ライフサイエンスコーパス: microneme

1 he parasite surface after discharge from the micronemes.

2 ns present in the rhoptries, rhoptry neck or micronemes.

3 nete apical surface, and both are present in micronemes.

4 P140/RON4 is located within the rhoptries or micronemes.

5 bility and secretion of the complex from the micronemes.

6 hereas adhesins were sequestered in internal micronemes.

7 ossibly localize in the sporozoite secretory micronemes.

8 ce during or preceding the biogenesis of the micronemes.

9 rolled secretion of apical organelles termed micronemes.

10 ergy metabolism rather than interacting with microneme adhesins, challenging the current model for ap

11 reveal a novel cell-binding motif called the microneme adhesive repeat (MAR).

12 s pinpointed to a defect in secretion of the micronemes, an apicomplexan-specific organelle that cont

13 antigen 1 (AMA1) is located in the merozoite micronemes, an organelle that contains receptors for inv

14 type and ASP3 depleted parasites identified microneme and rhoptry proteins as repertoire of ASP3 sub

15 Fluorescence microscope analysis detected microneme and surface antigen proteins on the monocyte c

16 These results suggest that microneme and surface antigen proteins trigger monocytes

17 berghei we show that SOAP is targeted to the micronemes and forms high molecular mass complexes via d

18 Regulated protein secretion from micronemes and host cell invasion are inextricably linke

19 leading to reduced TgMIC2 secretion from the micronemes and impaired invasion.

20 Micronemes and rhoptries are specialized secretory organ

21 wly formed apical secretory organelles named micronemes and rhoptries in the daughter cells.

22 of P. gallinaceum is present within ookinete micronemes and subsequently becomes localized in the ele

23 onserved apicomplexan protein present in the micronemes and then secreted onto the surface of the mer

24 st cell adhesins from apical organelles (the micronemes), and extension of a unique tubulin-based str

25 Rhoptries, micronemes, and dense granules, which are secretory orga

26 onstrated to be GPI-linked, localized in the micronemes, and essential for erythrocyte invasion.

27 Proteins secreted from the apical micronemes are central components for host cell recognit

28 MIC2 and M2AP are stored in micronemes as proforms.

29 e replication and initially localizes to the micronemes, as determined by immunofluorescence assay an

30 acking the C-domain might be escorted to the micronemes by association with endogenous wild-type MIC2

31 ected in the mutant implying that release of microneme cargo is either highly efficient at the malfor

32 ibution of their micronemes yet secretion of microneme cargo is unaffected in the mutant implying tha

33 complex is the most extensively investigated microneme complex, which contributes to host cell recogn

34 e aldolase to the periphery of the secretory micronemes containing TRAP.

35 Within the microneme CTRP was associated peripherally at the micron

36 from Toxoplasma apical secretory organelles (micronemes, dense granules, and rhoptries) play key role

37 lysis using antibodies against proteins from micronemes, dense granules, rhoptries, and plasma membra

38 s the functional activity of PfCDPK1, blocks microneme discharge and erythrocyte invasion by P. falci

39 specific inhibitor of PfCDPK1, also inhibits microneme discharge and erythrocyte invasion, confirming

40 Here, we demonstrate that microneme discharge is regulated by parasite cytoplasmic

41 hanism in which key lipid mediators regulate microneme exocytosis.

42 ing microneme secretion and is necessary for microneme exocytosis.

43 tidic acid, a lipid mediator associated with microneme exocytosis.

44 eins in apical secretory organelles known as micronemes have been strongly implicated in parasite att

45 PvGAMA was localized at the microneme in the mature schizont-stage parasites.

46 x Duffy binding protein (DBP) is a merozoite microneme ligand vital for blood-stage infection, which

47 When microneme-mediated attachment was blocked by pretreating

48 neme CTRP was associated peripherally at the microneme membrane, whereas PgCHT1 and WARP were diffuse

49 ive proteins from internal organelles called micronemes, no genetic evidence is available to support

50 erythrocyte binding proteins located in the micronemes of merozoites.

51 is and remains associated with TgMIC2 in the micronemes, on the parasite surface during invasion and

52 Microneme organelles are found in the apical complex of

53 Both EBA-175 and BAEBL localize to the micronemes, organelles at the invasive ends of the paras

54 Our findings suggest that processing of microneme precursors occurs within intermediate endocyti

55 nvasion factor the claudin-like apicomplexan microneme protein (CLAMP), which resembles mammalian tig

56 ells by a multistep process with the help of microneme protein (MIC) complexes that play important ro

57 process involving the secretion of adhesive microneme protein (MIC) complexes.

58 One of these adhesins, microneme protein 2 (MIC2), is a type one transmembrane

59 orthologs, including Toxoplasma gondii MIC2 (microneme protein 2), possess a short cytoplasmic tail,

60 The SP from microneme protein EtMIC2 (SP2) allowed efficient traffic

61 TgM2AP is a propeptide-containing microneme protein found in a heterohexameric complex wit

62 Several antigens, including the microneme protein MIC2, the cyst matrix protein MAG1, an

63 ghei, we identify and characterize the first microneme protein of the ookinete: circumsporozoite- and

64 oplasma gondii, an essential activity called microneme protein protease 1 (MPP1) cleaves secreted adh

65 found in a heterohexameric complex with the microneme protein TgMIC2, a protein that has a demonstra

66 , we report successful disruption of M2AP, a microneme protein tightly associated with an adhesive pr

67 TgMIC2 is a microneme protein with multiple adhesive domains that bi

68 similar to the C-terminal domain of another microneme protein, MIC1.

