ライフサイエンスコーパス: myosin I

1 be generated by actin polymerization and/or myosin I.

2 ctivated ATPase activity of purified adrenal myosin I.

3 domain in mediating the in vivo activity of myosin I.

4 odulin for in vitro motility of brush border myosin I.

5 ty-purified polyclonal antibodies to adrenal myosin I.

6 nct cellular functions of these two types of myosin-I.

7 solated Golgi stacks lack dynein but contain myosin-I.

8 vators of Arp2/3 complex, including WASP and myosin-I.

9 omain of WASp/Scar proteins and other fungal myosin-Is.

10 ins (TH1, 2, 3) found in typical long-tailed myosin-Is.

11 require the mechanochemical activity of the myosins-I.

12 We have determined that 95F myosin is a component of the IC whose function is essent

13 Myosin is a molecular motor responsible for biological m

14 Myosin is a motor protein whose functional unit in the s

15 We propose that 95F myosin is a motor that participates in membrane reorgani

16 gh interactions with anchoring proteins, and myosin is a protein kinase G-anchoring protein.

17 Although this myosin is a slower motor than wild-type myosin and has c

18 Conventional nonmuscle myosin-II (myosin) is a key chemomechanical motor that drives contr

19 though, the non-muscle myosin II holoenzyme (myosin) is a molecular motor that powers contraction of

20 Myosin 1b (Myo1b), a class I myosin, is a widely expressed, single-headed, actin-asso

21 situ inhibition of individual unconventional myosins is a powerful approach to determine their specif

22 We show that myosin I, a motor protein that has been implicated in po

23 The localization of myosin is abolished by a mutation in Cdc12p, implicating

24 ino-acid active site insert in phasic muscle myosin is absent from the tonic isoform.

25 When contractile myosin is absent, the normal Snail expression in mesoder

26 calculate a projection map of a "classical" myosin-I, Acanthamoeba myosin-IB (MIB), at approximately

27 etic analysis of SH3 sequences suggests that myosin-I acquired SH3 domain after the divergence of the

28 The motor function of smooth muscle myosin is activated by phosphorylation of the regulatory

29 Smooth muscle myosin is activated by regulatory light chain (RLC) phos

30 These interactions are broken when myosin is activated.

31 hought to contract when nonmuscle myosin II (myosin) is activated throughout a mixed-polarity actin n

32 ht chains phosphorylated (1P), smooth muscle myosin is active but its ATPase rate is <2P.

33 Phosphorylated myosin is also concentrated in cortical microfilament bu

34 This myosin is also present in axoplasm, as determined by two

35 Mediating the actomyosin interaction in myosin is an actin binding site distributed among severa

36 that clearly demonstrates that the class III myosin is an actin-based motor protein having a protein

37 Myosin is an actin-based motor protein that generates fo

38 We speculate that regulation of myosin is an ancestral characteristic of kinases in this

39 One of the major features of this myosin is an N-terminal PDZ domain that is included in s

40 Finally, we compared AMIC to brush-border myosin I and AMIB, which were previously studied under s

41 Western blot analysis showed that myosin I and myosin II were present in total pancreatic

42 hat actin dynamics are required in cells for myosin I and V motor proteins to transport their organel

43 al cells, contain both the actin-based motor myosin-I and dynein, whereas isolated Golgi stacks lack

44 To investigate the interplay between myosin-I and the canonical long-distance transport motor

45 Myosin-Is are molecular motors that link cellular membra

46 Some myosin-Is are proposed to act as force sensors, dynamica

47 We propose that subclass-1 myosin-Is are tuned for rapid sliding, whereas subclass-

48 re of these filaments, and the motor protein myosin is arranged on the core surface.

49 sive tension sensitivity supports a role for myosin I as a molecular force sensor.

50 of P(i) per mol) and activates Acanthamoeba myosin I as MIHCK does.

51 r EPR shows that the disorder of crosslinked myosin is at least 100 times slower.

52 When myosin is attached to actin in a muscle cell, various st

53 Brush border myosin-I (BBM-I) is a single-headed myosin found in the

54 Brush border myosin-I (BBM-I) is a single-headed unconventional myosi

55 ail domain differs greatly from brush border myosin-I (BBM-I), another member of the myosin-I class.

