ライフサイエンスコーパス: midzone

1 tiparallel microtubule overlap at the cell's midzone.

2 se of restoring MT engagement in the spindle midzone.

3 erlies the explosive assembly of the spindle midzone.

4 spindlin to accumulate stably at the spindle midzone.

5 from the chromosomes to the anaphase spindle midzone.

6 of multiple furrows that ingress toward the midzone.

7 uction of microtubule density in the spindle midzone.

8 y in anaphase that is centred at the spindle midzone.

9 ke the form of articular chondrocytes in the midzone.

10 ithin the cleavage furrow around the spindle midzone.

11 hed the localization of Cep55 to the spindle midzone.

12 tely a reduction of ZEN-4-GFP at the spindle midzone.

13 somes, spindle microtubules, and the spindle midzone.

14 t imparts matrix-like characteristics to the midzone.

15 to the mitotic kinetochores and the spindle midzone.

16 h targets and maintains ZEN-4 at the spindle midzone.

17 localized to chromosomes and to the spindle midzone.

18 allel near the poles and antiparallel in the midzone.

19 liding antiparallel MTs apart in the spindle midzone.

20 that include the kinetochore and the spindle midzone.

21 in metaphase and then with anaphase spindle midzone.

22 or pushing hal-spindles apart in the spindle midzone.

23 opposing the force generated by the spindle midzone.

24 ndle by promoting instability of the spindle midzone.

25 e, it is localized indirectly to the spindle midzone.

26 nd promoting transport of the complex to the midzone.

27 ho-interacting kinase citron to the anaphase midzone.

28 stricting centromere movement to the spindle midzone.

29 ity with higher turnover rates nearer to the midzone.

30 e enrichment of Cin8 and Kip1 at the spindle midzone.

31 ty of the microtubules polymerize toward the midzone.

32 s were located mainly in the superficial and midzones.

33 actors, Aurora B and Kif4, act as they do in midzones.

34 m for investigating plus-end organization in midzones.

35 the centromeres and relocates to the spindle midzone [7].

36 The spindle midzone, a conspicuous network of antiparallel interdigi

37 ision depends on the assembly of the spindle midzone, a specialized array of overlapping microtubules

38 e ase1p-mediated recruitment of klp9p to the midzone accelerates pole separation, as suggested by com

39 the anti-parallel microtubule overlap of the midzone, after which the MKlp2-CPC complex moves in a no

40 Midzones, also called central spindles, are an array of

41 e equator by centralspindlin, Ect2's spindle midzone anchor complex, and is temporally coordinated wi

42 ubule-bundling protein that localizes to the midzone and also to microtubule bundles in the cytoplasm

43 lize to the equatorial region of the spindle midzone and are capable of bundling antiparallel microtu

44 DdINCENP null cells lack a robust spindle midzone and are hypersensitive to microtubule-depolymeri

45 onset, when it redistributed to the spindle midzone and became concentrated at the equator along mid

46 both a very slow rate of turnover within the midzone and limited lateral diffusion along spindle MTs.

47 cytokinesis, apparently because the spindle midzone and midbody microtublues were absent during late

48 ow completely regressed, even though spindle midzone and midbody microtubules appeared normal.

49 ment of cell division factors to the spindle midzone and midbody remain unclear.

50 tion is dispensable for the formation of the midzone and midbody, it promotes contractility and is re

51 iorientation and organization of the spindle midzone and midbody.

52 omplex, a conserved component of the spindle midzone and midbody.

53 In general, MAPs and motors stabilize the midzone and motors produce sliding.

54 e that CPCs function to organize the spindle midzone and potentially switch motors between force gene

55 lization of MKLP1, PRC1, and Aurora B to the midzone and prevents the formation of a midbody matrix.

56 e intercellular canal containing the spindle midzone and resealing of the daughter cells.

57 actin cables in a gap between the monopolar midzone and the furrow-like cortex, suggesting a communi

58 rometaphase and metaphase and to the spindle midzone and the midbody during anaphase and cytokinesis.

59 ntrosome during metaphase and to the spindle midzone and the midbody during anaphase and telophase.

