ライフサイエンスコーパス: microinjection

1 7/4-1BB) directly in the NOD mouse by embryo microinjection.

2 e aversion was blocked by intra-mPFC nor-BNI microinjection.

3 DHK also enhanced responses to glutamate microinjection.

4 which are not susceptible to transfection or microinjection.

5 d protocols which require electroporation or microinjection.

6 degree of mosaicism when compared to zygote microinjection.

7 lcium-ionomycin, ionomycin, or hPLCzeta cRNA microinjection.

8 mprovement in embryo viability compared with microinjection.

9 ith high efficiency and controlled volume is microinjection.

10 LSr neurons with local baclofen and muscimol microinjection (0.3/0.03 nmol) blocks expression of Fos

11 Microinjections (1 mul) of L-732138 (50 nm) and SB222200

12 egulatory cold defense, by means of repeated microinjections (100 nl) of the GABA(A) agonist muscimol

13 Second, OVLT microinjection (20 nl) of 1.0 m NaCl significantly raise

14 eted to endolysosomes, we used intracellular microinjection and concurrent imaging methods to test th

15 ly: for 24 h immediately following a control microinjection and for an additional 24h after a second

16 We then tested enriched features by embryo microinjection and functional tests of multiple protein

17 se to its protein inhibitor was initiated by microinjection and monitored by Forster resonance energy

18 ploid Xenopus laevis that is widely used for microinjection and tissue explant-based protocols, and t

19 ovements of FT/FTL2 were further observed by microinjection and trichome rescue studies, which reveal

20 SD in murine hippocampal slices by focal KCl microinjection and visualized the ensuing beading of den

21 Here, we used single-cell microinjections and advanced 3D imaging and analysis tec

22 verexpression mouse model 4 weeks after aSyn microinjections and after the onset of symptomatic forep

23 reticulospinal cells responded to glutamate microinjections and the size of the responses paralleled

24 recombination (HR) template vectors, embryo microinjection, and detection of mutations and insertion

25 transgene expression with classic pronuclear microinjection, and it offers comparable efficacies (num

26 transgene expression with classic pronuclear microinjection, and it offers comparable efficacies to l

27 imaging, immunohistochemistry, AAV-FLEX-GFP microinjections, and crosses to RiboTag, Ai95, and new C

28 l half of medial shell, where opioid agonist microinjections are known to enhance positive hedonic or

29 nt to sustain whole-body general anesthesia; microinjection as little as 0.5 mm off-target did not.

30 eins can be confirmed by using CyaA, GSK, or microinjection assays.

31 Downregulation of Orai1 expression by siRNA microinjection blocked Ca(2+) influx after store depleti

32 arget activity in cells could be achieved by microinjection compared with nucleofection.

33 New adjuvants, formulations, microinjection devices, and skin delivery techniques for

34 Moreover, BDNF microinjection directly into the VMN also lowered fastin

35 The behavioral effects of NMU microinjection directly to the NAcSh were investigated u

36 ited cocaine-induced drug-seeking, while RGD microinjection during extinction training was without co

37 In a zebrafish microinjection experiment, dre-miR-140-5p is shown to ex

38 uronal anatomical tracing, intrahypothalamic microinjections, extracellular single-unit recordings of

39 and Y2R) receptors infused via intrastriatal microinjection followed by a bolus of METH (30 mg/kg, ip

40 Finally, estradiol microinjections followed by microdialysis were used to d

41 ghly efficient with the potential to replace microinjection for in vivo genome editing in mice and po

42 y to consume tasty foods and where mu-opioid microinjections generate intense motivational 'wanting'

43 Kif2a depletion by siRNA microinjection generated severely defective spindles and

44 After microinjection, glomeruli were capable of engrafting on

45 ques such as electroporation, lipofection or microinjection have been developed to overcome the cellu

46 In naive animals, OXT microinjection in the dorsal vagal complex induced a NO-

47 loped methods for gene transduction by viral microinjection in the epithelium of cultured Neurog3-nul

48 ring localized vascular injury with thrombin microinjection in the mesenteric circulation of mice, we

49 mption was demonstrated by observations that microinjection in the same anterior dorsomedial quadrant

50 ity were recorded after N-methyl-d-aspartate microinjection in the SNpc and/or optogenetic stimulatio

51 ameters were significantly influenced by the microinjections in a biphasic dose-response relationship

52 urrent study examined the effects of similar microinjections in euthyroid rats.

