ライフサイエンスコーパス: micrococcal nuclease

1 p of DNA is protected against digestion with micrococcal nuclease.

2 bited by a specific competitive inhibitor of micrococcal nuclease.

3 DNA fragments resulting from digestions with micrococcal nuclease.

4 the sensitivity of chromatin to digestion by micrococcal nuclease.

5 al DNA ladders sharper than those created by micrococcal nuclease.

6 d treatment of lysates with Ca(2+)-dependent micrococcal nuclease.

7 s even after digestion of the chromatin with micrococcal nuclease.

8 component is sensitive to cycloheximide and micrococcal nuclease.

9 iation to treatment with ethidium bromide or micrococcal nuclease.

10 decreased rate of digestion of chromatin by micrococcal nuclease.

11 ated by treatment with proteinase K, but not micrococcal nuclease.

12 leosomal DNA to Dam methyltransferase and to micrococcal nuclease.

13 the primary chromatin structure is probed by micrococcal nuclease.

14 tects approximately 11 bp of linker DNA from micrococcal nuclease.

15 c mRNA length) were found to be resistant to micrococcal nuclease (69%) or to remain suspended in ass

16 nization but were found to be susceptible to micrococcal nuclease (85%) or to sediment to a pellet in

17 Pretreatment of the U1 snRNP with micrococcal nuclease abolished the assembly of galectin-

18 vage activity is sensitive to treatment with micrococcal nuclease, also consistent with an activity a

19 Linker DNA was freely accessible to micrococcal nuclease, although the oligomers remained pa

20 High-resolution micrococcal nuclease analyses revealed that a positioned

21 Micrococcal nuclease analysis revealed that the heteroch

22 sults demonstrate increased DNA laddering by micrococcal nuclease and an increased amount of DNA inte

23 units are equally accessible to DNase I and micrococcal nuclease and contain similar levels of histo

24 n vitro and in vivo assays of sensitivity to micrococcal nuclease and dam methyltransferase, respecti

25 tivity of wild-type nuclei to digestion with micrococcal nuclease and deoxyribonuclease I, indicating

26 ecreased accessibility of their chromatin to micrococcal nuclease and DNase I digestion and increased

27 s of chromatin structure by accessibility to micrococcal nuclease and DNase I digestion demonstrated

28 core region of the insulator as revealed by micrococcal nuclease and DNase I digestion studies.

29 T118-I) are more accessible to digestion by micrococcal nuclease and do not constrain DNA in a preci

30 s with defined ends, bulk NCPs prepared with micrococcal nuclease and molecular modelling to reassess

31 s no hypersensitivity to either DNase I or a micrococcal nuclease and no translational positioning of

32 complex, as supported by its sensitivity to micrococcal nuclease and proteinase K.

33 e of ribosomal chromatin was investigated by micrococcal nuclease and psoralen photocrosslinking.

34 undaries were determined by assays combining micrococcal nuclease and restriction endonuclease digest

35 region for several days, as demonstrated by micrococcal nuclease and restriction enzyme accessibilit

36 tuted mononucleosomes using exonuclease III, micrococcal nuclease and restriction enzymes demonstrate

37 Micrococcal nuclease and short-recognition-sequence blun

38 erformance liquid chromatography analysis of micrococcal nuclease and spleen phosphodiesterase-digest

39 In micrococcal nuclease and supercoiling assays, addition o

40 eabilization and digestion of chromatin with micrococcal nuclease and then compared tumor necrosis fa

41 digestion by DNAse, restriction enzymes, and micrococcal nuclease, and an increased affinity for GAL4

42 DNase I, micrococcal nuclease, and exonuclease III footprinting s

43 By combining DNase I, micrococcal nuclease, and specific restriction endonucle

44 atin in the spt6 mutant is hypersensitive to micrococcal nuclease, and this hypersensitivity is suppr

45 e flexibility and strongly blocked access of micrococcal nuclease as contour lengths shortened, consi

46 om human metaphase chromosomes digested with micrococcal nuclease associate spontaneously forming mul

47 The RTC becomes permeable to micrococcal nuclease but not to antibodies.

48 osome leading to an asymmetric protection to micrococcal nuclease cleavage of linker DNA relative to

49 itional perturbation is marked by changes in micrococcal nuclease cleavage patterns, restriction endo

50 chromatin nor to differences in the in vivo micrococcal nuclease cleavage sites in individual genes

51 10-bp periodicity in WW dinucleotides and in micrococcal nuclease cleavage, providing evidence for ro

52 caused by either linker histone addition or micrococcal nuclease cleavage.

