ライフサイエンスコーパス: misincorporate

1 ed low fidelity on template T, preferring to misincorporate a G instead of an A.

2 m, we demonstrate that RNA polymerase II can misincorporate a nucleotide and carry out template-depen

3 When bypass did occur, pol gamma misincorporated a guanine residue opposite the 3'-thymin

4 e C, and MutY DNA glycosylase, which removes misincorporated A opposite 8-oxoG.

5 The adenine glycosylase MutY, which removes misincorporated A residues from OG:A mismatches, is unab

6 -glycosylase and increased the efficiency of misincorporating A opposite the lesion by DNA polymerase

7 lta and yeast Pol eta both bypass 8-oxoG and misincorporate adenine during bypass.

8 ase) activity coupled with MutY's removal of misincorporated adenine bases was sought using both qual

9 the prevention of DNA mutations by removing misincorporated adenine residues from 7, 8-dihydro-8-oxo

10 of mutations associated with OG by removing misincorporated adenine residues from OG:A mismatches.

11 cision repair (BER) glycosylase that removes misincorporated adenine residues from OG:A mispairs, as

12 deoxyguanosine (OG) in DNA by the removal of misincorporated adenine residues in OG:A mispairs.

13 nine DNA glycosylase, can effectively remove misincorporated adenines opposite template G or 8-oxoG b

14 r T was 5' to the lesion; dAMP and dTMP were misincorporated at a frequency of 2-4%.

15 ability of extension after misalignment of a misincorporated base on the next (complementary) templat

16 elity; seven unique mutants that efficiently misincorporate bases and/or extend mismatched bases were

17 *); while mainly error-free, the identity of misincorporated bases is influenced by local sequence co

18 has a relatively low ability to extend from misincorporated bases, accounting for the low level of o

19 poration, and 3) proofreading by excision of misincorporated bases.

20 separate 3'-5' exonuclease activity to edit misincorporated bases.

21 The paradox of misincorporating both a smaller (glycine) and a larger (

22 y, epigenetic inheritance, rapid turnover of misincorporated CenH3, and transcriptional quiescence of

23 Backtracked/misincorporated complexes can be resolved via hydrolysis

24 ervation implies that physical resolution of misincorporated complexes may be the main function of th

25 TL by Gre factor occurs only in backtracked/misincorporated complexes, and not in correctly elongati

26 ells can be explained by the accumulation of misincorporated complexes, rather than mistakes in matur

27 s lacking proofreading factors are, in fact, misincorporated complexes.

28 8-oxodG, Fapy.dG instructs DNA polymerase to misincorporate dA opposite it in vitro.

29 The catalytic subunit alone primarily misincorporated dAMP and dGMP opposite the BaP DE-dG add

30 d K289M protein revealed that it was able to misincorporate dCTP opposite template C and dGTP opposit

31 , encodes the DNA polymerase Pol iota, which misincorporates deoxynucleotides at a high rate.

32 ate kinetic analyses and find that hPoltheta misincorporates deoxynucleotides with a frequency of abo

33 on indicate that pol eta has a low fidelity, misincorporating deoxynucleotides with a frequency of ab

34 Using this platform, we found misincorporated dNs occurring at 1 per 10(3) to 10(5) ri

35 ynthesizes DNA with extremely high fidelity, misincorporating dTMP, dAMP, and dGMP opposite a templat

36 REV1 misincorporated dTTP and dGTP with much lower frequencie

37 previously established that both polymerases misincorporated dTTP with high frequency across from cis

38 l A-family polymerases, is uniquely prone to misincorporating dTTP opposite template G in a highly se

39 nown to be able to hydrolyze ribonucleotides misincorporated during genomic replication.

40 4 glycosylase indicated that there was more misincorporated FU:Gua in the DNA of MMR-deficient HCT11

41 all four diol epoxide adducts, preferring to misincorporate G and A at frequencies 3- to more than 50

42 Rev1 misincorporates G, A, and T residues opposite template G

43 h error-free and mutant transcripts with AMP misincorporated immediately downstream from the lesion.

44 It enhances both cleavage of ribonucleotides misincorporated in DNA duplexes, and the comprehensive h

45 s, we have engineered C. albicans strains to misincorporate increasing levels of Leu at protein CUG s

46 iron does not normally bind SOD2, iron will misincorporate into Saccharomyces cerevisiae Sod2p when

47 om uracil, the latter accumulating and being misincorporated into DNA during folate depletion, the DN

48 Ribonucleotides are frequently misincorporated into DNA during replication, and they ar

49 , RNase H2 can remove single ribonucleotides misincorporated into DNA during replication.

50 g (MYH) is responsible for removing adenines misincorporated into DNA opposite guanine or 7,8-dihydro

51 f oxidative modification of dGTP, thatcan be misincorporated into DNA, causing AT-->CG mutations.

52 NA repair nuclease whose substrate is uracil misincorporated into DNA.

53 nome stability as it removes ribonucleotides misincorporated into genomic DNA by replicative polymera

54 Here, we show that fluorothreonine can be misincorporated into protein in place of the proteinogen

55 Ribonucleotides are misincorporated into replicating DNA due to the similari

56 gation as well as removal of incorrect bases misincorporated into RNA.

57 chromatography have suggested that uracil is misincorporated into the DNA of patients with megaloblas

58 eins contained sub-stoichiometric amounts of misincorporated lead and uranium.

