ライフサイエンスコーパス: molybdate

1 e presence of MoeA and low concentrations of molybdate.

2 lation of a blue compound when supplied with molybdate.

3 co in the presence of high concentrations of molybdate.

4 ecursor Z, purified MPT synthase, and sodium molybdate.

5 co) synthesized de novo from precursor Z and molybdate.

6 ar translocation of the AhR, is inhibited by molybdate.

7 perator DNA was 6 times lower than with ModE-molybdate.

8 E(T125I) and ModE(Q216*), were unaffected by molybdate.

9 egion of modA operator DNA in the absence of molybdate.

10 the K(d) increased to 8 nM in the absence of molybdate.

11 n the presence of molybdate and 4 nM without molybdate.

12 ompetitively inhibited growth stimulation by molybdate.

13 plant hsp90 are stabilized by p23 and not by molybdate.

14 of mog, by culture in high concentrations of molybdate.

15 -plant hsp90 complexes are not stabilized by molybdate.

16 odE, which acts as a repressor when bound to molybdate.

17 led to a transformation of thiomolybdates to molybdate.

18 f the nutrient phosphorus, which reacts with molybdate.

19 .0 +/- 0.2 pM; 50 000-fold more tightly than molybdate.

20 ined from oxidized crystals soaked in sodium molybdate.

21 activity in the absence of externally added molybdate.

22 nity manner with and without the addition of molybdate.

23 modA and modBC was induced by starvation for molybdate.

24 sms on CaAl LDHs with increasing loadings of molybdate.

25 dc37 in salt-stable complexes independent of molybdate.

26 p90 and Cdc37 independent of the presence of molybdate.

27 t had no negative effect on higher thiolated molybdates.

28 A high concentration of molybdate (10 microM) supported normal growth of the mod

29 90 (elevated temperature, ATP, Nonidet P-40, molybdate), a five-membered (p23.hsp90.p60.hsp70.hsp40)

30 Tungstate interference of molybdate acquisition by the cell is apparently due in p

31 MoO4), ferrimolybdite (Fe2(MoO4)3.8H2O), and molybdate adsorbed on ferrihydrite (Fe(OH)3 - MoO4).

32 It was found that ferrimolybdite and molybdate adsorbed on ferrihydrite initially dissolves i

33 n the TMF are powellite, ferrimolybdite, and molybdate adsorbed on ferrihydrite.

34 Molybdate also reduced the amount of free hsp90 present

35 and ModE(T125I) was 3 nM in the presence of molybdate and 4 nM without molybdate.

36 Complex formation is strongly promoted by molybdate and by the nonionic detergent Nonidet P-40.

37 on proteins, we characterized the effects of molybdate and geldanamycin on hsp90 function and structu

38 We used molybdate and monofluorophosphate as inhibitors of sulfa

39 ectrophoresis protocol to examine binding of molybdate and other anions to the ModA periplasmic prote

40 sphate precipitation reaction using ammonium molybdate and triethylamine under low pH has been applie

41 ile Cj0303 is unable to discriminate between molybdate and tungstate (K(D) values for both ligands of

42 Molybdate and tungstate are strong inhibitors of the pur

43 Thus, molybdate and tungstate bridge the FeZn active site like

44 FS regions shows that the iron sites of both molybdate and tungstate complexes are best simulated by

45 The asymmetric bidentate bridging mode of molybdate and tungstate helps explain the effect of thes

46 y revealed apparent Kd values of binding for molybdate and tungstate of 3 and 7 microM, respectively.

47 te-reducing bacteria, high concentrations of molybdate and tungstate oxyanions inhibit growth, thus r

48 Whereas molybdate and tungstate were bound with high affinity (a

49 it can be inhibited by low concentrations of molybdate and vanadate.

50 CSP of NaCl, alkali molybdates and V2 O5 with small concentrations of water

51 bohydrate premetabolism, showing how borate, molybdate, and calcium minerals guide the formation of t

52 The enzyme was inhibited by vanadate, molybdate, and EDTA but was resistant to inorganic phosp

53 , Brassica spp. seedlings were supplied with molybdate, and the effects on plant physiology, morpholo

54 e early transition metal oxoanions vanadate, molybdate, and tungstate are widely used inhibitors for

55 Neither tungstate nor vanadate replaced molybdate, and tungstate competitively inhibited growth

56 ntaining various concentrations of selenite, molybdate, and various purine substrates.

57 trong pre-edge absorption from the distorted molybdate anions leaves the oxidation state ambiguous be

58 s; in the absence of steroids, either p23 or molybdate are also required as reported previously.

59 oxoanion analogues orthovanadate, tungstate, molybdate, arsenate, and sulfate were shown to inhibit t

60 ModE-molybdate at 5 nM completely protected the modABCD opera

61 a conformational change of the protein upon molybdate binding.

