1 approach was employed, followed by a focused
mutagenesis study.
2 in GLAST has been delineated in an elaborate
mutagenesis study.
3 n (CCM), here we perform an alanine scanning
mutagenesis study.
4 cluding key residues predicted in a previous
mutagenesis study.
5 findings and those of previous experimental
mutagenesis studies.
6 ystallography and by conducting solution and
mutagenesis studies.
7 combined molecular docking and site directed
mutagenesis studies.
8 demonstrating excellent agreement with past
mutagenesis studies.
9 cal for binding to GIP through site-directed
mutagenesis studies.
10 mination have come from transposon insertion
mutagenesis studies.
11 ur predicted structure agrees with available
mutagenesis studies.
12 f substrate specificity were complemented by
mutagenesis studies.
13 to the assembly domain defined previously by
mutagenesis studies.
14 face was further supported by our additional
mutagenesis studies.
15 nd D146 have been implicated in catalysis by
mutagenesis studies.
16 with previous reports of bif2 phenotypes in
mutagenesis studies.
17 nt with predictions based on biochemical and
mutagenesis studies.
18 esult in different findings in whole-protein
mutagenesis studies.
19 l linkage analysis, electron microscopy, and
mutagenesis studies.
20 f the proteins and a number of site-directed
mutagenesis studies.
21 ture is consistent with previously published
mutagenesis studies.
22 TGATN4ATCAA-3' in these target sequences via
mutagenesis studies.
23 s first conducted, followed by site-directed
mutagenesis studies.
24 ed on lactose medium, Lac(-) strains used in
mutagenesis studies accumulate ampD beta-lactam resistan
25 A site-directed
mutagenesis study allowed the identification of three di
26 Single- and double-point amino acid
mutagenesis studies along with whole-cell electrophysiol
27 Mutagenesis studies also indicate that a phosphorylation
28 Mutagenesis studies also revealed that NQO1Y127/Y129 req
29 , contacting residues identified by previous
mutagenesis studies;
analogous behavior is observed in G
30 tructure and similar sequences, we performed
mutagenesis studies and determined the key role of the V
31 This was supported by
mutagenesis studies and experiments with alpha-conotoxin
32 Mutagenesis studies and fluorescence spectroscopy valida
33 Previous
mutagenesis studies and photoaffinity labeling using lig
34 This mechanism, further supported by
mutagenesis study and the structure of monofunctional gl
35 Substrates docking is validated by
mutagenesis studies,
and a structure-based catalytic mec
36 romoter sequence analyses, deletion studies,
mutagenesis studies,
and electrophoretic mobility shift
37 alorimetry (ITC), stopped-flow measurements,
mutagenesis studies,
and molecular dynamics (MD) simulat
38 in complex with a DNA substrate, followed by
mutagenesis studies,
and propose a common mechanism by w
39 sing secondary kinetic isotope effect (KIE),
mutagenesis study,
and primary KIEs.