69 econd example of a galectin fold adapted for microneme protein-protein interactions and suggest a con

70 Calcium-regulated secretion of microneme proteins and parasite actin polymerization tog

71 Remarkably, most microneme proteins are proteolytically cleaved during bi

72 s processing of several secreted rhoptry and microneme proteins by targeting the corresponding matura

73 is a candidate processing enzyme for several microneme proteins cleaved within the secretory pathway

74 The recent identification of microneme proteins from different apicomplexan genera ha

75 Secretory microneme proteins have been shown to be important for b

76 Apicomplexans possess a large repertoire of microneme proteins that contribute to invasion, but thei

77 High-level Ab responses to rhoptry and microneme proteins that function in erythrocyte invasion

78 sed by its ability to block the secretion of microneme proteins, which are involved in cell attachmen

79 c residues are similarly positioned in other microneme proteins.

80 olytic maturation of proproteins targeted to micronemes, regulated secretory organelles that deliver

81 These studies indicate that microneme release is a stimulus-coupled secretion system

82 idic acid (PA), is essential for controlling microneme release.

83 t secretion of specialized organelles called micronemes, resulting in a block of essential phenotypes

84 of proteins from three different organelles--micronemes, rhoptries and dense granules--serves to esta

85 hable from other parasite organelles, namely micronemes, rhoptries, dense granules, and the apicoplas

86 PfROM1 was not found associated with micronemes, rhoptries, or dense granules, the three iden

87 -triphosphate receptor antagonist, inhibited microneme secretion and blocked parasite attachment and

88 the microneme surface that senses PA during microneme secretion and is necessary for microneme exocy

89 n of Cys127 on TgDJ-1 resulted in a block of microneme secretion and motility, even in the presence o

90 The natural agonist serum albumin induced microneme secretion in a protein kinase G-dependent mann

91 earch for mediators of calcium signaling and microneme secretion in T. gondii.

92 Calcium-mediated microneme secretion in Toxoplasma gondii is stimulated b

93 Taken together, these findings reveal that microneme secretion is centrally controlled by protein k

94 ajor effect was on invasion, suggesting that microneme secretion is dispensable for Plasmodium egress

95 Microneme secretion is essential for motility, invasion,

96 The TgAMA1 deficiency has no effect on microneme secretion or initial attachment of the parasit

97 enetically encoded indicators for Ca(2+) and microneme secretion to better define the signaling pathw

98 Microneme secretion under the conditions of stimulation

99 nted invasion even under conditions in which microneme secretion was not affected, indicating a poten

100 Microneme secretion was triggered by Ca2+ ionophores in

101 processes in Toxoplasma gondii We found that microneme secretion was triggered in vitro by exposure t

102 xity in the control of parasite motility and microneme secretion, and they constitute a set of useful

103 Upon microneme secretion, as occurs during invasion, the MIC2

104 um with thapsagargin or NH4Cl also triggered microneme secretion, indicating that intracellular calci

105 tudies indicate that Ca(2+) and cGMP control microneme secretion, little is known about how these pat

106 downstream of the calcium flux required for microneme secretion, parasite motility, and subsequent i

107 We identified two stimuli of microneme secretion, ryanodine and caffeine, which enhan

108 hanol, a previously characterized trigger of microneme secretion, stimulated an increase in parasite

109 Upon stimulation of microneme secretion, TgSUB1 is cleaved into smaller prod

110 Similarly, microneme secretion, which is necessary for motility dur

111 otein kinase G-independent manner leading to microneme secretion.

112 host cells, which correlated with deficient microneme secretion.

113 Plasmodium falciparum was also defective in microneme secretion.

114 parasite's life cycle at stages dependent on microneme secretion.

115 ally enhance invasion, gliding motility, and microneme secretion.

116 erol (DAG) and IP3 and ultimately results in microneme secretion.

117 xan parasites have unique apical rhoptry and microneme secretory organelles that are crucial for host

118 the malaria parasite, Plasmodium, possesses microneme secretory organelles that mediate locomotion a

119 ocking the release of invasion proteins from microneme secretory organelles.

120 TRAP is located in micronemes, secretory organelles of apicomplexan parasit

121 ane protein that localizes to the parasite's micronemes, secretory organelles that discharge during i

122 (PH) domain-containing protein (APH) on the microneme surface that senses PA during microneme secret

123 h as the apicomplexan-specific rhoptries and micronemes that are required for host cell invasion.

124 m, after which the protein is transported to micronemes, vesicles that secrete early during host cell

125 Organelles consistent with rhoptries and micronemes were also present in fractions from 1.17 to 1

126 This likely mimics the release of TRAP from micronemes when a sporozoite contacts its target cell in

127 cent studies have implicated the contents of micronemes, which are small secretory organelles confine

128 ve a pronounced apical distribution of their micronemes yet secretion of microneme cargo is unaffecte