ライフサイエンスコーパス: morphant

1 ate published Morpholino-induced phenotypes (morphants).

2 endothelial Notch and HSC formations in evi1 morphants.

3 ring early primitive hematopoiesis in scube1 morphants.

4 mber of Rohon-Beard sensory neurons in cdk10 morphants.

5 biliary defects similar to those of the gpc1 morphants.

6 redicted roles in biomineralization in vegf3 morphants.

7 he cell junctions had disintegrated in zBves morphants.

8 ic acid are significantly decreased in rdhe2 morphants.

9 ntuated by L-leucine in both Rps19 and Rps14 morphants.

10 trated significant impairment of C-E in Zic3 morphants.

11 ants, rescues the growth retardation of orc1 morphants.

12 prematurely and rescues retraction in slit1b morphants.

13 ewer or truncated cilia are observed in Rfx2 morphants.

14 d body size, mirroring the phenotype of orc1 morphants.

15 and (c) pSmad2 levels were reduced in rbms3 morphants.

16 sylation and LLO levels were reduced in pmm2 morphants.

17 ally rescue the small eye phenotype of ZFRP2 morphants.

18 I restores pronephric duct formation in pkd2 morphants.

19 mbly is defective and disorganized in double morphants.

20 pitulated the laterality phenotype of alphaV morphants.

21 t partially overlap with those seen in ecscr morphants.

22 inding activity of alpha-dystroglycan in the morphants.

23 nockdown rescued neurogenesis defects in g9a morphants.

24 ectopic cytosolic CaMK-II expression in tbx5 morphants.

25 at is demethylated and up-regulated in dnmt3 morphants.

26 ly rescued the branching phenotype in sema5A morphants.

27 severely shortened or fail to form in Foxj1 morphants.

28 Lim3 and Pit1 expression in hypomorphic FGF3 morphants.

29 e number of cardiomyocyte was reduced in the morphants.

30 d signaling is significantly reduced in EGAP morphants.

31 mechanism for the vascular defects in magp1 morphants.

32 the bloodless phenotype in ZBP-89 zebrafish morphants.

33 while overexpressing notch rescues angptl1/2 morphants.

34 factor, are significantly reduced in elavl1a morphants.

35 ture and kidney cystogenesis in polycystin 2 morphants.

36 opic tubule gene expression in prkciota/zeta morphants.

37 bers, even in mild or intermediate phenotype morphants.

38 vinolin phenocopied the defects seen in scp2 morphants.

39 ns from slb/wnt11 mutants or wnt11 knockdown morphants.

40 h arch tissue can rescue cell death in foxi1 morphants.

41 cAMPS, partially rescued phenotypes of pde1a morphants.

42 tores spinal cord marker expression in Sall4 morphants.

43 scle myosin was markedly decreased in scube3 morphants.

44 ganglion cells was also diminished in cdk10 morphants.

45 nt extension movement was defective in Dhrs3 morphants.

46 ular morphogenesis appeared normal in scube1 morphants.

47 67 null mouse (bpck) and in zebrafish tmem67 morphants.

48 ion in a manner similar to that seen in lef1 morphants.

49 red sox2 and ngn1 transcript levels in cdk10 morphants.

50 enin and PCP activities is able to mimic the morphant accelerated cell motility phenotype.

51 In tmem88a morphants, activation of the WNT pathway exacerbates the

52 Cav-1alpha and -1beta morphants also exhibited disruption in the actin cytoske

53 trikingly, BMP misregulation seen in 3-OST-7 morphants also occurs in multiple cardiac noncontraction

54 were still able to rescue motor axons in smn morphants, although not as well as full-length PLS3.

55 Here, we use a combination of mutant and morphant analysis to ablate the function of each of the

56 Elevating Nodal levels in aplnra/b morphant and double mutant embryos is sufficient to resc

57 t this expression is diminished in both tbx5 morphant and hst embryos.

58 The morphant and overexpression phenotype represent a conver

59 entered the cell cycle relative to the stat3 morphant and significantly decreased the number and inte

60 sal axis curvature formation in polycystin 2 morphants and curly up polycystin 2 mutants.

61 e neurovascular characterization of mutants, morphants and drug-treated embryos.