53 We used simultaneous microinjections in medial prefrontal cortex regions and

54 ion and for an additional 24h after a second microinjection including a T3 dose to the preoptic regio

55 rmic (30 degrees C) culture of zygotes after microinjection increased HDR efficiency for some loci.

56 plasma insulin secretion, whereas only APDC microinjections increased PES.

57 Exendin-4 microinjections increased plasma insulin.

58 Here, we describe a novel and simple microinjection-independent technique, called Genome-edit

59 expression selectively in the sMic lineage: microinjection into a Sp fertilized egg of an RNA that c

60 rapid and potent killing phenotype following microinjection into an insect host.

61 emonstrates that TALE nuclease and donor DNA microinjection into rat zygotes results in efficient and

62 int stress and nonrestraint rats after N/OFQ microinjection into the CeA.

63 geting MALAT1 RNA, delivered by transuterine microinjection into the mouse amniotic cavity at embryon

64 st treatment, by administering an additional microinjection into the mPFC immediately prior to ED tes

65 NAC microinjection into the NAcore inhibited the reinstateme

66 ZIKV microinjection into the somatosensory cortex on one side

67 reduced rhythmic burst amplitude after AMPA microinjection into the XIIMN.

68 We applied TALE nucleases and donor DNA microinjection into zygotes to generate HDR-modified rat

69 Microinjections into CNA were conducted at one dosage ra

70 In this study, we use microinjections into electrophysiologically defined prim

71 Site-specific microinjections into M1 demonstrated that l-DOPA-induced

72 withdrawal behavior is absent with analogous microinjections into the lateral habenula of nicotine-tr

73 We used in vivo microinjections into the preBotC and in vitro isolated b

74 food-seeking experiments indicate that PEPA microinjections into the RMTg did not influence the exti

75 However, microinjection is a technically demanding, labor-intensi

76 Microinjection is considered the gold standard technique

77 Finally, scopolamine microinjections localized to the caudal half of medial s

78 ng rats of both sexes, we applied a modified microinjection method that permitted localization of the

79 jor subtypes of opioid receptors via agonist microinjections [mu (DAMGO), delta (DPDPE), or kappa (U5

80 ted mitochondria through appropriately sized microinjection needles into rodent oocytes or single-cel

81 ipped with epifluorescence and injected with microinjection needles using a picospritzer forced-air i

82 o the large size of mitochondria relative to microinjection needles.

83 g in the contralateral hemisphere, after the microinjection of 10 mug of LPS.

84 Microinjection of 70 mM potassium in physiological buffe

85 Bilateral microinjection of [Pyr(1) ]apelin-13 into the rostral ve

86 Bilateral RVLM microinjection of [Pyr(1) ]apelin-13 significantly incre

87 n GEPRs was evaluated, using focal bilateral microinjection of a cannabinoid (CB1) receptor antagonis

88 For Cre lines generated via pronuclear microinjection of a Cre transgene construct, the integra

89 Importantly, microinjection of a Cre-inducible ErbB4 virus (AAV-ErbB4

90 This technique involved the microinjection of a fluorescent phosphopeptide that is h

91 Mice underwent stereotaxic microinjection of a herpes simplex virus expressing eith

92 genome editing is typically accomplished by microinjection of a mixture of Cas9 DNA/mRNA and single-

93 Using microinjection of adeno-associated viral vector bearing

94 In addition, microinjection of AIP into the PVN significantly reduced

95 ric measurement of heart rate, indicate that microinjection of aldosterone into the nucleus ambiguus

96 We show here that microinjection of amniotic fluid into the fetal (embryon

97 1 antibody, depolymerizing microtubules, or microinjection of an antibody that inhibits kinesin, VE-

98 In addition, the microinjection of an oligodeoxyribonucleotide (OD) direc

99 Furthermore, bilateral microinjection of ANA-12 into the dmNTS greatly diminish

100 d demonstration that the pressor response to microinjection of angiotensin II into the rostral ventro

101 aIII spectrin using siRNA technology and the microinjection of anti-betaIII spectrin antibodies into

