ライフサイエンスコーパス: mutagenesis

1 al load was associated with APOBEC-signature mutagenesis.

2 he ion pore that we confirm by site-directed mutagenesis.

3 tentially accurate alternative to laboratory mutagenesis.

4 ate structure and rationalize the results of mutagenesis.

5 rived HCV clone (Jc1Gluc2A) by site-directed mutagenesis.

6 id cells is sufficient to promote intestinal mutagenesis.

7 Pms2/Mlh1 is apparently dispensable for A-T mutagenesis.

8 f genomic stability against platinum-induced mutagenesis.

9 ity memory clones without undergoing further mutagenesis.

10 odulating promoter activity through targeted mutagenesis.

11 Smc3p subunit of cohesin by random insertion mutagenesis.

12 le of stochastic processes in DNA repair and mutagenesis.

13 replication forks as the cause of increased mutagenesis.

14 N8 NA subtypes using gene-fragmented random mutagenesis.

15 ationary phase contributes to stress-induced mutagenesis.

16 ites that were examined using phosphomimetic mutagenesis.

17 tion links nutrient deprivation to increased mutagenesis.

18 icism during neurodevelopment by insertional mutagenesis.

19 ed by a cysteine codon through site-directed mutagenesis.

20 MCD toward succinyl-CoA through active-site mutagenesis.

21 chanism by which p53 loss can promote cancer mutagenesis.

22 rgeted genome modification and site-directed mutagenesis.

23 oth allergens was performed by site-directed mutagenesis.

24 se, possibly limiting MMR to allow increased mutagenesis.

25 binding sites was confirmed by site-directed mutagenesis.

26 Using transposon mutagenesis, a gene, designated hmpF, was identified as

27 netic deletion of myomixer using CRISPR/Cas9 mutagenesis abolishes myoblast fusion in vivo.

28 stance mechanisms using genome-wide chemical mutagenesis allied to next-generation sequencing.

29 a catalytic model supported by site-directed mutagenesis allows full comparison with dicot CADs and e

30 L1s suggests that it is unlikely insertional mutagenesis alone accounts for the Mov10l1(-/-) phenotyp

31 osome and its RNA processing activity on DNA mutagenesis/alteration events have not been investigated

32 Further mutagenesis analyses indicated that Rrp2's Walker A and

33 enzymes involved and through structural and mutagenesis analyses, provide explanations of their cata

34 Combining transcriptome, bioinformatic and mutagenesis analyses, we delineate sequence determinants

35 Single-cell transcriptomics and mutagenesis analysis delineated dynamic molecular states

36 ication proteins can bind to sterol in vitro Mutagenesis analysis of p33 within the CRAC and CARC seq

37 have carried out an extensive site-directed mutagenesis analysis of the CD16A receptor complex and n

38 Mutagenesis analysis reveals two metal binding sites, te

39 ntrol in a protein backbone through external mutagenesis and a unique nanoscale platform to study str

40 subunit of the WRC previously identified by mutagenesis and biochemical data.

41 d by pressure was validated by site-directed mutagenesis and by inhibition by small peptides that mim

42 Functional, mutagenesis and complementation experiments were used to

43 Mutagenesis and crystallographic studies, including the

44 Mutagenesis and de novo TCR design confirmed that the GL

45 Our mutagenesis and electrophysiological studies, prompted b

46 using homology modeling in combination with mutagenesis and electrophysiology, we identified the bin

47 Here we used mutagenesis and enzyme kinetic analyses to address these

48 hich can be altered readily by site-directed mutagenesis and expressed in vitro and in vivo, the prep

49 mutation into a mouse strain by CRISPR-Cas9 mutagenesis and found that hemizygous mutants displayed

50 Mutagenesis and functional analyses using agonists to ma

51 drogen-deuterium exchange mass spectrometry, mutagenesis and functional studies.

52 Site-directed mutagenesis and gene rescue studies show that Akt-mediat

53 lly hazardous, as it can lead to insertional mutagenesis and genomic instability.

54 Using a random mutagenesis and growth selection approach, we identified

55 Here, we use comprehensive mutagenesis and hydrogen deuterium exchange mass spectro

56 ffects on BCC carcinogenesis-stimulatory via mutagenesis and inhibitory via production of hedgehog-in

57 that could not be attributed to insertional mutagenesis and instead likely resulted from expansion o

58 in/vinculin interaction, manipulated here by mutagenesis and mechanical control, is a core regulator

59 teolysis, gel electrophoresis, site-directed mutagenesis and microsecond MD simulations.

