ライフサイエンスコーパス: micropipette

1 d tissues where cells can be targeted with a micropipette.

2 dual-beam lasers and the other was held by a micropipette.

3 0.2 M) microinjections (50 nl) from a glass micropipette.

4 ymersomes using either optical tweezers or a micropipette.

5 ssue, and individually loaded onto a suction micropipette.

6 cal acid injection via a cell-attached glass micropipette.

7 gative pressure applied to the membrane by a micropipette.

8 a single red blood cell is aspirated into a micropipette.

9 e calculated from red cell velocities in the micropipette.

10 ectroporates a single cell at the tip of the micropipette.

11 d controllably deformed by aspiration into a micropipette.

12 that was constrained to the vicinity of the micropipette.

13 ting antibodies and bending stiffness of the micropipette.

14 ylcholine injection into the compact AVN via micropipette.

15 ations of a coarse-grained network held in a micropipette.

16 particles immobilized and manipulated with a micropipette.

17 aining 0.05% Triton X-100 on it from a third micropipette.

18 simulations of erythrocyte deformation in a micropipette.

19 was measured concurrently with a servo-null micropipette.

20 ement of a microsphere by using a fine glass micropipette.

21 chemotactic efficiency as they approach the micropipette.

22 ction of these rare marrow cells using glass micropipettes.

23 of cardiac surgery and cannulated with glass micropipettes.

24 s during cardiac surgery and cannulated with micropipettes.

25 roteins directly applied to growth cones via micropipettes.

26 Neural recordings were made with glass micropipettes.

27 endent electrodes fabricated on pulled glass micropipettes.

28 by glutamate which was pressure-ejected from micropipettes.

29 ked by GABA, which was pressure-ejected from micropipettes.

30 attached patch recordings using quartz-glass micropipettes.

31 ng the local membrane elasticity measured by micropipettes.

32 responses to glutamate, applied locally via micropipettes.

33 raocular pressure was directly assessed with micropipettes.

34 endent vasoactive agents were delivered from micropipettes (0.5 or 1 second pulse) onto an arteriole

35 rom 1.8 to 12.6 mm in inner diameter (ID), 6 micropipettes (0.7- or 1.1-mm ID filled with (64)Cu at 5

36 lutamate microelectrodes equipped with glass micropipettes 50 microm from the recording surfaces were

37 12)-10(-4) mol l(-1)) was applied dose-wise (micropipette, 60 seconds).

38 After withdrawal of the micropipette, a second penetration led to a similar leve

39 When released from a micropipette, acetylcholine (ACh) triggered vasodilatati

40 ll wound is induced in an intact layer using micropipette action and responses in neighboring cells a

41 combinant PECAM as assessed in a single-cell micropipette adhesion assay able to measure the biophysi

42 he peptide:MHC monomer-based two-dimensional micropipette adhesion frequency assay confirmed that NFM

43 ested this assumption by using data from the micropipette adhesion frequency assay that generates seq

44 ractions between T cells and APCs, we used a micropipette adhesion frequency assay to measure the adh

45 The micropipette adhesion frequency assay was used to obtain

46 By using a micropipette adhesion frequency assay, we show that this

47 Utilizing the highly sensitive micropipette adhesion frequency assay, which allows one

48 plasmon resonance and in two dimensions by a micropipette adhesion frequency assay.

49 Using the two-dimensional micropipette adhesion-frequency assay, we show that TRM

50 , 100 microm AP-5, or 50 microm CNQX) from a micropipette adjacent to the recording electrode signifi

51 pricking the rear of a slug with an unfilled micropipette, also cause a more limited nuclear transloc

52 moved toward a chemoattractant emitted by a micropipette, although persistence was lower than that o

53 Using two micropipettes, an avidin-coated vesicle was presented to

54 ions, an exponential gradient emitted from a micropipette and a shallow, linear gradient in a Dunn ch

55 ed detection scheme utilizing a borosilicate micropipette and an assay of complementary gamma-peptide

56 cted by iontophoresis through a single glass micropipette and detected by immunohistochemistry.