102 Bim silencing or microinjection of anti-Bim antibodies into the cell cyto

103 These effects were phenocopied by microinjection of anti-H3T3ph antibodies.

104 Accordingly, local microinjection of anti-MFG-E8 mAb exacerbated periodonta

105 Tissue-targeted loss-of-function assays (via microinjection of antisense morpholino or CRISPR-Cas9) c

106 Brainstem microinjection of APDC or L-AP4 decreased plasma insulin

107 Moreover, microinjection of artificial cerebrospinal fluid at a pH

108 d completely prevented it when combined with microinjection of autophagy-targeting antibodies specifi

109 Conversely, in AP rats, microinjection of bicuculline had no effect, whereas kyn

110 In control rats, microinjection of bicuculline into the DMV increased PES

111 genome editing is typically accomplished by microinjection of Cas9 DNA/RNA and single guide RNA (sgR

112 rate of Fah gene targeting was achieved with microinjection of Cas9 mRNA, gRNA and single strand olig

113 lls in bone marrow chimeras or intracerebral microinjection of CCR4-competent DCs, but not macrophage

114 Microinjection of CelTOS into cells resulted in observab

115 Interestingly, microinjection of ChABC close to dendritic segments was

116 of pancreatogenesis-disabled sheep by oocyte microinjection of CRISPR/Cas9 targeting PDX1, a critical

117 In current clamp recordings, microinjection of cross-linked 300 kDa increased excitab

118 In addition, microinjection of D-serine into the SCN also increased C

119 We demonstrate that microinjection of DNA constructs into fertilized one-cel

120 n contrast, no effect was observed following microinjection of doses that are not thought to block as

121 Microinjection of double-stranded RNAs targeting ku70 or

122 Using in vivo microdialysis and microinjection of drugs into the mesolimbic DA system, w

123 ht to block astrocytic channels or following microinjection of either dose into the nucleus accumbens

124 We describe a protocol for microinjection of embryos for an emerging model system,

125 We show that microinjection of endothelial cells with a monoclonal an

126 tivation of VMH EphA5 receptors via targeted microinjection of ephrinA5-Fc before a hyperinsulinemic

127 rague-Dawley rats that received an acute VMH microinjection of ephrinA5-Fc, chronic VMH knockdown, or

128 We recently reported that microinjection of ethanol into the rostral ventrolateral

129 scular parabiosis coupled with intravascular microinjection of fluorescent bioparticles and liposomes

130 frogs, drug inhibition or overexpression by microinjection of formin has a chirality-randomizing eff

131 Remarkably, microinjection of free lysine (K) 63-linked ubiquitin ch

132 Microinjection of GABA(A) receptor antagonists into PnO

133 r 3 weeks abstinence was increased following microinjection of gap-junction hemichannel blockers into

134 e achieved in human cells using glass-needle microinjection of genome editing reagents.

135 Bilateral microinjection of glycine into the SCN elevated CBF in a

136 xtracellular administration or intracellular microinjection of GPR55 ligands.

137 Microinjection of high concentrations of the peptide GsM

138 n days later, mice received intradermal (id) microinjection of histamine, chloroquine, capsaicin, or

139 Microinjection of ICI 174,864, a delta-opioid receptor a

140 ssion and knockdown of gene function through microinjection of in vitro-translated mRNAs or gene-spec

141 novel in vivo chemotaxis assay, perivenular microinjection of inflammatory mediators induced directi

142 CSD, elicited by pressure microinjection of KCl, was recorded in anesthetized rats

143 Microinjection of ketamine into the prelimbic (PL) regio

144 Microinjection of l-DOPA directly into the striatum amel

145 In contrast, bilateral microinjection of L741,626 into the PFC (but not striatu

146 e induced in the rat spinal dorsal column by microinjection of lipopolysaccharide, and examined immun

147 s the Ca(2+) signal induced by intracellular microinjection of LPI converges to hyperpolarization of

148 Microinjection of LY37 into BA at both nM and mM concent

149 of IL-11Ralpha(-/-) mice using stereotactic microinjection of lysolecithin were larger than in contr

150 on in the mouse spinal cord dorsal column by microinjection of lysophosphatidylcholine (LPC).

