ライフサイエンスコーパス: minus-end-directed

1 o which the motor domain binds, KlpA becomes minus end-directed.

2 s KlpA without the tail is nonprocessive and minus end-directed.

3 s-end directed molecular motor, while ncd is minus-end directed.

4 Myosin VI (Myo6) is a minus end-directed actin-based motor found in neurons th

5 a yeast two-hybrid screen, we identified the minus-end-directed actin-based motor, myosin VI, as an S

6 irected activity of cytoplasmic dynein and a minus end-directed activity associated with NuMA during

7 end-directed activity of Eg5 antagonizes the minus end-directed activity of cytoplasmic dynein and a

8 dhc-1 and unc-116, components of the dynein minus-end-directed and kinesin-1 plus-end-directed micro

9 makes the dynamics of Ncd non-processive and minus-end directed as opposed to kinesin-1.

10 s, is specifically altered; net transport is minus-end directed at developmental stages when it is no

11 Nonclaret disjunctional (Ncd) is a minus end-directed, C-terminal motor protein that is req

12 dynein is a molecular motor responsible for minus-end-directed cargo transport along microtubules (M

13 Cytoplasmic dynein is the major microtubule minus-end-directed cellular motor.

14 Motor proteins of the minus end-directed cytoplasmic dynein and plus end-direc

15 Live-cell imaging reveals minus end-directed dynein-dynactin motility along microt

16 us ends, suggesting that NUDF may facilitate minus-end-directed dynein movement.

17 pronucleus, while simultaneously generating minus-end directed flows along MTs that are similar to t

18 combination of two aggregate properties, Net Minus-end-directed Force and microtubule Cross-linking O

19 Kar3Cik1 then uses its minus-end-directed force to depolymerize microtubules fr

20 Kinesin-14 motors generate microtubule minus-end-directed force used in mitosis and meiosis.

21 or inhibitors of autophagy or by modulating minus-end-directed (HDAC6 shRNA) or plus-end-directed (R

22 LIS1) in the interaction of end tracking and minus end-directed human dynein complexes with these sit

23 are the first to localize the activity of a minus end-directed kinesin at the plus ends of microtubu

24 3 protein from Saccharomyces cerevisiae is a minus end-directed kinesin family member that is involve

25 This sliding was mediated by the minus end-directed kinesin motor Klp2, which helped micr

26 We find that Kar3p, a minus end-directed kinesin motor protein, is required, w

27 ns (KCHs) belong to a distinct branch of the minus end-directed kinesin subfamily.

28 uclear positioning via inhibition of Klp2, a minus end-directed kinesin-14.

29 r with a depolymerase (Kip3, class Kin-8) or minus-end-directed kinesin (Kar3, class Kin-14), can sup

30 nteraction between the Kar3 motor protein, a minus-end-directed kinesin from yeast, and its associate

31 tween yellow fluorescent protein (YFP) and a minus-end-directed Kinesin-14 (C-terminal family) from A

32 st, merotely is increased by deletion of the minus-end-directed kinesin-14 (Klp2) or overexpression o

33 We found that perturbation of the minus-end-directed Kinesin-14 HSET increased the frequen

34 the role of tetrameric Kif25, a microtubule minus-end-directed kinesin-14 motor, in preventing prema

35 analysed the motility of the six members of minus-end-directed kinesin-14 motors in the moss Physcom

36 Kar3, a Saccharomyces cerevisiae microtubule minus-end-directed kinesin-14, dimerizes with either Vik

37 catalytic core, a signature neck peptide of minus end-directed kinesins, and a unique calponin homol

38 a C-terminal motor domain contains all known minus end-directed kinesins.

39 Myosin VI, an actin-dependent, minus-end directed mechanoenzyme, has been implicated in

40 y, indicating that dynein is the predominant minus end-directed membrane motor in Xenopus egg extract

41 related well with the eightfold reduction in minus end-directed membrane transport observed in metaph

42 The minus end-directed microtubule motor cytoplasmic dynein

43 The minus end-directed microtubule motor cytoplasmic dynein

44 Cytoplasmic dynein is the major minus end-directed microtubule motor in eukaryotes.

45 Genetic studies imply that the minus end-directed microtubule motor kinesin-like calmod

46 , using proteomic profiling, we identify the minus end-directed microtubule motor protein HSET as a d

47 e calmodulin (CaM) binding protein (KCBP), a minus end-directed microtubule motor protein unique to p

