ライフサイエンスコーパス: minimal medium

1 soluble cruzain in high yields (>30 mg/L in minimal medium).

2 119 mutants were unable to grow on glycerol minimal medium.

3 erine during growth on glycerol and d-serine minimal medium.

4 ation and repressed when Fe(2+) was added to minimal medium.

5 eened in this study on glycerol-supplemented minimal medium.

6 Growth of S40 was severely impaired in minimal medium.

7 ins during chronic low-level AZT exposure in minimal medium.

8 growth in rich medium and host cells but not minimal medium.

9 t cannot use thymine for growth on a glucose minimal medium.

10 ant proteins in E. coli grown aerobically in minimal medium.

11 growing in conditioned complex medium or in minimal medium.

12 ion for Asp-tRNA(Asn) formation by growth in minimal medium.

13 h in the absence of thymine on LB but not on minimal medium.

14 mplex (Luria-Bertani [LB]) medium but not on minimal medium.

15 of cations or by a shift from rich to acidic minimal medium.

16 t not enough to support growth of E. coli in minimal medium.

17 orted the growth of the pfl mutant in xylose minimal medium.

18 RNA is increased at a low temperature and in minimal medium.

19 ent at the level of transcription in glucose minimal medium.

20 ficant lag in growth when diluted into fresh minimal medium.

21 tB during stationary phase in both broth and minimal medium.

22 hen grown in rich medium or in vir induction minimal medium.

23 651 mutant is viable on LB medium but not on minimal medium.

24 expressed in both ordinary and perdeuterated minimal medium.

25 ng 2,000 generations of evolution in glucose minimal medium.

26 tive to their Rbs(+) counterparts in glucose minimal medium.

27 9 yeast was analyzed for growth and color on minimal medium.

28 s when grown on rich (Luria-Bertani) than on minimal medium.

29 they were provided as sole carbon sources in minimal medium.

30 n initiation defects that are exacerbated in minimal medium.

31 ained constant and high throughout growth in minimal medium.

32 domain expresses at high yield (20 mg/l) in minimal medium.

33 um, such as Luria-Bertani medium, versus the minimal medium.

34 erichia coli strain SAB11 in iron-restricted minimal medium.

35 uantitative growth defects in either rich or minimal medium.

36 more slowly than wild-type cells in glucose minimal medium.

37 ties when cultured in rich medium or glucose-minimal medium.

38 th defect of the E. coli sodA sodB strain in minimal medium.

39 the enzyme, as judged by growth on succinate minimal medium.

40 tein is present by 2 h after suspension in N minimal medium.

41 ures in secY40 cells grown in either rich or minimal medium.

42 tein export defects in secY40 cells grown in minimal medium.

43 imal medium relative to its level in glucose minimal medium.

44 g growth in Luria-Bertani medium and glucose minimal medium.

45 rosmotic medium, and one failed to grow in a minimal medium.

46 gh wild type (arg-13+) is not derepressed in minimal medium.

47 in a chemically defined rich medium or in a minimal medium.

48 ption of mrgA-lacZ even in iron-supplemented minimal medium.

49 splay increased DCS resistance when grown in minimal medium.

50 sensitivity when strains are grown in a non-minimal medium.

51 urthermore, exhibit slow growth in Edinburgh minimal medium.

52 nce, with an upper limit of 225 mosmol/kg in minimal medium.

53 wild-type Escherichia coli growth on glucose minimal medium.

54 hways that are active in LB broth but not in minimal medium.

55 ellular pH of 7 than after growth at pH 5 in minimal medium.

56 well as by submerged mycelia grown in liquid minimal medium.

57 in a population of log phase cells grown in minimal medium.

58 attTn7 sites can be obtained by selection on minimal medium.

59 the chvD mutant was reduced in rich, but not minimal, medium.

60 ift-down from glucose amino acids to glucose minimal medium; (2) carbon source starvation after a "gl

61 In minimal medium, 30-40 mg of M7FP were obtained per liter

62 atis LR222 on iron-limiting (0.1 micro M Fe) minimal medium agar fluoresce under UV light due to the

63 Z expression was also observed in unbuffered minimal medium and appeared to be exerted posttranslatio

64 ast, CPA24 neither grows nor is killed in M9 minimal medium and artificial urine.

