ライフサイエンスコーパス: multiplex PCR

1 tested to perform high-throughput nanoliter multiplex PCR.

2 -well design of the SlipChip were tested for multiplex PCR.

3 at used broth enrichment prior to sequential multiplex PCR.

4 almonella DNA microarray, and prophage-based multiplex PCR.

5 high-resolution subtyping not possible with multiplex PCR.

6 interference that is common to conventional multiplex PCR.

7 eptibility, API 20S assay, and genotyping by multiplex PCR.

8 e dismutase (sodA) gene for development of a multiplex PCR.

9 that far exceed the capacity of traditional multiplex PCR.

10 -linking, chromatin immunoprecipitation, and multiplex PCR.

11 PCR amplification, which may be enhanced by multiplex PCR.

12 nations in isolated clones are determined by multiplex PCR.

13 logic path toward maximizing the benefit of multiplex PCR.

14 aminoglycoside-modifying enzyme genes using multiplex PCR.

15 the repertoires generated by 5'-RACE PCR and multiplex PCR.

16 as investigated in 43 isolates of amoebae by multiplex PCR.

17 species identification using a 16S rRNA gene multiplex PCR.

18 omposition of sequence libraries prepared by multiplex PCR.

19 esence of viral infection was ascertained by multiplex PCR.

20 sitive, and amenable to both single-step and multiplex PCRs.

21 ultiple sexually transmitted infections, and multiplex PCRs.

22 pathogenic fungi can be identified with two multiplex PCRs.

23 Eight multiplex PCRs, 32 targeting serotype-determining capsul

24 good agreement between our single reaction, multiplexed PCR/AFM data, and data from 20 individual si

25 nalyzed by single-cell reverse transcriptase multiplex PCR after patch clamp and was compared with th

26 The targets were generated by multiplex PCR, allowing simultaneous amplifications of 4

27 Results of the multiplex PCR also indicated that the ehxA gene for ente

28 next-generation sequencing, termed anchored multiplex PCR (AMP), that is compatible with low nucleic

29 Here, we carried out a combination of multiplex PCR, amplicon quantification, and next generat

30 edical Products), which uses target-enriched multiplex PCR amplification followed by liquid array ide

31 pproach used in this study involves one-tube multiplex PCR amplification of six target bacterial viru

32 Fluorescent output of multiplexed PCR amplification could also be imaged with

33 iquid samples and analyzes the samples using multiplexed PCR amplification coupled with microsphere a

34 The multiplexed PCR amplified the multiply repeated IS481 B.

35 n Diego, CA, USA, www.sequenom.com) to assay multiplex PCR-amplified single-stranded cDNAs and easily

36 d positive and negative predictive values of multiplex PCR analyses were 87, 96, 94, and 93%, respect

37 A combination of oligonucleotide arrays, multiplex PCR analysis and single-nucleotide polymorphis

38 Firstly, multiplex PCR analysis indicated that the novel strain b

39 By combining patch-clamp and RT-multiplex PCR analysis of individual neurons in mouse br

40 Multiplex PCR analysis revealed the types of mutants to

41 All of these strains were serotyped, and multiplex PCR analysis was used to ascertain the presenc

42 Nested multiplex PCR analysis was used to type four independent

43 s were identified to the species level using multiplex PCR and 16S rRNA gene sequencing.

44 The method employs multiplex PCR and a novel pooling strategy to minimize t

45 We present a method based on multiplex PCR and array detection of amplicons to assay

46 ymorphic markers in the same reaction with a multiplex PCR and base extension reaction.

47 V strains of each species type and tested by multiplex PCR and culture.

48 cing method that couples microfluidics-based multiplex PCR and deep sequencing (mmPCR-seq) to uniform

49 Using multiplex PCR and DNA sequencing, we identified a single

50 and Escherichia coli based on triple-tagging multiplex PCR and electrochemical magneto genosensing on

51 plasmid-borne ermC gene were monitored by a multiplex PCR and gene-specific internal probing assay.

52 otide polymorphisms in 19 candidate genes by multiplex PCR and hybridization to an immobilized probe

53 mpacted subsequent HPV detection in both the multiplex PCR and Linear Array assays.

