ライフサイエンスコーパス: mobile phase

1 h 2% acetic acid/methanol (96:4, v/v) as the mobile phase.

2 ample treatment other than dilution with the mobile phase.

3 ium acetate water solution and methanol as a mobile phase.

4 dium (-)-dibenzoyl-l-tartarate as the chiral mobile phase.

5 e column to rid the ion-pair reagents of the mobile phase.

6 nd phosphate buffered saline solution as the mobile phase.

7 ationary phase and bile salt solution as the mobile phase.

8 series using a gradient of hexane-2-propanol mobile phase.

9 sensor and a PDZ domain (MAGI-1 PDZ1) in the mobile phase.

10 ttributed to the presence of methanol in the mobile phase.

11 d chromatography using an anionic surfactant mobile phase.

12 nts directly into the analyte solution or LC mobile phase.

13 y changing the amount of acetonitrile in the mobile phase.

14 t originally present in the sample or in the mobile phase.

15 e LC sample injection and delivery of the LC mobile phase.

16 and concentration of organic modifier in the mobile phase.

17 rometry (LC-MS) with methanol as the organic mobile phase.

18 n of hydrophobic albumin in a highly aqueous mobile phase.

19 data were acquired using acetonitrile as the mobile phase.

20 -bungarotoxin (alpha(x)beta(y) nAChR) to the mobile phase.

21 ups, and the ability to use a highly aqueous mobile phase.

22 onitrile in water with 0.1% formic acid as a mobile phase.

23 ation/immobilization (s/i) buffer and to the mobile phase.

24 ith pressure-driven flow used to deliver the mobile phase.

25 p APPI sensitivity when MeOH was used as the mobile phase.

26 atively, one might alter the chromatographic mobile phase.

27 cetonitrile/0.1% trifluoroacetic acid as the mobile phase.

28 dryness and the residue was reconstituted in mobile phase.

29 le (ACN): 0.1% phosphoric acid (60:10:30) as mobile phase.

30 C18 column with ACN-formic acid 0.1% as the mobile phase.

31 percritical fluid chromatography using a CO2 mobile phase.

32 red in-line ionic strength adjustment of the mobile phase.

33 tonitrile each containing formic acid as the mobile phase.

34 phy (UTLC) plates in 1-2 min using a ternary mobile phase.

35 etonitrile/1% CH3COOH (82/18) as the loading mobile phase.

36 egrees C and zero magnesium concentration in mobile phases.

37 lectivity, using both organic and water-rich mobile phases.

38 en added (generally, 0.1%), were employed as mobile phases.

39 rly observed for peptides when using high-pH mobile phases.

40 retention of solutes with high water-content mobile phases.

41 aqueous ammonium formate and acetonitrile as mobile phases.

42 lammable solvents (e.g., hexane) are used as mobile phases.

43 e positive mode of ionization with common LC mobile phases.

44 l (compared to 2(3+)) when mixed with acidic mobile phases.

45 ary phases and step-gradient aqueous-ethanol mobile phases.

46 s to separate intact proteins using volatile mobile phases.

47 of mixed-solvent liquid chromatography (LC) mobile phases.

48 h a mixture of acetonitrile and water as the mobile phase (15/85, v/v).

49 ted with a series of buffered methanol-water mobile phases (20/80, v/v).

50 C) are demonstrated with typical low density mobile phases (94% CO2).

51 Mobile phase A was 20mmolL(-1) ethylenediaminetetraaceti

52 anol, 14.5mM triethylamine, and 5% methanol (mobile phase A) and 385mM hexafluoro-2-propanol, 14.5mM

53 tion into plasma allowed use of organic-rich mobile phase, achieving species separation in 4 min.

54 city of the sample matrix and sensitivity to mobile phase acidity, are identified and resolved.

55 by using the alternative means of expressing mobile phase acidity.

56 When warfarin was used as a mobile-phase additive in competition studies with tamoxi

57 Competitive studies using tamoxifen as a mobile-phase additive indicated that tamoxifen had a neg

58 In this study, different mobile phase additives were tested in order to improve t

59 ce of polar mobile-phase solutions or acidic mobile-phase additives (e.g., formic or trifluoroacetic

60 ILIC/MS method was optimized on the basis of mobile-phase additives and pH, followed by evaluation of

61 polar species, a series of low-concentration mobile-phase additives were used (NaCl, LiBr, NH4OH).