62 nsive conformational changes not only in the molybdate-binding domain, but also in the DNA-binding do

63 part of the fused gene lacked a recognizable molybdate-binding domain.

64 The X-ray structures of the cytoplasmic molybdate-binding protein ModG from Azotobacter vineland

65 The C-terminal domain contains the molybdate-binding site and residues implicated in bindin

66 E dimer allows the probable locations of the molybdate-binding sites of the latter to be assigned.

67 n to the oxidized form, the structure of the molybdate-bound form of the protein was determined from

68 should improve DNA binding of the activated molybdate-bound ModE.

69 ons, the nifH1 mutant grew in the absence of molybdate but not in its presence, using VnfH, while the

70 hosphatases (sodium orthovanadate and sodium molybdate) but not by inhibitors of serine/threonine pho

71 ctably transport low concentrations of 99Mo (molybdate), but did transport high concentrations.

72 es molybdenum incorporation at low levels of molybdate, but MogA has an alternative function, possibl

73 The ABC transporter, MolBC-A, imports molybdate by passing substrate from the binding protein

74 amino acids is essential for the binding of molybdate by the ModE protein.

75 We show how this fate might be avoided using molybdate-catalyzed rearrangement of a branched pentose

76 the Hsp90 inhibitory agents geldanamycin and molybdate, Cdk2 is shown to be a genuine client of the H

77 The conversion of MPT into Moco by molybdate chelation apparently occurs concomitantly with

78 s or with the tyrosine phosphatase inhibitor molybdate completely blocked stimulation of HCO(3)(-) ab

79 ly mimic in its conformation the native ModE-molybdate complex, which binds to a DNA sequence motif o

80 ahedral geometry was observed for the weaker molybdate complex.

81 om Escherichia coli functions as a sensor of molybdate concentration and a regulator for transcriptio

82 In periods of high extracellular molybdate concentration, H. influenzae makes use of para

83 ene product in response to the intracellular molybdate concentration.

84 This phenotype was rescued by increasing molybdate concentrations in the nutritive solution, or u

85 experiments with pure cytochrome showed that molybdate could oxidize the reduced cytochrome, although

86 H1, which was also made in cells starved for molybdate, could substitute for VnfH.

87 tely 80%) in settling particles amended with molybdate, demonstrating the prominent role of biologica

88 ansporter, is repressed by ModE in vivo in a molybdate-dependent fashion.

89 sm by binding to specific DNA sequences in a molybdate-dependent fashion.

90 ption factor to help coordinate nitrate- and molybdate-dependent napF expression by the Fnr, NarP, Na

91 s, we confirmed that IHF plays a role in the molybdate-dependent regulation of dmsA-lacZ expression i

92 The molybdate-dependent transcriptional regulator (ModE) fro

93 Both p23 and molybdate enhance and stabilize the nucleotide-bound sta

94 h the reaction of Si with acidified ammonium molybdate, followed by precipitation with triethylamine

95 Borate, sulfate, phosphate, and molybdate had essentially no effect on Apo activation an

96 Molybdate has the same ability as p23 to stabilize GR he

97 anscriptional factors controls tungstate and molybdate homeostasis in sulfate-reducing deltaproteobac

98 ires an ATP-regenerating system, dithionite, molybdate, homocitrate, and at least NifB-co (the metabo

99 We also establish that only molybdate, homocitrate, MgATP, and Fe protein are essent

100 se genes, the vupABC genes were repressed by molybdate; however, unlike the V-nitrogenase genes the v

101 High-affinity transport of molybdate in A. variabilis was mediated by an ABC-type t

102 controlling the homeostasis of tungstate and molybdate in sulfate-reducing deltaproteobacteria.

103 he formation of a complex of As(V) ions with molybdate in the presence of 50.0 mmol L(-1) sulfuric ac

104 e activation/reduction pathway combines with molybdate in the production of activated molybdenum.

105 e hydrogen sulfide is known to interact with molybdate in the production of thiomolybdate, it is poss

106 After the inclusion of sodium molybdate in the reconstitution mixture, active sulfite

107 us oxyanions tested, only tungstate replaced molybdate in the repression of modA by ModE, but the aff

108 tion constant (Kd) of 45 nM, and addition of molybdate, in physiologically relevant amounts, signific

109 to the moaA promoter appeared to be largely molybdate independent both in vitro and in vivo.

110 The molybdate-independent mutant ModE proteins apparently mi

111 When supplied with (colorless) molybdate Indian mustard (Brassica juncea) seedlings acc

112 t-accumulated Mo was different than that for molybdate, indicating complexation with a plant molecule

113 cin prevented hsp90 from assuming natural or molybdate-induced conformations that allow salt-stable i

114 While molybdate inhibited As biomethylation, this effect was u

115 Molybdate inhibited hsp90-mediated p56lck biogenesis and

116 showing significant sulfate stimulation and molybdate inhibition of methylation in SR sediments.