54 x PCR and standard Linear Array to 0.881 for multiplex PCR and modified Linear Array.

55 e then examined all patients by microfluidic multiplex PCR and next-generation sequencing for all 27

56 HPV status agreements ranged from 0.615 for multiplex PCR and standard Linear Array to 0.881 for mul

57 ossible sequence variations, using extensive multiplex PCR and two-dimensional electrophoretic separa

58 even serotype 6C isolates were identified by multiplex PCR and/or Quellung reaction.

59 signature design from genome-wide datasets, multiplex-PCR and detection by single-base extension and

60 By multiplexing PCR and pooling multiplexed reactions toget

61 de-modifying enzymes (AMEs) were detected by multiplex PCR, and clonal relationships were determined

62 accessory gene regulator group genotyping by multiplex PCR, and identification of toxin and potential

63 Newer technologies, such as multiplex PCR, and novel diagnostic platforms that incor

64 The broth enrichment, conventional multiplex PCR, and real-time PCR approaches used in this

65 and environmental samples and can be used in multiplex PCR applications.

66 A rapid multiplex PCR approach was developed to detect the bft g

67 polymerase chain reaction [PCR]), including multiplex PCR, as well as and microscopy for protozoal i

68 ortrait Staph ID/R blood culture panel (BCP) multiplex PCR assay (Great Basin Scientific, Salt Lake C

69 sion of the FilmArray Respiratory Panel (RP) multiplex PCR assay (Idaho Technology, Inc., Salt Lake C

70 This high-throughput multiplex PCR assay allowed simple and accurate typing o

71 We then used this information to design a multiplex PCR assay based on the simultaneous amplificat

72 MuPlex is uniquely designed for large-scale multiplex PCR assay design in an automated high-throughp

73 cal specimens from Bangladesh were used, the multiplex PCR assay detected 95% (21 of 22) of Giardia m

74 mbination of murG and mur-2(ed) primers in a multiplex PCR assay differentiated E. hirae from E. dura

75 s were used to construct a semiquantitative, multiplex PCR assay for BV based on detection of 3 posit

76 A rapid real-time multiplex PCR assay for detecting and differentiating Bo

77 ers functioned well when incorporated into a multiplex PCR assay for diverse virulence-associated gen

78 A multiplex PCR assay for GBS capsular gene typing was als

79 A multiplex PCR assay for identification of each of these

80 Compared to the previous assays, this single multiplex PCR assay had higher molecular sensitivities f

81 n with primers to detect PVL, we developed a multiplex PCR assay specific for the identification of U

82 dge, this is the first report of a real-time multiplex PCR assay that can be used for the simultaneou

83 rep-B-Latex agglutination method and a novel multiplex PCR assay that detected all 10 GBS capsular ty

84 A multiplex PCR assay that detects the four commonest caus

85 , a previously described Ad species-specific multiplex PCR assay that used primers to the Ad hexon ge

86 terial genomic data, we developed a one-step multiplex PCR assay to identify Salmonella and simultane

87 For this purpose, we have designed a single multiplex PCR assay to simultaneously amplify 95 diagnos

88 A real-time, multiplex PCR assay using HRM was designed for the detec

89 Direct multiplex PCR assay using vanA and vanB primers, which p

90 A multiplex PCR assay was developed by using primers to th

91 A real-time multiplex PCR assay was developed to allow detection of

92 The multiplex PCR assay was found to be a specific and sensi

93 The multiplex PCR assay was specific at discriminating each

94 Furthermore, a multiplex PCR assay was validated for rapid molecular di

95 Genetically tagged UPEC and a multiplex PCR assay were employed to investigate the dis

96 Our multiplex PCR assay will permit rapid, accurate, and cos

97 nd, we have combined a fluorogenic real-time multiplex PCR assay with four sets of degenerate PCR pri

98 Finally, a novel multiplex PCR assay, based on random amplified polymorph

99 that 97% were correctly identified using the multiplex PCR assay.

100 olates were all classified as type A using a multiplex PCR assay.

101 ved Shigella invasion antigen, IpaH3, into a multiplex PCR assay.

