ライフサイエンスコーパス: microhomology

1 ed junctions or junctions with insertions or microhomology).

2 curate non-processive DNA polymerase creates microhomology.

3 quently correlates with increased junctional microhomology.

4 s, deletions, duplications, and instances of microhomology.

5 cal NHEJ and brings about repair at sites of microhomology.

6 joining that was mediated by 2-, 3- or 10-bp microhomology.

7 e occurred at regions of naturally occurring microhomology.

8 ted number of nucleotides back to regions of microhomology.

9 s often stabilized by up to 4 bp of terminal microhomology.

10 olving joining between regions of nucleotide microhomology.

11 ells occurred at unusually long stretches of microhomology.

12 , thus stabilizing the junction at a site of microhomology.

13 earing 5-, 8-, 10-, 13-, 16-, 19-, and 22-nt microhomology.

14 involving template switching at positions of microhomology.

15 tions were formed via end-joining with short microhomologies.

16 ditions, and 5' boundary and inversion point microhomologies.

17 ination reactions can be detected using such microhomologies.

18 tions may be associated with the presence of microhomologies.

19 Alternative NHEJ revealed 2 to 5 bp microhomology (15.7% of cases) or new replication-mediat

20 ases, three involving DMD and one HEXB gene, microhomologies (2-10 bp) were observed at breakpoint ju

21 engths and the annealing of short regions of microhomology (2-6 bp) across the break-site.

22 s (19.2%) and substantially less reliance on microhomology (31%) than previously observed in benign c

23 cleavage site indicative of DSB repair using microhomology (6-12 bp perfect repeats, or 10-16 bp with

24 another viral fragment by a short homology (microhomology), a hallmark of illegitimate recombination

25 er, we did observe greater usage of terminal microhomology among NHEJ events recovered from wild-type

26 The pattern of microhomology among switch junction sites in Msh2-defici

27 altered in these cells, with unusually long microhomologies and a lack of direct end-joining.

28 Most Ds and fAc deletion junctions displayed microhomologies and contained filler DNA from nearby seq

29 aims to solve the problem of deletions with microhomologies and deletions with microinsertions.

30 f deletions: blunt deletions, deletions with microhomologies and deletions with microsinsertions.

31 it accounts for most repairs associated with microhomologies and is made efficient by coupling a micr

32 ctions with apparent blunt joins, junctional microhomologies and short indels (deletion with insertio

33 epairs DNA breaks via the use of substantial microhomology and always results in deletions.

34 vative NHEJ (C-NHEJ), which does not require microhomology and can join ends precisely; and deletiona

35 m sequencing data, especially deletions with microhomology and deletions with microinsertion.

36 somatic breakpoints show significantly less microhomology and fewer templated insertions than germli

37 recombination junctions at ectopic sites of microhomology and implicated nucleolytic degradation in

38 The co-occurrence of microhomology and inserted sequence is low (10%), sugges

39 reaks is mediated by annealing at regions of microhomology and is always associated with deletions at

40 ons in tert ku70 lig4 mutants contained less microhomology and less telomeric DNA.

41 panzee numt integrations were accompanied by microhomology and short indels of the kind typically obs

42 o main classes: those with short homologies (microhomologies) and those with inserted DNA of uncertai

43 DNA motif; however, deletions, duplications, microhomologies, and nontemplate DNA were found.

44 involving at least two consecutive rounds of microhomology annealing and synthesis across the break s

45 ever, alt-EJ also produces junctions without microhomology (apparent blunt joins), and the exact role

46 use of an alternative joining pathway where microhomologies are important for CSR break ligation.

47 forms, triplexes and tetraplexes) as well as microhomologies are postulated to participate in the rec

48 With homeologous donors, ICTS uses microhomologies as small as 2 bp.

49 or Exo1 result in increased switch junction microhomology as has also been seen with Msh2 deficiency

50 und deficiency of IgG3 in most mice and long microhomologies at Ig switch (S) joints.

51 The structures of the mutants revealed microhomologies at the breakpoints, consistent with a no

52 igns of nonhomologous end joining, including microhomologies at the end points, and small deletions a

53 mall templated insertions at breakpoints and microhomology at breakpoint junctions, which have been a

54 l types of nonhomologous-end-joining joints: microhomology at junctions (57%), insertion of sequences

55 determining regions 3 and long stretches of microhomology at switch junctions.