62 The mobile phase affects the ionization state of analytes an

63 Ammonium fluoride, added to the mobile phase, aids in the formation of pseudomolecular o

64 ss distribution of the compound between the "mobile phase" (air and dust particles settled on the car

65 mogeneous stationary phase and a homogeneous mobile phase (although there may be two or several types

66 hyl acetate/formic acid (6:10:1, v/v) as the mobile phase and 1% vanillin hydrochloric solution as st

67 size exclusion chromatography (SEC) such as mobile phase and column interaction effects on protein s

68 arise due to the compressible nature of the mobile phase and detector idiosyncrasies to eliminate ba

69 selected proteins were eluted with an acidic mobile phase and identified in two ways.

70 M, determination rate, ca. 10 samplesh(-1), mobile phase and ISA solution consumption, ca. 2.6 mL pe

71 d 1.65 mL/min, with pure acetonitrile as the mobile phase and naphtho[2,3-a]pyrene as the retained co

72 radient elution in acidulated water-methanol mobile phase and octadecyl-silica columns.

73 pH units, depending on the initial pH of the mobile phase and the applied current.

74 The gradient composition of the mobile phase and the flow rate were optimized with respe

75 rstanding of the processes that occur in the mobile phase and the stationary phase.

76 compounds in a variety of acetonitrile/water mobile phases and at different temperatures.

77 has been made transferable between different mobile phases and instrument setups by using a suitable

78 the use of solvents other than those of the mobile phase, and (iii) the need to stop the mobile phas

79 the missing cofactor is added to the column mobile phase, and the enzyme converts substrate into pro

80 ffect of matrices, concentrations of aqueous mobile phase, and types of LC modifiers.

81 heir increasing of retention at organic-rich mobile phases (approximately >90% v/v for acetonitrile w

82 on of chromatographic columns containing two mobile phases are presented.

83 hy using methanesulfonic acid (75 mM) as the mobile phase at 0.25 mL/min flow rate.

84 s particularly when using low ionic strength mobile phases at low pH (e.g., formic acid), even with h

85 nol, 14.5mM triethylamine, and 90% methanol (mobile phase B).

86 (NH4)2CO3 in 1% v/v methanol (pH 9.0) formed mobile phase B.

87 tion gradient with acetonitrile and water as mobile phases (both with formic acid at 0.1%).

88 particle: acetonitrile in the interparticle mobile phase, C(18)-chain associated acetonitrile, and a

89 aining peptide eluted out of LC in an acidic mobile phase can be rapidly reduced prior to MS analysis

90 The pH of the mobile phase can govern the detection selectivity, since

91 on behavior of bases (cationic acids) in the mobile phases can be better predicted by using the pH(ab

92 articles settled on the carpet) and the "non-mobile phase" (carpet fibers and pad) and the removal ra

93 peration to retain detector sensitivity when mobile phase changes.

94 Of the 132 column and mobile phase combinations examined for each mixture, a s

95 ing NBD-F tagged amino acid enantiomers with mobile phases compatible with MS detection.

96 ut fluids that are commonly used as the main mobile-phase component in SFC, such as CO2, are relative

97 th peak distortions due to strongly retained mobile phase components in other modes of liquid chromat

98 Mobile phase components needed to be sufficiently volati

99 The influence of mobile-phase components on chromatographic performance a

100 The use of a mobile phase composed by ammonium formate-methanol in a

101 several catecholamines were resolved with a mobile phase composed of 0.05M phosphate buffer (pH 5.5)

102 re collected on to a C18 guard column with a mobile phase composed of 90% D2O/10% acetonitrile-D3 and

103 lysis employed silica gel-coated TLC plates, mobile phase composed of chloroform:methanol:water:25% a

104 re silica stationary phase with an isocratic mobile phase composed of CO2/methanol solvent with addit

105 the elution of compounds in a higher organic mobile phase composition (retention times were approxima

106 degrees C), flow rate (1.0-2.5 mL min(-1)), mobile phase composition and equilibration time (1-5 min

107 ssed as are the effects of mat thickness and mobile phase composition on the chromatographic properti

108 The mobile phase composition was optimized to maintain the p

109 < linear < ccc, is constant upon changes of mobile phase composition, gradient slope, and plasmid si

110 ntion factors at extremes of temperature and mobile phase composition.

