ライフサイエンスコーパス: middle promoter

1 A contacts within the -20 to -40 region of a middle promoter.

2 egion 4 and with a DNA element present in T4 middle promoters.

3 cherichia coli transcription apparatus to T4 middle promoters.

4 nto middle genes and by the activation of T4 middle promoters.

5 the T4 MotA protein, for activation of phage middle promoters.

6 sor and Ndt80 activator for occupancy at key middle promoters.

7 polymerase are needed to transcribe from T4 middle promoters.

8 rs and coactivating transcription from phage middle promoters.

9 ential co-activator of transcription from T4 middle promoters.

10 MotA alone binds to the -30 region of T4 middle promoters, a region that contains the MotA box co

11 how that AsiA also plays a direct role in T4 middle promoter activation by contacting the MotA activa

12 According to the current model for T4 middle promoter activation, AsiA plays an indirect role

13 findings, we propose a revised model for T4 middle promoter activation, with AsiA organizing the act

14 Middle-promoter activation requires the T4 transcription

15 nce 5'TTTGCTTTA3' (positions -34 to -26 of a middle promoter) affects MotA function.

16 onsistent with data on how MotA functions at middle promoters, and provides an explanation for why Mo

17 Middle promoters are controlled through DNA elements ter

18 n, the MotA transcription factor binds to T4 middle promoters at a -30 mot box consensus sequence to

19 pressor and Ndt80 activator proteins control middle promoters by binding to overlapping DNA elements.

20 Middle promoters contain a -10 region recognized by the

21 T4 middle promoters contain the sigma70 -10 DNA element.

22 k interaction of MotA with the -30 region of middle promoter DNA.

23 An inducible middle promoter from the lactococcal bacteriophage phi31

24 The middle promoter has a -10 hexamer, but no -35 hexamer.

25 Bacteriophage T4 middle promoters have excellent matches to the -10 conse

26 that the Sum1 repressor can be removed from middle promoters in meiotic cells independent of Ndt80 e

27 , KVP40 AsiA activates transcription from T4 middle promoters in the presence of T4 MotA.

28 ole as co-activator, AsiA directs RNAP to T4 middle promoters in the presence of the T4-encoded activ

29 n in vitro, and we have identified seven new middle promoters in vivo.

30 iring transcription from MotA/AsiA-activated middle promoters in vivo.

31 It has been proposed that the induction of middle promoters is controlled by competition between Nd

32 ription by unmodified polymerase from the T4 middle promoter P uvsx is independent of the specific se

33 teraction of the alpha CTD with the phage Mu middle promoter P(m) and its activator protein Mor.

34 s, Mot21 and MotA bind DNA containing the T4 middle promoter P(uvsX) similarly, and the proteins yiel

35 required for MotA/AsiA activation at the T4 middle promoter P(uvsX).

36 Transcription from the middle promoter, P(m), requires the phage-encoded activa

37 Consistent with these requirements, the middle promoter, Pm, has a -10 hexamer but lacks a recog

38 Consistent with these requirements, the middle promoter, Pm, has a recognizable -10 region but l

39 Transcription from the middle promoter, Pm, of bacteriophage Mu requires the ph

40 Transcription from the middle promoter, Pm, of phage Mu is initiated by Escheri

41 subjected to a comparative analysis: the T4 middle promoter PrIIB2 opens and closes thermoreversibly

42 r the binding of polymerase/MotA/AsiA to the middle promoter PuvsXis inhibited by premethylation of g

43 anscriptional activation of bacteriophage T4 middle promoters requires sigma70-containing Escherichia

44 and the phage genome appears to lack typical middle promoter sequences, KVP40 AsiA activates transcri

45 The identification of more than 20 middle promoters suggested that a strong match to the Mo

46 r activate transcription in vitro using a T4 middle promoter that is active with RNA polymerase alone

47 romoters and promotes transcription at phage middle promoters through its interaction with the primar

48 Transcription from bacteriophage T4 middle promoters uses Escherichia coli RNA polymerase to

49 s involved in N4 RNAPII transcription and of middle promoters, we propose a model for N4 RNAPII promo

50 s as a co-activator of transcription from T4 middle promoters, which retain the canonical sigma70 -10

51 Coli RNA polymerase transcribes from middle promoters with or without the activator.