56 tely 3 Mb) size distribution and overlapping microhomology at the breakpoints.

57 formed coding joints, but also the extent of microhomology at the coding junctions.

58 rt mutants, there was a greater incidence of microhomology at the fusion junction than in tert mutant

59 chromosome fusions were still detected, but microhomology at the junction was no longer favored.

60 nome-wide, sized 1-100 base pairs often with microhomology at the junction.

61 at CSR sites indicated that there is greater microhomology at the mu-gamma1 switch junctions in ATM B

62 well-defined NHEJ pathway, characterized by microhomology at the repair junctions, play a role in th

63 in Xrcc1(+/-) splenic B cells, the length of microhomology at the switch junctions decreased, suggest

64 ions of DNA sequence homology, also known as microhomology, at chromosomal breakpoints.

65 evealed a variety of interactions, including microhomology base pairing, mismatched and flipped-out b

66 nd a shift toward the use of an alternative, microhomology-based end joining during CSR.

67 sults in impaired CSR and increased usage of microhomology-based end joining.

68 This recombination event employs a microhomology-based end-joining repair pathway, as oppos

69 budding yeast and has been implicated in the microhomology-based joining.

70 A shift to the use of microhomology-based, alternative end-joining (A-EJ) and

71 -deficient cells showed decreased lengths of microhomology between Smicro and Sgamma3 relative to jun

72 eficient B cells showed decreased lengths of microhomology between Smu and Sgamma3 relative to wild-t

73 akpoint junctions are characterized by short microhomologies, blunt ends, and short insertions.

74 rrangement and increased usage of junctional microhomologies both of which also converted to the adul

75 d neither frequency nor length of junctional microhomology, but significantly increased insertion fre

76 lution of DNA breaks with low or no terminal microhomology by a classical nonhomologous end-joining m

77 es do not exist, we have postulated that new microhomologies can be created via limited DNA synthesis

78 rotein/MSH2-dependent pathway that relies on microhomology can act concurrently but independently to

79 bset of ends that thereby gain some terminal microhomology can then be ligated.

80 quences were found on the plasmid, joined by microhomologies characteristic of nonhomologous end-join

81 mologous end joining (NHEJ) that can involve microhomologies close to the broken ends.

82 of these breakpoint junctions had 0-4 bp of microhomology consistent with chromothripsis, and both d

83 cation junctions produced contain regions of microhomology consistent with operation of the nonhomolo

84 unctions were characterized by short (<6 bp) microhomologies, consistent with the hypothesis that the

85 results in DNA ligation at distant sites of microhomology, creating large DNA deletions.

86 etermined both breakpoints flanked by a 4-bp microhomology (CTTG).

87 cing of vector-chromosome junctions detected microhomologies, deletions and insertions that were simi

88 d in ku70 or lig4, DNA repair was shifted to microhomology-dependent alternative NHEJ.

89 of >2 kb deletions and in the usage of long microhomologies distal to the break site, compared with

90 When accessible pre-existing microhomologies do not exist, we have postulated that ne

91 further indicate that a process identical to microhomology-driven single-strand annealing resolves L1

92 of slippage and strand switching at sites of microhomology during replication.

93 ests a role for the BLM helicase in aligning microhomology elements during recombinational events in

94 loom's syndrome (BS) cells are unable to use microhomology elements within the supF20 gene to restore

95 ors, in which non-homologous end joining and microhomology end joining are the predominant mechanisms

96 in both the flip and flop orientations, with microhomology evident at the junctions.

97 Microhomology existed at most junctions between φX17

98 Reducing the microhomology extent between long overhangs reduced thei

99 are aligned using short regions of sequence microhomology followed by processing of the aligned DNA

100 Furthermore, the ability to use microhomologies for end joining was compromised in senes

101 s cerevisiae cells to analyze DSBs requiring microhomologies for repair, known as microhomology-media

102 polymerases use the overhangs as regions of microhomology for template synapsis.

103 servative pathway involving the annealing of microhomologies found within the 17-nt overhangs produce

104 ic recombinants with two distinct patches of microhomology, implying that these proteins are crucial

105 preference for switch junctions with longer microhomologies in Mlh1(GR/GR) mice suggests that throug

106 revious reports showing decreased S-junction microhomologies in MSH2-deficient mice and an exonucleas

107 y end-joining pathway relies more heavily on microhomologies in producing deletions.