151 Second, in rats, we demonstrated that the microinjection of MCH into the lateral ventricle results

152 Microinjection of MECP2-targeting TALEN plasmids into rh

153 secretion (PES) and plasma insulin following microinjection of metabotropic glutamate receptor (mGluR

154 Conversely, microinjection of MFG-E8 inhibited bone loss in experime

155 Intraspinal stereotaxic microinjection of MIF resulted in upregulation of inflam

156 Finally, microinjection of miR-153 in the region of mouse first m

157 Microinjection of miR-30 mimics into zebrafish embryos r

158 Direct microinjection of mitochondria into typical mammalian ce

159 genesis, electroporation of plasmid DNA, and microinjection of morpholinos are all routinely employed

160 Co-microinjection of mouse embryos with Cas9 mRNA and singl

161 e human embryonic stem cell-based system and microinjection of mouse zygotes.

162 the CeA or BLA in macaques by intracerebral microinjection of muscimol (to inactivate) or bicucullin

163 ssium currents were significantly reduced by microinjection of mutant G85R SOD1YFP that had been prei

164 Local microinjection of Myo1c promoted G-actin accumulation an

165 In addition, microinjection of NAS into the PVN decreased blood press

166 nic technology such as isolation of zygotes, microinjection of NAs into them, and their subsequent tr

167 oline as well as dynamic changes elicited by microinjection of neostigmine, an inhibitor of acetylcho

168 stimulation of mossy fibers (MFs) as well as microinjection of NMDA in the granular layer generates b

169 tic tone and sympathoexcitatory responses to microinjection of NMDA in the PVN of rats with CHF.

170 Here, we show that NAc microinjection of orexin-A in medial shell amplifies the

171 o stimulate in vivo tumor growth early after microinjection of OVCAR3 cells in nude mice.

172 Following CRF pretreatment, however, microinjection of OXT attenuated or, at times reversed,

173 Microinjection of OXT in the DVC decreased gastric tone

174 Microinjection of pioglitazone into the AMY, but not int

175 Consistent with this finding, site-specific microinjection of pioglitazone into the RMTg but not int

176 Systemic or local microinjection of PKR inhibitor to the gustatory cortex

177 Importantly, localized microinjection of PMN-MPs into wounded colonic mucosa wa

178 Inhibiting dynamin function by microinjection of purified dynamin antibodies increases

179 Microinjection of RO5166017 into the NAc core and shell

180 The results showed that microinjection of RO5166017 into the VTA and PrL decreas

181 nforced operant responding was unaffected by microinjection of RO5166017 into these brain regions.

182 might be present in adults since unilateral microinjection of ROCK or MLCK inhibitors into the hypog

183 Bilateral microinjection of S33084 (2.5 mug/side) into the prefron

184 Microinjection of Shp2(D61A)-expressing adeno-associated

185 uppression of Ku70 concurrent with embryonic microinjection of site-specific nucleases yielded consis

186 ction of an anesthesia-like state in rats by microinjection of small amounts of GABAA-receptor agonis

187 Microinjection of small amounts of human full-length DUX

188 Microinjection of somatostatin (SST) causes site-specifi

189 was induced in hippocampus or VTA of rats by microinjection of specific lentiviral vectors.

190 ibility of achieving targeted mutagenesis by microinjection of TALEN mRNA within the mouse oocyte.

191 In contrast, intra-VTA microinjection of tatCN21 just before the CPP test did n

192 Conversely, microinjection of the adenylyl cyclase inhibitor SQ22536

193 Microinjection of the agonists (MT-II and alpha-MSH) int

194 Here, we show that microinjection of the AMPAR antagonist NBQX into the NAc

195 Microinjection of the GABA agonist muscimol (250 pmol) i

196 Microinjection of the GABA antagonist bicuculline (BIC;

197 on also decreased antinociception induced by microinjection of the GABAA receptor antagonist bicucull

198 Microinjection of the glutamate antagonist, kynurenic ac

199 Here we show that microinjection of the glycosyltransferase domain Afp18(G

200 Significantly, microinjection of the HCN blocker ZD7288 into the ACC in

201 ffect of donepezil was markedly reduced by a microinjection of the M2 antagonist, methoctramine, with