62 own, but not Suv39h1 loss, phenocopied dnmt3 morphants and G9a overexpression provided a striking res

63 Similarly, zebrafish tbx5 morphants and mutants (heartstrings; hst) lack pectoral

64 synthesis and rRNA production in the cohesin morphants and partially restored phosphorylation of RPS6

65 are elevated in Na,K-ATPase alpha2 and Ncx4a morphants and that the defects in ciliary motility, KV f

66 idance is specifically disrupted in Lv-vegf3 morphants and these embryos fail to form skeletal elemen

67 ression, adcy8 and nrp1a morphants, or nrp1a morphants and transgenic DNGalpha(S) expression.

68 oc5 mutant zebrafish lines phenocopied exoc5 morphants and, strikingly, exhibited a virtual absence o

69 ranscripts are incorrectly spliced in Eif4a3 morphants, and demonstrate that inhibition of Xenopus Ry

70 Exogenous VEGFA rescues Tel1/ETV6 morphants, and depletion of VEGFA or its receptor, Flk1,

71 promoter were reduced in both dnmt3 and g9a morphants, and its knockdown rescued neurogenesis defect

72 rescue the body curvature phenotype of ZFRP2 morphants, and the eye size of the resultant morphants w

73 ervation of hair cells was reduced in ribeye morphants, and the reduction was correlated with depleti

74 d the developmental phenotypes of ciliopathy morphants, and this effect was rescued by human XPNPEP3

75 vacuolar protein sorting) family mutants and morphants, and we report here that knockdown of atp6ap2

76 rast, PMC migration is unaffected in Lv-fgfa morphants, and well-patterned but shortened skeletal ele

77 Low-dose Lgi1a MO knockdown fish (morphants) appear morphologically normal but are sensiti

78 ast (robo2) mutant and slit1a morphant arbors had more branch tips and greater arbor a

79 e-lapse imaging revealed that ast and slit1a morphant arbors stabilized earlier than wild-type, sugge

80 Ipsilateral misprojections in adcy8 morphants are corrected by the expression of an nrp1a re

81 e phenotypes observed in both Dhx34 and Nbas morphants are similar to defects in Upf1, Smg-5- or Smg-

82 he convergent extension defects in zebrafish morphants are similar to those found in planar cell pola

83 Lastly, CE defects in mcc morphants are suppressed by downstream activation of Rho

84 oretinograms (ERGs) were performed on dynein morphants at 5 to 6 days after fertilization (dpf).

85 ature was distorted in both fukutin and FKRP morphants, but not in dystroglycan morphants or control

86 escued the development of rad21 and nipbla/b morphants by boosting mTOR-dependent translation.

87 V cilia length, and KV morphogenesis in sdc2 morphants can be rescued by expression of fgf2 mRNA.

88 Knockdown of the ANLN gene in zebrafish morphants caused a loss of glomerular filtration barrier

89 etion of PDE1A aggravated phenotypes in pkd2 morphants, causing more severe body curvature, and human

90 By contrast, mesodermal Mypt1 morphant cells transplanted into wild-type hosts undergo

91 However, in CCDC78 morphants, Cep152 fails to localize to the deuterosome a

92 We also demonstrate that in CEP120 morphants, cilia are shortened in the neural tube and di

93 utonomous to CNC tissue, as the migration of morphant CNC can only be rescued by ADAM13 expression in

94 pended on Dnmt1 catalytic activity, as Dnmt1 morphants could be rescued by active zebra fish or human

95 Consistently, a comparison of published morphant defects with the Sanger Zebrafish Mutation Proj

96 m2 overexpression can partially rescue ccm2l morphant defects.

97 and ciliary organization analysis in tmem67 morphants did not support a global loss of planar polari

98 Morphants display a moderate decrease in the numbers of

99 decorin morphants displayed a pronounced shortening of the head-

100 Surprisingly, tubule cells in prkciota/zeta morphants displayed ectopic expression of the transcript

101 The morphants displayed shortened and abnormally branched mo

102 Although alphaV morphants displayed vascular defects, they also exhibite

103 at cultured muscle cells derived from Eif4a3 morphants do not contract, and fail to undergo calcium-d

104 RNA production were decreased in the cohesin morphant embryo cells.

105 The SmyD1 morphant embryos (embryos injected with morpholino oligo

106 proliferation and cartilage defects in chd7 morphant embryos and can lead to complete rescue of the

