ライフサイエンスコーパス: midbody

1 GTPase in the post-mitotic stabilisation of midbody.

2 neddylation and localization of Cul3 to the midbody.

3 nserved component of the spindle midzone and midbody.

4 at the centrosome, with none in the spindle midbody.

5 that it is the target of Cul3(KLHL21) at the midbody.

6 e plasma membrane at the cleavage furrow and midbody.

7 dle poles, centromeres, central spindle, and midbody.

8 SG101 from interacting and localizing to the midbody.

9 and organization of the spindle midzone and midbody.

10 , kinetochores, the mitotic spindle, and the midbody.

11 they remained tethered to each other at the midbody.

12 embrane, kinetochores, spindle poles and the midbody.

13 t much of the ESCRT pathway localizes to the midbody.

14 s localization is separable from that at the midbody.

15 and G(ialpha3) in the centrosomes and at the midbody.

16 terized by the concentration of KIF14 at the midbody.

17 resulting in entrapment of chromatin in the midbody.

18 IP3 and Rab11 localisation to the furrow and midbody.

19 of a pool of cIAP1 that associated with the midbody.

20 ocalizes to mitotic spindle poles and to the midbody.

21 le, and later is concentrated in the spindle midbody.

22 e events, as it localizes to the cytokinetic midbody.

23 , particularly the spindle, centrosomes, and midbody.

24 ng annexin 11 fail to establish a functional midbody.

25 also accumulates at the cleavage furrow and midbody.

26 an elongated, unsevered, and taxol-sensitive midbody.

27 cant amount of Plk3 was also detected in the midbody.

28 s exist to recruit ESCRT-III proteins to the midbody.

29 equency of aneuploidy and persistence of the midbody.

30 lear pore defects, and tension forces at the midbody.

31 able anchoring of the plasma membrane at the midbody.

32 ize to the plasma membrane or to the spindle midbody.

33 e accumulation of the exocyst complex at the midbody.

34 evented trafficking of Rab8a vesicles to the midbody.

35 number of cells where spartin is present at midbodies.

36 sis accompanied by an increase in unresolved midbodies.

37 n and that they localized to centrosomes and midbodies.

38 orm distinct bands associated with telophase midbodies.

39 ar envelopes, centrosomes, kinetochores, and midbodies.

40 sis in animal cells is the abscission of the midbody, a cytoplasmic bridge that connects the two pros

41 the attachment of the plasma membrane to the midbody, a long-postulated function of this organelle.

42 Plk1 displays little dynamic exchange at the midbody, a process that again is modulated by the kinase

43 es forming the central spindle organizes the midbody, a structure that anchors the ingressed cleavage

44 termed abscission, requires breakage of the midbody, a thin membranous stalk connecting the daughter

45 Time-lapse microscopy shows that only midbody abscission but not further upstream events, such

46 In contrast, quantitative analysis of midbody abscission showed inheritance of the midbody rem

47 m coming back together, and to contribute to midbody abscission.

48 the localization of OGT was decreased at the midbody after Aurora inhibition.

49 inetochore, centrosome, spindle midzone, and midbody, all of which are known to play critical roles i

50 s by blocking syntaxin-2 localization to the midbody, an event essential for completion of cytokinesi

51 otype consistent with destabilization of the midbody and abscission failure.

52 Spartin F24D does not localize to the midbody and acts in a dominant-negative manner to impair

53 for the targeting of apical endosomes to the midbody and apical membrane initiation site (AMIS) durin

54 ansferase (H3K4MT) complexes, resides at the midbody and associates with a subset of midbody regulato

55 egments and the O-P equivalence group of the midbody and caudal segments constitute distinct developm

56 In the midbody and caudal segments, each segmental repeat of ec

57 In the 28 midbody and caudal segments, however, there are two init

58 x and maintaining an association between the midbody and cell cortex.

59 ons regulate its movement from centrosome to midbody and completion of abscission.