40 ionship (SAR), crystallography, docking, and
mutagenesis studies are used to examine the binding mode
41 Mutagenesis studies as well as in vivo analyses of Notch
42 been developed on the basis of evidence from
mutagenesis studies,
as well as studies on the evolution
43 Mutagenesis studies,
based on both a computational inves
44 nique mechanism is provided by site-directed
mutagenesis studies,
biophysical characterization of the
45 With regard to the CR2-binding site on C3d,
mutagenesis studies by Isenman and coworkers have implic
46 Mutagenesis studies confirm that hydrophobic residues in
47 Mutagenesis studies confirm that the interface observed
48 Mutagenesis studies confirm that the ion pairs at the in
49 Mutagenesis studies confirm the contribution of the hydr
50 Biochemical and
mutagenesis studies confirm the stoichiometry and other
51 Mutagenesis studies confirmed a critical role for apoA1
52 Mutagenesis studies confirmed that Glu-16 is critical fo
53 Mutagenesis studies confirmed that polymorphisms at amin
54 Mutagenesis studies confirmed that Spider Roll binds the
55 faces formed by the two PD-1 ligands (PD-Ls)
Mutagenesis studies confirmed the details of the propose
56 The HS-binding site, identified by
mutagenesis study,
consists of eight basic residues that
57 NAAIRS
mutagenesis studies define amino acid sequences 181-199
58 mapping to the surface of the structure and
mutagenesis studies demarcated a hotspot likely to inter
59 Mutagenesis studies demonstrate that entry into this pro
60 Mutagenesis studies demonstrate that the [2Fe-2S] cluste
61 Furthermore,
mutagenesis studies demonstrate that the glycosylation p
62 cks sodium channels from the open state, and
mutagenesis studies demonstrate that this particle uses
63 Finally,
mutagenesis studies demonstrated residues in the back po
64 Furthermore, proteolysis and site-directed
mutagenesis studies demonstrated that 1G08 Fab binds a r
65 Mutagenesis studies demonstrated that a functional AF-2
66 Deletion
mutagenesis studies demonstrated that expression of a sh
67 Mutagenesis studies demonstrated that KIAA1549-BRAF fusi
68 Structure-based site-directed
mutagenesis studies demonstrated that R(91), at the tip
69 Mutagenesis studies demonstrated that Ser(47) within his
70 Site-directed
mutagenesis studies demonstrated that sites 1 and 4 have
71 Mutagenesis studies demonstrated that the S17 insert was
72 Structure guided
mutagenesis studies demonstrated that three salt bridges
73 Mutagenesis studies designed to probe for base-pairing i
74 In this
mutagenesis study,
each of the five base-specific amino
75 Crystallographic and
mutagenesis studies elucidated a key binding interaction
76 Bioinformatic analysis and
mutagenesis studies elucidated the biosynthetic pathway
77 mpounds, assessment of water energetics, and
mutagenesis studies enables SAR exploration to map GPCR-
78 Structure-guided
mutagenesis studies establish a crucial role for this su
79 Sequence analysis and
mutagenesis studies establish that this effect stems fro
80 trained based on a dataset of comprehensive
mutagenesis studies for 151 PPI complexes, with experime
81 elective resonance enhancement (for bCcO) or
mutagenesis studies (
for RsCcO).
82 Mutagenesis studies further confirmed that the interacti
83 Time-lapse imaging and
mutagenesis studies further establish a positive correla
84 Mutagenesis studies further established that Cys-199 is
85 Mutagenesis studies further map the functional interface
86 A site-directed
mutagenesis study,
guided by homology modeling to LuxR a
87 Published modeling and site-directed
mutagenesis studies had previously shown that the residu
88 Previous
mutagenesis studies had shown that MYOD1 with a p.Leu122
89 ture of a P2X4 receptor, in combination with
mutagenesis studies,
has provided a model of the intersu
90 Although previous
mutagenesis studies have demonstrated the importance of
91 Sequence analysis and
mutagenesis studies have elucidated a nucleotide sequenc
92 based protein footprinting and site-directed
mutagenesis studies have enabled us to map several inter
93 complex, but available crystal structures or
mutagenesis studies have failed to identify such residue
94 Recent
mutagenesis studies have identified a mutant G4C/S10C/T1
95 Protein engineering and
mutagenesis studies have suggested that the dissociation
96 Mutagenesis studies identified a single residue (positio
97 Mutagenesis studies identified a YYXXF sorting signal in
98 n, competitive inhibition, and site-directed
mutagenesis studies identified exosite 2 as the site of
99 Subsequent site-directed
mutagenesis studies identified five polar/charged residu
100 Mutagenesis studies identified interaction of the PIAS S
101 Site-directed
mutagenesis studies identified the amino terminus of the
102 Site-directed
mutagenesis studies identified three estrogen response e
103 In addition, these
mutagenesis studies identified three mutants, R786A, R82
104 A previous random
mutagenesis study identified a W165Y/E166Y/P167G triple
105 A recent
mutagenesis study identified transmembrane region (TM)4
106 Mutagenesis studies identify critical residues in the TM
107 Mutagenesis studies identify LspA as an aspartyl peptida
108 Substrate trapping and
mutagenesis studies identify PKM2 Tyr-105 and Tyr-148 as
109 Biochemical and
mutagenesis studies illustrate how CAR-mediated clusteri
110 Mutagenesis studies implicate Lys265 as the oxygen activ
111 Mutagenesis studies implicated the transmembrane regions
112 Prior site-directed
mutagenesis studies in bacterial ketosteroid isomerase (
113 co homology modelling, molecular docking and
mutagenesis studies in combination with substrate bindin
114 Mutagenesis studies in conjunction with structure-activi
115 This was confirmed by a site-directed
mutagenesis study in MDCK cells.