102 cterial, 3 viral, and 3 parasite) commercial multiplex PCR assay.

103 First, a semiquantitative, multiplexed PCR assay (BV-PCR) and the Affirm VPIII Gard

104 Second, a multiplexed PCR assay detecting Candida albicans and Can

105 characterizing HCMV strains and found that a multiplexed PCR assay using primers based upon these STR

106 agnostics LLC, Salt Lake City, UT), a highly multiplexed PCR assay, can identify 24 etiologic agents

107 MuPlex designs a set of multiplex PCR assays designed to cover as many of the in

108 Qiagen Spin blood kit held as the constant, multiplex PCR assays detect more HPV-positive specimens

109 However, the design of a multiplex PCR assays is computationally challenging beca

110 Subsequently, simple multiplex PCR assays were designed on the basis of these

111 New multiplex PCR assays were developed for mating-type dete

112 ime human papillomavirus (HPV) type-specific multiplex PCR assays were developed to detect HPV DNA in

113 Multiplex PCR assays were developed to identify Actinoba

114 These multiplex PCR assays, based on prophage-like elements an

115 d HPV59 in type- and gene-specific real-time multiplex PCR assays.

116 , Wageningen, the Netherlands) and published multiplex PCR assays.

117 uPlex that aids researchers in the design of multiplex PCR assays.

118 When analyzed by multiplex PCR at the single-cell level, normal splenic P

119 We have developed a strategy for multiplex PCR based on PCR suppression.

120 Here, we describe the development of a multiplex PCR, based on variation within the capsule loc

121 Here, we designed and validated a novel multiplex PCR-based assay for STc648 that took advantage

122 Thus, this novel multiplex PCR-based assay should enable any laboratory e

123 We used a multiplex PCR-based assay to simultaneously detect polym

124 To address this topic, we devised a multiplex PCR-based assay, termed TAR (telomere-associat

125 nts with blood culture pathogens on a rapid, multiplex PCR-based blood culture identification panel (

126 The multiplex PCR-based NGS is useful and robust to acquire

127 A novel multiplex PCR-based NGS was developed to gather whole ge

128 Using a highly multiplexed PCR-based target enrichment method (RainDanc

129 Simple, multiplexed, PCR-based barcoding of DNA for sensitive mu

130 The multiplex PCR can distinguish 17 individual serotypes in

131 The RMA utilizes improved multiplex PCR chemistry (EraGen MultiCode-PLx technology

132 The NanoChip400 system uses multiplex PCR chemistry and electronic microarray detect

133 y viruses uses an expanded genetic alphabet, multiplex PCR chemistry, and microsphere flow cytometry

134 In the remaining eight tumors, multiplex PCR demonstrated that the observed heterozygos

135 fication artifacts typically associated with multiplex PCR derived from the use of many primer pairs

136 The multiplex PCR described therefore represents a simple an

137 trol that was undetectable by very sensitive multiplex PCR, despite increasing antibodies.

138 The multiplex PCR detected organisms in 26 of the 30 true-po

139 Multiplex PCR detected potentially significant bacteria

140 leic acid amplification system that provides multiplexed PCR detection of the influenza A, influenza

141 ampC multiplex PCR differentiated the six plasmid-mediated am

142 rising an online primer design tool, a novel multiplex PCR enrichment protocol, optimized library pre

143 efore, we developed the Pneumoplex assays, a multiplex PCR-enzyme hybridization assay (the standard a

144 Therefore, we developed a multiplex PCR-enzyme hybridization assay, the Adenoplex,

145 MPD can successfully design multiplex PCR experiments suitable for next-generation s

146 rce software that can design next-generation multiplex PCR experiments that ensures primers are uniqu

147 Multiplex PCR experiments were performed on isolates to

148 A rapid "mega"-multiplex PCR (FilmArray gastrointestinal panel; BioFire

149 ventional methods in the first season and by multiplex PCR (FilmArray) in the second season.

150 ix to eight diagnostic sequences/organism by multiplex PCR; fluorescent labeling of the amplicons via

151 ygosity mapping, whole-exome sequencing, and multiplex PCR followed by next-generation sequencing.