60 machinery mediating AMIS recruitment to the midbody and determined that both branched actin and micr

61 nsport (ESCRT) proteins are recruited to the midbody and direct the severing of the intercellular bri

62 e that individual vesicles accumulate at the midbody and generate a reserve vesicle pool that is dist

63 Second, the midbody and midbody ring are released into a specific da

64 nce Plk1 is degraded can Cep55 target to the midbody and promote abscission.

65 This EB3 phosphorylation was enriched at the midbody and shown to control cortical microtubule growth

66 mportant for the recruitment of MITD1 to the midbody and that MITD1 participates in the abscission ph

67 is important for spartin recruitment to the midbody and that spartin participates in cytokinesis.

68 tion leads to elevated levels of Plk1 at the midbody and the failure of Cep55 recruitment.

69 severed the ventral nerve cord of leeches in midbody and then made video and in situ extracellular re

70 Arf6 is localised to the furrow and midbody and we show that Arf6-GTP functions to localise

71 either of these complexes is targeted to the midbody and whether their delivery is coordinated.

72 in centrosomes, mitotic spindles, midzones, midbodies, and cilia are all supportive of roles in micr

73 Smurf2 localizes to the centrosome, mitotic midbody, and centromeres.

74 Spartin colocalizes with Ist1 at the midbody, and depletion of Ist1 in cells by small interfe

75 e catalytic activity of Plk1 to centrosomes, midbody, and kinetochores.

76 alizes to the centrosomes, kinetochores, the midbody, and nuclear envelopes during the cell cycle.

77 at the tips of cortical microtubules and the midbody, and perturbation of GEF-H1 function induced mit

78 rosomes, interkinetochore/centromere region, midbody, and pseudocleavage furrows without DNA damage a

79 ocalize to centrosomes, mitotic spindles and midbody, and selective inhibition or silencing of TBK1 t

80 tokinesis, loss of Polo-like kinase-1 at the midbody, and the accumulation of binucleate cells.

81 tein ELKS and Rab8A-positive vesicles to the midbody, and the depletion of ELKS and Rab8A also leads

82 Electron-dense material and midbodies are both absent from the elongated midzones, a

83 Deriving from midzones, midbodies are organized by a set of microtubule interact

84 structural and regulatory components of the midbody are either absent or mislocalized.

85 ar region and subsequently trafficked to the midbody area during later stages of cytokinesis.

86 nt with Rab14's reported localization to the midbody as well as its effects upon Cdc42.

87 l for both the formation and function of the midbody, assembles in a sharp band at the centre of the

88 Despite its importance, understanding of midbody assembly and its regulation is still limited.

89 s represent the most detailed description of midbody assembly and maturation to date and may help elu

90 KIF20B is not required for midbody assembly, but may accelerate or coordinate midbo

91 of Plk1, a key regulator of cytokinesis, in midbody assembly.

92 an intervening spindle and chromosomes, the midbodies associated with ectopic furrows also contained

93 , an actin disassembly factor, in organizing midbody-associated protein complexes.

94 ends, as well as mislocalization of several midbody-associated proteins.

95 We propose that MICAL3 acts as a midbody-associated scaffold for vesicle targeting, which

96 It was additionally observed in the midbody at cytokinesis.

97 crotubule-rich intercellular bridge with the midbody at its centre.

98 e is concentrated at the mitotic spindle and midbody at M phase.

99 e at metaphase, and localizes to the spindle midbody at telophase.

100 romeres in metaphase and translocated to the midbody at telophase.

101 ingressing from basal to apical, forming the midbody at the apical membrane.

102 PRK2 is required for abscission of the midbody at the end of the cell division cycle and for ph

103 t of the cell cycle but then migrates to the midbody at the start of cytokinesis, suggesting that the

104 cell division and some Orc6 localizes to the midbody before cells separate.

105 At the midbody, BRCA2 influences the recruitment of endosomal s

106 spartin does not affect Ist1 localization to midbodies but markedly impairs cytokinesis.

107 b localizes predominately to centrosomes and midbodies, but also to spindle microtubules and transien

108 scribe how the structural composition of the midbody changes during progression throughout cytokinesi

109 Centralspindlin kinesin-6/RhoGAP complex, a midbody component critical for both the formation and fu

110 ion of the nonmuscle myosin II NMY-2 and the midbody component CYK-7 at the bridge, in part by limiti

111 nd cytoplasmic organelles but exclude normal midbody components such as MKLP1 and Aurora B.

