ライフサイエンスコーパス: myosin IIB

1 kinase C, a negative regulator of non-muscle myosin IIB.

2 ched in tropomodulin 1 (Tmod1) and nonmuscle myosin IIB.

3 This zone is also enriched for nonmuscle myosin IIB.

4 nd ERK, myosin light chain kinase (MLCK) and Myosin IIB.

5 as distinct from that of a nonmuscle myosin, myosin IIb.

6 cle myosin II, referred to as myosin IIA and myosin IIB.

7 ompensatory mechanism between myosin IIA and myosin IIB.

8 d states, which is in contrast to non-muscle myosin IIB.

9 the nonmuscle isoforms of alpha-actinin and myosin IIB.

10 little effect on the assembly properties of myosin-IIB.

11 However, in this study, we demonstrate that myosin IIB, a cytoplasmic myosin II particularly enriche

12 Here, we show that myosin IIB, a molecular motor that binds and contracts a

13 actin assembly at the leading edge, whereas myosin IIB accumulated in the rear 15-30 min later.

14 Pharmacologic or genetic inhibition of myosin IIB alters protrusive motility of spines, destabi

15 ults in an isoform switch from myosin IIC to myosin IIB and increased phosphorylation of myosin heavy

16 ntative of skeletal muscle myosin, nonmuscle myosin IIB, and nonmuscle myosin IIA revealed three dist

17 ntained fibrils composed of actin, nonmuscle myosin IIB, and sarcomeric alpha-actinin.

18 The restraint consists of myosin IIB- and IIA-dependent processes: retrograde acti

19 Conversely, the microinjection of myosin IIB antibody blocks microfilament-directed chromo

20 ts in the localized selective aggregation of myosin IIB but not myosin IIA at the region of parasite

21 However, posterior accumulation of myosin IIB, but not anterior distribution of myosin IIA,

22 hese observations indicate that asymmetry of myosin IIB, but not IIA, is regulated by light-chain pho

23 -specific control of the mechanosensation of myosin IIB, but not myosin IIA or IIC.

24 yosin II drives actin flow, and depletion of myosin IIB, but not myosin IIA, showed similar nondirect

25 m-specific manner, affecting the assembly of myosin-IIB, but not myosin-IIA.

26 unction of one of the isoforms of myosin II, myosin IIB, by analyzing the movement and mechanical cha

27 One of these putative substrates, myosin IIB, can be phosphorylated in vivo by Arg.

28 siRNA)-mediated suppression of myosin IIA or myosin IIB causes an increase in mitochondrial length in

29 alized MT organizing center (MTOC)/Golgi and myosin IIB cell rear enrichment.

30 shwork instability and collapse, followed by myosin IIB concentration at the rear of the cone and nec

31 We now demonstrate that myosin IIB contributes to tension at the epithelial ZA.

32 o develop convergence forces parallel to the myosin IIB-dependent dynamics of the actin cytoskeleton.

33 Suppressing nonmuscle myosin IIB disrupts directional cell rearrangements and

34 in IIA forms de novo filaments away from the myosin IIB-enriched center and back to form regions that

35 Interestingly, when myosin IIb expression and MyH7B expression were simultan

36 Abrogation of myosin IIB expression in the E1 knockdown cells has no e

37 indicate that transition between myosin IIC/myosin IIB expression is a critical feature of EMT that

38 n IIC is expressed in luminal cells, whereas myosin IIB expression is up-regulated in myoepithelial c

39 d in H-K-ATPase membrane trafficking include myosin IIB, F-actin, ezrin, and Rab GTPases.

40 r affinity for myosin-IIA filaments than for myosin-IIB filaments.

41 onses to Wnt5a involve recruitment of actin, myosin IIB, Frizzled 3, and melanoma cell adhesion molec

42 t did not affect protrusion, suggesting that myosin IIB functions in pulling the rear of the cell for

43 Cells depleted of myosin IIB, however, were efficient in thrombin-induced

44 ced lamellar spreading, whereas depletion of myosin IIB impaired not only migration but also impaired

45 e polarized localization of MCAM, actin, and myosin IIB in a Wnt5a-induced manner.

46 ongate the body axis, to examine the role of myosin IIB in convergence and extension.

47 have found that MLC-dependent activation of myosin IIB in migrating cells is required to form an ext

48 correlates the recruitment of myosin IIA and myosin IIB into this spreading margin.