152 We then developed multiplex PCR for amplification of these 15 loci in four

153 either random nucleic acid amplification or multiplex PCR for GAS detection.

154 Multiplex PCR for genus and species determination of ent

155 Fingerprinting by multiplex PCR for IS900 integration loci clustered 67 of

156 tuberculosis isolates representing different multiplex PCR for IS900 loci (MPIL) or amplified fragmen

157 sting, pulsed-field gel electrophoresis, and multiplex PCR for phylotyping to determine their resista

158 tic signatures, along with a newly developed multiplex PCR for rapid differentiation between "wild-ty

159 Our method relies on multiplex PCR for targeted enrichment of viral genomes f

160 We developed a real-time fluorescence-based multiplex PCR for the detection of all six of the curren

161 Furthermore, we have developed a new multiplex PCR for the detection of serovars 1 to 3, 5 to

162 We have developed a multiplex PCR for the detection of these Mycoplasmatales

163 These direct selection methods supersede multiplex PCR for the large-scale analysis of genomic re

164 This report describes the development of a multiplex PCR for the purpose of identifying family-spec

165 udy proposes an authentication protocol with multiplex PCR for three species of snappers (Lutjanus pu

166 rology and nasopharyngeal swab specimens for multiplex PCR from case patients and controls.

167 nd obtained oropharyngeal swab specimens for multiplex PCR from case patients, and serum for serology

168 Consequently, SmaI-multiplex PCR has the potential to be used in routine cl

169 Multiplex PCR has the potential to rapidly identify bloo

170 Real-time multiplex PCR has the potential to serve as an adjunct t

171 Multiplex PCR identified T. nativa from the bear meat, a

172 One multiplex PCR identifies serogroup D, A, and B and Vi-po

173 59 months, we compared organism detection by multiplex PCR in IS and nasopharyngeal/oropharyngeal (NP

174 y-determining region 3 length analysis using multiplex PCR in purified peripheral blood CD8 T cells o

175 Amplification is initiated by a multiplex PCR in this case with 170 primer pairs.

176 and schematic sequence-based system of seven multiplex PCRs, in a sequence order based upon Active Ba

177 dvantages of using the SlipChip platform for multiplex PCR include the ability to preload arrays of d

178 We perform multiplex PCR inside a capillary, transfer the amplified

179 Multiplex PCR is a key technology for an endless list of

180 Melting-point analysis of real-time multiplex PCR is a rapid, sensitive, specific, and inexp

181 Multiplex PCR is defined as the simultaneous amplificati

182 Thus, a multiplex PCR is designed by which known mutations in C4

183 Despite these difficulties, multiplex PCR is frequently used in applications such as

184 The multiplex PCR-LDR assay goes beyond other PCR-based assa

185 Application of the multiplex PCR-LDR assay will provide the sensitivity and

186 Using the K- ras gene as a model system, multiplex PCR/LDR followed by hybridization to prototype

187 Multiplex PCR/LDR was able to identify mutations in soli

188 ns determined by both dideoxy-sequencing and multiplex PCR/LDR.

189 We have developed a multiplex PCR-ligase detection reaction (LDR) assay that

190 A multiplex PCR-ligation detection reaction (PCR-LDR) assa

191 A multiplex-PCR Luminex xMAP bead probe fluid array using

192 This multiplex PCR-Luminex assay enables sensitive, specific,

193 Using a multiplex PCR, major changes were detected in 'on/off' s

194 with carbapenemase-producing organisms using multiplex PCR may help hospitals to improve infection co

195 A multiplex PCR method coupled with allele-specific oligon

196 A multiplex PCR method for determination of capsule types

197 A multiplex PCR method has been developed to differentiate

198 The pipette optimized multiplex PCR method has been employed in the final phas

199 h, high error rate, and undetermined bias of multiplex PCR method have hindered broader application o

200 This multiplex PCR method provides a rapid, simple, and relia

201 ers targeting prp and cps were combined in a multiplex PCR method that defines species using the tlh

202 This study used a nested multiplex PCR method to detect three periodontal pathoge

203 A multiplex PCR method was developed to identify simultane

204 We have tested a multiplex PCR method which shows the presence or absence

205 Resistance genes were confirmed by a multiplex PCR method with the vanC gene detected in all

206 This report describes a multiplex PCR method, the Mycobacterial IDentification a

207 ains of known genotype were used to test our multiplex PCR method, which showed 100% sensitivity and

208 r, only 1 of these 12 was Vi negative by the multiplex PCR method.

