ライフサイエンスコーパス: metalloprotein

1 y fractions, 158 did not match any predicted metalloprotein.

2 n of a bound Mg(2+) ion reveals that FB is a metalloprotein.

3 L lactonase from Bacillus sp. 240B1 is not a metalloprotein.

4 ) x 10(11) M(-)(1), consistent with a Zn(2+) metalloprotein.

5 lly oxidize the amino acids bound to Cu in a metalloprotein.

6 on, suggesting that the target is not a zinc metalloprotein.

7 ally by harnessing its binding energy to the metalloprotein.

8 there has been no suggestion that TAG was a metalloprotein.

9 experiments show that Nkd is a zinc-binding metalloprotein.

10 ially incorporated into the active site of a metalloprotein.

11 viously shown that the E. coli TGT is a zinc metalloprotein.

12 e electronic structure and the function of a metalloprotein.

13 pplementation suggested the involvement of a metalloprotein.

14 various biological functions of O2-utilizing metalloproteins.

15 ovide metals for the periplasmic assembly of metalloproteins.

16 transition metals, typically in the form of metalloproteins.

17 ir occurrence as alpha-chiral amino acids in metalloproteins.

18 solvent-less (molten) liquids of functional metalloproteins.

19 ondria as a cofactor for several matrix zinc metalloproteins.

20 n to allow correct biochemical maturation of metalloproteins.

21 latter mechanism is especially possible with metalloproteins.

22 of how metalloproteins work is to design new metalloproteins.

23 ed investigations of paramagnetic centers of metalloproteins.

24 gated in a family of copper-containing redox metalloproteins.

25 conditions is performed for the suite of Fe-metalloproteins.

26 uantitative determination of iron-containing metalloproteins.

27 ation range of 0.1-100 microg/mL iron in the metalloproteins.

28 e(II)-, Co(II)-, and Ni(II)-binding sites of metalloproteins.

29 for studies of diamagnetic vanadium sites in metalloproteins.

30 d to determine the binding site of copper in metalloproteins.

31 ly one-third of all proteins estimated to be metalloproteins.

32 aryotic members of the PPP family, which are metalloproteins.

33 rameters that are applicable to redox-active metalloproteins.

34 r NO-induced release of Zn(2+) from cellular metalloproteins.

35 g the amino acid residues bound to copper in metalloproteins.

36 s an approach for simulating active sites of metalloproteins.

37 an be used to determine Fe-N-O geometries in metalloproteins.

38 S) to identify the binding site of copper in metalloproteins.

39 to mediate the transfer of hydrogen atoms in metalloproteins.

40 steine plays a key role as a metal ligand in metalloproteins.

41 ine-control of the structure and function of metalloproteins.

42 rtant properties of these prototypical redox metalloproteins.

43 reactions of NO with both model systems and metalloproteins.

44 on of possible evolutionary relationships of metalloproteins.

45 tal and has been widely used to characterize metalloproteins.

46 of these non-native peptides in the study of metalloproteins.

47 xperimentally accessing both redox states of metalloproteins.

48 he isozymes were shown to be zinc-containing metalloproteins.

49 g in many heme proteins, models, and related metalloproteins.

50 sical properties of the resulting artificial metalloproteins.

51 d highly structured ligands found in natural metalloproteins.

52 etry (MS) to identify Zn-bound histidines in metalloproteins.

53 f DNP in paramagnetically doped materials or metalloproteins.

54 f inhibitors that target clinically relevant metalloproteins.

55 ethod for identifying Zn-bound histidines in metalloproteins.

56 the Mn(II) ion in coordination complexes and metalloproteins.

57 centrations or in the maturation of secreted metalloproteins.

58 d that allows for robust characterization of metalloproteins.

59 is, metal tolerance, and the biosynthesis of metalloproteins.

