ライフサイエンスコーパス: microtiter

1 Microtiter 96-well and three-dimensional cell-based micr

2 n biofilm initiation, as confirmed by static microtiter and continuous culture flow cell and tubing b

3 The microtiter array including IID sensors 1 and 2 is capabl

4 Here, the characterization of sensors and microtiter arrays comprising the IIDA are reported.

5 A microtiter assay was developed for the improved detectio

6 AMP reaction, and a quantitative cohemolysis microtiter assay was developed using purified sphingomye

7 , the level of biofilm formation in a static microtiter assay was diminished in T2S mutants.

8 Using a plasma-coated microtiter assay, biofilm formation was restored to the

9 For the microtiter assay, either spent bacterial culture superna

10 d in a modified version of the 96-well plate microtiter assay.

11 A similar strategy may be used to develop microtiter assays for many further DNA modifying enzymes

12 A microtiter-based assay system is described in which DNA

13 We employed this microtiter-based assay to determine the antifungal susce

14 wo Petri dishes connected with tubing; and a microtiter-based assay.

15 A simple, rapid, and reproducible microtiter-based chondroitinase (CSase) assay is reporte

16 ribe a rapid and highly reproducible 96-well microtiter-based method for the formation of fungal biof

17 In contrast with microtiter-based screening, this system does not involve

18 cribed in this paper, waveguides, analytical microtiter chambers and fluidic capillary channels, with

19 lectrodes integrated within ultra-low-volume microtiter chambers for the amperometric determination o

20 ay, using waveguides to address miniaturized microtiter chambers with volumes of 230 and 570 pL.

21 s is a versatile method that can be used for microtiter determinations of biofilm antimicrobial susce

22 tibility to chlorhexidine gluconate (CHG) by microtiter dilution; mupirocin susceptibility was tested

23 The microtiter dish assay is a more expedient method for mea

24 We report here the development of a 96-well microtiter dish assay.

25 oughput sample analysis of APR activity in a microtiter dish format.

26 iene C(4) (LTC(4)) was measured in a 96-well microtiter dish with rapid filtration onto a Perkin Elme

27 ly monitor proteolytic activity in a 96-well microtiter fluorescent plate assay.

28 In an earlier report, we optimized a microtiter format assay system to screen potential bioac

29 The microtiter format assay was validated by its sensitivity

30 Additionally, in the microtiter format, multiple sets of kinetic experiments

31 amster ovary cells, in both microfluidic and microtiter formats.

32 by subsequent optimization through iterative microtiter library synthesis followed by in situ activit

33 Testing was performed in frozen microtiter panels according to the Clinical and Laborato

34 lts were compared to those of a colorimetric microtiter PCR enzyme immunoassay (PCR-EIA) used as a di

35 novel compounds at nominal levels of 10mM in microtiter plate (MTP) format.

36 A 96- or 24-well microtiter plate (MTP) was positioned on the gadget's sc

37 d covalently attached to a polystyrene based-microtiter plate (MTP), pretreated with KOH.

38 1:1 (v/v) and dispensed in a KOH-pretreated microtiter plate (MTP).

39 ween GCL activities measured by HPLC and NDA-microtiter plate analyses.

40 he FP assay has been formatted in a 384-well microtiter plate and automated using a pipeting workstat

41 ample preparation device has the format of a microtiter plate and is molded in a plastic frame which

42 f APPmicroTP is also checked with respect to microtiter plate and paper-based ELISA.

43 rough wash device was positioned between the microtiter plate and the ESI interface.

44 pho-ERK, and the signals in the wells of the microtiter plate are quantified by a LI-COR infrared sca

45 els of cytochrome c contained in a 1536-well microtiter plate are shown.

46 print at > x 10 higher density on a standard microtiter plate area than currently possible, our cell

47 introduction into cells by transfection in a microtiter plate array.

