ライフサイエンスコーパス: microcystin

1 notoxins often rely on the quantification of microcystin.

2 bability of a beach sample exceeding 4 mug/L microcystin.

3 de of 12-18 product ions for each identified microcystin.

4 on sites was unaffected by MgCl(2), EDTA, or microcystin.

5 hatase that is inhibited by okadaic acid and microcystin.

6 more linear with time and was unaffected by microcystin.

7 t affected by the intracellular perfusion of microcystin.

8 rine/threonine protein phosphatase inhibitor microcystin.

9 actions induced by the phosphatase inhibitor microcystin.

10 urified with A and C subunits on immobilized microcystin.

11 phorylation was increased by the addition of microcystin.

12 to identify peaks corresponding to candidate microcystins.

13 thiol to the alpha,beta-unsaturated amide of microcystins.

14 ens from all over the world commonly produce microcystins.

15 ides such as lantibiotics, thiopeptides, and microcystins.

16 )R (5) and MC-M(O)R (7), as well as 20 other microcystins.

17 echanism, since the effect was reproduced by microcystin (10 microM in pipette solution), which is a

18 while the non-specific phosphatase inhibitor microcystin (250 nM) increased channel activity by 218%.

19 ed method for measuring the concentration of microcystin, a group of toxins associated with cyanobact

20 The transport of microcystin, a hepatotoxin produced by cyanobacteria (e.

21 Intracellular application of microcystin, a membrane-impermeable inhibitor of phospha

22 Intracellular perfusion with microcystin, a potent phosphatase 1 and 2a inhibitor, in

23 ts in pull-down experiments with immobilized Microcystin, a PP2A inhibitor.

24 ones, intracellular application of 20 microM microcystin, a protein phosphatase 1/2A inhibitor, prolo

25 Nuclear extracts were subjected to microcystin affinity chromatography to recover phosphata

26 by Mono Q anion exchange chromatography and microcystin affinity chromatography.

27 R was verified by co-immunoprecipitation and microcystin affinity purification.

28 Microcystin-affinity chromatography was used to purify 1

29 Third, microcystin-agarose depletes mitotic extracts of both PP

30 Microcystin-agarose pull-downs suggested that a phosphat

31 phosphatase-specific activity or binding to microcystin-agarose.

32 onine phosphatase inhibitors calyculin A and microcystin also stimulated SOCs in isolated outside-out

33 effects of EET persisted in the presence of microcystin, an inhibitor of protein phosphatases 1 and

34 This microcystin analog and potent hepatotoxin has previously

35 s approach simplifies detection of candidate microcystin analogues even in the presence of complex mi

36 More than 150 microcystin analogues have been reported from cultures,

37 Because the effects of microcystin and EDTA were additive, and microcystin did

38 New information was obtained using (a) microcystin and okadaic acid to inhibit serine/threonine

39 PKA (C subunit) and phosphatase inhibitors (microcystin and okadaic acid) had no effect on forskolin

40 Binding of PP2Ac to microcystin and to alpha-4 increased with temperature, c

41 to the toxins calyculin A, okadaic acid, and microcystin and to thiophospho-DARPP-32.

42 cate that the ELISA has broad specificity to microcystins and also detects nodularin, providing a sen

43 tides, including the widespread hepatotoxins microcystins and nodularins.

44 protein phosphatase inhibitors okadaic acid, microcystin, and protein phosphatase inhibitor-2.

45 ed LC-MS approach to identify Dhb-containing microcystins, and allowed identification of LC-MS peaks

46 Cyclosporin, microcystins, and nodularins are all notable pharmacolog

47 Microcystins are a group of cyclic heptapeptides origina

48 Microcystins are a major group of cyanobacterial heptape

49 Microcystins are cyclic heptapeptides produced by a rang

50 Microcystins are potent toxins that are responsible for

51 One suggestion is that microcystins are siderophores (i.e., ligands with an ext

52 icrocystin-leucine-tryptophan (MC-LW) 63.7%, microcystin-arginine-arginine (MC-RR) 60.1% and nodulari

53 CLR (microcystin-leucine-arginine) and MCRR (microcystin-arginine-arginine) at a sublethal dose (10 m

54 ents' serum, and postmortem liver tissue for microcystin assays.