60 l chelation, protein folding and function in metalloproteins, a family of de novo-designed peptides w

61 the vast majority, and the view is that most metalloproteins acquire their metals directly from cellu

62 Structure determination of the metalloprotein active site is obtained through a self-co

63 ic structure and coordination environment of metalloprotein active sites.

64 ifferent aspects of metal homeostasis and/or metalloprotein activity elicits distinct protective mech

65 nd Fe-S proteins, two other classes of redox metalloproteins, also possess ESE rate constants of appr

66 Both metalloprotein and flavin-linked sulfhydryl oxidases cat

67 e (NMR) spectroscopic shifts in paramagnetic metalloprotein and metalloporphyrin systems.

68 , these results indicate that NS5A is a zinc metalloprotein and that zinc coordination is likely requ

69 The discovery that Fur is a zinc metalloprotein and the use of surrogate metals for Fe(2+

70 All three enzymes are Zn2+ metalloproteins and also require Mg2+ for activity.

71 These highly covalent ligands are found in metalloproteins and are also used as models for Fe-O2 sy

72 ructures of transition-metal active sites in metalloproteins and chemical catalysts.

73 iniscent of the strategy followed by several metalloproteins and highlight the possible implication o

74 functional and bibliographic information on metalloproteins and other complex proteins, with an emph

75 e based on the radical processes mediated by metalloproteins and their synthetic analogs.

76 s that may be missing from studies of native metalloproteins and their variants, but also can result

77 n S = 0, (1)/(2), 1, (3)/(2), 2, and (5)/(2) metalloproteins and/or model systems.

78 her studies, it had been shown that PP1 is a metalloprotein, and in this study, we have largely focus

79 Our results indicate that Abeta in vivo is a metalloprotein, and the loosening of the structure follo

80 idea that the intracellular form of the PerR metalloprotein, and therefore its hydrogen peroxide sens

81 Eukaryotic cells contain hundreds of metalloproteins, and ensuring that each protein receives

82 the glass pores, react with the encapsulated metalloproteins, and establish the interprotein electron

83 complex, iron-containing redox cofactors of metalloproteins, and manage a myriad of biochemical tran

84 scent cluster, traffic the cluster to target metalloproteins, and regulate the assembly machinery in

85 To leverage the experimental metalloprotein annotations, we used a sequence-based de

86 The implication is that mononuclear metalloproteins are common targets of H(2)O(2) and that

87 ility has remained an enigma, because copper metalloproteins are prevalent and essential throughout a

88 The metalloproteins are reduced both chemically and electroc

89 active site analysis on the genome scale for metalloproteins as a class, revealing new insights into

90 c mobilization, implicating DNA-binding zinc metalloproteins as critical targets of NO-related antimi

91 The interstitial collagenase matrix metalloprotein-ase-1 (MMP-1) is up-regulated in the lung

92 How each metalloprotein assembles the correct metal at the proper

93 , in principle, be used to nucleate specific metalloprotein assemblies if introduced into proteins su

94 trix proteoglycans/glycoproteins is a Zn(2+) metalloprotein at physiological Zn(2+) concentrations.

95 Interactions of a model Cu-metalloprotein, azurin, with 10-100 nm silver nanopartic

96 le chemical transformations accessible using metalloprotein-based catalysts.

97 stead by cyanide ion until its toxicity with metalloproteins became a problem and primitive enzymes w

98 ve the reversible unfolding and refolding of metalloproteins because of a loss or decomposition of th

99 The DAHP synthase isozymes are metalloproteins, being activated in vitro by a variety o

100 ied Zn metalloproteins to validate predicted metalloprotein binding site structures.

101 is one of the most complicated processes in metalloprotein biochemistry.

102 rguably one of the most complex processes in metalloprotein biochemistry.

103 for enzyme-based bioelectrochemical sensors, metalloprotein bioelectronics, and energy research.

104 epresents a novel organism in which to study metalloprotein biology in that this spirochete has uniqu

105 pillary electrophoresis to follow a globular metalloprotein--bovine carbonic anhydrase II (BCA, EC 4.