57 battery-powered instrumentation (electronic micropipette and potentiostats) is commercially availabl

58 er-oleic acid (W-O) mixtures with the aid of micropipette and shaking.

59 n the impedance of the interface between the micropipette and the cell interior, which limits how sma

60 ce using the adhesion frequency assay with a micropipette and the thermal fluctuation and force-clamp

61 The IFP was measured with micropipettes and a servo-null system.

62 mpedance, they can be made much smaller than micropipettes and microelectrodes.

63 vessel is cannulated at both ends with glass micropipettes and the fluid filtration rate across the v

64 myocytes were introduced into the chamber by micropipetting and subsequently capped with a layer of m

65 second study, red cells are aspirated into a micropipette, and immunofluorescent maps of the surface

66 ophoretically or pressure ejected from glass micropipettes, and 7 days later the animals were euthani

67 m o.d.) were isolated, cannulated with glass micropipettes, and pressurized to 85 mmHg.

68 rom OD) were isolated, cannulated with glass micropipettes, and pressurized.

69 olated from male rats, cannulated with glass micropipettes, and pressurized.

70 Focal micropipette application of either ACh, to stimulate end

71 e, the micropipette force probe, that uses a micropipette as a flexible cantilever that can aspirate

72 This perspective highlights the relevance of micropipette-aspirated single-particle tracking-used to

73 al properties of lipid bilayers by combining micropipette aspiration (MA) with theoretical modeling.

74 also caused the projections induced both by micropipette aspiration and by lysophosphatidylcholine t

75 coupling can be obtained by a combination of micropipette aspiration and fluctuation spectroscopy mea

76 In this work, we utilize micropipette aspiration and fluorescence imaging to exam

77 First, we simulated the micropipette aspiration and quantified the cytoskeletal

78 tion, we sorted spheroids using an automated micropipette aspiration and release system and monitored

79 The dynamics of human neutrophils during micropipette aspiration are frequently analyzed by appro

80 hrough micropores and nuclear flexibility in micropipette aspiration both appear limited by lamin-A:B

81 Micropipette aspiration demonstrated decreased cortical

82 Furthermore, micropipette aspiration experiments revealed increased e

83 ed from comparable rupture times in separate micropipette aspiration experiments.

84 itatively by our atomic force microscopy and micropipette aspiration measurements of iRBCs.

85 ment with previously reported values and our micropipette aspiration measurements.

86 We used the micropipette aspiration method, by which the changes of

87 Micropipette aspiration of cells shows myosin-II accumul

88 We utilized micropipette aspiration of giant unilamellar vesicles co

89 We have utilized micropipette aspiration of giant unilamellar vesicles to

90 rotein-lamin B dynamics were visualized in a micropipette aspiration of isolated nuclei, and both wer

91 Furthermore, studies using micropipette aspiration of single vesicles reveal that c

92 fluid motion around colonies immobilized by micropipette aspiration reveals flow fields with very la

93 Our results from micropipette aspiration suggest that cholesterol and erg

94 existence of fluid and gel-like domains, the micropipette aspiration technique allowed measurements o

95 In this study, using the micropipette aspiration technique and fluorescence micro

96 In this paper, we use the micropipette aspiration technique to characterize the el

97 The micropipette aspiration technique was used to determine

98 a custom-designed microscope chamber for the micropipette aspiration technique.

99 tension experiments at 37 degrees C with the micropipette aspiration technique.

100 uble tethers from endothelial cells with the micropipette aspiration technique.

101 , we examined EC surface protrusion with the micropipette aspiration technique.

102 ong the three layers were evaluated with the micropipette aspiration technique.

103 to investigate by alternative methods, i.e., micropipette aspiration technique.

104 s or overexpresses various myosin I's, using micropipette aspiration techniques.