56 Brush border myosin I (BBMI) is a single-headed molecular motor.

57 Brush Border Myosin-I (BBMI) is a single-headed, unconventional myosi

58 The tail of whole myosin is bent sharply and consistently close to residue

59 This isoform of myosin I beta appears to be in a complex with RNA polyme

60 nd electron microscopy revealed that nuclear myosin I beta colocalized with RNA polymerase II in an a

61 A nuclear isoform of myosin I beta that contains a unique 16-amino acid amino

62 eviously implicated myosin 1c (M1c; formerly myosin I beta) in the retention of lamellipodia.

63 monoclonal antibody raised against mammalian myosin I beta.

64 ion regulator; reported separately), nuclear myosin I, beta-actin (reported separately), calponin 3,

65 reasing the fraction of the total cycle time myosin is bound to actin, resulting in a similar number

66 ns do not play a role in the localization of myosin I, but are absolutely required for in vivo functi

67 quired for in vitro motility of brush border myosin I, but AtKCBP is the first kinesin-related heavy

68 ictate the ATP hydrolysis cycle of mammalian myosin I by examining the properties of MI(1IQ).

69 These antibodies recognize adrenal myosin I by Western blot analysis (116 kDa) and inhibit

70 A group of closely related myosins is characterized by the presence of at least one

71 nventional myosins to be discovered, and the myosin-I class has since been found to be one of the mor

72 rder myosin-I (BBM-I), another member of the myosin-I class.

73 That myosin is cleaved in the absence of flightin is consiste

74 t for the notion that the release of Pi from myosin is closely associated with the generation of musc

75 The produced chimeric myosin is composed of the globular motor domain of skele

76 GFP-myosin is concentrated in the cleavage furrow during cyt

77 These results suggest that the amoeboid myosin I consensus phosphorylation site and SH3 domains

78 Notably included are nuclear myosin I-containing complexes that might sense and regul

79 between relay and converter domain of muscle myosin is critical for optimal myosin performance.

80 In vivo, myosin is crowded and constrained by the fiber lattice a

81 on promoting factors Wsp1p (WASp) and Myo1p (myosin-I) define two independent pathways that recruit A

82 As expected, macropinocytosis (myosin I-dependent), contractile vacuole activity (myosi

83 Myosin is detached by the actin binding of TnI, but TnI

84 The rate of myosin I detachment from actin decreases >75-fold under

85 e localization of mono- and diphosphorylated myosin is different from each other.

86 ironment of the nucleotide binding pocket of myosin is directly affected by a change of length applie

87 However, myosin I double mutants have significantly reduced (50%)

88 suggesting that the interaction of CaD with myosin is downregulated, at least in part, by the phosph

89 interaction between CaP and subfragment 1 of myosin is due to a direct binding of CaP to RLC.

90 ocused on the hyperflexible surface loops of myosin, i.e. loop 1 (ATPase loop) and loop 2 (actin bind

91 iting cross-bridge steps between EMB and IFI myosin--i.e., a myosin isomerization associated with MgA

92 Intramolecular communication within myosin is essential for its function as motor, but the s

93 nd those that mildly overexpress an amoeboid myosin I exhibit increased cortical tension.

94 Mutant Dictyostelium cells lacking two myosin Is exhibit profound defects in growth, endocytosi

95 nterpret these results to suggest that actin-myosin I exists in two forms in equilibrium, one of whic

96 Because each myosin is expressed in Drosophila indirect flight muscle

97 using a novel in vitro motility assay where myosin is extracted from single muscle fibre segments.

98 o1c is one of eight members of the mammalian myosin I family of actin-associated molecular motors.

99 Dictyostelium myoB, a member of the myosin I family of motor proteins, is important for cont

100 We find that two members of the myosin-I family, rat liver myosin-I of relative molecula

101 he widely expressed subclass-1 member of the myosin-I family.

102 We suggest that the vertebrate myosin-I field adopt a common nomenclature system based

103 f EMD 57033 to heat-inactivated beta-cardiac myosin is followed by refolding and reactivation of ATPa

104 ive description of the catalytic strategy of myosin is formulated, based on combined quantum-classica

105 te that the rod largely does not uncoil when myosin is free in solution, and at least beyond the firs

106 sent, and might account for the exclusion of myosin I from stress fibers.