53 clei sorting; 3) preparation of chromatin by micrococcal nuclease digest; 4) ChIP for open chromatin-

54 Separation of micrococcal nuclease-digested chromatin by sucrose gradi

55 Low resolution Southern analysis of micrococcal nuclease-digested chromatin from untreated r

56 We begin with micrococcal nuclease-digested non-cross-linked chromatin

57 The protection against micrococcal nuclease digestion afforded to chromatosomal

58 gradient sedimentation, thermal disassembly, micrococcal nuclease digestion and atomic force microsco

59 examined HSV-1 during lytic infection using micrococcal nuclease digestion and chromatin immunopreci

60 of S. cerevisiae nucleosome lengths based on micrococcal nuclease digestion and paired-end sequencing

61 er DNA, stabilizing an additional 20 bp from micrococcal nuclease digestion and restrict nucleosome m

62 e demonstrate by single-molecule approaches, micrococcal nuclease digestion and small-angle X-ray sca

63 e center of the DNA sequence, protected from micrococcal nuclease digestion by incorporation into a p

64 HBc 149, 154, and 157) remained intact after micrococcal nuclease digestion by native gel electrophor

65 region of the capsid pgRNA is susceptible to micrococcal nuclease digestion during its isolation and

66 replicative aging using spike-in controlled micrococcal nuclease digestion followed by sequencing.

67 ammalian linker histone H1 and have a unique micrococcal nuclease digestion footprint that allows the

68 ound histone H3 and increased sensitivity to micrococcal nuclease digestion in WHS patient-derived ce

69 Partial micrococcal nuclease digestion indicated that the sequen

70 Micrococcal nuclease digestion indicated the presence of

71 itivity of bulk chromatin from sin4 cells to micrococcal nuclease digestion is strikingly increased r

72 Micrococcal nuclease digestion of active promoters in nu

73 of genomic DNA species, produced by partial micrococcal nuclease digestion of chromatin, can be sequ

74 of a 166 bp chromatosome intermediate during micrococcal nuclease digestion of chromatin.

75 me-length DNA ( approximately 166 bp) during micrococcal nuclease digestion of chromatin.

76 itation, we developed a novel strategy using micrococcal nuclease digestion of cross-linked chromatin

77 Capsid disassembly can be induced by micrococcal nuclease digestion of encapsidated RNA.

78 445 nucleotide human telomerase RNA (hTR) by micrococcal nuclease digestion of partially purified hum

79 In this study, we use a procedure based on micrococcal nuclease digestion of reconstituted nucleoso

80 The micrococcal nuclease digestion pattern of chromatin from

81 pause at approximately 168 base pairs in the micrococcal nuclease digestion pattern of the chromatin.

82 ure on the transient template as measured by micrococcal nuclease digestion pattern.

83 rther, we found that A-T cells had different micrococcal nuclease digestion patterns compared to norm

84 structure as DNaseI-hypersensitive sites or micrococcal nuclease digestion patterns.

85 Despite these changes, the micrococcal nuclease digestion profile of this promoter

86 from the Rb-/- cells is more susceptible to micrococcal nuclease digestion than that from Rb+/+ fibr

87 the exon 1 region is much more sensitive to micrococcal nuclease digestion than the exon 2 and exon

88 A major site of micrococcal nuclease digestion was identified by mapping

89 obes were used to capture RNA targets, and a micrococcal nuclease digestion was performed to remove a

90 Similar kinetics of micrococcal nuclease digestion were seen for all three t

91 nucleosome position in follicle cells using micrococcal nuclease digestion with Ilumina sequencing.

92 and loss of a regular nucleosomal ladder on micrococcal nuclease digestion, addition of TSA relieves

93 leosomes, produce a chromatosome stop during micrococcal nuclease digestion, and aggregate chromatin.