116 rtant binding sites were further analyzed by
mutagenesis studies,
in which corresponding vMIP-II muta
117 Molecular modelling and
mutagenesis studies indicate that agonist positive allos
118 Site-directed
mutagenesis studies indicate that amino acid residues pr
119 Mutagenesis studies indicate that H2O2 decreases CREB pr
120 Mutagenesis studies indicate that the compounds are orth
121 Mutagenesis studies indicate that the small molecules ac
122 Molecular pharmacology and
mutagenesis studies indicate that VU0255035 is a competi
123 Mutagenesis studies indicated FAK-His58 is critical for
124 Mutagenesis studies indicated that binding of Glu and Ca
125 o bind DNA as a homodimer, and site-directed
mutagenesis studies indicated that EbfC and its ortholog
126 In this study, complementation and
mutagenesis studies indicated that representatives of al
127 Mutagenesis studies indicated that the corresponding bac
128 o prediction of water network energetics and
mutagenesis studies indicated that the displacement of a
129 Mutagenesis studies indicated that the reactivity and sp
130 Site-directed
mutagenesis studies indicated that the transcription fac
131 Mutagenesis studies indicated that VRC01 contacts within
132 A meta-analysis of
mutagenesis studies indicated these predicted loops are
133 ing these existing approaches in large-scale
mutagenesis studies is limited by the technical challeng
134 Mutagenesis studies localized VEGF-A binding in the NRP1
135 ometry, and sequence-specific antibodies and
mutagenesis studies now unambiguously establish phosphor
136 Site-directed
mutagenesis studies of a strictly conserved T201 residue
137 Multiple site-directed
mutagenesis studies of apoA1 suggest that the pro-inflam
138 pe are monomeric proteins, and site-directed
mutagenesis studies of AtsB reveal that individual Cys -
139 Structural and
mutagenesis studies of Eis indicate that its acetylation
140 Biophysical, genome mapping, and
mutagenesis studies of FoxA1 reveals two different modes
141 of the HCT/HQT, we performed structural and
mutagenesis studies of HCT.
