ライフサイエンスコーパス: microchip

1 in an effective length of 7 cm in a plastic microchip.

2 cate a two-dimensional ion-trap lattice on a microchip.

3 response and impedimetric measurement on the microchip.

4 pray supercharging) using a dual-sprayer ESI microchip.

5 w cells, inside the capillary column or on a microchip.

6 ng the fabricated liquid chromatography (LC) microchip.

7 ed voltage control in a miniaturized polymer microchip.

8 ectly from the corner of a rectangular glass microchip.

9 nd elution of nucleic acids in the polymeric microchip.

10 etic separations of proteins in a PMMA-based microchip.

11 rophoretic separation integrated on a single microchip.

12 rescence (SAF) microlens array embedded in a microchip.

13 lly integrated light-sources on conventional microchips.

14 ostructured Fabry-Perot interferometer (FPI) microchips.

15 added advantage of these PEG-functionalized microchips.

16 proteins and peptides with the PEG-modified microchips.

17 paration in poly(methyl methacrylate) (PMMA) microchips.

18 rials to construct capillary electrophoresis microchips.

19 t and fluidically complex multilayer polymer microchips.

20 ltifunctional windows for biofilm diagnostic microchips.

21 ntages over similar experiments performed on microchips.

22 complished using these new polymeric microCE microchips.

23 (dimethylsiloxane) capillary electrophoresis microchips.

24 to determine the analytical utility of these microchips.

25 pecifically for low-volume DNA extraction on microchips.

26 wn the size of electrodes in electroporation microchips.

27 The microchip, a commercial surface acoustic wave resonator,

28 replacing native PDMS microchips with hybrid microchips allowed the achievement of a 6-fold increase

29 measured for solutions introduced on a PDMS microchip allowing determination of forward and reverse

30 ble membranes has great potential to enhance microchip analysis of biomolecules.

31 e to fouling were obtained even after 1 h of microchip analysis with RSD values of </=4% and </=9% (n

32 vitro molecular imaging system comprising a microchip and a beta-particle imaging camera permitted r

33 n a poly(dimethylsiloxane) (PDMS) fabricated microchip and a common dc power supply.

34 le single-cell system that is comprised of a microchip and an adjustable clamp, so on-chip operation

35 In addition, we compared microchip and capillary electrophoresis under similar se

36 omechanical system fabricated from a silicon microchip and comprising a micromechanical resonator cou

37 SI-MS sensitivities were achieved using both microchip and conventional fused silica capillary emitte

38 et, which is generated by a heated nebulizer microchip and directed toward the mass spectrometer inle

39 thylsiloxane (PDMS)/glass chronoimpedimetric microchip and its removal in the same LOC system through

40 erature sensor was integrated into a silicon microchip and occupied 0.15 mm(2) of area.

41 The EWOD microchip and optimized synthesis method in combination

42 We used microChIP and qPCR assays of FACS-purified cells to trac

43 es with the simplicity of disposable polymer microchips and easy setup.

44 s with commercial microchips, wire imprinted microchips, and microchips from LIGA molding devices is

45 in DNA, wrote a 5.27-megabit book using DNA microchips, and read the book by using next-generation D

46 dentical spray geometries with both lamps in microchip APPI mass spectrometry (muAPPI-MS) and desorpt

47 Implementing our new streamlined microchip approach, we could directly visualize BRCA1 ge

48 As microchips are promising technical tools for a robust si

49 he results of designing and producing such a microchip array.

50 substance in plasma negatively affected the microchip assays and the effects could be minimized by d

51 simplicity, rapidity, and sensitivity of the microchip-assisted DNA extraction process.

52 The analytical performance of the microchip based assay was compared to that in the well p

53 were used to demonstrate the efficacy of the microchip based DNA extraction process.

