ライフサイエンスコーパス: micronuclear

1 he scrambled pattern of MDSs and IESs of the micronuclear actin I gene has been conserved during evol

2 ht hypotrichs suggests that evolution of the micronuclear actin I gene proceeds by successive additio

3 e manner during species evolution to produce micronuclear actin I genes of increasing structural comp

4 termine the architecture of four loci in the micronuclear and macronuclear genomes of the ciliate Chi

5 A single micronuclear C to T transition within the consensus sequ

6 In contrast, condensed inactive micronuclear chromatin is enriched in dephosphorylated d

7 ically, cnj1 and cnj2 fail to condense their micronuclear chromatin prior to each of the three prezyg

8 dine autoradiography, shows that decondensed micronuclear chromatin undergoing active transcription i

9 ermophila ribosomal RNA gene is excised from micronuclear chromosome 1 by site-specific cleavage at c

10 Micronuclear chromosome maintenance is also compromised.

11 cterized and each was assigned to a specific micronuclear chromosome or chromosome arm.

12 tion at the homologous site in the recipient micronuclear chromosome.

13 or proper mitotic and meiotic segregation of micronuclear chromosomes and for normal chromosome align

14 on, Euplotes crassus precisely fragments its micronuclear chromosomes and synthesizes new telomeres o

15 progressively eroded, suggesting that broken micronuclear chromosomes are not 'healed' by telomerase.

16 acronucleus in the ciliate Euplotes crassus, micronuclear chromosomes are reproducibly broken at appr

17 eplications leading to polytenization of the micronuclear chromosomes before massive DNA elimination,

18 micronuclear genome and (ii) breakage of the micronuclear chromosomes into hundreds of DNA fragments,

19 In the micronuclear chromosomes, the macronuclear-destined sequ

20 ous genotoxic stress, destabilizing all five micronuclear chromosomes.

21 lowly and incur rapid deterioration of their micronuclear chromosomes.

22 completely inhibit excision of its Mild-type micronuclear copy through multiple sexual generations.

23 The germline (micronuclear) copy of this gene is broken into 48 pieces

24 cronuclear locus leads to defective germline micronuclear division and that conjugation of two somati

25 xtra micronuclei, implying faulty control of micronuclear division in the KO cells.

26 ank the 5' ends of macronuclear sequences in micronuclear DNA (12 cases) consist of approximately 50%

27 in the 5' ends of macronuclear sequences in micronuclear DNA are islands of purine richness in which

28 signal to identify macronuclear molecules in micronuclear DNA during development.

29 generation of the macronuclear rDNA from the micronuclear DNA is proposed.

30 Cbs in macronuclear-destined versus flanking micronuclear DNA leads us to propose a model of chromoso

31 genes in the somatic macronucleus results in micronuclear DNA loss and delayed chromosome segregation

32 liminated sequences (IESs) from the germline micronuclear DNA occurs during the differentiation of a

33 The micronuclear DNA of Paramecium tetraurelia is estimated

34 f Tlr elements, a family of approximately 30 micronuclear DNA sequences that are efficiently eliminat

35 olecule, but can also reside within flanking micronuclear DNA that is eliminated during macronuclear

36 mutants lose a significant fraction of their micronuclear DNA.

37 ronucleus, with a failure to disassemble the micronuclear envelope encapsulating the chromosomal frag

38 Breakdown of the micronuclear envelope, a process associated with chromot

39 basal bodies (BBs), macronuclear envelopes, micronuclear envelopes, and contractile vacuole pores.

40 can automatically align the macronuclear and micronuclear forms of a gene, outputting the location of

41 The IESs in the micronuclear gene encoding the beta-subunit of the telom

42 after cell mating the IESs are excised from micronuclear genes and the MDSs are spliced in the seque

43 Germ line micronuclear genes in ciliated protozoa contain two type

44 In some micronuclear genes MDSs are in a scrambled disorder.

45 The four micronuclear genes that have been isolated consist of a

46 IESs are excised from micronuclear genes, and the MDSs are spliced when a micr

47 ate macronucleus-destined segments (MDSs) in micronuclear genes.

48 Germ-line (micronuclear) genes in hypotrichous ciliates are interru

49 useful sequence tags for relating macro- and micronuclear genetic, physical, and sequence maps.

50 useful sequence tags for relating macro- and micronuclear genetic, physical, and whole-genome sequenc

51 atic macronuclear genome from their germline micronuclear genome after sexual reproduction.

