ライフサイエンスコーパス: microsequence

1 smallest dynactin subunit, was isolated and microsequenced.

2 lamide gel electrophoresis, and NH2-terminal microsequenced.

3 ansfer membrane, the 30,000 m.w. protein was microsequenced.

4 isolated from these libraries can be readily microsequenced.

5 zed p62 subunit of dynactin was isolated and microsequenced.

6 CBF2 activity was biochemically purified and microsequenced.

7 kinase 1 (CmCPK1), was cloned using peptide microsequences.

8 protein by affinity purification and peptide microsequencing.

9 s of three tryptic peptides were obtained by microsequencing.

10 ed, purified, and finally subjected to Edman microsequencing.

11 tides were analyzed by mass spectrometry and microsequencing.

12 nd protein kinases, as determined by protein microsequencing.

13 d through photoaffinity labeling and protein microsequencing.

14 ach band were analyzed by mass spectrometric microsequencing.

15 age, the purified proteins were suitable for microsequencing.

16 fied by gel electrophoresis and subjected to microsequencing.

17 s of the Sec6-Sec8-Sec15 complex for peptide microsequencing.

18 ng over 70 amino acids identified by peptide microsequencing.

19 ]halothane, and subjected to proteolysis and microsequencing.

20 e first 10 amino acids determined by protein microsequencing.

21 should be compatible with both MALDI-TOF and microsequencing.

22 (35)S-labeled C2GnT-M was established using microsequencing.

23 ure liquid chromatography and amino-terminal microsequencing.

24 een isolated and subjected to amino-terminal microsequencing.

25 ed from the retina, was confirmed by protein microsequencing.

26 be authentic betaB(2)-crystallin by protein microsequencing.

27 ere identified by phosphopeptide mapping and microsequencing.

28 acting proteins by affinity purification and microsequencing.

29 f three N-terminal residues, as confirmed by microsequencing.

30 Electrophoresis and microsequencing allowed us to identify 20 proteins.

31 mor cell lines prescreened for probable LOH, microsequence alterations of MMAC1 were detected in 12 (

32 nding protein by far-Western blotting and by microsequence analyses of a 34-kDa protein purified by E

33 Microsequence analyses of delta demonstrate that this li

34 Microsequence analyses of H3 from Tetrahymena, yeast, an

35 SDS-PAGE and microsequence analyses revealed that the Mr 64,000 fgl2

36 The 35-kDa protein was identified by peptide microsequence analysis as the human thioesterase II (hTE

37 approach facilitates sample preparation for microsequence analysis at low picomole level.

38 Peptide microsequence analysis by capillary high-performance liq

39 Microsequence analysis obtained after limited proteolysi

40 e protein which was originally identified by microsequence analysis of a 67.8 kDa protein spot (pI 5.

41 Microsequence analysis of LCRF yielded an amino acid seq

42 Microsequence analysis of peptides derived from the 100-

43 Microsequence analysis of the E1B2 antigen revealed that

44 Affinity purification and microsequence analysis of the proteins in the doublet id

45 Automated microsequence analysis of the purified enzymes establish

46 Edman microsequence analysis of the subunits after import perm

47 Microsequence analysis of this approximately 80-kDa poly

48 Microsequence analysis of two polypeptide components of

49 ors from HeLa nuclear extract and amino acid microsequence analysis performed.

50 Microsequence analysis revealed that OE-1 recognized hum

51 Purification, microsequence analysis, and cross-blotting experiments i

52 10 protein was gel isolated and subjected to microsequence analysis, and the gene was cloned.

53 The primary structure was determined by microsequence analysis, mass spectrometry, and C-termina

54 hase-high performance liquid chromatography, microsequence analysis, mass spectrometry, and immunocyt

55 axima (pumpkin) phloem exudate and, based on microsequence analysis, the cDNA encoding CmPS-1 was clo

56 This identification, initially made by microsequence analysis, was verified by showing that (i)

57 that mimics the effector domain of Rab3A and microsequence analysis, we found calmodulin to be a majo

58 H and the 125-kDa glycoprotein to amino acid microsequence analysis.

59 fic components were identified using peptide microsequence analysis.

60 Through microsequencing analysis and chromatin immunoprecipitati

61 ermination, endoglycosidase sensitivity, and microsequencing analysis as a porcine homologue of CD58.

62 In this study, microsequencing analysis of the radiolabeled downstream

63 N-terminal and C-terminal microsequencing analysis showed the expected unique term

64 s band was subsequently shown by Western and microsequencing analysis to comprise both the carboxylas

65 terminal amino acid sequence was obtained by microsequencing analysis, and full-length clones were ob

66 By immunoprecipitation and protein microsequencing analysis, we have identified a major cel

67 tide mapping, site-directed mutagenesis, and microsequencing analysis, we identified two major sites

68 ctive protein spot (MW: 44 kDa, pI: 4.5) was microsequenced and the related cDNA was cloned yielding

69 the protein was revealed by automated Edman microsequencing and a computer database search.