111 the complex mixture is affected by both the mobile-phase composition and the pressure, and the resol

112 ic conditions includes the column selection, mobile-phase composition, pH value, buffer type, and con

113 ork, different CEC-ESI-MS parameters such as mobile-phase composition, sheath liquid, and spray chamb

114 ionization efficiency of peptides under each mobile-phase composition.

115 A linear velocity surge caused by mobile-phase compression was investigated as a source of

116 aration was done with isocratic elution of a mobile phase comprising water (with 0.5% formic acid) an

117 was accomplished with isocratic elution of a mobile phase comprising water and methanol (92:8 v/v) on

118 eversed phase HPLC-ICP-MS, such as pH of the mobile phase, concentration of ion pairing reagents, typ

119 Both isocratic and gradient mobile phase conditions were used.

120 raphic analysis allowed the use of isocratic mobile-phase conditions to achieve effective and efficie

121 aphic columns when used under highly aqueous mobile-phase conditions.

122 n Hibar C18 column (250x4.6mm, 5micro) using mobile phase consist of acetonitrile: water (90:10, v/v)

123 The mobile phase consisted of 0.2 M phosphate buffer (pH 7.0

124 tion on silica gel-coated HPTLC plates using mobile phase consisted of chloroform:methanol:acetone:25

125 The mobile phase consisted of water and acetonitrile, with a

126 -crystalline bilayer phase, and an extremely mobile phase consistent with small vesicles or micelles.

127 c separation was achieved on C8 column using mobile phase consisting of (A) methanol:acetonitrile (8:

128 mm, 5 mum) column by gradient elution with a mobile phase consisting of 0.1% trifluoroacetic acid (pH

129 ne in food supplements was performed using a mobile phase consisting of 100% methanol, column tempera

130 ned on a Chiralcel(R) OJ-RH column using the mobile phase consisting of 10mM aqueous ammonium acetate

131 hic separation under gradient elution with a mobile phase consisting of acetonitrile and trifluoroace

132 enyl column (50 x 2.0 mm) and supported by a mobile phase consisting of acetonitrile plus 0.1% formic

133 mm, 5 mum) column by gradient elution with a mobile phase consisting of ammonium acetate 0.05 M and a

134 An optimized mobile phase consisting of methanol and 25 mM triethylam

135 With the use of a mobile phase consisting of slightly higher acetonitrile

136 achieved using a C18 column with a gradient mobile phase consisting of solvents A (0.1% formic acid

137 thylene bridged hybrid phenyl column using a mobile phase consisting of water and methanol containing

138 ographic conditions were found to comprise a mobile phase containing 60% methanol, 40% 50 mM pH 4.1 a

139 The optimized CEC-ESI-MS conditions were mobile phase containing 90/10 ACN/2.5 mM Tris, pH 8, she

140 olar organic mode (e.g., methanol or ethanol mobile phase containing a barium salt additive).

141 alyzed by reversed-phase HPLC method using a mobile phase containing acetonitrile:methanol:2-propanol

142 bolites was carried out isocratically with a mobile phase containing both positively and negatively c

143 The mobile phase containing CLA isomers eluting from the Ag(

144 sing isocratic elution with a methanol/water mobile phase containing tetrabutylammonium fluoride (Bu4

145 lumn, acetonitrile-trifluoroacetic acid as a mobile phase, coupled with UV detector at 205 nm, was su

146 tes and matrix constituents solubility after mobile phase decompression.

147 n the retention factor (K) and the pH of the mobile phase demonstrate that the binding of cis-diols t

148 the dependency of solute retention with the mobile-phase density, complicating linear extrapolation

149 ution with mixtures of methanol and water as mobile phases; detection wavelength was set at 240 nm fo

150 Dopant was not necessary for hexane-based mobile phases due to their self-doping effects.

151 tered when moving from an aqueous to organic mobile phase during a gradient elution, a key analytical

152 mobile phase, and (iii) the need to stop the mobile phase flow at any time during the full analytical

153 0.1% formic acid (50:50, v/v) and an optimal mobile phase flow rate of 0.2 mL/min.

