ライフサイエンスコーパス: methylumbelliferyl

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1 4-Methylumbelliferyl-6-sulfo-2-acetamido-2-deoxy derivativ

2 measured using the fluorometric substrate 4-methylumbelliferyl-6-thiopalmitoyl-beta-D-glucoside.

3 ompared against alpha-naphthyl acetate and 4-methylumbelliferyl acetate for their ability to detect h

4 tant enzyme was shown to rapidly hydrolyse 4-methylumbelliferyl acetate in paraoxon-treated cells, wh

5 r catalytic efficiencies for hydrolysis of 4-methylumbelliferyl acetate, heroin, and 6-monoacetylmorp

6 group of cocaine and the acetyl groups of 4-methylumbelliferyl acetate, heroin, and 6-monoacetylmorp

7 nd two of these, alpha-napthyl acetate and 4-methylumbelliferyl acetate, identified an esterase of ap

8 he yellow p-nitrophenolate and fluorescent 4-methylumbelliferyl aglycons, respectively.

9 We tested the hypothesis that 4-methylumbelliferyl alpha-d-glucopyranoside (4MalphaG), a

10 activity for a fluorogenic substrate, 2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid (4-M

11 ed with a fluorometric assay using the 2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid (MUN

12 uorometric enzyme assay (FA) 1 (FA-1), 2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid (MUN

13 yranoside 6-phosphate (pNP alphaGlc6P) and 4-methylumbelliferyl-alpha-D-glucopyranoside 6-phosphate (

14 yl-alpha-D-glucopyranoside 6-phosphate and 4-methylumbelliferyl-alpha-D-glucopyranoside 6-phosphate w

15 96-kD protein, but it was not active with 4-methylumbelliferyl-alpha-D-glucopyranoside as the substr

16 n with the existing fluorogenic substrate, 4-methylumbelliferyl-alpha-D-glucopyranoside.

17 e mature form, but a lower Vmax and Km for 4-methylumbelliferyl-alpha-D-glucoside.

18 The rate of hydrolysis of 4-methylumbelliferyl-alpha-KDN (MU-KDN) by KDN-sialidase i

19 Oase, we have carried out the synthesis of 4-methylumbelliferyl-alpha-KDO (alpha-KDO-MU) by conjugati

20 In repeated studies, leukocyte 4 methylumbelliferyl-alpha-L-iduronidase activities in thi

21 against fluorogenic substrates, chitobiose-4-methylumbelliferyl and chitotriose-4-methylumbelliferyl,

22 biose-4-methylumbelliferyl and chitotriose-4-methylumbelliferyl, and enhances activity against chitoo

23 This substrate, 6, 8-difluoro-4-methylumbelliferyl beta-d-galactopyranoside (DiFMUG), wa

24 sensitive than the commonly used substrate 4-methylumbelliferyl beta-d-galactopyranoside (MUG), for t

25 novel fluorogenic substrate, 6,8-difluoro-4-methylumbelliferyl beta-D-xylobioside (DiFMUX(2)).

26 It rapidly cleaved p-nitrophenyl and 4-methylumbelliferyl beta-GlcNAc, was slightly active with

27 gue of the fluorescent cellulase substrate 4-methylumbelliferyl-beta-D-cellobioside (MU-CB).

28 opyranoside-6-phosphate (pNPbetaGlc6P) and 4-methylumbelliferyl-beta-D-glucopyranoside-6-phosphate (4

29 pyrrolidonyl-beta-naphthylamide (PYR), and 4-methylumbelliferyl-beta-D-glucuronide (MUG) was evaluate

30 -N,N'-diacetylchitobioside (MUF-diNAG) and 4-methylumbelliferyl-beta-D-N,N',N"-triacetylchitotrioside

31 drolyse the artificial lysozyme substrate, 4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotriosid

32 tern of activity toward the chitin analogs 4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside (MUF

33 to alanine or when cells were treated with 4-methylumbelliferyl-beta-d-xylopyranoside or chlorate to

34 face proteoglycans by a 72-h incubation in 4-methylumbelliferyl-beta-D-xyloside did not attenuate the

35 with inhibitors of proteoglycan synthesis (4-methylumbelliferyl-beta-D-xyloside) or sulfation (sodium

36 presence of a stimulator of CS synthesis, 4-methylumbelliferyl-beta-D-xyloside, reduced the amount o

37 s anticipated that similar thio-containing 4-methylumbelliferyl compounds will have applications in s

38 indicate that 7-diethylphospho-6,8-difluor-4-methylumbelliferyl (DEPFMU) is hydrolyzed specifically b

39 ees) describing the interaction of E2 with 4-methylumbelliferyl glycosides, determined by titrating t

40 characterized with a panel of fluorogenic, 4-methylumbelliferyl-linked substrate tests.

41 function is the fluorogenic reagent 2'-O-(4-methylumbelliferyl)-N-acetylneuraminic acid (MUN).

42 GH18(C) releases methylumbelliferone from 4'-methylumbelliferyl-N,N',N"-triacetylchitotriose 2.7-fold

43 uorescently labelled inhibitor, MeU-diNAG (4-methylumbelliferyl-N,N'-diacetyl-beta-D-chitobioside).

44 H18(N)) releases methylumbelliferone from 4'-methylumbelliferyl-N,N'-diacetylchitobiose 13.6-fold fas

45 With several probes (biotin, methylumbelliferyl-N-acetyl-alpha-D-neuraminic acid, and

46 me : ligand dissociation constants for three methylumbelliferyl oligosaccharides and cellotriose show

47 died using the fluorogenic ester substrate 4-methylumbelliferyl p'-guanidinobenzoate (MUGB).

48 we found that one substrate, 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP), was much improved

49 is of a fluorogenic substrate-6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP)-with a microplate

50 ere more than 10-fold higher than those of 4-methylumbelliferyl phosphate (MUP).

51 activity when measured using 6,8-difluoro-4-methylumbelliferyl phosphate as a fluorogenic substrate.

52 d by the dephosphorylation of 6,8-difluoro-4-methylumbelliferyl/phosphate to a fluorescent 6,8-difluo

53 easuring enzyme kinetics using fluorogenic 4-methylumbelliferyl substrates.

54 inophen sulfate (AS) and glucuronide (AG), 4-methylumbelliferyl sulfate (4MUS) and glucuronide (4MUG)

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