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1 We recently described Tudor-staphylococcal/micrococcal-like nuclease (TSN)-mediated miRNA decay (Tu
2 c mRNA length) were found to be resistant to micrococcal nuclease (69%) or to remain suspended in ass
3 nization but were found to be susceptible to micrococcal nuclease (85%) or to sediment to a pellet in
7 ly in situ single cell chromatin imaging and micrococcal nuclease (MNase) assay to show that Brd4 dep
8 ructure of chromatin in cereal species using micrococcal nuclease (MNase) cleavage showed nucleosomal
11 accessibility of nucleosomes, as measured by micrococcal nuclease (MNase) digestion and ATAC-seq (ass
12 uch as chromatin immunoprecipitation (ChIP), micrococcal nuclease (MNase) digestion and DNase I diges
14 emodelling analysis at gene promoters, using micrococcal nuclease (MNase) digestion followed by deep
18 erential nuclease sensitivity assay based on micrococcal nuclease (MNase) digestion to discover open
19 al DNA was significantly less protected from micrococcal nuclease (MNase) digestion up to 6 h postinf
20 erepressed ESs show increased sensitivity to micrococcal nuclease (MNase) digestion, and a decrease i
24 ely digested to mononucleosomes using either micrococcal nuclease (MNase) or caspase-activated DNase
25 re-RC) assembly, replication initiation, and micrococcal nuclease (MNase) sensitivity at different ce
28 ome-wide mapping of nucleosomes generated by micrococcal nuclease (MNase) suggests that yeast promote
29 method to measure chromatin accessibility to micrococcal nuclease (MNase) that is normalized for nucl
30 hEC) uses fusion of a protein of interest to micrococcal nuclease (MNase) to target calcium-dependent
32 In this review, we compare the traditional micrococcal nuclease (MNase)-based approach with a chemi
34 bind one or both full nucleosomes that flank micrococcal nuclease (MNase)-defined nucleosome-free pro
42 sults demonstrate increased DNA laddering by micrococcal nuclease and an increased amount of DNA inte
43 units are equally accessible to DNase I and micrococcal nuclease and contain similar levels of histo
44 n vitro and in vivo assays of sensitivity to micrococcal nuclease and dam methyltransferase, respecti
45 tivity of wild-type nuclei to digestion with micrococcal nuclease and deoxyribonuclease I, indicating
46 ecreased accessibility of their chromatin to micrococcal nuclease and DNase I digestion and increased
47 s of chromatin structure by accessibility to micrococcal nuclease and DNase I digestion demonstrated
49 T118-I) are more accessible to digestion by micrococcal nuclease and do not constrain DNA in a preci
50 s with defined ends, bulk NCPs prepared with micrococcal nuclease and molecular modelling to reassess
51 s no hypersensitivity to either DNase I or a micrococcal nuclease and no translational positioning of
53 e of ribosomal chromatin was investigated by micrococcal nuclease and psoralen photocrosslinking.
54 undaries were determined by assays combining micrococcal nuclease and restriction endonuclease digest
55 region for several days, as demonstrated by micrococcal nuclease and restriction enzyme accessibilit
56 tuted mononucleosomes using exonuclease III, micrococcal nuclease and restriction enzymes demonstrate
58 erformance liquid chromatography analysis of micrococcal nuclease and spleen phosphodiesterase-digest
60 eabilization and digestion of chromatin with micrococcal nuclease and then compared tumor necrosis fa
61 e flexibility and strongly blocked access of micrococcal nuclease as contour lengths shortened, consi
62 om human metaphase chromosomes digested with micrococcal nuclease associate spontaneously forming mul
64 osome leading to an asymmetric protection to micrococcal nuclease cleavage of linker DNA relative to
65 itional perturbation is marked by changes in micrococcal nuclease cleavage patterns, restriction endo
66 chromatin nor to differences in the in vivo micrococcal nuclease cleavage sites in individual genes
67 10-bp periodicity in WW dinucleotides and in micrococcal nuclease cleavage, providing evidence for ro
69 clei sorting; 3) preparation of chromatin by micrococcal nuclease digest; 4) ChIP for open chromatin-
71 gradient sedimentation, thermal disassembly, micrococcal nuclease digestion and atomic force microsco
72 examined HSV-1 during lytic infection using micrococcal nuclease digestion and chromatin immunopreci
73 of S. cerevisiae nucleosome lengths based on micrococcal nuclease digestion and paired-end sequencing
74 er DNA, stabilizing an additional 20 bp from micrococcal nuclease digestion and restrict nucleosome m
75 e demonstrate by single-molecule approaches, micrococcal nuclease digestion and small-angle X-ray sca
76 e center of the DNA sequence, protected from micrococcal nuclease digestion by incorporation into a p
77 HBc 149, 154, and 157) remained intact after micrococcal nuclease digestion by native gel electrophor
78 region of the capsid pgRNA is susceptible to micrococcal nuclease digestion during its isolation and
79 replicative aging using spike-in controlled micrococcal nuclease digestion followed by sequencing.