70 Peptide microsequencing and confirmatory mutagenesis identified

71 cleavage sites were identified by N-terminal microsequencing and electrospray mass spectrometry, and

72 Microsequencing and immunoblot analysis revealed that th

73 Analysis of the phosphopeptides by automated microsequencing and manual Edman degradation identified

74 hoto-cross-linking in TnC were determined by microsequencing and mass spectrometry following enzymati

75 ross-linking site for both TnC mutants using microsequencing and mass spectrometry following proteoly

76 Carcinus maenas, was determined by automated microsequencing and MS, and was almost identical to that

77 Protein microsequencing and peptide mapping of wild-type and mut

78 itory protein that was identified by peptide microsequencing and protein database analysis as troponi

79 of the pollen oleosins can be identified by microsequencing and specific immunoblotting.

80 Following N-terminal microsequencing and subsequent isolation of the correspo

81 PY proteins identified by microsequencing and Western blotting include Cbl, STAT3,

82 s a mixture of alpha- and beta-synucleins by microsequencing and Western blotting.

83 affinity chromatography and characterized by microsequencing and Western blotting.

84 bioactive and intermediate CART molecules by microsequencing and/or high performance liquid chromatog

85 re able to serendipitously purify, partially microsequence, and clone human copine III.

86 n-regulated proteins, FegA, was purified and microsequenced, and a reverse genetics approach was used

87 ne of these spots at pH 5.7 was subsequently microsequenced, and five partial amino acid sequences we

88 e methylesterase-1 (PME-1), was purified and microsequenced, and its cDNA was cloned.

89 amino terminus of cauliflower RPB5 that was microsequenced, and shows 42 and 53% amino acid sequence

90 The purified protein was microsequenced, and the sequence information was used to

91 Both proteins were identified by peptide microsequencing, and a complementary DNA encoding the hi

92 ization tandem mass spectrometry, N-terminal microsequencing, and immunoblotting with specific antibo

93 elution with the BIR3 binding peptide AVPIA, microsequencing, and mass spectrometry.

94 Limited proteolysis, microsequencing, and sedimentation analyses indicate tha

95 in was purified and identified by N-terminal microsequencing as heat shock protein 60 (Hsp60).

96 eins labeled in this assay was identified by microsequencing as HSP25, purified as a recombinant prot

97 using 2-dimensional gels and amino-terminal microsequencing as one of a select few [35S]methionine p

98 and its identification by mass spectrometry microsequencing as pregnancy-associated plasma protein-A

99 To this end we purified and identified via microsequencing ATF2 as a major URE- bound and CREB-asso

100 the distribution and subsequent targeting of microsequences by the mutation mechanism.

101 Microsequencing by mass spectroscopy and Western blot an

102 s were identified from tryptic digests using microsequencing by tandem mass spectrometry and database

103 Structures were established by NH2-terminal microsequencing, cross-link analysis, electrospray mass

104 Microsequence data from phloem-purified 31 kDa protein w

105 On the basis of protein microsequence data, we conclude that the synthesis of th

106 s, direct [(14)C]halothane photolabeling and microsequencing demonstrated dominant labeling of W9, le

107 subunits on analytical SDS-PAGE, and protein microsequencing establishes that the GABA(A)R-modulating

108 Purified LRBP was subjected to N-terminal microsequencing followed by homology search, which revea

109 The results obtained from fragmented protein microsequencing have suggested that autocrine motility f

110 novel mass spectrometry strategy and peptide microsequencing identified 33 known proteins, many of wh

111 Protein microsequencing identified one of the SH3-binding protei

112 Protein microsequencing identified the 90-kDa protein as mitocho

113 Purification and partial microsequencing identified the protein as an adenine nuc

114 Microsequencing identified this protein as the glycolyti

115 Peptide microsequencing identified this protein as the product o

116 Microsequencing identified this protein as vasodilator-s

117 Microsequencing identified three serine residues, each o

118 The results of microsequencing, immunochemical analysis of 2D-PAGE blot

119 rmined using epitope-defined antibodies, and microsequencing indicated that K18 cleavage occurs at a

120 Immunoaffinity purification and microsequencing indicated that the core peptide of the S

121 ry analysis of peptide fragments and peptide microsequencing, indicates that Cdc37 is comprised of th

122 ve the following primary structures by Edman microsequencing: IWLTALKFLGKHAAKHLAKQQLSKL-NH2 for lycot

123 e VP16 activation domain followed by peptide microsequencing led to the identification of ARC92 as a

124 It is highly desirable to have microsequencing methods that can be used to precisely id

125 re purified to homogeneity as ascertained by microsequencing of 14-17 N-terminal amino acids.