108 intra-Smu region recombinations, no/minimal microhomologies in S-S junctions, decreased c-Myc/IgH tr

109 lternative(A)-NHEJ pathway, which introduces microhomologies in S-S junctions.

110 ced more insertions and fewer donor/acceptor microhomologies in Smu-Sgamma1 and Smu-Sgamma3 DNA junct

111 apparent blunt joins), and the exact role of microhomology in both alt-EJ and classical non-homologou

112 however, preference may be due to the use of microhomology in the V, D, and J segments.

113 In this study, we have therefore shown that microhomology in this area of chromosome 1 predisposes t

114 had significantly longer deletions and more microhomology, indicative of alt-NHEJ.

115 d MSH5 alleles show increased donor/acceptor microhomology, involving pentameric DNA repeat sequences

116 Hence, annealing at sites of microhomology is very important, but the flexibility of

117 CSR joins also display direct and microhomology joins, and CSR has been suggested to use C

118 ng pathway, which is markedly biased towards microhomology joins, supports CSR at unexpectedly robust

119 ds with several base-pair homologies to form microhomology joins.

120 ivergence and exhibit a different pattern of microhomology junctions.

121 delta) is critical for MMEJ, independent of microhomology length and base-pairing continuity.

122 repair efficiency increased concomitant with microhomology length and decreased upon introduction of

123 Break proximity, microhomology length and GC-content all favored repair a

124 ions and that PARP proteins were involved in microhomology mediated end joining (MMEJ), one of the ch

125 earrangements, which predominantly reflected microhomology mediated illegitimate recombination involv

126 was predominantly, and possibly exclusively, microhomology mediated, a situation unique among organis

127 Ku and favor repair by the Lig4-independent microhomology-mediated A-EJ process.

128 t resected DNA is preferentially repaired by microhomology-mediated A-NHEJ.

129 eas classical NHEJ (C-NHEJ) is undetectable, microhomology-mediated alternative NHEJ efficiently repa

130 he shelterin-free telomeres are processed by microhomology-mediated alternative-NHEJ when Ku70/80 is

131 opsis chloroplast that resemble the nuclear, microhomology-mediated and nonhomologous end joining pat

132 Models such as microhomology-mediated break-induced replication (MM-BIR

133 stalling and template switching (FoSTeS) and microhomology-mediated break-induced replication (MMBIR)

134 microhomology, suggesting replication error Microhomology-Mediated Break-Induced Replication (MMBIR)

135 echanistic details have been provided in the microhomology-mediated break-induced replication (MMBIR)

136 Recently, CGRs were suggested to result from microhomology-mediated break-induced replication (MMBIR)

137 talling and template switching (FoSTeS), and microhomology-mediated break-induced replication (MMBIR)

138 most often via nonhomologous end joining or microhomology-mediated break-induced replication.

139 her diseases have revealed the occurrence of microhomology-mediated chromosome rearrangements and cop

140 utes to two error-prone DSB repair pathways: microhomology-mediated end joining (a Ku86-independent m

141 Microhomology-mediated end joining (MHEJ) is always acco

142 showed that DNA ends can also be joined via microhomology-mediated end joining (MHEJ), especially wh

143 Microhomology-mediated end joining (MMEJ) is a major pat

144 This microhomology-mediated end joining (MMEJ) is Ku independ

145 Microhomology-mediated end joining (MMEJ) joins DNA ends

146 Of interest, robust microhomology-mediated end joining (MMEJ) was observed w

147 Microhomology-mediated end joining (MMEJ), an error-pron

148 wever, a mutagenic alternative NHEJ pathway, microhomology-mediated end joining (MMEJ), can also be d

149 , less characterized repair mechanism, named microhomology-mediated end joining (MMEJ), has received

150 minated by homologous recombination (HR) and microhomology-mediated end joining (MMEJ), while non-hom

151 an alternative error-prone mechanism termed microhomology-mediated end joining (MMEJ).

