ライフサイエンスコーパス: methionyl-tRNA

1 ractions with the anticodon of the initiator methionyl tRNA.

2 n protein synthesis are aminoacylated to non-methionyl-tRNAs.

3 somes, mRNA's, tRNA's including an initiator methionyl-tRNA, aminoacyl tRNA synthetases, and other pr

4 plays a central role in binding of initiator methionyl-tRNA and mRNA to the 40 S ribosomal subunit to

5 sential role in the binding of the initiator methionyl-tRNA and mRNA to the 40S ribosomal subunit to

6 esponsible for extensive misacylation of non-methionyl tRNAs, and mismethionylation also occurs in th

7 MetRS readily misacylates non-methionyl tRNAs at frequencies of up to 10% in mammalian

8 tion of 80 S ribosomes, stabilizes initiator methionyl-tRNA binding to 40 S subunits, and is required

9 The specific formylation of initiator methionyl-tRNA by methionyl-tRNA formyltransferase (MTF)

10 The formylation of initiator methionyl-tRNA by methionyl-tRNA formyltransferase (MTF)

11 The specific formylation of initiator methionyl-tRNA by methionyl-tRNA formyltransferase (MTF)

12 Formylation of initiator methionyl-tRNA by methionyl-tRNA formyltransferase (MTF)

13 The specific formylation of initiator methionyl-tRNA by methionyl-tRNA formyltransferase (MTF;

14 It proposes that the 40S ribosome-methionyl-tRNA complex recognizes and binds to the 5'-en

15 uorescent probes were covalently attached to methionyl-tRNA(f) and tested for their incorporation int

16 he N-terminal domain is highly homologous to methionyl-tRNA(f)Met formyltransferase.

17 Met is misacylated to specific non-methionyl-tRNA families, and these Met-misacylated tRNAs

18 y believed to require a formylated initiator methionyl tRNA (fMet-tRNA(fMet)) for initiation.

19 oplasts normally uses a formylated initiator methionyl-tRNA (fMet-tRNA(f)(Met)).

20 ation complexes (ICs) that carry an N-formyl-methionyl-tRNA (fMet-tRNA(fMet)).

21 y believed to require a formylated initiator methionyl-tRNA (fMet-tRNAfMet) in a process involving in

22 shares 32% identity with Escherichia coli L-methionyl-tRNA formyltransferase (EC 2.1.2.9), was expre

23 hich the nuclear gene encoding mitochondrial methionyl-tRNA formyltransferase (FMT1) has been deleted

24 in an inducible manner the Escherichia coli methionyl-tRNA formyltransferase (MTF) in the cytoplasm

25 c formylation of initiator methionyl-tRNA by methionyl-tRNA formyltransferase (MTF) is important for

26 on of initiator methionyl-tRNA (Met-tRNA) by methionyl-tRNA formyltransferase (MTF) is important for

27 e formylation of initiator methionyl-tRNA by methionyl-tRNA formyltransferase (MTF) is important for

28 c formylation of initiator methionyl-tRNA by methionyl-tRNA formyltransferase (MTF) is important for

29 initiator methionyl-tRNA (Met-tRNA(Met)) by methionyl-tRNA formyltransferase (MTF) is important for

30 Formylation of initiator methionyl-tRNA by methionyl-tRNA formyltransferase (MTF) is important for

31 The formylation reaction is catalyzed by methionyl-tRNA formyltransferase (MTF) located in mitoch

32 c formylation of initiator methionyl-tRNA by methionyl-tRNA formyltransferase (MTF; EC 2.1.2.9) is im

33 sary for synthesis of 10-formyl-THF, and the methionyl-tRNA formyltransferase (open reading frame YBL

34 ed at the FMT1 locus, encoding mitochondrial methionyl-tRNA formyltransferase, lack detectable fMet-t

35 li overproducing aminoacyl-tRNA synthetases, methionyl-tRNA formyltransferase, or IF2, we identified

36 e same Rossmann fold as the related enzymes, methionyl-tRNA-formyltransferase and glycinamide ribonuc