60 Moreover our NMR, mutagenesis and molecular dynamics simulation studies de

61 In this work, we performed mutagenesis and molecular modeling to strategically plac

62 Thus, random mutagenesis and mutational analysis allows for the achie

63 We used site-directed mutagenesis and patch-clamp recordings to probe the ion

64 The structure is confirmed by site-directed mutagenesis and provides a molecular framework for under

65 usly improved 10(5)-fold over many rounds of mutagenesis and screening to afford a proficient biocata

66 bility to decouple relative contributions of mutagenesis and selection in carcinogenesis, thus elucid

67 Using N-methyl-N-nitro-N-nitroso-guanidine mutagenesis and selection, a mutant strain Apmu4 was der

68 These results indicate that mutagenesis and skin carcinogenesis in IGF-1-deficient g

69 The mutagenesis and structural analysis allow us to propose

70 Based on a combination of mutagenesis and structural modeling, we suggest that all

71 and pre-steady-state kinetics combined with mutagenesis and structural studies to determine the mech

72 By site-directed mutagenesis and structure-guided analyses, we dissected

73 work universally, the efficiency of targeted mutagenesis and the adverse generation of off-target mut

74 son-mediated genome engineering, insertional mutagenesis and the genome-wide mapping of transcription

75 lysis of SSV1 using both specific and random mutagenesis and thereby generate mutations in all open r

76 l function, as demonstrated by site-directed mutagenesis and use of STAT3 knockout and mitochondria-d

77 into the host genome results in insertional mutagenesis and variable position effects.

78 genome-edited clones with minimal off-target mutagenesis and with indel mutation frequencies of 40-50

79 mbination of molecular dynamics simulations, mutagenesis, and A1-GpIbalpha binding experiments, we id

80 oxidation of proteins (FPOP), alanine shave mutagenesis, and binding analytics.

81 Comparative bioinformatics, mutagenesis, and biochemical analyses revealed that the

82 Here we use overexpression studies, mutagenesis, and flow cytometry to show that ICAP1 conta

83 cluding chemical modification, site-directed mutagenesis, and fluorescent spectroscopy, we demonstrat

84 performed by pepscan analysis, site-directed mutagenesis, and hydrogen/deuterium exchange-mass spectr

85 structures, molecular docking, site-directed mutagenesis, and kinetic and thermodynamic analyses.

86 rsibly altered by CRISPR/Cas9-based targeted mutagenesis, and later read out in single cells through

87 s, peptide binding analysis, linker-scanning mutagenesis, and nuclear magnetic resonance (NMR) studie

88 Here, we employed molecular modeling, mutagenesis, and patch clamp electrophysiology to elucid

89 MR, fluorescence spectroscopy, site-directed mutagenesis, and thermodynamics to elucidate the mechani

90 hips, using electrophysiology, site-directed mutagenesis, and voltage-clamp fluorometry.

91 Here we used a scanning mutagenesis approach to identify residues in the extrace

92 We report a general unnatural amino acid mutagenesis approach to quantify the contribution of ind

93 mutations, here we used a more comprehensive mutagenesis approach with the goal of identifying trimer

94 Using a mutagenesis approach, we dissected the energetic basis f

95 Our study suggests that strand-specific DNA mutagenesis-associated mechanisms will play major roles

96 d by computational results and site-directed mutagenesis at Ala33.

97 biologically programmed strand-specific DNA mutagenesis at focal DNA/RNA hybrid structures, they mak

98 aneous melanoma resolved novel signatures of mutagenesis attributable to ultraviolet radiation.

99 By combining site-directed mutagenesis, biochemical assays, and spectroscopic analy

100 dent nature of this process, conflict-driven mutagenesis can be used by cells to spatially (gene-spec

101 ation of the tumor suppressor protein p53 by mutagenesis, chemical modification, protein-protein inte

102 ecular dynamics simulation and site-directed mutagenesis confirmed the important roles of the key int

103 Site-directed mutagenesis confirms the observed interaction interface.

104 We also envisage that mutagenesis could be used to fine-tune the balance betwe

105 cerevisiae genome using saturated transposon mutagenesis coupled to high-throughput sequencing.