107 Conditional expression of Otx2 in these morphant embryos cell-autonomously rescues the formation

108 Morphant embryos displayed both cardiac and left/right p

109 Morphant embryos displayed cardiac abnormalities, includ

110 Single and double morphant embryos exhibited changes in splicing of overla

111 Morphant embryos had developmental abnormalities consist

112 enic DNGalpha(S)-expressing embryos or adcy8 morphant embryos have decreased levels of nrp1a and nrp1

113 ion of gene expression suggests that the APP morphant embryos have defective convergent-extension mov

114 Dvr1 morphant embryos have normal KV morphology and function,

115 his regard, laminin isolated from beta4GalT1 morphant embryos is poorly galactosylated, which may con

116 Cardiac myocytes in the morphant embryos lacked the intercalated disks that were

117 Morphant embryos manifest craniofacial defects, impaired

118 the developmental processes affected in FKRP morphant embryos may lead to a better understanding of t

119 FKRP morphant embryos showed a spectrum of phenotypic severit

120 and the retinal neuroblasts observed in the morphant embryos suggests that obscurin A-mediated small

121 Myofibril organization in the morphant embryos was severely disrupted.

122 Morphology and development of single morphant embryos were strikingly normal; however, muscle

123 BP1) was reduced in nipbla/b, rad21 and smc3-morphant embryos, a pattern indicating reduced translati

124 ns in adcy1, adcy8, nrp1a, sema3D, or sema3E morphant embryos, as well as in sema3D mutant embryos.

125 In tag1 morphant embryos, FBMN migration is specifically blocked

126 Integrin function is compromised in magp1 morphant embryos, suggesting that reduced integrin-matri

127 s are affected similarly in all three single morphant embryos, with an inability to extend protrusion

128 onses to, mesoderm-inducing signals in lin28 morphant embryos.

129 OR111-7 transgene-expressing neurons in DCC morphant embryos.

130 ecreased glut1 transcript expression in akt2 morphant embryos.

131 he inhibition of apoptosis and rescue of the morphant embryos.

132 ingly similar defects to those seen in disc1 morphant embryos.

133 erm is severely abnormal in lin28 knockdown (morphant) embryos.

134 rexpression was sufficient to rescue Swap70b morphants, establishing Swap70b as a novel member of the

135 naffected in mitf mutants, as well as in otx morphants, even in regions lacking RPE.

136 pondin repeat (TSR) domain alone into sema5A morphants exclusively rescued delay in ventral motor axo

137 Late gastrula-stage FoxH1 morphants exhibit delayed mesoderm and endoderm marker g

138 arch osteochondrogenic progenitors, and that morphants exhibit diminished and dysmorphic arch cartila

139 Moreover, cdk10 morphants exhibited a marked loss of huC-positive neuron

140 Swap70b morphants exhibited broader and shorter body axis but ce

141 The Lrrc10 morphants exhibited cardiac functional defects as eviden

142 hants had similar brain abnormalities, kdm6a morphants exhibited craniofacial phenotypes, whereas kdm

143 The kmt2d morphants exhibited severe abnormalities in all tissues

144 The dnajb1a morphants exhibited underdeveloped cataractous lenses wi

145 th reduced expression of the cftr gene (Cftr morphants) exhibited reduced respiratory burst response

146 ependent hair-cell loss occurred in neurog1a morphants exposed to either agonist, and the loss was co

147 In Zic morphants expression of roof plate markers, including lm

148 d less stable protrusions, and those in myh6 morphants extended fewer protrusions per cell.

149 nscription analysis revealed that nanog-like morphants fail to develop the extraembryonic yolk syncyt

150 Within the retina of lmx1b morphants, Fgf activity is transiently up-regulated and

151 Analysis of rx3 chk mutant/rx3 morphant fish and EdU pulse-chase studies reveal that rx

152 monstrate atp1b2b levels are reduced in eya4 morphant fish and that morpholino oligonucleotides targe

153 re viable, and by 4 days post-fertilization, morphant fish display multiple defects in organ systems

154 Consequently, tgfbeta1a morphant fish displayed phenotypical similarities with p

155 eya4 morphant fish embryos had reduced numbers of hair cells

156 In general, the fukutin morphant fish had the most severe skeletal muscle and ey

157 Interestingly, these morphant fish having human FKRP mutations showed a wide

158 r some of the effects of PINK1 knockdown, as morphant fish show elevated GSK3beta activity and their

159 All morphant fish showed muscle damage and vascular abnormal

160 hout the central nervous system of high-dose morphant fish, accounting for the size reduction of neur

161 sregulation of pax2 in the developing eye of morphant fish, suggesting that Nlz1 and Nlz2 act upstrea

162 In actn2 morphant fish, the Z-disc defect sequentially affects ca

163 rexpression rescued these phenotypes in eya4 morphant fish.