48 A microtiter plate assay based on the original quartz cuve

49 onstrate the accuracy and versatility of the microtiter plate assay by application to the kinetic ana

50 An automated electrochemical microtiter plate assay for the quantification of free ra

51 A microtiter plate assay format permitted a rapid determin

52 le and cost-effective nanoparticle method as microtiter plate assay format shows great potential for

53 tylation but does not inhibit trypsin, and a microtiter plate assay of the SIRTs has been devised usi

54 ystems, this concept is applied to develop a microtiter plate assay to detect biotin (as a model for

55 throughput methyl viologen-based photometric microtiter plate assay was established to determine the

56 Biofilm formation was measured by using a microtiter plate assay with the crystal violet staining

57 We have developed and optimized a 96-well microtiter plate assay, based on the reduction of alamar

58 say systems, surface plasmon resonance and a microtiter plate assay, demonstrated that the beta3 A-do

59 creened for biofilm-negative mutants using a microtiter plate assay.

60 antifungal activity were determined using a microtiter plate assay.

61 es of avidin and horseradish peroxidase in a microtiter plate assay.

62 n of CTB produced by V. cholerae in a simple microtiter plate assay.

63 matographic column fractionation followed by microtiter plate assay.

64 ng abilities of the swarming mutants using a microtiter plate assay.

65 ropneumoniae serotype 5 itself, in a 96-well microtiter plate assay.

66 or biofilm formation by E. faecalis, we used microtiter plate assays and a chemostat-based biofilm fe

67 g beads accelerate amyloid fibrillization in microtiter plate assays are unclear.

68 levels were determined by standardized ELISA microtiter plate assays from a commercial (bFGF) or prop

69 bes both thin-layer chromatography (TLC) and microtiter plate assays, which use bioluminescence or th

70 produces comparable results to conventional microtiter plate assays, yet requires 2 orders of magnit

71 s, NMR, isothermal titration calorimetry and microtiter plate assays.

72 405, and mixtures of the two organisms using microtiter plate assays.

73 to bovine submaxillary mucin in solid-phase microtiter plate assays.

74 d total assay time 2 fold in comparison with microtiter plate assays.

75 antibodies and applied as a novel label in a microtiter plate based immunoassay and a quantitative co

76 Further two-hybrid analysis and microtiter plate binding assays localized the sites of i

77 mbiae BBMV and bound Cry11Ba on dot blot and microtiter plate binding assays with a calculated K d of

78 the MMAC1/ PTEN-PDZBD peptide inhibited both microtiter plate binding to the hDLG and hMAST205 PDZ do

79 Microtiter plate biofilm analyses indicated that biofilm

80 ge as the conventional method on the 96-well microtiter plate but has advantages such as less reagent

81 A single 96-well microtiter plate can analyze approximately 20 specimens,

82 For directed GOx evolution a microtiter plate detection system based on the quinone d

83 tive extraction were tested against standard microtiter plate ELISA.

84 In both a solution phase and a microtiter plate elongation assay, IAPP fibrils are poor

85 ed in a single well using a standard 96-well microtiter plate enzyme-linked immunosorbent assay (ELIS

86 640 compounds in the footprint of a standard microtiter plate for the identification of novel agonist

87 p10 manual pipette, or in a fully automated microtiter plate format (96 samples at a time) employing

88 The assay is carried out in a microtiter plate format and measures cellular proliferat

89 ion fluorescence activity assay to a 96-well microtiter plate format and uses a plate reader to detec

90 The assay performed in a 96-well microtiter plate format employs biotin-labeled gelatin (

91 The nanoparticles were used in a microtiter plate format for glycopeptide capture using a

92 d has been adapted to a 384-well, low-volume microtiter plate format that can be used for the high-th

93 and the well-known ABTS-assay was applied in microtiter plate format to validate oxygen independency

94 onstrate the utility of this PETE assay in a microtiter plate format using the RNA-dependent RNA poly

95 gurations, we describe its use in a 96-well, microtiter plate format with a lower plate containing H2

96 tration determinations and was scaled to the microtiter plate format with appropriate mixing, dispens

97 hnologies for quantification of compounds in microtiter plate format without the need for authentic c

98 ower than 10%), and high throughput (96-well microtiter plate format) make this assay an excellent su

99 ODIPY-FL-casein assay is easily adapted to a microtiter plate format, so it can be used to screen lar

100 al model reactions which are compatible with microtiter plate format, such as monitoring enzymatic re

101 25I-labeled RIA ([125I] RIA) is adapted to a microtiter plate format, termed mini-RIA.