126 Microsequencing of a 17-amino acid peptide of this polyp

127 Microsequencing of a p180 peptide revealed 92% identity

128 protein, caveolin-2, was identified through microsequencing of adipocyte-derived caveolin-enriched m

129 ify of the purified protein was confirmed by microsequencing of an endoproteinase glutamic acid-C fra

130 Microsequencing of an internal peptide derived from puri

131 By using mass spectroscopy and direct microsequencing of CNBr fragments of phospho-moesin, the

132 Microsequencing of cyanogen bromide fragments of purifie

133 Microsequencing of DIPP revealed a 'MutT' domain, which

134 Microsequencing of five peptides derived from purified p

135 Protein microsequencing of fragments isolated from proteolytic d

136 Microsequencing of its two tryptic peptides revealed two

137 Information gained from microsequencing of oligopeptides obtained by tryptic dig

138 Amino acid microsequencing of one of these proteins led to the iden

139 Microsequencing of partially purified p18 identified it

140 Microsequencing of peptide fragments from a cyanogen bro

141 Microsequencing of peptides derived from a 50-kDa paxill

142 Microsequencing of peptides derived from purified rat pp

143 Microsequencing of Src-phosphorylated caveolin revealed

144 Microsequencing of the 50-kDa band yielded an amino-term

145 Furthermore, microsequencing of the 95 kDa protein yielded 13 peptide

146 Microsequencing of the isolated peptide-beads revealed t

147 Microsequencing of the most prominent COM crystal-bindin

148 d sequences with those obtained from peptide microsequencing of the purified complex components.

149 Purification and microsequencing of the strongest band (88 kDa) demonstra

150 n (KuAg) as documented by partial amino acid microsequencing of tryptic digests and immunologic react

151 Microsequencing of tryptic peptides from the two protein

152 Amino acid microsequencing of two cyanogen bromide-generated peptid

153 hich were successfully identified by protein microsequencing or matrix-assisted laser desorption ioni

154 tic oligopeptide that was not represented in microsequenced peptides of the trans-factor) also recogn

155 Microsequencing proved that this protein is the lymphocy

156 Microsequencing provided unique peptide sequences of app

157 Consistent with the microsequencing results pMS100 failed to complement a tr

158 unoaffinity purification of this protein and microsequencing revealed homology to sheep CD58 as well

159 Peptide microsequencing revealed that p140 is probably identical

160 Protein microsequencing revealed that R-[(3)H]mTFD-MPAB did not

161 Microsequencing revealed that the 47 kDa BAF is identica

162 Peptide microsequencing revealed that the enriched protein was E

163 Peptide microsequencing revealed that the purified protein was v

164 ize exclusion chromatography, and subsequent microsequencing revealed that they are the zeta isoform

165 These polypeptides were purified and microsequenced, revealing that p58/53 is identical to th

166 beads from the library and subjected them to microsequencing, revealing the sequence of the unreacted

167 Protein microsequencing reveals that one constituent of this com

168 Microsequencing reveals that protease-accessible regions

169 Microsequencing showed it to be human endonuclease G, an

170 The sequence was confirmed by microsequencing six peptides of mature REJ and by Wester

171 We have compared the microsequence specificity of mutations introduced during

172 Microsequencing studies identified the GM35 Ag as human

173 ing of Nef function, we used biochemical and microsequencing techniques to isolate and identify Nef-a

174 ain their amino acid sequences using protein microsequencing techniques.

175 we demonstrated by protein purification and microsequencing that ribosomes of maize (Zea mays L.) co

176 We demonstrated by immunoblotting and microsequencing that the EspD protein is secreted via th

177 minus was determined for the beta-subunit by microsequencing the protein purified from etiolated maiz

178 liquid chromatography, together with protein microsequencing, the protein was identified as heregulin

179 igen was purified by immunoprecipitation and microsequenced; the peptides sequenced showed 100% homol

180 S-4 from human embryonic kidney 293 cells by microsequencing through mass spectrometry.

181 rain) G2 and G1 proteins were established by microsequencing to be equivalent to aa 525 and 1046, res

182 lypeptides (12, 32 and 38 kDa) were found by microsequencing to be identical to parts of the predicte

183 ely with two polypeptides that were shown by microsequencing to be the alpha- and beta-subunits of Ac

184 romatography and SDS-PAGE and found by using microsequencing to be the protein flagellin.

185 olypeptide was purified and shown by peptide microsequencing to be the Torpedo ortholog of the small

186 esent in this construct and was subjected to microsequencing to confirm its identity and the site of

187 It is now routine using automatic Edman microsequencing to determine the primary structure of pe

188 his particle and performed mass spectrometry microsequencing to determine their identities.

189 ach of which was subjected to the N-terminal microsequencing to identify the cleavage sites.

190 l I-like ectodomain followed by SDS-PAGE and microsequencing using electrospray (ISI-Q-TOF-Micromass)

191 N-terminal microsequencing was performed to simultaneously confirm

192 nous moesin, which was identified by peptide microsequencing, was dependent on phosphatidylglycerol (

193 ed by [(3)H]AziPm were identified by protein microsequencing, we found propofol-inhibitable photolabe

194 ombination of mutational analysis and direct microsequencing, we have determined that this cysteine p

195 chromatographic purification and subsequent microsequencing, we have identified ERSF as TFII-I.

196 by tandem mass spectrometry (TMS), and novel microsequences were identified that indicated a previous

197 protein was subjected to tryptic mapping and microsequencing, which was followed by molecular cloning