106 tivity, the choice of approach (for example, mutagenesis, CRISPR-based gene editing, RNA interference

107 the barrel is a deep pocket, which, based on mutagenesis data and amino acid conservation, comprises

108 ture-activity relationship and site-directed mutagenesis data facilitates the prediction of the struc

109 The high rate of base substitution mutagenesis demonstrated by our experiments is likely to

110 Combinatorial mutagenesis demonstrated that hydrophobic substitutions

111 catalytic activity and labeled four lysines; mutagenesis demonstrated that two of these, Lys-565 and

112 ting motor dimerization with structure-based mutagenesis drives dynein-1 into an open form with highe

113 For validation we used mutagenesis, electron microscopy and biochemical assays

114 In combination with integrated mutagenesis, ensemble-solution kinetics, and molecular d

115 predictions with outcomes of high-throughput mutagenesis experiments and measurements of human diseas

116 g protein expression, X-ray crystallography, mutagenesis experiments and molecular simulations.

117 n H-bonded network, which is corroborated by mutagenesis experiments as well as detailed atomistic si

118 within the mature peptide and site-directed mutagenesis experiments demonstrated that the first two

119 Mutagenesis experiments further validate simulation pred

120 Mutagenesis experiments indicated that PEP87 binds to it

121 ng effects and predictions for site-directed mutagenesis experiments.

122 nsmission that are fully consistent with the mutagenesis experiments.

123 as a model system and subjected it to random mutagenesis, followed by screening for variants whose in

124 loss-of-function as well as gain-of-function mutagenesis for functional gene annotation in vertebrate

125 We apply CREATE to site saturation mutagenesis for protein engineering, reconstruction of a

126 Fluorescence-reported allelic exchange mutagenesis (FRAEM) now enables the complete deletion of

127 Here, we combined high-throughput mutagenesis, functional screening, and deep sequencing t

128 e advanced to encompass transgenic, targeted mutagenesis, gene silencing (RNA interference), and geno

129 equencing technology coupled with saturation mutagenesis has provided new and global insights into ge

130 CRISPR saturation mutagenesis has the potential to dissect the functional

131 ude variants introduced into these sites via mutagenesis have limited effects on spectral tuning, yet

132 been mapped using photoaffinity ligands and mutagenesis; however, their precise target interaction p

133 f antibodies was studied by homolog-scanning mutagenesis (HSM) with single human domain human/porcine

134 anding genomic disorders, structural variant mutagenesis, human evolution, and cancer biology.

135 Systematic site-directed mutagenesis identified the core amino acid residues requ

136 Sequential mutagenesis identifies two evolutionarily conserved lysi

137 Molecular modeling and site-directed mutagenesis implicate several residues around the active

138 Previously, we performed random mutagenesis in a DeltaveA strain and identified revertan

139 by acute leukemia as a result of insertional mutagenesis in a high proportion of patients.

140 Constitutive PB mutagenesis in Arf(-/-) mice provided a collection of sp

141 egulated by nuclear architecture and mirrors mutagenesis in cancer.

142 A polymerase zeta activity was essential for mutagenesis in cisplatin-treated rad1Delta cells.

143 perturbation of this enhancer by CRISPR/Cas9 mutagenesis in hematopoietic stem and progenitor cells d

144 A quantum leap in our knowledge of mutagenesis in human cancers has resulted, stimulating a

145 s (AMLs) generated by retroviral insertional mutagenesis in Kras(G12D) "knockin" mice with the MEK in

146 These encounters increase mutagenesis in lagging-strand genes, where replication-t

147 Lack of an efficient system for targeted mutagenesis in obligates remains a major impediment in u

148 ovides a simple method to increase on-target mutagenesis in plants using CRISPR/Cas9.

149 as a major contributor to NM-Fapy-dG-induced mutagenesis in primate cells.

150 CRISPR/Cas9 induced high rates (88-100%) of mutagenesis in the target protein-encoding sites of MSTN

151 ses defining ionizing radiation (IR)-induced mutagenesis in vivo are poorly defined.