54 lthough there are some approaches to develop microchip based pesticide detection platforms, there is

55 However, it has been challenging to realize microchip-based calorimeters possessing both high sensit

56 The microchip-based extraction is also performed in a closed

57 Microchip-based field asymmetric waveform ion mobility s

58 We utilized a novel microchip-based immunoaffinity capillary electrophoresis

59 ing the sensitivity and reproducibility of a microchip-based immunoassay by using isotachophoresis to

60 demonstrates the potential of incorporating microchip-based lipid extraction into cellular lipidomic

61 on, cytotoxicity, and contact dynamics using microchip-based live cell imaging.

62 RNA was purified using the microchip-based method from neat semen, a mock semen sta

63 DNA recovered was compatible with downstream microchip-based PCR amplification.

64 n capability, the arrayed nanostructured FPI microchip-based platform could provide an ideal technica

65 The microchip-based POC diagnostic demonstrated is applicabl

66 rived N-linked glycans with their subsequent microchip-based separation and mass-spectrometric (MS) m

67 -based extraction of DNA, we describe here a microchip-based solid-phase extraction method for purifi

68 our knowledge, this is the first report of a microchip-based system that couples microdialysis sampli

69 Here, we report a simple and low-cost microchip-based TB ELISA (MTBE) platform for the detecti

70 A microchip-based, displacement immunoassay for the sensit

71 Using a microchip-based, time-lapse imaging approach allowing th

72 ion mobility separations coupled with MS and microchip-based-proteome measurements combined with MS i

73 a testimony to the potential utility of PeT microchips beyond separations and presents a promising n

74 etric interference spectroscopy (RIfS) based microchip biosensor for the detection of circulating tum

75 tegrate an internal standard (ISTD) into the microchip by adding it to the background electrolyte (BG

76 compared to uninfected B cells and DEP-based microchips can be potentially used for sorting latently

77 Oligonucleotides from DNA microchips can reduce costs by at least an order of magn

78 In addition, these EFGF microchips can separate peptide samples with resolution

79 as oligonucleotides that were separated via microchip capillary electrochromatography (mu-CEC).

80 ns for microfabricated column structures for microchip capillary electrochromatography is presented.

81 OA), a portable instrument for the sensitive microchip capillary electrophoresis (CE) analysis of org

82 ation, and injection method is developed for microchip capillary electrophoresis (CE) and used to per

83 The Mars Organic Analyzer (MOA), a portable microchip capillary electrophoresis (CE) instrument deve

84 A microchip capillary electrophoresis (CE) separation with

85 A microchip capillary electrophoresis (MCE) system has bee

86 o improve point-of-care quantification using microchip capillary electrophoresis (MCE), the chip-to-c

87 evious reports describing sample stacking on microchip capillary electrophoresis (microCE) have regar

88 ed photopolymerized capture gel injector for microchip capillary electrophoresis (microCE).

89 ion, and injection of dsDNA for quantitative microchip capillary electrophoresis analysis.

90 me one of the most widely used materials for microchip capillary electrophoresis and microfluidics.

91 Furthermore, we present experimental microchip capillary electrophoresis measurements of inte

92 f trace amounts of amines and amino acids by microchip capillary electrophoresis on the Mars Organic

93 A dual-channel sequential injection microchip capillary electrophoresis system with pressure

94 ass transport of short interacting ssDNAs in microchip capillary electrophoresis.

95 of selenium oxoanions, we present the first microchip capillary zone electrophoresis (MCE) separatio

96 The device performance was evaluated using microchip capillary zone electrophoresis (mu-CZE) of ami

97 A hybrid microchip/capillary electrophoresis (CE) system was deve

98 es used in capillary electrophoresis (CE) to microchip CE (MCE) in order to improve concentration sen

99 PDMS, however, limits its applicability for microchip CE, microfluidic patterning, and other nonelec

100 his work, we demonstrate the utility of this microchip CE-ESI device for HX MS.

101 he results indicate the potential utility of microchip CE-ESI for HX MS.

102 The device has been used to perform microchip CE-MS analysis of peptides and proteins with e

103 ugation, the ADC was analyzed using the same microchip CE-MS method.