55 the first known report of a recombinant anti-microcystin avian antibody.

56 rential effects of the two nucleotides since microcystin, beta-glycerol phosphate, or okadaic acid co

57 Polymeric microcystin binding iron may be related with a toxic cel

58 DS-polyacrylamide gel electrophoresis of the microcystin-biotin eluates of the three fractions reveal

59 harose by a rebinding interaction with PP-1C-microcystin-biotin.

60 imulated the dephosphorylation of NPR-A, and microcystin blocked the temperature-dependent dephosphor

61 Protein sequencing revealed that the microcystin-bound proteins with the greatest reduction i

62 nine phosphatase inhibitors okadaic acid and microcystin, but is inhibited by the tyrosine phosphatas

63 en they enter directly into the circulation, microcystins can lead to fatal clinical syndromes rangin

64 In 48 (26.4%) samples we observed microcystin concentrations as measured by ELISA that exc

65 Microcystin concentrations decline by approximately 98%

66 hat addresses these issues and that predicts microcystin concentrations from summer mean total nitrog

67 del accounts for 69% of the variance in mean microcystin concentrations in lakes and reservoirs of th

68 more robust and useful metric for predicting microcystin concentrations than qPCR measurements enumer

69 Mean microcystin concentrations were more strongly associated

70 enrichment yielding significant increases in microcystin concentrations.

71 agment displayed cross-reactivity with seven microcystin congeners (microcystin-leucine-arginine (MC-

72 RR and a range of other [Mdha(7)]-containing microcystin congeners.

73 also to minimize specificity for particular microcystin congeners.

74 rocedure that allows LC-MS identification of microcystins containing methionine and methionine sulfox

75 This suggests that microcystins containing methionine sulfoxide are primari

76 C-MS analysis, clearly discriminated between microcystins containing the isobaric [Dhb(7)]- and [Mdha

77 as sufficiently large that derivatization of microcystin-containing samples with mercaptoethanol, fol

78 (6), which comprised about half of the total microcystin content in the bloom, and ferintoic acids C

79 et under low N and a significant decrease in microcystin content per Microcystis cell demonstrating t

80 The effect of microcystin could be blocked by co-applying PKA inhibito

81 s, the calculated photochemical half-life of microcystin decreased 6-fold with increasing salinity al

82 The efficiency of current microcystin detection methods has been hampered by the l

83 e to achieve sensitive and congener-specific microcystin detection with detection limit as low as 10

84 targeting molecules and their application in microcystin detection.

85 s of microcystin and EDTA were additive, and microcystin did not block the magnesium-dependent desens

86 Biotinylated microcystin displayed IC50 values in the nM range agains

87 imulated the dephosphorylation of NPR-A, and microcystin failed to inhibit this process.

88 ediated photodegradation in future models of microcystin fate in freshwater-estuarine systems.

89 A competitive assay with microcystin-horseradish peroxidase conjugate was optimiz

90 ICl,CaMK by okadaic acid (IC50 = 1.5 nM) and microcystin (IC50 = 0.15 nM); these data lead to the nov

91 Inside cells, with microcystin implicated in the fitness of the photosynthe

92 Contractions induced by microcystin in Ca2+-free solution were associated with i

93 The limit of quantitation for microcystins in drinking water is 0.04 mug/L, well below

94 to monitor cyanobacterial blooms and detect microcystins in freshwater bodies.

95 evealed the presence of only trace levels of microcystins in the edible parts.

96 The ELISA can be used for quantifying total microcystins in various matrices, including drinking wat

97 eawater halides increased quantum yields for microcystin indirect photodegradation by factors of 3-6.

