ライフサイエンスコーパス: mixer

1 orm mixing across streamlines that enter the mixer.

2 profiles at various points in the serpentine mixer.

3 l mixer, a 3D serpentine mixer, and a vortex mixer.

4 the 8 mus reaction time using a conventional mixer.

5 that is also present in the related protein Mixer.

6 om a thin plug of reagents introduced at the mixer.

7 the dead time (ca. 2 ms) of the stopped-flow mixer.

8 ltrarapid hydrodynamic focusing microfluidic mixer.

9 to the sample solution, assisted by a vortex mixer.

10 due 110 and the heme using a continuous flow mixer.

11 mance with experiments using microfabricated mixers.

12 oove, staggered herringbone, and herringbone mixers.

13 icantly different from that for conventional mixers.

14 triggered using an ultrafast continuous-flow mixer (150 micros dead time) reveal that heme attachment

15 time point attainable with a continuous-flow mixer (150 mus) confirm that the polypeptide ensemble is

16 achieved within the mixing dead time of the mixer (20 micros), and the first observable point for th

17 st reaction time probed using a conventional mixer (8 mus).

18 resentative microfluidic mixers: a Y channel mixer, a 3D serpentine mixer, and a vortex mixer.

19 rt the isolation of a zebrafish homologue of Mixer, a gene important for early endoderm formation in

20 asketweave" of channels, a chaotic advective mixer, a system with "braided" channels, and a 3D grid o

21 l data for three representative microfluidic mixers: a Y channel mixer, a 3D serpentine mixer, and a

22 electrospray potential; a low-volume static mixer; a column packed with silica-based reversed-phase

23 dominant inhibitory mutant demonstrate that Mixer activity is required for endoderm development.

24 ct alignment of the exit jet from the sample mixer allowed up to 2 ml of sample to be frozen in a thi

25 Without the presence of the mixer and at the same high flow rate, a channel length o

26 ercial NMR flow probe with a high-efficiency mixer and drive system.

27 In animal caps, VegT's ability to induce Mixer and Edd appears to depend upon SOX7 activity.

28 microfluidic device with integrated chaotic mixer and EMR has been successfully combined with subcut

29 itiating the reaction with a sub-millisecond mixer and following its progression by Trp fluorescence,

30 downstream of VegT by testing the ability of Mixer and Gata5 to rescue the expression of other endode

31 mn (PFC kit) was installed in between eluent mixer and injector to reduce contamination.

32 tion occurred normally and expression of bon/mixer and mezzo, zygotic transcription factors indicated

33 ain transcription factors of the Mix family, Mixer and Milk, but not Mix.1, mediate activin/TGF-beta-

34 temporal and spatial expression patterns of Mixer and Milk, lead us to propose a model for mesoendod

35 s, such as Fast-1 or the Mix family proteins Mixer and Milk, through a direct interaction between Sma

36 ify a short motif in the carboxyl termini of Mixer and Milk, which is demonstrated to be both necessa

37 expression in animal caps is independent of Mixer and Nodal signaling.

38 med using a simple confined impingement jets mixer and provides an efficient and reproducible method

39 we discuss the design and fabrication of the mixer and quantify the mixing efficiency using wide-fiel

40 cycle, we have used a rapid continuous flow mixer and resonance Raman spectroscopy to generate and i

41 We find that Nodal genes, Mixer and Sox17 have both shared and distinct sets of do

42 ike the known vertebrate endoderm regulators Mixer and Sox17, END-1 is a potent activator of endoderm

43 obtained with a microfabricated diffusional mixer and synchrotron radiation.

44 system have been found, i.e., volumes of the mixer and the coils, the rate of sample and reagent, pH

45 Using the ultra-fast microfluidic mixer and the freeze-quenching device, coupled with EPR,

46 etween the initiation of the reaction in the mixer and the observation be long enough that molecular

47 analyzing base flipping using a microfluidic mixer and two-color two-photon (2c2p) fluorescence lifet

48 family homeodomain proteins, such as Xenopus Mixer and zebrafish Bonnie and clyde (Bon), have been sh

49 g widely available three-syringe quench flow mixers and inexpensive reagents to study local changes i

50 compositional heterogeneity using high-shear mixers and membranes, to small-volume but more precise m

51 Compact and efficient wave mixers and optical sources spanning different frequency

52 he long mixing time of conventional solution mixers and the slow freezing time of cryogenic fluids.

53 c mixers: a Y channel mixer, a 3D serpentine mixer, and a vortex mixer.

54 casanova does not encode zebrafish Mixer, and mixer expression is normal in casanova mutant

55 d Xnr6, the homeodomain transcription factor Mixer, and the endodermal marker SOX17beta; both SOX7 an

56 urs within the 250 micros mixing time of the mixer, and there appears to be no further evolution of t

57 g resonant antennas, fixed RF amplifiers, RF mixers, and audio amplifiers.