106 This binding mode is rare among metalloproteins but well suited for an ultrasensitive ge

107 S-nitrosylation of Cu(II)-bound cysteine in metalloproteins, but also shed light on the reaction mec

108 ns that could be used for the measurement of metalloproteins by on-line IDMS analysis.

109 Azurin is a member of a family of metalloproteins called cupredoxins.

110 s, misallocation of the wrong metal ion to a metalloprotein can have resounding and often detrimental

111 Paramagnetic metalloproteins can be effective MRI sensors due to the

112 od before structure-function correlations of metalloproteins can be made on the basis of high-resolut

113 results show that structural and functional metalloproteins can be rationally designed in silico.

114 ause of their high affinity and selectivity, metalloproteins can be used as transducers in novel sens

115 The release of metal ions from metalloproteins can have significant biological conseque

116 prion protein (PrP) has been identified as a metalloprotein capable of binding multiple copper ions a

117 Metalloproteins catalyse some of the most complex and im

118 bene-mediated transformations accessible via metalloprotein catalysts and introduces a potentially ge

119 Ss, including resolved (1)H PCSs, in a large metalloprotein, Co(2+)-substituted superoxide dismutase

120 istent with annealing of an initially formed metalloprotein complex (k anneal = 0.4 min(-1)).

121 The kinetic stability of the metalloprotein complex can be traced to stabilization by

122 ion of modes, (2) optimization of the ligand-metalloprotein complex geometry by combined quantum mech

123 00 nm in diameter and is the largest natural metalloprotein complex known.

124 nding the assembly of this integral membrane metalloprotein complex.

125 A series of metalloprotein complexes embedded in a mitochondrial or

126 roperties, and interactions of the resulting metalloprotein complexes with azide, hydrogen peroxide,

127 2+) binding site within two de novo designed metalloprotein constructs using (111m)Cd perturbed angul

128 ts indicating that EutT was an oxygen-labile metalloprotein containing a redox-active metal.

129 Our structure reveals a new class of metalloprotein containing multinuclear iron clusters.

130 cate that (i) the Gram-positive pol III is a metalloprotein containing tightly bound zinc in a stoich

131 We also show that RsrA is a metalloprotein, containing near-stoichiometric amounts o

132 e was used as the plasmonic donor, while the metalloprotein cytochrome c was used as the acceptor mol

133 oteins have probed lambda and HAB in several metalloproteins (cytochrome c, myoglobin, azurin).

134 The Metalloprotein Database and Browser at The Scripps Resea

135 ut may have a catalytic mechanism similar to metalloprotein de-N-acetylases such as LpxC.

136 HDLP), using a modified scoring function for metalloproteins, demonstrate excellent agreement (R = 0.

137 atography separations of the iron-containing metalloproteins demonstrates the feasibility of the PB/H

138 he protein matrix upon the metal center make metalloprotein design a very fruitful area for the explo

139 Metalloprotein design and semiconductor nanoparticles ha

140 While metalloprotein design has now yielded a number of succes

141 A major goal in metalloprotein design is to build protein scaffolds from

142 This experiment is the first successful metalloprotein design that has a high coordination numbe

143 Metalloprotein design was used to generate a Pb(2+) ion

144 ed with regard to metalloprotein folding and metalloprotein design.

145 The metal binding preferences of most metalloproteins do not match their metal requirements.

146 n parallel with known metal substitutions of metalloproteins, driven by the Great Oxidation Event.

147 sibility of applications of this approach to metalloprotein drug targets, such as matrix metalloprote

148 in the four-helix bundle of de novo designed metalloprotein Duo Ferro 1.

149 is a good model for redox reactions between metalloproteins (electron carriers) and specific organic

150 ydrolase (PR-AMP cyclohydrolase) is a Zn(2+) metalloprotein encoded by the hisI gene.

151 ive metal required as a cofactor in multiple metalloproteins essential for a host of life processes.