65 copy of fhs restored its ability to grow in minimal medium and at acidic pH as well as to produce mu

66 4-kilobase (kb) transcript is synthesized in minimal medium and both a 1.4- and 1.7-kb transcript are

67 Mutations in arg-13 result in slow growth in minimal medium and can suppress mutations in carbamyl ph

68 te secretome of A. nosocomialis strain M2 in minimal medium and demonstrate that pathogenic Acinetoba

69 ent growth defect in either nutrient-rich or minimal medium and no measurable virulence phenotype.

70 tions: high salt, sorbitol, galactose, pH 8, minimal medium and nystatin treatment.

71 educed cell size of PssB728a when grown in a minimal medium and on plant surfaces, while most ppGpp(0

72 ion in P. multocida in response to growth in minimal medium and provide a strong foundation to invest

73 ructose minimal medium but that in plant oil minimal medium and rich medium, phaB3 seems to be unexpr

74 at GadW represses the decarboxylase genes in minimal medium and that growth under acidic conditions l

75 ever, Deltaptc4 cells grow slowly in defined minimal medium and undergo premature growth arrest in re

76 e when this strain in cultivated in low-iron minimal medium, and a comparison of the growth character

77 ll appears to be optimal for rapid growth in minimal medium, and the gltBDF control region is designe

78 693 relA spoT double mutant for growth on M9 minimal medium, and the transformed cells produced rel(B

79 se protein, required glutamate for growth in minimal medium, and was unable to sporulate efficiently

80 features of E. coli cells growing on glucose minimal medium appeared to be the formation and excretio

81 Acid induction during log-phase growth in minimal medium appears to occur through multiple pathway

82 l (plasmolysis) titrations of cells grown in minimal medium at 0.03 Osm reduce cytoplasmic and cell w

83 ild-type and MG1655(rluA-) either in rich or minimal medium at 24, 37, or 42 degrees C, but when both

84 letion mutant of E. coli, allowing growth in minimal medium at 44 degrees C.

85 lpxP triple mutant, likewise grows slowly on minimal medium at all temperatures but not on nutrient b

86 and lpxL lpxM double mutants grow slowly on minimal medium at all temperatures, they do not grow on

87 rich medium at high induction levels and in minimal medium at low induction levels.

88 10b resulted in poor growth of the mutant in minimal medium at near the optimal growth temperature bu

89 Using minimal-medium batch and chemostat cultures, we comprehe

90 on 0.3% agar motility plates and in rich and minimal medium broth.

91 onditional: it can be maintained in cells in minimal medium but cannot be established in cells growin

92 ant had decreased growth in glucose-limiting minimal medium but grew normally when excess glucose was

93 cell envelope when the organism is grown in minimal medium but is secreted during growth in nutrient

94 /E57G and K31N/A50G/L218R) allowed growth on minimal medium but resulted in thiamine auxotrophy when

95 3 contribute to PHB biosynthesis in fructose minimal medium but that in plant oil minimal medium and

96 t extract-peptone complex medium and glucose minimal medium but was incapable of growth on glycerol.

97 face when E. coli O157:H7 was cultured in M9 minimal medium but were expressed from only a proportion

98 utes to E. coli biofilm formation in glucose-minimal medium, but not in Luria-Bertani broth.

99 from cultures grown in an hrp gene-inducing minimal medium by immunodetection with a DspE-specific a

100 rongly induced in fructose- or glucose-based minimal medium by L-phenylalanine.

101 abolic and regulatory programs of E. coli in minimal medium by reaction with homocysteine and cystein

102 In minimal medium, CFT073 dsdX can grow on d-serine as a so

103 ly 3-fold increase was seen during growth in minimal medium compared with rich medium.

104 e mid-logarithmic phase on acidified glucose minimal medium, conditions that induce glutamate-depende

105 asite gene restores growth of this mutant on minimal medium, confirming that the protein has IMPDH ac

106 own on enriched medium, while cells grown on minimal medium contained at various levels all c-type cy

107 on caused a growth defect at 45 degrees C in minimal medium containing 0.2 M NaCl that was similar to

108 In N minimal medium containing 10 mM Mg2+, all strains grew a

109 iphosphates isolated from E. coli grown in a minimal medium containing 12C, 14N-enriched glucose and

110 xS isogenic mutant of PAO1 did not grow in a minimal medium containing 2-ketogluconate as the sole ca

111 Cells produced (14)CO(2) from minimal medium containing [(14)C]adenine, which implies

112 Mxr1p is cytosolic in cells cultured in minimal medium containing a yeast nitrogen base, ammoniu

113 four- to sevenfold when cells were grown in minimal medium containing alanine or leucine.