52 g of macro-nuclear-destined sequences in the micronuclear genome allows for their differential replic

53 ed sequences (IESs) scattered throughout the micronuclear genome and (ii) breakage of the micronuclea

54 which are dispersed in 15,000 copies in the micronuclear genome and completely eliminated during for

55 in transposition of the elements within the micronuclear genome and/or their developmentally regulat

56 ately 5%) of its precursor "silent" germline micronuclear genome by a process of "unscrambling" and f

57 atic (macronuclear) genome, derived from the micronuclear genome by fragmentation, which follows a di

58 nuclear DNA termini and eight regions of the micronuclear genome containing chromosome fragmentation/

59 major repetitive sequence components of the micronuclear genome differ in their chromatin structure

60 mena thermophila removes 34% of its germline micronuclear genome from somatic macronuclei by excising

61 In conjunction with the micronuclear genome in progress, the O. trifallax macron

62 Conversion of the germ line micronuclear genome into the genome of a somatic macronu

63 ccurs during the conversion of the germ-line micronuclear genome into the somatic genome of the new m

64 In Euplotes crassus, most of the micronuclear genome is eliminated during formation of a

65 clear genes, and the MDSs are spliced when a micronuclear genome is processed into a macronuclear gen

66 indings identify a new scrambled gene in the micronuclear genome of a spirotrichous ciliate, and sugg

67 nt effectively creates a fragile site in the micronuclear genome, providing the first evidence for a

68 Genes in the germline (micronuclear) genome of hypotrichous ciliates are interr

69 trahymena possesses two genomes: a germline (micronuclear) genome that follows a Mendelian model of g

70 to maintain appropriate telomere length and micronuclear genomic stability but does so in a manner d

71 y mapping the genomes of T. thermophila: the micronuclear (germ-line) genome, which is not transcript

72 d by developmental fragmentation of the five micronuclear (germline) chromosomes.

73 ciliates we determined the structure of the micronuclear (germline) gene encoding DNA polymerasealph

74 We report the structure of the micronuclear (germline) gene encoding the large catalyti

75 Using micronuclear (germline) transformation, we show that the

76 Mass spectrometric sequencing of micronuclear H3 identified H3K23 trimethylation (H3K23me

77 Here, we demonstrate that micronuclear H3 phosphorylation occurs at a single site

78 s is attached to H2A.X, is shown to regulate micronuclear H3-S10 phosphorylation.

79 w that the specificity of the small RNAs for micronuclear-limited sequences increases during conjugat

80 inata and compare the levels of variation in micronuclear-limited sequences to macronuclear destined

81 were used to genetically map 11 junctions to micronuclear linkage groups and macronuclear coassortmen

82 a rapid and dramatic phosphorylation of the micronuclear linker histone, delta, occurs early in the

83 Previous studies have suggested that micronuclear linker histones are phosphorylated by cAMP-

84 Micronuclear linker histones of the ciliated protozoan,

85 To investigate the relationship between the micronuclear map and coassortment groups, we systematica

86 continuous segment in a given region of the micronuclear map, with no intervening markers from other

87 genous DNA damage and is required for normal micronuclear meiosis and mitosis and, to a lesser extent

88 ring S phase, which occurs immediately after micronuclear mitosis.

89 istribute their chromosomes correctly during micronuclear mitosis.

90 nue to divide, eventually recapitulating the micronuclear mitotic defects described previously for ra

91 otrich Uroleptus sp., and then pursued their micronuclear organization.

92 s at sites of breakage flanking the germline micronuclear rDNA locus of six additional species of Tet

93 At one end of the T. thermophila micronuclear rDNA locus, a pair of short inverted repeat

94 cronuclei following the programmed arrest of micronuclear replication.

95 clear polymer is derived from regions of the micronuclear spacer on both the right and the left.

96 the macronuclear polymer is derived from the micronuclear spacer on the right, the spacer at the left

97 demonstrate that excision and elimination of micronuclear-specific DNA occurs independently of endore

98 small RNAs that hybridized preferentially to micronuclear-specific sequences and on the properties of

99 coccal nuclease-resistant DNA indicates that micronuclear telomeres are organized into a chromatin st

100 from nongenic, heterogeneous, bidirectional, micronuclear transcripts synthesized at early stages of

101 results argue that Dcl1p processes nongenic micronuclear transcripts to scnRNAs and is required for

102 like enzymes, is required for processing the micronuclear transcripts to scnRNAs.

103 This is the first evidence linking nongenic micronuclear transcripts, scnRNAs, and genome rearrangem

104 Both macronuclear and micronuclear transformants were recovered.

105 In the micronuclear version of the actin I gene intramolecular

106 The micronuclear versions of genes in stichotrichous ciliate

107 atin using hybridization probes specific for micronuclear vs. macronuclear sequences indicates that a