98 The observed sensitivity of the microcystin-induced contraction to various protein kinas

99 the phosphatase inhibitors okadaic acid and microcystin inhibit transport mediated by the import rec

100 ine/threonine protein phosphatase inhibitor, microcystin, inhibited the desensitization of NPR-A in m

101 detectable cross-reactivity to okadaic acid, microcystin-LA, and microcystin-YR.

102 microcystin-tyrosine-arginine (MC-YR) 79.7%, microcystin-leucine-alanine (MC-LA) 74.8%, microcystin-l

103 reactivity with seven microcystin congeners (microcystin-leucine-arginine (MC-LR) 100%, microcystin-t

104 The sensing surface consisted of microcystin-leucine-arginine (MCLR) covalently immobiliz

105 neation exposure of adult zebrafish to MCLR (microcystin-leucine-arginine) and MCRR (microcystin-argi

106 , microcystin-leucine-alanine (MC-LA) 74.8%, microcystin-leucine-phenylalanine (MC-LF) 67.5%, microcy

107 ocystin-leucine-phenylalanine (MC-LF) 67.5%, microcystin-leucine-tryptophan (MC-LW) 63.7%, microcysti

108 bed is the total synthesis of the cyanotoxin microcystin-LF (MC-LF, 1a) and two derivatives thereof.

109 Microcystin LR (MCLR) was covalently bound to the dextra

110 ition of PP2A activity by okadaic acid (OA), microcystin LR (mLR), or fostriecin (Fos) leads to perik

111 Detection limits of 0.4 mug/L for microcystin LR and 10(0) and 10(1) cfu/mL for Salmonella

112 brary against the cyanobacterial hepatotoxin microcystin LR and its selection using competitive panni

113 phatases (sodium fluoride, okadaic acid, and microcystin LR).

114 A protein phosphatase inhibitor, microcystin LR, also induced contraction in the absence

115 igate unexplored molecular pathways by which microcystin-LR (MC-LR) acts on hepatocytes to elucidate

116 A novel electrochemical microcystin-LR (MC-LR) biosensor based on the inhibition

117 ined selective capture and SERS detection of Microcystin-LR (MC-LR) in blood plasma has been develope

118 waveguide immunosensor for the detection of microcystin-LR (MC-LR) in lake water.

119 264.7 macrophages, we showed the potency of microcystin-LR (MC-LR) to stimulate production of pro-in

120 The presence of the potent cyanotoxin, microcystin-LR (MC-LR), in drinking water sources poses

121 xpressing a recombinant antibody specific to microcystin-LR (MC-LR), the environmental toxin pollutan

122 ork, we explore proton and iron binding with microcystin-LR (MC-LR).

123 The compounds chosen for this study were microcystin-LR (MLR), phenobarbital (PB), lipopolysaccha

124 rd other coexistent cyanobacterial toxins of microcystin-LR and Anatoxin-a.

125 ects on okadaic acid and nodularin, and with microcystin-LR and inhibitor-2 being the least affected.

126 hly sensitive to three toxins (okadaic acid, microcystin-LR and tautomycin), which block PP1- and PP2

127 To immobilize microcystin-LR antibody and improve the electrical condu

128 dropped on the electrode surface and finally microcystin-LR antibody was covalently connected to the

129 Microcystin-LR binds also Mo(6+), Cu(2+), and Mn(2+).

130 The phosphatase inhibitor microcystin-LR blocked rescue of microtubule sliding, in

131 r and before incubation with 2.0x10(-15)M of microcystin-LR can retain 95% over a 20-weeks storage pe

132 inked immunosorbent assay (ELISA) format for microcystin-LR detection was developed, achieving a dete

133 ptamer assembled on a graphene electrode for microcystin-LR detection was developed.

134 It has been successfully applied to the microcystin-LR determination in water samples with a spi

135 or the presence of the active site inhibitor microcystin-LR did not interfere with binding of PP2Ac t

136 Using atomic force microscopy, 40% of microcystin-LR dimers were observed, and the presence of

137 meaningful environmental pHs values shows a microcystin-LR dissociaton constant for Fe(2+) and Fe(3+

138 protein kinase, but did require inclusion of microcystin-LR during cell lysis, implying that phosphor

139 ses on the immobilized phosphatase inhibitor microcystin-LR identified histone deacetylase 1(HDAC1),

140 effective, and suitable for the detection of microcystin-LR in buffer and spiked tap and river water

141 m would facilitate the routine monitoring of microcystin-LR in real samples.