58 lectronic devices such as Schottky diode THz mixers, and graphene modulators and photodetectors.

59 prototyping of three-dimensional "jumpers", mixers, and other key components of complex 3D microscal

60 ng columns, back-pressure regulators, static mixers, and packed-bed reactors.

61 g these effects in conjunction with those of Mixer- and Sox17beta-activating and dominant inhibitory

62 The transcription factors Fast-1 and Mixer are important terminal components of Smad2-mediate

63 cuit operates as a broadband radio-frequency mixer at frequencies up to 10 gigahertz.

64 igh denaturant in an ultrarapid microfluidic mixer at temperatures far below the thermal midpoint.

65 -plane F-S-F spin valves with a holmium spin mixer at the S-F interface providing evidence of a spin

66 pared favorably to both Tee and multilaminar mixers at low flow and was successfully used to screen a

67 hybridization chamber, which employs a fluid mixer based on chaotic advection theory to deliver targe

68 An economical and simple flow mixer based on magnetically driven agitation in a tube (

69 Judicious selection of mixers based on the chemistry requirement and real-time

70 n the approximately 200-microm-length static mixer bed, mixing was complete as determined by confocal

71 ivities, and determine whether FoxH1/Sur and Mixer/Bon can account for all Nodal signaling during emb

72 ese results and the association of FoxH1 and Mixer/Bon with phosphorylated Smad2 support a role for t

73 eins related to FoxH1 [Schmalspur (Sur)] and Mixer [Bonnie and clyde (Bon)] act within or downstream

74 Three mixer designs, a back-flow tee mixer (BT), an end-blocked membrane tee mixer (EMT), and

75 I) and the other with staggered herringbone mixers (called reactor II), were studied for the biolumi

76 Adding a mixer can circulate the input liquid, increasing the pro

77 the first time, that microfluidic diffusive mixers can be used for spatiotemporally high-throughput

78 particles to the surface, while the chaotic mixer circulates the particles to increase the number br

79 pumps controlled by micro stepper motors, a mixer connected to a serpentine tube, and a high-pressur

80 The architecture of the mixer consists of a simple "T" junction, made up by a 20

81 wave of expression, including Brachyury and Mixer, contribute to the regulation of genes expressed i

82 ne (DEMA) or triethylamine (TEA) through a T mixer coupled to a time-of-flight mass spectrometer.

83 In Mixer-depleted embryos, the expression of many signaling

84 Functional assays of Mixer-depleted vegetal cells showed that they have incre

85 hat dynamic hybridization based on a chaotic mixer design improves both the speed of hybridization an

86 The final mixer design was able to overcome stream splitting at hi

87 been developed with significant advances in mixer design, detection method and data analysis.

88 Using a microfluidic laminar flow mixer designed for studies of protein folding kinetics,

89 ng experiments of the original and optimized mixer designs show respective mixing times of 7 and 4 mu

90 Several mixer designs were fabricated and investigated.

91 Three mixer designs, a back-flow tee mixer (BT), an end-blocke

92 s cannot be prepared in current microfluidic mixer designs, nor is investigation of long-time scale r

93 1-3 microl/s) in comparison with other rapid mixer designs.

94 o minimize the mixing time of a microfluidic mixer developed for protein folding studies.

95 on instructions for both a microfluidic ring-mixer device and a 32-channel tabletop microfluidic cont

96 To validate the mixer device, the electrochemistry of ferrocene was also

97 New scaling relationships between mixer dimensions and operating conditions are derived an

98 Furthermore, we find that Mixer does not function primarily via Sox17 as previousl

99 to evaluate the performance of the microwave mixer, droplets with highly viscous fluid, 75% (w/w) gly

100 tee mixer (BT), an end-blocked membrane tee mixer (EMT), and a tubular membrane mixer (TM), were fab

101 sults indicate that employing a microballoon mixer enhances the detection sensitivity of the dengue v

102 gned and tested a novel microfluidic silicon mixer equipped with a new freeze-quenching device, with

103 At a sample flow rate of 20 microL/s, the mixer exhibits a short mixing deadtime of approximately

104 The mixer exploits hydrodynamic focusing to create a thin je

105 asanova does not encode zebrafish Mixer, and mixer expression is normal in casanova mutants, indicati

106 embrane protein Oep are essential for normal mixer expression.

107 scription factors belonging to the FoxH1 and Mixer families are required for facets of Nodal signalin

108 luidic device consists of microfluidic pumps/mixers, fluid channels, reagent chambers, and a DNA micr

109 ration of H(2)(18)O in a rapid-quenched-flow mixer followed by mass spectrometric quantitation.