48 Cytoplasmic dynein 1 (dynein) is a minus end-directed microtubule motor protein with many c

49 To test the hypothesis that the minus end-directed microtubule motor protein, cytoplasmi

50 Non-claret disjunctional protein (Ncd) is a minus end-directed microtubule motor required for normal

51 Cytoplasmic dynein is a minus end-directed microtubule motor that performs disti

52 Dynein is a minus end-directed microtubule motor that transports num

53 Cytoplasmic dynein is a multisubunit, minus end-directed microtubule motor that uses dynactin

54 Cytoplasmic dynein is an enormous minus end-directed microtubule motor.

55 esin-14s are commonly known as nonprocessive minus end-directed microtubule motors that function main

56 Therefore, myoJ cooperates with plus and minus end-directed microtubule motors to drive the norma

57 abE engage myosin-5 as well as plus end- and minus end-directed microtubule motors, providing an expe

58 hosphate (GTP)-Rab7 effectors that instigate minus end-directed microtubule transport.

59 Targeting of the minus-end directed microtubule motor cytoplasmic dynein

60 ) component of dynactin, an activator of the minus-end directed microtubule motor dynein; an associat

61 osition depends on a balance of plus-end and minus-end directed microtubule motor function.

62 Kar3 is a minus-end directed microtubule motor involved in meiosis

63 The minus-end directed microtubule motor protein cytoplasmic

64 1, the heavy chain of the cytoplasmic dynein minus-end directed microtubule motor, in a genetic scree

65 organelle in possession of both plus-end and minus-end directed microtubule motors and a myosin; coor

66 Cytoplasmic dynein is the major minus-end directed microtubule-based motor in eukaryotic

67 Cytoplasmic dynein is a minus-end directed microtubule-based motor.

68 Cytoplasmic dynein is the predominant minus-end-directed microtubule (MT) motor in most eukary

69 ubules from the plus end and promotes robust minus-end-directed microtubule gliding.

70 Ncd is a minus-end-directed microtubule motor and a member of the

71 Dynactin is a required cofactor for the minus-end-directed microtubule motor cytoplasmic dynein.

72 HIP) (FHF) complex, which interacts with the minus-end-directed microtubule motor dynein and its acti

73 s cytoplasmic dynein is primarily known as a minus-end-directed microtubule motor for organelle trans

74 Dynein is a minus-end-directed microtubule motor important for mitot

75 Cytoplasmic dynein is the primary minus-end-directed microtubule motor protein in animal c

76 arrays in dividing cells suggests that this minus-end-directed microtubule motor protein is likely t

77 Kar3 is a minus-end-directed microtubule motor that is implicated

78 Cytoplasmic dynein is a minus-end-directed microtubule motor that participates i

79 Cytoplasmic dynein is a multisubunit minus-end-directed microtubule motor that serves multipl

80 Cytoplasmic dynein is a minus-end-directed microtubule motor whose mechanism of

81 Cytoplasmic dynein, a minus-end-directed microtubule motor, has been implicate

82 Cytoplasmic dynein, a minus-end-directed microtubule motor, has been implicate

83 behavior of cytoplasmic dynein, the primary minus-end-directed microtubule motor.

84 oteins (KLPs) are a large family of plus- or minus-end-directed microtubule motors important in intra

85 nd by extension those of intact nuclei, bear minus-end-directed microtubule motors.

86 lasmic dynein is responsible for most of the minus-end-directed microtubule traffic within cells.

87 tin, a complex that functions with dynein in minus-end-directed microtubule transport.

88 termittent movement involving both plus- and minus-end-directed microtubule-based motilities in the p

89 ctin are regulators of cytoplasmic dynein, a minus end-directed, microtubule (MT)-based motor require

90 abeling revealed the attachment of dynein, a minus end-directed, microtubule-dependent motor, to the

91 y deletions in any or all of this organism's minus end-directed, microtubule-dependent motors: two re

92 x that is required for cytoplasmic dynein, a minus-end-directed, microtubule-associated motor, to eff

93 r cytoplasmic dynein is responsible for most minus-end-directed, microtubule-based transport in eukar

94 nsisting of the plus-end tracker EB1 and the minus-end-directed molecular motor Kinesin-14 is suffici

95 ingle microtubules, but reverts to canonical minus end-directed motility when anchored on the surface

96 rked MTs showed approximately equal plus and minus end-directed motility, immunofluorescence microsco

97 whether the mechanism of the unconventional minus-end-directed motility differs fundamentally from t

98 ing of the structural changes underlying the minus-end-directed motility of Kinesin-14 motors (such a

99 y-conserved catalytic core, is essential for minus-end-directed motility, as mutagenesis of these nec

100 ytoplasmic dynein, the 1.2 MDa motor driving minus-end-directed motility, has been reported to move p

101 e (MT) motor protein responsible for most MT minus-end-directed motility.