154 NPs exhibited a retention time of 771 s at a mobile phase flow rate of 1 mL min(-1).

155 h direct injection onto the PLOT column at a mobile phase flow rate of 20 nL/min.

156 ides excellent chromatographic resolution at mobile phase flow rates from 1 to 55 muL min(-1).

157 is obtained when the plate is soaked in the mobile phase for a defined time before each run.

158 ns for the generation of Fe nanoparticles, a mobile phase for As adsorption currently not a part of r

159 An anionic micellar medium was added to the mobile phase for increasing the fluorescence intensity a

160 methanol/aqueous trifluoroacetic acid as the mobile phase for size exclusion chromatography-ESI-MS an

161 se low vapor pressure ionic liquids as their mobile phases for sensing atmospheric analytes.

162 nt column temperature, stationary phase, and mobile phase gradient conditions.

163 imized for organic modifier, ionic modifier, mobile phase gradient, flow rate, column type, MS source

164 ction with chromatographic separations using mobile phase gradients.

165 reagent, m-nitrobenzyl alcohol (m-NBA), into mobile phases greatly facilitates the analysis of acidic

166 purpose, the addition of thiosulfate to the mobile phase has been used to elute Ag(I) species from t

167 ) values for a number of common LC and LC-MS mobile phases have been determined.

168 in the DLLME with the reduced consumption of mobile phase in capillary HPLC.

169 out using water and ammonium bicarbonate as mobile phase in gradient mode.

170 ition of these supercharging reagents to the mobile phase in liquid chromatography (LC)-MS/MS increas

171 he low viscosity and high diffusivity of the mobile phase in supercritical fluid chromatography (SFC)

172 e change in the refractive index (RI) of the mobile phase in very fast gradients causes extremely ser

173 intraluminal organization with two phases: a mobile phase in which secretory proteins diffuse as in t

174 imation of the volumes of the stationary and mobile phases in dynamic equilibrium with eluents of var

175 The key feature is a mobile phase, in which hydrophilic and hydrophobic compo

176 At organic-rich mobile phases, in fact, stationary phases are characteri

177 ither post-column or by incorporation in the mobile phase increases specificity for all of the MC whi

178 ion following an increase of acetonitrile in mobile phase) initially exhibited by perfluoroalkyl acid

179 either the mobile phase or at the stationary/mobile-phase interface.

180 tation pattern, without any matrix effect or mobile-phase interference.

181 ndent accumulation of pyrene from an aqueous mobile phase into the center of individual C18-chromatog

182 ding behavior; overloading is reduced as the mobile-phase ionic strength is increased.

183 The mobile phase is driven by electroosmotic flow, while the

184 d from potential contamination); (IV) the LC mobile phase is of much lower viscosity with respect to

185 y obtained with ammonium tartrate in the HIC mobile phases is orthogonal to that of reverse phase chr

186 luate and correct for the impact of stagnant mobile phase mass transfer.

187 dge RP-18e (Merck), 10x4.6mm, with a washing mobile phase (methanol:water, 92:8, (v/v)) at a flow rat

188 The aqueous mobile phase migrates only through the channel due to th

189 Further, they were used in all mobile phase modes and with high flow rates and pressure

190 nsitivity is dramatically enhanced by use of mobile phase modifiers (i.e., ammonium formate or sodium

191 polarities amenable to pSFC with appropriate mobile-phase modifiers and additives under normal-phase

192 phenyl-2-oxoethanal as the negative-ion-mode mobile-phase modifiers for the analysis of peptides.

193 PCI and much more sensitive than ESI without mobile-phase modifiers.

194 alcohol to the water (W)-acetonitrile (ACN) mobile phase (MP).

195 on a Synergi Hydro analytical column with a mobile phase of 0.02 M ammonium formate in water and ace

196 e quinolones were resolved in <22min using a mobile phase of 0.05M SDS - 7.5% 1-propanol - 0.5% triet

197 ones were eluted without interferences using mobile phase of 0.05M SDS/12.5% 1-propanol/0.5% triethyl

198 uorescence detection within 10min by using a mobile phase of acetonitrile and acetate buffer of pH 6.

