ライフサイエンスコーパス: mycothiol

1 berculosis, primarily as a building block of mycothiol.

2 GlcNAc-Ins from the medium and convert it to mycothiol.

3 idase is highly specific for S-conjugates of mycothiol.

4 the cell and utilized in the resynthesis of mycothiol.

5 M. smegmatis at a level about twice that of mycothiol.

6 d by MtAhpE in the presence of MR, Mrx1, and mycothiol.

7 covalent adducts with N-acetyl-cysteine and mycothiol.

8 m reflected by the ratio of reduced/oxidized mycothiol.

9 am-positive bacteria lacking glutathione and mycothiol.

10 ion of radiolabeled inositol 1-phosphate and mycothiol.

11 Mycothiol, 1-D-myo-inosityl-2-(N-acetylcysteinyl)amido-2

12 Mycothiol [2-(N-acetylcysteinyl)amido-2-deoxy-alpha-D-gl

13 The highly aerobic actinomycetes produce mycothiol, a conjugate of N-acetylcysteine with a pseudo

14 first enzyme involved in the biosynthesis of mycothiol, a major low-molecular-weight thiol in M. tube

15 Low molecular weight thiols, including a mycothiol analogue and thioredoxin, exerted a small but

16 n that a simplified cyclohexyl thioglycoside mycothiol analogue is a good substrate for MCA, it appea

17 onent correlated with defective recycling of mycothiol and accumulation of cellular oxidative damage.

18 cobacteria as a component of the major thiol mycothiol and also in the cell wall, with phosphatidylin

19 edoxin-1 (MtMrx1) acting in combination with mycothiol and mycothiol disulfide reductase (MR), as a b

20 These results indicate that mycothiol and mycothiol S-conjugate amidase play an impo

21 nce to oxidative stress and reacts with free mycothiol and mycothiolated targets.

22 peroxide detoxification actions inferred for mycothiol, and more recently, for Mrx1 in cellular syste

23 served that it demycothiolates and reduces a mycothiol arsenate adduct with kinetic properties differ

24 ermined by NMR the solution conformations of mycothiol bimane (MSmB) and the pseudodisaccharide 1-D-G

25 thiols, such as cysteine and an analogue of mycothiol, bind weakly to the [4Fe-4S] cluster, and expo

26 n of a chemical library of inhibitors of the mycothiol biosynthesis enzyme GlcNAc-Ins deacetylase (Ms

27 for indirect induction of mshB-D to support mycothiol biosynthesis is also presented.

28 The biochemical pathway of mycothiol biosynthesis is now fully elucidated.

29 A key step in mycothiol biosynthesis is the ATP-dependent ligation of

30 ts the first identification of a gene of the mycothiol biosynthesis pathway.

31 oup acetylated by acetyl-CoA to complete the mycothiol biosynthesis pathway.

32 The mshA deletion mutants were defective in mycothiol biosynthesis, were only ethionamide-resistant

33 -Ins is a key intermediate in the pathway of mycothiol biosynthesis.

34 gests that it may be important in regulating mycothiol biosynthesis.

35 form l-Cys-GlcN-Ins, the penultimate step in mycothiol biosynthesis.

36 l-1-phosphate in the first committed step of mycothiol biosynthesis.

37 Mycothiol biosynthetic and detoxification enzymes are no

38 mycothiol-S-conjugate amidase (MCA) and the mycothiol biosynthetic enzyme D-GlcNAc-alpha-(1 --> 1)-D

39 in a sigR null mutant, although no candidate mycothiol biosynthetic genes were identified among the s

40 The mycothiol biosynthetic pathway has been postulated to in

41 glucopyranoside (GlcNAc-Ins), a hypothetical mycothiol biosynthetic precursor.