142 Site-directed
mutagenesis studies of highly conserved active-site resi
143 Site-directed
mutagenesis studies of K65R and T69del assessed the phen
144 is function have been determined by in vitro
mutagenesis studies of laboratory-adapted HIV-1 molecula
145 Based on our combined computational and
mutagenesis studies of MhsT and LeuT, we propose that TM
146 Based on structural and
mutagenesis studies of prokaryotic nucleic acid enzymes,
147 While previous
mutagenesis studies of PYDs point to the involvement of
148 Mutagenesis studies of the enzyme TbtD identified import
149 Deletion and
mutagenesis studies of the hrg-1 promoter revealed a 23-
150 ulation of Na(+),K(+)-ATPase was explored in
mutagenesis studies of the potential PKA site at Ser-938
151 Moreover,
mutagenesis studies of the receptor revealed key positio
152 immunoprecipitation (ChIP) and site-directed
mutagenesis studies of the RON promoter, we identified N
153 Mutagenesis studies of the UL11 N terminus revealed that
154 Mutagenesis studies of these residues in SARS-CoV S, fol
155 Mutagenesis studies of these sequences showed that bulky
156 Extensive
mutagenesis studies of three distinct TRBV11-2(+) TCRs f
157 Directed
mutagenesis studies of Xyn10D-Fae1A mapped the catalytic
158 Further, a
mutagenesis study of CgAcr3-1 suggested that a conserved
159 Mutagenesis study of PIP5K1A reveals two adjacent interf
160 In an elaborate
mutagenesis study of the 5-HT(3)A receptor guided by a h
161 313, and A316, were identified in a scanning
mutagenesis study of the BK (Ca(2+)-activated, large-con
162 A systematic site-directed
mutagenesis study of the Cys residues in the stem region
163 Thus, whole-protein
mutagenesis studies offer an acceptable means of identif
164 Systematic
mutagenesis studies on 100 mutants of fascin indicate th
165 is consistent with the limited site-directed
mutagenesis studies on 2B6 and extensive studies on P450
166 s (SeSaM followed by epPCR), site saturation
mutagenesis studies on individual positions, and one sim
167 Furthermore, our
mutagenesis studies on NES-H12 suggest that altered shut
168 We conducted
mutagenesis studies on several conserved residues that a
169 here can be used for the rational design of
mutagenesis studies on SEVI amyloid formation and viral
170 oxin family members, combined with extensive
mutagenesis studies on SubB, show how the hydrophobic pa
171 We report structural, computational, and
mutagenesis studies on the catalytic and resistance mech
172 Mutagenesis studies on the core Aib residue involved in
173 Site-directed
mutagenesis studies on this enzyme and its substrate had
174 y basic residues, we performed site-directed
mutagenesis studies on this enzyme, revealing that two a
175 Mutagenesis studies on three functionally impaired EC Ne
176 Mutagenesis studies on Tyr473 in helix 12 demonstrated t
177 and, in conjunction with the biochemical and
mutagenesis studies presented here, delineate the underl
178 t it accurately correlates with experimental
mutagenesis studies probing the mutational change in mea
179 NiR with a donor protein, AxNiR-cytc551, and
mutagenesis studies provide direct evidence for the impo
180 Computational and
mutagenesis studies provide strong support for a dominan
181 Our
mutagenesis studies provide the first experimental suppo
182 Mutagenesis studies reveal a delicate balance between ch
183 Docking and
mutagenesis studies reveal residues important for substr
184 Our bioinformatic analysis and
mutagenesis studies reveal that heterotetrameric ChsE1-C
185 Pulse-chases combined with
mutagenesis studies reveal that Ser1 strongly influences
186 Mutagenesis studies reveal that the clathrin binding mot
187 ecular dynamics simulations, biochemical and
mutagenesis studies reveal that the palmitoylation inser
188 Mutagenesis studies reveal that two extracellular residu
189 Structural analysis and complementary
mutagenesis studies reveal the basis for recognition and
190 ated a 580-bp human miR143/145 enhancer, and
mutagenesis studies revealed a critical role for both a
191 Site-directed
mutagenesis studies revealed four substitutions in OsMTP
192 Mutagenesis studies revealed that a C-terminal fragment
193 Mutagenesis studies revealed that Asp(202) and Glu(361)
194 Crystallographic and
mutagenesis studies revealed that distinct amino acids o
195 Detailed
mutagenesis studies revealed that each Sp site made a po
196 Further