199 as achieved using a gradient elution, with a mobile phase of acetonitrile, formic acid, and ammonium

200 ected to high-performance LC with an optimal mobile phase of acetonitrile-water containing 0.1% formi

201 used under the optimum conditions predicted: mobile phase of H2SO4 0.005 mol L(-1) solution, flow rat

202 Upon addition of 0.2% TFE to the mobile phase of nLC/MS experiments, tryptic peptide iden

203 Using a mobile phase of water:acetonitrile:methanol (83:6:11) at

204 th a C18 column (2.1 x 100 mm, 1.7 mum) with mobile phases of 0.1% formic acid in water and acetonitr

205 different stationary phases in contact with mobile phases of various water/methanol ratios.

206 and the final methanol concentration in the mobile phase on the peak resolution and peak symmetry wa

207 sm is dominated by ion-pairing in either the mobile phase or at the stationary/mobile-phase interface

208 and ensured few changes were required in the mobile phase or other parameters.

209 Different mobile phase organic solvents and ion-pairing reagents w

210 ditions and subsequent stability analysis in mobile phase, PBS buffer, and rat serum of 12 aryl sulfo

211 mobile phase velocities, the friction of the mobile phase percolating through the column bed generate

212 Mobile phase pH is very important in LC, but its correct

213 reagent (IPR) concentration, counterion, and mobile phase pH on the quality of the RPIP-UPLC separati

214 We have found that a mobile phase pH value near the pI of the zwitterionic ad

215 on (<9.9%CV), recovery (83-99%), robustness (mobile phase pH, column temperature and flow rate) and s

216 hromatographic parameters (stationary phase, mobile phase pH, temperature, organic solvent, and gradi

217 s used to relate analyte retention time with mobile-phase pH and organic modifier content.

218 of test set proteins across a wide range of mobile-phase pH conditions.

219 cetonitrile content, salt concentration, and mobile-phase pH with R(2) > 0.95.

220 rious modes of protein chromatography at any mobile-phase pH, which may have significant implications

221 ed under all LC-MS conditions, regardless of mobile-phase pH.

222 combinations of organic modifier content and mobile-phase pH.

223 phase column with gradient elution of basic mobile phases (pH 9.2).

224 r peak shapes, particularly when eluted with mobile phases preferred for electrospray ionization mass

225 er, the water/acetonitrile and water/acetone mobile phases produced the better chromatographic separa

226 he implementation of chromatography with two mobile phases produces a chromatographic "window".

227 In this way, mobile phase protons are prevented from interfering with

228 ncrease of organic modifier, over the entire mobile phase range.

229 bicarbonate and pyridine buffer solutions as mobile phases, respectively.

230 ively high percentage of acetonitrile in the mobile phase, resulting in stable and high efficiency io

231 When mobile-phase salt content is increased, cationic analyte

232 ng the retention of cationic analytes as the mobile-phase salt content is varied.

233 stry and concentration, resin chemistry, and mobile-phase salt counterion on the efficacy and selecti

234 of amphiphilic (surfactant) molecules to the mobile-phase solution in order to bring about the retent

235 nary phase particles with acetonitrile-water mobile phase solutions by confocal Raman microscopy.

236 bilization, because of the presence of polar mobile-phase solutions or acidic mobile-phase additives

237 Herein, we optimize the choice of mobile phase solvent in a gradient program with three pa

238 alcohols on the particle surface and in the mobile phase solvent.

239 dopants are required depends on the IP(s) of mobile-phase solvent(s) and solvent complexes, and photo

240 With the use of water-glycerol mobile phase spanning a wide range of viscosities, the o

241 rabutylammonium for carboxylic acids) in the mobile phase suppresses the ESI-MS signals in the gas ph

242 uted using a gradient methanol/water/toluene mobile phase system at a flow rate of 0.5 mL min(-1).

243 The gradient mobile phase system consisted of 385mM hexafluoro-2-prop

244 The overall performance of this mobile phase system was found comparable to ammonium ace

245 CX mixing ratio as the ionic strength of the mobile phase system.

246 ationary phases in conjunction with multiple mobile-phase systems, as applied to the separation of 45

247 ous attempt, the vaporization of solutes and mobile phase takes place at atmospheric pressure into a

248 The column, mobile phase, temperature and flow rate were optimised t

249 e successive physical transformations of the mobile phase that take place in very high pressure liqui

250 in their ability to resolve glycoforms using mobile phases that are compatible with online liquid chr

251 ntial-oil "matrix", replacing it with the LC mobile phase (the GC system is more protected from poten

252 Because water was the mobile phase, the retention factor could not be kept con

253 Indeed it was found that, at organic-rich mobile phases, the transfer from the mobile to the stati

254 olumn is eluted with a predominantly organic mobile phase, then solutes can be retained through hydro

255 such as postcolumn organic enrichment of the mobile phase to enhance ESI efficiency.