112 Recruitment of WDR5 to the midbody dark zone appears to require integrity of the WD

113 Functional dissection of the midbody demonstrated the importance of lipid rafts and v

114 The midbody directs the final membrane abscission reaction a

115 eir maintenance or proliferation and then at midbodies during abscission, thereby helping ensure the

116 at spindle poles during mitosis and then at midbodies during cytokinesis, implying that these protei

117 and bidirectional growth of microtubules in midbodies during cytokinesis.

118 6-GTP functions to localise FIP3 and FIP4 to midbodies during cytokinesis.

119 metaphase and to the spindle midzone and the midbody during anaphase and cytokinesis.

120 4 and PRC1 colocalize on the spindle midzone/midbody during anaphase and cytokinesis.

121 It concentrates at the cleavage furrow and midbody during anaphase, telophase, and cytokinesis, imp

122 led-coil protein centriolin localized to the midbody during cytokinesis and was required for abscissi

123 CRT-associated protein-were recruited to the midbody during cytokinesis by interaction with centrosom

124 obrevin/VAMP-8, specifically localize to the midbody during cytokinesis in mammalian cells.

125 arase localizes to the ingressing furrow and midbody during cytokinesis in the C. elegans embryo.

126 ome in interphase cells and recruited to the midbody during cytokinesis, is a regulator required for

127 cell junctions and in mitotic cells, at the midbody during cytokinesis.

128 oles from interphase to telophase and at the midbody during cytokinesis.

129 phase and concentrate on the central spindle/midbody during cytokinesis.

130 lizes with MyoGEF at the central spindle and midbody during cytokinesis.

131 nteraction mediates MITD1 recruitment to the midbody during cytokinesis.

132 some targeting and AMIS formation around the midbody during epithelial lumenogenesis.

133 lizes to the cellular cortex and to the cell midbody during exit from mitosis and is consistent with

134 n interphase cells, which relocalizes to the midbody during late phases of mitosis.

135 o the spindle midzone in anaphase and to the midbody during normal cytokinesis.

136 OGT colocalized to the midbody during telophase with Aurora B.

137 alizes to microtubules in interphase and the midbody during telophase, and its protein levels decreas

138 ing anaphase, and to the cleavage furrow and midbody during telophase.

139 cate a novel mechanism for the regulation of midbody dynamics in which ARF6 protects the compacted ce

140 malian cells and is also enriched in spindle midbody extracts.

141 r (which induces a P-to-O fate change in the midbody) fails to affect O/P patterning in the rostral r

142 pindle structures, reduction of K-fibers and midbody fibers, disrupted chromosome movement, and atten

143 increases localization of active RHOA at the midbody, followed by diffusion to the flanking zones.

144 wo new tricellular tight junctions flank the midbody following cytokinesis.

145 the appropriate level of active RHOA at the midbody for faithful completion of cytokinesis and genom

146 , but it is essential for the proper midzone/midbody formation and cytokinesis in mammalian cells.

147 e furrow, and both proteins are required for midbody formation and proper localization of Pavarotti a

148 ndle during anaphase is essential for proper midbody formation and the segregation of daughter cells

149 diomyocytes after apical resection exhibited midbody formation consistent with abscission failure.

150 cytokinesis from embryonic hearts exhibited midbody formation consistent with successful abscission,

151 light chain and localized at the site of the midbody formation in dividing wild-type erythroblasts.

152 motility along microtubules in vitro and for midbody formation in vivo.

153 Finally, we show that CIT-K's role in midbody formation is conserved in human cells.

154 t the site of cleavage furrow ingression and midbody formation, and dominant negative ARF6 remains cy

155 hat both motor and stalk domains function in midbody formation, whereas the tail is essential for com

156 lays both structural and regulatory roles in midbody formation.