142 1.2%), sensitive electrochemical response to microcystin-LR in the range of 1.0x10(-16)-8.0x10(-15)M

143 immunosensor for ultrasensitive detection of microcystin-LR in water.

144 ymer (MIP) specific for Cyanobacterial toxin microcystin-LR is presented.

145 ility and stability when compared to present microcystin-LR sensors.

146 peak current, allowing the quantification of microcystin-LR through the measurement of peak current c

147 binding was eliminated by addition of excess microcystin-LR to the lysate, showing that binding at th

148 lyclonal antibodies (the detection limit for microcystin-LR using the MIP-based assay was found to be

149 A detection limit of 0.5 mug L(-1) for microcystin-LR was achieved.

150 novel immuno-sensing format can detect free microcystin-LR with a functional limit of detection of 0

151 sor also exhibited excellent selectivity for microcystin-LR with no detectable cross-reactivity to ok

152 hat exhibit high affinity and specificity to microcystin-LR, -YR, and -LA.

153 reated simultaneously with cycloheximide and microcystin-LR, a potent in vivo and in vitro inhibitor

154 Microcystin-LR, a PP1 inhibitor, also attenuated I-2 bin

155 We found that okadaic acid, microcystin-LR, and calyculin A, which are known to spec

156 bstrates and were resistant to okadaic acid, microcystin-LR, and trypsin.

157 , the tumor-inducing agents okadaic acid and microcystin-LR, at 2.6 and 2.8 A resolution, respectivel

158 anobacterially produced cyclic heptapeptide, microcystin-LR, both potent inhibitors of mammalian PP1

159 50 with active site inhibitors okadaic acid, microcystin-LR, calyculin A, and cantharidin.

160 s insensitive to inhibition by okadaic acid, microcystin-LR, calyculin A, and cantharidin.

161 1, and several toxins, including tautomycin, microcystin-LR, calyculin A, and okadaic acid.

162 binding site for the toxins okadaic acid and microcystin-LR, in the beta12-beta13 loop with Trp, Phe,

163 The cyanobacteria toxins anatoxin-a, microcystin-LR, microcystin-RR, microcystin-YR, and nodu

164 -type and mutant phosphatases to immobilized microcystin-LR, NIPP-1, and I-2 established that the bet

165 oward okadaic acid, tautomycin, calyculin A, microcystin-LR, nodularin, inhibitor-2, and cantharidic

166 tly inhibit eucaryal PP1 and PP2A, including microcystin-LR, okadaic acid, tautomycin, and calyculin

167 The presence of microcystin-LR, on the other hand, caused a dose-respons

168 he visible light-driven rapid degradation of microcystin-LR, one of the most toxic compounds produced

169 ications to produce diagnostic antibodies to microcystin-LR, remove it from the environment by phytor

170 eabilized rabbit portal vein contracted with microcystin-LR, the ability of the following fragments o

171 In support of this concept, okadaic acid and microcystin-LR, which are inhibitors of protein phosphat

172 PP4) belongs to a family of okadaic acid and microcystin-LR-sensitive protein phosphatases.

173 or the detection of the cyanobacterial toxin microcystin-LR.

174 nt to hepatoxicity induced by phalloidin and microcystin-LR.

175 ctivated the Nek2::PP1 to the same extent as microcystin-LR.

176 ealing study was used in MIP preparation for microcystin-LR.

177 hy on the immobilized phosphatase inhibitor, microcystin-LR.

178 ffered by previously reported biosensors for microcystin-LR.

179 from a cyanobacterium that does not produce microcystins, M. aeruginosa UTEX 2063.