105 We used micropipette aspiration to directly measure the area com

106 oplasmic membranes by applying the method of micropipette aspiration to Escherichia coli spheroplasts

107 Employing micropipette aspiration to mimic squeezing through narro

108 We employ micropipette aspiration to show that anisotropic tension

109 Micropipette aspiration was used to measure the pressure

110 Micropipette aspiration was used to test mechanical stre

111 on analysis, vesicle electrodeformation, and micropipette aspiration were used to assess the bending

112 Local measurements by micropipette aspiration, however, have reported only an

113 Using micropipette aspiration, we show that the lamina in HGPS

114 han the value reported for intact cells from micropipette aspiration.

115 in Monte Carlo simulations of whole cells in micropipette aspiration.

116 quantify cell-substrate adhesion force using micropipette aspiration.

117 , thermal degradation, and applied stress by micropipette aspiration.

118 Here, with the micropipette-aspiration technique, we show that tethers

119 e have determined this relationship with the micropipette-aspiration technique.

120 -cell receptor (TCR) signalling reporter and micropipette assay to quantify naive precursors and expa

121 etermine molecular density (expression), and micropipette assays are used to find the probability of

122 A five barrel micropipette assembly was used for extracellular recordi

123 Glass micropipettes, atomic force microscope tips and nanoneed

124 jected into spinal segments T12-L3 through a micropipette attached to a Hamilton microliter syringe.

125 atory include traditional transfer pipettes, micropipettes based on air displacement, and motorized d

126 Micropipette-based measurements of cadherin-mediated, ce

127 liter droplets of a second reagent through a micropipette by means of a pressure-driven droplet injec

128 ese agents and K+ (150 mM) were applied from micropipettes by brief (1 s) microperfusion pulses.

129 he cell interior, which limits how small the micropipette can be.

130 define the initial contact time (+/-50 ms), micropipette cell manipulation was used to bring individ

131 pressure measurements were obtained using a micropipette connected to a servonull device and positio

132 The micropipette contained 130 mM CsCl and 1 microM QX-314.

133 emoattractant, and orient and crawl toward a micropipette containing chemoattractant.

134 led by micromanipulating bound microbeads or micropipettes, cytoskeletal filaments reoriented, nuclei

135 in chains to patterned photobleaching of the micropipette-deformed network.

136 sults for single actin filaments, imaging of micropipette-deformed red cell ghosts has allowed an ass

137 les with the cell adhesion area (for a given micropipette diameter and loading rate), which defines a

138 Since the micropipette diameter and the aspiration pressure are ou

139 usoidally displacing the cupula with a glass micropipette driven with a piezoelectric device while re

140 aspirating large unilamellar vesicles into a micropipette electrode, we are able to simultaneously mo

141 ement of solute concentrations by indwelling micropipette electrodes and the collection of perfusate

142 When micropipette electrodes containing NMDA receptor antagon

143 As determined in micropipette experiments, microvilli deform like an elas

144 atively comparable to experimental data from micropipette experiments.

145 information on cortical activity by means of micropipette experiments.

146 Using glass micropipettes, extracellular isolation of 37 interpolari

147 r in combination with estrogen through glass micropipettes fastened to the electrodes.

148 Electrical stimulation through a micropipette filled with DNA or other macromolecules ele

149 The patch clamp technique, in which a glass micropipette filled with electrolyte is inserted into a

150 s assessed by voltage clamp of oocytes using micropipettes filled with 2 M NaCl.

151 Here we describe a new technique, the micropipette force probe, that uses a micropipette as a

152 We present novel experiments in which two micropipette-held somatic cells of Volvox carteri, with

153 distorted cell, are visually demonstrated in micropipette-imposed deformation.

154 tactic accuracy and speed as they approach a micropipette in a manner that is dependent on the increa

155 Single vesicles were manipulated by micropipette in solutions of fluorescently labeled avidi

156 teries were isolated, removed and mounted on micropipettes in a sealed chamber.