152 ower mutational load, lower proportion of UV mutagenesis, increased genomic stability, and harbor few

153 of the coiled-coil domains by site-directed mutagenesis increases the effective diffusivity of probe

154 o/ENSA, and phosphorylation site mapping and mutagenesis indicate that phosphorylation of Cdc55 contr

155 Site-directed mutagenesis indicates that tyrosine 645 in this loop has

156 phosphate of NADP(+) were probed by targeted mutagenesis, indicating that Thr-28, Ser-50, Arg-51, and

157 es and regulatory regions; thus, insertional mutagenesis is a substantial risk.

158 olyploid species, altering a trait by random mutagenesis is highly inefficient due to gene redundancy

159 by the genomic landscape and contributes to mutagenesis is unknown.

160 R intermediates, and elevated HRR-associated mutagenesis, is detectable in a yeast model of the human

161 Using site-directed mutagenesis, kinetic assays, and quantitative mass spect

162 ral analysis, combined with structure-guided mutagenesis, leads us to propose a catalytic mechanism t

163 relationship, we generated a site-saturation mutagenesis library covering each position along thiocil

164 enient system to investigate strand-specific mutagenesis mechanisms.

165 Compared with alternative site-directed mutagenesis methods, our protocol requires less sequenci

166 , we combine electrochemistry, site-directed mutagenesis, molecular dynamics and quantum chemical cal

167 mass spectrometry (MS), electron microscopy, mutagenesis, molecular dynamics simulations, and computa

168 Using mutagenesis, NMR, isothermal calorimetry and surface pla

169 Adenine-specific mutagenesis occurs during reverse transcription and does

170 everse-genetics pipeline enabled insertional mutagenesis of 18 of these 20 transcription factors.

171 Furthermore, site-directed mutagenesis of a predicted ETS-binding site within the C

172 Molecular modeling and site-directed mutagenesis of ARH3 revealed that numerous residues are

173 In this work, we performed alanine scanning mutagenesis of aromatic residues located in transmembran

174 eal the influence of chromatin on repair and mutagenesis of base lesions on a genome-wide scale and s

175 idue and illustrates the value of systematic mutagenesis of C. elegans for focused investigation of h

176 Mutagenesis of conserved residues with potential catalyt

177 Allelic replacement mutagenesis of cpt in T. denticola resulted in abrogatio

178 Mutagenesis of Cys-298 confirmed its role in dimerizatio

179 Mutagenesis of each of the putative K-turns singly or in

180 Alanine mutagenesis of each of the six phosphorylation sites was

181 By combining the mutagenesis of FKF1 with the optimization of a split FKF

182 tion, we performed a systematic alanine-scan mutagenesis of FoxP3, assessing mutational impacts on DN

183 copy, heme quantification, and site-directed mutagenesis of histidine residues, we demonstrated that

184 Segment reassortment and base mutagenesis of influenza A viruses are the primary route

185 ctivity in SNc dopaminergic neurons, whereas mutagenesis of lysine 68 to arginine (K68R), mimicking d

186 Site directed mutagenesis of lysine 68 to glutamine (K68Q), mimicking

187 of the AT1R was used to guide site-directed mutagenesis of outward-facing hydrophobic residues withi

188 Here, we performed in vitro saturating mutagenesis of pncA to generate a comprehensive library

189 such as interpreting/designing site-directed mutagenesis of proteins.

190 ity against acetyl-CoA, and structure-guided mutagenesis of putative active site residues identified

191 Site-directed mutagenesis of PxaTPS8 revealed several catalytic residu

192 Site-directed mutagenesis of RadH was used to identify catalytic resid

193 Site-directed mutagenesis of RCAR1 showed that its tyrosine residue is

194 Structure-guided mutagenesis of residues within the GDP-binding pocket id

195 we conducted an N-nitroso-N-methylurea (NMU) mutagenesis of S. viridis and screened for visible inflo

196 CRISPR/Cas9 system to conduct site-specific mutagenesis of SNCA in these cells, generating an isogen

197 Alanine mutagenesis of some of these residues disrupted the aNT-

198 receptor peptides followed by site-directed mutagenesis of the cleavage sites links receptor activat