180 Although there have been numerous studies on microcystin (MC) accumulation in aquatic organisms recen

181 Our results showed that bloom growth and microcystin (MC) concentrations responded more frequentl

182 tudy examines the effects of electrolytes on microcystin (MC) electrospray ionization (ESI) mass spec

183 protein phosphatase 1 and 2A inhibitors like microcystin (MC) or okadaic acid (OA).

184 d what environmental parameters may regulate microcystin (MC) production and congener type.

185 Microcystin (MC), a cyanotoxin, is the most widely repor

186 The cyanotoxin, microcystin (MC), is known to accumulate in the tissues

187 and this was blocked by a PP1/PP2A inhibitor microcystin (MC)-LR or by mutation of the active sites i

188 The known microcystins (MC) MC-LR, MC-LA, [MeSer7]MC-LR, MC-LL, MC

189 ctive compounds including the harmful toxins microcystins (MC).

190 and analogues), cylindrospermopsin (CYN) and microcystins (MC, and analogues).

191 eric noncompetitive assay for cyanobacterial microcystins (MCs) and nodularins (Nod), a group of stru

192 Microcystins (MCs) are a group of biotoxins (>150) produ

193 Microcystins (MCs) are a group of hepatotoxins produced

194 Microcystins (MCs) are a growing problem in drinking wat

195 Microcystins (MCs) are hepatotoxins produced by cyanobac

196 Microcystins (MCs) are highly toxic natural products whi

197 Microcystins (MCs) are primarily hepatotoxins produced b

198 tion has been developed for the detection of microcystins (MCs) in freshwater samples.

199 To evaluate ecotoxicological effect of microcystins (MCs) on earthworms, filter paper acute tox

200 yanobacteria producing hepatotoxins, such as microcystins (MCs), together with other bioactive compou

201 of Planktothrix rubescens, which can produce microcystins (MCs), was observed in early 2009 in the Oc

202 iorecognition molecules for the detection of microcystins (MCs).

203 serine-threonine protein phosphatases (PP), microcystin (MCYST) and okadaic acid (OKA) as probes to

204 scovery of three new cyclic peptides, namely microcystin-MhtyR (6), which comprised about half of the

205 ining raw test data from regularly scheduled microcystin monitoring program or (2) the manufacturer o

206 Here, we explored the effect of microcystin/nonmicrocystin (MC/non-MC) producing cyanoba

207 Compounds (calyculin A, microcystin, okadaic acid, and cantharidin) that inhibit

208 und either the biosynthetic genes for making microcystins or the toxin itself in 12% of all analyzed

209 -1C by inhibitor-2, but not by okadaic acid, microcystin, or calyculin A, was also attentuated by the

210 thiophosphorylation at Ser(19), followed by microcystin (phosphatase inhibitor) in the absence of Ca

211 Modeling of microcystin photodegradation along this transect indicat

212 ies (RHS)) could significantly contribute to microcystin photodegradation during transport within est

213 dicated that the time scale for RHS-mediated microcystin photodegradation is comparable to the time s

214 nobacterial cells, and the immobilization of microcystins, preventing their release into the water co

215 Microcystins produced by cyanobacteria were detected in

216 rotein-serine/threonine phosphatase from the microcystin-producing cyanobacterium Microcystis aerugin

217 The microcystin-producing Microcystis aeruginosa PCC 7806 an

218 trains of Microcystis, including 6 different microcystin-producing strains.

219 encoding the enzyme complex responsible for microcystin production and detecting toxins directly fro

220 t phenylephrine-induced contractions but not microcystin-racemic mixture-induced contractions.

221 ay disc and applied for the determination of microcystin residues and pathogenic microorganisms.