94 NA and multiples of approximately 60 bp from micrococcal nuclease digestion, and immunoprecipitation

95 nucleosomes prepared by partial and maximum micrococcal nuclease digestion, coupled with Western blo

96 niques along with such laboratory methods as micrococcal nuclease digestion, predicting the genomic l

97 H3 nucleosomes protect 90-100 bp of DNA from micrococcal nuclease digestion, sufficient for only a si

98 some maps generated by chemical cleavage and micrococcal nuclease digestion, the chemical map shows c

99 Using micrococcal nuclease digestion, we probed the chromatin

100 CTD antibodies from chromatin solubilized by micrococcal nuclease digestion.

101 ved from K562 human erythroleukemic cells by micrococcal nuclease digestion.

102 . thermophila mononucleosomes were stable to micrococcal nuclease digestion.

103 ity, and chromatin that is hypersensitive to micrococcal nuclease digestion.

104 ome segment protected by the polymerase from micrococcal nuclease digestion.

105 chromatin results in increased resistance to micrococcal nuclease digestion.

106 In micrococcal-nuclease digestion experiments, nucleosomes

107 size differences between repeats in partial micrococcal nuclease digests and by trypsin treatment of

108 Analysis of micrococcal nuclease digests of chromatin using hybridiz

109 a typical pattern of nucleosomal repeats in micrococcal nuclease digests, the Tec element chromatin

110 by Snf-Swi, by a high resolution analysis of micrococcal nuclease digests.

111 rmal global chromatin density as assessed by micrococcal nuclease digests; and expressed normal level

112 Micrococcal nuclease, DNase I, and restriction enzymes s

113 f nuclease probes including exonuclease III, micrococcal nuclease, DNase I, and restriction enzymes.

114 e)--a protein containing five staphylococcal/micrococcal nuclease domains and a tudor domain--is a co

115 Pretreatment of nuclear extracts with micrococcal nuclease eliminated the phosphorylation of C

116 tivity () was solubilized by Triton X-100 or micrococcal nuclease extraction, whereas hTSH2B was rela

117 osomal protections in Drosophila cells using Micrococcal Nuclease followed by sequencing.

118 Micrococcal nuclease footprinting shows that after track

119 DNase I and micrococcal nuclease footprinting, of both 5' and 3' 32P

120 examined salt-soluble chromatin released by micrococcal nuclease from a 15-day-old chicken embryo er

121 letion of both tails, a lethal event, alters micrococcal nuclease-generated nucleosomal ladders, plas

122 sed sensitivity of chromatin to digestion by micrococcal nuclease; however, phosphorylation of H2A an

123 ested chromatin from untreated rats revealed micrococcal nuclease hypersensitive regions in the proxi

124 The TBP gene comprises a 220 bp micrococcal nuclease hypersensitive site corresponding t

125 ional responses to cold tend to contain more micrococcal nuclease hypersensitive sites in their promo

126 adjacent to the TATAA box and an additional micrococcal nuclease-hypersensitive site in the linker D

127 The position and intensity of micrococcal nuclease-hypersensitive sites correlate poor

128 ma regions undergoes dramatic alterations in micrococcal nuclease hypersensitivity as cells cross the

129 Both hotspot sequences were sites of micrococcal nuclease hypersensitivity in meiotic chromat

130 Micrococcal nuclease hypersensitivity in the PBRU was la

131 We report DNase I and micrococcal nuclease hypersensitivity, chromatin immunop

132 s associated with increased accessibility to micrococcal nuclease, i.e. nucleosome depletion.