256 cellar conditions using 1-2% (v/v) 1-butanol mobile phase to remove plasma proteins and concentrate t

257 r for the transfer of polar species from the mobile phase to residual silanols, and this reduced barr

258 oacetic acid as additive in a CO(2)/methanol mobile phase to suppress deprotonation of peptide carbox

259 ring effects by using (1) low ionic strength mobile phases to reduce electrostatic screening, (2) a b

260 nd to develop gel porosity in contact with a mobile phase ultimately affecting the chromatographic pe

261 aqueous acetic acid/methanol mixture as the mobile phase under gradient conditions.

262 Moreover, the mobile phase used allows high quality on-line MS detecti

263 e linear velocity and the composition of the mobile phase used in the second dimension, its initial o

264 te in 22%v/v methanol solution (pH 4) as the mobile phase using isocratic elution.

265 xtures of small molecules are carried out at mobile phase velocities close to (for isocratic runs) or

266 ith very fine particles are operated at high mobile phase velocities, the friction of the mobile phas

267 axes are linear: column length vertical and mobile phase velocity horizontal.

268 ate height with an increase of around 15% in mobile phase velocity in nonretained measurements of Cou

269 al plates as a function of column length and mobile phase velocity is a surface (z direction) to the

270 HETP tend to increase faster with increasing mobile phase velocity than the calculated values.

271 affected in resolving power by increases in mobile-phase velocity than the sub-2 microm porous silic

272 d due to the influence of temperature on the mobile phase viscosity and on the equilibrium constant o

273 column, the temperature dependencies of the mobile phase viscosity, and the retention factor of the

274 urea, did provide an accurate measure of the mobile-phase volume but only over a limited range of elu

275 a viable measure of the kinetic void volume (mobile-phase volume) of the column.

276 The mobile phase was a mixture of methanol and water at vari

277 At 2 min, mobile phase was changed to elute and separate PET radio

278 The relatively simple mobile phase was compatible with mass spectrometric dete

279 neous heat transfer from the assembly to the mobile phase was obtained.

280 The use of nonafluoropentanoic acid in mobile phase was omitted, SPE recoveries of 82+/-3% and

281 obtained with a gradient method in which the mobile phase was water (0.1% acetic acid) as solvent A a

282 ality grades, postcolumn modification of the mobile phase) was investigated in a pragmatic and decisi

283 reas the other set received tap water as the mobile phase water.

284 al silanols in contact with a water/methanol mobile phase, we show that the molecular-level retention

285 tion, where the chemical constituents of the mobile phase were modified stepwise during analysis, thi

286 The aqueous and organic mobile phases were 0.1% formic acid in water and acetoni

287 The most superior mobile phases were also applied on instant thin-layer ch

288 For radio-TLC quality control, various mobile phases were analyzed using silica gel 60 plates a

289 The residence times of the two mobile phases were determined by tracer pulse chromatogr

290 Several liquid chromatography stationary and mobile phases were evaluated, and it was found that hydr

291 The (W)(S)pHs of the mobile phases were successively adjusted with addition o

292 h of analytical column, and flow rate of the mobile phase, were optimised for five selenium species;

293 ver, dopants became unnecessary for the MeOH mobile phase when the Ar lamp was used.

294 One phase is used as the mobile phase when the other, the stationary phase, is he

295 ies by anion exchange chromatography using a mobile phase which is a 1:10 dilution of the extracting

296 rming CEC/MS utilizing a harsh polar organic mobile phase, which was not previously successful using

297 LC-based column method using a fully aqueous mobile phase with 5 mM CaCl(2) at pH 4.5.

298 general, androgens required a stronger UPLC mobile phase with a slower flow rate and ESI of the oppo

299 rol was performed on silica gel 60 plates, 4 mobile phases with suitable separation properties and co

300 ime direct comparison of acidities of any LC mobile phases, with different organic additives, differe