104 ions demonstrating the facile engineering of microchip-CE platforms for the analysis of a wide variet

105 electrophoresis (microCE) have regarded the microchip channels as a closed system and treated the bu

106 cy measured by chromatin immunoprecipitation-microchip (ChIP-chip) and ChIP-sequencing analyses.

107 Higher regression values obtained using microchip confirmed better quality and integrity of the

108 The E. coli reporter strain was filled in a microchip containing 16 independent electrochemical cell

109 The instrument uses a four-layer microchip, containing eight CE analysis systems integrat

110 Each microchip contains an array of discrete reservoirs from

111 The PPC microchip could also be used for subsequent assays with

112 zation and purification assay using this PPC microchip could be performed within approximately 25 min

113 lves a two-stage process to create the final microchip design.

114 R analysis and allows a compact, inexpensive microchip design.

115 We describe a microchip designed to quantify the levels of a dozen cyt

116 The glass microchip device consists of a microchannel that contain

117 The microchip device presented here is particularly suitable

118 A high-density multichannel microchip device was then fabricated and the microchanne

119 Using the microchip device, extraction efficiencies for lambda-pha

120 A home-made microchip electrophoresis (MCE) device was used to quant

121 Using droplet-interfaced microchip electrophoresis (MCE) techniques, we have deve

122 cs to couple multiwell plate-based assays to microchip electrophoresis (MCE) to screen enzyme modulat

123 that integrates microdialysis (MD) sampling, microchip electrophoresis (ME), and electrochemical dete

124 tem that couples microdialysis sampling with microchip electrophoresis and electrochemical detection.

125 We applied microchip electrophoresis and MALDI-TOF-MS-based glycomi

126 t improvement in the performance of PDMS for microchip electrophoresis and microfluidic applications.

127 th its unique capability to resolve isomers, microchip electrophoresis can yield complementary analyt

128 d by end-channel detection for capillary and microchip electrophoresis detection.

129 port is the first to describe a multichannel microchip electrophoresis device with integrated contact

130 Microchip electrophoresis is an emerging analytical tech

131 being developed to reduce sequencing costs, microchip electrophoresis is the only new technology rea

132 immunoaffinity purification step with rapid microchip electrophoresis separation in a laser-induced

133 nclusions about the role of order during the microchip electrophoresis separation of short DNA molecu

134 We have developed a microchip electrophoresis system that can automatically

135 ntervention; however, long-term operation of microchip electrophoresis systems has received little at

136 reveal the surprisingly powerful ability of microchip electrophoresis to provide ultrafast Sanger se

137 y between contact conductivity detection and microchip electrophoresis was developed.

138 equencing up to 600 bases in just 6.5 min by microchip electrophoresis with a unique polymer matrix/a

139 dysplasia, and esophageal adenocarcinoma by microchip electrophoresis with laser-induced fluorescenc

140 Microchip electrophoresis with two-photon excited fluore

141 be a novel class of DNA separation media for microchip electrophoresis, "physically cross-linked" blo

142 l and a triple-negative charge, separated by microchip electrophoresis, and detected by laser-induced

143 ole-cell catalyzed on-chip syntheses, chiral microchip electrophoresis, and label-free detection of e

144 ability of contact conductivity detection in microchip electrophoresis, and similar designs may have

145 an serum, we report a strategy that combines microchip electrophoresis, standard addition, enzymatic

146 With microchip electrophoresis, we are able to distinguish th

147 Through MS and microchip electrophoresis-based glycomic methods, severa

148 luding the narrow microchannels required for microchip electrophoresis.

149 2 nm for label-free analyte determination in microchip electrophoresis.

150 improved the performance of the devices for microchip electrophoresis.

151 ampling for sequential injection analysis by microchip electrophoresis.

152 matically performs the PCR amplification and microchip electrophoretic (ME) separation for rapid fore

153 The in-house fabricated microchips, electrophoretic protocols, and solution matr

154 A microchip electrospray emitter with a magnetic bead trap

155 A microchip electrospray ionization source was coupled wit

156 the metabolism of mitoxantrone is studied by microchip electrospray ionization-mass spectrometry.