37 10-formyltetrahydrofolate dehydrogenase and methionyl-tRNA-formyltransferase extends to the C termin

38 16-aa insertion loop present in eubacterial methionyl-tRNA formyltransferases (MTF) is critical for

39 ast 18S rRNA critical in vivo for recruiting methionyl tRNA(i)(Met) to 40S subunits during initiation

40 ong with the initiator transfer RNA N-formyl-methionyl-tRNA(i) (fMet-tRNA(i)(fMet)) and a short piece

41 In vitro, eIF-2 binds the initiator methionyl-tRNA in a GTP-dependent fashion.

42 sults indicate that formylation of initiator methionyl-tRNA is not required for mitochondrial protein

43 ment distinguishing initiator from elongator methionyl tRNA, is required for recognition of the methi

44 n occur without formylation of the initiator methionyl-tRNA (Met-tRNA(fMet)).

45 een shown to direct binding of the initiator methionyl-tRNA (Met-tRNA(i)) to 40 S ribosomal subunits

46 N-Formylation of initiator methionyl-tRNA (Met-tRNA(Met)) by methionyl-tRNA formylt

47 The specific formylation of initiator methionyl-tRNA (Met-tRNA) by methionyl-tRNA formyltransf

48 s region of the E. coli enzyme and initiator methionyl-tRNA (Met-tRNA) by using two complementary pro

49 ic initiation factor 2 (eIF2), the initiator methionyl-tRNA (Met-tRNA), and GTP is a critical step in

50 eIF2), GTP, and methionine-charged initiator methionyl-tRNA (met-tRNAi).

51 n synthesis, a ribosome with bound initiator methionyl-tRNA must be assembled at the start codon of a

52 These include SAH hydrolase, methionyl-tRNA synthase, 5-methyltetrahydrofolate:Hcy me

53 ments of the anticodon for aminoacylation by methionyl tRNA synthetase and IleRS.

54 ective at creating negative determinants for methionyl tRNA synthetase and positive determinants for

55 Here we describe a mutant murine methionyl-tRNA synthetase (designated L274GMmMetRS) that

56 both of which are activated by an engineered methionyl-tRNA synthetase (designated NLL-MetRS), are ex

57 Human mitochondrial methionyl-tRNA synthetase (human mtMetRS) has been ident

58 e-recombinase-induced expression of a mutant methionyl-tRNA synthetase (L274G) enables the cell-type-

59 In one case, the C-terminal disruption of methionyl-tRNA synthetase (MetG) results in a 10,000-fol

60 a strain carrying a single genomic copy of a methionyl-tRNA synthetase (MetRS) gene, metG*, engineere

61 x with glutamyl-tRNA synthetase (GluRSc) and methionyl-tRNA synthetase (MetRS) in the cytoplasm to re

62 ighly accurate, recent results show that the methionyl-tRNA synthetase (MetRS) is an exception.

63 aturation mutagenesis library of the E. coli methionyl-tRNA synthetase (MetRS) led to the discovery o

64 chains was used to identify a diverse set of methionyl-tRNA synthetase (MetRS) mutants that allow eff

65 The active site of methionyl-tRNA synthetase (MetRS) possesses two function

66 he centerpiece of the AND gate is a bisected methionyl-tRNA synthetase (MetRS) that charges the Met s

67 cylation of tRNA(Leu) with methionine by the methionyl-tRNA synthetase (MetRS).

68 anaerobiosis and antibiotic exposure via the methionyl-tRNA synthetase (MetRS).