60 was also concentrated on MTs in the spindle midzone and the phragmoplast.

61 strate that dynamin localizes to the spindle midzone and the subsequent intercellular bridge in mamma

62 entional dynamin is recruited to the spindle midzone and the subsequent intercellular bridge, where i

63 o centrosomes, kinetochores, and the spindle midzone and what the specific tasks of Plk1 at these dis

64 t a specific time during cytokinesis, making midzones and mitotic spindles differ in their dynamics a

65 bidopsis, ATK5, localizes to mitotic spindle midzones and regions rich in growing plus-ends within ph

66 bundle microtubules), construct the spindle midzone, and complete cytokinesis.

67 us and ectopic I-2 localized to the spindle, midzone, and midbody of mitotic human epithelial ARPE-19

68 uted to the kinetochore, centrosome, spindle midzone, and midbody, all of which are known to play cri

69 when SPD-1 is depleted, there is no visible midzone, and only this furrow localization remains.

70 ein associated with centromeres, the spindle midzone, and poles during mitosis and the cleavage furro

71 ested CR components, is anchored at the cell midzone, and this physical property is likely to account

72 midbodies are both absent from the elongated midzones, and actin filaments from the furrow cortex are

73 Caenorhabiditis elegans disrupt the spindle midzone (anti-parallel microtubules and associated prote

74 dle are selected and organized into a stable midzone array are poorly understood.

75 rincipal role in organizing the antiparallel midzone array, we sought to clarify their roles in monop

76 mbly as they do in spindles, whereas two key midzone assembly factors, Aurora B and Kif4, act as they

77 Therefore, in contrast to the linear midzone assembly pathway, centralspindlin and the CPC ma

78 ates mitotic exit but also modulates spindle midzone assembly through Sli15-Ipl1.

79 microtubule-associated proteins required for midzone assembly, can tag microtubule plus ends.

80 D-1 may play an early role in the pathway of midzone assembly.

81 A midzone-associated Aurora B gradient was found to monito

82 or protein that translocates PRC1, a spindle midzone-associated cyclin-dependent kinase substrate pro

83 In addition, we found that the Ase1 spindle midzone-associated protein is required for bipolar spind

84 We have examined the role of PRC1, a midzone-associated, microtubule bundling, Cdk substrate

85 is required for assembly of a robust spindle midzone at anaphase and for normal timings of spindle el

86 icrotubule antiparallel bundler ase1p at the midzone at anaphase B onset.

87 tes with the spindle and concentrates in the midzone at anaphase B.

88 zed onto the spindle pole bodies and spindle midzone at anaphase onset, where it persists into midana

89 the dispersion of aurora B from the spindle midzone at late anaphase; however, centromeric associati

90 10 to the spindle during anaphase and to the midzone at telophase.

91 osomal protein that transfers to the spindle midzone at the metaphase/anaphase transition.

92 though intact cortices, kinetochore MTs, and midzone augmentation are dispensable, this patronin-base

93 sly delaying aster separation and disrupting midzone-based signaling leads to complete failure of fur

94 anner and 2) it subsequently refines spindle midzone-based signaling to restrict furrowing to a singl

95 Disrupting midzone-based signaling, by depleting conserved midzone

96 e nonkinetochore microtubules in the spindle midzone become compacted into the central spindle, a str

97 omatids failed to separate completely at the midzone, becoming stretched to up to twice their normal

98 Despite this important role, many aspects of midzone biology remain unknown, including the dynamic or

99 does not localize to the cortex and spindle midzone but accumulates on spindle pole microtubules to

100 dle in the presence of Feo, including at the midzone, but the antibody-induced dissociation of Feo in

101 nsverse organization initiated at the cell's midzone by 45 min after induction and progressed bidirec

102 itates the targeting of ZEN-4 to the spindle midzone by mediating the posttranscriptional regulation

103 Midzones can be considered to be platforms that recruit

104 As a result, sliding forces within the midzone cannot buckle spindle microtubules, which allows

105 rated that WISP3 protein is expressed in the midzone chondrocytes of normal adult articular cartilage

106 when trailing chromatids are present at the midzone.Chromatid segregation must be coordinated with c

107 tein PRC1 plays a key role in organizing the midzone complex.