114 , and JM101 allowed these strains to grow on minimal medium containing d-glucosaminate as the sole ca

115 ESR spectra of C. neoformans 24067 grown in minimal medium containing either 1.0 mM catechol, dopami

116 e altered if the enzyme was overexpressed in minimal medium containing Fe and Mn, and surprisingly, a

117 During rapid growth in minimal medium containing glucose and amino acids, CodY

118 and exhibited a bald phenotype when grown on minimal medium containing glucose as carbon source.

119 ae cells was explored in batch cultures on a minimal medium containing glucose as the sole carbon sou

120 rtant during exponential growth in acidified minimal medium containing glucose but were unnecessary f

121 tant E. coli by growing transformed cells on minimal medium containing glucuronic acid.

122 ch medium, AldA was required for growth on a minimal medium containing L-alanine as the major source

123 ressors of the cbr mutant were selected from minimal medium containing l-arginine as the sole carbon

124 trains lacking Rv1422 have growth defects in minimal medium containing limiting amounts of several di

125 the mig-14 promoter is induced by growth in minimal medium containing low magnesium or acidic pH, co

126 ects in the DeltapylT strain were evident in minimal medium containing methanol.

127 ved in comparison with that of the mutant in minimal medium containing mucin but not in the absence o

128 the fadD5 mutant was diminished in growth in minimal medium containing mycolic acid but not other lon

129 fragment conferred the capacity to grow on a minimal medium containing only 10 mM K+.

130 es measured in two media are necessary: in a minimal medium containing only derivatized metabolites a

131 mutant in this fungus that fails to grow in minimal medium containing polyamines as the sole nitroge

132 2-heptyl-3-hydroxy-4(1H)-quinolone (PQS), a minimal medium containing PQS as the sole carbon source

133 n in lacR mutants was seen in cells grown in minimal medium containing succinate and raffinose and gr

134 tial DHPS mutants were selected by growth on minimal medium containing the inhibitory lysine analogue

135 B. subtilis is unable to grow in minimal medium containing the iron chelator EDDHA unless

136 e-overproducing derivatives were selected on minimal medium containing the toxic proline analog 3,4-d

137 the iscS(-) strain is switched from rich to minimal medium containing thiamin and nicotinate, growth

138 sion and growth of P. pastoris cultured in a minimal medium containing yeast nitrogen base and methan

139 lx2) was overexpressed in rich medium and in minimal medium containing zinc, iron, or cobalt, and the

140 shed cells, inoculated at low density into a minimal medium, could not grow in its absence.

141 produced wild-type levels of cephamycin C in minimal medium culture conditions supplemented with lysi

142 s of the fhs mutant grown anaerobically in a minimal medium demonstrated that the mutant had an absol

143 Anaerobic induction in minimal medium depends strongly on FNR but is also affec

144 i host strain suspended in 5TFI-supplemented minimal medium depleted of isoleucine.

145 fied effector proteins secreted into defined minimal medium designed to induce expression of the SPI-

146 late ammonia as a sole source of nitrogen in minimal medium devoid of organic nitrogen sources.

147 Growth in GM medium plus cAMP or glycerol minimal medium did not result in a significant increase

148 ld type, was outcompeted by the wild type in minimal medium, displayed reduced swimming and swarming

149 lexigens is able to grow up to 3 m NaCl in a minimal medium due to the de novo synthesis of ectoines.

150 DH10B requires leucine for growth on minimal medium due to the deletion of leuLABCD and harbo

151 acetylglutaminylglutamine amide (NAGGN) in a minimal medium, due possibly to NAGGN synthesis depletin

152 in cells growing in steady state in glucose minimal medium, either in the presence or absence of leu

153 o effect on citB expression during growth in minimal medium even when combined with ccpC and abrB mut

154 phenazine biosynthetic gene transcription in minimal medium even when quorum-sensing signals were at

155 cid reported previously unexpectedly grew on minimal medium following transductional introduction of

156 of Escherichia coli propagated in a glucose minimal medium for 20,000 generations.