42 r four weeks showed a reduction in levels of mycothiol, but phosphatidylinositol mannoside, lipomanna

43 Rv1170, a homolog of Rv1082, possesses weak mycothiol conjugate amidase activity but shows substanti

44 l mycothiol-dependent detoxification enzyme, mycothiol conjugate amidase, was recently identified in

45 namycins (Gdms B-G, including new linear and mycothiol conjugates), were characterized as metabolites

46 cterium smegmatis shown to be GlcNAc-Ins and mycothiol deficient was sequenced to identify a putative

47 It was shown that GlcNAc-Ins is absent in mycothiol-deficient mutant strain 49 of M. smegmatis and

48 In vivo, a mycothiol-deficient strain grew normally in immunodefici

49 of (35)S methionine into the cysteine adduct mycothiol, demonstrated the conversion of exogenous meth

50 enzyme GlcNAc-Ins deacetylase (MshB) and the mycothiol-dependent detoxification enzyme mycothiol- S-c

51 hat represent the natural substrates for the mycothiol-dependent detoxification enzyme mycothiol-S-co

52 A novel mycothiol-dependent detoxification enzyme, mycothiol con

53 A key mycothiol-dependent detoxification pathway utilizes the

54 MtMrx1, a glutaredoxin-like, mycothiol-dependent oxidoreductase, directly reduces the

55 al analyses revealed that Rv2466c is a novel mycothiol-dependent reductase, which represents a mycore

56 t is a potential drug target involved in the mycothiol detoxification pathway.

57 ss management and is oxidized to the dimeric mycothiol disulfide (MSSM) in the process.

58 x1) acting in combination with mycothiol and mycothiol disulfide reductase (MR), as a biologically re

59 NADPH-dependent mycothiol disulfide reductase (Mtr) helps to maintain an

60 dation of the two-electron reduced enzyme by mycothiol disulfide yielded a maximum rate of 190 +/- 10

61 in mshA demonstrate the non-essentiality of mycothiol for growth in vitro and in vivo, and provide a

62 How expression of mycothiol genes is regulated in mycobacteria has been un

63 Mycothiol has antioxidant activity as well as the abilit

64 The final three steps in the biosynthesis of mycothiol have been fully elucidated but the initial ste

65 intermediate in the biosynthetic pathway of mycothiol, i.e., 1-D-myo-inosityl-2-(N-acetyl-L-cysteiny

66 members of the Actinomycetes family produce mycothiol, i.e., 1-d-myo-inosityl-2-(N-acetyl-l-cysteiny

67 s to that established for the related thiol (mycothiol) in the Actinobacteria.

68 Mycothiol is a novel thiol produced only by actinomycete

69 Mycothiol is a novel thiol produced only by actinomycete

70 Mycothiol is an abundant small molecular weight thiol fo

71 Mycothiol is comprised of N-acetylcysteine (AcCys) amide

72 ciated with the membrane fraction, and since mycothiol is hydrophilic, direct reduction by MtMrx1 mig

73 MtMrx1 in aqueous intracellular media, where mycothiol is present at millimolar concentrations.

74 Mycothiol is the major thiol present in most actinomycet

75 Mycothiol is the major thiol produced by mycobacteria an

76 It was shown in vitro that mycothiol, like reduced thioredoxin and dithiothreitol,

77 regulator sigma(R) controls key elements of mycothiol metabolism is a major advance.

78 ese studies establish for the first time how mycothiol metabolism is regulated to cope with stress fr

79 nstrated that Mca is highly specific for the mycothiol moiety of mycothiol S-conjugates and relativel

80 between a thiol-dependent peroxidase and the mycothiol/Mrx1 pathway in Mycobacteria.

81 r members of the actinomycete family produce mycothiol (MSH or acetylcysteine-glucosamine-inositol, A

82 Mycothiol (MSH) (acetyl-Cys-GlcN-Ins) is the major low-m

83 of Mycobacterium smegmatis does not produce mycothiol (MSH) and was found to markedly overproduce bo

84 hione and instead utilize the small molecule mycothiol (MSH) as their primary reducing agent and for

85 ive bacteria that utilize the small molecule mycothiol (MSH) as their primary reducing agent.