102 is controlling the switch between plus- and minus-end-directed motility.

103 Kip2, helping it overcome dynein's intrinsic minus-end-directed motility.

104 fferent membranes promotes their microtubule minus-end-directed motility.

105 on the balance between plus-end directed and minus-end directed motion.

106 ndrites indicate that models suggesting that minus end-directed motor activity is sufficient for dend

107 Cytoplasmic dynein is a large minus end-directed motor complex with multiple functions

108 r to translocation of the MTOC, in which the minus end-directed motor cytoplasmic dynein, localized a

109 Cell biologists have long speculated that a minus end-directed motor localized at kinetochores contr

110 mobilized on microtubules by the activity of minus end-directed motor proteins that interact either d

111 ted with centrosomal microtubules (C-MTs) by minus end-directed motor proteins.

112 plus end-directed motor, kinesin-II, and the minus end-directed motor, cytoplasmic dynein in highly p

113 at links cargos of aggregated protein to the minus end-directed motor, cytoplasmic dynein.

114 Cytoplasmic dynein is the major microtubule minus end-directed motor.

115 We find that while the minus-end directed motor Dynein cooperates with Dynactin

116 Drosophila Ncd, the other well characterized minus-end directed motor that is a homodimer, Kar3 is a

117 ve studied the shape of myosin VI, the actin minus-end directed motor, by negative stain and metal sh

118 formation of aggresomes, suggesting that the minus-end-directed motor activities of cytoplasmic dynei

119 es, where it then organizes microtubules via minus-end-directed motor activity.

120 along microtubules by using the microtubule minus-end-directed motor complex of dynein/dynactin.

121 dynein/dynactin, a multi-subunit microtubule minus-end-directed motor complex, and NuMA, a microtubul

122 Our data reveal the novel finding that a minus-end-directed motor contributes to the organization

123 Instead, we found that mutations in the minus-end-directed motor cytoplasmic dynein, completely

124 While it is clear that the minus-end-directed motor dynein is required for this pro

125 Inhibitory antibodies indicated that the minus-end-directed motor dynein is required to prevent p

126 the transport of a subset of cargoes by the minus-end-directed motor dynein, although the full exten

127 in that mediates linkage of cargoes with the minus-end-directed motor dynein.

128 Cytoplasmic dynein is the microtubule minus-end-directed motor for the retrograde axonal trans

129 cortex required Bud6p, Bim1p, and dynein, a minus-end-directed motor helping tether the receding plu

130 involved in organelle transport) and ncd (a minus-end-directed motor involved in chromosome segregat

131 ge, is transported to the nucleus via the MT minus-end-directed motor protein dynein.

132 a wide range of cellular functions for this minus-end-directed motor protein.

133 Kar3 is the minus-end-directed motor protein; Cik1 binds to Kar3 and

134 g microtubules, driven by teams of plus- and minus-end-directed motor proteins.

135 Cytoplasmic dynein is a microtubule minus-end-directed motor that is thought to power the tr

136 tor, the clustering of their minus ends by a minus-end-directed motor, and the loss of MTs by dynamic

137 mic dynein complex is an active, microtubule minus-end-directed motor, as expected.

138 motors containing the stalk and neck of the minus-end-directed motor, Ncd, fused to the motor domain

139 ative actions of the bipolar kinesin-5 and a minus-end-directed motor, which then pulls the sliding M

140 a gal suggests that the head of Nod may be a minus-end-directed motor.

141 d simulations, we propose a model on how two minus end-directed motors cooperate to ensure spindle po

142 ac-man model, in which 1) kinetochore-based, minus end-directed motors generate poleward forces for a

143 Likewise, minus end-directed motors may move cargoes toward anteri

144 ng of nuclear congression and identified two minus end-directed motors operating in parallel in this

145 Here, we have characterized the roles of two minus end-directed motors, dynein and Ncd, in such proce

146 T, the human homologue of the KAR3 family of minus end-directed motors.

147 ng, suggesting that they were transported by minus end-directed motors.