199 Mutagenesis of the critical Y amino acids within the CRA

200 Mutagenesis of the Fn2-Fn2 interface increased the basal

201 Mutagenesis of the key interaction residues of Tie2 and

202 Further, site directed mutagenesis of the miR-134 binding site in Sabin-1 IRES

203 Site-directed mutagenesis of the phytaspase cleavage sites in prosyste

204 Structure-guided mutagenesis of the pore residues shows that they are cri

205 suppression is relieved through independent mutagenesis of the PTB domain and the CH1 tyrosine resid

206 Here we performed random mutagenesis of the RNA-guided Cas9 nuclease to look for

207 rcinoma A549 cells is rescued by CRISPR/Cas9 mutagenesis of the RNASEL gene or by expression of the R

208 Mutagenesis of the SNX5-PX:IncE binding surface disrupts

209 The combination of site specific mutagenesis of the three ZDHHC6 palmitoylation sites, ex

210 plays an important role during the strategic mutagenesis of the viral genome.

211 Site-directed (Arg-to-Ala) mutagenesis of this cleavage site abolished matriptase-m

212 or S531 conservation we performed systematic mutagenesis of this residue.

213 In this study, we made site-directed mutagenesis on the surface-exposed hydrophobic residues

214 segment corresponding to the fusion loop by mutagenesis or by antibodies blocks gamete fusion.

215 organisms, and what molecular mechanisms of mutagenesis or repair are required for this mutation-rat

216 l vectors prone to silencing and insertional mutagenesis or the use of nonhuman genes.

217 Reversible mutagenesis overcomes clonal variance by permitting func

218 ediated, high-throughput, saturating in situ mutagenesis permits fine-mapping of function across geno

219 Kinetic simulations and further mutagenesis provide evidence that ASICs show such steepl

220 The mechanisms of NM-induced mutagenesis remain unclear.

221 ications for heme transfer via site-directed mutagenesis, resonance Raman (RR), hydrogen-deuterium ex

222 acterial genes by sequence-driven saturation mutagenesis revealed a genome-wide map of the genetic de

223 Structure-guided mutagenesis revealed four catalytic residues that enable

224 down MS, NMR spectroscopy, and site-directed mutagenesis revealed specific and well-conserved posttra

225 Deletion mutagenesis revealed that a conserved motif in the C-ter

226 Site-directed mutagenesis revealed that GRK2 Ser-685 phosphorylation d

227 In vitro mutagenesis revealed that the differences in electrophor

228 Mutagenesis revealed that the dramatic differences in me

229 Site-directed mutagenesis revealed that the McpM precursor peptide inc

230 ients (n = 168), together with site-directed mutagenesis, revealed Nef position 9 as a subtype-specif

231 Structure-guided mutagenesis reveals that His175, Arg186, Thr276 and Tyr2

232 ted a sensitized N-ethyl-N-nitrosourea (ENU) mutagenesis screen for dominant thrombosuppressor genes

233 we developed a novel whole-body insertional mutagenesis screen in mice, which was designed for the d

234 We used a genome-wide transposon mutagenesis screen to identify where mutations were tole

235 Using a sensitized mutagenesis screen, we identified Smarca4 as a modifier

236 Yet, retroviral insertional mutagenesis screens identify RUNX genes as collaborating

237 in tractable model organisms using unbiased mutagenesis screens.

238 We used randomly barcoded transposon mutagenesis sequencing (RB-TnSeq) in Pseudomonas simiae,

239 Mass spectrometry and site-directed mutagenesis showed that chemically distinct HNO donors 1

240 Point mutagenesis showed that only the Class II motif contribu

241 Genetic tools and mutagenesis strategies based on transposable elements ar

242 We also show that similar mutagenesis strategies can be used to improve the yields

243 By using structure- and antigenicity-guided mutagenesis strategies focused on the V2 and V3 regions

244 At the same time, these systems enable novel mutagenesis strategies to introduce sequence diversity f

245 Bioinformatics analysis and mutagenesis strategies were consistent with the identifi

246 trategies, cofactors, protein scaffolds, and mutagenesis strategies.

247 A chemical mutagenesis strategy allowed a wide range of noncanonica

248 In this study, we used a transposon mutagenesis strategy based on a two-step Sleeping Beauty

249 evance of these amino acids using a targeted mutagenesis strategy.