86 Mycothiol (MSH) is the major low molecular weight (LMW)

87 Mycothiol (MSH) is the major low-molecular-mass thiol in

88 Mycothiol (MSH) is the major thiol in Actinobacteria and

89 Mycothiol (MSH) is the principal low-molecular-weight th

90 WhiB3, and the major M. tuberculosis thiol, mycothiol (MSH), are required to resist acidic stress du

91 or the determination of femtomole amounts of mycothiol (MSH), the main low-molecular-weight thiol in

92 Mycothiol (MSH; 1-D-myo-inosityl-2-(N-acetyl-L-cysteinyl

93 omycetes do not produce glutathione but make mycothiol (MSH; AcCys-GlcN-Ins) that has functions simil

94 Mycothiol ([MSH] AcCys-GlcN-Ins, where Ac is acetyl) is

95 Mycothiol, MSH or 1D-myo-inosityl 2-(N-acetyl-L-cysteiny

96 methionine sulfoxide reduction linked to the mycothiol/mycoredoxin-1 pathway.

97 y; rather, it receives electrons through the mycothiol/mycothione reductase/NADPH pathway to activate

98 Activity with 100 microM mycothiol or with the monobromobimane derivative of 1-D-

99 ase, MshA, is required for production of the mycothiol precursor, 1-O-(2-acetamido-2-deoxy-alpha-D-gl

100 Disulfide formation involving mycothiol probably competes with the direct reduction by

101 Mycothiol production was not detected in Propionibacteri

102 Using a genetic biosensor of mycothiol redox potential (EMSH), we demonstrated that a

103 e PknG mutant exhibited a reductive shift in mycothiol redox potential and compromised stress respons

104 onment resulted in higher oxidative shift in mycothiol redox potential of PknG mutant compared with t

105 The mycothiol S-conjugate amidase (amidase) responsible for

106 Mycothiol S-conjugate amidase (Mca) cleaves the amide bo

107 These results indicate that mycothiol and mycothiol S-conjugate amidase play an important role in

108 The mycothiol S-conjugate of rifamycin S was produced under

109 Significant activity was also seen with the mycothiol S-conjugate of the antibiotic cerulenin as a s

110 highly specific for the mycothiol moiety of mycothiol S-conjugates and relatively nonspecific for th

111 gate amidase (Mca) cleaves the amide bond of mycothiol S-conjugates of a variety of alkylating agents

112 pathway utilizes the amidase (Mca) to cleave mycothiol S-conjugates to produce GlcN-Ins and a mercapt

113 n its ability to react with a broad range of mycothiol S-conjugates, including two different classes

114 he mycothiol-dependent detoxification enzyme mycothiol- S-conjugate amidase (MCA) from Mycobacterium

115 he mycothiol-dependent detoxification enzyme mycothiol-S-conjugate amidase (MCA) and the mycothiol bi

116 could be adapted to measure the activity of mycothiol-S-conjugate amidase, a metal-dependent amidase

117 Mycothiol synthase (MshD) catalyzes N acetylation of Cys

118 The structure of the ternary complex of mycothiol synthase from Mycobacterium tuberculosis with

119 The mycothiol synthase mutant, the mshD::Tn5 mutant, produce

120 tructure of the ternary complex reveals that mycothiol synthase undergoes a large conformational chan

121 ene disruption in the mshD gene that encodes mycothiol synthase, the final enzyme in MSH biosynthesis

122 om thioredoxin reductase (TrxR) and not from mycothiol, the low molecular weight thiol of actinomycet

123 g agent monobromobimane (mBBr), the cellular mycothiol was converted to its bimane derivative (MSmB).

124 Mycothiol was previously shown to be synthesized from 1-

125 evel of the major actinomycete thiol buffer, mycothiol, was fourfold lower in a sigR null mutant, alt

126 e glutathione but make an alternative thiol, mycothiol, which has functions similar to those of gluta

127 oxidized MtAhpE forms a mixed disulfide with mycothiol, which in turn is reduced by MtMrx1 using a mo

128 reaction, MsrA forms a mixed disulfide with mycothiol, which is transferred via a thiol disulfide re

129 Conjugates of mycothiol with the antibiotic cerulenin, N-ethylmaleimid