110 ing a variable-time labeling pulse, a static mixer for HDX quenching, a proteolytic microreactor for

111 onstruction and characterization of a 100-pL mixer for liquids transported by electroosmotic flow.

112 ixer is the fastest reported continuous flow mixer for protein folding.

113 We have developed a microfluidic mixer for studying protein folding and other reactions w

114 nsive study has been done to optimize such a mixer for various applications.

115 lyses to illustrate the design principles of mixers for DEP-based concentrators.

116 Because mixers for DEP-based microconcentrators aim to circulate

117 xic buffers (using a computer-controlled gas mixer) for 45 min.

118 The flows are mixed in the mixer forming the emulsion in which the free fatty acids

119 identified endodermal determinants, require Mixer function.

120 maternal VegT, including Xsox17alpha, Bix4, Mixer, GATA4 and Edd.

121 el network functioning as an oxygen-nitrogen mixer generates differential oxygen concentration.

122 amagnetic nanoparticles, and several passive mixer geometries were assessed for their mixing efficien

123 al minimum of the mixing time by varying the mixer geometry and flow conditions.

124 A novel passive microfluidic silicon mixer has been designed, optimized and fabricated.

125 oom cold tap, one bath, and one bedroom sink mixer [hot/cold] taps).

126 hromatographic pump, with the worst of these mixers, imperfect mixing accounted for 99.6% of the obse

127 escribe the design and characterization of a mixer in which hydrodynamic focusing is decoupled from t

128 s for microvehicles, microfluidic valves and mixers in laboratory-on-chip devices, and adaptive optic

129 ction of Xbra and MyoD, but not Sox17beta or Mixer, in response to Xnr1.

130 Overexpression of mixer induces ectopic sox17-expressing cells in wild-typ

131 The mixer injects a narrow cylindrical stream (radius a < 1

132 rred in the 100-microm-wide, 300-microm-long mixer inlet channel where mixing would be achieved almos

133 The designed microwave mixer is a coplanar design with a small footprint, which

134 Together, these data suggest that Mixer is an embryonic transcription factor involved in s

135 A microfluidic mixer is applied to study the kinetics of calmodulin con

136 The performance of the mixer is characterized by mixing phenolphthalein solutio

137 Mixing in the microfluidic mixer is characterized, mechanisms underlying mixing are

138 A chip-based mixer is incorporated to transform the sample by dissolv

139 The optimized mixer is the fastest reported continuous flow mixer for

140 The mixer is used in conjunction with multiphoton microscopy

141 eam targets of VegT, the homeodomain protein Mixer, is expressed at high levels at the mesoderm/endod

142 Using the microfabricated mixer, Km and kcat values of 75 +/- 13 microM and 44 +/-

143 ich is populated within the dead time of the mixer (<10 mus) and has a characteristic Trp-59-heme dis

144 two species, design specifications for such mixers may be significantly different from that for conv

145 cruiting active Smad2/Smad4 complexes to the Mixer/Milk-binding site.

146 ichloromethane-methanol (8:2) and mixed in a mixer mill (MM400, Retsch technology).

147 by these receptors lead to the expression of mixer, Mixer then acts through casanova to promote the e

148 that quantitatively explains this biphasic 'mixer' model for cell size control.

149 We further explore mixer models of size control, where a timer phase preced

150 In the serpentine mixer, OCT image analysis revealed that the mixing effic

151 The alcove-based microfluidic silicon mixer offers unique advantages for its short deadtime an

152 e also tested the effect of the microballoon mixers on the detection of the dengue virus.

153 Although the mixer operates at low Reynolds numbers (less than 10(2))

154 Once familiar with quench-flow mixer operation and its calibration, nucleic acid labeli

155 We have developed a microfluidic mixer optimized for rapid measurements of protein foldin

156 RNA encoding a dominant negative version of Mixer or a morpholino against SOX17alpha2, both of which

157 re sources, fluid storage, mechanical pumps, mixers, or valves were necessary for fluid manipulation,

158 sion phenomena and a characterization of the mixer performance using microparticle image velocimetry,

159 Recent studies demonstrate that Fast-1 and Mixer play critical roles in the formation of endoderm a

160 This demonstrates that Mixer plays an essential role in controlling the amount

161 me is 2 orders of magnitude less than in any mixer previously coupled to a mass spectrometer.

162 nts include an adjustable position capillary mixer providing a variable-time labeling pulse, a static

163 In conjunction with a stopped-flow mixer, providing a time-resolved capability, this proced

164 ture dependent, increased temperature at the mixer resulted in higher sensitivity.