202 Consistent with these results, intra-shell microinjection of the mGluR1/5 agonist DHPG (250 mum) pr

203 spring stress responsivity and, using zygote microinjection of the nine specific miRs, demonstrated a

204 WKY rats, and this effect was eliminated by microinjection of the NMDAR antagonist into the PVN.

205 Microinjection of the PLCzeta EF-hand mutants into mouse

206 However, microinjection of the progesterone receptor antagonist,

207 Two patients had undergone microinjection of the same branded topical moisturizer (

208 Microinjection of the vectors into zygotes and transfer

209 Microinjection of this virus into the C3 nucleus enabled

210 Microinjection of ticks with IFNgamma induced IGTPase, a

211 or the production of mouse disease models by microinjection of transcription activator-like effector

212 neurons, whereas in vivo Fkbp1b knockdown by microinjection of viral vector (3-4 weeks) dramatically

213 sicular stomatitis virus (VSV), we show that microinjection of VSV particles leads to a dose-dependen

214 Pronuclear microinjection of ZFNs, shown by our data to be an effic

215 eration of tissue-specific knockout rats via microinjection of zinc-finger nucleases (ZFNs) into fert

216 glutamate receptors within the same region, microinjections of 1 mum substance P or 1 mm nicotine in

217 privation in the PeH or RVLM was elicited by microinjections of 2-deoxy-D-glucose or 5-thio-D-glucose

218 Microinjections of a D1 antagonist in the MLR decreased

219 odor during sleep was preceded by bilateral microinjections of a protein synthesis inhibitor into th

220 n (STZ)-diabetic rats received bilateral VMH microinjections of an adenoassociated viral vector conta

221 Subsequent intraaccumbens microinjections of an opioid-stimulating drug increased

222 easured BRS before and after bilateral dmNTS microinjections of ANA-12.

223 The present report describes the results of microinjections of anterograde tracers placed at differe

224 t instrumental outcomes, we assessed whether microinjections of DA agents into the NAc shell would im

225 n CFA-treated females that was reversed with microinjections of DS2 directly into the vlPAG.

226 Intrasubthalamic microinjections of either an inverse agonist of D5Rs, fl

227 Results showed that lesions of the mPOA or microinjections of estradiol directly into the mPOA incr

228 We gave hippocampal microinjections of FKBP1b-expressing viral vector to mal

229 Microinjections of fluorescent tracers, along with gold-

230 In contrast, microinjections of glutamate agonists enhanced the respi

231 Microinjections of glutamate and muscimol to activate or

232 Excitation of CVMs was attempted by microinjections of glutamate into the nucleus ambiguus o

233 Bath-application or local microinjections of glutamatergic antagonists markedly re

234 e nucleus (KF) were inhibited with bilateral microinjections of isoguvacine (50-70 nl, 10 mm) to remo

235 uculline into the DMV increased PES, whereas microinjections of kynurenic acid had no effect.

236 orated the dystonic movements but cerebellar microinjections of l-DOPA had no effect.

237 Previous work has shown that acute microinjections of l-triiodothyronine (T3) to the preopt

238 Unilateral microinjections of lidocaine, muscimol, or glutamate ant

239 Different groups of rats received bilateral microinjections of LY37 into BA at two dosage ranges (3.

240 Microinjections of LY37 or LY34 into CNA had no signific

241 Previously, we demonstrated that microinjections of MCH into the DRN resulted in an incre

242 Furthermore, intra-VTA microinjections of mGluR1 antagonist JNJ16259685 and pro

243 Repeated microinjections of morphine or the adenylyl cyclase acti

244 We examined whether microinjections of orexin-A into the VP hotspot enhance

245 We also show that intra-VTA microinjections of PDE4 inhibitor rolipram impaired the

246 ty in the hippocampus was modulated by focal microinjections of potassium chloride into the nucleus r

247 Microinjections of SB-334867 (20 nmol) bilaterally into

248 We report that intra-VTA microinjections of tatCN21 before cocaine conditioning b

249 cquisition of cocaine CPP, whereas intra-VTA microinjections of tatCN21 before saline conditioning di

250 ently assessed the effects of intra-NAc core microinjections of the A(2A) receptor agonist, CGS 21680