117 In this study, we demonstrate that molybdate inhibits AhR gene activation in both HepG2 and

118 hich MtMOT1.3 is responsible for introducing molybdate into nodule cells, which is later used to synt

119 otoxicity experiments on MoS2 nanosheets and molybdate ion controls reveal the relative roles of the

120 The location of the molybdate ion near heme IV in the crystal structure sugg

121 of the reaction between orthophosphates and molybdates ions where ascorbic acid and antimony potassi

122 as is nitrate reductase; thus, transport of molybdate is important for growth of this strain.

123 also a Type II importer, but its substrate, molybdate, is approximately 10-fold smaller than vitamin

124 ression when the essential enzyme component, molybdate, is limiting in the cell environment.

125 nct effect on the conformation of Hsp90, and molybdate lowered the concentration of novobiocin requir

126 as luminescence, of rare earth tungstate and molybdate materials.

127 n in a defined culture medium, but only when molybdate (Mo) and selenite (Se) were also added.

128 303 and Cj1538-1540) homologous to bacterial molybdate (ModABC) uptake systems and the tungstate tran

129 the larger membrane-spanning subunits of the molybdate (ModBC) and maltose (MalFGK) ABC transporters.

130 a trimer capable of binding a total of eight molybdate molecules that are distributed between two dis

131 seawater and sediment should be observed if molybdate (MoO4(2-)) is quantitatively transformed to te

132 nts and X-ray techniques was used to examine molybdate (MoO4(2-)) sorption mechanisms on CaAl LDHs wi

133 The use of rare earth tungstate and molybdate nano- and micromaterials as single materials f

134 three distinct regions of protection by ModE-molybdate on the modA operator/promoter DNA, GTTATATT (-

135 fects of sulphate-mimetics (like chromate or molybdate) on sulphate transport.

136 s ATP-induced state is achieved by including molybdate or by use of the ATP analogue ATPgammaS.

137 wo cultures, Hg methylation assays following molybdate or chloroform inhibition (a specific inhibitor

138 When molybdate or p23 is also present during the incubation w

139 r dichroism spectroscopy indicated that when molybdate or tungstate bind to ModE there is little chan

140 Addition of molybdate or tungstate to the protein results in almost

141 were expressed in the presence or absence of molybdate or vanadate.

142 id not grow diazotrophically with or without molybdate or vanadate.

143 pendent process that is stimulated by sodium molybdate, other group VI-A transition metal oxyanions,

144 t 2.22 A, the latter being associated with a molybdate oxygen.

145 demonstrated that snr-1 was not regulated by molybdate, oxygen, or nitrate.

146 of this interaction was found to be two ModE-molybdate per modA operator DNA.

147 e sensitivity of the combined pyrogallol red-molybdate precipitation/SDS-PAGE procedure is approximat

148 phase period when partial Mo(VI) centers of molybdate precursors are reduced to form {Mo(V)2(acetate

149 ssie Brilliant Blue (CBB) and Pyrogallol Red-molybdate (PRM) protein dye-binding assays to interferen

150 ch repressed modA-lac even in the absence of molybdate, protected the same region of modA operator DN

151 In summary, the pyrogallol red-molybdate protein precipitation procedure facilitates th

152 However, in the absence of p23 or molybdate, rapid disassembly of GR.hsp90 complexes appar

153 The factors affecting the molybdate reactive P (MRP) in these waters were analyzed

154 The stabilization was monitored by measuring molybdate-reactive silica and also by a combination of l

155 ined physical (centrifugation) and chemical (molybdate reactiveness) fractionation of leached P showe

156 DRP) in ice cores has been developed using a molybdate reagent and a 2-m liquid waveguide capillary c

157 fectively precipitated with a pyrogallol red-molybdate reagent from commonly used chromatographic buf

158 ModE, in the presence of 1 mM molybdate, repressed the production of plasmid-encoded M

159 gene, ModE, is a member of a unique class of molybdate-responsive DNA binding proteins.

160 nd conferred a modE phenotype (i.e., loss of molybdate-responsive gene expression) in vivo.

161 sion is impaired in strain defective for the molybdate-responsive ModE transcription factor.

162 regulators are similar to those of the known molybdate-responsive regulator ModE, while their DNA-bin

163 To determine if the molybdate-responsive transcription factor ModE is involv

164 Molybdate, selenate, chromate ("chromium VI"), arsenate,

165 quantitative extraction of (99m)Tc from the molybdate solution and complete release of (99m)Tc after

166 f these key species in the reduced acidified molybdate solutions and to observe the templation of the

167 y effective at separating (99m)TcO4 (-) from molybdate solutions.