157 e, and subsequently quantified the EVs via a microchip ELISA.

158 This attribute of the microchip emitter should simplify electrospray optimizat

159 000 nL/min) and applied potentials using the microchip emitters.

160 cated in the electric field-free region of a microchip, enabled the separation of six nitroaromatic a

161 In microchip EuNP immunoassay (microENIA) of inactivated in

162 nfluenza microENIA enhanced using the hybrid microchips even surpassed that of a commercial laborator

163 Effective microchip extraction of deoxyribonucleic acid (DNA) from

164 to approximately 25% of that obtained using microchips fabricated without MMA.

165 Microchip fabrication and integration of these pH sensor

166 Our microchip fabrication is simple and mass-producible as w

167 The microchip facilitated in-flow coating of chitosan on the

168 The poly(methyl methacrylate) (PMMA) microchips feature integral in-plane contactless conduct

169 A semipermeable copolymer in the EFGF microchips fills a region of changing cross-sectional ar

170 t-of-detection of current nanostructured FPI microchip for f-PSA is about 10 pg/mL and the upper dete

171 Here, we report a microchip for rapid (<1h) detection of P. aeruginosa (62

172 -based continuous flow thermal cycler (CFTC) microchip for Sanger cycle sequencing using dye terminat

173 substrates have been used for fabrication of microchips for DNA extraction, PCR amplification, and DN

174 onstrate the effectiveness of multilayer PeT microchips for dynamic solid phase extraction (dSPE) and

175 rane arrays in poly(dimethylsiloxane) (PDMS) microchips for label-free analysis of lipid-protein inte

176 a route to the creation of three-dimensional microchips for memory and logic applications.

177 l microchips, wire imprinted microchips, and microchips from LIGA molding devices is presented.

178 To fabrication through-hole microchips from this alternative material for microfluid

179 The concept can be extended to other microchip functions and stimuli-responsive materials and

180 old reduction in peak width in the following microchip gradient LC separation.

181 A microchip has been fabricated to perform the analysis.

182 ynthesis methods harnessing the power of DNA microchips have recently been demonstrated.

183 SA tryptic digest are demonstrated using the microchip HPLC system.

184 ntegrate the nanoporous-junction into a PDMS microchip in a leak-free manner with excellent repeatabi

185 buffers to all 40 reservoirs situated on the microchip in only five pipetting steps using an 8-channe

186 gh quality DNA rapidly, we have fabricated a microchip in poly(dimethyl siloxane) (PDMS) by soft lith

187 s a performance bottleneck for semiconductor microchips in modern computer systems--from mobile phone

188 ent multielectrochemical electrode arrays on microchips in order to automate measurement of quantal e

189 and mechanical resonator formed in a silicon microchip, in which radiation pressure from a laser is u

190 ch to the rapid fabrication of rigid plastic microchips including the narrow microchannels required f

191 The microchip incorporated a 30-mm SDS micro-CGE and a 10-mm

192 volumes could be achieved by using the PDMS microchip injector.

193 ay variation was clearly reduced when hybrid microchips instead of native PDMS microchips were used i

194 The microchip integrates this precolumn derivatization, cont

195 Efficient microchip integration of these processes enables the sen

196 a robust, integrated poly(dimethylsiloxane) microchip interface for ESI-MS using simple and widely a

197 This microchip is adaptable to all commercial TEM holders.

198 The microchip is capable of robust local temperature control

199 The microchip is made of two bare gold electrodes and PDMS m

200 Development of affordable microchip laser sources has the potential to substantial

201 erimental conditions, the performance of the microchip LC was comparable to that obtained with bencht

202 des using titanium dioxide incorporated in a microchip liquid chromatography device was used to analy

203 solid-phase reversible immobilization (SPRI) microchip made from PC for purification of the DNA seque

204 e same chip has proved challenging, owing to microchip manufacturing conflicts between electronics an

205 icrofluidic cell culture device created with microchip manufacturing methods that contains continuous

206 ly cleaved from the antibody and analyzed by microchip MEKC.