250 tructure and similar sequences, we performed mutagenesis studies and determined the key role of the V

251 Furthermore, mutagenesis studies demonstrate that the glycosylation p

252 Finally, mutagenesis studies demonstrated residues in the back po

253 Deletion mutagenesis studies demonstrated that expression of a sh

254 Crystallographic and mutagenesis studies elucidated a key binding interaction

255 complex, but available crystal structures or mutagenesis studies have failed to identify such residue

256 Molecular modelling and mutagenesis studies indicate that agonist positive allos

257 A meta-analysis of mutagenesis studies indicated these predicted loops are

258 Based on our combined computational and mutagenesis studies of MhsT and LeuT, we propose that TM

259 Site-directed mutagenesis studies revealed four substitutions in OsMTP

260 Further mutagenesis studies revealed that HIV-1 Env, and the V3

261 Structure-activity relationship, docking and mutagenesis studies revealed the crucial interactions fo

262 Mutagenesis studies showed that tyrosine-774 is critical

263 Recent mutagenesis studies suggested a potential activation sit

264 Extensive site-directed mutagenesis studies supported the importance of this phe

265 trometry, combined with in vitro and in vivo mutagenesis studies, we identified the regions involved

266 TGATN4ATCAA-3' in these target sequences via mutagenesis studies.

267 The results of mutagenesis suggested that the transmembrane domains M1,

268 geTron insertion, we developed a CRISPR-Cas9 mutagenesis system for C. difficile.

269 ate the utility of GWA combined with reverse mutagenesis technologies to discover and validate genes

270 cDNAs were constructed by site-directed mutagenesis that encode PORB mutant proteins with define

271 We present evidence from atom-specific mutagenesis that neither the N1 nor N3 base positions of

272 Site-directed mutagenesis that swapped residue 153 between UGT89A2 fro

273 Furthermore, we show by site-directed mutagenesis that tyrosine (Y382-384) within the P2X7R C-

274 describe a protocol for 'post-translational mutagenesis' that enables the programmed installation of

275 Here we show, using synonymous mutagenesis, that CG suppression is essential for HIV-1

276 , through the use of noncanonical amino acid mutagenesis, that replacement of the proline residue at

277 Here we used genome-saturated mutagenesis to create a biobank of over 100,000 individu

278 e used truncation variants and site-directed mutagenesis to investigate domains and residues enabling

279 olytic footprinting, disulfide trapping, and mutagenesis to map the structures of ACKR3:CXCL12 and AC

280 Site-specific mutagenesis to prevent proteolytic processing generated

281 Importantly, we used mutagenesis to prove that the inhibition of TRAIL-induce

282 Here we used CRISPR/Cas9 somatic mutagenesis to test a patterning role for WntA, a signal

283 ges with genetic differences, and use CRISPR mutagenesis to validate that an intronic SNP in prominin

284 he evolution of genes and their potential as mutagenesis tools.

285 Moreover, Polzeta-dependent mutagenesis triggered by replisome defects or UV irradia

286 subclonal neoantigen burden, APOBEC-mediated mutagenesis, upregulation of cytolytic activity, and PD-

287 TAT4 function was evaluated by site-directed mutagenesis using a lymphoblastoid B cell line (B-LCL) a

288 additional mechanism to minimize the risk of mutagenesis, UVB-irradiated cells also activate a checkp

289 the FTIR spectra of wild-type enzyme and two mutagenesis variants, we are able to assign the IR signa

290 Site-directed mutagenesis was performed by partial gene synthesis, and

291 Site-directed mutagenesis was used to investigate the relative importa

292 Using truncation mutants and site-directed mutagenesis, we define the inhibitory face of the KA1 do

293 Through developmentally regulated targeted mutagenesis, we demonstrate that miR-219 alleles are cri

294 energy transfer technique, and site-directed mutagenesis, we examined the domains involved in HuR mul

295 molecular dynamics simulations, and protein mutagenesis, we gained insight into the molecular bases

296 on of in silico prediction and site-directed mutagenesis, we have mapped an exosite to a non-catalyti

297 Using site-directed mutagenesis, we validated those surfaces in vitro and in

298 ocalization were identified by site-directed mutagenesis, which identified serine residues 421 and 42

299 This system, termed memory by engineered mutagenesis with optical in situ readout (MEMOIR), is ba

300 ration of multiple homoeologs by CRISPR-Cas9 mutagenesis without any background mutations will offer