102 say was readily adapted for use in a 96-well microtiter plate format.

103 he reaction wells containing the probes in a microtiter plate format.

104 sponse curve for the analyte in a convenient microtiter plate format.

105 asure poly(A) polymerase (PAP) activity in a microtiter plate format.

106 rization of (33)P-rEBP and rEBP in a 96-well microtiter plate format.

107 ermination of PEG molecular weight (MW) in a microtiter plate format.

108 ors in the presence of only 10 nM sirtuin in microtiter plate format.

109 as a ready-to-go assay was prepared for the microtiter plate format.

110 Rubidium flux was performed in a 96-well microtiter plate format; rubidium was quantified using a

111 RBP4 in high-density 384-well and 1536-well microtiter plate formats.

112 d robust in nature, and also compatible with microtiter plate formats.

113 e protocol is applicable to 96- and 384-well microtiter plate formats; uses a commercially available

114 The microtiter plate has been an essential tool for diagnost

115 ystem, the total analysis time for a 96-well microtiter plate has been reduced to approximately 5 min

116 ed as biolabels in quantitative fluorescence microtiter plate immunoassay and qualitative on-site col

117 We have developed a microtiter plate immunoassay for counting single molecul

118 l in wheat and maize samples by fluorescence microtiter plate immunoassay with an IC50 of 220 mug kg(

119 as several nanomoles can be determined in a microtiter plate in 10-20 min.

120 or a fluorescein attached to the wells of a microtiter plate in a competitive fashion.

121 Real time PCR (RT-PCR) in a microtiter plate is the preferred method for quantitativ

122 EK Diagnostic Systems, Cleveland, OH) uses a microtiter plate MIC format for susceptibility testing o

123 In addition to a qualitative colorimetric microtiter plate PCR assay (MTP-PCR) and a semiquantitat

124 Use of a 96-well microtiter plate permits automated and sensitive quantif

125 be similar to those generated using a manual microtiter plate procedure.

126 was attained for the on-cell assay using the microtiter plate reader approach, whereas as low as 2 mi

127 The device is then placed into a standard microtiter plate reader for measurement, with the entire

128 leased from mammalian cells using a standard microtiter plate reader to measure wells integrated into

129 ntracellular C1-BODIPY-C12 fluorescence on a microtiter plate reader, whereas extracellular fluoresce

130 croscope and quantification after lysis in a microtiter plate reader.

131 ligation and detection using a fluorescence microtiter plate reader.

132 bda(ex)=379 nm and lambda(em)=513 nm using a microtiter plate reader.

133 ws measurements of the reactions by standard microtiter plate readers without any additional steps in

134 ity is incompatible with enhanced throughput microtiter plate screening.

135 the results obtained with a PCR colorimetric microtiter plate system by use of clinical CSF specimens

136 the integration of these arrays with 96-well microtiter plate technology to create microarrays in mic

137 uent into a system that can be combined with microtiter plate technology.

138 phase approach, SPEG can be performed with a microtiter plate to provide a high-throughput platform f

139 The AK assay is performed in a single microtiter plate using an 'add and read' procedure that

140 The microtiter plate was placed vertically on a three-dimens

141 ured by one set of antibodies immobilized in microtiter plate wells and detected using a second antib

142 When HDMVEC were cultured in microtiter plate wells coated with concentrations of C1q

143 immunoassay was performed in antigen-coated microtiter plate wells for simultaneous qualitative and

144 Microtiter plate wells modified with thin (approximately

145 Capture DNA is conjugated to the surface of microtiter plate wells through a biotin-streptavidin int

146 es can be predried to the assay vials, e.g., microtiter plate wells, and readout is ready in 10 min a

147 proteins, but not fibronectin-coated plastic microtiter plate wells, was specifically blocked by anti

148 us to monitor protein unfolding in a 96-well microtiter plate with a UV plate reader.

