, NeuCode (neutron encoding)

244 ly confirmed using a 35S-methionine/cysteine metabolic labeling technique, whereas APP mRNA level rem

245 ation of cellular RNAs with polysomes and by metabolic labeling, that PDK-1-/- embryonic stem (ES) ce

246 By using standards generated from in vitro metabolic labeling, the relative quantitation of four pe

247 al-abundance or (15)N-labeled algae, we used metabolic labeling to compare protein levels in colonic

248 by this hormone in vivo, we used (14)N/(15)N metabolic labeling to perform a quantitative untargeted

249 alance during activation of T cells, we used metabolic labeling to quantify the contributions of RNA

250 arval zebrafish or paired with cell-specific metabolic labeling to visualize circuits underlying memo

251 rotein-labeling methods, such as chemical or metabolic labeling, to realize the same benefits.

252 reased Gln uptake and ammonia secretion, and metabolic labeling using (13)C-Gln revealed that Hace1 l

253 Metabolic labeling using sugar analogs compatible with c

254 Phosphorylation of DAT assessed by (32)PO(4) metabolic labeling was increased up to 2-fold by in vitr

255 surface cross-linking, FRET, and pulse-chase metabolic labeling, we demonstrate that deleting the cyt

256 Using immunoprecipitation coupled with metabolic labeling, we demonstrate that the alpha4 subun

257 sition of endoglycosidase H resistance after metabolic labeling, we found no evidence of ER retention

258 of gene expression and in vivo and in vitro metabolic labeling, we found that TbPSS2 (i) is necessar

259 By immunofluorescence and pulse-chase metabolic labeling, we found that the soluble constructs

260 By metabolic labeling, we here identify phosphatidylinosito

261 By using metabolic labeling, we measured Abeta42 and Abeta40 prod

262 bimolecular fluorescence complementation and metabolic labeling, we show that GABA(B) receptors assoc

263 this study, using in situ hybridization and metabolic labeling, we show that the mRNAs encoding euka

264 he set of tools available for cell-selective metabolic labeling, we sought a MetRS variant capable of

265 This was demonstrated by metabolic labeling, Western blotting, flow cytometry, an

266 The SILIS was produced through metabolic labeling where (1)(5)N was uniformly introduce

267 ted Raman scattering microscopy coupled with metabolic labeling with (13)C-phenylalanine is used to v

268 Metabolic labeling with (32)P(i) indicated that the aero

269 In vivo phosphorylation was studied by metabolic labeling with (32)P-orthophosphate.

270 tent was determined by IRMA and synthesis by metabolic labeling with (35)S-cysteine in organ cultures

271 Metabolic labeling with (64)Cu demonstrated that the red

272 GU in Aeromonas salmonicida was confirmed by metabolic labeling with (75) Se or mass spectrometry.

273 This computational screen and subsequent metabolic labeling with (75)Se and characterization of s

274 selenium into new proteins was confirmed by metabolic labeling with (75)Se, and expression of SelT w

275 s lipid modified in vivo, as demonstrated by metabolic labeling with [(3)H]myristate and [(3)H]palmit

276 that TLT-1 is a palmitoylated protein using metabolic labeling with [(3)H]palmitate and identified t

277 Metabolic labeling with [(3)H]palmitate determined that

278 lls, and the H1 proteins were analyzed after metabolic labeling with [(3)H]palmitate.

279 and constitutively active MEK-1 followed by metabolic labeling with [(32)P]orthophosphate indicated

280 nine lens epithelial cells was determined by metabolic labeling with [(35)S]-methionine.

281 Metabolic labeling with [(35)S]cysteine was used to char

282 ation with membrane-impermeable reagents and metabolic labeling with [(35)S]methionine followed by im

283 Using metabolic labeling with [(35)S]methionine to measure sub

284 Using metabolic labeling with [(35)S]methionine, the half-life

285 ynthesis in these cultures was determined by metabolic labeling with [(35)S]methionine.

286 ucceeded in detecting HDV RNA replication by metabolic labeling with [32P]orthophosphate in vivo and

287 Metabolic labeling with [35S]-methionine demonstrated th

288 ractions, the endotoxins were purified after metabolic labeling with [3H]- or [14C]acetate.

289 Triton X-114 partitioning and metabolic labeling with [3H]palmitic acid suggested lipi

290 itative proteomics studies of yeast that use metabolic labeling with amino acids rely on auxotrophic

291 Glycans can be imaged by metabolic labeling with azidosugars followed by chemical

292 an emerging strategy, glycans are imaged by metabolic labeling with chemical reporters and subsequen

293 Metabolic labeling with cytochrome oxidase (CO) and elec

294 Metabolic labeling with deuterated (isopropyl-d(7))-L-le

295 Here we describe the use of metabolic labeling with deuterium ((2)H) from (2)H(2)O a

296 Metabolic labeling with GalNAz followed by Staudinger li

297 azidoacetylmannosamine (ManNAz), showed that metabolic labeling with GalNAz resulted in the greatest

298 Bax on two-dimensional gels and confirmed by metabolic labeling with inorganic [(32)P]phosphate in He

299 O-linked glycoproteins in living animals by metabolic labeling with N-azidoacetylgalactosamine (GalN

300 Metabolic labeling with S(35)-methionine was used to det