80 ammalian linker histone H1 and have a unique micrococcal nuclease digestion footprint that allows the
81 ound histone H3 and increased sensitivity to micrococcal nuclease digestion in WHS patient-derived ce
84 itivity of bulk chromatin from sin4 cells to micrococcal nuclease digestion is strikingly increased r
86 of genomic DNA species, produced by partial micrococcal nuclease digestion of chromatin, can be sequ
89 itation, we developed a novel strategy using micrococcal nuclease digestion of cross-linked chromatin
91 445 nucleotide human telomerase RNA (hTR) by micrococcal nuclease digestion of partially purified hum
92 In this study, we use a procedure based on micrococcal nuclease digestion of reconstituted nucleoso
94 pause at approximately 168 base pairs in the micrococcal nuclease digestion pattern of the chromatin.
96 rther, we found that A-T cells had different micrococcal nuclease digestion patterns compared to norm
99 from the Rb-/- cells is more susceptible to micrococcal nuclease digestion than that from Rb+/+ fibr
100 the exon 1 region is much more sensitive to micrococcal nuclease digestion than the exon 2 and exon
102 obes were used to capture RNA targets, and a micrococcal nuclease digestion was performed to remove a
104 nucleosome position in follicle cells using micrococcal nuclease digestion with Ilumina sequencing.
105 and loss of a regular nucleosomal ladder on micrococcal nuclease digestion, addition of TSA relieves
106 leosomes, produce a chromatosome stop during micrococcal nuclease digestion, and aggregate chromatin.
107 NA and multiples of approximately 60 bp from micrococcal nuclease digestion, and immunoprecipitation
108 nucleosomes prepared by partial and maximum micrococcal nuclease digestion, coupled with Western blo
109 niques along with such laboratory methods as micrococcal nuclease digestion, predicting the genomic l
110 H3 nucleosomes protect 90-100 bp of DNA from micrococcal nuclease digestion, sufficient for only a si
111 some maps generated by chemical cleavage and micrococcal nuclease digestion, the chemical map shows c
119 size differences between repeats in partial micrococcal nuclease digests and by trypsin treatment of
121 a typical pattern of nucleosomal repeats in micrococcal nuclease digests, the Tec element chromatin
123 rmal global chromatin density as assessed by micrococcal nuclease digests; and expressed normal level
124 e)--a protein containing five staphylococcal/micrococcal nuclease domains and a tudor domain--is a co
126 tivity () was solubilized by Triton X-100 or micrococcal nuclease extraction, whereas hTSH2B was rela
130 examined salt-soluble chromatin released by micrococcal nuclease from a 15-day-old chicken embryo er
131 ested chromatin from untreated rats revealed micrococcal nuclease hypersensitive regions in the proxi
133 ional responses to cold tend to contain more micrococcal nuclease hypersensitive sites in their promo
134 ma regions undergoes dramatic alterations in micrococcal nuclease hypersensitivity as cells cross the
139 P knockout) brain homogenate with RNase A or micrococcal nuclease inhibited hamster but not mouse PrP
140 ocalization of Cse4 in chromatin digested by micrococcal nuclease is consistent with the potential as
141 cribe a Hi-C-based method, Micro-C, in which micrococcal nuclease is used instead of restriction enzy
142 reconstituted branch migration substrates by micrococcal nuclease mapping and exonuclease III and hyd
149 moderately greater sequence preference than micrococcal nuclease or DNase I, and the sites attacked
151 prepared from chromatin digested with either micrococcal nuclease or DNaseI and are restricted in the