133 histone promoters and transcribed regions to micrococcal nuclease, implicating UBTF1/2 in mediating D

134 The chromatin accessibility to micrococcal nuclease in the MFA2 promoter is unaffected

135 DNA and stabilize it against digestion with micrococcal nuclease, in a similar manner to histone H1.

136 fragility, manifested as high sensitivity to micrococcal nuclease, in contrast to the common presumpt

137 ith increased resistance to both DNase I and micrococcal nuclease, indicating that the silenced state

138 P knockout) brain homogenate with RNase A or micrococcal nuclease inhibited hamster but not mouse PrP

139 ocalization of Cse4 in chromatin digested by micrococcal nuclease is consistent with the potential as

140 cribe a Hi-C-based method, Micro-C, in which micrococcal nuclease is used instead of restriction enzy

141 mic DNA is either sonicated or digested with micrococcal nuclease, making it possible that current pr

142 reconstituted branch migration substrates by micrococcal nuclease mapping and exonuclease III and hyd

143 Chromatin immunoprecipitation and micrococcal nuclease mapping studies reveal that Knirps

144 Micrococcal nuclease mapping studies revealed that a nuc

145 With high-resolution micrococcal nuclease mapping, we show that the HMRa locu

146 r, since the activities in the extracts were micrococcal nuclease (MN) sensitive.

147 scription elongation complex to digestion by micrococcal nuclease (MN).

148 activity of the S. aureus secreted nuclease, micrococcal nuclease (MN).

149 ly in situ single cell chromatin imaging and micrococcal nuclease (MNase) assay to show that Brd4 dep

150 ructure of chromatin in cereal species using micrococcal nuclease (MNase) cleavage showed nucleosomal

151 Chromatin immunoprecipitation (ChIP) and micrococcal nuclease (MNase) digest assays were performe

152 We combined low and high levels of micrococcal nuclease (MNase) digestion along with core h

153 accessibility of nucleosomes, as measured by micrococcal nuclease (MNase) digestion and ATAC-seq (ass

154 uch as chromatin immunoprecipitation (ChIP), micrococcal nuclease (MNase) digestion and DNase I diges

155 We performed a time-course of micrococcal nuclease (MNase) digestion and measured the

156 emodelling analysis at gene promoters, using micrococcal nuclease (MNase) digestion followed by deep

157 We have used micrococcal nuclease (MNase) digestion followed by deep

158 We introduce a metric that uses micrococcal nuclease (MNase) digestion in a novel manner

159 We have combined standard micrococcal nuclease (MNase) digestion of nuclei with a

160 erential nuclease sensitivity assay based on micrococcal nuclease (MNase) digestion to discover open

161 al DNA was significantly less protected from micrococcal nuclease (MNase) digestion up to 6 h postinf

162 erepressed ESs show increased sensitivity to micrococcal nuclease (MNase) digestion, and a decrease i

163 Micrococcal nuclease (MNase) digests the linker to yield

164 Micrococcal nuclease (MNase) is commonly used to map nuc

165 Micrococcal nuclease (MNase) is extensively used in geno

166 ely digested to mononucleosomes using either micrococcal nuclease (MNase) or caspase-activated DNase

167 re-RC) assembly, replication initiation, and micrococcal nuclease (MNase) sensitivity at different ce

168 Micrococcal nuclease (MNase) sequence-based profiles of

169 Digestion of the chromatin with micrococcal nuclease (MNase) shows a nucleosome array wi

170 ome-wide mapping of nucleosomes generated by micrococcal nuclease (MNase) suggests that yeast promote

171 method to measure chromatin accessibility to micrococcal nuclease (MNase) that is normalized for nucl

172 hEC) uses fusion of a protein of interest to micrococcal nuclease (MNase) to target calcium-dependent

173 Like micrococcal nuclease (MNase), MPE-Fe(II) preferentially

174 In this review, we compare the traditional micrococcal nuclease (MNase)-based approach with a chemi

175 Compared to micrococcal nuclease (MNase)-based methods that map nucl

176 bind one or both full nucleosomes that flank micrococcal nuclease (MNase)-defined nucleosome-free pro

177 tes at nucleotide resolution by footprinting micrococcal nuclease (MNase)-sensitive sites.

178 inaccessible to DNA-binding factors, such as micrococcal nuclease (MNase).

179 e extent to which chromatin is digested with micrococcal nuclease (MNase).

180 n these mutants has reduced accessibility to Micrococcal nuclease (MNase).

181 The micrococcal nuclease Nuc1 is one of the major S. aureus

182 chromatic sequences become hypersensitive to micrococcal nuclease, nucleoli fail to form, and transcr

183 Treatment with micrococcal nuclease of highly purified mtRNase P confir

184 products, similar to the well-characterized micrococcal nuclease of Staphylococcus aureus.