168 aldoses, i.e., various molybdenum oxide and molybdate species, nickel(II) diamine complexes, alkali-

169 The modABC gene products constitute the molybdate-specific transport system in Escherichia coli.

170 peron in Escherichia coli, which codes for a molybdate-specific transporter, is repressed by ModE in

171 usly in euxinic marine waters after adding a molybdate spike and occur naturally in sulfidic geotherm

172 thelia followed by staining with an ammonium molybdate spray reagent revealed three phospholipid comp

173 nal nitric-oxide synthase (nNOS) exists as a molybdate-stabilized nNOS.hsp90 heterocomplex in the cyt

174 Because sodium molybdate stabilizes the AhR-HSP90 interaction and inhib

175 odABCD transcription even in the presence of molybdate, suggesting that ModE is a repressor.

176 and ModBC-A) to take up a greater amount of molybdate than a strain with ModBC-A alone.

177 tained by the reaction between phosphate and molybdate that is consequently reduced at the electrode

178 This Pi forms a complex with ammonium molybdate that is then reduced by ascorbic acid to provi

179 ichaelis constants for MgATP and sulfate (or molybdate), the dissociation constant of E.APS, and the

180 c gas-phase conversion of n-decane over iron molybdate, the product distribution correlates with the

181 ns 1 and 2 are essential for binding of ModE-molybdate to DNA, whereas the protected region 3 increas

182 the principle that phosphate ions react with molybdate to form redox molybdophosphate precipitates on

183 phosphate, the latter reacting with ammonium molybdate to form the ammonium phosphomolybdate complex

184 The binding of molybdate to ModE resulted in a decrease in fluorescence

185 Addition of molybdate to purified B. rapa anthocyanin resulted in an

186 winging door: allowing just enough space for molybdate to slip into the cell.

187 bstrate uptake studies, we reveal details of molybdate transport and the logistics of uptake systems

188 MtMOT1.3 shows molybdate transport capabilities when expressed in yeast

189 These results show that molybdate transport in E. coli is regulated by ModE, whi

190 se, indicating that there was a low-affinity molybdate transport system in this strain.

191 rt system might also serve as a low-affinity molybdate transport system in this strain.

192 tration, H. influenzae makes use of parallel molybdate transport systems (MolBC-A and ModBC-A) to tak

193 Members of the molybdate transporter family Molybdate Transporter type

194 und that MolBC-A functions as a low affinity molybdate transporter in its native environment.

195 Members of the molybdate transporter family Molybdate Transporter type 1 (MOT1) were identified in t

196 The Escherichia coli molybdate transporter, encoded by the modABCD operon, is

197 ABCD operon, which encodes the high-affinity molybdate transporter, is mediated by the ModE protein.

198 or the permease component of a high-affinity molybdate transporter.

199 RNA motif located upstream of genes encoding molybdate transporters, molybdenum cofactor (Moco) biosy

200 has to be provided by the host plant through molybdate transporters.

201 Because high levels of molybdate, tungstate, and selenite restored growth to wi

202 mporters of identical substrate specificity (molybdate/tungstate), and find that their interactions w

203 ve in the modA gene were unable to transport molybdate unless high levels of the anion were supplied

204 gene product is essential for high affinity molybdate uptake by the cell.

205 estingly, modC strains that are defective in molybdate uptake exhibit impaired anaerobic induction an

206 -electron paramagnetic resonance and in vivo molybdate uptake studies were used to test the impact of

207 es the protein components of a high affinity molybdate uptake system.

208 ction between the modA operator DNA and ModE-molybdate was 0.3 nM, and the K(d) increased to 8 nM in

209 H and XDH, increased when either selenite or molybdate was added to the culture medium.

210 on benzoate, was retarded unless 0.1 microM molybdate was added to the medium.

211 Molybdate was as effective in inhibiting Hg methylation

212 The Hsp90 antagonist molybdate was necessary to stabilize the interactions be

213 5 complexed with the inhibitors vanadate and molybdate were solved at 2.2 A resolution and compared t

214 (>2 mM) negatively affected the detection of molybdate, which eluted mainly in the dead volume, but h

215 ugh the formation of a complex with ammonium molybdate, which has an absorbance maximum at 700 nm.

216 modABCD transcription even in the absence of molybdate, which implies that this stretch of amino acid

217 et, other xenobiotics that are sulfated, and molybdate, which inhibits sulfate intestinal absorption,

218 e presence of hsp90 because it is blocked by molybdate, which prevents hsp90 dissociation.

219 ure of Escherichia coli ModE in complex with molybdate, which was determined at 2.75A from a merohedr

220 K, vnfH was expressed only in the absence of molybdate, with or without vanadate.

221 umber of steroid receptors, we reasoned that molybdate would be a useful tool in delineating the role