59 In the absence of PCNA, pol delta misincorporates less than one nucleotide for every 100,0

60 ll of the analogues, even though this enzyme misincorporates natural NTPs at frequencies as high as 1

61 DNA polymerase makes errors by misincorporating natural DNA bases and base analogs.

62 Primase did not efficiently misincorporate NTPs during the initiation reaction (i.e.

63 lymerization revealed that human primase can misincorporate NTPs via both template misreading and a p

64 It misincorporated NTPs at frequencies as high as 1 in 7, a

65 Even though primase misincorporates NTPs at a relatively high frequency, thi

66 at the target was determined to be about one misincorporated nucleotide per 1900 repaired uracil resi

67 is stimulation is much higher in the case of misincorporated nucleotide.

68 ditionally, we found that herpes primase can misincorporate nucleotides both by misreading the templa

69 A polymerases are high fidelity enzymes that misincorporate nucleotides into nascent DNA with a frequ

70 an Poleta are low-fidelity enzymes, and they misincorporate nucleotides with a frequency of 10(-2)-10

71 es show that on undamaged DNA, both proteins misincorporate nucleotides with frequencies of approxima

72 t suppress genetic instability by correcting misincorporated nucleotides and insertion/deletion mispa

73 on by its polymerase activity and removal of misincorporated nucleotides by its exonuclease activity.

74 exonuclease activity that is used to remove misincorporated nucleotides from the nascent DNA (proofr

75 e non-Watson-Crick base pairs and excise the misincorporated nucleotides from the nascent DNA strand,

76 ease activity, pol II quantitatively removed misincorporated nucleotides from the nascent transcript

77 ta was relatively accurate, the human enzyme misincorporated nucleotides opposite the lesion with fre

78 ation, the hydrolytic reaction stimulated by misincorporated nucleotides proofreads most of the misin

79 ch repair (MMR) is responsible for detecting misincorporated nucleotides, resulting in excision repai

80 d are featured with 5'-monophosphates and 3'-misincorporated nucleotides.

81 nt 3'-->5' exonuclease activity that excises misincorporated nucleotides.

82 e DNA but also have the ability to proofread misincorporated nucleotides.

83 ll four nondamaged template bases, hPol(eta) misincorporates nucleotides with a frequency of approxim

84 The I260Q hinge variant of polymerase beta misincorporates nucleotides with a significantly higher

85 g (MYH) is responsible for removing adenines misincorporated on a template DNA strand containing G or

86 tion fidelity by removing adenines that were misincorporated opposite 7,8-dihydro-8-oxo-deoxyguanines

87 oving 8-oxoG directly or by removing adenine misincorporated opposite 8-oxoG or both.

88 tY homologue (MYH or MUTYH) removes adenines misincorporated opposite 8-oxoguanine as part of the bas

89 minating 3' to the adduct site contained AMP misincorporated opposite dC.

90 g (MYH) is responsible for removing adenines misincorporated opposite DNA strands containing guanine

91 osylase MutY homolog (Myh1) excises adenines misincorporated opposite guanines or 7,8-dihydro-8-oxo-g

92 OG-associated mutations by removing adenines misincorporated opposite OG lesions during DNA replicati

93 In addition, the dATP can be misincorporated opposite the adduct.

94 w that its mutator phenotype is specific for misincorporating opposite template A up to 6-fold more t

95 t group of base-excision enzymes that remove misincorporated or cytosine-derived uracil from nascent

96 ror correction involves the translocation of misincorporated products from the synthetic to the editi

97 mately 2-fold lower rates of excision of the misincorporated purine nucleotides opposite gamma-HOPdG

98 The transcripts containing misincorporated residues can be cleaved by the very slow

99 Replicative DNA polymerases misincorporate ribonucleoside triphosphates (rNTPs) into

100 ciently remove a tyrosine linked to a single misincorporated ribonucleotide or to polyribonucleotides

101 All DNA polymerases misincorporate ribonucleotides despite their preference

102 n mutagenic profile of DnaE, we propose that misincorporated ribonucleotides are removed by NER follo

103 Therefore, misincorporated ribonucleotides are targeted by the cell

104 Misincorporated ribonucleotides in DNA will cause DNA ba

105 roposed role of this enzyme in the repair of misincorporated ribonucleotides rather than (or in addit

106 relative contribution of DNA:RNA hybrids and misincorporated ribonucleotides to chromosome instabilit

107 cies for initiating and completing repair of misincorporated ribonucleotides, archaea such as Thermoc

108 rade transcription intermediates, and repair misincorporated ribonucleotides, in preventing genome in

109 iphosphate pools result in approximately two misincorporated rNMPs/kb of DNA.

110 incorporated template mutations, as well as misincorporated sequences in telomeres, a phenotype not

111 hts the exceptional ability of the enzyme to misincorporate T opposite C and T in sequence contexts c

112 We find that D246V misincorporates T opposite template bases G and C.

113 emplate C with extraordinarily low fidelity, misincorporating T, A, and C with unprecedented frequenc

114 Primase readily misincorporates the natural NTPs and will generate a wid

115 Herpes simplex virus-1 primase misincorporates the natural NTPs at frequencies of aroun

116 This enhanced preferential cleavage of misincorporated transcripts suggests an important role f

117 liate Paramecium tetraurelia stereotypically misincorporates TTP at telomerase RNA templating nucleot

118 y of a recoded beta-galactosidase variant by misincorporating tyrosine in place of phenylalanine.

119 wn about the role of base excision repair of misincorporated uracil in neuronal survival.

120 or PCNA-dependent postreplicative removal of misincorporated uracil.

121 the removal of adenines or 2-hydroxyadenines misincorporated with template guanines or 7,8-dihydro-8-