157 uction in cell mass by shifting to succinate minimal medium for inductions of the S105 gene.

158 ndant and/or share similar functions: (i) on minimal medium GreA overproduction suppresses the growth

159 mance of both models on predicting empirical minimal medium growth data/essential gene listings.

160 sed by reducing secondary initiation through minimal medium growth.

161 rage (19-fold) in continuous-flow cells with minimal medium (growth rate not altered and biofilm rest

162 imulates gdhA transcription during growth in minimal medium has been proposed to be the K. pneumoniae

163 zyme purified from the same E. coli grown in minimal medium has no color.

164 owth of the A1A2-deficient (A1A2-) mutant in minimal medium, implying that lipoate-dependent metaboli

165 complete medium is greater than that in the minimal medium in at least one peak, then unchanged D is

166 This mutant cannot grow in minimal medium in the absence of riboflavin supplementat

167 d simply upon switch from a rich medium to a minimal medium in the complete absence of nitrogen starv

168 guishable from the wild type when grown with minimal medium in the light and contained wild-type leve

169 argR mutant strain PAO501 grown in glutamate minimal medium in the presence and absence of arginine.

170 arent strain, the mutant failed to grow on a minimal medium in which 2-ketogluconate was the sole car

171 of wild-type M. catarrhalis was observed in minimal medium in which arginine was present only in met

172 19 conferred its greatest advantage in basic minimal medium in which either glucose or Casamino Acids

173 Unexpectedly, however, when grown in MOPS minimal medium, in mixed cultures, more hmp mutant cells

174 P1a or LpoA exhibited growth deficiencies in minimal medium, in the presence of deoxycholate and bile

175 exhibited several growth defects in glucose minimal medium, including reduced rates of growth and gr

176 nd citZ was derepressed in glucose-glutamine minimal medium, indicating that CcpC is a negative regul

177 ght major extracellular proteins (EXPs) in a minimal medium inducing hrp genes.

178 iments, wild-type yeast incubated in expired minimal medium instead of water lost viability quickly;

179 by chelators or a shift from rich to acidic minimal medium is largely dependent on functional EnvZ.

180 LpxC purified from Escherichia coli grown in minimal medium is mainly Fe(II).

181 f Chlamydomonas reinhardtii does not grow on minimal medium, is high light-sensitive under photoheter

182 When PA14 is grown on minimal medium, killing occurs over the course of severa

183 For cells grown in minimal medium, L12 acetylation and processing is altere

184 evisiae, is essential for growth in low iron minimal medium lacking citric acid.

185 e synthetases (DeltarelAPQ) does not grow in minimal medium lacking the branched-chain amino acids (B

186 In minimal medium, loss of PBPs 2c and 4 resulted in a slig

187 For cells in a minimal medium, Lrp levels were consistently lowest duri

188 smolalities (1.02-2.17 Osm) in MOPS-buffered minimal medium (MBM) containing 1 mM betaine (MBM+GB).

189 ni (LB) (0.07 muW/cm(2)) or a defined growth minimal medium (MM) (0.02 muW/cm(2)).

190 Genes with increased expression in minimal medium (n = 230) included those encoding amino a

191 llular fitness, measured as growth rate in a minimal medium, of UVR-exposed lineages of both B86-17 a

192 ly the outer membrane porin OmpU, whereas in minimal medium, OmpT is the dominant porin.

193 ually and other genes required for growth in minimal medium or for sporulation.

194 ive analysis of 12 hvKP strains in iron-poor minimal medium or human ascites fluid showed a significa

195 of the mutant to grow on glucose-containing minimal medium or increase its growth rate in the presen

196 argely suppressed when strains were grown on minimal medium or sucrose-free R2YE, where division site

197 nsate for the loss of both FtsL and DivIC on minimal medium or sucrose-free R2YE.

198 Remarkably, Escherichia coli grows in minimal medium over a wide range of external osmolalitie

199 We also show that minimal medium, pH 4.5, induces SPI-2 gene expression in

200 In minimal medium, phtA mutants do not replicate; in rich b

201 When grown in minimal medium plus acetate (McA), all ehb mutants had s

202 Yeast grown in minimal medium plus lysine show significant reductions i

203 determined, with the slower-growing cells in minimal medium possessing fewer of the individual PBPs p

204 H. capsulatum mycelia in chemically defined minimal medium produced pigmented conidia.

205 with a six-histidine tag using an optimized minimal-medium protocol for subsequent purification.