mutagenesis studies revealed that HIV-1 Env, and the V3
197 Site-directed
mutagenesis studies revealed that imperatorin most likel
198 Conditional
mutagenesis studies revealed that MeCP2 dysfunction in e
199 Truncation and alanine
mutagenesis studies revealed that PP2Ac binds to the P3
200 Tandem mass spectrometry and
mutagenesis studies revealed that SREBP-1c is acetylated
201 Mutagenesis studies revealed that SSR42 codes for an 891
202 rometry analysis combined with site-directed
mutagenesis studies revealed that the lysine couple Lys(
203 Subunit-selective
mutagenesis studies revealed that the mutation in the p5
204 Mutagenesis studies revealed that the pilin-dependent mo
205 Although initial
mutagenesis studies revealed that the signature DEAD-box
206 Alanine scanning
mutagenesis studies revealed that the Thr-35 residue in
207 Mutagenesis studies revealed that these effects required
208 Subsequent
mutagenesis studies revealed that two residues in the ac
209 Structure-activity relationship, docking and
mutagenesis studies revealed the crucial interactions fo
210 Mutagenesis studies revealed the crucial role of the C-t
211 Structural and
mutagenesis studies revealed the molecular underpinnings
212 saminyl 6-phosphomethylmannoside, along with
mutagenesis studies,
revealed the residues involved in d
213 Structural, kinetics, and
mutagenesis studies show that CB7 binds to the aldehyde
214 X-ray crystallography and
mutagenesis studies show that mannose is ligated to the
215 Electrophysiology and
mutagenesis studies show that prokaryotic TRICs have sim
216 Site-directed
mutagenesis studies show that single-point mutations wit
217 Mutagenesis studies showed that an extra subdomain of ab
218 Mutagenesis studies showed that both hydrophobic and ele
219 Mutagenesis studies showed that covalent modification of
220 Site-directed
mutagenesis studies showed that Cys-805, Cys-930, and Cy
221 Remarkably, additional site-directed
mutagenesis studies showed that none of the lysines or h
222 Site-directed
mutagenesis studies showed that the core promoter and PA
223 Mutagenesis studies showed that three positively charged
224 Mutagenesis studies showed that tyrosine-774 is critical
225 Mutagenesis studies showed that variants of CjMan26A, fr
226 Site-directed
mutagenesis studies showed that Y361 and H412 were criti
227 Our A3G
mutagenesis study showed that lysine residues 297, 301,
228 Mutagenesis study showed that the ability of MCPIP1 to r
229 Homology modeling and
mutagenesis study showed that the locations of the carbo
230 two inhibitors are provided on the basis of
mutagenesis studies,
structure-activity relationship ana
231 Our
mutagenesis study subsequently identified VP1 C11 and C1
232 eta3 subunits as templates for site-directed
mutagenesis studies,
substitution with mbeta3 subunit re
233 Site-directed
mutagenesis studies suggest that at least predicted alph
234 Mutagenesis studies suggest that calmodulin binding to t
235 Mutagenesis studies suggest that minor changes in the en
236 Mass spectrometry and
mutagenesis studies suggest that phosphorylation of both
237 C1), a structurally related ion channel, and
mutagenesis studies suggest that the large extracellular
238 Mutagenesis studies suggest that the observed transcript
239 Molecular modeling and site-directed
mutagenesis studies suggest that the stimulatory compoun
240 However,
mutagenesis studies suggest that the tetrametric form of
241 Mutagenesis studies suggest that this group is histidine
242 Molecular modeling and site-directed
mutagenesis studies suggest that two residues in helix 7
243 Furthermore,
mutagenesis studies suggest that Val-499 is the primary
244 Mutagenesis studies suggest the electrostatic repulsion
245 Mutagenesis studies suggest the involvement of invariant
246 ons were determined and, in combination with
mutagenesis studies,
suggest that both Srr1 and Srr2 int
247 These data, along with
mutagenesis studies,
suggest that debranching (not inhib
248 Recent
mutagenesis studies suggested a potential activation sit
249 Mutagenesis studies suggested that both RING and B-box m
250 Further
mutagenesis studies suggested that mutation at position
251 Further
mutagenesis studies suggested this linker also plays an
252 This structural and
mutagenesis study suggests that VioE acts as a catalytic
253 steps in virion assembly, this comprehensive
mutagenesis study suggests yet another role for NS2 in l