107 The ATPase activity of myosin-Is from lower eukaryotes is activated by phosphor

108 minimal requirements of the tail region for myosin I function in vivo using the filamentous fungus A

109 In the absence of calcium, myosin is further inhibited by the binding of troponin-I

110 n, transformation of F. graminearum with the myosin I gene of Magnaporthe grisea, the causal agent of

111 lmodulin-binding IQ domains of the mammalian myosin I gene, rat myr-1 (130-kDa myosin I or MI(130)).

112 opose that the release rate of MgADP from V3 myosin is half that of V1 myosin without any difference

113 domain of vertebrate intestinal brush-border myosin I has been observed to swing through 31 degrees o

114 n of MYO3, a yeast gene encoding a "classic" myosin I, has no detectable phenotype.

115 Dictyostelium cells lacking only one myosin I have normal levels of cortical tension.

116 ls either lacking or overexpressing amoeboid myosin Is have significant defects in cortical activitie

117 The slower kinetics of myosin-I have allowed us to observe the separate mechani

118 The sequence homology between Acanthamoeba myosin I heavy chain kinase (MIHCK) and other p21-activa

119 Acanthamoeba myosin I heavy chain kinase (MIHCK) phosphorylates the h

120 null mutation lacking the PAK family member myosin I heavy chain kinase (MIHCK) shows mild chemotaxi

121 of the expressed 35-kDa catalytic domain of myosin I heavy chain kinase (MIHCK).

122 nine in the heavy chain is phosphorylated by myosin I heavy chain kinase (MIHCK).

123 Myosin I heavy chain kinase from Acanthamoeba castellani

124 The membrane association of a myosin I heavy chain kinase that regulates the activity

125 V(max) for the phosphorylation of Ser-329 by myosin I heavy chain kinase.

126 ploring important functional features of the myosin I heavy chain.

127 he baculovirus-expressed catalytic domain of myosin I heavy-chain kinase separated by gel electrophor

128 The relay domain of myosin is hypothesized to function as a communication pa

129 Once bound, myosin is hypothesized to potentiate the binding of furt

130 The cortical acto-myosin is identified as the main source of mechanical di

131 The importance of myosins is illustrated by the identification of myosin g

132 re at the head-rod junction of smooth muscle myosin is important for the phosphorylation-mediated reg

133 between streptococcal M protein and cardiac myosin is important in the pathogenesis of rheumatic hea

134 a, we conclude that the essential role(s) of myosin I in A. nidulans is probably structural, requirin

135 ere generated to further analyze the role of myosin I in these processes.

136 pression of a functional truncated mammalian myosin I in vitro, these results indicate that: 1) Ca(2+

137 tural elements in the catalytic domain while myosin is in complex with actin.

138 The binding site of Mts1 on myosin is in the rod region, particularly to the light m

139 K) phosphorylates the heavy chains of amoeba myosins I, increasing their actin-activated ATPase activ

140 the perimembrane region, whereas sarcomeric myosin is independently assembled into thick filaments o

141 on, many crawling cells can translocate when myosin is inhibited or absent.

142 rated by myosin contractile forces, but when myosin is inhibited, leading-edge membrane tension incre

143 nd the specific actin populations with which myosin-I interacts, is crucial to understanding the mole

144 site and lever arm, the structural state of myosin is intermediate between the weak-binding state pr

145 ro, we provide functional evidence that p200/myosin is involved in the assembly of basolateral transp

146 In mammalian cells, myosin-I is excluded from specific microfilament populat

147 Myosin-I is the single-headed member of the myosin super

148 Myosin-I is the single-headed member of the myosin super

149 Myosin-I is the single-headed, membrane binding member o

150 120-kDa protein is a previously undescribed myosin I isoform that is an intranuclear actin-based mol

151 Myo1b is a widely expressed myosin-I isoform that concentrates on endosomal and ruff

152 Myo1b is a widely expressed myosin-I isoform that concentrates on endosomal and ruff

153 amined force sensing by the widely expressed myosin-I isoform, myo1b, which is alternatively spliced

154 the single UCS protein (She4p) acts on both myosin-I isoforms (Myo3p and Myo5p) and one of two myosi

155 It is one of the best characterized myosin-I isoforms and appears to have unique biochemical

156 ural and biophysical studies have shown that myosin-I isoforms, Myosin-Ib (Myo1b) and Myosin-Ic (Myo1

157 this binding site is conserved within other myosin-I isoforms, suggesting they contain this putative

158 1e is > 10-fold faster than other vertebrate myosin-I isoforms.