149 P can quantify an analyte as precisely as in microtiter plate with insignificant non-specific binding

150 by functionalizing a KOH-treated polystyrene microtiter plate with multiwalled carbon nanotubes (MWCN

151 e introduction from a commercially available microtiter plate without the need for a separate fluid d

152 proximately 17 pCi/ well (or 1.6 nCi/96-well microtiter plate) 14C-1A5 was used, which is far below t

153 e small stationary volumes (e.g., wells of a microtiter plate) or flowing volumes of liquids (e.g., m

154 means for accessing samples serially from a microtiter plate, channels for assembling eight parallel

155 FP reporter cells were seeded into a 96-well microtiter plate, incubated for 24 h, and then treated w

156 As it is based on a standard 96-well, microtiter plate, it is amenable to automation and high

157 ith the convenience and standardization of a microtiter plate, make arrays of SAMs a versatile tool t

158 Our platform is an ideal alternative to the microtiter plate, works with different volumes, is compa

159 Here we describe a fluorescently detected microtiter plate-based assay for inhibitor binding to HM

160 In the present study, we describe a microtiter plate-based assay for quantitation of the amo

161 acroglobulin capture to develop a sensitive, microtiter plate-based assay for serum chymase, assisted

162 using either a thin film biosensor chip or a microtiter plate-based assay.

163 A new format for the microtiter plate-based assays was proposed.

164 ing nitrocellulose-based CBM macroarrays and microtiter plate-based CBM capture and competitive-inhib

165 or maltose using amylose magnetic beads in a microtiter plate-based format.

166 ed and optimized methodology for the 96-well microtiter plate-based measurement of Histoplasma yeast

167 so demonstrated using this relatively simple microtiter plate-based polycation detection system.

168 Microtiter plate-based robotic electrochemical antioxida

169 Microtiter plate-binding assays, coprecipitation experim

170 n an area equivalent to a standard well of a microtiter plate.

171 erformed simultaneously in single wells of a microtiter plate.

172 nventional 9-mm pitch (spacing) of a 96-well microtiter plate.

173 rformed on a micromolar scale in situ in the microtiter plate.

174 ates on gold that are presented on a 96-well microtiter plate.

175 tion of both genotypes in a single well of a microtiter plate.

176 a reader device with the same footprint as a microtiter plate.

177 anglioside (GM1) was used for coating of the microtiter plate.

178 ation of components in the sample wells of a microtiter plate.

179 precipitation to be carried out in a single microtiter plate.

180 implicity of CiGiP with the convenience of a microtiter plate.

181 lly through the 24 vials of a standard 6 x 4 microtiter plate.

182 Milk also removes ricin bound to the microtiter plate.

183 or copolymer are added to wells of a 96-well microtiter plate.

184 ISA) method in which Hb was immobilized on a microtiter plate.

185 d and cost-effective cellphone-based 96-well microtiter-plate (MTP) reader, capable of performing AST

186 ensitivity level reasonably close to that of microtiter-plate Europium nanoparticle assay.

187 We developed a 96-well microtiter-plate high-throughput screening (HTS) assay f

188 itopes markedly changed between the free and microtiter-plate immobilized state as revealed by antibo

189 oped a sensitive Europium nanoparticle-based microtiter-plate immunoassay capable of detecting target

190 sity of which can then be determined using a microtiter-plate reader.

191 r to subsequent merging of microfluidics and microtiter-plate technology for high-throughput assessme

192 amperometric sensing of glucose solutions in microtiter-plate wells used computer-controlled stepper

193 tion and application of a spectrophotometric microtiter-plate-based assay for NAAAR.

194 lane-functionalized 96-well chemiluminescent microtiter plates (MTP) using 1-ethyl-3-(3-dimethylamino

195 es coating fibroblast growth factor (FGF) on microtiter plates and capturing fluorescein isothiocyana

196 f the obtained liposomes with the surface of microtiter plates and cartridges were investigated and 3

197 Because immobilization of proteins on microtiter plates and exposure of immobilized proteins t

198 nity for light chains immobilized on 96-well microtiter plates and for beads conjugated with a light

199 lbicans strain SC5314 were formed in 96-well microtiter plates and on silicon elastomer pieces using

200 amenable to application in 96- and 384-well microtiter plates and should prove useful for high-throu

201 carbohydrates (6-16) were also displayed in microtiter plates and successfully screened with various

202 Immortalized human cells are grown in microtiter plates and treated with compounds from a smal

203 ing protein (PBP) constructs by compounds in microtiter plates by means of competition with time-depe

204 re of the trkA receptor from cell lysates in microtiter plates coated with an anti-trk antibody.