206 Surprisingly, growth in minimal medium reduced the ability of seqA+ strains to f

207 MP (cAMP) and was elevated also in succinate minimal medium relative to its level in glucose minimal

208 A minimal medium required by the model to generate require

209 mol/OD460 in glucose amino acids and glucose minimal medium, respectively, to about 100 pmol ppGpp/OD

210 However, the addition of Casamino Acids to minimal medium results in a dose-dependent decrease in h

211 growth and fast swimming, while evolution in minimal medium results in fast growth and slow swimming.

212 Batch culture experiments on minimal medium revealed that LSG184 grows well on a vari

213 ated for thousands of generations in glucose minimal medium show heritable increases in both cell siz

214 The cells grown on minimal medium showed significantly elevated expression

215 utilization (hut) enzymes in cells grown in minimal medium showed that neither the ptsH1 nor the crh

216 ll mutation in nac displayed growth rates on minimal medium similar to the wild type.

217 of a (p)ppGpp(0) Escherichia coli strain in minimal medium, SMG and on medium containing 3-amino-1,2

218 The use of a minimal medium sufficient for hESC growth is expected to

219 tive in motility, luminescence, or growth in minimal medium, suggesting that it lacks an essential, p

220 grew well in rich medium but did not grow in minimal medium supplemented by arginine and nucleosides.

221 ged exponential growth was performed with M9 minimal medium supplemented with 2 g of alpha-ketoglutar

222 restored growth of an rcnA mutant in glucose minimal medium supplemented with 4 microM Ni(II), thus c

223 ient labeling of riboses following growth in minimal medium supplemented with [2-(13)C]acetate.

224 Fpg was also expressed in minimal medium supplemented with cobalt nitrate to yield

225 ne in M. smegmatis could be achieved only in minimal medium supplemented with D-glutamate, demonstrat

226 These mutants displayed attenuated growth in minimal medium supplemented with glucose as the sole car

227 presses Vibrio cholerae biofilm formation in minimal medium supplemented with glucose or pyruvate.

228 5 inner colony mutants had reduced growth on minimal medium supplemented with glucose-6-phosphate.

229 get found to be regulated by GcvB in glucose minimal medium supplemented with glycine.

230 sstT-lacZ in Luria-Bertani broth and glucose minimal medium supplemented with glycine.

231 about threefold when the cells were grown in minimal medium supplemented with leucine.

232 An isogenic zwf mutant was unable to grow on minimal medium supplemented with mannitol.

233 eleterious to the growth of a prp+ strain on minimal medium supplemented with propionate as a carbon

234 'leaky' growth phenotype on aerobic glucose minimal medium supplemented with succinate (which bypass

235 tants were unable to grow on 1,2-propanediol minimal medium supplemented with vitamin B(12) but were

236 in dilute rich soft-agar swarm medium or in minimal-medium swarm plates containing any 1 of 60 chemo

237 hich was consistently higher in hrp-inducing minimal medium than in nutrient-rich Luria-Bertani broth

238 expressed at significantly higher levels in minimal medium than in rich medium, and rpoS expression

239 re 3- to 4-fold higher in cells growing in a minimal medium than those in a rich medium.

240 ; the mutant showed a reduced growth rate in minimal medium that could be reversed by the addition of

241 s, and (ii) a selective advantage in glucose minimal medium that drove these mutants to fixation.

242 istoplasma genome and confirmed by growth in minimal medium that Histoplasma yeasts can synthesize al

243 When grown in minimal medium, the majority (approximately 89%) of the

244 omplete and homogeneous in all cases, but in minimal medium, the periplasm is evidently thicker at th

245 ubations were carried out in iron-containing minimal medium, the products formed colored chelates.

246 During growth in minimal medium, the ptsH1 mutation, which prevents seryl

247 nstrate that, during R. capsulatus growth on minimal medium, the requirement for CycH in c-type cytoc

248 grees C and FH12 lacking the plasmid grew on minimal medium, thereby establishing that idi is a nones

249 e enzyme were overexpressed in perdeuterated minimal medium to allow detection and assignment of prot

250 ic growth, growth on paraquat, and growth on minimal medium to an Escherichia coli (Ec) mutant defici

251 is drug after nutritional shiftup, e.g. from minimal medium to Luria broth.