159 domain after the divergence of the genes for myosin-I isoforms.

160 The three single-headed monomeric myosin I isozymes of Acanthamoeba castellanii (AMIs)-AMI

161 mark of the well-studied vertebrate class Va myosin is its ability to take multiple steps on actin as

162 the regulatory light chain (RLC) of cardiac myosin is known to play a beneficial role in heart disea

163 Although cardiac myosin is known to produce myocarditis in susceptible an

164 The folded tail of the whole myosin is less flexible, indicating interactions between

165 Therefore, in addition to myosin V, this myosin is likely to be an axoplasmic organelle motor.

166 The catalytic strategy described here for myosin is likely to be very similar in most nucleotide h

167 tivated ATPase activity observed in class II myosins is likely the result of Mg(2+)-dependent alterat

168 In vitro, capping protein Arp2/3 myosin I linker (CARMIL) antagonizes CP by reducing its

169 hich is the ortholog of human capping Arp2/3 myosin I linker (CARMIL), that localizes to the nonprotr

170 L proteins, for capping protein, Arp2/3, and myosin I linker.

171 s, PAKs and CARMIL (capping protein, Arp2/3, myosin I linker; a membrane-associated cytoskeletal scaf

172 here that CARMIL2 (capping protein, Arp2/3, myosin-I linker 2) provides such a molecular link.

173 role of CARMIL (capping protein, Arp2/3, and Myosin-I linker) family proteins in migrating cells.

174 protein CARMIL (capping protein, Arp2/3 and myosin-I linker) is a candidate.

175 Membrane association is essential for proper myosin-I localization and function.

176 on of functional redundancy among vertebrate myosins-I, may account for the lack of a whole animal ph

177 udies on human myosin-IXb indicate that this myosin is mechanochemically active and exhibits actin-bi

178 region (NTR) plays a critical role in tuning myosin-I mechanochemistry.

179 We conclude that the molecular assembly of myosin is mediated by the eukaryotic cytosolic chaperoni

180 yo1PH domains likely play universal roles in myosin I membrane association, but different isoforms ha

181 To better understand the dynamics of myosin-I membrane association, we measured the rates of

182 Biochemical experiments have shown that myosin-I membrane binding is the result of electrostatic

183 To better understand the mechanism of myosin-I-membrane association, we measured effective dis

184 The 130-kDa myosin I (MI(130)), product of the myr-1 gene, is one me

185 strated in a recent study, the single-headed myosin I molecule is an exquisite mechanosensor, able to

186 Phosphorylation of the myosin I molecules at this site regulates their enzymati

187 easured the displacement generated by single myosin I molecules, and we determined the actin-attachme

188 e motility or cortical contraction, multiple myosin-I molecules must be specifically localized to a s

189 Cardiac myosin is more radially displaced from the fiber axis.

190 ed on these results, we hypothesize that the myosin I motor protein facilitates the membrane fusion/v

191 myosin-mediated inhibition, ensuring maximal myosin-I motor activity at these sites.

192 otility is not a universal characteristic of myosin-I motors, as membrane-bound myosin-Ia (myo1a) and

193 ic coupling between ADP and actin binding to myosin is much smaller (K(AD)/K(D) approximately 5 inste

194 ortant, and suggest that previously reported myosin I mutant phenotypes in Dictyostelium may be due,

195 The coronin mutant and the myoA/B double myosin I mutant were more permissive than wild-type stra

196 on is perturbed by specific actin or nuclear myosin I mutants.

197 actin-filled cortex of various Dictyostelium myosin I mutants.

198 Here, we show that the sole S. pombe myosin I, myo1p, is required for proper organization of

199 ivo phosphorylation level of a Dictyostelium myosin I, myoB, was tested.