152 s, we propose a model of synthesis-dependent microhomology-mediated end joining (SD-MMEJ), in which d

153 This model, called synthesis-dependent microhomology-mediated end joining (SD-MMEJ), predicts t

154 evidence that Brca1 has an essential role in microhomology-mediated end joining and suggest a novel m

155 hich sister chromatid fusion is initiated by microhomology-mediated end joining of double strand brea

156 a de novo 6.3-kb deletion that arose through microhomology-mediated end joining rather than nonalleli

157 family where a deletion has occurred through microhomology-mediated end joining rather than nonalleli

158 ce and an exonuclease 1 (EXO1) role in yeast microhomology-mediated end joining suggest that mismatch

159 e strand breaks by homologous recombination, microhomology-mediated end joining, and single strand an

160 ously unrecognized complex events, involving microhomology-mediated end joining, preceded or accompan

161 -Cas self targeting indicated DNA repair via microhomology-mediated end joining.

162 n origin by DNA repair synthesis followed by microhomology-mediated end joining.

163 o HR, CtIP dimerization is also required for microhomology-mediated end joining.

164 Here we show that a null mutation in the microhomology-mediated end-joining (MMEJ) component, pol

165 trand breaks, referred to as the error-prone microhomology-mediated end-joining (MMEJ) pathway.

166 of 53BP1 are correlated with a promotion of microhomology-mediated end-joining (MMEJ), a subtype of

167 (HR), nonhomologous end-joining (NHEJ), and microhomology-mediated end-joining (MMEJ).

168 occurs through non-homologous end-joining or microhomology-mediated end-joining (MMEJ).

169 quiring microhomologies for repair, known as microhomology-mediated end-joining (MMEJ).

170 ) MEFs exhibited a 50-100-fold deficiency in microhomology-mediated end-joining activity of a defined

171 As microhomology-mediated end-joining requires annealing of

172 nation in Aplf(-/-) B cells is biased toward microhomology-mediated end-joining, a pathway that opera

173 nd propose that class switching can occur by microhomology-mediated end-joining.

174 man breast carcinoma cells while suppressing microhomology-mediated error-prone end-joining and restr

175 cribe the features and mechanistic models of microhomology-mediated events, discuss their physiologic

176 ats, whereas the other two fusions exhibited microhomology-mediated events.

177 and AGCT) at the breakpoints indicated that microhomology-mediated FoSTeS events were involved in th

178 stranded DNA binding protein (RFA1) increase microhomology-mediated GCR formation.

179 athways can suppress GCRs: two that suppress microhomology-mediated GCRs (RFA1 and RAD27) and one tha

180 at double-strand breaks induce a genome-wide microhomology-mediated illegitimate recombination pathwa

181 I-SceI-mediated double-strand break induces microhomology-mediated integration randomly throughout t

182 Irradiated yeast cells displayed 77% microhomology-mediated integration, compared to 27% in u

183 estriction enzymes increase the frequency of microhomology-mediated integration.

184 Microhomology-mediated joining appears to serve a subsid

185 s during CSR, supporting a role for XRCC1 in microhomology-mediated joining.

186 Microhomology-mediated linking of disparate segments of

187 rom compatible termini, an essential step in microhomology-mediated NHEJ.

188 and DNA joining reactions that complete the microhomology-mediated pathway of nonhomologous end join

189 sm while possibly suppressing an alternative microhomology-mediated pathway.

190 udies are elucidating the characteristics of microhomology-mediated pathways, which are mutagenic.

191 r the bulk of germline structural variation: microhomology-mediated processes involving short (2-20 b

192 ich suggests its involvement in a non-random microhomology-mediated recombination generating the rear

193 A distinct mechanism namely microhomology-mediated recombination occurs between a fe

194 gulation of many key genes, and an inducible microhomology-mediated recombination pathway could be a

195 n events at random non-restriction sites via microhomology-mediated recombination.

196 appearance from some species is occurring by microhomology-mediated recombination.

197 at the Igh locus increases DSB resolution by microhomology-mediated repair while decreasing C-NHEJ ac

198 These rearrangements were resolved by microhomology-mediated repair, which suggests that L1-as

199 complex duplication-associated variants was microhomology-mediated repair.

200 AHR), non-homologous end joining (NHEJ), and microhomology-mediated replication-dependent recombinati

201 However, a modest shift toward microhomology-mediated switch junction formation was obs

202 Small, approximately 10-kilobase microhomology-mediated tandem duplications are abundant

203 Importantly, we show that these microhomology-mediated template switches, indicative of

204 Especially prominent are microhomology-mediated template switches.

205 defective DNA replication initiates serial, microhomology-mediated template switching (chromoanasynt

206 ss mechanism terminated by end joining or by microhomology-mediated template switching, the latter fo

207 hed DSBs are repaired by a highly mutagenic, microhomology-mediated, alternative end-joining pathway,

208 t and a DNA repair signature consistent with microhomology-mediated, break-induced replication.