205 In ELISA, microtiter plates coated with zymosan induced efficient

206 s formed on peg lids can then be fitted into microtiter plates containing test agents.

207 d digestion and peptide elution into 96-well microtiter plates followed by LC-MS analysis.

208 say that can be conveniently performed using microtiter plates for the discovery and/or validation of

209 These devices illuminate samples in microtiter plates from one side and use the RGB-based im

210 nolates is widely used and a desulphation in microtiter plates has been applied to reach high through

211 erformed unattended runs of up to 17 96-well microtiter plates in 8h was constructed.

212 d the DropArray technology with conventional microtiter plates in a cell-based protein-binding assay.

213 o collagens type I, II, and IV adsorbed onto microtiter plates in a dose-dependent saturable manner.

214 an also be printed into 96-well glass bottom microtiter plates in a multiplexed manner, and the fluor

215 time, show that genes that are close on the microtiter plates in microarray experiments also tend to

216 gmentation chain transfer) polymerization in microtiter plates in the open atmosphere.

217 e of a single-tube reaction in ligand-coated microtiter plates indicates the versatility of PRIME dis

218 standard STREP-HRP colorimetric reaction in microtiter plates of differing optical quality produced

219 e enables combinatorial polymer synthesis in microtiter plates on the benchtop without the need of hi

220 gnificant reductions in biofilm formation on microtiter plates or hydroxylapatite disks.

221 pecific capture probes were immobilized onto microtiter plates or silicon chips.

222 ridoma cells, as well as clonal expansion in microtiter plates over several weeks, and the number of

223 adily multiplexed and scaled up in multiwell microtiter plates to allow simultaneous parallel detecti

224 ed antiglobulin bound to streptavidin-coated microtiter plates to immobilize antiphospholipase C-gamm

225 s only 100 ng of input RNA and uses standard microtiter plates to process samples in parallel.

226 of acapsular S. aureus to fibrinogen-coated microtiter plates was enhanced.

227 ts of custom-made frozen 96-well polystyrene microtiter plates were used and prepared with 2x predilu

228 The HNO-sensing microtiter plates were used to quantify pH-dependent HNO

229 Here, we show that simultaneous treatment of microtiter plates with chitosan, a deacetylated form of

230 yme A reductase (HMGR) inhibitors in 96-well microtiter plates with rapid workup using established ma

231 By co-coating microtiter plates with SNAP25 substrate and a monoclonal

232 Precoating of microtiter plates with two species of oxidized A2E, pero

233 mpounds against aminopeptidase M in 384-well microtiter plates with Z factors ranging from 0.53 to 0.

234 f 96-well or 384-well density PPSF-resistant microtiter plates without the requirement for multiple o

235 nd titration, (v) dispensing of embryos into microtiter plates, and (vi) reporter quantification.

236 d FACS-sorted cells were added per well into microtiter plates, and after 11 days at 37 degrees C the

237 ction domains in individual wells of 96-well microtiter plates, and for quantifying domain-peptide in

238 Using pre-coated and blocked microtiter plates, and pre-prepared liposome reagents, a

239 omain alone, increased initial attachment to microtiter plates, as did in trans expression of the A d

240 In microtiter plates, biotinylated high molecular weight ki

241 Binding assays performed in microtiter plates, by two-dimensional (2D) Western blott

242 ed 450 high-confidence interactions using 47 microtiter plates, versus thousands of plates expected u

243 aphic resins aliquotted in membrane-bottomed microtiter plates.

244 b, IgG, amyloid beta, and BSA immobilized on microtiter plates.

245 ntifying CTB bound on epithelial surfaces in microtiter plates.

246 ed in rabbit plasma and on fibrinogen-coated microtiter plates.