200 ensemble kinetic techniques to show that the myosin-I N-terminal region (NTR) plays a critical role i

201 Our studies demonstrate that a class VI myosin is necessary for basal protein targeting and spin

202 We now report that emerin binds nuclear myosin I (NMI, a molecular motor) directly in vitro.

203 At present, the myosin-I nomenclature is very confusing; not only are se

204 Our data indicate that myosin is not a substrate of Trim32; however, Trim32 was

205 Myosin is not involved in the latest stage of organelle

206 cy in the ability of yeast actin to activate myosin is not known.

207 ytoskeleton and core PCP components in which myosin is not simply a downstream target of PCP signalin

208 like membrane invaginations, indicating that myosin is not simply excluded from the periphery by some

209 ic fluorescence enhancement of smooth muscle myosin is not solely due to W512.

210 e mechanism of calcium regulation of scallop myosin is not understood, although it is known that both

211 ar mechanism underlying this contribution of myosin is not well understood.

212 motor function of vertebrate unconventional myosins is not well understood.

213 ly localized when they were expressed in the myosin I null mutants.

214 ys, and discovered that JS399-19 targets the myosin I of F. graminearum (FgMyo1).

215 wo members of the myosin-I family, rat liver myosin-I of relative molecular mass 130,000 (M(r) 130K)

216 transition of the force-generating region of myosin is only loosely coupled to the ATPase reaction, w

217 mammalian myosin I gene, rat myr-1 (130-kDa myosin I or MI(130)).

218 No interaction was found with myosin I or myosin V.

219 Antibodies specific to myosin I or to myosin II were used for immunocytochemist

220 d calmodulins, is represented by the 130-kDa myosin I (or MI(130)) from rat liver.

221 the hypothesis that the catalytic domain of myosin is orientationally disordered on actin in a post-

222 Amoeboid myosin Is play a critical role in regulating pseudopod f

223 ociation with periluminal actin suggest that myosin I plays a direct role in the process of transport

224 We report that a myosin is polarized anteriorly in these cells and strong

225 Protein isoform analysis revealed that beta-myosin is predominantly expressed even at the earliest t

226 We show that expression of this myosin is predominantly in the adult germ line and early

227 mber of the myosin-V class of unconventional myosins is present in axoplasm and optic lobes.

228 M(r) 130K) and chick intestinal brush-border myosin-I, produce movement in two distinct steps.

229 The molecular motor myosin is proposed to bind to actin and swing its light-

230 Remarkably, we discovered that the myosin I protein in fission yeast, Myo1, which is requir

231 play a role in phospholipid binding in other myosin-I proteins.

232 Smooth muscle myosin is regulated by phosphorylation of one of the two

233 In muscle, the assembly of sarcomeric myosin is regulated to produce stable, uniform thick fil

234 To investigate mammalian myosin I regulation, we have coexpressed in baculovirus

235 To determine the mechanism of myosin-I regulation by heavy-chain phosphorylation (HCP)

236 rtional to the initial ring radius if either myosin is released from the ring during contraction and

237 Thus this cleft-crosslinked myosin is remarkably similar, in both actin affinity and

238 Conventional myosin is representative of biomolecular motors in which

239 mitosis now reports that this unconventional myosin is required for pole integrity and normal spindle

240 il chimera indicate that this unconventional myosin is required for transit out of plasma membrane re

241 ng sites on F-actin so that binding of rigor myosin is required to fully activate the thin filament (

242 activity of motor proteins such as nonmuscle myosins is required for appropriate constriction of thes

243 emonstrated that the motor activity of these myosins is required for the polymerization step, and tha

244 stinct rotation of the light-chain domain of myosin is responsible for force generation and is couple

245 ntraction and actin filaments shorten, or if myosin is retained in the ring, while the actin filament

246 The amoeboid myosin I's are required for cellular cortical functions

247 sting that the contractile activity of these myosin I's is not controlled by this stimulus.

248 Given that the amoeboid myosin I's possess both actin- and membrane-binding doma

249 These results demonstrate that myosin I's work cooperatively to contribute substantiall

250 m that either lacks or overexpresses various myosin I's, using micropipette aspiration techniques.