152 Direct metal ligands to transition metals in metalloproteins exert a profound effect on protein-metal

153 Work on metalloprotein export in bacteria, and protein import in

154 er DNA with an affinity equal to that of the metalloprotein (Fe-SoxR), but lacks significant ability

155 ns and the redox activities of the resulting metalloprotein films.

156 itric oxide synthases (NOSs) are multidomain metalloproteins first identified in mammals as being res

157 The mechanism is discussed with regard to metalloprotein folding and metalloprotein design.

158 The metalloprotein follows the halogenation cycle, whereby c

159 environment, nature has evolved a panoply of metalloproteins for oxidative metabolism and protection

160 g a vector, we introduced an MRI reporter, a metalloprotein from the ferritin family, into specific h

161 rometry (ICP-MS) to characterize cytoplasmic metalloproteins from an exemplary microorganism (Pyrococ

162 To overcome this limitation, artificial metalloproteins have been created by incorporating compl

163 Detailed pathways for metal ion release from metalloproteins have been difficult to elucidate by clas

164 uring thermodynamic metal ion selectivity of metalloproteins have been performed, and the major deter

165 Consequently, metalloproteins have key roles in most biological proces

166 Many metalloproteins have the capacity to bind diverse metals

167 elationship between the activity of the zinc metalloprotein IDE and glucose homeostasis remains uncle

168 at protein phosphatase 1 may be an iron/zinc metalloprotein in vivo.

169 gate the structure-function relationships of metalloproteins in a minimal, well-defined and controlle

170 sensitive visualization and quantitation of metalloproteins in biological samples.

171 these biological assemblies by encapsulating metalloproteins in sol-gel silica glass and letting mobi

172 ssion protocols, sensitivity-enhanced NMR of metalloproteins in solution, the investigation of solven

173 owever, this technique cannot be extended to metalloproteins in solution.

174 actors represent the largest single class of metalloproteins in the human genome.

175 Little is known about how metalloproteins in the secretory pathway obtain their me

176 sfer Fe-S clusters to the appropriate target metalloproteins in vivo.

177 llowed to determine epsilon values for other metalloproteins in which metal binding contributes to th

178 ded the Rosetta design methodology to design metalloproteins in which the amino acid (2,2'-bipyridin-

179 The transferase is a Zn/Fe metalloprotein, in which the metal ions stabilize the st

180 ginosa azurin is a 128-residue beta-sandwich metalloprotein; in vitro kinetic experiments have shown

181 For some metalloproteins (including all transferrin family member

182 ty during cofactor assembly for a variety of metalloproteins, including adenosylcobalamin (AdoCbl)-de

183 e of the reduction potential E degrees for a metalloprotein indicates that the protonation state of a

184 tegy for the discovery of lead fragments for metalloprotein inhibition.

185 at despite their ability to bind metal ions, metalloprotein inhibitors are not prone to widespread of

186 e selected as well as several other reported metalloprotein inhibitors in order to represent a broad

187 Representative ligand-metalloprotein interaction energies are obtained by subs

188 Metal centers in metalloproteins involve multiple metal-ligand bonds.

189 tantial variations in concentrations of iron metalloproteins involved in nitrogen fixation and photos

190 y definition, the release of metal ions from metalloproteins involves the disruption of multiple meta

191 An important feature of the designed metalloprotein is its two cationic redox centers embedde

192 Cyt c, a heme containing metalloprotein is located in the intermembrane space of

193 Quality computational description of metalloproteins is a great challenge due to the vast spa

194 of semisynthetic copper(II)-based catalytic metalloproteins is described in which a metal-binding bi

195 nition of biologically active amino acids of metalloproteins is elicited by the presence of specific

196 analyze accurately and precisely individual metalloproteins is of increasing importance.

197 ting that species-specific ID-MS analysis of metalloproteins is possible.