157 cles of wild-type mice following perivenular micropipette injections of a control (LSIGRL) or PAR2-ac

158 Whole-cell K(+) currents recorded with a micropipette inserted into the cell soma were Ba(2+)-sen

159 mber is used, making it possible to insert a micropipette inside this chamber to hold a second bead b

160 ndard tool for IC recording, the patch-clamp micropipette is applied widely, yet remains limited in t

161 crospheres inside the lymphatic lumen with a micropipette is blocked by the lymphatic endothelium.

162 The micropipette is positioned perpendicular to the surface

163 cities of red cells when the pressure in the micropipettes is balanced.

164 f the initial lymphatic endothelium with the micropipette leads to rapid aspiration of intralymphatic

165 re used: Group I received a unilateral glass micropipette lesion into the vermal/paravermal region of

166 rigid micropores and in passive pulling into micropipettes, local compaction of chromatin is observed

167 zone), aggregometry (discrete interactions), micropipette manipulation (tether visualization), and ro

168 Micropipette manipulation measurements quantified the pr

169 Using micropipette manipulation of giant unilamellar vesicles,

170 n rat cremaster muscle was investigated with micropipette manipulation techniques.

171 Micropipette manipulation was used to expose a single li

172 o different methods, electron microscopy and micropipette manipulation, we have obtained two comparab

173 ilt, horizontal atomic force microscope with micropipette manipulation.

174 ptical-lever detection module with automated micropipette manipulation.

175 neutrophils or other amoeboid cells inside a micropipette, measurement of velocity versus counterpres

176 ly consistent with experimental results from micropipette measurements.

177 Binding to IgG-coated RBCs, measured using a micropipette method, indicated a 50-fold increase in eff

178 By using a micropipette method, we measured the kinetics of human F

179 vant presentation-using a recently developed micropipette method.

180 h juxtacellular Neurobiotin ejection, linked micropipette-microwire recording, and antidromic and ort

181 red microinjection system coupled to a glass micropipette needle.

182 Acetylcholine delivery from a micropipette onto a feed artery evokes hyperpolarisation

183 muli were produced by ejecting buffer from a micropipette onto the cell surface with a pneumatic pico

184 DEX droplets were formed either by manual micropipetting or within a continuous PEG phase by compu

185 robotic automation is difficult, however, as micropipette penetration induces tissue deformation, mov

186 e consistent with those determined using the micropipette perfusion and microdiffusion chamber techni

187 he use of the microdiffusion chamber and the micropipette perfusion technique, both of which have bee

188 al cortical injection of adenosine through a micropipette produced dose-dependent transient increases

189 The experiment was an extension of existing micropipette protocols.

190 Micropipette recording with juxtacellular Neurobiotin ej

191 ist-containing solution through a fine glass micropipette resulted in a spatially restricted increase

192 l protocol designed to approximately mimic a micropipette setting, we show that asymmetric incorporat

193 The integrated micropipette setup facilitates the easy manipulation and

194 Ideally, the micropipette should be as small as possible to increase

195 Aspiration of a cell and its nucleus into a micropipette shows that chromatin aligns and stretches p

196 Using a micropipette single cell adhesion assay able to measure

197 area expansion that is independent of either micropipette size or aspiration pressure.

198 r vesicles, each of which was aspirated in a micropipette so that we could monitor the tension of the

199 or Gi, a trimeric G protein) responded to a micropipette source of attractant by localizing RhoA act

200 External calibration curves were prepared by micropipetting standards with internal standard (IS) on

201 In contrast, Ag conjugated to the tip of a micropipette stimulates local, repetitive Ca(2+) puffs a

202 asuring piconewton-scale forces that employs micropipette suction is presented here.

