ライフサイエンスコーパス: metacyclic

1 at PL cells were haploid relative to diploid metacyclics.

2 n was associated with a high mean percent of metacyclics (66%-82%) rather than total GMPL (2.0 x 10(4

3 To study this, we measured the adhesion of metacyclic and logarithmic-phase L. major promastigotes

4 e phosphorylation specifically in infectious metacyclics and amastigotes and promoting parasite survi

5 e RNA binding protein 6 to obtain infectious metacyclics and determined their protein and mRNA repert

6 We showed that metacyclics are quiescent cells, and propose this influe

7 could readily differentiate into infectious metacyclic cells but these were unable to establish infe

8 During differentiation to infective metacyclics, d-arabinopyranose (d-Arap) caps the LPG sid

9 on (171 bp) is required for full activity in metacyclic-derived trypanosomes.

10 on very close to the initiation site used in metacyclic-derived trypanosomes.

11 Our results demonstrate that amastigotes and metacyclics efficiently enter and activate DCs of both g

12 and failed to differentiate to the infective metacyclic form.

13 setse, including the generation of infective metacyclic forms expressing the variant surface glycopro

14 tural transmission, we used sand fly-derived metacyclic forms of L. major and preexposed the injectio

15 The infectious metacyclic forms of Trypanosoma brucei result from a com

16 le concerning the gene expression profile of metacyclic forms.

17 eath occurred not by apoptosis or changes in metacyclic gene expression, but from catastrophic proble

18 Metacyclics have a largely bloodstream-form type transcr

19 sites develop from amastigotes to infectious metacyclics, highlighting recent findings concerning the

20 w that a mucin expressed and secreted by the metacyclic infective form of T. cruzi, AgC10, is able to

21 dermal forehead challenge infection with 107 metacyclic L. amazonensis promastigotes at 4 wk demonstr

22 the parasite inoculation site, we introduced metacyclic L. infantum chagasi promastigotes intradermal

23 Now we demonstrate that metacyclic L. major promastigotes are poor inducers of I

24 Metacyclic Leishmania infantum chagasi promastigotes wer

25 Of biological significance, metacyclic lipophosphoglycan lacks the glucose residues

26 Metacyclic lpg5A(-)/lpg5B(-) promastigotes showed strong

27 d the largest changes between procyclics and metacyclics, observed at both the transcript and protein

28 e spt2- failed to differentiate to infective metacyclic parasites and died instead.

29 to bind to sand fly midguts in vitro whereas metacyclic parasites and LPG lost this capacity.

30 te this differential effect of procyclic and metacyclic parasites in terms of IL-12 induction, both s

31 though the level of scAra caps is maximal in metacyclic parasites, scbetaAraT activity is maximal in

32 -mice were inoculated with 250, 500, and 750 metacyclic parasites.

33 ring this process that leads to an activated metacyclic primed for invasion.

34 , since blocking macrophage Mac-1 diminishes metacyclic promastigote adhesion to a greater extent tha

35 attaching to the gut wall, to a nondividing metacyclic promastigote stage that is unable to attach t

36 the sand fly vector to the highly infective metacyclic promastigote stage.

37 iding further support for the role of PGs in metacyclic promastigote virulence.

38 tion between myeloid-derived human DC and Lm metacyclic promastigotes (infectious-stage parasites) to

39 that: 1) they are expressed predominantly in metacyclic promastigotes (the form in the insect vector

40 tiation into the vertebrate infective forms, metacyclic promastigotes and amastigotes.

41 the parasite during differentiation both to metacyclic promastigotes and to amastigotes, autophagoso

42 f inoculation of infectious Leishmania major metacyclic promastigotes by sand flies.

43 e mice were challenged by inoculation of 100 metacyclic promastigotes in the ear dermis.

44 s leishmaniasis using 10(2) Leishmania major metacyclic promastigotes inoculated into the footpads of

45 wth of L. major following inoculation of 100 metacyclic promastigotes into the ear dermis.