198 nalysis of unpaired electron spin density in metalloproteins is presented, which allows a fast and ro

199 structural determination of active sites of metalloproteins is presented.

200 , a product of catabolism of heme-containing metalloproteins, is a key inducer of ROS.

201 parison to recent successes in designing non-metalloproteins, it is even more challenging to rational

202 the problems in the receptor-based design of metalloprotein ligands due to inadequacies in the force-

203 ctive intermediates and transition states in metalloproteins may be widespread in nature.

204 Other metalloproteins may have similarly adapted to using mang

205 In metalloproteins, metal centers serve as active sites for

206 series of 24 inorganic, organometallic, and metalloprotein/metalloporphyrin model systems in S = 0,

207 across a 16-mer helical bundle (three-helix metalloprotein) modified with a capping CoIII(bipyridine

208 Metalloproteins (MPs) comprise one-third of all known pr

209 allenging to design metalloproteins than non-metalloproteins, much progress has been made in this are

210 gn of a structural and functional model of a metalloprotein, nitric oxide reductase (NOR), by introdu

211 The two-component metalloprotein nitrogenase catalyzes the reductive fixat

212 We conclude that this bifunctional metalloprotein of the osteoclast is required for normal

213 als an organism assimilates and identify its metalloproteins on a genome-wide scale.

214 owever, existing MRI reporter genes based on metalloproteins or chemical exchange probes are limited

215 e show that the technique can be extended to metalloproteins or complex:protein interactions.

216 roles of Zn2+ donation to, or removal from, metalloproteins, or Zn2+ storage in vegetative plant tis

217 13C strongly hyperfine-shifted resonances in metalloprotein paramagnetic centers.

218 n microcrystalline powders of a paramagnetic metalloprotein permit NMR crystallography.

219 ESI-TOF mass spectra of the metalloproteins present in nondenaturing solutions exhib

220 f CusF (log K = 14.3 +/- 0.1), a periplasmic metalloprotein required for the detoxification of elevat

221 h Institute is a web-accessible resource for metalloprotein research.

222 ting from in situ chemical manipulation of a metalloprotein sample.

223 ectron paramagnetic resonance spectra of the metalloproteins show that encapsulation in sol-gel glass

224 -nitrosylation and denitrosylation involving metalloproteins, SNO lyase(s) and GSNO reductase.

225 nhance the understanding of similar sites in metalloproteins, specifically cobalt-substituted zinc en

226 butions of metal-protein interactions toward metalloprotein stability is largely due to an inability

227 ontribution of a Zn(II)-(S.Cys)4 site toward metalloprotein stability relevant to classic structural

228 Here we show that a de novo designed Zn(II) metalloprotein stabilizes a chemically reactive organic

229 n and evaluation of an isotopically enriched metalloprotein standard for use as a calibrant in specie

230 eins as a class, revealing new insights into metalloprotein structure and function.

231 etail is critical to a full understanding of metalloprotein structure--function relationships.

232 n provide a reliable balanced description of metalloproteins' structure, dynamics, and electronic str

233 binding site that appears to be unique among metalloproteins studied to date.

234 Among them, there are four metalloprotein subunits, including a 113 kDa iron-sulfur

235 ins two histidine motifs resembling those of metalloproteins such as fatty acid desaturases.

236 ficking required for the assembly of complex metalloproteins such as nitrogenase.

237 te (67)Zn NMR spectra of model compounds for metalloproteins, such as [H(2)B(3,5-Me(2)pz)(2)](2)Zn (p

238 em that affected to the levels of metals and metalloproteins, such as MT, Cu/Zn-SOD, or Mn-CA, the br

239 for understanding the mechanism of important metalloproteins, such as photosystem II.

240 lar procedures should be generally useful in metalloprotein systems.