251 Pre- or post-training intra-RMTg microinjections of the allosteric AMPA receptor potentia

252 In contrast, microinjections of the antagonist scopolamine at all sit

253 In experiment 1, microinjections of the D(1) antagonist SCH-23390 into th

254 Previous studies have shown that microinjections of the GABA-A agonist muscimol into the

255 Microinjections of the GABAA agonist muscimol into PVN i

256 DMV microinjections of the group II mGluR agonist APDC and w

257 Microinjections of the KCa2 channel inhibitor apamin int

258 Stereotaxic microinjections of the long-lasting KOR antagonist norbi

259 ant P450s, the effects of intracerebral (ic) microinjections of the P450 inhibitor CC12 were determin

260 Furthermore, intra-RVM microinjections of the PN decomposition catalyst Fe(III)

261 layed sensitivity to capsaicin and brainstem microinjections of these neuropeptides induce GI effects

262 development of morphine tolerance, and vlPAG microinjections of TLR4 agonists dose dependently produc

263 one and the gastric motility response to DMV microinjections of TRH were decreased significantly.

264 ciceptive neurons were used to guide precise microinjections of various tracers in VMpo.

265 Adult male rats received bilateral microinjections of vector control or short hairpin RNA t

266 h female rats received bilateral dorsal mPFC microinjections of viral constructs coding light-sensiti

267 Male Long-Evans rats received intra-BLA microinjections of viral vectors carrying either halorho

268 Next, we evaluated the effects of NMU microinjection on behavioral sensitization to cocaine.

269 Despite many microinjections on the transgenic strains with varying c

270 e transgene expression to classic pronuclear microinjection or somatic cell nuclear transfer (SCNT),

271 approximately ten times smaller than typical microinjection pipettes and rather than pressure pulses

272 The transposase-enhanced pronuclear microinjection (PNI) technique described herein uses the

273 The catheters were sealed with microinjection ports and then implanted subcutaneously.

274 versely, CeA inhibition by muscimol/baclofen microinjections prevented acquisition of cocaine self-ad

275 MII oocyte microinjection reduced lysis, improved blastocyst rate,

276 I depolarizes PAG neurons and upon intra-PAG microinjection, reduces nociceptive threshold in the hot

277 Regional microinjections revealed that N/OFQ attenuated dyskinesi

278 Moreover, blastocyst microinjection showed that the iMuSCs contributed to chi

279 In 7 rats with histologically confirmed microinjection sites bilaterally placed in the preoptic

280 By contrast, microinjection sites in the anterior half of VP, or in L

281 Following microinjection, sleep was recorded for 20 h.

282 at all sites throughout medial shell, orexin microinjections stimulated 'wanting' to eat, as reflecte

283 Our in vivo microinjection studies of high molecular weight cell lin

284 Comparative In Vivo Oncology (CIVO) arrayed microinjection technology to test tumor responsiveness t

285 e targeted CpG methylation in mice by zygote microinjection, thereby demonstrating its potential util

286 Since it does not require electroporation or microinjection, this tool has the potential to be applie

287 We used microinjection to infect the organoids with H pylori.

288 bility of NAc core GLP-1R activation by Ex-4 microinjection to suppress food intake and body weight g

289 Furthermore, NMU microinjection to the NAcSh decreased local gamma-aminob

290 al time of 5-HT and noradrenaline applied by microinjection to the NTS.

291 These effects of T3 microinjection to the preoptic region were demonstrated

292 We performed fluorescent dye microinjections to identify aortic arch patterns and mea

293 grade tracing with fluorescent intracellular microinjections to perform three-dimensional reconstruct

294 ing-free single-guide RNA (sgRNA) synthesis; microinjection; validation of the target-specific activi

295 Minimal effects of lateral ventricle T3 microinjection were demonstrated (N=5).

296 Microinjections were paired, one made during pacing to m

297 ntagonist, SAFit2, in wild-type mice via BLA microinjections, which reduced anxiety-related behavior.

298 termed injectoporation that combines tissue microinjection with electroporation to express cDNAs and

299 ) Rs were expressed in Xenopus oocytes after microinjection with membrane fractions from either HH or

300 As predicted, microinjection with unmodified or lesion-containing CRE,