108 zone-based signaling, by depleting conserved midzone complexes, results in a converse phenotype: neit

109 We found that two other midzone components also localize to the ingressing furro

110 o, it can localize to the midzone when other midzone components are depleted, suggesting that SPD-1 m

111 e spindle midzone fails to form, even though midzone components are present.

112 SPD-1 differs from other midzone components in that it is essential for the integ

113 -1 is perturbed in embryos depleted of other midzone components, yet the cytoplasmic bundles are not

114 The spindle midzone-composed of antiparallel microtubules, microtubu

115 crotubules that self-organize at the spindle midzone contributes to positioning the cell-division pla

116 w Ect2, which is associated with the spindle midzone, controls RhoA activity at the equatorial cortex

117 naling by the centrosomal asters and spindle midzone coordinately directs formation of the cytokineti

118 pression of DdCP224 rescued the weak spindle midzone defect of DdINCENP null cells.

119 e show that loss of function of spd-1 causes midzone disruptions, although cytokinesis generally comp

120 e spindle midzone, thereby promoting spindle midzone dissolution.

121 It associates with the spindle midzone during anaphase and concentrates to a midbody ma

122 that ZEN-4 protein localizes to the spindle midzone during anaphase and persists at the midbody regi

123 anaphase and is concentrated at the spindle midzone during anaphase and telophase.

124 parts of mitosis, as well as to the spindle midzone during anaphase and the mid-body during telophas

125 plus-ends of interpolar microtubules at the midzone during anaphase, a process that requires survivi

126 ved along interpolar microtubules and at the midzone during anaphase.

127 e mutually dependent for localization to the midzone during cytokinesis.

128 ulate along microtubules towards the spindle midzone during late anaphase.

129 Staining was concentrated near the midzone during metaphase and was retained there during a

130 localized to the chromosomes and then to the midzone during mitosis, but the mutated forms are detect

131 s, which functions in organizing the spindle midzone during mitosis.

132 amics to gamma-tubulin, moving from poles to midzone during the anaphase-to-telophase transition.

133 In the absence of KIF4, midzones elongate abnormally, and their overlap regions

134 h regulation appears to occur by terminating midzone elongation at a specific time during cytokinesis

135 KIF4 is required to terminate midzone elongation in late anaphase.

136 ess but subsequently regress and the spindle midzone fails to form, even though midzone components ar

137 Under these conditions, the spindle midzone fails to organize and function properly.

138 anslocation of PRC1 by Kif4 is essential for midzone formation and cytokinesis.

139 logues of CHO1 were shown to function in the midzone formation and cytokinesis.

140 PRC1 translocation and midzone formation can be restored, and the cytokinetic d

141 Midzone formation is also inhibited in fly spermatocytes

142 bition of PRC1 translocation, a block of the midzone formation, and a failure of cytokinesis.

143 had defects in chromosome behavior, spindle midzone formation, and cytokinesis.

144 lts, in light of the crucial role of PRC1 in midzone formation, indicate that cell cycle-dependent tr

145 ralspindlin in a linear pathway that governs midzone formation.

146 hromosome-microtubule attachment and spindle midzone formation.

147 Spindle midzone fragments not connected to either of the two spi

148 The spindle midzone harbors both microtubules and proteins necessary

149 brane domain surrounding the mitotic spindle midzone, here named the midzone membrane domain (MMD), i

150 strates colocalize with Ipl1p to the spindle midzone, identifying additional proteins that may regula

151 indle translocate continuously away from the midzone in a phenomenon called poleward microtubule flux

152 t not condensin II, localizes to the spindle midzone in anaphase and to the midbody during normal cyt

153 s at the spindle pole bodies and the spindle midzone in anaphase cells.

154 t block Aurora B localization to the spindle midzone in anaphase or Aurora B function in cytokinesis.

155 pindle poles in metaphase and to the spindle midzone in anaphase.

156 gulating the spatiotemporal formation of the midzone in HeLa cells.