241 though it is much more challenging to design metalloproteins than non-metalloproteins, much progress

242 oxygen ((1)O(2)) is mediated by ChrR, a zinc metalloprotein that binds to and inhibits the activity o

243 Transferrin (Tf) is an enigmatic metalloprotein that exhibits a profound conformational c

244 for the assembly of periplasmic and secreted metalloproteins that are essential for survival in extre

245 metal ions, with emphasis on copper(II), to metalloproteins that are hallmarks of these diseases - a

246 Transferrins constitute a class of metalloproteins that are involved in circulatory iron tr

247 Eukaryotic cells contain hundreds of metalloproteins that are supported by intracellular syst

248 es to date are the AHL lactonases, which are metalloproteins that belong to the metallo-beta-lactamas

249 [FeFe]-Hydrogenases are complex metalloproteins that catalyze the reversible reduction o

250 Superoxide dismutases (SODs) are metalloproteins that protect organisms from toxic reacti

251 s even more challenging to rationally design metalloproteins that reproduce both the structure and fu

252 we show that the FusB family are two-domain metalloproteins, the C-terminal domain of which contains

253 Nitrogenase consists of two component metalloproteins, the iron (Fe) protein and the molybdenu

254 built from interactions observed in simpler metalloproteins, they contain novel features that may be

255 For metalloproteins to acquire the right metals, metal senso

256 Biology employs three classes of metalloproteins to cover the majority of the 2-V range o

257 re important biological ligands that bind to metalloproteins to function crucially in processes such

258 Selective binding by metalloproteins to their cognate metal ions is essential

259 e structure data from two of the purified Zn metalloproteins to validate predicted metalloprotein bin

260 uctural characterization of de novo designed metalloproteins together with determination of chemical

261 Four iron-containing metalloproteins (transferrin, myoglobin, hemoglobin, and

262 n of cytochrome c (cyt c), a heme containing metalloprotein using its specific monoclonal antibody.

263 shifts in paramagnetic metalloporphyrins and metalloproteins using quantum chemical methods should op

264 uctural and dynamical determination of large metalloproteins using solid-state nuclear magnetic reson

265 That human BVR is a Zn metalloprotein was further substantiated by 65Zn exchang

266 A de novo designed coiled-coil metalloprotein was prepared that uses electrostatic inte

267 residues were coordinated with metals and 15 metalloproteins were endogenously modified supporting me

268 not equally active and due to the fact that metalloproteins were rarely used as drug targets.

269 the geometric structure and the dynamics of metalloproteins, when NMR parameters are available of nu

270 An extra layer of complexity is added in metalloproteins, where a metal cofactor participates in

271 es share properties with the active sites of metalloproteins, where function is correlated strongly w

272 Phage T4 gene 32 protein (gp32) is a zinc metalloprotein which binds cooperatively and preferentia

273 redoxins, rusticyanin is a copper-containing metalloprotein, which is composed of a core beta-sandwic

274 Pho8 appears to be a rare example of a metalloprotein whose stability is regulated by its metal

275 PerR is a dimeric, Zn2+-containing metalloprotein with a regulatory metal-binding site that

276 1 periplasmic, catalytic domain to be a zinc metalloprotein with an alkaline phosphatase/sulphatase f

277 characterization revealed a Bpy-Ala-mediated metalloprotein with the ability to bind divalent cations

278 after purification, indicating that Mca is a metalloprotein with zinc as the native metal.

279 cellular damage initiated by the reaction of metalloproteins with H2O2.

280 ethod also facilitates the de novo design of metalloproteins with novel structures and functions, inc

281 ng the rupture mechanism of metal centers in metalloproteins with unprecedented resolution by using s

282 purified STM1808 suggests that it is a zinc metalloprotein, with histidine residues H32 and H82 requ

283 detoxification, also deliver zinc to certain metalloproteins within intracellular compartments.

284 An ultimate test of our knowledge of how metalloproteins work is to design new metalloproteins.

285 essful design of a structural and functional metalloprotein would greatly advance the field of protei

286 nt with the hypothesis that E4 34k is a zinc metalloprotein, zinc binding by baculovirus-expressed E4