164 e to innervating neurons, I exposed neurog1a morphants-fish whose hair-cell organs are devoid of affe

165 afish paralogs kdm6a and kdm6al, we analyzed morphants for developmental abnormalities in tissues tha

166 Zebrafish morphants for either gene develop pigmentary defects and

167 Morphants for rbms3 displayed reduced proliferation of p

168 Zebrafish morphants for snx14 showed dramatic loss of cerebellar p

169 likely to form protrusions, those in tnnt2a morphants formed less stable protrusions, and those in m

170 Zebrafish adcy1b morphants had no FM1-43 dye uptake and lacked startle re

171 ited craniofacial phenotypes, whereas kdm6al morphants had prominent defects in heart development.

172 Although the kdm6a and kdm6al morphants had similar brain abnormalities, kdm6a morphan

173 Further, otoferlin morphants had uninflated swim bladders.

174 pparently not in the neural crest, and nrp1A morphants have cardiac development defects.

175 Chd7 morphants have impaired migration of neural crest cells

176 High-dose Lgi1a morphants have morphological defects which persist into

177 Sema3D morphants have subsequent complex congenital heart defec

178 uced fluorescence intensity from NADH in the morphant heart was observed in live zebrafish embryos as

179 Morphant hearts were hypoplastic and dysmorphic and exhi

180 genetic interaction between arl13b and sec10 morphants in cilia-dependent phenotypes.

181 nts was significantly increased over that of morphants in which ZFRP2 had been depleted.

182 of two cardiac genes was deregulated in the morphants including an increase in atrial natriuretic fa

183 ions of hepatoblasts are detectable in rargb morphants, indicating Rargb acts during hepatic specific

184 ICD), rescued the expansion of MCCs in mecom morphants, indicating that mecom acts upstream to induce

185 embryos is abnormally widened in beta4GalT1 morphants, indicative of abnormal convergent extension.

186 protein accumulates in hair cells of CaMK-II morphants, indicative of defective recycling and/or exoc

187 Bacterial burden in Cftr morphants infected with a P. aeruginosa DeltaLasR mutant

188 Similar to what was observed in the glut1 morphants, inhibiting the expression of the proapoptotic

189 Here we show that mutants and morphants involving other V-H(+)-ATPase subunits also de

190 Decreased hepatocyte proliferation in copeb morphants is accompanied by upregulation of the cell cyc

191 The motility phenotype found in the morphants is not observed in embryos in which the Wnt/be

192 failure to form the PAC in netrin 1a or dcc morphants is phenocopied by laser ablation of motoneuron

193 o rescue motor neuron pathology in zebrafish morphants lacking Gle1.

194 -gamma) or IFN-alpha signaling and zebrafish morphants lacking IFN-gamma and IFN-varphi activity had

195 ryos shows defective calcium release in bin1 morphants, linking the presence of abnormal triads to im

196 veral lysosomal glycosidases was observed in morphant lysates, consistent with the reduction in phosp

197 lin 2 and alpha-actinin 1 did not affect smn morphant motor axon outgrowth.

198 sed embryonic skeletal muscle paralysis, and morphant motor neurons had aberrant morphology including

199 ypomorphs and isoform-specific (medium/long) morphants not only recapitulate this phenotype but also

200 Morphants of beta2 CaMK-II (camk2b2), but not the beta1

201 death of the periocular mesenchyme in Lmx1b morphants, optic vesicle morphogenesis is largely restor

202 and FKRP morphants, but not in dystroglycan morphants or control fish.

203 ignature and myc overexpression in angptl1/2 morphants or mib restored HSPCs formation.