148 by the opposing activities of plus end- and minus end-directed motors.

149 ongression depends on the cooperation of two minus end-directed motors.

150 es (MTs) are substrates upon which plus- and minus-end directed motors control the directional moveme

151 Kinesin-14 family proteins are minus-end directed motors that cross-link microtubules a

152 bidirectionally, employing both plus-end and minus-end directed motors.

153 However, in the SAC model, minus-end-directed motors bind the minus ends of MTs as

154 se mitotic spindles with inactive kinesin-14 minus-end-directed motors often have shorter spindle len

155 -directed motors are balanced by forces from minus-end-directed motors that pull spindle poles togeth

156 rucial role in the coordination of plus- and minus-end-directed motors.

157 ytoskeleton has pointed instead to roles for minus-end-directed motors.

158 be created by complexes combining plus- and minus-end-directed motors.

159 d for a single step and force generation for minus-end directed movement generated by this non-proces

160 However, the functional necessity of minus-end-directed movement along actin is unclear as th

161 ergo either diffusive, or highly processive, minus end-directed movements along microtubules.

162 bule tracks proceeds in a series of plus and minus end-directed movements that are facilitated by kin

163 owed a specific decrease in the frequency of minus end-directed movements.

164 or motor protein responsible for microtubule minus-end-directed movements in most eukaryotic cells.

165 ated with RNA localization signals mediating minus end-directed mRNA transport during Drosophila deve

166 organization that requires the activity of a minus-end-directed MT motor, cytoplasmic dynein.

167 movements), which are commonly attributed to minus end-directed, MT-dependent motors.

168 we present cryo-EM structures of the unique minus-end directed myosin VI motor domain in rigor (4.6

169 lus end-directed kinesin heavy chain and the minus end-directed Ncd.

170 members; MT plus-end-directed kinesin and MT minus-end-directed non claret disjunctional (ncd).

171 ried out with monomeric motor domains of the minus-end-directed nonclaret disjunctional (Ncd) and Kar

172 the dynein heavy chain dramatically inhibits minus end-directed organelle transport and that purified

173 tively regulate interphase processes such as minus-end-directed organelle trafficking.

174 d dynactin in a search-capture mechanism for minus-end-directed organelles.

175 ecipitously shortly before transport becomes minus-end directed (phase III).

176 the rapid activation of dynein-dependent MT minus-end-directed pigment granule movement in Xenopus m

177 be consistent with the previously discovered minus-end directed power stroke of ncd, occurring with A

178 jacent microtubule protofilaments and uses a minus-end-directed powerstroke to drive ATP-dependent mo

179 imulates dynein motor activity and increases minus-end-directed runs of pigment granules.

180 ing the frequency, velocity, and duration of minus-end-directed runs.

181 We propose that the minus end-directed stepping action of Cut7 is selectivel

182 r domains with their neighbors inhibit their minus end-directed stepping action, but not their plus e

183 r Klp2, a kinesin-14, converts Cut7 from net minus end-directed to net plus end-directed stepping.

184 h greater crowding converting the motor from minus end-directed to plus end-directed stepping.

185 The major function of dynactin is minus end-directed transport along microtubules in a com

186 nucleus occurs through a well characterized minus end-directed transport along microtubules.

187 egation signals in melanophores stimulate MT minus end-directed transport by the increasing number of

188 This minus end-directed transport is largely unaffected by ge

189 bule-dependent motors, we establish that the minus end-directed transport of SVs requires the dynein/

190 decreases dynein binding to APC to stimulate minus end-directed transport, which could modulate endoc

191 capture membrane organelles destined for MT minus end-directed transport.

192 ificantly contribute to the efficiency of MT minus-end directed transport of membrane organelles.

193 otor that utilizes ATP hydrolysis to conduct minus-end directed transport of various organelles.

194 principal microtubule organizing center) by minus-end-directed transport and then switch polarity an

195 to threefold and at the same time decreased minus-end-directed transport of mitochondria along axona

196 ssembly and reassembly of MTs and concurrent minus-end-directed transport of pigment granules bearing

197 ndicating that this interaction mediates the minus-end-directed transport of the viral genome along m

198 ndria by disturbing the balance of plus- and minus-end-directed transport rather than directly affect

199 ons in Myo6, the gene encoding the (F-actin) minus end-directed unconventional myosin, myosin VI, cau

200 Cytoplasmic dynein drives the majority of minus end-directed vesicular and organelle motility in t