251 The intrinsic fluorescence of smooth muscle myosin is sensitive to both nucleotide binding and hydro

252 The putative actin-binding interface of myosin is separated by a large cleft that extends into t

253 fusion proteins confirm the location of the myosin I SH3 domain binding site, implicate NH(2)-termin

254 progression in cells expressing a DeltaBLCBS-myosin is similar to that in wild-type cells.

255 This myosin is specifically cross-linked at SH1-SH2 by a chem

256 involvement of Acanthamoeba myosin I, yeast myosin I, STE20, PAK, and small GTP-binding proteins in

257 ating state has MgADP tightly bound, whereas myosin is strongly bound to actin.

258 actomyosin.ADP states and increases the time myosin is strongly bound to actin.

259 The Slam-dependent direct recruitment of myosin is sufficient to drive cleavage in the dunk mutan

260 wherein the normal stepping behavior of the myosin is supplemented by the preferential detachment of

261 that these domains are as long as the whole myosin-I tail in reconstructions of electron micrographs

262 We also show that a GFP-myosin-I-tail chimera expressed in epithelial cells is t

263 binding protein that shares with hair-bundle myosin I the properties of being photolabeled by vanadat

264 Myosin is the chemomechanical energy transducer in stria

265 of mavacamten-mediated inhibition of cardiac myosin is the decrease of phosphate release from beta-ca

266 Myosin is the enzyme performing ATP hydrolysis under the

267 Myosin is the molecular motor in muscle that generates t

268 Myosin is the molecular motor in muscle-binding actin an

269 Myosin is the most comprehensively studied molecular mot

270 The class III myosin is the most divergent member of the myosin superf

271 Myosin is the primary regulator of muscle strength and c

272 Myo2, a class-II myosin, is the major source of tension in the contractil

273 A hallmark of class-V myosins is their processivity--the ability to take multi

274 Myosin is thought to generate force by a rotation betwee

275 Myosin is thought to generate movement of actin filament

276 or predicted myosin genes including two new myosins-I, three new class II (conventional) myosins, a

277 inases, which activate the motor activity of myosin-I through phosphorylation.

278 Regulation of asymmetrically localized myosin is thus critical to ensure that furrow and spindl

279 PakB colocalizes with myosin I to actin-rich regions of the cell, including ma

280 The immunolocalization of myosin I to zymogen granule membranes and its close asso

281 ffect of OM on the mechano-chemical cycle of myosin is to increase the fraction of myosin molecules i

282 Therefore, for myosin-I to support processive motility or cortical cont

283 er myosin subclasses, might limit binding of myosins I to actin when actin-binding proteins, like tro

284 actors besides loop 4 determine targeting of myosins I to specific subpopulations of actin filaments.

285 nsion of 2 piconewtons or less, resulting in myosin I transitioning from a low (<0.2) to a high (>0.9

286 nuclear territories requiring nuclear actin/myosin-I transport machinery, dynein light chain 1 (DLC1

287 We conclude that, whether myosin is trapped at the actin-myosin interface or in th

288 To gain insight into how native myosin is tuned, we expressed three embryonic myosin iso

289 main in vivo disrupts tissue curvature where myosin is uniform.

290 Myosin is uniquely designed for such a role in that a lo

291 By immunocytochemistry, myosin I was also localized on isolated zymogen granules

292 it was of interest to determine whether each myosin I was directly associated with the plasma membran

293 in total pancreatic homogenate but that only myosin I was present on isolated zymogen granules and th

294 By immunocytochemistry, myosin I was shown in the apical aspect of acinar cells

295 d chicken myosin II as well as Dictyostelium myosin I, we observed a conserved pathway traversing swi

296 The Acanthamoeba castellanii myosin-Is were the first unconventional myosins to be di

297 Fission yeast myo1(+) encodes a myosin-I with all three tail homology domains (TH1, 2, 3

298 gical relevance of the direct association of myosin-I with phospholipids or about phospholipid headgr

299 mine the dynamics of MYO1A (M1A; formerly BB myosin I) within the BB using GFP-tagged MIA (GFP-M1A),

300 r with the known involvement of Acanthamoeba myosin I, yeast myosin I, STE20, PAK, and small GTP-bind