247 analogs in assays performed in 100,000-well microtiter plates.

248 ied out in liquid medium in standard 96-well microtiter plates.

249 of in vitro biofilm formation on polystyrene microtiter plates.

250 cycloheximide-treated HEp-2 cells in 96-well microtiter plates.

251 e pools of fosmid clones arrayed in 384-well microtiter plates.

252 ped for high-throughput screening in 96-well microtiter plates.

253 ed hybrid is captured on streptavidin-coated microtiter plates.

254 ploying native enzyme that is immobilized on microtiter plates.

255 erminal regions of both proteins in 384-well microtiter plates.

256 to high molecular weight kininogen linked to microtiter plates.

257 which functioned as a receptacle for 96-well microtiter plates.

258 l binding to immobilized ligand using V-well microtiter plates.

259 ays running at low sirtuin concentrations in microtiter plates.

260 compared with static protocols performed in microtiter plates.

261 borosilicate glass surfaces and polystyrene microtiter plates.

262 t glycosidases (cellulases and xylanases) in microtiter plates.

263 of an appropriate desulphation procedure in microtiter plates.

264 ommended for high throughput desulphation in microtiter plates.

265 ubculturing visibly clear wells from the MIC microtiter plates.

266 Microtiter proliferation assays revealed that the Tax-1

267 sub-second positioning of any location on a microtiter-sized target with up to 1536 samples with mic

268 ility of this glycosaminoglycan to adhere to microtiter surfaces.

269 I guidelines using a custom, broth dilution, microtiter system.

270 ence of treated versus untreated polystyrene microtiter trays on caspofungin MICs using 209 isolates

271 sites found nonspecifically in conventional microtiter well assays.

272 describe simple screens using red cells in a microtiter well format to identify intercalating agents

273 mmobilize the enzyme on a solid surface in a microtiter well format.

274 occus aureus biofilms was investigated using Microtiter Well Plates (MWP) and Drip Flow Reactors (DFR

275 Chitin-coated microtiter well plates captured a CBD-tagged heterodimer

276 e, similar in size and pitch to conventional microtiter well plates, but offer electrodes along the i

277 sable pipet tips and conventional disposable microtiter well plates.

278 carbon chain that noncovalently binds to the microtiter well surface.

279 and 200 eukaryotic cells were measured in a microtiter well under the optimized conditions.

280 t hearts; cells were plated (5 x 10(4) cells/microtiter well) and challenged with either vehicle or n

281 PSA antibody immobilized to the surface of a microtiter well, (2) PSA, and (3) an anti-PSA antibody-U

282 ome this problem, we designed an alternative microtiter well-based binding assay in which Gluc is cap

283 poB) was measured by capturing h-apoB-100 in microtiter wells and detecting OxPL by antibody E06.

284 and were defective in pellicle formation in microtiter wells and in a biofilm attachment assay.

285 1 but only when the beta(2)GP1 was plated on microtiter wells coated with OxCL.

286 )beta(3) bound in a dose-dependent manner to microtiter wells coated with the isolated 190-kDa proteo

287 eterize the unique features occurring within microtiter wells containing specific growth media source

288 In microtiter wells factor XI binds to more sites in the ab

289 ssive noncovalent adsorption of heparin onto microtiter wells following their treatment by plasma pol

290 n serumless medium adhere and spread well on microtiter wells in the presence of fetuin and divalent

291 enterica grown either in polyvinyl chloride microtiter wells or in urethral catheters.

292 Isolates formed biofilms in microtiter wells to various degrees.

293 ormat onto the bottom of streptavidin-coated microtiter wells.

294 VCAM-1 and 7d-VCAM-1 coated onto polystyrene microtiter wells.

295 tachment of the tested strain to polystyrene microtiter wells.

296 (B)) necessary for coupling to avidin coated microtiter wells.

297 ed cells are added to ligand-coated V-shaped microtiter wells.

298 K8 that had been captured in anti-CK8-coated microtiter wells.

299 nd reduced GBS binding to fibronectin-coated microtiter wells.

300 ormat onto the bottom of streptavidin-coated microtiter wells.