209 Isolates were characterized by using multiplex PCR methodology to determine structural types

210 ed to identify 8 serovars (9 genovars) in 12 multiplex PCR mixes on 11 S. enterica strains.

211 ing microbiologic, serologic, and sequential multiplex PCR (MP-PCR) techniques to serotype the isolat

212 A six gene multiplex PCR (mPCR) assay, designed to effectively diff

213 The multiplex PCR (mPCR) distinguished between all previousl

214 A multiplex PCR (mPCR) was constructed based on these gene

215 Capsular serotypes were determined by multiplex PCR, multibead assay, or latex agglutination.

216 ating DNA methylation of multiple loci via a multiplex PCR (multiplexed-scAEBS).

217 identification immunoassays, and orthogonal, multiplexed PCR (nucleic acid) amplification and detecti

218 nd serotype 6C was differentiated from 6A by multiplex PCR of DNA extracts by using appropriate prime

219 Multiplex PCR of IS900 integration loci (MPIL) and ampli

220 of oligonucleotides to amplicons produced by multiplexed PCR of genomic DNA, individual microspheres

221 Multiplex PCR offers a rapid, sensitive, and easy method

222 We developed two multiplex PCRs, one end-point and one real-time with mel

223 Primer approximation multiplex PCR (PAMP) is a new experimental protocol for

224 The first FDA-approved multiplex PCR panel for a large number of respiratory pa

225 A multiplex PCR panel targeting these five genes was used

226 We performed a clinical evaluation of a multiplex PCR panel, the enteric parasite panel (EPP), f

227 We evaluated a multiplexed PCR panel for the detection of 16 bacterial,

228 l phosphotriester modifications in improving multiplex PCR performance.

229 nt a novel and exciting avenue for improving multiplex PCR performance.

230 . falciparum and P. vivax in conventional or multiplex PCR platforms.

231 hm, and demonstrate its utility in compiling Multiplex PCR primer panels for detection and differenti

232 Twenty multiplex PCR/primer extension reactions were set up and

233 oftware package that automates the design of multiplex PCR primers for next-generation sequencing.

234 Eighteen sets of virus-specific multiplex PCR primers were developed based on the conser

235 ion PCR method involving the purification of multiplex PCR products followed by uniplex analysis on a

236 Multiplex PCR products were electrochemically detected i

237 nique, termed deligotyping, which hybridizes multiplex-PCR products to membrane-bound, highly specifi

238 We report the development of a multiplex PCR protocol for the diagnosis of staphylococc

239 A two-round multiplex PCR protocol was used to amplify these sequenc

240 were determined using several well-validated multiplex PCR protocols culled from the literature and s

241 equencing reaction, is used to interpret the multiplex PCR results.

242 Multiplex PCR revealed one tumor carried a homozygous de

243 es were serotyped by latex agglutination and multiplex PCR-reverse line blotting (mPCR/RLB).

244 al evaluating outcomes associated with rapid multiplex PCR (rmPCR) detection of bacteria, fungi, and

245 The assay consisted of a multiplexed PCR set of 5 tubes able to detect the most c

246 Multiplex PCR shows that the region including the G-quad

247 Furthermore, analysis by multiplex PCR shows that the types of mutants induced ar

248 ssessment of the SmaI restriction site-based multiplex PCR (SmaI-multiplex PCR) typing (SMT) with res

249 data from whole-genome projects, an updated multiplex PCR strategy was developed to assign Escherich

250 ecifically assigned 1 of 17 serotypes by the multiplex PCR system, with the results in complete conco

251 rogroup 6 isolates can be identified using a multiplex PCR system; however, due to the high sequence