157 hat CIT-K is likely to act at the top of the midbody-formation hierarchy by connecting and regulating

158 3-3 for binding to centralspindlin such that midbodies formed by centralspindlin mutants that can bin

159 ein 14 (Tex14), which has been implicated in midbody function, is recruited to KTs by Plk1 in a Cdk1-

160 of both head and tail brains, implying that midbody ganglia contain a CPG for step production.

161 h receptors with axons in posterior roots of midbody ganglia.

162 each daughter, the smaller cluster near the midbody gradually migrates to rejoin the major cluster o

163 (which induces an O-to-P fate change in the midbody) has no effect in the rostral region.

164 g (which effects a P-to-O fate change in the midbody) has no effect on the patterning of O and P fate

165 Although several essential components of the midbody have recently been identified, essential compone

166 )1 subfamily present in central spindles and midbodies in mammalian cells.

167 26 or Ser-28 is localized to centrosomes and midbodies in the absence of DNA damage.

168 ed on chromatin bridges and near the midzone/midbody in an AIR-2-dependent manner.

169 lin ring complex of early mitosis and to the midbody in late cytokinesis by immunofluorescence assay

170 1 to centrosomes in early mitosis and to the midbody in late mitosis.

171 Annexin 11 is recruited to the midbody in late telophase, where it forms part of the de

172 may be related to the specialization of the midbody in neural cells.

173 d localization of both KIF14 and CRIK at the midbody in patient-derived fibroblasts.

174 n abscission, as it fails to localize to the midbody in SCCRO-deficient cells during abscission, and

175 metaphase, while it was concentrated at the midbody in telophase in meiotic oocytes.

176 st that WDR5 is specifically targeted to the midbody in the absence of chromatin and that it promotes

177 red somatic cells, TEX14 can localize to the midbody in the absence of other germ cell-specific facto

178 The midbody is a transient structure that connects two daugh

179 t, we find that the targeting of FIP3 to the midbody is independent of Rab11.

180 We show that after cytokinesis, the midbody is inherited by one of the daughter cells as a r

181 ork has shown that targeted secretion to the midbody is required to complete cell division.

182 elch protein KLHDC8B, which localizes to the midbody, is expressed during mitosis, and is mutated in

183 s (rostrum body, anterior midbody, posterior midbody, isthmus, and splenium) and for overall CC size,

184 ns had greater connectivity in the posterior midbody/isthmus of the corpus callosum and that fraction

185 ensable for the formation of the midzone and midbody, it promotes contractility and is required for t

186 elective, as other nonmembranous organelles (midbody, kinetochore) and membrane-bounded organelles (m

187 g the NH(2)-terminal domain required for the midbody localization but lacking the COOH-terminal catal

188 MLL1 binding to this pocket also abolish the midbody localization of WDR5.

189 il play distinctive roles in stabilizing the midbody matrix and maintaining an association between th

190 exogenous CHO1 restored the formation of the midbody matrix and rescued cytokinesis in siRNA-treated

191 idzone during anaphase and concentrates to a midbody matrix during cytokinesis.

192 tial for completion of cytokinesis after the midbody matrix has formed.

193 interference also affected the formation of midbody matrix in dividing cells, caused the disorganiza

194 nucleate cells because of the absence of the midbody matrix in the middle of the intercellular bridge

195 ating protein (MgcRacGAP) and converts these midbody matrix proteins into stable intercellular bridge

196 ules during anaphase, and assembles into the midbody matrix surrounding the compacted midzone microtu

197 ning cytoplasmic bridge from which the dense midbody matrix was missing.

198 the midzone and prevents the formation of a midbody matrix.

199 y assembly, but may accelerate or coordinate midbody maturation.

200 The midbody mediates abscission by recruiting many factors,

201 cally regulate the localization of lipids to midbodies, membrane-based structures where cleavage occu

202 , apparently because the spindle midzone and midbody microtublues were absent during late mitosis.

203 ingression and abscission, during which the midbody microtubule bundle provides both structural supp

204 promotes abscission, perhaps by facilitating midbody microtubule disassembly.

205 Additionally, EB3 promoted midbody microtubule stability and, consequently, midbody

206 y regressed, even though spindle midzone and midbody microtubules appeared normal.