207 posomal and polymeric nanoparticles, wafers, microchips, microparticle-based nanoplatforms and cells-

208 retic separation performance attainable from microchips molded by masters fabricated using convention

209 rtant limitations of nonsilicon and nonglass microchips, namely, resistance to nonpolar solvents and

210 , demonstrating the potential of this unique microchip nanoparticle assay in clinical diagnosis of in

211 Here, we present a microchip nanopipet with a narrow chamber width for sort

212 es of a new, high-repetition-rate (36.6 kHz) microchip Nd:YAG laser.

213 Through miniaturization into microchips, new techniques have been realized that would

214 We demonstrate microchip nonaqueous capillary electrophoresis (muNACE)

215 surements were evaluated indicating that the microchip packed with fresh silica beads is capable of b

216 quality was maintained when integrated with microchip PCR.

217 under similar separation conditions, and the microchips performed as well as the capillaries.

218 t was found that detection capability of the microchip platform could be readily improved using Europ

219 Meanwhile, use of the microchip platform effectively reduced sample/reagent co

220 n and incubation time, were optimized in the microchip platform for the lowest limit of detection, hi

221 we continued to miniaturize this assay to a microchip platform for the purpose of converting the ben

222 We have also evaluated the microchip platform with discarded, de-identified HIV-inf

223 ased DNA detection technology scalable for a microchip platform, we describe a simplistic, low-cost m

224 e successfully tested using muENIA on hybrid microchip platforms, demonstrating the potential of this

225 The microchip process was accomplished in sub-90 min compare

226 es obtained from the data suggested that the microchip process was reproducible.

227 e region II amplicon was introduced into the microchip, purified, concentrated, and injected, generat

228 the need for additional sample manipulation, microchip redesigns, and/or system expansions required f

229 in the downstream, field-free region of the microchip, resulting in a stationary-phase monolith with

230 further validated our assay by comparing our microchip results with the standard culture-based method

231 consists of a poly(dimethylsiloxane) (PDMS) microchip sample injector featuring a pneumatic microval

232 low, permitting inertial collection into the microchip sample reservoir.

233 Here we use high-fidelity DNA microchips, selective oligonucleotide pool amplification

234 Applying two-photon excitation microchip separations and label-free detection could als

235 e block copolymers specifically designed for microchip separations to achieve accurate DNA size calli

236 Coupling of the CFTC chip to the SPRI microchip showed read lengths similar to that obtained f

237 ms relative to the existing state-of-the-art microchip source and demonstrate its utility for creatin

238 The microchip subassays were optimized to deliver results co

239 Escherichia coli using a highly error-prone microchip-synthesized oligo pool (479 oligos) without pr

240 ach for engineering a synthetic pathway with microchip-synthesized oligonucleotides (oligo).

241 nomical de novo gene synthesis methods using microchip-synthesized oligonucleotides has been limited

242 e high-quality DNA from oligos, particularly microchip-synthesized oligonucleotides.

243 ults indicate that the prototype growth tube-microchip system (termed aerosol chip electrophoresis, A

244 rrent paradigm of capillary electrophoresis, microchip systems promise to reduce sequencing costs dra

245 uantifying the extent of interaction in this microchip technique may prove powerful for exploration o

246 Here we developed a microchip technology (the Cluster-Chip) to capture CTC c

247 uNWs approach, enhancing the maturity of the microchip technology and opening new avenues for future

248 We evaluated the microchip technology by detecting and quantifying multip

249 We used a microchip technology that facilitates quantification of

250 By using an integrated microchip that can programmably control the solution pH

251 ntegrated selective enrichment target (ISET) microchip that improves the sample preparation step for

252 ation, we developed a splittable single-cell microchip that integrates a high-density antibody array

