ライフサイエンスコーパス: myosin III

1 ated interactions between Arr2 and the NINAC myosin III.

2 indicating the mechanoenzymatic activity of myosin III.

3 er inactivation nor afterpotential C (NINAC) myosin III, a motor protein/kinase, also display a simil

4 This phosphorylation may modulate myosin III-actin interactions.

5 sites that become phosphorylated in Limulus myosin III and investigated its kinase, actin binding, a

6 We seek to understand the role of Limulus myosin III and its phosphorylation in photoreceptors.

7 Opsin, visual arrestin, myosin III, and calmodulin are all concentrated at the p

8 out the functions of opsin, visual arrestin, myosin III, and calmodulin in photoreceptors and set the

9 against Limulus opsin, visual arrestin, and myosin III, and we have used them to examine the distrib

10 that interactions between actin and Limulus myosin III are regulated by both second messenger mediat

11 Finally, we demonstrate that Limulus myosin III binds actin but lacks ATPase activity.

12 present in developing stereocilia, is also a myosin-III cargo and is essential for normal hearing.

13 g espin-1 (ESPN-1), an isoform of ESPN and a myosin-III cargo, dramatically alters the slope of the s

14 ect demonstration of kinase activity for the myosin III class.

15 Myosin III containing calmodulin as the light chain subu

16 We report here that myosin III does exhibit protein kinase activity.

17 We show that Limulus myosin III exhibits kinase activity and that a major sit

18 n the actin interface of full-length Limulus myosin III expressed in baculovirus are substrates for b

19 ently reported the cloning of MYO3A, a human myosin III expressed predominantly in the retina and ret

20 and show here that it is a new member of the myosin III family.

21 Two members of the myosin-III family, MYO3A and MYO3B, are thought to regul

22 tion might play a role for the regulation of myosin III function.

23 , actin co-sedimentation assays suggest that myosin III has a relatively high steady-state affinity f

24 Furthermore, we found that human myosin III has actin translocating activity (0.11 +/- 0.

25 Here, we showed that human myosin III has an ATPase activity that is significantly

26 he physiological and biochemical function of myosin III has not characterized.

27 ants lacking NINAC (calmodulin [CaM] binding myosin III) in the cell body, translocation remained rap

28 gulatory light chain (LC20), suggesting that myosin III is a multifunctional protein kinase.

29 The phosphoamino acid analysis revealed that myosin III is a serine/threonine kinase but not a tyrosi

30 We conclude that Limulus myosin III is an actin-binding and signaling protein and

31 propose that the phosphorylation of Limulus myosin III is involved in one or more of the structural

32 Limulus myosin III is particularly interesting because it is a p

33 We report that Limulus myosin III is similar to other unconventional myosins in

34 Our data also suggest the myosin III motor spends a large fraction of its cycle in

35 t, the rhabdomere, required the eye-enriched myosin III, NINAC.

36 tants of calmodulin (CaM) or the CaM-binding myosin III, NINAC.

37 homologous proteins which are classified as myosin III of the myosin superfamily, yet the physiologi

38 Opsin and visual arrestin, but not myosin III or calmodulin, are also concentrated in extra

39 hosphorylated a number of proteins including myosin III p132 and smooth muscle myosin regulatory ligh

40 hese findings demonstrate in vivo changes in myosin III phosphorylation in response to a natural stim

41 We show that the kinase domain of Limulus myosin III shares some pharmacological properties with p

42 The observation that MYOIIIPK phosphorylates myosin III suggests that the autophosphorylation might p

43 DP state may be important for the ability of myosin III to function as a cellular transporter and act

44 The kinase homologous domain (MYOIIIPK) of myosin III was expressed in the baculovirus expression s

45 In addition, visual arrestin and myosin III were found widely distributed in the cytosol

46 trated that INAD bound directly to the NINAC myosin III, yet the subcellular localization of NINAC wa