157 trolling the spatiotemporal formation of the midzone in human cells.

158 protein, Ndc10p, is enriched at the spindle midzone in late anaphase.

159 inhibiting Plk1 caused premature assembly of midzones in cells still in metaphase, breaking the tempo

160 erial and resemble similar foci in monopolar midzones in cells.

161 orylated at centromeres and anaphase spindle midzones in vivo.

162 ink antiparallel microtubules at the spindle midzone including BIMC, MKLP, and CENP-E are closely rel

163 ring proteins on the equatorial cortex in a midzone-independent manner and 2) it subsequently refine

164 at an outward force generated by the spindle midzone, independently of centrosomes, is sufficient to

165 ued the localization of ZEN-4 at the spindle midzone, indicating that ATX-2 mediates the localization

166 localizes to the mitotic spindle and spindle midzone, indicating that SUMO paralogs regulate distinct

167 The microtubule midzone is able to stimulate the cortex of the cell to e

168 her hand, the localization of the CPC in the midzone is independent of the borealin-HP1 interaction,

169 se1p at microtubule plus ends at the spindle midzone is more stable.

170 These studies reveal that the spindle midzone is necessary and sufficient for the stabilizatio

171 bundle of microtubules known as the spindle midzone is rapidly assembled after anaphase onset, recru

172 ted secretion in the vicinity of the spindle midzone is required for the execution of the terminal ph

173 Recent observations suggest that the spindle midzone is required for this process.

174 The midzone is the domain of the mitotic spindle that mainta

175 ure that cleavage occurs through the spindle midzone is unknown.

176 er metaphase, and accumulates at the spindle midzone late in anaphase.

177 ne plus-end dynamics whose activity controls midzone length but not stability.

178 KIF4-mediated midzone length regulation appears to occur by terminatin

179 spindle microtubule polymerization, limiting midzone length.

180 crotubule (MT) motor proteins at the spindle midzone, less is known about how microtubule-associated

181 the short Ase1 isoforms impairs the spindle midzone localization of full-length Ase1, it is likely t

182 The midzone localization of SPD-1 is perturbed in embryos de

183 Feo homologues in other systems enhance the midzone localization of the MT-MT cross-linking motors k

184 oss-linking motors kinesin-4, -5 and -6, the midzone localization of these motors is respectively enh

185 MT-dependent networks of spindle midzone MAPs may be one molecular basis for the postulat

186 the mitotic spindle midzone, here named the midzone membrane domain (MMD), is essential for spindle

187 l metaphase but subsequently transfer to the midzone microtubule array and the equatorial cortex duri

188 activity correlated with the distribution of midzone microtubule bundles and Telophase Disc 60 protei

189 onstrate that continuous interaction between midzone microtubule bundles and the cortex is required f

190 In addition, midzone microtubule bundles became destabilized and auro

191 iRNA abolishes this actin-myosin-independent midzone microtubule compaction.

192 rwent chromosome poleward movement, formed a midzone microtubule complex, and completed cytokinesis.

193 es both the exaggerated anaphase and reduced midzone microtubule density observed in early neural epi

194 Investigating midzone microtubule dynamics has been difficult in part

195 y may prevent cytokinesis through inhibiting midzone microtubule formation, the behavior of proteins

196 addition, HSET knockdown resulted in severe midzone microtubule organization, most notably at microt

197 Midzone microtubule stabilization also depends on the ki

198 integral for coupling furrow ingression with midzone microtubule stabilization.

199 lically associates with astral, spindle, and midzone microtubules and also to actomyosin pseudocleava

200 propose that ZEN-4 directly cross-links the midzone microtubules and suggest that these microtubules

201 issociates from centromeres and localizes to midzone microtubules and the equatorial cortex.

202 CaM kinase II appears localized on midzone microtubules as soon as they form and may have a

203 We suggest that cultured cells use midzone microtubules as the primary signaling pathway fo

204 crotubules at metaphase, then relocalizes to midzone microtubules at anaphase and regulates central s

205 ENP was detected in association with spindle midzone microtubules beneath sites of furrowing and was

206 naphase, hRif1 aligned along a subset of the midzone microtubules between the separating chromosomes.