254 This analysis, along with
mutagenesis studies,
suggests that the inhibitor binds a
255 Mutagenesis studies support the critical roles of Tyr9,
256 Extensive site-directed
mutagenesis studies supported the importance of this phe
257 fic adenosine 2'-O-methylation, rationalizes
mutagenesis studies targeting the K61-D146-K180-E216 enz
258 We have demonstrated in previous
mutagenesis studies that accumulation of the covalent co
259 relationship was confirmed by comprehensive
mutagenesis studies that also reveal the importance of i
260 ular dynamics simulations, and corroborative
mutagenesis studies,
the appropriate binding pockets for
261 Here we report, by systematic
mutagenesis studies,
the identification of E535 as part
262 A in its open ring form, in conjunction with
mutagenesis studies,
the potential substrate binding and
263 Through
mutagenesis studies,
the putative disulfide bond pairs i
264 Together with
mutagenesis studies,
these data suggest a model for subs
265 Together with docking and
mutagenesis studies,
these structures provide a comprehe
266 eport structural, biochemical and cell-based
mutagenesis studies to further characterize A3A's deamin
267 tly, this structure provides new targets for
mutagenesis studies to further understand and define thi
268 Previous
mutagenesis studies to identify the binding site on PAI-
269 2 and support the development of large-scale
mutagenesis studies to identify viral variants with uniq
270 ly in silico molecular modeling with in vivo
mutagenesis studies to investigate TCR-pMHC interactions
271 ms, we consider herein what the outcome of a
mutagenesis study truly reveals about an allosteric mech
272 Both forward and reverse
mutagenesis studies validate the impact of one or a few
273 structures of the F-BAR domain of Pacsin and
mutagenesis studies,
vesiculation could be linked to the
274 n the basis of these models, a site-directed
mutagenesis study was subsequently conducted that focuse
275 Using structural and site-directed
mutagenesis studies,
we demonstrate that ECG binds to th
276 Through
mutagenesis studies,
we demonstrate that phosphorylation
277 By truncation and
mutagenesis studies,
we demonstrated that the second alp
278 Then, guided by
mutagenesis studies,
we docked Dkk2C to the YWTD beta-pr
279 Through
mutagenesis studies,
we find that the ability of Org 275
280 omoter-luciferase system in combination with
mutagenesis studies,
we found that the polymorphic allel
281 By comparison with tyrosine recombinases and
mutagenesis studies,
we have been able to define some of
282 assay in Xenopus egg extracts and extensive
mutagenesis studies,
we have identified a highly conserv
283 tructural and sequence alignments as well as
mutagenesis studies,
we have identified the key residues
284 Through deletion and site-directed
mutagenesis studies,
we have identified Thr-680 as the m
285 y, homology modeling, molecular docking, and
mutagenesis studies,
we have located the substrate-bindi
286 Through
mutagenesis studies,
we identified crucial residues requ
287 ough bioinformatics, molecular modeling, and
mutagenesis studies,
we identified the putative NSC75609
288 trometry, combined with in vitro and in vivo
mutagenesis studies,
we identified the regions involved
289 Using truncation and
mutagenesis studies,
we mapped the auto-dephosphorylatio
290 Employing
mutagenesis studies,
we show that DDD could operate inde
291 Based on a comprehensive site-directed
mutagenesis study,
we localized the polySia attachment s
292 ion by the LBS of plasminogen, site-directed
mutagenesis studies were carried out using a construct e
293 ified with the two models, and site-directed
mutagenesis studies were conducted to validate the model
294 oscopy, molecular modeling, and double-cycle
mutagenesis studies were integrated to obtain a structur
295 modeling, species ortholog comparisons, and
mutagenesis studies were then employed to define the mol
296 Receptor chimera and site-directed
mutagenesis studies were used to validate these binding
297 reviously authenticated in gene transfer and
mutagenesis studies,
were compared to the orthologous mo
298 In addition, these
mutagenesis studies will aid in HDAC1-inhibitor design t
299 environment in spectral tuning by combining
mutagenesis studies with spectroscopic (UV-vis) and theo
300 Detailed
mutagenesis studies within this region revealed that fou