165 In the microfluidic silicon mixer, seven 10-microm-diameter vertical pillars are arr

166 xperimental use of the staggered herringbone mixer (SHM) for the signal enhancement of a microfluidic

167 examine the use of the staggered herringbone mixer (SHM) to increase the efficiency of analyte delive

168 we identify residues in the SIM critical for Mixer-Smad2 interaction and confirm their functional imp

169 y, we present a microwave-based microfluidic mixer that allows rapid mixing within individual droplet

170 We have constructed a laminar coaxial jet mixer that allows the measurement of UV-excited fluoresc

171 monolithically integrated optical waveguide mixer that can deliver multicolor light at a common wave

172 demonstrate a new droplet-based microfluidic mixer that induces chaotic mixing of crowded solutions i

173 We describe a microfluidic mixer that is well-suited for kinetic studies of macromo

174 complexes with transcription factors such as Mixer that only contain a SIM.

175 This paper presents a microfluidic mixer that uses acoustic stirring created by ultrasonic

176 idic mixing is usually achieved with passive mixers that are robust but poorly flexible, or active mi

177 framework for designing active microfluidic mixers that can efficiently homogenize a wide range of c

178 at are robust but poorly flexible, or active mixers that offer dynamic control but mainly rely on ele

179 used to isolate a homeobox-containing gene, Mixer, that can cause embryonic cells to form endoderm.

180 gated silver nanoparticles in a microfluidic mixer; the nanoparticle-reporter conjugates are then tra

181 e receptors lead to the expression of mixer, Mixer then acts through casanova to promote the expressi

182 rane tee mixer (EMT), and a tubular membrane mixer (TM), were fabricated and compared to three commer

183 have coupled a microfabricated laminar-flow mixer to a confocal optical system.

184 nes interdigitated electrodes with a chaotic mixer to achieve high-throughput (>100 microL/min) parti

185 ive magnetic separator combined with on-chip mixer to both purify and concentrate whole-particle huma

186 casanova acts downstream of, or parallel to, mixer to promote endoderm formation.

187 72 h, without the aid of a passive or active mixer to shorten the time of transport of a target molec

188 We then apply the mixer to single molecule FRET protein folding studies of

189 th a homemade continuous-flow rapid solution mixer to study the structural properties of the metastab

190 or ethanol) and widely available quench-flow mixers to reveal transient, often short-lived, intermedi

191 Mixer transcripts are found specifically in the prospect

192 The submillisecond mixer used here improves the time resolution by a factor

193 e discuss the design and optimization of the mixer using modeling of convective diffusion phenomena a

194 e submillisecond timescale with microfluidic mixers using a variety of probes including tryptophan fl

195 rescence versus distance downstream from the mixer, using an Hg(Xe) arc lamp for excitation and a dig

196 uidic device consists of microfluidic pumps, mixers, valves, fluid channels, reagent storage chambers

197 biochip device that consists of microfluidic mixers, valves, pumps, channels, chambers, heaters, and

198 ode (ILD) and a dielectric optical waveguide mixer via a gradient-index (GRIN) lens.

199 and compared to three commercially available mixers, Visco-Jet Micro mixer (VJM) and HS binary tee mi

200 mmercially available mixers, Visco-Jet Micro mixer (VJM) and HS binary tee mixer with 2 and 10 muL vo

201 The channel network composing the mixer was approximately 10 microm deep.

202 The microfluidic mixer was tested by the reaction between azide and myogl

203 A computer-controlled low-pressure mixer was used to generate solvent gradients.

204 Using this mixer we have measured sub-millisecond fluorescence quen

205 Using a microfluidic mixer we have observed the rate of intramolecular diffus

206 Mixing patterns in the vortex mixer were compared with light-microscopy and OCT image

207 d a low cost cavitation microstreaming based mixer which is easy to implement and use, but no compreh

208 isco-Jet Micro mixer (VJM) and HS binary tee mixer with 2 and 10 muL volume (HS-2 and HS-10) mixing c

209 yme, as a function of time in a microfluidic mixer with a dead time of approximately 20 mus.

210 microm, exits from the microfluidic silicon mixer with a linear flow velocity of 20 m/s.

211 em accommodated a miniature quartz capillary mixer with an approximately 0.5 microliter delivery volu

212 The microfluidic mixer with the simulation-optimized geometry was fabrica

213 tics within the continuous flow microfluidic mixer, with results showing good agreement with computat

214 design enables rapid in-lab construction of mixers without the need for expensive lithography-based

215 The mixer works essentially as a resonator that accumulates

216 Bix1, Bix3, Bix4, Milk (Bix2), Mix.1, Mix.2, Mixer, Xsox17 alpha, Gata4, Gata5, Gata6 and endodermin,

217 nscriptional regulators encoded by the genes Mixer, Xsox17, and HNF3beta; zebrafish One-eyed pinhead