222 simulation data suggest a wind event caused microcystin-rich water from Maumee Bay to be transported

223 challenges, a numerical index for screening microcystin risks above the World Health Organization's

224 oss-reactivity to other related MCs, such as microcystin-RR (MCRR, 90%), microcystin-RR desmethylated

225 ted MCs, such as microcystin-RR (MCRR, 90%), microcystin-RR desmethylated (dm-MCRR, 95%) and microcys

226 obacteria toxins anatoxin-a, microcystin-LR, microcystin-RR, microcystin-YR, and nodularin were separ

227 nously applied protein phosphatase 1 or by a microcystin-sensitive phosphatase also endogenous to exc

228 fied with PP1c by affinity chromatography on microcystin sepharos Immunocytochemical analysis demonst

229 PP2A; and 3) PAK3 and p70 S6 kinase bound to microcystin-Sepharose (an affinity resin for PP2A-PP1).

230 covered from the isothiocyanate eluates from microcystin-Sepharose by a rebinding interaction with PP

231 rotein, termed PP4R1, and PP4C also bound to microcystin-Sepharose.

232 fusion of the antigen-binding regions of the microcystin-specific single-chain antibody, 3A8, with co

233 ition of protein phosphatases with 10 microM microcystin stimulated both ICa and ICl, but the stimula

234 n-replete conditions, while the nonproducing microcystin strain is not able to grow.

235 the mcyE gene and the chemical diversity of microcystins suggest that lichen symbioses may have been

236 ular dialysis with the phosphatase inhibitor microcystin, suggesting involvement of endogenous phosph

237 nobacterium and the role of N in controlling microcystin synthesis.

238 time quantitative PCR (qPCR) measurements of microcystin synthetase E (mcyE) gene equivalents (Adj. R

239 ificant decrease in the transcription of the microcystin synthetase gene set under low N and a signif

240 tease inhibitors (aer and mcn gene sets) and microcystin synthetase genes (mcy), with urea enrichment

241 Here, we report the development of novel microcystin-targeting molecules and their application in

242 ocess that gives valuable sequence ions from microcystins that do not contain arginine.

243 lters, and water-treatment columns contained microcystins, the highly toxic low-molecular-weight hepa

244 measured by ELISA that exceeded the 4 mug/L microcystin threshold.

245 gger changes (for example, in the binding of microcystin to proteins).

246 n immunogen made by conjugating a mixture of microcystins to cationised bovine serum albumin, and the

247 xicity estimation in terms of cyanobacterial microcystin toxins (MCs) detection.

248 The cyclic heptapeptide microcystin toxins produced by a strain of Microcystis a

249 (microcystin-leucine-arginine (MC-LR) 100%, microcystin-tyrosine-arginine (MC-YR) 79.7%, microcystin

250 0.83, p < 0.0001) was equally predicative of microcystin variance across the lake as fluorescence bas

251 dominated by Microcystis sp. and associated microcystin variants, have been implicated in illnesses

252 Organization's (WHO) low-risk threshold for microcystin was developed for eutrophic Midwestern U.S.

253 The effect of microcystin was inhibited by staurosporine (Ki = 171.5 a

254 Biotinylated microcystin was used to affinity purify over avidin-Seph

255 A plethora of different microcystins was found with over 50 chemical variants, a

256 alpha,beta-unsaturated amide present in most microcystins was shown to simplify analysis of LC-MS chr

257 ark (2.9 x 10(1) mug/L), where low levels of microcystin were found (2.1 x 10 degrees mug/L).

258 The microcystins were separated by reversed-phase microbore

259 d and very reliable identifications of known microcystins when standards are not available and of mos

260 members of a few genera produce hepatotoxic microcystins, whereas production of hepatotoxic nodulari

261 ng mice to the hepatotoxins, griseofulvin or microcystin, which are associated with K18 ser52 and oth

262 t blocking was inhibited by okadaic acid and microcystin with IC50 values of 70 nM and 0.15 nM, respe

263 rocystin-RR desmethylated (dm-MCRR, 95%) and microcystin-YR (MCYR, 91%), was also evaluated.

264 es that nodularin was preferred over [15N]10-microcystin-YR or angiotensin I.

265 anatoxin-a, microcystin-LR, microcystin-RR, microcystin-YR, and nodularin were separated in less tha

266 ctivity to okadaic acid, microcystin-LA, and microcystin-YR.