253 oteins are separated by electrophoresis on a microchip that is dragged along a polyvinylidene fluorid

254 ylene glycol) (PEG)-functionalized polymeric microchips that are inherently resistant to protein adso

255 The system employs tunable microchips that can be decorated with switchable adaptor

256 nano-SPEARs provide the core technology for microchips that enable scalable, in vivo studies of neur

257 sis technology, which integrates on a single microchip the synthesis of DNA oligonucleotides using in

258 to most methods used to fabricate polymeric microchips, the photopolymerization-based method used wi

259 ve and passive detection states to allow the microchip to be periodically activated to perform a meas

260 The absence of band broadening from microchip to capillary indicated a minimum dead volume a

261 and fluorescein (FL), were performed on PMMA microchips to demonstrate the feasibility of the fabrica

262 he fabrication technique was tested to build microchips to perform several analyses, including chroma

263 The complete isolation of the DNA using the microchip took 15min as against>2h with a TRIzol metho

264 oped system was very robust, with individual microchips used for up to 2000 analyses with lifetimes l

265 nate (PC)-polydimethylsiloxane (PDMS) hybrid microchip using a simple epoxy silica sol-gel coating/bo

266 ds for fabricating poly(methyl methacrylate) microchips using a novel two-stage embossing technique a

267 n optical fibers, which were placed into the microchip, using guides at the outlet of the flow, incre

268 The microchips utilized consist of a flow-through silicon pl

269 IV-infected patient samples by comparing our microchip viral load measurement results with reverse tr

270 layer and a blank PMMA layer to generate the microchip was achieved by solvent welding.

271 trate the potential uses of this device, the microchip was coupled to a microdialysis probe to monito

272 rporation of solid-phase extraction (SPE) to microchip was ensured by facile 3D element integration u

273 interference from the sampling pressure, the microchip was operated isobarically by sealing the buffe

274 The microchip was prepared by hot embossing into PMMA from a

275 DNA extracted using the microchip was pure with absorbance (260/280) ratio of 1.

276 The CFTC microchip was subsequently coupled to a solid-phase reve

277 rs, the LOD of influenza microENIA on hybrid microchips was determined to be ~10(4) TCID50 titer/mL a

278 Using temperature transponder microchips, we showed that the core body temperature inc

279 The microchips were characterized with regard to pH sensitiv

280 Resultant improved microchips were evaluated for the separation of fluoresc

281 Four tested microchips were measured to consume only 113 pW with a r

282 horetic separations with membrane-containing microchips were performed on cations, anions, and amino

283 These microchips were used for FFIEF of small molecule markers

284 hen hybrid microchips instead of native PDMS microchips were used in the microENIA tests.

285 nd-channel electrochemical detection on this microchip, where an electrophoretic separation of dopami

286 f the assay, a cyclic olefin copolymer (COC) microchip, which was fabricated using hot embossing, was

287 orescently labeled cells were immobilized on microchip window surfaces and visualized in a fluid cell

288 nal CNC machining techniques with commercial microchips, wire imprinted microchips, and microchips fr

289 ting extraction of glycerophospholipids on a microchip with a nanoelectrospray ionization quadrupole

290 Here we show that an endothelialized microchip with controllable permeability can be used to

291 We design and fabricate a microchip with integrated analyte injection and detectio

292 cal sensing of viruses on a flexible plastic microchip with printed electrodes.

293 We evaluated the microchip with spiked samples of PBS with bacteria conce

294 here, we established 61 kV/cm in N(2) using microchips with 35 microm gaps.

295 xperiments were performed using fused silica microchips with and without octadecyltrimethoxysilane co

296 ted influenza viruses, replacing native PDMS microchips with hybrid microchips allowed the achievemen

297 es have progressed on many fronts, servicing microchips with minute amounts of reagents still constit

298 The microchips with the microelectrode array were fabricated

299 Illustrative of the compatibility of PeT microchips with the PCR process, the successful amplific

300 This method utilizes microchip zone electrophoresis for rapid separation (<90