209 y C-NHEJ to form junctions either with short microhomologies (MHs; "MH-mediated" joins) or no homolog

210 ions have between 0 and 4 base pairs (bp) of microhomology (n = 26), short inserted sequences (n = 8)

211 r size distribution, which frequently showed microhomology near the breakpoints resembling repair by

212 o be important for processing ends to expose microhomologies needed for NHEJ.

213 ith no homology through resection to uncover microhomologies of a few nucleotides.

214 urate repair of an I-SceI DSB lacking nearby microhomologies of greater than four nucleotides in Dros

215 All deletion termini showed microhomologies of two to six nucleotides.

216 s and showed a preference for annealing at a microhomology of 8 nt buried within the DNA substrate; t

217 tions of up to 302 bp, annealed by imperfect microhomology of about 8 to 10 bp at the junctions.

218 e rejoining of DSBs that utilized a recessed microhomology or DSBs bearing 5'-hydroxyls but no gap.

219 gating two free DNA ends of little homology (microhomology) or DNA ends of no homology.

220 uman Polmu promoted microhomology search and microhomology pairing between the primer and the templat

221 ype and, importantly, a similar long S joint microhomology phenotype was observed in both Msh5 and Ms

222 roceed either by homologous recombination or microhomology-primed re-initiation.

223 earch to removal of non-homologous tails and microhomology-primed synthesis across broken ends.

224 one-dimensional sliding, and length-specific microhomology recognition to efficiently align DNA seque

225 igned the primer to achieve annealing with a microhomology region in the template several nucleotides

226 to stabilize duplexes between pairs of short microhomology regions, thereby impeding short-range reco

227 We demonstrate that microhomology-rich S-S junctions are enriched in cells i

228 As a result, human Polmu promoted microhomology search and microhomology pairing between t

229 mologies and is made efficient by coupling a microhomology search to removal of non-homologous tails

230 joining and V(D)J recombination through its microhomology searching and pairing activities but do no

231 iency of Msh2 does not lead to the increased microhomology seen with Mlh1 or Exo1 deficiencies, sugge

232 ts showing eight to 43 base pairs of perfect microhomology, suggesting replication error Microhomolog

233 SR events have similar lengths of junctional microhomology, suggesting trans-CSR occurs by nonhomolog

234 Microhomology (TAA and AGCT) at the breakpoints indicate

235 -S junctions induced by DeltaAID have longer microhomologies than do those induced by full-length AID

236 e of Msh2 results in CSR junctions with less microhomology than joinings that occur when MMR is initi

237 umber with inversions, deletions, and 5'-end microhomologies to the target DNA sequence.

238 and deletional NHEJ (D-NHEJ), which utilizes microhomology to join the ends with small deletions.

239 n homologs versus sister chromatids by using microhomology to prime DNA replication-a prediction of t

240 A survey of microhomologies typically revealed sequences of between

241 ases solve this problem by searching in 8-nt microhomology units, reducing the search space and accel

242 oth 53BP1 and BRCA1 increases repair needing microhomology usage and augments loss of DNA sequence, s

243 -EJ) can operate and exhibits a trend toward microhomology usage at the break junction.

244 ly, 60% of patients presented with increased microhomology use at switched regions.

245 Furthermore, increased use of long microhomologies was found at recombination junctions der

246 alt-EJ depends on annealing at pre-existing microhomologies, we examined inaccurate repair of an I-S

247 Very short sequence elements or microhomologies were also identified.

248 AT-to-CG transversions and deletions at microhomologies were enhanced modestly by AZT.

249 Insertions and microhomologies were found at the breakpoint junctions,

250 Small deletions and microhomology were present in most junctions; insertions

251 of the deletion breakpoints have 1-30 bp of microhomology, whereas 33% of deletion breakpoints conta

252 tic selection of 8-nucleotide (nt) tracts of microhomology, which kinetically confines the search to

253 y of illegitimate transformants there was no microhomology with the integration site.