252 In E. coli cells grown in minimal medium to mid-log phase, the ratio of free to DN

253 In defined minimal medium, transcription of cspA, -B, -G, and -I wa

254 o model realistic cells growing in a glucose minimal medium under aerobic conditions.

255 tyl phosphate, also failed to grow in xylose minimal medium under anaerobic conditions, confirming th

256 rmed optimally when bacteria were grown in a minimal medium under anaerobic conditions.

257 inhibits cell growth of Escherichia coli in minimal medium under anaerobic growth conditions and tha

258 ts were generated and screened for growth on minimal medium under high CO(2) conditions (5% CO(2) in

259 the class II mutants were unable to grow on minimal medium unless a complex mixture of amino acids w

260 es the aconitase prevented growth in glucose minimal medium unless heme or succinate was added to the

261 prevented the growth of Escherichia coli in minimal medium unless the medium was supplemented with a

262 hore membranes obtained using cells grown in minimal medium was decreased to approximately 50%.

263 ntent in both rich Luria broth medium and M9 minimal medium was determined, with the slower-growing c

264 Growth of this mutant in minimal medium was inhibited by the aryl acids, but the

265 Acid resistance of the fur mutant in minimal medium was restored by addition of glutamate dur

266 In minimal medium, we detected transcripts for approximatel

267 re evolved for 50,000 generations in glucose minimal medium, we observed modest changes in relative f

268 ation is predominant when cells are grown in minimal medium, we propose that these modifications form

269 that allow growth of lipB strains on glucose minimal medium; we determined that suppression was cause

270 cspE mRNA levels in defined minimal medium were drastically higher than mRNA for the

271 B levels in Bacillus subtilis cells grown in minimal medium were increased approximately 7-fold by GB

272 on the growth conditions; lysogens grown in minimal medium were nearly stable but switched at high r

273 In minimal medium where phenazine production is very low, i

274 ter is knocked out showed impaired growth in minimal medium where the only source of arginine came fr

275 When parallel studies were conducted in minimal medium, where the mutD5 strain is defective in e

276 However, the wild type self-aggregates in minimal medium, whereas the quorum-sensing mutant does n

277 Bacillus tequilensis cells grown in a minimal medium with (15)NH4Cl as the nitrogen source wer

278 The supplementation of a minimal medium with a mixture of asparagine, arginine, g

279 A gdhA mutant was growth deficient in minimal medium with citrate as the sole carbon source, a

280 CFT073 cycA can grow on minimal medium with d-serine as a sole carbon source.

281 s to restore growth of a Deltamdh2 strain on minimal medium with ethanol or acetate as the carbon sou

282 E. coli cells were grown in minimal medium with fixed final concentrations of carbon

283 rains demonstrated identical growth rates in minimal medium with glucose as the carbon source.

284 nstrable PckA enzyme activity and grew on AB minimal medium with glucose but did not grow on the same

285 Such mutants grew well in minimal medium with glutamate as the sole nitrogen sourc

286 e of Escherichia coli strain MG1655 grown in minimal medium with glycerol as the carbon source.

287 not only by serum but also during growth in minimal medium with glycerol as the sole carbon source a

288 of H. capsulatum yeast in chemically defined minimal medium with L-3,4-dihydroxyphenylalanine (DOPA)

289 The ald mutants grew poorly in minimal medium with L-alanine as the sole nitrogen sourc

290 was also eliminated upon supplementation of minimal medium with serine or glycine for solid medium o

291 was also shown to restore growth to EV78 in minimal medium with sialic acid as the sole carbon sourc

292 ring a five-week time course the MC grown in minimal medium with sugarcane bagasse (SCB) as a sole ca

293 e MSDH activity and the ability to grow in a minimal medium with valine or isobutyrate as the sole ca

294 mgtC+ mgtB strain was also able to grow in N minimal medium without added Mg2+ but only after a 24-h

295 nd mgtC+ mgtB+ strains exhibited growth in N minimal medium without added Mg2+ with a 1- to 2-h lag p

296 lecting for growth of cells lacking ppGpp on minimal medium without amino acids.

297 to grow under anaerobic conditions in xylose minimal medium without any negative effect on their surv

298 allowing growth on 300 microm 5MT-containing minimal medium without tryptophan, and cell extracts con