207 ily member, and cIAP1 were both localized on midbody microtubules at telophase, and also interacted w

208 ies of KIF14 are well suited for stabilizing midbody microtubules during cytokinesis.

209 Consistent with these defects, midbody microtubules in WDR5 knockdown cells also displa

210 ociation between the plasma membrane and the midbody microtubules prior to abscission.

211 ing-derived midbody ring, it is assumed that midbody microtubules scaffold the abscission machinery.

212 In this paper, we assess the contribution of midbody microtubules versus the midbody ring in the Caen

213 Surprisingly, midbody microtubules were dispensable for both stages.

214 entral role of the midbody ring, rather than midbody microtubules, in their execution.

215 illomavirus E2 colocalizes with MKlp2 in the midbody/midplate during late mitosis.

216 SSRP1 colocalizes with the spindle and midbody MTs, and associates with MTs both in vitro and i

217 s produced by the rostral OP pathway and the midbody O and P pathways are very similar.

218 at centrosomes and are then recruited to the midbodies of dividing cells through direct interactions

219 IST1, CHMP1, and VPS4 were recruited to the midbodies of dividing cells, and depleting either IST1 o

220 entrosomal compartment and especially in the midbody of dividing cells.

221 vage furrow membranes and accumulated at the midbody of dividing embryos in a manner similar to dynam

222 in that localizes to the cleavage furrow and midbody of mitotic cells, where it is required for the c

223 c I-2 localized to the spindle, midzone, and midbody of mitotic human epithelial ARPE-19 cells.

224 ired for cytokinesis and is localized on the midbody of the contractile ring.

225 l Plk is localized at the centrosome and the midbody of transfected cells as shown previously for ful

226 from the inside out by the microtubule-based midbody or from the outside in by the contractile ring-d

227 Thus, Kif20b is required for proper midbody organization and abscission in polarized cortica

228 0b is involved in cerebral cortex growth and midbody organization of neural stem cells.

229 ter capillary area in the pes (p = .002) and midbody (p = .021).

230 n groups in whole hippocampus (p = .013) and midbody (p = .036).

231 in the anterior (pes, p = .042) and central (midbody, p = .004) DG, and greater capillary area in the

232 and mark the center of the cell in lieu of a midbody/phragmoplast.

233 cal domain, mitotic spindle orientation, and midbody position, consistent with Rab14's reported local

234 r several CC regions (rostrum body, anterior midbody, posterior midbody, isthmus, and splenium) and f

235 trosome protein 55 (Cep55), a centrosome and midbody protein essential for abscission.

236 ic and microtubule disassembly at a specific midbody region called the secondary ingression.

237 to dorsal and then centripetally toward the midbody region on the dorsal basement membrane.

238 DTCs continue their migration away from the midbody region.

239 lineages in the segments of the rostral and midbody regions.

240 the midbody and associates with a subset of midbody regulatory proteins, including PRC1 and CYK4/MKL

241 division factors to the spindle midzone and midbody remain unclear.

242 midbody abscission showed inheritance of the midbody remnant by one daughter cell, indicating that cy

243 ing and, in late telophase, KRIT1 stains the midbody remnant most strongly; this is the site of cytok

244 Tethered midbody remnants dancing across apical microvilli, encou

245 indle, and in most cases, dissolution of the midbody, reopening of the cleavage furrow, and realignme

246 In contrast, the contractile ring and midbody required for cytokinesis, the final stage of mit

247 otect against genetic damage by coordinating midbody resolution with the abscission checkpoint.

248 cytokinesis resolves with abscission of the midbody, resulting in independent daughter cells, germ c

249 in that only one daughter cell inherits the midbody ring (MR) upon completion of abscission even in

250 e ring (CR) is coupled to the formation of a midbody ring (MR), through poorly understood mechanisms.

251 Second, the midbody and midbody ring are released into a specific daughter cell

252 The vesicles move to the midbody ring asymmetrically from one prospective daughte

253 n through this stage required the septins (a midbody ring component) but not the membrane-remodeling

254 tribution of midbody microtubules versus the midbody ring in the Caenorhabditis elegans embryo.