207 ith centromeres during prometaphase and with midzone microtubules during anaphase and is required for

208 es throughout mitosis, organizes the spindle midzone microtubules during anaphase, and assembles into

209 the midbody matrix surrounding the compacted midzone microtubules during cytokinesis.

210 dback between aurora B kinase activation and midzone microtubules generates a gradient of post-transl

211 n metaphase and anaphase and associates with midzone microtubules in anaphase and telophase.

212 ssibility that microtubule polymerization of midzone microtubules is continuously required to sustain

213 mEg5 punctae on midzone microtubules moved transiently both toward and a

214 In addition, reduction of midzone microtubules near the cortex, by either nocodazo

215 egulated by both astral microtubules and the midzone microtubules of the mitotic apparatus.

216 evealed that mEg5 punctae on both astral and midzone microtubules rapidly bind and unbind.

217 However, motion of Eg5 on midzone microtubules was not altered.

218 ividing cells, caused the disorganization of midzone microtubules, and resulted in abortive cytokines

219 segregation of chromosomes, the bundling of midzone microtubules, and the initiation of cytokinesis

220 subsequent appearance of fluorescence along midzone microtubules, but not that near the lateral equa

221 former delivered centromeric aurora B along midzone microtubules, whereas the latter delivered cytop

222 the chromosomes dictates the organization of midzone microtubules, which in turn determines and maint

223 depend on the mitotic spindle apparatus and midzone microtubules.

224 r telophase II, becoming associated with the midzone microtubules.

225 localization of the ZEN-4 kinesin protein to midzone microtubules.

226 pindle poles and later in development on the midzone microtubules.

227 and became concentrated at the equator along midzone microtubules.

228 with centromeres or initial relocation onto midzone microtubules.

229 ctivated egg including the region containing midzone microtubules.

230 efect is preceded by the dissociation of the midzone microtubules.

231 pindlin complex that concentrates on spindle midzone microtubules.

232 e equator by the centralspindlin complex and midzone microtubules.

233 known, including the dynamic organization of midzone microtubules.

234 Deriving from midzones, midbodies are organized by a set of microtubul

235 ce of Arl3 in centrosomes, mitotic spindles, midzones, midbodies, and cilia are all supportive of rol

236 and Kif4 and PRC1 colocalize on the spindle midzone/midbody during anaphase and cytokinesis.

237 okinesis, but it is essential for the proper midzone/midbody formation and cytokinesis in mammalian c

238 s enriched on chromatin bridges and near the midzone/midbody in an AIR-2-dependent manner.

239 to an anti-parallel array termed the spindle midzone (midzone MTs), whereas F-actin and non-muscle my

240 on that is important for cytokinesis and for midzone MT stabilization following furrow ingression.

241 We show here that midzone MT stabilization is dependent on actomyosin cont

242 ous studies in somatic cells have shown that midzone MTs become highly stable after furrows have begu

243 ach marks showed that sliding of overlapping midzone MTs was responsible for the elongation of the sp

244 Stabilization of midzone MTs with low amounts of Taxol rescues cytokinesi

245 i-parallel array termed the spindle midzone (midzone MTs), whereas F-actin and non-muscle myosin II,

246 ads to the rapid depolymerization of spindle midzone MTs.

247 n NoCut, Aurora kinase (Ipl1) at the spindle midzone negatively regulates cytokinesis through two pro

248 in TOGp/XMAP215, was absent from the spindle midzone of DdINCENP null cells.

249 hibited greatly enhanced localization in the midzone of developing phragmoplast.

250 pression of the 3 proteins extended into the midzone of OA cartilage.

251 idopsis and is specifically localized to the midzone of the mitotic apparatus and phragmoplast.

252 s in mitotic cells where it localizes to the midzone of the spindle during anaphase, and to the cleav

253 s, but later accumulates specifically at the midzone of these same spindles during telophase.

254 , phosphorylated myosin first appears in the midzones of the separating chromosomes during late anaph

255 t form in the absence of associated spindle, midzone, or chromosomes.