49 Although myosin IIB is also found at the zonula adherens (ZA) in

50 ion analysis reveals for the first time that myosin IIB is associated with vimentin, linking vimentin

51 Myosin IIB is enriched in the postsynaptic density (PSD)

52 ht on the mechanism, showing that non-muscle myosin IIb is intimately involved.

53 Our results suggest that myosin IIB is involved not in propelling but in directin

54 We find that myosin IIB is localized in the cortex of intercalating c

55 Therefore, myosin IIB is necessary for normal growth cone spreading

56 In the superficial layer, myosin IIB is needed for apical actin accumulation, whic

57 We show that myosin IIB is required for actin-cytoskeletal organizati

58 We also show that myosin IIB is required for resistance to deformation ("s

59 that a short serine-rich motif in nonmuscle myosin IIB is required to establish the cell's rear.

60 To test whether myosin IIB is responsible for the force generation, we q

61 We show that nonmuscle MIIB (myosin-IIB) is unpolarized in cells on soft matrix in 2D

62 mediated traction force in growth cones from myosin IIB knock-out (KO) mice and compared them with ne

63 y with blebbistatin or by using neurons from myosin IIB knockouts inhibits retraction.

64 wed, but not eliminated, in neurons from the myosin IIB KO mice.

65 with emerin, and emerin depletion prevented myosin IIB localization near nuclei.

66 Activated myosin IIB localizes prominently at the cell rear and pr

67 l family kinase substrates and suggests that myosin IIB may be regulated by tyrosine phosphorylation.

68 that the individual nonprocessive nonmuscle myosin IIB molecules form a highly processive unit when

69 issue-targeting techniques, we show that the myosin IIB motor protein complex is essential for both t

70 power stroke, specifically activating human myosin IIB (MYH10) and human myosin IIC (MYH14), but not

71 2, beta-catenin (CTNNB1), N-cadherin (CDH2), myosin IIB (MYOIIB), aPKCzeta, LGL, PAR3, pericentrin, P

72 Non-muscle myosin IIB (NMIIB) generates tension along actin filamen

73 Nonmuscle myosin IIB (NMIIB) is a cytoplasmic myosin, which plays

74 ry of a viable therapeutic target, nonmuscle myosin IIB (NMIIB), a molecular motor that supports memo

75 chain IIB (NMHCIIB), a subunit of nonmuscle myosin IIB (NMIIB), as an ER stress-dependent interactin

76 of myo1b and the motor domain from nonmuscle myosin-IIb (nmMIIb) concentrates on actin filaments in r

77 Similar to non-muscle myosin IIB, non-muscle myosin IIA shows high ADP affinit

78 Myosin IIB null cells displayed multiple unstable and di

79 te bending, whereas in the deep neural cells myosin IIB organizes a cortical actin cytoskeleton, whic

80 ic stem and progenitor cells, with polarized myosin-IIB promoting asymmetric self-renewal and constit

81 Myosin IIB redistributes from a broad distribution to th

82 al for the mechanics of cell migration, with myosin IIB seeming to have a preferential role in the me

83 These features imply that myosin IIB serves a set of physiologic needs different f

84 Transient kinetics of non-muscle myosin IIB showed that this motor has a very high actomy

85 atin or selective RNA-mediated repression of myosin IIB significantly inhibits (P < 0.05) C. parvum c

86 Myosin IIB specifically coimmunoprecipitated with emerin

87 tatin on the kinetic properties of nonmuscle myosin IIB subfragment 1 (NMIIB S1).

88 netic characterization of a human non-muscle myosin IIB subfragment-1 construct produced in the bacul

89 Thus, non-muscle myosin IIB subfragment-1 spends a significantly higher p

90 in the offspring and increased the ratio of myosin IIb to other isoforms by 17.6 +/- 4.9% (P < 0.05)

91 hese results show that emerin functions with myosin IIB to polarize actin flow and nuclear movement i

92 e-Rap1 pathway as responsible for recruiting myosin IIB to the ZA and supporting contractile tension.

93 In contrast, genetic ablation of non-muscle myosin IIB was associated with a 60% decrease in mitocho

94 Immunofluorescence staining indicated that myosin IIB was localized preferentially along stress fib

95 Posterior accumulation of myosin IIB was unaffected.

96 s of CARMIL2 also caused decreased levels of myosin-IIB, which may contribute to the polarity phenoty

97 in COS7 cells disrupted the localization of myosin IIB without obviously affecting actin filaments.