255 embrane scission occurs on both sides of the midbody ring with random order and that completion of th

256 e outside in by the contractile ring-derived midbody ring, it is assumed that midbody microtubules sc

257 ission and highlight the central role of the midbody ring, rather than midbody microtubules, in their

258 lation of v-SNARE-containing vesicles at the midbody ring.

259 n, and septins and in forming the subsequent midbody ring.

260 uire centriolin for localization to a unique midbody-ring structure, and disruption of either complex

261 tors associated with longitudinal muscles in midbody segments of medicinal leeches, only the ventral

262 tion differ substantially in the rostral and midbody segments, even though the set of differentiated

263 However, apical midbodies show changes in number, shape and positioning

264 ody microtubule stability and, consequently, midbody stabilization necessary for efficient cytokinesi

265 ith addition of an Aurora B inhibitor at the midbody stage suggests that Aurora B is the target of SC

266 ng multinucleation and cells arrested at the midbody stage, which are rescued by ectopic expression o

267 is also sufficient to increase the number of midbody-stage cells and, likewise, triggers distinctive

268 We further find that ATR activity in midbody-stage cells links postmitotic genome surveillanc

269 the pore basket components Tpr and Nup 50 in midbody-stage cells.

270 ge of cytokinesis, Cep55 is required for the midbody structure and for the completion of cytokinesis.

271 specific role for BRCA2 in the regulation of midbody structure and function, separate from DNA damage

272 ndlin to Cep55, which, in turn, controls the midbody structure and membrane fusion at the terminal st

273 ed during mitosis, where it localizes to the midbody structure connecting cells about to separate dur

274 It is currently unclear how the midbody structure is stably maintained during this perio

275 OS7 cells, LGN moves from the nucleus to the midbody structure separating daughter cells during the l

276 olymerization at the edge of the cytokinetic midbody structure, located at the center of the intercel

277 of cardiomyocytes accompanied by unresolved midbody structures.

278 h includes peristalsis-like movements of the midbody that are organized into both rostral-to-caudal a

279 positive vesicles at the cleavage furrow and midbody that is not a consequence of chromosome nondisju

280 ere we show that BRCA2 is a component of the midbody that is recruited through an interaction with Fi

281 and the ESCRT-associated protein ALIX to the midbody (the structure that tethers two daughter cells)

282 nts, the various observed transitions of the midbody, the beginning of ciliogenesis, and the accumula

283 s the three microtubule-based organelles-the midbody, the centrosome, and the cilium-in the same cell

284 ps that relocalize to different parts of the midbody: the bulge, the dark zone, and the flanking zone

285 is recruited to the central spindle and the midbody through a direct interaction with the centralspi

286 thway, ALIX and TSG101, are recruited to the midbody through direct interactions with the phosphoprot

287 s to microtubules of the central spindle and midbody throughout cytokinesis, at sites distinct from t

288 ed rupture of the hypodermis, failure of the midbody to elongate properly, abnormal contacts between

289 microtubule motor protein, localizes at the midbody to finalize cytokinesis by interacting with CRIK

290 along the intercellular bridge away from the midbody until it reaches an equilibrium position, determ

291 ination-promoted turnover of Aurora B at the midbody was deficient in SCCRO- and KLHL21-deficient cel

292 ures, such as centrosomes, kinetochores, and midbody, we propose that the observed feed-forward mecha

293 Midbodies were isolated from mammalian cells, proteins w

294 the centrosomes, the spindle poles, and the midbody when ectopically expressed in HeLa and U2OS cell

295 m recycling endosomes to the cleavage furrow/midbody where they are tethered prior to fusion events v

296 ed to presumptive abscission sites in mature midbodies, where they may regulate the endosomal sorting

297 During cytokinesis, LARG is condensed in the midbody, where it colocalizes with RhoA.

298 nimal cells requires the central spindle and midbody, which contain prominent microtubule bundles.

299 atic cells concludes with the formation of a midbody, which is abscised to form individual daughter c

300 of both the spindle within the cell and the midbody within the mitotic spindle, with differential re