69 In the work described here, human methionyl-tRNA synthetase (MRS) and human lysyl-tRNA syn

70 dified to lysidine to prevent recognition by methionyl-tRNA synthetase (MRS) and production of a chim

71 Previously, we demonstrated that the class I methionyl-tRNA synthetase aminoacylates RNA microhelices

72 itroso-Hcy is in fact transferred to tRNA by methionyl-tRNA synthetase and incorporated into protein

73 plication of the carboxyl-terminal domain of methionyl-tRNA synthetase and may direct tRNA to the act

74 Model enzymes, such as methionyl-tRNA synthetase and trypsin, were inactivated

75 ere we describe a rationally designed mutant methionyl-tRNA synthetase containing two point substitut

76 istakenly selected in place of methionine by methionyl-tRNA synthetase during protein biosynthesis, w

77 NA microarrays and filter retention that the methionyl-tRNA synthetase enzyme from Escherichia coli (

78 surrogate that requires a mutant form of the methionyl-tRNA synthetase for activation.

79 identical to the carboxyl-terminal domain of methionyl-tRNA synthetase from Caenorhabditis elegans, a

80 ort that heterologous expression of a mutant methionyl-tRNA synthetase from Escherichia coli permits

81 thioester of homocysteine, is synthesized by methionyl-tRNA synthetase in all cell types.

82 Urea-based methionyl-tRNA synthetase inhibitors were designed, synt

83 ogate, azidohomoalanine, is activated by the methionyl-tRNA synthetase of Escherichia coli and replac

84 Homocysteine (Hcy) editing by methionyl-tRNA synthetase results in the formation of Hc

85 ve identified mutations in the mitochondrial methionyl-tRNA synthetase, Aats-met, the homologue of hu

86 Mes1p, methionyl-tRNA synthetase, also suppresses the defect in

87 n is our finding that the plant Oryza sativa methionyl-tRNA synthetase, expressed in Escherichia coli

88 siological buffer conditions with wheat germ methionyl-tRNA synthetase, required mutation of the anti

89 em alone can be aminoacylated by the class I methionyl-tRNA synthetase.

90 cylated with methionine by overproduction of methionyl-tRNA synthetase.

91 tone in L. luteus, suggesting involvement of methionyl-tRNA synthetase.

92 ine concentration, suggesting involvement of methionyl-tRNA synthetase.

93 f the anticodon in aminoacylation of tRNA by methionyl-tRNA synthetase.

94 a similar base-pair uncoupling when bound to methionyl-tRNA synthetase.

95 Escherichia coli isoleucyl- and methionyl-tRNA synthetases are closely related enzymes t

96 (eIF2) bound to GTP transfers the initiator methionyl tRNA to the 40S ribosomal subunit.

97 Binding of initiator methionyl-tRNA to ribosomes is catalyzed in prokaryotes

98 1- and eIF1A-dependent delivery of initiator methionyl-tRNA to the 40 S ribosomal subunit and subsequ

99 e dissociation, the binding of the initiator methionyl-tRNA to the 40 S ribosomal subunit, and mRNA r

100 d through modulation of binding of initiator methionyl-tRNA to the 40 S ribosomal subunit.

101 on initiation factor eIF2 delivers initiator methionyl-tRNA to the 40 S ribosomal subunit.

102 Events regulating the binding of initiator methionyl-tRNA to the 40S ribosomal subunit were assesse

103 erhaps to direct or stabilize the binding of methionyl-tRNA to the ribosomal P site.

104 facilitating the proper binding of initiator methionyl-tRNA to the ribosomal P site.

105 2B controls the recruitment of the initiator methionyl-tRNA to the ribosome and is activated by insul

106 nucleotide exchange to deliver the initiator methionyl-tRNA to the ribosome.

107 overproduction of lysyl-tRNA synthetase and methionyl-tRNA transformylase results in partial formyla

108 ectly to supporting or stabilizing initiator methionyl tRNA (tRNA-Met(i)) association with the riboso

109 in its GTP-bound state to deliver initiator methionyl-tRNA (tRNA(i)(Met)) to the small ribosomal sub

110 nary complex (TC) with GTP and the initiator methionyl-tRNA (tRNAi), mediating ribosomal recruitment

111 gh-copy-number IMT genes, encoding initiator methionyl tRNA (tRNAiMet), or LHP1, encoding the yeast h

112 strate that Escherichia coli misacylates non-methionyl-tRNAs with methionine in response to anaerobio