46 L. amazonensis metacyclic promastigotes lacking one SODA allele failed

47 infected intradermally in the ear with 10(5) metacyclic promastigotes of L. amazonensis together with

48 The infectious metacyclic promastigotes of Leishmania protozoa establis

49 more abundantly expressed in amastigotes and metacyclic promastigotes than in procyclic promastigotes

50 e efficient adhesion of complement-opsonized metacyclic promastigotes to cells expressing both recept

51 the initial complement-dependent adhesion of metacyclic promastigotes to human monocyte-derived macro

52 igotes and, to a much lesser extent, that of metacyclic promastigotes to induce IL-12 were enhanced b

53 depends on the ability of insect-transmitted metacyclic promastigotes to invade mammalian hosts, diff

54 The stable adhesion of complement-opsonized metacyclic promastigotes to Mac-1 is a prerequisite for

55 1,000 metacyclic promastigotes were coinoculated with a saliva

56 tures of natural transmission: low dose (100 metacyclic promastigotes) and inoculation into a dermal

57 In contrast to the infective parasites (metacyclic promastigotes), the procyclic promastigotes c

58 old was also required for the development of metacyclic promastigotes, as SODA/DeltasodA cultures wer

59 ventional experimental model employing 10(6) metacyclic promastigotes, in which the rapid development

60 racellular Leishmania to mammalian-infective metacyclic promastigotes, perhaps orchestrating the clea

61 us leishmaniasis (Lm), once loaded with live metacyclic promastigotes, were found to reactivate autol

62 fective procyclic promastigotes to infective metacyclic promastigotes.

63 SP and MSP-like protein (MLP), from virulent metacyclic promastigotes.

64 lenged in the ear with live Leishmania major metacyclic promastigotes.

65 for the phagocytosis of complement-opsonized metacyclic promastigotes.

66 3' UTRs uncovered: (1) previously identified metacyclic-specific expressed genes; (2) cloned genes wh

67 ogarithmic phase of growth to the infectious metacyclic stage does not affect this interaction.

68 t activated when VSG synthesis begins in the metacyclic stage in the tsetse fly salivary glands, are

69 of Leishmania infantum chagasi to a virulent metacyclic stage, as did the expression of PGF2alpha syn

70 nd differentiation into the animal-infective metacyclic stage.

71 sidues upon differentiation to the infective metacyclic stage.

72 um before differentiating into the infective metacyclic stages.

73 hagosomes being particularly numerous during metacyclic to amastigote form transformation.

74 Metacyclic trypanosomes, which inhabit the tsetse saliva

75 ng to each gene in both the epimastigote and metacyclic trypomastigote developmental stages.

76 hosphorylase a by T. cruzi epimastigotes and metacyclic trypomastigote extracts.

77 tigotes undergo before they develop into the metacyclic trypomastigote stage.

78 differentiation from the epimastigote to the metacyclic trypomastigote stage.

79 Trypanosoma cruzi metacyclic trypomastigotes (MT), but not blood form tryp

80 deficient epimastigotes readily converted to metacyclic trypomastigotes and efficiently infected mamm

81 We found that T. cruzi metacyclic trypomastigotes induced microvesicle release

82 d to extracellular amastigote-like cells and metacyclic trypomastigotes more rapidly than wild-type p

83 native conjunctival challenges with T. cruzi metacyclic trypomastigotes using a combination of immuno

84 fically reacted with a 55-kDa TcGP63 form in metacyclic trypomastigotes, suggesting stage-specific ex

85 ulture-derived trypomastigotes but 55 kDa in metacyclic trypomastigotes.

86 astigotes but distributed intracellularly in metacyclic trypomastigotes.

87 acellular and lacking in N-linked glycans in metacyclic trypomastigotes.

88 Determination of 23 kb of sequence at the metacyclic variant antigen type 4 (MVAT) vsg expression

89 e-associated gene I (ESAG-I) family occur in metacyclic variant antigen type 4 bloodstream trypanosom

90 e of these ESAG-I mRNAs are derived from the metacyclic variant antigen type 4 variant surface glycop

91 Activation of the metacyclic variant antigen type 7 (MVAT7) variant surfac

92 Certain VSGs are preferentially expressed in metacyclic versus bloodstream stages as a result of diff

93 However, the metacyclic VSG (M-VSG) genes, a small subset activated w

94 f Trypanosoma brucei rhodesiense expresses a metacyclic vsg as a monocistronic RNA from a promoter lo

95 The telomere-linked donor VSG 10.1 resembles metacyclic VSG expression sites, and is preceded by a cl

96 sion Site (BES)-linked VSGs and silencing of metacyclic VSGs (mVSGs) in BF cells are essential for an

97 ng metabolic enzymes exhibited expression in metacyclics with features of both procyclic and bloodstr