152 th Cse4 and H2A are precisely protected from micrococcal nuclease over the entire CDE of all 16 yeast
157 nt stagger is explained by the finding that micrococcal nuclease produces NCPs not with flush ends,
159 ich antibody-targeted controlled cleavage by micrococcal nuclease releases specific protein-DNA compl
160 ive and inactive CEN chromatin digested with micrococcal nuclease revealed that periodic nucleosome a
161 e of exons 1, 2, and 5 of the DHFR gene with micrococcal nuclease revealed that the exon 1 region is
165 pressed CFS, the FRA3B, is more resistant to micrococcal nuclease than that of the flanking non-fragi
167 a cell extracts, which were pre-treated with micrococcal nuclease to degrade the endogenous RNase P R
168 riments where the extracts were treated with micrococcal nuclease to digest endogenous snRNAs, the ef
169 acO/GFP-LacI plants were lysed, treated with micrococcal nuclease to digest the DNA to fragments of a
170 that the 3' ends of capsid pgRNA isolated by micrococcal nuclease treatment are heterogeneously dispe
171 ted by using an established method involving micrococcal nuclease treatment demonstrated reduced leve
172 as not bridged by nucleic acids, as shown by micrococcal nuclease treatment of the proteins prior to
173 eosome cores were liberated using an enzyme (micrococcal nuclease) with a strong preference for cleav
174 vage activity is sensitive to treatment with micrococcal nuclease, also consistent with an activity a
176 digestion by DNAse, restriction enzymes, and micrococcal nuclease, and an increased affinity for GAL4
179 atin in the spt6 mutant is hypersensitive to micrococcal nuclease, and this hypersensitivity is suppr
181 f nuclease probes including exonuclease III, micrococcal nuclease, DNase I, and restriction enzymes.
183 histone promoters and transcribed regions to micrococcal nuclease, implicating UBTF1/2 in mediating D
184 DNA and stabilize it against digestion with micrococcal nuclease, in a similar manner to histone H1.
185 fragility, manifested as high sensitivity to micrococcal nuclease, in contrast to the common presumpt
186 ith increased resistance to both DNase I and micrococcal nuclease, indicating that the silenced state
187 mic DNA is either sonicated or digested with micrococcal nuclease, making it possible that current pr
188 chromatic sequences become hypersensitive to micrococcal nuclease, nucleoli fail to form, and transcr
189 deed induce sites hypersensitive to DNase I, micrococcal nuclease, or restriction enzymes on either s
191 table for analysis of chromatin structure by micrococcal nuclease, restriction endonuclease or by imm
192 A (vRNA) has been depleted by treatment with micrococcal nuclease, was used to study transcription in
198 letion of both tails, a lethal event, alters micrococcal nuclease-generated nucleosomal ladders, plas
199 adjacent to the TATAA box and an additional micrococcal nuclease-hypersensitive site in the linker D
201 ent protein-tagged H1 variants, we show that micrococcal nuclease-resistant chromatin is specifically
202 ift assay, this protein produced a discrete, micrococcal nuclease-resistant complex with an approxima
205 s that the centromeric nucleosome contains a micrococcal nuclease-resistant kernel of 123-135 bp, dep
206 ementing activity was protease-sensitive and micrococcal nuclease-resistant, had a native molecular m
207 nctional significance of this association, a micrococcal nuclease-sensitive component, i.e., an snRNP
212 by incubating human 32P-labeled U2 snRNP in micrococcal nuclease-treated HeLa nuclear extracts, foll
229 sed sensitivity of chromatin to digestion by micrococcal nuclease; however, phosphorylation of H2A an
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