1 msDNA is synthesized from an RNA template by the retron-
2 nstrate interference with mismatch repair
by msDNA, we show that the addition of a plasmid containing
3 e production of a small satellite DNA
called msDNA.
4 ly obscure RNA-DNA satellite molecule
called msDNA.
5 msDNA, the RT, and the retron are
designated msDNA-Ec48, RT-Ec48, and retron-Ec48, respectively.
6 Multicopy single-stranded
DNA (
msDNA) molecules consist of single-stranded DNA covalent
7 ucture of the multicopy single-stranded
DNA (
msDNA) produced by the RT.
8 e DNAs called multicopy single-stranded
DNA (
msDNA).
9 Previously, we showed that retrons
encoding msDNAs with mismatched base pairs are mutagenic when pre
10 on using an RNA (the primer-template RNA
for msDNA) as a template and the 3' end of a nick in the chr
11 A strand and five bases of the RNA strand
in msDNA-Ne144.
12 tides; it is the longest RNA among the
known msDNAs.
13 We also show that in mutS
mutants,
msDNA does not increase the frequency of either mutation
14 The presence of this truncated copy
of msDNA is strong evidence of retrotransposition in N. exe
15 This partial copy
of msDNA is very likely a retrotransposed sequence that was
16 ructure of the msr gene, the RNA molecule
of msDNA-Ec48 is predicted to be composed of 119 ribonucleo
17 equences match only part of the sequences
of msDNA and may have become incorporated into the chromoso
18 he gene msr (which encodes the RNA strand
of msDNA) appears to be duplicated and flanks both sides of
19 he gene msd (which encodes the DNA strand
of msDNA).
20 the mutagenic and recombinogenic effects
of msDNAs are due to titrating out MutS protein.
21 the mutagenic and recombinogenic effects
of msDNAs.
22 The DNA moieties
of msDNAs have characteristic stem-loop structures, and mos
23 The resulting
products (
msDNA) consist of RNA and single-stranded DNA, sharing h
24 NA of 48 nucleotides in length, the
shortest msDNA thus far identified from natural sources.
25 In this study we show that
such msDNAs, in a similar manner to genetic defects in mismat
26 The msDNA was found to consist of a single-stranded DNA of 4
27 The msDNA, the RT, and the retron are designated msDNA-Ec48,
28 also contains several partial copies of
the msDNA sequence as revealed by DNA hybridization experime
29 This msDNA is composed of a 144 base, single-stranded DNA tha
30 um is a set of repeated sequences related
to msDNA.
31 ealed by DNA hybridization experiments
using msDNA as a probe.