203 With micropipette suction, we applied pulling forces to human

204 mitotic newt chromosomes was studied, using micropipette surgery and manipulation, for elongations u

205 n electrophysiologic approach-the servo-null micropipette system (SNMS)-for measuring hydrostatic pre

206 Arterioles were cannulated with a micropipette system and luminally pressurized.

207 electrophysiologic approach (the servo-null micropipette system, SNMS) that had been adapted for con

208 at the molecular level, we have developed a micropipette technique to quantify the redistribution of

209 Using a new micropipette technique, spherical, glassified protein mi

210 al cells from endothelial monolayers using a micropipette technique.

211 Myocytes were attached to micropipettes that extended from a force transducer and

212 ogenization, Triton-skinned, and attached to micropipettes that projected from a force transducer and

213 cell is caught and held between two suction micropipettes the surface membrane is destroyed by brief

214 he passive aspiration of a neutrophil into a micropipette, the active extension of a pseudopod by a n

215 WO tears were collected from 48 patients by micropipette, the WO sample after instillation of 10 muL

216 cause they are mounted on conventional glass micropipettes, the endoscopes readily fit standard instr

217 Evaporation is prevented by positioning the micropipette through a tiny hole in a cover glass, seale

218 was applied on the smaller end of a 1000 muL micropipette tip made of polypropylene.

219 s in a lipid bilayer formed at a patch-clamp micropipette tip under a buffer solution.

220 The micropipette tip was visualized in the anterior chamber.

221 , microiontophoresis of acetylcholine (1 mum micropipette tip, 1 muA, 500 ms) initiated dilatation th

222 Local delivery (1 microm micropipette tip; 500-2000 ms pulse) of acetylcholine (A

223 Multi-barreled glass-micropipettes (tip size 20-40 microm) were used to make

224 lls were biotinylated and manipulated with a micropipette to form an adhesive contact with a glass mi

225 affect neural activity in the AC, we used a micropipette to infuse salicylate (20 mul, 2.8 mM) into

226 noliters of whole blood, and only requires a micropipette to operate.

227 ure ejection or iontophoresis of ions from a micropipette to quantify diffusion characteristics of ne

228 utrophils and immobilized ICAM-1 while using micropipettes to control the force of contact between th

229 cular mechanoreceptors, TTX was applied with micropipettes to proximal segments of feed arteries, the

230 s, and the crawling of a neutrophil inside a micropipette toward a chemoattractant against a varying

231 lectrolyte that was suspended into two glass micropipettes using a conductive hydrogel.

232 Au nanoparticles (NPs) were coated on micropipettes using aminosilane linkers; and these micro

233 sensitivity to acid solutions, using the two-micropipette voltage clamp in Xenopus oocytes.

234 stress of fluid aspirated into a large-bore micropipette was then used to forcibly peel myotubes.

235 closed downstream, pressure (applied via the micropipette) was raised in a series of steps of 10 mmHg

236 By using a micropipette, we measured the conformational regulation

237 Fine-tipped (5 microm in diameter) micropipettes were advanced across the cornea with a pie

238 Stimulating micropipettes were inserted stereotaxically into the lat

239 For in vivo studies, micropipettes were used for recording and injecting vehi

240 ipettes using aminosilane linkers; and these micropipettes were used for stimulating and inhibiting t

241 Micropipettes were used to bring neutrophils into contac

242 urrent-clamp recordings with high-resistance micropipettes were used to characterize electrophysiolog

243 racellular recording techniques with 'sharp' micropipettes were used to evoke action potentials (APs)

244 Multibarrel glass micropipettes were used to record the activity of NST ne

245 th of the network projection pulled into the micropipette, where the network is strongly sheared in a

246 h the efficient continuous liquid feeding of micropipettes while allowing scalability to 1- and 2D pr

247 bility by whole cell patch using a multiplex micropipette with a common outlet to change artificial C

248 The DMB is a pulled glass micropipette with a fine tip that contains a microscopic

249 llisecond scale involves placing a recording micropipette with a membrane patch in front of a double-

250 The vessels were perfused via micropipettes with Ringer solutions containing bovine se