204 ding domain mutations, FoxH1 splice-blocking morphants or other Nodal pathway mutants, and it cannot

205 Noninvasive live imaging of Rac2 morphants or Rac2D57N zebrafish larvae demonstrates an e

206 enic DNGalpha(S) expression, adcy8 and nrp1a morphants, or nrp1a morphants and transgenic DNGalpha(S)

207 if the first hair cells are missing (atoh1b morphant), otolith formation is severely disrupted and d

208 l matrix formation and calcium deposition in morphant otoliths.

209 The FoxH1 morphant phenotype is much more severe than that of zebr

210 However, the morphant phenotype observed following specific depletion

211 Similar NBP and Numb morphant phenotype such as impaired convergence and exte

212 The overall morphant phenotype was also obtained by co-injecting sub

213 This Grk5 morphant phenotype was associated with an overall decrea

214 The morphant phenotype was efficiently rescued by zebrafish

215 The morphant phenotype was rescued when mRNA encoding murine

216 of TGF-beta signalling phenocopies the ltbp3-morphant phenotype whereas expression of a constitutivel

217 F1A2 wild-type mRNA successfully rescued the morphant phenotype, but mutant RNA did not.

218 so show that wild-type human APP rescues the morphant phenotype, but the Swedish mutant APP, which ca

219 nhibition of Bmp signaling rescues the rargb morphant phenotype, indicating Rargb functions upstream

220 sulted in a failure to rescue the cav-1alpha morphant phenotype, verifying an essential role for Cav-

221 sion of vhnf1 mRNA led to rescue of the pk1a morphant phenotype.

222 s with a mixture of MOs reproduces the FoxH1 morphant phenotype.

223 n analysis of Cby's embryonic expression and morphant phenotypes in Xenopus laevis.

224 n Project revealed that approximately 80% of morphant phenotypes were not observed in mutant embryos,

225 Morphant phenotypes were rescued by injection of homotyp

226 Many, but not all, morphant phenotypes were significantly reversed by the W

227 hands were essential for PLS3 rescue of smn morphant phenotypes, and mutation of the Ca(2+)-binding

228 , and human PDE1A RNA partially rescued pkd2 morphant phenotypes, pronephric cysts, hydrocephalus, an

229 bitor) resulted in a partial rescue of smad7 morphant phenotypes, suggesting scl2a10/glut10 functions

230 pression provided a striking rescue of dnmt3 morphant phenotypes, whereas Suv39h1 overexpression fail

231 Morphant photoreceptor connecting cilia were swollen, bu

232 3 inhibits normal PMC ingression and foxN2/3 morphant PMCs do not organize in the blastocoel and fail

233 rystallin isoform mRNAs lost from the eif3ha morphant polysomes, revealing a mechanism by which lens

234 We establish that, in FoxA2-FoxA3 double morphants, precursors of axial tissues are correctly ind

235 In the perlecan morphants, primary intersegmental vessel sprouts, which

236 In zebrafish, tbc1d23 morphants replicated the human phenotype showing hindbra

237 lasts, activation of Notch signaling in hey2 morphants rescues HSC formation in zebrafish embryos.

238 Def6a/Swap70b morphants resemble Ppt/Slb double mutant embryos suggest

239 The increased OPC numbers in nf1 morphants resulted from increased proliferation, as dete

240 Microarray analyses of apc mutants and Rdh5 morphants revealed a profound overlap in the transcripti

241 ic Swap70b expression robustly rescued Wnt11 morphants, RhoA overexpression was sufficient to rescue

242 Probing FoxH1 morphant RNA by microarray, we identified a cohort of fi

243 The perlecan morphants show a severe myopathy characterized by abnorm

244 tivity is transiently up-regulated and these morphants show defective naso-temporal patterning.

245 Danio rerio (Zebrafish) tbx20 morphants show defects in the migration patterns of moto

246 The chd7 morphants show elevated expression of several potent cel

247 These zebrafish morphants show Rac hyperactivation and progress faster t

248 Characterization of the gne knockdown morphants showed a significantly reduced locomotor activ

249 Consistent with these results, slc2a10 morphants showed decreased respiration and reduced TGFbe

250 racked in real-time, individual DFCs of both morphants showed defects in DFC migration, preventing th

251 The FKRP morphants showed phenotypes involving alterations in som

252 and, accordingly, tuba start and splice site morphants showed various ciliary mutant phenotypes in th

253 nd enhanced phenotypic aberrations in double morphants suggest that the genes interact genetically.