252 d using an innovative strategy consisting of multiplexing PCR, targeted sequencing and computational

253 dentify CA-MRSA strains are based on complex multiplex PCRs targeting the staphylococcal cassette chr

254 e aggregative adherence fimbriae (AAFs) by a multiplex PCR, targeting the four known major subunit va

255 In conclusion, a multiplex PCR technique was developed for identifying fa

256 Using real time and multiplex PCR techniques, we identified three germline h

257 y have allowed for development of single and multiplexed PCR techniques that provide rapid detection

258 ults with results from a previously reported multiplex-PCR test (for T. pallidum, Haemophilus ducreyi

259 The results of a multiplex PCR that could detect 25 bacterial and fungal

260 We previously published a description of a multiplex PCR that detects A. actinomycetemcomitans and

261 By using a multiplex PCR that specifically amplifies several genes,

262 of human-pathogenic species, we developed a multiplex PCR that uses species-specific primers to ampl

263 m infection with six organisms identified by multiplex PCR that was initially thought to be a monomic

264 Nested Patch PCR, a novel method for highly multiplexed PCR that is very specific, can sensitively d

265 By multiplex PCR, the dominantly expressed form of human B7

266 As determined by a multiplex PCR, the E. coli ONT:H25 isolates carried a co

267 Using multiplex PCR, the prevalence of these 12 and 3 addition

268 Through the use of multiplexed PCR, the two mutation types were amplified i

269 we first applied tissue microdissection and multiplex PCR to detect homozygous deletion and/or hemiz

270 A multiplex PCR to differentiate S. hyicus, S. agnetis, an

271 Three sets of primers were used in one multiplex PCR to identify three different species.

272 Genomic DNA was amplified by multiplex PCR to produce amplicons of the three p53 exon

273 ntly involve tyrosine kinases, we designed a multiplex PCR to search for mRNA fusions between BCR and

274 ssay, a novel diagnostic tool which utilizes multiplex PCR to test for 21 respiratory pathogens with

275 I restriction site-based multiplex PCR (SmaI-multiplex PCR) typing (SMT) with respect to pulsed-field

276 reated patients using VNTR combined with 16S multiplex PCR unambiguously and reliably distinguished d

277 mavirus (HPV) genotyping assays are based on multiplex PCR using consensus or degenerate primers.

278 An improved multiplex PCR, using redesigned primers targeting the se

279 This multiplex PCR was compared to culture on 26 cervical swa

280 A multiplex PCR was designed to detect the eae gene and si

281 Accuracy of identification of the multiplex PCR was determined by comparisons to standard

282 The SmaI-multiplex PCR was found to be more discriminatory than M

283 ntibiotic susceptibility was determined, and multiplex PCR was performed for OXA-23-like, -24-like, -

284 The multiplex PCR was tested on isolated DNA or fungal colon

285 Multiplex PCR was then used to detect enterotoxin genes

286 An assay of multiplex PCR was used for the typing.

287 Multiplex PCR was used to compare the E. coli strains fo

288 Multiplex PCR was validated on the 384-well SlipChip wit

289 Multiplexed PCR was used to develop assays for genotypin

290 The limits of the multiplex PCR were 2.8 x 10(-2) CFU/microl PCR mixture f

291 Here, we report the development of a robust multiplex PCR which has assisted in the detection of 32

292 nical isolates of Acinetobacter species in a multiplex PCR, which also detects bla(OXA-23-like) and c

293 regions of each of these targets in a single multiplex PCR while incorporating a 6-carboxyfluorescein

294 Here we use a multiplex PCR with a mixture of primers targeting the re

295 nerally requires either latex agglutination, multiplex PCR with analysis of band sizes, or analysis o

296 s was developed that can unite the reward of multiplex PCR with real-time PCR to recognize animal gen

297 This is the first method that couples multiplex PCR with sample-specific DNA barcodes and next

298 TB-DzT combines a multiplex PCR with single nucleotide polymorphism (SNP)

299 methylation-sensitive restriction enzyme and multiplexed PCR with gene-specific primers (MSRE-PCR).

300 cific to different target genes are used for multiplex-PCR, with one primer for each target being lab