256 Disruption of AIR-2, the spindle midzone, or condensin leads to cytokinesis failure in a

257 ule-associated proteins (MAPs) contribute to midzone organization and function.

258 chromosomal passenger complex (CPC) promotes midzone organization, specifies the cleavage plane, and

259 that affect chromosome behavior and spindle midzone organization.

260 mployed monopolar cytokinesis to reveal that midzone plus ends appear to be nondynamic.

261 hromokinesin KIF4 as a negative regulator of midzone plus-end dynamics whose activity controls midzon

262 report in Science a mechanism of asymmetric midzone positioning driven by a polarized cortical distr

263 s critical to ensure that furrow and spindle midzone positions coincide throughout cytokinesis.

264 ess, we found that Ase1, a conserved spindle midzone protein [5], appeared as two short protein isofo

265 We show that the spindle midzone proteins kinesin-5 cut7p and MT bundler ase1p co

266 them, focusing on the conserved cytokinesis midzone proteins Prc1 and Kif4A.

267 neighboring asters and recruited cytokinesis midzone proteins, including the chromosomal passenger co

268 f cytokinesis and speculate that the spindle midzone region of animal cells is functionally equivalen

269 rpolar MT bundles, being concentrated in the midzone region.

270 anaphase, binding to RacGAP1 at the spindle midzone repositions Ect2 to induce local actomyosin ring

271 cumulation of centralspindlin at the spindle midzone requires its multimerization into clusters and A

272 nsity analysis of this bundle at the spindle midzone showed a preferred spacing of approximately 35 n

273 t Ase1p is a member of a conserved family of midzone-specific MAPs.

274 ochores in early mitosis and shuttles to the midzone spindle at mitotic exit.

275 nd separation, as well as recruitment of key midzone-stabilizing proteins all appeared normal, but mi

276 The spindle midzone stimulates the actomyosin-driven contraction of

277 that both actin binding and MKlp2-dependent midzone targeting cooperate to precisely position the CP

278 hBUBR1 localized to portions of the spindle midzone that did not overlap with CENP-E.

279 nterdigitating microtubules to establish the midzone that is necessary for cytokinesis.

280 Once at the midzone, the CYK-4 subunit functions to recruit actin re

281 It directs vesicles to the midzone, the site where they coalesce to form the new ce

282 f extra-long chromatids and clearance of the midzone, thereby avoiding cytokinesis failure and aneupl

283 tor composition of antiparallel ipMTs at the midzone, thereby facilitating the kinesin-5-driven slidi

284 isassembly factor to localize at the spindle midzone, thereby promoting spindle midzone dissolution.

285 yos, showing that Ect2 migrates from spindle midzone to astral microtubules in anaphase and that Ect2

286 antiparallel microtubules within the spindle midzone to play roles in bipolar spindle assembly and pr

287 over length scales of micrometres, from the midzone to the cell cortex, is not known.

288 egregate the chromosomes before anaphase and midzones to keep sister genomes apart and guide the clea

289 Also, it can localize to the midzone when other midzone components are depleted, sugg

290 d CHO1/MKLP1 is localized within the spindle midzone where it is thought to transport microtubules of

291 -2 to chromosomes and perhaps to the spindle midzone, where AIR-2 phosphorylates proteins that affect

292 h it congresses inward from the poles to the midzone, where it becomes progressively enriched at regi

293 se, the kinase Plk1 localizes to the spindle midzone, where it orchestrates cytokinesis.

294 ATK5 uses plus-end tracking to reach spindle midzones, where it then organizes microtubules via minus

295 ochores and, during anaphase, to the spindle midzone, whereas Kar3p-GFP was found at the nuclear side

296 Feo increases kinesin-5 association with the midzone, which becomes abnormally narrow, leading to imp

297 esis regulators that localize to the spindle midzone, which forms between the separating chromosomes.

298 ons activated Aurora B(AIR-2) at the spindle midzone, which is needed for the abscission checkpoint i

299 mmetric positioning of the telophase spindle midzone, which specifies the cleavage furrow.

300 hat it is essential for the integrity of the midzone, yet not for cytokinesis.