254 nd that the sarcomere remains intact in tcap morphants, suggest that defective sarcomere assembly doe

255 ved in fast muscle fibers of pgam2 zebrafish morphants, suggesting affected myoblast fusion.

256 idline and gut looping is perturbed in rargb morphants, suggesting Rargb affects lateral plate mesode

257 is abolished in Na,K-ATPase alpha2 and Ncx4a morphants, suggesting their essential role in KV ciliary

258 on of constitutively active TOR rescued EGAP morphants, suggesting TOR is a direct or indirect endoge

259 d Notch signalling [mindbomb mutant or Su(H) morphant], supernumerary hair cells develop and otolith

260 stand the in vivo function of Pen-2, we used morphant technology available in zebrafish and transient

261 to be significantly higher in zebrafish Cftr morphants than in controls, but this phenomenon was not

262 In Zic1 and/or Zic4 morphants the ventricle does not open properly, but rema

263 Consequently, in Wnt1 morphants, the blastopore is located at the border of th

264 In pmm2 morphants, the free glycan by-products of LLO cleavage i

265 re present in muscle fibers of rbfox1l/rbox2 morphants, they are substantially reduced in number and

266 Exposure of the gpc1 morphants to cyclopamine, a Hedgehog antagonist, partial

267 stigation of the craniofacial defects in the morphants uncovered striking changes in the timing and l

268 splayed phenotypical similarities with pcsk7 morphants, underscoring the importance of this cytokine

269 Knockdown of Lrrc10 in zebrafish embryos (morphants) using morpholinos caused severe cardiac morph

270 In 3-OST-7 morphants, ventricular contraction can be rescued by ove

271 guingly, overexpression of glut1 in the akt2 morphants was also able to rescue these embryos.

272 The phenotype of ZFRP2 knockdown morphants was characterized by immunohistology and histo

273 ormal; however, muscle development in double morphants was severely disrupted.

274 morphants, and the eye size of the resultant morphants was significantly increased over that of morph

275 etinas and ciliated cells of these zebrafish morphants were analyzed by immunohistochemistry and tran

276 When the morphants were exposed to several drugs with antioxidant

277 Cftr morphants were infected with P. aeruginosa or other bact

278 of tfpi, and hematopoietic defects in adtrp1 morphants were rescued by tfpi overexpression.

279 Both gonzo mutants and scap morphants were resistant to steatosis in response to 2%

280 ted after knockdown of Bves, and these zBves morphants were sensitive to osmotic stress.

281 ize was also smaller in the fukutin and FKRP morphants when compared with dystroglycan knockdown fish

282 d size and neuroanatomical defects of kctd13 morphants, whereas suppression or overexpression of CEP2

283 lack cardiac contractions and flow, and myh6 morphants, which lack atrial contraction and exhibit red

284 ent animals, including erbb2 mutants, tnnt2a morphants, which lack cardiac contractions and flow, and

285 Dnmt1p target genes can be reimposed in xDMO morphants with an mRNA encoding a catalytically inactive

286 tterning defects at later stages, since Zic3 morphants with moderate to severe C-E defects exhibited

287 Second, co-injection of morphants with the long human TMEM260 mRNA rescued CNS p

288 Morphant zebrafish also exhibited elevated levels of apo

289 In addition, Mmp21-mutant mice and mmp21-morphant zebrafish displayed heterotaxy and abnormal car

290 toe1-morphant zebrafish displayed midbrain and hindbrain dege

291 Morphant zebrafish embryos also exhibited increased apop

292 ts in cells lacking BBS1, BBS4, and OFD1, in morphant zebrafish embryos, and in induced neurons from

293 g strategy using stage-matched WT and eif3ha morphant zebrafish embryos.

294 fy motor axon outgrowth phenotypes in an smn morphant zebrafish model of SMA.

295 Confocal microscopy of morphant zebrafish revealed olfactory placodes with defe

296 Moreover, when expressed in mfsd2aa-morphant zebrafish, mutants failed to rescue microcephal

297 In contrast to what has been reported in morphant zebrafish, zap70(y442) homozygous mutant zebraf

298 defects were seen in both knockout mice and morphant zebrafish.

299 ion and mineralization were delayed in panx3 morphant zebrafish.

300 nd examined the skeletal phenotypes of panx3 morphant zebrafish.