185 moderately greater sequence preference than micrococcal nuclease or DNase I, and the sites attacked

186 essed by sedimentation and by digestion with micrococcal nuclease or DNase I.

187 prepared from chromatin digested with either micrococcal nuclease or DNaseI and are restricted in the

188 deed induce sites hypersensitive to DNase I, micrococcal nuclease, or restriction enzymes on either s

189 th Cse4 and H2A are precisely protected from micrococcal nuclease over the entire CDE of all 16 yeast

190 Micrococcal nuclease partial digestion generated a ladde

191 Treatment of the polyribosomes with micrococcal nuclease prior to salt extraction solubilize

192 Use of DNase I and micrococcal nuclease probes further indicated that the s

193 DNase I and micrococcal nuclease probes show that GATA-1 binding cau

194 nt stagger is explained by the finding that micrococcal nuclease produces NCPs not with flush ends,

195 Micrococcal nuclease protection analysis showed that les

196 ich antibody-targeted controlled cleavage by micrococcal nuclease releases specific protein-DNA compl

197 ent protein-tagged H1 variants, we show that micrococcal nuclease-resistant chromatin is specifically

198 ift assay, this protein produced a discrete, micrococcal nuclease-resistant complex with an approxima

199 Characterization of the most micrococcal nuclease-resistant DNA indicates that micron

200 visiae, obtained by paired-end sequencing of micrococcal nuclease-resistant DNA.

201 s that the centromeric nucleosome contains a micrococcal nuclease-resistant kernel of 123-135 bp, dep

202 ementing activity was protease-sensitive and micrococcal nuclease-resistant, had a native molecular m

203 This Asf1p/H3/H4 complex generated micrococcal nuclease--resistant DNA in the absence of DN

204 be produced by pretreatment with DNase I or micrococcal nuclease, respectively.

205 table for analysis of chromatin structure by micrococcal nuclease, restriction endonuclease or by imm

206 ive and inactive CEN chromatin digested with micrococcal nuclease revealed that periodic nucleosome a

207 e of exons 1, 2, and 5 of the DHFR gene with micrococcal nuclease revealed that the exon 1 region is

208 nctional significance of this association, a micrococcal nuclease-sensitive component, i.e., an snRNP

209 PELP1 overexpression increased the micrococcal nuclease sensitivity of estrogen response el

210 he human protamine cluster were subjected to Micrococcal Nuclease-seq.

211 Micrococcal nuclease studies revealed that TRs are embed

212 Consistently, micrococcal nuclease susceptibility analysis revealed al

213 pressed CFS, the FRA3B, is more resistant to micrococcal nuclease than that of the flanking non-fragi

214 Consistently, the accessibility of micrococcal nuclease to chromatin was generally inhibite

215 a cell extracts, which were pre-treated with micrococcal nuclease to degrade the endogenous RNase P R

216 riments where the extracts were treated with micrococcal nuclease to digest endogenous snRNAs, the ef

217 acO/GFP-LacI plants were lysed, treated with micrococcal nuclease to digest the DNA to fragments of a

218 Micrococcal nuclease-treated cell extracts are fully com

219 Using micrococcal nuclease-treated chromatin, we find that Cse

220 ng to successive salt-extracted fractions of micrococcal nuclease-treated Drosophila chromatin.

221 by incubating human 32P-labeled U2 snRNP in micrococcal nuclease-treated HeLa nuclear extracts, foll

222 Addition of DNA to micrococcal nuclease-treated samples restored interactio

223 Micrococcal nuclease-treated TAP preparations were devoi

224 that the 3' ends of capsid pgRNA isolated by micrococcal nuclease treatment are heterogeneously dispe

225 ted by using an established method involving micrococcal nuclease treatment demonstrated reduced leve

226 as not bridged by nucleic acids, as shown by micrococcal nuclease treatment of the proteins prior to

227 A (vRNA) has been depleted by treatment with micrococcal nuclease, was used to study transcription in

228 By using micrococcal nuclease, which has both endo- and exo-nucle

229 eosome cores were liberated using an enzyme (micrococcal nuclease) with a strong preference for cleav